Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0001650

Physical Location & Seq

Genomic Coordinates Forward (+)

17165594 .. 17168870

Gene Structure 3277 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1187800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1239 bp

ATGGGGAGCGAAATCGAAGAATTTGAAGATCCAAAACTTTCATCCGAAGCATTGGAGAACAATCGTGCATCTTCGTCGAAGGAAGGCAAAGGAAAGTGGCTGTGGGAGAAGGTGAAGTATCAGCTGGTTGAGTACCACACTTTGCCAAATTACTTGAGGGACAATGAGTTCATCTTAGGCCATTACCGGTCTGAATGGCCATTGAAGCAGGTCTTGCTTAGTATGTTTACCATCCACAACGAGACGCTGAACGTTTGGACGCACTTGATCGGATTCTTCCTTTTTCTTTCCTTGACCATATACACTGCAAATAAGGTTCCAGAGGTCGTTGATCTTCATGCTCTGCAGCATTTTCCTGATGTGCTGAAAAAGGCGGATCTGCACAAATTACATGAAGAATTCATGACCTGCCTCCCTTCCTTACCCAACATGCCAGATCTTAACAGACTTAGAGAAGAGTTAAAGATGTCGTTAACTTCAATCGATCTGCTACCCTCACTCTCTGGTTGGCATGTTATGGAACTTCTGAAAAACTGTTTGCCACAACGATTCTCCCATGGCAACCAAACTGATGTCTGTGTGCTGCGTAGCATGAAGGAGGATGTAGCAAACATAATAGCACCATTGATGATTAGACCAATCACAAGATGGCCATTTTTTGCCTTCTTGGGGGGAGCCATGTTTTGTTTGCTGACCAGCAGCGCATGCCATCTCCTCTCTTGCCACTCTGAGCTCATGTCGTATATCATGCTCAGGATGGACTATGCTGGAATTGCAGCCCTTATATCTACCTCATTCTACCCTCTCGTCTACTACAGTTTCATGTGTAACCCTTTCTTGTGCGCCCTCTACATGGGATGCATAACCCTCTTGGGAATTGCCACAGTTTTGGTTTCCCTTATCCCAGTCTTTCAAAATCCTGAATACCGAATAGTCCGTGTGTCTCTTTTTCTTGGCATGGGCTTGTCCGGGATTCTACCTGTTATGCACAAGCTGATCTTGTTTTCAGATCAACCTGAGGCACTCCACACAACCGGGTATGAGATTCTGATGGGCCTTCTCTACGGGGTGGGAGCCCTGGTCTATGCCACAAGGATTCCGGAACGATGGCTGCCTGGGAAATTCGACATAGCAGGGCACAGCCACCAGCTGTTTCACGTATTGGTAGTGGCGGGGGCATACACCCATTACCGTGCAGGGCTGGTGTACCTCCAGTGGCGCGACTTAGCGGGTTGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0008150 GO:0009719 GO:0009725 GO:0009743 GO:0009744 GO:0010033 GO:0034285 GO:0042221 GO:0050896 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko04152 ko04211 ko04920 ko04932 map04152 map04211 map04920 map04932

Pfam Domains

Protein Families

HlyIII

Protein Analysis

413

Amino Acids

46.99

Weight (kDa)

6.41

Isoelectric Point (pI)

51.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HlyIII	PF03006	76 - 106	4.9e-06	Haemolysin-III related
HlyIII	PF03006	212 - 397	8.4e-55	Haemolysin-III related

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0001650)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G37680 AT4G37680 AT4G37680 AT4G37680 AT4G37680 AT4G38320
fragaria_vesca	FvH4_1g17920 FvH4_1g17920 FvH4_1g17920 FvH4_5g20320 FvH4_5g20320 FvH4_5g20320
malus_domestica	MD02G1187800.v1.1 MD04G1053000.v1.1 MD06G1047400.v1.1 MD15G1298700.v1.1
prunus_persica	Prupe.5G057500_v2.0.a1 Prupe.5G057500_v2.0.a1 Prupe.5G057500_v2.0.a1 Prupe.5G057500_v2.0.a1 Prupe.6G219200_v2.0.a1 Prupe.6G219200_v2.0.a1
pyrus_communis	pycom02g15270 pycom04g04650 pycom06g04030 pycom15g26150
rosa_chinensis	RchiOBHm_Chr2g0108351 RchiOBHm_Chr7g0205491
rosa_laevigata	RLG00000003374 RLG00000017663
rosa_multiflora	Rmu_sc0002237.1_g000016 Rmu_sc0002372.1_g000016 Rmu_sc0041421.1_g000001
rosa_roxburghii	Rroxscaffold_2G00135220 Rroxscaffold_3G00252180
rosa_rugosa	Rorug02G0152200 Rorug07G0091600 Rorug07G0091700
rosa_samantha	Rh2AG201200 Rh2BG214100 Rh2CG205500 Rh2DG208600 Rh7AG222300 Rh7BG217700 Rh7CG234700 Rh7DG228700
rosa_wichuraiana	Rw2G016020 Rw2G016080 Rw7G019190

