MD15G1279200.v1.1
MYB Family

Myb-like DNA-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0006004
Physical Location & Seq
Forward (+)
24728706 .. 24730831
2126 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1279200.v1.1.491

Sequence Viewer

Length: 1149 bp
ATGGAGCTGCTGAGCTTGGACTCCAGTCCTCTGTTCGTCCCGAAAACAATCACCGACTTTCTCGCTCAGCTCTCCGCCATTAAAGACGGCTCTCAGAGGTCTTCAGAGCTCGATGGGAACGTGAAGAGACTGGAAGACGAACTCAGAAAGATTGAAGTTTTCAAGCGGGAGCTTCCTCTCTGCATGCTTCTCCTGAGGGATGTGATTGAGCGGTTGAAGGAGGAAGGAATGCGGTGGAGGCGAATTGAAGAGCGGCCGGTGATGGCGGAGTTCATACCGTGGAAGGGCAATTCTGAGGAGGACGGAGGGGCAGTTTCGGGAAAGGAAAATATTGACAAGAAGAACTGGATGAGCTCTGCGCAGCTATGGAGCACAAGCATCAATGTTTTTGATTACACCAAACACGACTCTGAACTTAAACCGGGGAATGAGGAAGATGATCGGTCGGCGGCGGAGAACCCAATTGAGCTGAGTAATAAGAAAGGAGTGGGAGGGGCATTACTGGCATTCAAGGGTCAGAATGAGTTGTCTGGTTTTGCAAACCCACGTTTGAAGGGAGACAAGGAGGTTACCGTGGTTCCAAACCTTTGTCTTATGACTCCATTGATGTCTGAAGCACTGGCTGGTTCTGCAAATGCAAACTCTAAGACCAACCATGGCTGCAGAGGTGGCGGTTCGGGTTCTGGTATGGCAGGACAATTGAAATTACAGAACAAACCCCAACAGCAGCCACAGCAGCAGCAGCAGCAACCCTTAAGGAAACAAAGGCGGTGCTGGTCGGCGGAGCTCCATCGCCGCTTTGTTGATGCCCTTCAGCAGCTTGGAGGAACACAAGTAGCCACTCCAAAGCAGATAAGAGAACTTATGCAGGTGGAGGGTCTCACCAATGATGAAGTTAAAAGCCACTTGCAGAAATACCGCCTTCACATCCGAAAGCTCCCGCCTTCTTCGGCTGCTGGCCAAGGCAATGACTTACTCGTGCCCTTGGATCACAGTGGAGAGCATACTAAGGCAAACAACGCACAGTCTGGTTCTCCACAGGGTCCTCTCCTCGCAGGAGGGTTTGCCAAAGGCCGATCCACAACTGGAGGCGAGTCGGGCGACAGCAGGGAAGGTGAAGAGGATGAGAAATCGGATGGACAGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Myb_DNA-binding

Protein Analysis

383

Amino Acids

41.97

Weight (kDa)

6.44

Isoelectric Point (pI)

