MD09G1137200.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0006945
Physical Location & Seq
Reverse (-)
10628855 .. 10631094
2240 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1137200.v1.1.491

Sequence Viewer

Length: 2220 bp
ATGAGGCTGAGATCAATAGGTGAAAAGGGGACCCAAGTCTTCAACCAAAACCTGAAAATTACCCGGTTGGGGAAGTCGGGTCGGATCGACCAAGCGATTAAGGTTTTCTCACAAATGCCTCAAAGGAACACTGTAACATACAATTCCATGATCTCTGCCTATGCTAAGAATGGTAGAATTGGTAATGCACGCCAACTGTTCGATAAAATGCCGCACAGAAACTTGGTTTCTTGGAACACTATGATTTCCGGGTACCTGCACAATGACAGGGTTGAAGAAGCGTATAGGATTTTTGTTGACATGCCGGAGAGAGACCTTTACTCTTGGACTTTGATGATAACTTGCTTTACTCGTGGCGGTGAACTCGAGAGAGCCAGAAAGCTGTTCGATTTGCTTCCGGATAAGGGGGATGCTGCTTGTTGGAATGCAATGATTGCAGGGTATGCGAAGAAGGGGCAGTTTGACGATGCTAAGAGATTGTTTGATGAAATGCCGGAAAAAAATTTAGTTTCGTGGAATTCAATGCTTGCTGGGTATACGAAGAATGGGGAGATGCAGTTGGGAGTAAAAATTTTTGAAGAGATGCCTGAGAGGAATGTGGTTTCGTGGAATTTGATGTTGGATGGATTTTTTGAGGTTGGTGACTTGGATTCTGCTTGGAAGTTCTTTAAGAAGATTCCGGACCCGAATGTGGTTTCTTGGGTCACGATGTTATGTGGGTTTGCGCAAAATGGGGAGATCGCAAAGGCAGAGGATCTCTTTGAGCAGATGCCAAGTAGAAATGTGGTTTCTTGGAATGCAATGCTTGGAGCCTATGTTCGAGACCACCAAATTGACAAGGCTGTTCAATTATTCGGGGATATGCCTGAGAGGGATTCTGTATCGTGGACAACAATGATCAATGGGTATGTTCGTGTTGGTAAGCTTGACGAAGCAAGGCAATTGCTCAACCAGATGCCATACAAGAATATTGCTGCACAAACAGCAATGATGTCTGGATACTTGCAAAATGGAAGGATGGATGAAGCAAGTGAGATCTTCAATCCCATTTCCATCCGGGACGTTGTTTGTTGGAACACAATGATTGCAGGCTATGCTCAGTGTGGGAAAATGGTTGAAGCTCTGTCTCTTTTTAGAAAAATGATTAACAAGGACATTGTTTCTTGGAATACTATGATTACTGGTTATGCTCAAATAGGGCAGATGGAAAAAGCACTTGAAATCTTTGAGTCGATGGGTGAGAGGAGCATAGTTTCGTGGAATTCTCTGGTTACAGGTTACGTGCAAAACGGGTTATATCTGGATGCACTACGGAGGATCGTGAAGATGGGACAGGAAGGAAAGAGGCCTGATGAGTCGACTTTTGCTTGTGGTCTAAGTGCGTGCGCCAATCTTGCGGCTTTACAAGTTGGAAAGCAACTTCACCATTTGGTTGTGAAGTGTGGTTATGTAAATGACTTGTTCGTCAGCAATTCCCTGATTACCATGTATGCTAAATGCGGGAGAGTTGTGGATGCTAATCTTGTGTTCGAAGATATCAAGCGCGGTGATATTGTTTCTTGGAATTCTTTGATATCTGGGTATGCTTTAAACGGAGATGGTGAAGAGGCAGTAAAGCTCTTCAAAAATATGTTAATAGAAGGGGTGAATCCTGATCAGGTGACCTTTGTTGGGGTGTTATCGGCATGCAGCCACAGTGGGTTGGTTGAGTGCGGTTTAGAAATTTTTAAGAGCATGACTGAAGTTTACCTTATTGAACCTCTCGCCGAACACTATGCTTGCATGGTTGACCTGCTCGGTCGTGCAGGGAGGCTAGAGGAAGCCTTTGAAATGGTGAGGAATATGAAGATCATGACAACTGCTAAAATATGGGGTGCATTACTTGGAGCTTGTAGGATACACCGGAATCTACAACTTGGAAAGTATGCTTCTGAGAAGCTTTTAGAACTTGAACCTAATAAAGCTTCAAATTATGTGCTCTTGTCCAACATGCATGCTGAGGCCAGCAGATGGGACGAGGTTGAAAGAGTCAGGGTGTTAATGAAAGAAAGTAGAACGGGGAAGCAGCCAGGCTGCAGTTGGATTGAAATCAGAAATCACGTACATGCTTTTCTCTTTGATGATCTGGCGCAGCCTAGAACAGCAGAGCTTTCAAGCGTATTGAAGAGCCTAACCACAGAGATGAGAAACACAAGTTACATATCGACACCTTATGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR