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	199, 416
AccI	GTMKAC	1 cut(s)	810
AccII	CGCG	1 cut(s)	1221
AccIII	TCCGGA	1 cut(s)	1099
AciI	CCGC	3 cut(s)	374, 1172, 1229
AclI	AACGTT	1 cut(s)	252
AclWI	GGATC	2 cut(s)	23, 384
AcoI	YGGCCR	2 cut(s)	197, 650
AcsI	RAATTY	3 cut(s)	20, 398, 1121
AfaI	GTAC	2 cut(s)	134, 1208
AfiI	CCNNNNNNNGG	2 cut(s)	669, 853
AgeI	ACCGGT	1 cut(s)	186
AgsI	TTSAA	4 cut(s)	26, 205, 480, 914
AjnI	CCWGG	2 cut(s)	1077, 1114
AloI	GAACNNNNNNTCC	2 cut(s)	152, 184
AluBI	AGCT	4 cut(s)	124, 733, 994, 1150
AluI	AGCT	4 cut(s)	124, 733, 994, 1150
Alw21I	GWGCWC	1 cut(s)	735
Alw26I	GTCTC	2 cut(s)	236, 948
AlwI	GGATC	2 cut(s)	23, 384
Aor13HI	TCCGGA	1 cut(s)	1099
AoxI	GGCC	4 cut(s)	178, 197, 650, 1054
ApeKI	GCWGC	5 cut(s)	346, 583, 699, 776, 1111
ApoI	RAATTY	3 cut(s)	20, 398, 1121
AsiGI	ACCGGT	1 cut(s)	186
AspLEI	GCGC	3 cut(s)	704, 845, 1221
AspS9I	GGNCC	1 cut(s)	1054
AsuC2I	CCSGG	2 cut(s)	970, 1036
AsuHPI	GGTGA	1 cut(s)	124
AxyI	CCTNAGG	1 cut(s)	1017
BaeGI	GKGCMC	1 cut(s)	1140
BalI	TGGCCA	2 cut(s)	199, 652
BanII	GRGCYC	2 cut(s)	735, 1078
Bbv12I	GWGCWC	1 cut(s)	735
BbvI	GCAGC	5 cut(s)	358, 570, 711, 788, 1098
BccI	CCATC	6 cut(s)	239, 642, 717, 751, 1045, 1101
BcgI	CGANNNNNNTGC	2 cut(s)	57, 91
BciT130I	CCWGG	2 cut(s)	1079, 1116
BcnI	CCSGG	2 cut(s)	970, 1036
BcoDI	GTCTC	2 cut(s)	236, 948
BfmI	CTRYAG	2 cut(s)	344, 814
BfuAI	ACCTGC	2 cut(s)	199, 416
BglII	AGATCT	1 cut(s)	436
BisI	GCNGC	5 cut(s)	347, 584, 700, 777, 1112
BlsI	GCNGC	5 cut(s)	348, 585, 701, 778, 1113
Bme1390I	CCNGG	4 cut(s)	970, 1036, 1079, 1116
BmgT120I	GGNCC	1 cut(s)	1054
BmiI	GGNNCC	3 cut(s)	318, 676, 1075
BmrFI	CCNGG	4 cut(s)	970, 1036, 1079, 1116
BmrI	ACTGGG	1 cut(s)	899
BmsI	GCATC	2 cut(s)	77, 848
BmuI	ACTGGG	1 cut(s)	899
BpmI	CTGGAG	1 cut(s)	1196
Bpu10I	CCTNAGC	1 cut(s)	752
BpuEI	CTTGAG	1 cut(s)	175
BpuMI	CCSGG	2 cut(s)	970, 1036
Bsa29I	ATCGAT	1 cut(s)	483
BsaAI	YACGTR	1 cut(s)	1159
BsaJI	CCNNGG	3 cut(s)	556, 1077, 1115
BsaWI	WCCGGW	2 cut(s)	186, 1099
BsaXI	ACNNNNNCTCC	2 cut(s)	1065, 1095
Bsc4I	CCNNNNNNNGG	2 cut(s)	669, 853
Bse118I	RCCGGY	1 cut(s)	186
Bse1I	ACTGG	2 cut(s)	905, 1213
Bse21I	CCTNAGG	1 cut(s)	1017
BseAI	TCCGGA	1 cut(s)	1099
BseBI	CCWGG	2 cut(s)	1079, 1116
BseCI	ATCGAT	1 cut(s)	483
