Rh4AG056900

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0006945
Physical Location & Seq
Reverse (-)
12079780 .. 12082422
2643 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG056900.1

Sequence Viewer

Length: 1251 bp
ATGACTCAAAGGAACACCGTCACATACAACTCCATGATCTCCGCCTACGGCAAGAACCGGTACCTGCACAGTAATATGGTGGAGGATGCTTATGGGCTTTTTGTTCACATGCCACAAAGAGACCTTTTCTCTTGGACTTTGATGATTACTTGCTACACACGTAACGGCGAGCTTGACAGAGCCAGACAGCTGTTCAGTTTGCTTCCGGATAAGCGGGACCCGGCTTGTTGGAATGCCATGATTGCAGGGTATGTGAAGAAGAGGAGGTTTGAGGATGCGAAGAGAATGTTTGATGAAATGCCGGTTGGTGAGTTGGATAATGCTTGGAAGTTTTTTGAGAAGATTCCGGAGCCTAATGTTGTTTCGTGGGTGACAATGTTGTGTGGGTTTGCACAAAATGGGAAGATTGCGCAGGCGGAGGATCTGTTTGAGAAGATGCCGAGTAGAAGTGTGGTTGCTTGGAATGCAATGCTTGCAGCCTATGTACAAGACCGCCGAATTGACAAGGCTGTTGAAATATTCAGGGATATGCCGGAGATGGATTCAGTGTCGTGGACTACAATGATCAATGGGTATGTTCGCGTTGGCAAGCTTGACGAAGCAAGGCAGTTGCTCAACCGGATGCCATACAGGAACATTGCAGCACAAACAGCAATGATATCTGGATATGCTCATAATGGAAGGATGGATGAAGCAAGTCAGATCTTCAATCAGATTGCCATGCGTGATGTTGTTTGTTGGAACACCATGATTGCAGGCTATGCTCAGTGTGGCAGAATGGTTGAAGCTCTATCTCATTTCAGAAACATGACCAACAAGGATGTAGTTTCTTGGAACACTCTGATTACTGGTTATGCTCAAGTAGGGGAGATGGACAAAGCACTTCAAATCTTTGAGGAGATGGGGGAGAAGAACATAGTTTCTTGGAATTCTCTAATTACAGGATATGTGCAGAACGGGTTATATCTGGATGCACTGAAGAGTATTGTGATTATGGATCAGGAAGGAAAGAGGCTTGATCAATCAACTTTTTCATGTGGTCTAAGTGCATGTGCCAATCTTGCAGCTTTTCAACTGGGAAGGCAACTTCACCAAATGGTTGTGAAGACCGGCTATCTAAGGTACTATAAGTCTATAAATGGACGCGGAGGATCCACTGGAAACAAGTTCAGGATGTCACTGGGTCTGCCAGTGGCAGCCACAGTGAACTGTGCTGACAATACAGGAGCTAAGAACCTGTACATCATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR

Protein Analysis

416

Amino Acids

47.3

Weight (kDa)

8.76

Isoelectric Point (pI)

