MD02G1166700.v1.1
MYB Family

Myb-like DNA-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0006004
Physical Location & Seq
Forward (+)
14302904 .. 14304995
2092 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1166700.v1.1.491

Sequence Viewer

Length: 1137 bp
ATGGAGCTGTTGAGCTTGGACTCCAGTCCTCTGTTCGTCCCGAAAACAATCACCGACTTTCTCGCCCAGCTCGCCGCTATTAAAGATGGCTCTCAGAGGTCTTCAGAGCTCGATGGGTACGTGAAGAGATTGGAAGATGAACTCAGAAAGATTGAAGTTTTCAAGCGGGAGCTTCCTCTCTGCATGCTTCTCTTGAAGGATGCGATTGAGCTGTTGAAGGAGGAAGGAATGCGGTGGAGGCGAATGGAAGAGCGGCCGGTGGTGGAGGTGTTCATACCGTTGAAGGGCAATTCTGAGGAGGACGGAGGGTTAGTTTCGGGAAAGGAAAATATTGACAAGAAGAACTGGATGAGCTCTGCTCAGCTGTGGAACACGAGCATCAACGTTTTCGATTACACCAAACGCGACTCTGTTGAACTTAAACTGGGGAATGAGGAAGATGATCGGTCGGTGACTGAGAACCCAATTGAGGTGAGTAATAACAGAGCCGTGGAGGGGGCATTACTGGCATTCAGGGGACAGAATGAGTTGTCTGGTTTTGCAAAACCATGTTTGAAGGAAGTCAAGGAGGTTTCCGAGGTTCCAAACCTTTGTCTTATGACTCCATTGATGTCTGAAGCACTGGGTTGTTCTGCAAGCGCAAACTCTAGGACCAGTAATGGCTGCAAAGGCAGCGGTTCGGGTTCTGGTGTGGCAGGACAAGTGAAATTACTGAACAAGCCCCAGCCACAGCAGCAGCAGCAACCCTTGAGGAAACCAAGGCGGTGCTGGTCCCCGGAGCTCCATCGCCGCTTTGTTGATGCCCTTCAGCAGCTTGGAGGAACAGAAGTAGCCACTCCTAAGCAGATAAGAGAGCTTATGCAGGTGGAGGGTCTCACCAATGATGAAGTTAAGAGCCATTTGCAGAAATACCGCCTTCACATCCGAAAGCTACCGCCTTCTGCTGCTGCCGGCCAAGGGAATGCCTTGCTCATGTCCTTGGATCACAGTGGAGACCATTCTAAGGCAAACAACGTACAGTCTGGTTCTCCACAGGGTCCTCTCCTTGCGGGCGGGTTTGCCAAATGTCGATCAACAACTAGAGGCGAGTCAGGCAACAGCAGGGAAGCCGAAGAGGACGAGAAATCGGATGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Myb_DNA-binding

Protein Analysis

379

Amino Acids

41.7

Weight (kDa)

6.1

Isoelectric Point (pI)