BseDI	CCNNGG	3 cut(s)	556, 1077, 1115
BseGI	GGATG	5 cut(s)	41, 231, 607, 762, 863
BseLI	CCNNNNNNNGG	2 cut(s)	669, 853
BseMII	CTCAG	3 cut(s)	720, 766, 1008
BseNI	ACTGG	2 cut(s)	905, 1213
BseRI	GAGGAG	1 cut(s)	704
BseSI	GKGCMC	1 cut(s)	1140
BseXI	GCAGC	5 cut(s)	358, 570, 711, 788, 1098
BsgI	GTGCAG	2 cut(s)	365, 1215
Bsh1236I	CGCG	1 cut(s)	1221
BshFI	GGCC	4 cut(s)	180, 199, 652, 1056
BshTI	ACCGGT	1 cut(s)	186
BshVI	ATCGAT	1 cut(s)	483
BsiHKAI	GWGCWC	1 cut(s)	735
BsiSI	CCGG	4 cut(s)	187, 969, 1035, 1100
BslFI	GGGAC	1 cut(s)	173
BslI	CCNNNNNNNGG	2 cut(s)	669, 853
BsmAI	GTCTC	2 cut(s)	236, 948
BsmBI	CGTCTC	1 cut(s)	236
BsmFI	GGGAC	1 cut(s)	173
BsnI	GGCC	4 cut(s)	180, 199, 652, 1056
Bsp1286I	GDGCHC	3 cut(s)	735, 1078, 1140
Bsp13I	TCCGGA	1 cut(s)	1099
Bsp143I	GATC	8 cut(s)	28, 267, 331, 376, 436, 484, 996, 1009
Bsp19I	CCATGG	1 cut(s)	556
BspACI	CCGC	3 cut(s)	374, 1172, 1229
BspANI	GGCC	4 cut(s)	180, 199, 652, 1056
BspCNI	CTCAG	3 cut(s)	721, 765, 1009
BspDI	ATCGAT	1 cut(s)	483
BspEI	TCCGGA	1 cut(s)	1099
BspFNI	CGCG	1 cut(s)	1221
BspHI	TCATGA	1 cut(s)	402
BspLI	GGNNCC	3 cut(s)	318, 676, 1075
BspMAI	CTGCAG	1 cut(s)	348
BspMI	ACCTGC	2 cut(s)	199, 416
BspPI	GGATC	2 cut(s)	23, 384
BsrFI	RCCGGY	1 cut(s)	186
BsrI	ACTGG	2 cut(s)	905, 1213
BssAI	RCCGGY	1 cut(s)	186
BssECI	CCNNGG	3 cut(s)	556, 1077, 1115
BssMI	GATC	8 cut(s)	28, 267, 331, 376, 436, 484, 996, 1009
BssT1I	CCWWGG	1 cut(s)	556
Bst2UI	CCWGG	2 cut(s)	1079, 1116
Bst4CI	ACNGT	4 cut(s)	536, 818, 886, 1193
Bst6I	CTCTTC	1 cut(s)	450
BstAPI	GCANNNNNTGC	2 cut(s)	214, 705
BstBAI	YACGTR	1 cut(s)	1159
BstC8I	GCNNGC	1 cut(s)	706
BstDEI	CTNAG	7 cut(s)	175, 218, 449, 729, 752, 1017, 1225
BstDSI	CCRYGG	1 cut(s)	556
BstF5I	GGATG	5 cut(s)	41, 231, 607, 762, 863
BstFNI	CGCG	1 cut(s)	1221
BstHHI	GCGC	3 cut(s)	704, 845, 1221
BstKTI	GATC	8 cut(s)	31, 270, 334, 379, 439, 487, 999, 1012
BstMAI	GTCTC	2 cut(s)	236, 948
BstMBI	GATC	8 cut(s)	28, 267, 331, 376, 436, 484, 996, 1009
BstMWI	GCNNNNNNNGC	4 cut(s)	205, 214, 705, 773
BstNI	CCWGG	2 cut(s)	1079, 1116
BstNSI	RCATGY	3 cut(s)	433, 515, 708
BstSCI	CCNGG	4 cut(s)	968, 1034, 1077, 1114
BstSFI	CTRYAG	2 cut(s)	344, 814
BstSLI	GKGCMC	1 cut(s)	1140
BstUI	CGCG	1 cut(s)	1221
BstV1I	GCAGC	5 cut(s)	358, 570, 711, 788, 1098