59.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HHO5_N PF26575 5 - 76 3e-29 HHO5-like, N-terminal domain
Myb_DNA-binding PF00249 256 - 307 3.1e-07 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 859
Acc16I TGCGCA 1 cut(s) 360
Acc36I ACCTGC 1 cut(s) 859
AccBSI CCGCTC 2 cut(s) 211, 253
AclWI GGATC 2 cut(s) 996, 1071
AcoI YGGCCR 2 cut(s) 254, 958
AcuI CTGAAG 3 cut(s) 87, 633, 797
AfiI CCNNNNNNNGG 1 cut(s) 284
AflII CTTAAG 1 cut(s) 754
AgsI TTSAA 7 cut(s) 155, 163, 217, 248, 511, 553, 703
Alw21I GWGCWC 4 cut(s) 111, 356, 374, 789
Alw26I GTCTC 3 cut(s) 121, 552, 884
AlwI GGATC 2 cut(s) 996, 1071
AoxI GGCC 3 cut(s) 254, 958, 1072
AspLEI GCGC 1 cut(s) 361
AspS9I GGNCC 1 cut(s) 1043
AsuC2I CCSGG 1 cut(s) 423
AsuHPI GGTGA 4 cut(s) 43, 271, 874, 1127
AvaII GGWCC 1 cut(s) 1043
AxyI CCTNAGG 1 cut(s) 194
BaeGI GKGCMC 1 cut(s) 984
BalI TGGCCA 1 cut(s) 960
BanII GRGCYC 3 cut(s) 111, 356, 789
BauI CACGAG 1 cut(s) 977
BbsI GAAGAC 2 cut(s) 93, 141
Bbv12I GWGCWC 4 cut(s) 111, 356, 374, 789
BbvI GCAGC 9 cut(s) 373, 647, 739, 748, 751, 754, 757, 829, 940
BccI CCATC 4 cut(s) 107, 256, 798, 1130
BceAI ACGGC 1 cut(s) 103
BcnI CCSGG 1 cut(s) 423
BcoDI GTCTC 3 cut(s) 121, 552, 884
BfmI CTRYAG 1 cut(s) 661
BfrI CTTAAG 1 cut(s) 754
BfuAI ACCTGC 1 cut(s) 859
BlpI GCTNAGC 2 cut(s) 11, 66
Bme1390I CCNGG 1 cut(s) 423
Bme18I GGWCC 1 cut(s) 1043
BmgT120I GGNCC 1 cut(s) 1043
BmiI GGNNCC 2 cut(s) 579, 1044
BmrFI CCNGG 1 cut(s) 423
BmsI GCATC 2 cut(s) 387, 796
BoxI GACNNNNGTC 1 cut(s) 24
BpiI GAAGAC 2 cut(s) 93, 141
BpmI CTGGAG 2 cut(s) 7, 1107
Bpu1102I GCTNAGC 2 cut(s) 11, 66
BpuMI CCSGG 1 cut(s) 423
BsaI GGTCTC 1 cut(s) 884
BsaJI CCNNGG 6 cut(s) 278, 422, 573, 655, 961, 984
BsaXI ACNNNNNCTCC 2 cut(s) 297, 327
Bsc4I CCNNNNNNNGG 1 cut(s) 284
Bse118I RCCGGY 1 cut(s) 256
Bse1I ACTGG 6 cut(s) 24, 135, 350, 507, 624, 1090
Bse21I CCTNAGG 1 cut(s) 194
Bse3DI GCAATG 1 cut(s) 973
BseDI CCNNGG 6 cut(s) 278, 422, 573, 655, 961, 984
BseGI GGATG 5 cut(s) 205, 354, 927, 1129, 1141
BseLI CCNNNNNNNGG 1 cut(s) 284
BseMI GCAATG 1 cut(s) 973
BseMII CTCAG 6 cut(s) 80, 107, 157, 185, 285, 461
BseNI ACTGG 6 cut(s) 24, 135, 350, 507, 624, 1090
BseRI GAGGAG 2 cut(s) 311, 1040
BseSI GKGCMC 1 cut(s) 984
BseX3I CGGCCG 1 cut(s) 254
BseXI GCAGC 9 cut(s) 373, 647, 739, 748, 751, 754, 757, 829, 940