Protein Analysis

740

Amino Acids

83.59

Weight (kDa)

6.64

Isoelectric Point (pI)

38.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_1 PF12854 42 - 70 1.2e-08 PPR repeat
PPR_2 PF13041 42 - 74 3.2e-08 PPR repeat family
PPR PF01535 45 - 74 1.5e-08 PPR repeat
PPR_2 PF13041 74 - 105 1.2e-07 PPR repeat family
PPR PF01535 76 - 105 2.1e-07 PPR repeat
PPR_1 PF12854 137 - 164 6.7e-06 PPR repeat
PPR_2 PF13041 137 - 169 4e-08 PPR repeat family
PPR PF01535 140 - 168 5.8e-09 PPR repeat
PPR_2 PF13041 168 - 200 1.1e-06 PPR repeat family
PPR PF01535 170 - 200 3.1e-07 PPR repeat
PPR_2 PF13041 229 - 262 3.1e-06 PPR repeat family
PPR PF01535 232 - 262 1e-05 PPR repeat
PPR_2 PF13041 260 - 291 5.9e-07 PPR repeat family
PPR_1 PF12854 291 - 319 4.3e-07 PPR repeat
PPR_2 PF13041 292 - 324 7e-08 PPR repeat family
PPR PF01535 294 - 323 6.5e-08 PPR repeat
PPR_2 PF13041 353 - 385 4.8e-08 PPR repeat family
PPR_1 PF12854 354 - 381 9.4e-07 PPR repeat
PPR PF01535 356 - 385 3.2e-09 PPR repeat
PPR_2 PF13041 384 - 417 2.6e-08 PPR repeat family
PPR PF01535 387 - 416 1e-08 PPR repeat
PPR_2 PF13041 416 - 464 3.7e-06 PPR repeat family
PPR_2 PF13041 517 - 564 2.2e-12 PPR repeat family
PPR PF01535 519 - 549 1.3e-08 PPR repeat
E_motif PF20431 634 - 696 1.4e-22 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1463
Acc16I TGCGCA 1 cut(s) 726
Acc36I ACCTGC 2 cut(s) 264, 1800
Acc65I GGTACC 1 cut(s) 252
AccB1I GGYRCC 1 cut(s) 252
AccB7I CCANNNNNTGG 1 cut(s) 2010
AccI GTMKAC 2 cut(s) 536, 1358
AccII CGCG 1 cut(s) 1545
AccIII TCCGGA 2 cut(s) 397, 679
AciI CCGC 6 cut(s) 212, 357, 1397, 1500, 1545, 1713
AclWI GGATC 3 cut(s) 92, 762, 1325
AcsI RAATTY 7 cut(s) 502, 517, 570, 610, 1261, 1564, 1722
AcuI CTGAAG 1 cut(s) 1761
AfaI GTAC 2 cut(s) 254, 2103
AfiI CCNNNNNNNGG 5 cut(s) 69, 404, 691, 1671, 2010
AjnI CCWGG 1 cut(s) 2068
AjuI GAANNNNNNNTTGG 2 cut(s) 602, 634
AloI GAACNNNNNNTCC 2 cut(s) 801, 833
AluBI AGCT 8 cut(s) 382, 925, 1121, 1618, 1889, 1939, 1964, 2149
AluI AGCT 8 cut(s) 382, 925, 1121, 1618, 1889, 1939, 1964, 2149