57.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HHO5_N PF26575 5 - 76 1.4e-30 HHO5-like, N-terminal domain
Myb_DNA-binding PF00249 254 - 305 2e-07 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 853
Acc36I ACCTGC 1 cut(s) 853
AccBSI CCGCTC 1 cut(s) 253
AccII CGCG 1 cut(s) 405
AclI AACGTT 1 cut(s) 384
AclWI GGATC 1 cut(s) 990
AcoI YGGCCR 2 cut(s) 254, 952
AcuI CTGAAG 3 cut(s) 87, 636, 791
AfaI GTAC 2 cut(s) 119, 1017
AfiI CCNNNNNNNGG 5 cut(s) 284, 469, 495, 957, 1003
AgsI TTSAA 7 cut(s) 155, 163, 196, 217, 283, 416, 556
Alw21I GWGCWC 3 cut(s) 111, 356, 783
Alw26I GTCTC 2 cut(s) 878, 987
AlwI GGATC 1 cut(s) 990
AoxI GGCC 2 cut(s) 254, 952
ApeKI GCWGC 8 cut(s) 663, 672, 733, 736, 739, 811, 944, 947
AspLEI GCGC 1 cut(s) 641
AspS9I GGNCC 3 cut(s) 651, 771, 1037
AsuC2I CCSGG 1 cut(s) 776
AsuHPI GGTGA 4 cut(s) 43, 463, 484, 868
AvaII GGWCC 3 cut(s) 651, 771, 1037
BanII GRGCYC 3 cut(s) 111, 356, 783
BauI CACGAG 1 cut(s) 373
BbsI GAAGAC 1 cut(s) 93
Bbv12I GWGCWC 3 cut(s) 111, 356, 783
BbvI GCAGC 8 cut(s) 650, 684, 745, 748, 751, 823, 931, 934
BccI CCATC 4 cut(s) 80, 107, 792, 1124
BceAI ACGGC 1 cut(s) 473
BcnI CCSGG 1 cut(s) 776
BcoDI GTCTC 2 cut(s) 878, 987
BfaI CTAG 2 cut(s) 648, 1080
BfuAI ACCTGC 1 cut(s) 853
BlpI GCTNAGC 1 cut(s) 360
Bme1390I CCNGG 1 cut(s) 776
Bme18I GGWCC 3 cut(s) 651, 771, 1037
BmgT120I GGNCC 3 cut(s) 651, 771, 1037
BmiI GGNNCC 3 cut(s) 582, 773, 1038
BmrFI CCNGG 1 cut(s) 776
BmrI ACTGGG 2 cut(s) 434, 632
BmsI GCATC 3 cut(s) 190, 387, 790
BmuI ACTGGG 2 cut(s) 434, 632
BoxI GACNNNNGTC 1 cut(s) 24
BpiI GAAGAC 1 cut(s) 93
BplI GAGNNNNNCTC 2 cut(s) 343, 375
BpmI CTGGAG 1 cut(s) 7
Bpu10I CCTNAGC 1 cut(s) 840
Bpu1102I GCTNAGC 1 cut(s) 360
BpuEI CTTGAG 1 cut(s) 769
BpuMI CCSGG 1 cut(s) 776
BsaAI YACGTR 1 cut(s) 121
BsaI GGTCTC 2 cut(s) 878, 987
BsaJI CCNNGG 6 cut(s) 489, 576, 758, 774, 955, 978
BsaXI ACNNNNNCTCC 2 cut(s) 297, 327
Bsc4I CCNNNNNNNGG 5 cut(s) 284, 469, 495, 957, 1003
Bse118I RCCGGY 2 cut(s) 256, 950
Bse1I ACTGG 6 cut(s) 24, 350, 429, 510, 627, 654
BseDI CCNNGG 6 cut(s) 489, 576, 758, 774, 955, 978
BseGI GGATG 4 cut(s) 205, 354, 921, 1135
BseLI CCNNNNNNNGG 5 cut(s) 284, 469, 495, 957, 1003
BseMII CTCAG 5 cut(s) 107, 157, 285, 374, 447
BseNI ACTGG 6 cut(s) 24, 350, 429, 510, 627, 654
BseRI GAGGAG 1 cut(s) 311
BseX3I CGGCCG 1 cut(s) 254
BseXI GCAGC 8 cut(s) 650, 684, 745, 748, 751, 823, 931, 934
BseYI CCCAGC 2 cut(s) 66, 723