Bsh1285I CGRYCG 2 cut(s) 257, 446
BshFI GGCC 3 cut(s) 256, 960, 1074
BsiEI CGRYCG 2 cut(s) 257, 446
BsiHKAI GWGCWC 4 cut(s) 111, 356, 374, 789
BsiSI CCGG 2 cut(s) 257, 422
BslFI GGGAC 1 cut(s) 23
BslI CCNNNNNNNGG 1 cut(s) 284
BsmAI GTCTC 3 cut(s) 121, 552, 884
BsmFI GGGAC 1 cut(s) 23
BsmI GAATGC 2 cut(s) 234, 506
BsnI GGCC 3 cut(s) 256, 960, 1074
Bso31I GGTCTC 1 cut(s) 884
Bsp1286I GDGCHC 5 cut(s) 111, 356, 374, 789, 984
Bsp143I GATC 3 cut(s) 439, 988, 1076
Bsp1720I GCTNAGC 2 cut(s) 11, 66
Bsp19I CCATGG 1 cut(s) 655
BspANI GGCC 3 cut(s) 256, 960, 1074
BspCNI CTCAG 6 cut(s) 79, 106, 156, 186, 286, 462
BspLI GGNNCC 2 cut(s) 579, 1044
BspMAI CTGCAG 1 cut(s) 665
BspMI ACCTGC 1 cut(s) 859
BspPI GGATC 2 cut(s) 996, 1071
BspQI GCTCTTC 1 cut(s) 243
BspTI CTTAAG 1 cut(s) 754
BspTNI GGTCTC 1 cut(s) 884
BsrBI CCGCTC 2 cut(s) 211, 253
BsrDI GCAATG 1 cut(s) 973
BsrFI RCCGGY 1 cut(s) 256
BsrI ACTGG 6 cut(s) 24, 135, 350, 507, 624, 1090
BssAI RCCGGY 1 cut(s) 256
BssECI CCNNGG 6 cut(s) 278, 422, 573, 655, 961, 984
BssMI GATC 3 cut(s) 439, 988, 1076
BssSI CACGAG 1 cut(s) 977
BssT1I CCWWGG 3 cut(s) 655, 961, 984
Bst2BI CACGAG 1 cut(s) 977
Bst4CI ACNGT 4 cut(s) 279, 574, 995, 1026
Bst6I CTCTTC 3 cut(s) 119, 243, 1113
BstAFI CTTAAG 1 cut(s) 754
BstC8I GCNNGC 2 cut(s) 185, 958
BstDEI CTNAG 9 cut(s) 11, 66, 93, 143, 194, 294, 470, 645, 1008
BstDSI CCRYGG 3 cut(s) 278, 573, 655
BstEII GGTNACC 1 cut(s) 568
BstF5I GGATG 5 cut(s) 205, 354, 927, 1129, 1141
BstHHI GCGC 1 cut(s) 361
BstKTI GATC 3 cut(s) 442, 991, 1079
BstMAI GTCTC 3 cut(s) 121, 552, 884
BstMBI GATC 3 cut(s) 439, 988, 1076
BstMCI CGRYCG 2 cut(s) 257, 446
BstNSI RCATGY 1 cut(s) 187
BstPAI GACNNNNGTC 1 cut(s) 24
BstPI GGTNACC 1 cut(s) 568
BstSCI CCNGG 1 cut(s) 421
BstSFI CTRYAG 1 cut(s) 661
BstSLI GKGCMC 1 cut(s) 984
BstV1I GCAGC 9 cut(s) 373, 647, 739, 748, 751, 754, 757, 829, 940
BstV2I GAAGAC 2 cut(s) 93, 141
BstZI CGGCCG 1 cut(s) 254
Bsu36I CCTNAGG 1 cut(s) 194
BsuRI GGCC 3 cut(s) 256, 960, 1074
BtgI CCRYGG 3 cut(s) 278, 573, 655
BtgZI GCGATG 1 cut(s) 776
BtsCI GGATG 5 cut(s) 205, 354, 927, 1129, 1141
BtsIMutI CAGTG 2 cut(s) 617, 1000
BveI ACCTGC 1 cut(s) 859
Cac8I GCNNGC 2 cut(s) 185, 958
CfoI GCGC 1 cut(s) 361
Cfr10I RCCGGY 1 cut(s) 256
Cfr13I GGNCC 1 cut(s) 1043
CviAII CATG 2 cut(s) 184, 656