Alw21I GWGCWC 1 cut(s) 1980
Alw26I GTCTC 3 cut(s) 306, 816, 1131
AlwI GGATC 3 cut(s) 92, 762, 1325
Ama87I CYCGRG 1 cut(s) 365
Aor13HI TCCGGA 2 cut(s) 397, 679
AoxI GGCC 2 cut(s) 1346, 2001
ApeKI GCWGC 6 cut(s) 413, 974, 1689, 2065, 2073, 2131
ApoI RAATTY 7 cut(s) 502, 517, 570, 610, 1261, 1564, 1722
Asp718I GGTACC 1 cut(s) 252
AspLEI GCGC 4 cut(s) 727, 1388, 1545, 2131
AspS9I GGNCC 2 cut(s) 30, 682
AsuC2I CCSGG 3 cut(s) 64, 250, 1058
AsuII TTCGAA 1 cut(s) 1530
AvaI CYCGRG 1 cut(s) 365
AvaII GGWCC 2 cut(s) 30, 682
BanI GGYRCC 1 cut(s) 252
BauI CACGAG 1 cut(s) 351
BbsI GAAGAC 1 cut(s) 31
Bbv12I GWGCWC 1 cut(s) 1980
BbvCI CCTCAGC 1 cut(s) 1998
BbvI GCAGC 6 cut(s) 400, 961, 1701, 2060, 2077, 2143
BccI CCATC 8 cut(s) 617, 1012, 1061, 1198, 1228, 1321, 1592, 2004
BcgI CGANNNNNNTGC 2 cut(s) 1757, 1791
BciT130I CCWGG 1 cut(s) 2070
BciVI GTATCC 2 cut(s) 992, 1890
BclI TGATCA 2 cut(s) 897, 1654
BcnI CCSGG 3 cut(s) 64, 250, 1058
BcoDI GTCTC 3 cut(s) 306, 816, 1131
BfaI CTAG 2 cut(s) 1814, 2136
BfmI CTRYAG 1 cut(s) 2074
BfuAI ACCTGC 2 cut(s) 264, 1800
BfuI GTATCC 2 cut(s) 992, 1890
BglII AGATCT 1 cut(s) 1035
BisI GCNGC 8 cut(s) 212, 414, 975, 1398, 1690, 2066, 2074, 2132
BlsI GCNGC 8 cut(s) 213, 415, 976, 1399, 1691, 2067, 2075, 2133
Bme1390I CCNGG 4 cut(s) 64, 250, 1058, 2070
Bme18I GGWCC 2 cut(s) 30, 682
BmeT110I CYCGRG 1 cut(s) 365
BmgT120I GGNCC 2 cut(s) 30, 682
BmiI GGNNCC 5 cut(s) 31, 32, 254, 684, 811
BmrFI CCNGG 4 cut(s) 64, 250, 1058, 2070
BmsI GCATC 8 cut(s) 400, 457, 543, 573, 759, 945, 1294, 1504
BoxI GACNNNNGTC 1 cut(s) 35
BpiI GAAGAC 1 cut(s) 31
Bpu10I CCTNAGC 1 cut(s) 1998
Bpu14I TTCGAA 1 cut(s) 1530
BpuMI CCSGG 3 cut(s) 64, 250, 1058
BsaAI YACGTR 2 cut(s) 1282, 2101
BsaBI GATNNNNATC 2 cut(s) 1518, 2087
BsaI GGTCTC 2 cut(s) 306, 816
BsaWI WCCGGW 3 cut(s) 397, 679, 1902
BsaXI ACNNNNNCTCC 4 cut(s) 542, 572, 801, 831
Bsc4I CCNNNNNNNGG 5 cut(s) 69, 404, 691, 1671, 2010
Bse1I ACTGG 1 cut(s) 1186
Bse3DI GCAATG 3 cut(s) 435, 807, 993
Bse8I GATNNNNATC 2 cut(s) 1518, 2087
BseAI TCCGGA 2 cut(s) 397, 679
BseBI CCWGG 1 cut(s) 2070
BseGI GGATG 7 cut(s) 415, 628, 1023, 1027, 1053, 1309, 1519
BseJI GATNNNNATC 2 cut(s) 1518, 2087
BseLI CCNNNNNNNGG 5 cut(s) 69, 404, 691, 1671, 2010
BseMI GCAATG 3 cut(s) 435, 807, 993