Bsh1236I CGCG 1 cut(s) 405
Bsh1285I CGRYCG 2 cut(s) 257, 449
BshFI GGCC 2 cut(s) 256, 954
BsiEI CGRYCG 2 cut(s) 257, 449
BsiHKAI GWGCWC 3 cut(s) 111, 356, 783
BsiSI CCGG 3 cut(s) 257, 776, 951
BslFI GGGAC 3 cut(s) 23, 531, 757
BslI CCNNNNNNNGG 5 cut(s) 284, 469, 495, 957, 1003
BsmAI GTCTC 2 cut(s) 878, 987
BsmFI GGGAC 3 cut(s) 23, 531, 757
BsmI GAATGC 3 cut(s) 234, 509, 967
BsnI GGCC 2 cut(s) 256, 954
Bso31I GGTCTC 2 cut(s) 878, 987
Bsp1286I GDGCHC 3 cut(s) 111, 356, 783
Bsp143I GATC 3 cut(s) 442, 982, 1070
Bsp1720I GCTNAGC 1 cut(s) 360
BspANI GGCC 2 cut(s) 256, 954
BspCNI CTCAG 5 cut(s) 106, 156, 286, 373, 448
BspFNI CGCG 1 cut(s) 405
BspLI GGNNCC 3 cut(s) 582, 773, 1038
BspMI ACCTGC 1 cut(s) 853
BspPI GGATC 1 cut(s) 990
BspQI GCTCTTC 1 cut(s) 243
BspTNI GGTCTC 2 cut(s) 878, 987
BsrBI CCGCTC 1 cut(s) 253
BsrFI RCCGGY 2 cut(s) 256, 950
BsrI ACTGG 6 cut(s) 24, 350, 429, 510, 627, 654
BssAI RCCGGY 2 cut(s) 256, 950
BssECI CCNNGG 6 cut(s) 489, 576, 758, 774, 955, 978
BssMI GATC 3 cut(s) 442, 982, 1070
BssSI CACGAG 1 cut(s) 373
BssT1I CCWWGG 3 cut(s) 758, 955, 978
Bst2BI CACGAG 1 cut(s) 373
Bst4CI ACNGT 3 cut(s) 279, 989, 1020
Bst6I CTCTTC 3 cut(s) 119, 243, 1107
BstBAI YACGTR 1 cut(s) 121
BstC8I GCNNGC 5 cut(s) 72, 185, 637, 952, 1051
BstDEI CTNAG 7 cut(s) 93, 143, 294, 360, 456, 840, 1002
BstDSI CCRYGG 1 cut(s) 489
BstF5I GGATG 4 cut(s) 205, 354, 921, 1135
BstFNI CGCG 1 cut(s) 405
BstHHI GCGC 1 cut(s) 641
BstKTI GATC 3 cut(s) 445, 985, 1073
BstMAI GTCTC 2 cut(s) 878, 987
BstMBI GATC 3 cut(s) 442, 982, 1070
BstMCI CGRYCG 2 cut(s) 257, 449
BstMWI GCNNNNNNNGC 8 cut(s) 71, 238, 506, 669, 672, 733, 739, 1092
BstNSI RCATGY 1 cut(s) 187
BstPAI GACNNNNGTC 1 cut(s) 24
BstSCI CCNGG 1 cut(s) 774
BstUI CGCG 1 cut(s) 405
BstV1I GCAGC 8 cut(s) 650, 684, 745, 748, 751, 823, 931, 934
BstV2I GAAGAC 1 cut(s) 93
BstZI CGGCCG 1 cut(s) 254
BsuRI GGCC 2 cut(s) 256, 954
BtgI CCRYGG 1 cut(s) 489
BtgZI GCGATG 1 cut(s) 770
BtsCI GGATG 4 cut(s) 205, 354, 921, 1135
BtsIMutI CAGTG 2 cut(s) 620, 994
BveI ACCTGC 1 cut(s) 853
Cac8I GCNNGC 5 cut(s) 72, 185, 637, 952, 1051
CfoI GCGC 1 cut(s) 641
Cfr10I RCCGGY 2 cut(s) 256, 950
Cfr13I GGNCC 3 cut(s) 651, 771, 1037
Csp6I GTAC 2 cut(s) 118, 1016
CviAII CATG 3 cut(s) 184, 549, 973
CviQI GTAC 2 cut(s) 118, 1016
DdeI CTNAG 7 cut(s) 93, 143, 294, 360, 456, 840, 1002
DpnI GATC 3 cut(s) 444, 984, 1072
DpnII GATC 3 cut(s) 442, 982, 1070
EaeI YGGCCR 2 cut(s) 254, 952