BstX2I	RGATCY	3 cut(s)	28, 376, 436
BstXI	CCANNNNNNTGG	1 cut(s)	889
BstYI	RGATCY	3 cut(s)	28, 376, 436
Bsu15I	ATCGAT	1 cut(s)	483
Bsu36I	CCTNAGG	1 cut(s)	1017
BsuRI	GGCC	4 cut(s)	180, 199, 652, 1056
BsuTUI	ATCGAT	1 cut(s)	483
BtgI	CCRYGG	1 cut(s)	556
BtsCI	GGATG	5 cut(s)	41, 231, 607, 762, 863
BtsI	GCAGTG	1 cut(s)	303
BtsIMutI	CAGTG	2 cut(s)	303, 1220
BveI	ACCTGC	2 cut(s)	199, 416
Cac8I	GCNNGC	1 cut(s)	706
CciI	TCATGA	1 cut(s)	402
CfoI	GCGC	3 cut(s)	704, 845, 1221
Cfr10I	RCCGGY	1 cut(s)	186
Cfr13I	GGNCC	1 cut(s)	1054
ClaI	ATCGAT	1 cut(s)	483
CseI	GACGC	2 cut(s)	253, 268
Csp6I	GTAC	2 cut(s)	133, 1207
CspAI	ACCGGT	1 cut(s)	186
CviQI	GTAC	2 cut(s)	133, 1207
DdeI	CTNAG	7 cut(s)	175, 218, 449, 729, 752, 1017, 1225
DpnI	GATC	8 cut(s)	30, 269, 333, 378, 438, 486, 998, 1011
DpnII	GATC	8 cut(s)	28, 267, 331, 376, 436, 484, 996, 1009
EaeI	YGGCCR	2 cut(s)	197, 650
Eam1104I	CTCTTC	1 cut(s)	450
EarI	CTCTTC	1 cut(s)	450
EciI	GGCGGA	1 cut(s)	389
Ecl136II	GAGCTC	1 cut(s)	733
Eco130I	CCWWGG	1 cut(s)	556
Eco24I	GRGCYC	2 cut(s)	735, 1078
Eco53kI	GAGCTC	1 cut(s)	733
Eco81I	CCTNAGG	1 cut(s)	1017
EcoICRI	GAGCTC	1 cut(s)	733
EcoRI	GAATTC	1 cut(s)	398
EcoRII	CCWGG	2 cut(s)	1077, 1114
EcoT14I	CCWWGG	1 cut(s)	556
EcoT22I	ATGCAT	1 cut(s)	863
EcoT38I	GRGCYC	2 cut(s)	735, 1078
ErhI	CCWWGG	1 cut(s)	556
Esp3I	CGTCTC	1 cut(s)	236
FalI	AAGNNNNNCTT	4 cut(s)	197, 229, 983, 1015
FaqI	GGGAC	1 cut(s)	173
FauI	CCCGC	2 cut(s)	1165, 1222
FblI	GTMKAC	1 cut(s)	810
Fnu4HI	GCNGC	5 cut(s)	347, 584, 700, 777, 1112
FokI	GGATG	5 cut(s)	28, 218, 614, 769, 870
FriOI	GRGCYC	2 cut(s)	735, 1078
Fsp4HI	GCNGC	5 cut(s)	347, 584, 700, 777, 1112
GlaI	GCGC	3 cut(s)	703, 844, 1220
GluI	GCNGC	5 cut(s)	347, 584, 700, 777, 1112
GsuI	CTGGAG	1 cut(s)	1196
HaeIII	GGCC	4 cut(s)	180, 199, 652, 1056
HapII	CCGG	4 cut(s)	187, 969, 1035, 1100
HgaI	GACGC	2 cut(s)	253, 268
HhaI	GCGC	3 cut(s)	704, 845, 1221
Hin6I	GCGC	3 cut(s)	702, 843, 1219
HinP1I	GCGC	3 cut(s)	702, 843, 1219
HincII	GTYRAC	1 cut(s)	474
HindII	GTYRAC	1 cut(s)	474
HinfI	GANTC	5 cut(s)	273, 549, 973, 1045, 1096
HpaI	GTTAAC	1 cut(s)	474
HpaII	CCGG	4 cut(s)	187, 969, 1035, 1100
HphI	GGTGA	1 cut(s)	124
Hpy166II	GTNNAC	4 cut(s)	228, 474, 811, 1207
Hpy188I	TCNGA	7 cut(s)	46, 193, 272, 528, 730, 1009, 1050