BseMII CTCAG 5 cut(s) 579, 858, 1112, 1923, 1989
BseNI ACTGG 1 cut(s) 1186
BseRI GAGGAG 1 cut(s) 1258
BseXI GCAGC 6 cut(s) 400, 961, 1701, 2060, 2077, 2143
BseYI CCCAGC 1 cut(s) 530
BsgI GTGCAG 3 cut(s) 242, 960, 1824
Bsh1236I CGCG 1 cut(s) 1545
Bsh1285I CGRYCG 1 cut(s) 1801
BshFI GGCC 2 cut(s) 1348, 2003
BshNI GGYRCC 1 cut(s) 252
BsiEI CGRYCG 1 cut(s) 1801
BsiHKAI GWGCWC 1 cut(s) 1980
BsiHKCI CYCGRG 1 cut(s) 365
BsiSI CCGG 8 cut(s) 64, 249, 305, 398, 494, 680, 1057, 1903
BslFI GGGAC 4 cut(s) 43, 1073, 1344, 2027
BslI CCNNNNNNNGG 5 cut(s) 69, 404, 691, 1671, 2010
BsmAI GTCTC 3 cut(s) 306, 816, 1131
BsmFI GGGAC 4 cut(s) 43, 1073, 1344, 2027
BsmI GAATGC 2 cut(s) 430, 802
BsnI GGCC 2 cut(s) 1348, 2003
Bso31I GGTCTC 2 cut(s) 306, 816
BsoBI CYCGRG 1 cut(s) 365
Bsp119I TTCGAA 1 cut(s) 1530
Bsp1286I GDGCHC 1 cut(s) 1980
Bsp13I TCCGGA 2 cut(s) 397, 679
BspACI CCGC 6 cut(s) 212, 357, 1397, 1500, 1545, 1713
BspANI GGCC 2 cut(s) 1348, 2003
BspCNI CTCAG 5 cut(s) 580, 859, 1111, 1924, 1990
BspEI TCCGGA 2 cut(s) 397, 679
BspFNI CGCG 1 cut(s) 1545
BspHI TCATGA 1 cut(s) 1851
BspLI GGNNCC 5 cut(s) 31, 32, 254, 684, 811
BspMAI CTGCAG 1 cut(s) 2078
BspMI ACCTGC 2 cut(s) 264, 1800
BspPI GGATC 3 cut(s) 92, 762, 1325
BspQI GCTCTTC 2 cut(s) 1625, 2159
BspT104I TTCGAA 1 cut(s) 1530
BspT107I GGYRCC 1 cut(s) 252
BspTNI GGTCTC 2 cut(s) 306, 816
BsrDI GCAATG 3 cut(s) 435, 807, 993
BsrI ACTGG 1 cut(s) 1186
BssNAI GTATAC 1 cut(s) 537
BssSI CACGAG 1 cut(s) 351
Bst1107I GTATAC 1 cut(s) 537
Bst2BI CACGAG 1 cut(s) 351
Bst2UI CCWGG 1 cut(s) 2070
Bst4CI ACNGT 3 cut(s) 133, 198, 1697
Bst6I CTCTTC 4 cut(s) 573, 1599, 1625, 2159
BstAPI GCANNNNNTGC 3 cut(s) 434, 443, 1094
BstBAI YACGTR 2 cut(s) 1282, 2101
BstBI TTCGAA 1 cut(s) 1530
BstC8I GCNNGC 8 cut(s) 190, 528, 1090, 1384, 1687, 1780, 1995, 2005
BstDEI CTNAG 9 cut(s) 8, 165, 471, 588, 867, 1098, 1376, 1932, 1998
BstEII GGTNACC 1 cut(s) 1660
BstF5I GGATG 7 cut(s) 415, 628, 1023, 1027, 1053, 1309, 1519
BstFNI CGCG 1 cut(s) 1545
BstHHI GCGC 4 cut(s) 727, 1388, 1545, 2131
BstMAI GTCTC 3 cut(s) 306, 816, 1131
BstMCI CGRYCG 1 cut(s) 1801
BstMWI GCNNNNNNNGC 5 cut(s) 434, 443, 983, 1094, 1394
BstNI CCWGG 1 cut(s) 2070
BstNSI RCATGY 5 cut(s) 304, 1689, 1993, 1997, 2108
BstPAI GACNNNNGTC 1 cut(s) 35
BstPI GGTNACC 1 cut(s) 1660