EagI CGGCCG 1 cut(s) 254
Eam1104I CTCTTC 3 cut(s) 119, 243, 1107
EarI CTCTTC 3 cut(s) 119, 243, 1107
Ecl136II GAGCTC 3 cut(s) 109, 354, 781
EclXI CGGCCG 1 cut(s) 254
Eco130I CCWWGG 3 cut(s) 758, 955, 978
Eco24I GRGCYC 3 cut(s) 111, 356, 783
Eco31I GGTCTC 2 cut(s) 878, 987
Eco47I GGWCC 3 cut(s) 651, 771, 1037
Eco52I CGGCCG 1 cut(s) 254
Eco53kI GAGCTC 3 cut(s) 109, 354, 781
Eco57I CTGAAG 3 cut(s) 87, 636, 791
EcoICRI GAGCTC 3 cut(s) 109, 354, 781
EcoO109I RGGNCCY 1 cut(s) 1037
EcoT14I CCWWGG 3 cut(s) 758, 955, 978
EcoT38I GRGCYC 3 cut(s) 111, 356, 783
ErhI CCWWGG 3 cut(s) 758, 955, 978
FaeI CATG 3 cut(s) 187, 552, 976
FaiI YATR 6 cut(s) 185, 275, 550, 599, 860, 974
FaqI GGGAC 3 cut(s) 23, 531, 757
FatI CATG 3 cut(s) 183, 548, 972
FauI CCCGC 3 cut(s) 159, 1042, 1046
FokI GGATG 3 cut(s) 212, 361, 908
FriOI GRGCYC 3 cut(s) 111, 356, 783
FspBI CTAG 2 cut(s) 648, 1080
GlaI GCGC 1 cut(s) 640
GsaI CCCAGC 2 cut(s) 70, 727
GsuI CTGGAG 1 cut(s) 7
HaeIII GGCC 2 cut(s) 256, 954
HapII CCGG 3 cut(s) 257, 776, 951
HhaI GCGC 1 cut(s) 641
Hin1II CATG 3 cut(s) 187, 552, 976
Hin6I GCGC 1 cut(s) 639
HinP1I GCGC 1 cut(s) 639
HinfI GANTC 4 cut(s) 20, 407, 601, 1088
HpaII CCGG 3 cut(s) 257, 776, 951
HphI GGTGA 4 cut(s) 43, 463, 484, 868
Hpy188I TCNGA 8 cut(s) 96, 106, 146, 295, 577, 616, 926, 1129
Hpy188III TCNNGA 3 cut(s) 40, 193, 318
HpyAV CCTTC 8 cut(s) 190, 211, 218, 277, 550, 815, 926, 948
HpyCH4III ACNGT 3 cut(s) 279, 989, 1020
HpyCH4IV ACGT 3 cut(s) 120, 384, 1014
HpyCH4V TGCA 6 cut(s) 183, 542, 635, 666, 862, 904
HpyF10VI GCNNNNNNNGC 8 cut(s) 71, 238, 506, 669, 672, 733, 739, 1092
HpyF3I CTNAG 7 cut(s) 93, 143, 294, 360, 456, 840, 1002
HpySE526I ACGT 3 cut(s) 120, 384, 1014
Hsp92II CATG 3 cut(s) 187, 552, 976
HspAI GCGC 1 cut(s) 639
KroI GCCGGC 1 cut(s) 950
KroNI GCCGGC 1 cut(s) 952
Kzo9I GATC 3 cut(s) 442, 982, 1070
LguI GCTCTTC 1 cut(s) 243
LmnI GCTCC 4 cut(s) 4, 169, 778, 786
Lsp1109I GCAGC 8 cut(s) 650, 684, 745, 748, 751, 823, 931, 934
LweI GCATC 3 cut(s) 190, 387, 790
MaeI CTAG 2 cut(s) 648, 1080
MaeII ACGT 3 cut(s) 120, 384, 1014
MaeIII GTNAC 1 cut(s) 451
MalI GATC 3 cut(s) 444, 984, 1072
MbiI CCGCTC 1 cut(s) 253
MboI GATC 3 cut(s) 442, 982, 1070
MboII GAAGA 7 cut(s) 93, 136, 146, 260, 352, 449, 1124
MfeI CAATTG 1 cut(s) 465
MhlI GDGCHC 3 cut(s) 111, 356, 783
MluCI AATT 3 cut(s) 289, 465, 707
MlyI GAGTC 4 cut(s) 14, 401, 595, 1097
MroNI GCCGGC 1 cut(s) 950
MseI TTAA 3 cut(s) 81, 420, 891