35.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 75 - 100 4.1e-06 PPR repeat family
PPR PF01535 76 - 100 6.1e-07 PPR repeat
PPR_1 PF12854 117 - 146 5.7e-06 PPR repeat
PPR_2 PF13041 118 - 151 2.7e-06 PPR repeat family
PPR_2 PF13041 150 - 179 2.9e-06 PPR repeat family
PPR PF01535 152 - 180 2.4e-06 PPR repeat
PPR_1 PF12854 180 - 208 4.6e-07 PPR repeat
PPR_2 PF13041 181 - 213 1e-07 PPR repeat family
PPR PF01535 183 - 212 2.4e-08 PPR repeat
PPR_2 PF13041 242 - 274 8.2e-07 PPR repeat family
PPR PF01535 245 - 274 8.4e-07 PPR repeat
PPR_2 PF13041 273 - 306 1.1e-09 PPR repeat family
PPR_1 PF12854 274 - 301 6.3e-08 PPR repeat
PPR_3 PF13812 274 - 318 4.6e-06 Pentatricopeptide repeat domain
PPR PF01535 276 - 306 2.1e-09 PPR repeat
PPR_2 PF13041 304 - 353 5.9e-07 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 411
Acc36I ACCTGC 1 cut(s) 72
Acc65I GGTACC 1 cut(s) 60
AccB1I GGYRCC 1 cut(s) 60
AccII CGCG 2 cut(s) 582, 1146
AccIII TCCGGA 2 cut(s) 205, 346
AciI CCGC 5 cut(s) 42, 214, 416, 493, 1146
AclWI GGATC 4 cut(s) 429, 1005, 1146, 1159
AcsI RAATTY 1 cut(s) 928
AcuI CTGAAG 1 cut(s) 998
AfaI GTAC 4 cut(s) 62, 486, 1124, 1241
AflIII ACRYGT 1 cut(s) 158
AgeI ACCGGT 1 cut(s) 57
AgsI TTSAA 5 cut(s) 515, 709, 785, 887, 1073
AjuI GAANNNNNNNTTGG 2 cut(s) 288, 320
AluBI AGCT 6 cut(s) 172, 190, 592, 788, 1067, 1229
AluI AGCT 6 cut(s) 172, 190, 592, 788, 1067, 1229
Alw26I GTCTC 1 cut(s) 114
AlwI GGATC 4 cut(s) 429, 1005, 1146, 1159
Aor13HI TCCGGA 2 cut(s) 205, 346
ApeKI GCWGC 4 cut(s) 476, 641, 1064, 1196
ApoI RAATTY 1 cut(s) 928
AsiGI ACCGGT 1 cut(s) 57
Asp718I GGTACC 1 cut(s) 60
AspLEI GCGC 1 cut(s) 412
AspS9I GGNCC 1 cut(s) 217
AsuC2I CCSGG 1 cut(s) 221
AsuHPI GGTGA 3 cut(s) 320, 382, 1082
AvaII GGWCC 1 cut(s) 217
BamHI GGATCC 1 cut(s) 1151
BanI GGYRCC 1 cut(s) 60
BbsI GAAGAC 1 cut(s) 1112
BbvI GCAGC 4 cut(s) 488, 653, 1076, 1208
BccI CCATC 4 cut(s) 532, 679, 865, 895
BceAI ACGGC 2 cut(s) 64, 181
BclI TGATCA 2 cut(s) 564, 1018
BcnI CCSGG 1 cut(s) 221
BcoDI GTCTC 1 cut(s) 114
BfuAI ACCTGC 1 cut(s) 72
BglII AGATCT 1 cut(s) 702
BisI GCNGC 4 cut(s) 477, 642, 1065, 1197
BlsI GCNGC 4 cut(s) 478, 643, 1066, 1198
Bme1390I CCNGG 1 cut(s) 221
Bme18I GGWCC 1 cut(s) 217
BmgT120I GGNCC 1 cut(s) 217
BmiI GGNNCC 5 cut(s) 62, 218, 219, 351, 1153
BmrFI CCNGG 1 cut(s) 221
BmrI ACTGGG 2 cut(s) 1085, 1190
BmsI GCATC 5 cut(s) 76, 265, 426, 612, 961
BmuI ACTGGG 2 cut(s) 1085, 1190