DdeI CTNAG 9 cut(s) 11, 66, 93, 143, 194, 294, 470, 645, 1008
DpnI GATC 3 cut(s) 441, 990, 1078
DpnII GATC 3 cut(s) 439, 988, 1076
EaeI YGGCCR 2 cut(s) 254, 958
EagI CGGCCG 1 cut(s) 254
Eam1104I CTCTTC 3 cut(s) 119, 243, 1113
EarI CTCTTC 3 cut(s) 119, 243, 1113
EciI GGCGGA 4 cut(s) 64, 281, 467, 797
Ecl136II GAGCTC 3 cut(s) 109, 354, 787
EclXI CGGCCG 1 cut(s) 254
Eco130I CCWWGG 3 cut(s) 655, 961, 984
Eco24I GRGCYC 3 cut(s) 111, 356, 789
Eco31I GGTCTC 1 cut(s) 884
Eco47I GGWCC 1 cut(s) 1043
Eco52I CGGCCG 1 cut(s) 254
Eco53kI GAGCTC 3 cut(s) 109, 354, 787
Eco57I CTGAAG 3 cut(s) 87, 633, 797
Eco81I CCTNAGG 1 cut(s) 194
Eco91I GGTNACC 1 cut(s) 568
EcoICRI GAGCTC 3 cut(s) 109, 354, 787
EcoO109I RGGNCCY 1 cut(s) 1043
EcoO65I GGTNACC 1 cut(s) 568
EcoT14I CCWWGG 3 cut(s) 655, 961, 984
EcoT38I GRGCYC 3 cut(s) 111, 356, 789
ErhI CCWWGG 3 cut(s) 655, 961, 984
FaeI CATG 2 cut(s) 187, 659
FaiI YATR 8 cut(s) 185, 275, 367, 596, 657, 689, 866, 1005
FaqI GGGAC 1 cut(s) 23
FatI CATG 2 cut(s) 183, 655
FauI CCCGC 2 cut(s) 159, 948
FokI GGATG 4 cut(s) 212, 361, 914, 1136
FriOI GRGCYC 3 cut(s) 111, 356, 789
FspI TGCGCA 1 cut(s) 360
GlaI GCGC 1 cut(s) 360
GsuI CTGGAG 2 cut(s) 7, 1107
HaeIII GGCC 3 cut(s) 256, 960, 1074
HapII CCGG 2 cut(s) 257, 422
HhaI GCGC 1 cut(s) 361
Hin1II CATG 2 cut(s) 187, 659
Hin6I GCGC 1 cut(s) 359
HinP1I GCGC 1 cut(s) 359
HinfI GANTC 4 cut(s) 20, 407, 598, 1094
HpaII CCGG 2 cut(s) 257, 422
HphI GGTGA 4 cut(s) 43, 271, 874, 1127
Hpy188I TCNGA 9 cut(s) 96, 106, 146, 295, 412, 519, 613, 932, 1135
Hpy188III TCNNGA 3 cut(s) 40, 193, 318
HpyAV CCTTC 8 cut(s) 211, 218, 277, 547, 821, 932, 954, 1106
HpyCH4III ACNGT 4 cut(s) 279, 574, 995, 1026
HpyCH4IV ACGT 2 cut(s) 120, 547
HpyCH4V TGCA 7 cut(s) 183, 539, 632, 638, 663, 868, 910
HpyF3I CTNAG 9 cut(s) 11, 66, 93, 143, 194, 294, 470, 645, 1008
HpySE526I ACGT 2 cut(s) 120, 547
Hsp92II CATG 2 cut(s) 187, 659
HspAI GCGC 1 cut(s) 359
Kzo9I GATC 3 cut(s) 439, 988, 1076
LguI GCTCTTC 1 cut(s) 243
LmnI GCTCC 6 cut(s) 4, 169, 369, 784, 792, 942
Lsp1109I GCAGC 9 cut(s) 373, 647, 739, 748, 751, 754, 757, 829, 940
LweI GCATC 2 cut(s) 387, 796
MaeII ACGT 2 cut(s) 120, 547
MaeIII GTNAC 1 cut(s) 568
MalI GATC 3 cut(s) 441, 990, 1078
MbiI CCGCTC 2 cut(s) 211, 253
MboI GATC 3 cut(s) 439, 988, 1076
MboII GAAGA 8 cut(s) 93, 136, 146, 260, 352, 446, 939, 1130