MspA1I CMGCKG 2 cut(s) 364, 675
MspI CCGG 3 cut(s) 257, 776, 951
MspR9I CCNGG 1 cut(s) 776
MunI CAATTG 1 cut(s) 465
Mva1269I GAATGC 3 cut(s) 234, 509, 967
MvnI CGCG 1 cut(s) 405
MwoI GCNNNNNNNGC 8 cut(s) 71, 238, 506, 669, 672, 733, 739, 1092
NaeI GCCGGC 1 cut(s) 952
NciI CCSGG 1 cut(s) 776
NdeII GATC 3 cut(s) 442, 982, 1070
NgoMIV GCCGGC 1 cut(s) 950
NlaIII CATG 3 cut(s) 187, 552, 976
NlaIV GGNNCC 3 cut(s) 582, 773, 1038
NmuCI GTSAC 1 cut(s) 451
NspI RCATGY 1 cut(s) 187
PaeI GCATGC 1 cut(s) 187
PaqCI CACCTGC 1 cut(s) 853
PciSI GCTCTTC 1 cut(s) 243
PcsI WCGNNNNNNNCGW 1 cut(s) 117
PctI GAATGC 3 cut(s) 234, 509, 967
PdiI GCCGGC 1 cut(s) 952
PleI GAGTC 4 cut(s) 14, 401, 595, 1096
PpsI GAGTC 4 cut(s) 14, 401, 595, 1096
Ppu21I YACGTR 1 cut(s) 121
PpuMI RGGWCCY 1 cut(s) 1037
PshAI GACNNNNGTC 1 cut(s) 24
Psp124BI GAGCTC 3 cut(s) 111, 356, 783
Psp1406I AACGTT 1 cut(s) 384
Psp5II RGGWCCY 1 cut(s) 1037
PspFI CCCAGC 2 cut(s) 66, 723
PspN4I GGNNCC 3 cut(s) 582, 773, 1038
PspPI GGNCC 3 cut(s) 651, 771, 1037
PspPPI RGGWCCY 1 cut(s) 1037
PvuII CAGCTG 1 cut(s) 364
RsaI GTAC 2 cut(s) 119, 1017
RsaNI GTAC 2 cut(s) 118, 1016
SacI GAGCTC 3 cut(s) 111, 356, 783
SapI GCTCTTC 1 cut(s) 243
SaqAI TTAA 3 cut(s) 81, 420, 891
Sau3AI GATC 3 cut(s) 442, 982, 1070
Sau96I GGNCC 3 cut(s) 651, 771, 1037
SchI GAGTC 4 cut(s) 14, 401, 595, 1097
ScrFI CCNGG 1 cut(s) 776
SduI GDGCHC 3 cut(s) 111, 356, 783
SfaNI GCATC 3 cut(s) 190, 387, 790
SinI GGWCC 3 cut(s) 651, 771, 1037
SmlI CTYRAG 1 cut(s) 748
SmoI CTYRAG 1 cut(s) 748
SphI GCATGC 1 cut(s) 187
Sse9I AATT 3 cut(s) 289, 465, 707
SspI AATATT 1 cut(s) 331
SspMI CTAG 2 cut(s) 648, 1080
SstI GAGCTC 3 cut(s) 111, 356, 783
StyD4I CCNGG 1 cut(s) 774
StyI CCWWGG 3 cut(s) 758, 955, 978
TaaI ACNGT 3 cut(s) 279, 989, 1020
TaiI ACGT 3 cut(s) 123, 387, 1017
TaqI TCGA 3 cut(s) 111, 390, 1069
TaqII GACCGA 1 cut(s) 435
TasI AATT 3 cut(s) 289, 465, 707
TauI GCSGC 3 cut(s) 77, 256, 792
Tru1I TTAA 3 cut(s) 81, 420, 891
Tru9I TTAA 3 cut(s) 81, 420, 891
TscAI CASTG 2 cut(s) 627, 994
TseFI GTSAC 1 cut(s) 451
TseI GCWGC 8 cut(s) 663, 672, 733, 736, 739, 811, 944, 947
Tsp45I GTSAC 1 cut(s) 451
TspDTI ATGAA 3 cut(s) 153, 262, 900
TspGWI ACGGA 1 cut(s) 318
TspRI CASTG 2 cut(s) 627, 994
VpaK11BI GGWCC 3 cut(s) 651, 771, 1037
XceI RCATGY 1 cut(s) 187
XcmI CCANNNNNNNNNTGG 1 cut(s) 765
XspI CTAG 2 cut(s) 648, 1080
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.