BpiI GAAGAC 1 cut(s) 1112
BpuEI CTTGAG 1 cut(s) 843
BpuMI CCSGG 1 cut(s) 221
BsaAI YACGTR 1 cut(s) 161
BsaI GGTCTC 1 cut(s) 114
BsaWI WCCGGW 4 cut(s) 57, 205, 346, 618
BsaXI ACNNNNNCTCC 2 cut(s) 410, 440
Bse118I RCCGGY 3 cut(s) 57, 301, 1109
Bse1I ACTGG 5 cut(s) 853, 1080, 1162, 1185, 1190
Bse3DI GCAATG 3 cut(s) 474, 636, 660
BseAI TCCGGA 2 cut(s) 205, 346
BseGI GGATG 8 cut(s) 91, 280, 627, 690, 694, 826, 976, 1179
BseMI GCAATG 3 cut(s) 474, 636, 660
BseMII CTCAG 1 cut(s) 779
BseNI ACTGG 5 cut(s) 853, 1080, 1162, 1185, 1190
BseRI GAGGAG 2 cut(s) 277, 911
BseXI GCAGC 4 cut(s) 488, 653, 1076, 1208
BsgI GTGCAG 2 cut(s) 50, 971
Bsh1236I CGCG 2 cut(s) 582, 1146
BshNI GGYRCC 1 cut(s) 60
BshTI ACCGGT 1 cut(s) 57
BsiSI CCGG 8 cut(s) 58, 206, 221, 302, 347, 533, 619, 1110
BslFI GGGAC 1 cut(s) 230
BsmAI GTCTC 1 cut(s) 114
BsmFI GGGAC 1 cut(s) 230
BsmI GAATGC 2 cut(s) 238, 469
Bso31I GGTCTC 1 cut(s) 114
Bsp13I TCCGGA 2 cut(s) 205, 346
Bsp1407I TGTACA 2 cut(s) 484, 1239
Bsp143I GATC 7 cut(s) 36, 421, 564, 702, 997, 1018, 1151
BspACI CCGC 5 cut(s) 42, 214, 416, 493, 1146
BspCNI CTCAG 1 cut(s) 778
BspEI TCCGGA 2 cut(s) 205, 346
BspFNI CGCG 2 cut(s) 582, 1146
BspLI GGNNCC 5 cut(s) 62, 218, 219, 351, 1153
BspMI ACCTGC 1 cut(s) 72
BspPI GGATC 4 cut(s) 429, 1005, 1146, 1159
BspT107I GGYRCC 1 cut(s) 60
BspTNI GGTCTC 1 cut(s) 114
BsrDI GCAATG 3 cut(s) 474, 636, 660
BsrFI RCCGGY 3 cut(s) 57, 301, 1109
BsrGI TGTACA 2 cut(s) 484, 1239
BsrI ACTGG 5 cut(s) 853, 1080, 1162, 1185, 1190
BssAI RCCGGY 3 cut(s) 57, 301, 1109
BssMI GATC 7 cut(s) 36, 421, 564, 702, 997, 1018, 1151
Bst4CI ACNGT 4 cut(s) 19, 71, 1204, 1211
Bst6I CTCTTC 3 cut(s) 254, 275, 974
BstAPI GCANNNNNTGC 2 cut(s) 473, 761
BstAUI TGTACA 2 cut(s) 484, 1239
BstBAI YACGTR 1 cut(s) 161
BstC8I GCNNGC 5 cut(s) 170, 414, 474, 590, 757
BstDEI CTNAG 4 cut(s) 765, 1043, 1118, 1230
BstF5I GGATG 8 cut(s) 91, 280, 627, 690, 694, 826, 976, 1179
BstFNI CGCG 2 cut(s) 582, 1146
BstHHI GCGC 1 cut(s) 412
BstKTI GATC 7 cut(s) 39, 424, 567, 705, 1000, 1021, 1154
BstMAI GTCTC 1 cut(s) 114
BstMBI GATC 7 cut(s) 36, 421, 564, 702, 997, 1018, 1151
BstMWI GCNNNNNNNGC 6 cut(s) 242, 464, 473, 650, 761, 1061
BstNSI RCATGY 2 cut(s) 112, 1053
BstSCI CCNGG 1 cut(s) 219
BstUI CGCG 2 cut(s) 582, 1146
BstV1I GCAGC 4 cut(s) 488, 653, 1076, 1208
BstV2I GAAGAC 1 cut(s) 1112
BstX2I RGATCY 3 cut(s) 421, 702, 1151
BstYI RGATCY 3 cut(s) 421, 702, 1151