BstSCI CCNGG 4 cut(s) 62, 248, 1056, 2068
BstSFI CTRYAG 1 cut(s) 2074
BstUI CGCG 1 cut(s) 1545
BstV1I GCAGC 6 cut(s) 400, 961, 1701, 2060, 2077, 2143
BstV2I GAAGAC 1 cut(s) 31
BstX2I RGATCY 2 cut(s) 754, 1035
BstYI RGATCY 2 cut(s) 754, 1035
BstZ17I GTATAC 1 cut(s) 537
BsuI GTATCC 2 cut(s) 992, 1890
BsuRI GGCC 2 cut(s) 1348, 2003
BtsCI GGATG 7 cut(s) 415, 628, 1023, 1027, 1053, 1309, 1519
BtsIMutI CAGTG 3 cut(s) 129, 1106, 1702
BveI ACCTGC 2 cut(s) 264, 1800
Cac8I GCNNGC 8 cut(s) 190, 528, 1090, 1384, 1687, 1780, 1995, 2005
CciI TCATGA 1 cut(s) 1851
CfoI GCGC 4 cut(s) 727, 1388, 1545, 2131
Cfr13I GGNCC 2 cut(s) 30, 682
Csp6I GTAC 2 cut(s) 253, 2102
CviQI GTAC 2 cut(s) 253, 2102
DdeI CTNAG 9 cut(s) 8, 165, 471, 588, 867, 1098, 1376, 1932, 1998
DraI TTTAAA 1 cut(s) 1590
DrdI GACNNNNNNGTC 1 cut(s) 1463
DseDI GACNNNNNNGTC 1 cut(s) 1463
Eam1104I CTCTTC 4 cut(s) 573, 1599, 1625, 2159
EarI CTCTTC 4 cut(s) 573, 1599, 1625, 2159
Eco147I AGGCCT 1 cut(s) 1348
Eco31I GGTCTC 2 cut(s) 306, 816
Eco32I GATATC 2 cut(s) 1537, 1575
Eco47I GGWCC 2 cut(s) 30, 682
Eco57I CTGAAG 1 cut(s) 1761
Eco88I CYCGRG 1 cut(s) 365
Eco91I GGTNACC 1 cut(s) 1660
EcoO109I RGGNCCY 1 cut(s) 30
EcoO65I GGTNACC 1 cut(s) 1660
EcoRI GAATTC 3 cut(s) 517, 1261, 1564
EcoRII CCWGG 1 cut(s) 2068
EcoRV GATATC 2 cut(s) 1537, 1575
EcoT22I ATGCAT 1 cut(s) 1995
FalI AAGNNNNNCTT 2 cut(s) 1734, 1766
FaqI GGGAC 4 cut(s) 43, 1073, 1344, 2027
FauI CCCGC 1 cut(s) 1493
FbaI TGATCA 2 cut(s) 897, 1654
FblI GTMKAC 2 cut(s) 536, 1358
Fnu4HI GCNGC 8 cut(s) 212, 414, 975, 1398, 1690, 2066, 2074, 2132
FokI GGATG 7 cut(s) 422, 635, 1030, 1034, 1040, 1316, 1526
Fsp4HI GCNGC 8 cut(s) 212, 414, 975, 1398, 1690, 2066, 2074, 2132
FspBI CTAG 2 cut(s) 1814, 2136
FspI TGCGCA 1 cut(s) 726
GlaI GCGC 4 cut(s) 726, 1387, 1544, 2130
GluI GCNGC 8 cut(s) 212, 414, 975, 1398, 1690, 2066, 2074, 2132
GsaI CCCAGC 1 cut(s) 534
HaeIII GGCC 2 cut(s) 1348, 2003
HapII CCGG 8 cut(s) 64, 249, 305, 398, 494, 680, 1057, 1903
HhaI GCGC 4 cut(s) 727, 1388, 1545, 2131
Hin6I GCGC 4 cut(s) 725, 1386, 1543, 2129
HinP1I GCGC 4 cut(s) 725, 1386, 1543, 2129
HincII GTYRAC 3 cut(s) 298, 1359, 1789
HindII GTYRAC 3 cut(s) 298, 1359, 1789
HindIII AAGCTT 3 cut(s) 923, 1937, 1962
HinfI GANTC 8 cut(s) 650, 676, 875, 1229, 1355, 1648, 1906, 2028