MfeI CAATTG 2 cut(s) 462, 698
MhlI GDGCHC 5 cut(s) 111, 356, 374, 789, 984
MlsI TGGCCA 1 cut(s) 960
MluCI AATT 5 cut(s) 243, 289, 462, 698, 704
MluNI TGGCCA 1 cut(s) 960
MlyI GAGTC 4 cut(s) 14, 401, 592, 1103
Mox20I TGGCCA 1 cut(s) 960
MscI TGGCCA 1 cut(s) 960
MseI TTAA 4 cut(s) 81, 417, 755, 897
Msp20I TGGCCA 1 cut(s) 960
MspCI CTTAAG 1 cut(s) 754
MspI CCGG 2 cut(s) 257, 422
MspR9I CCNGG 1 cut(s) 423
MunI CAATTG 2 cut(s) 462, 698
Mva1269I GAATGC 2 cut(s) 234, 506
NciI CCSGG 1 cut(s) 423
NcoI CCATGG 1 cut(s) 655
NdeII GATC 3 cut(s) 439, 988, 1076
NlaIII CATG 2 cut(s) 187, 659
NlaIV GGNNCC 2 cut(s) 579, 1044
NsbI TGCGCA 1 cut(s) 360
NspI RCATGY 1 cut(s) 187
PaeI GCATGC 1 cut(s) 187
PaqCI CACCTGC 1 cut(s) 859
PciSI GCTCTTC 1 cut(s) 243
PcsI WCGNNNNNNNCGW 1 cut(s) 117
PctI GAATGC 2 cut(s) 234, 506
PleI GAGTC 4 cut(s) 14, 401, 592, 1102
PpsI GAGTC 4 cut(s) 14, 401, 592, 1102
PpuMI RGGWCCY 1 cut(s) 1043
PshAI GACNNNNGTC 1 cut(s) 24
Psp124BI GAGCTC 3 cut(s) 111, 356, 789
Psp5II RGGWCCY 1 cut(s) 1043
PspEI GGTNACC 1 cut(s) 568
PspN4I GGNNCC 2 cut(s) 579, 1044
PspPI GGNCC 1 cut(s) 1043
PspPPI RGGWCCY 1 cut(s) 1043
PstI CTGCAG 1 cut(s) 665
SacI GAGCTC 3 cut(s) 111, 356, 789
SapI GCTCTTC 1 cut(s) 243
SaqAI TTAA 4 cut(s) 81, 417, 755, 897
Sau3AI GATC 3 cut(s) 439, 988, 1076
Sau96I GGNCC 1 cut(s) 1043
SchI GAGTC 4 cut(s) 14, 401, 592, 1103
ScrFI CCNGG 1 cut(s) 423
SduI GDGCHC 5 cut(s) 111, 356, 374, 789, 984
SfaNI GCATC 2 cut(s) 387, 796
SfcI CTRYAG 1 cut(s) 661
SinI GGWCC 1 cut(s) 1043
SmlI CTYRAG 1 cut(s) 754
SmoI CTYRAG 1 cut(s) 754
SphI GCATGC 1 cut(s) 187
Sse9I AATT 5 cut(s) 243, 289, 462, 698, 704
SspI AATATT 1 cut(s) 331
SstI GAGCTC 3 cut(s) 111, 356, 789
StyD4I CCNGG 1 cut(s) 421
StyI CCWWGG 3 cut(s) 655, 961, 984
TaaI ACNGT 4 cut(s) 279, 574, 995, 1026
TaiI ACGT 2 cut(s) 123, 550
TaqI TCGA 1 cut(s) 111
TaqII GACCGA 1 cut(s) 432
TasI AATT 5 cut(s) 243, 289, 462, 698, 704
TauI GCSGC 3 cut(s) 256, 452, 798
Tru1I TTAA 4 cut(s) 81, 417, 755, 897
Tru9I TTAA 4 cut(s) 81, 417, 755, 897
TscAI CASTG 2 cut(s) 624, 1000
TspDTI ATGAA 2 cut(s) 262, 906
TspGWI ACGGA 1 cut(s) 318
TspRI CASTG 2 cut(s) 624, 1000
Vha464I CTTAAG 1 cut(s) 754
VpaK11BI GGWCC 1 cut(s) 1043
XceI RCATGY 1 cut(s) 187
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.