BtsCI GGATG 8 cut(s) 91, 280, 627, 690, 694, 826, 976, 1179
BtsIMutI CAGTG 7 cut(s) 552, 773, 974, 1155, 1178, 1197, 1209
BveI ACCTGC 1 cut(s) 72
Cac8I GCNNGC 5 cut(s) 170, 414, 474, 590, 757
CfoI GCGC 1 cut(s) 412
Cfr10I RCCGGY 3 cut(s) 57, 301, 1109
Cfr13I GGNCC 1 cut(s) 217
CseI GACGC 1 cut(s) 1152
Csp6I GTAC 4 cut(s) 61, 485, 1123, 1240
CspAI ACCGGT 1 cut(s) 57
CviAII CATG 8 cut(s) 34, 109, 238, 721, 748, 808, 1035, 1050
CviQI GTAC 4 cut(s) 61, 485, 1123, 1240
DdeI CTNAG 4 cut(s) 765, 1043, 1118, 1230
DpnI GATC 7 cut(s) 38, 423, 566, 704, 999, 1020, 1153
DpnII GATC 7 cut(s) 36, 421, 564, 702, 997, 1018, 1151
Eam1104I CTCTTC 3 cut(s) 254, 275, 974
EarI CTCTTC 3 cut(s) 254, 275, 974
EciI GGCGGA 2 cut(s) 31, 431
Eco31I GGTCTC 1 cut(s) 114
Eco32I GATATC 1 cut(s) 660
Eco47I GGWCC 1 cut(s) 217
Eco57I CTGAAG 1 cut(s) 998
EcoO109I RGGNCCY 1 cut(s) 217
EcoRI GAATTC 1 cut(s) 928
EcoRV GATATC 1 cut(s) 660
FaeI CATG 8 cut(s) 37, 112, 241, 724, 751, 811, 1038, 1053
FaqI GGGAC 1 cut(s) 230
FatI CATG 8 cut(s) 33, 108, 237, 720, 747, 807, 1034, 1049
FauI CCCGC 1 cut(s) 207
FbaI TGATCA 2 cut(s) 564, 1018
Fnu4HI GCNGC 4 cut(s) 477, 642, 1065, 1197
FokI GGATG 8 cut(s) 98, 287, 634, 697, 701, 833, 983, 1186
Fsp4HI GCNGC 4 cut(s) 477, 642, 1065, 1197
FspI TGCGCA 1 cut(s) 411
GlaI GCGC 1 cut(s) 411
GluI GCNGC 4 cut(s) 477, 642, 1065, 1197
HapII CCGG 8 cut(s) 58, 206, 221, 302, 347, 533, 619, 1110
HgaI GACGC 1 cut(s) 1152
HhaI GCGC 1 cut(s) 412
Hin1II CATG 8 cut(s) 37, 112, 241, 724, 751, 811, 1038, 1053
Hin6I GCGC 1 cut(s) 410
HinP1I GCGC 1 cut(s) 410
HindIII AAGCTT 1 cut(s) 590
HinfI GANTC 3 cut(s) 4, 343, 542
HpaII CCGG 8 cut(s) 58, 206, 221, 302, 347, 533, 619, 1110
HphI GGTGA 3 cut(s) 320, 382, 1082
Hpy166II GTNNAC 3 cut(s) 106, 555, 1207
Hpy188I TCNGA 4 cut(s) 702, 714, 803, 843
Hpy188III TCNNGA 6 cut(s) 206, 347, 663, 968, 1001, 1171
Hpy8I GTNNAC 3 cut(s) 106, 555, 1207
HpyAV CCTTC 3 cut(s) 675, 998, 1074
HpyCH4III ACNGT 4 cut(s) 19, 71, 1204, 1211
HpyCH4IV ACGT 1 cut(s) 160
HpyF10VI GCNNNNNNNGC 6 cut(s) 242, 464, 473, 650, 761, 1061
HpyF3I CTNAG 4 cut(s) 765, 1043, 1118, 1230
HpySE526I ACGT 1 cut(s) 160
Hsp92II CATG 8 cut(s) 37, 112, 241, 724, 751, 811, 1038, 1053
HspAI GCGC 1 cut(s) 410
KflI GGGWCCC 1 cut(s) 217
Kpn2I TCCGGA 2 cut(s) 205, 346
KpnI GGTACC 1 cut(s) 64
Ksp22I TGATCA 2 cut(s) 564, 1018
Kzo9I GATC 7 cut(s) 36, 421, 564, 702, 997, 1018, 1151
LmnI GCTCC 2 cut(s) 349, 1226