HpaII CCGG 8 cut(s) 64, 249, 305, 398, 494, 680, 1057, 1903
Hpy166II GTNNAC 7 cut(s) 298, 362, 537, 888, 1359, 1747, 1789
Hpy188I TCNGA 3 cut(s) 84, 1933, 2093
Hpy8I GTNNAC 7 cut(s) 298, 362, 537, 888, 1359, 1747, 1789
HpyAV CCTTC 4 cut(s) 445, 1008, 1331, 1634
HpyCH4III ACNGT 3 cut(s) 133, 198, 1697
HpyCH4IV ACGT 3 cut(s) 1062, 1281, 2100
HpyF10VI GCNNNNNNNGC 5 cut(s) 434, 443, 983, 1094, 1394
HpyF3I CTNAG 9 cut(s) 8, 165, 471, 588, 867, 1098, 1376, 1932, 1998
HpySE526I ACGT 3 cut(s) 1062, 1281, 2100
HspAI GCGC 4 cut(s) 725, 1386, 1543, 2129
KflI GGGWCCC 1 cut(s) 30
Kpn2I TCCGGA 2 cut(s) 397, 679
KpnI GGTACC 1 cut(s) 256
Ksp22I TGATCA 2 cut(s) 897, 1654
LguI GCTCTTC 2 cut(s) 1625, 2159
LmnI GCTCC 3 cut(s) 809, 1245, 1886
Lsp1109I GCAGC 6 cut(s) 400, 961, 1701, 2060, 2077, 2143
LweI GCATC 8 cut(s) 400, 457, 543, 573, 759, 945, 1294, 1504
MaeI CTAG 2 cut(s) 1814, 2136
MaeII ACGT 3 cut(s) 1062, 1281, 2100
MaeIII GTNAC 7 cut(s) 133, 641, 703, 1270, 1277, 1660, 2195
MfeI CAATTG 1 cut(s) 941
MflI RGATCY 2 cut(s) 754, 1035
MhlI GDGCHC 1 cut(s) 1980
MlyI GAGTC 3 cut(s) 1238, 1364, 2037
MmeI TCCRAC 7 cut(s) 62, 401, 600, 1052, 1390, 2010, 2060
Mph1103I ATGCAT 1 cut(s) 1995
MroI TCCGGA 2 cut(s) 397, 679
MseI TTAA 7 cut(s) 99, 669, 1146, 1589, 1634, 1728, 2039
MslI CAYNNNNRTG 2 cut(s) 2103, 2180
MspI CCGG 8 cut(s) 64, 249, 305, 398, 494, 680, 1057, 1903
MspR9I CCNGG 4 cut(s) 64, 250, 1058, 2070
MunI CAATTG 1 cut(s) 941
Mva1269I GAATGC 2 cut(s) 430, 802
MvaI CCWGG 1 cut(s) 2070
MvnI CGCG 1 cut(s) 1545
MwoI GCNNNNNNNGC 5 cut(s) 434, 443, 983, 1094, 1394
NciI CCSGG 3 cut(s) 64, 250, 1058
NlaIV GGNNCC 5 cut(s) 31, 32, 254, 684, 811
NmuCI GTSAC 3 cut(s) 641, 703, 1660
NsbI TGCGCA 1 cut(s) 726
NsiI ATGCAT 1 cut(s) 1995
NspI RCATGY 5 cut(s) 304, 1689, 1993, 1997, 2108
NspV TTCGAA 1 cut(s) 1530
PaeI GCATGC 2 cut(s) 1689, 1997
PaeR7I CTCGAG 1 cut(s) 365
PagI TCATGA 1 cut(s) 1851
PceI AGGCCT 1 cut(s) 1348
PciSI GCTCTTC 2 cut(s) 1625, 2159
PctI GAATGC 2 cut(s) 430, 802
PfeI GAWTC 5 cut(s) 650, 676, 875, 1648, 1906
PflMI CCANNNNNTGG 1 cut(s) 2010
PfoI TCCNGGA 1 cut(s) 1056
PkrI GCNGC 8 cut(s) 213, 415, 976, 1399, 1691, 2067, 2075, 2133
PleI GAGTC 3 cut(s) 1237, 1363, 2036
PpsI GAGTC 3 cut(s) 1237, 1363, 2036
Ppu21I YACGTR 2 cut(s) 1282, 2101
PpuMI RGGWCCY 1 cut(s) 30
PshAI GACNNNNGTC 1 cut(s) 35
Psp5II RGGWCCY 1 cut(s) 30
Psp6I CCWGG 1 cut(s) 2068
PspEI GGTNACC 1 cut(s) 1660
PspFI CCCAGC 1 cut(s) 530
PspGI CCWGG 1 cut(s) 2068
PspN4I GGNNCC 5 cut(s) 31, 32, 254, 684, 811
PspPI GGNCC 2 cut(s) 30, 682
PspPPI RGGWCCY 1 cut(s) 30
PstI CTGCAG 1 cut(s) 2078
PsuI RGATCY 2 cut(s) 754, 1035
RsaI GTAC 2 cut(s) 254, 2103
RsaNI GTAC 2 cut(s) 253, 2102
RseI CAYNNNNRTG 2 cut(s) 2103, 2180
SalI GTCGAC 1 cut(s) 1357
SapI GCTCTTC 2 cut(s) 1625, 2159
SaqAI TTAA 7 cut(s) 99, 669, 1146, 1589, 1634, 1728, 2039
SatI GCNGC 8 cut(s) 212, 414, 975, 1398, 1690, 2066, 2074, 2132
Sau96I GGNCC 2 cut(s) 30, 682
SchI GAGTC 3 cut(s) 1238, 1364, 2037
ScrFI CCNGG 4 cut(s) 64, 250, 1058, 2070
SduI GDGCHC 1 cut(s) 1980
SfaNI GCATC 8 cut(s) 400, 457, 543, 573, 759, 945, 1294, 1504
SfcI CTRYAG 1 cut(s) 2074
Sfr274I CTCGAG 1 cut(s) 365
SfuI TTCGAA 1 cut(s) 1530
SinI GGWCC 2 cut(s) 30, 682
SlaI CTCGAG 1 cut(s) 365
SmiMI CAYNNNNRTG 2 cut(s) 2103, 2180
SmlI CTYRAG 1 cut(s) 365
SmoI CTYRAG 1 cut(s) 365
SphI GCATGC 2 cut(s) 1689, 1997
SseBI AGGCCT 1 cut(s) 1348
SsiI CCGC 6 cut(s) 212, 357, 1397, 1500, 1545, 1713
SspI AATATT 1 cut(s) 970
SspMI CTAG 2 cut(s) 1814, 2136
StuI AGGCCT 1 cut(s) 1348
StyD4I CCNGG 4 cut(s) 62, 248, 1056, 2068
TaaI ACNGT 3 cut(s) 133, 198, 1697
TaiI ACGT 3 cut(s) 1065, 1284, 2103
TaqI TCGA 9 cut(s) 87, 201, 366, 387, 820, 1232, 1358, 1530, 2204
TaqII GACCGA 1 cut(s) 1787
TauI GCSGC 2 cut(s) 214, 1400
TfiI GAWTC 5 cut(s) 650, 676, 875, 1648, 1906
Tru1I TTAA 7 cut(s) 99, 669, 1146, 1589, 1634, 1728, 2039
Tru9I TTAA 7 cut(s) 99, 669, 1146, 1589, 1634, 1728, 2039
TscAI CASTG 3 cut(s) 136, 1106, 1702
TseFI GTSAC 3 cut(s) 641, 703, 1660
TseI GCWGC 6 cut(s) 413, 974, 1689, 2065, 2073, 2131
Tsp45I GTSAC 3 cut(s) 641, 703, 1660
TspDTI ATGAA 4 cut(s) 501, 1038, 1859, 2057
TspGWI ACGGA 2 cut(s) 1327, 1608
TspRI CASTG 3 cut(s) 136, 1106, 1702
Van91I CCANNNNNTGG 1 cut(s) 2010
VpaK11BI GGWCC 2 cut(s) 30, 682
XapI RAATTY 7 cut(s) 502, 517, 570, 610, 1261, 1564, 1722
XceI RCATGY 5 cut(s) 304, 1689, 1993, 1997, 2108
XcmI CCANNNNNNNNNTGG 1 cut(s) 2076
XhoI CTCGAG 1 cut(s) 365
XmiI GTMKAC 2 cut(s) 536, 1358
XspI CTAG 2 cut(s) 1814, 2136
Zsp2I ATGCAT 1 cut(s) 1995
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.