Lsp1109I GCAGC 4 cut(s) 488, 653, 1076, 1208
LweI GCATC 5 cut(s) 76, 265, 426, 612, 961
MaeII ACGT 1 cut(s) 160
MaeIII GTNAC 4 cut(s) 19, 161, 370, 1176
MalI GATC 7 cut(s) 38, 423, 566, 704, 999, 1020, 1153
MboI GATC 7 cut(s) 36, 421, 564, 702, 997, 1018, 1151
MflI RGATCY 3 cut(s) 421, 702, 1151
MluCI AATT 3 cut(s) 498, 928, 936
MmeI TCCRAC 3 cut(s) 209, 294, 719
MnlI CCTC 8 cut(s) 76, 255, 258, 265, 412, 889, 1005, 1142
MroI TCCGGA 2 cut(s) 205, 346
MspA1I CMGCKG 1 cut(s) 190
MspI CCGG 8 cut(s) 58, 206, 221, 302, 347, 533, 619, 1110
MspR9I CCNGG 1 cut(s) 221
Mva1269I GAATGC 2 cut(s) 238, 469
MvnI CGCG 2 cut(s) 582, 1146
MwoI GCNNNNNNNGC 6 cut(s) 242, 464, 473, 650, 761, 1061
NciI CCSGG 1 cut(s) 221
NdeII GATC 7 cut(s) 36, 421, 564, 702, 997, 1018, 1151
NlaIII CATG 8 cut(s) 37, 112, 241, 724, 751, 811, 1038, 1053
NlaIV GGNNCC 5 cut(s) 62, 218, 219, 351, 1153
NmeAIII GCCGAG 1 cut(s) 465
NmuCI GTSAC 3 cut(s) 19, 370, 1176
NsbI TGCGCA 1 cut(s) 411
NspI RCATGY 2 cut(s) 112, 1053
PctI GAATGC 2 cut(s) 238, 469
PfeI GAWTC 2 cut(s) 343, 542
PinAI ACCGGT 1 cut(s) 57
PkrI GCNGC 4 cut(s) 478, 643, 1066, 1198
Ppu21I YACGTR 1 cut(s) 161
PpuMI RGGWCCY 1 cut(s) 217
Psp5II RGGWCCY 1 cut(s) 217
PspN4I GGNNCC 5 cut(s) 62, 218, 219, 351, 1153
PspPI GGNCC 1 cut(s) 217
PspPPI RGGWCCY 1 cut(s) 217
PsuI RGATCY 3 cut(s) 421, 702, 1151
PvuII CAGCTG 1 cut(s) 190
RsaI GTAC 4 cut(s) 62, 486, 1124, 1241
RsaNI GTAC 4 cut(s) 61, 485, 1123, 1240
SatI GCNGC 4 cut(s) 477, 642, 1065, 1197
Sau3AI GATC 7 cut(s) 36, 421, 564, 702, 997, 1018, 1151
Sau96I GGNCC 1 cut(s) 217
ScrFI CCNGG 1 cut(s) 221
SfaNI GCATC 5 cut(s) 76, 265, 426, 612, 961
SinI GGWCC 1 cut(s) 217
SmlI CTYRAG 1 cut(s) 858
SmoI CTYRAG 1 cut(s) 858
Sse9I AATT 3 cut(s) 498, 928, 936
SsiI CCGC 5 cut(s) 42, 214, 416, 493, 1146
SspI AATATT 1 cut(s) 519
StyD4I CCNGG 1 cut(s) 219
TaaI ACNGT 4 cut(s) 19, 71, 1204, 1211
TaiI ACGT 1 cut(s) 163
TasI AATT 3 cut(s) 498, 928, 936
TatI WGTACW 2 cut(s) 484, 1239
TfiI GAWTC 2 cut(s) 343, 542
TscAI CASTG 7 cut(s) 552, 773, 981, 1162, 1185, 1197, 1209
TseFI GTSAC 3 cut(s) 19, 370, 1176
TseI GCWGC 4 cut(s) 476, 641, 1064, 1196
Tsp45I GTSAC 3 cut(s) 19, 370, 1176
TspDTI ATGAA 3 cut(s) 309, 705, 1023
TspRI CASTG 7 cut(s) 552, 773, 981, 1162, 1185, 1197, 1209
VpaK11BI GGWCC 1 cut(s) 217
XapI RAATTY 1 cut(s) 928
XceI RCATGY 2 cut(s) 112, 1053
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.