Hpy188III	TCNNGA	6 cut(s)	320, 356, 403, 754, 920, 1100
Hpy8I	GTNNAC	4 cut(s)	228, 474, 811, 1207
Hpy99I	CGWCG	1 cut(s)	79
HpyAV	CCTTC	7 cut(s)	73, 77, 103, 426, 589, 673, 1067
HpyCH4III	ACNGT	4 cut(s)	536, 818, 886, 1193
HpyCH4IV	ACGT	2 cut(s)	252, 1158
HpyCH4V	TGCA	8 cut(s)	68, 308, 346, 382, 776, 861, 988, 1196
HpyF10VI	GCNNNNNNNGC	4 cut(s)	205, 214, 705, 773
HpyF3I	CTNAG	7 cut(s)	175, 218, 449, 729, 752, 1017, 1225
HpySE526I	ACGT	2 cut(s)	252, 1158
HspAI	GCGC	3 cut(s)	702, 843, 1219
Kpn2I	TCCGGA	1 cut(s)	1099
KspAI	GTTAAC	1 cut(s)	474
Kzo9I	GATC	8 cut(s)	28, 267, 331, 376, 436, 484, 996, 1009
LmnI	GCTCC	3 cut(s)	6, 674, 1073
Lsp1109I	GCAGC	5 cut(s)	358, 570, 711, 788, 1098
LweI	GCATC	2 cut(s)	77, 848
MaeII	ACGT	2 cut(s)	252, 1158
MaeIII	GTNAC	1 cut(s)	827
MalI	GATC	8 cut(s)	30, 269, 333, 378, 438, 486, 998, 1011
MboI	GATC	8 cut(s)	28, 267, 331, 376, 436, 484, 996, 1009
MboII	GAAGA	7 cut(s)	29, 38, 63, 268, 326, 407, 467
MflI	RGATCY	3 cut(s)	28, 376, 436
MhlI	GDGCHC	3 cut(s)	735, 1078, 1140
MlsI	TGGCCA	2 cut(s)	199, 652
MluCI	AATT	7 cut(s)	20, 148, 386, 398, 771, 876, 1121
MluNI	TGGCCA	2 cut(s)	199, 652
Mox20I	TGGCCA	2 cut(s)	199, 652
Mph1103I	ATGCAT	1 cut(s)	863
MroI	TCCGGA	1 cut(s)	1099
MscI	TGGCCA	2 cut(s)	199, 652
MseI	TTAA	3 cut(s)	441, 461, 473
MslI	CAYNNNNRTG	1 cut(s)	1191
Msp20I	TGGCCA	2 cut(s)	199, 652
MspA1I	CMGCKG	2 cut(s)	124, 1150
MspI	CCGG	4 cut(s)	187, 969, 1035, 1100
MspR9I	CCNGG	4 cut(s)	970, 1036, 1079, 1116
MvaI	CCWGG	2 cut(s)	1079, 1116
MvnI	CGCG	1 cut(s)	1221
MwoI	GCNNNNNNNGC	4 cut(s)	205, 214, 705, 773
NciI	CCSGG	2 cut(s)	970, 1036
NcoI	CCATGG	1 cut(s)	556
NdeII	GATC	8 cut(s)	28, 267, 331, 376, 436, 484, 996, 1009
NlaIV	GGNNCC	3 cut(s)	318, 676, 1075
NsiI	ATGCAT	1 cut(s)	863
NspI	RCATGY	3 cut(s)	433, 515, 708
PaeI	GCATGC	1 cut(s)	708
PagI	TCATGA	1 cut(s)	402
PfeI	GAWTC	5 cut(s)	273, 549, 973, 1045, 1096
PfoI	TCCNGGA	1 cut(s)	968
PinAI	ACCGGT	1 cut(s)	186
PkrI	GCNGC	5 cut(s)	348, 585, 701, 778, 1113
Ppu21I	YACGTR	1 cut(s)	1159
Psp124BI	GAGCTC	1 cut(s)	735
Psp1406I	AACGTT	1 cut(s)	252
Psp6I	CCWGG	2 cut(s)	1077, 1114
PspGI	CCWGG	2 cut(s)	1077, 1114
PspN4I	GGNNCC	3 cut(s)	318, 676, 1075
PspPI	GGNCC	1 cut(s)	1054
PstI	CTGCAG	1 cut(s)	348
PsuI	RGATCY	3 cut(s)	28, 376, 436
PvuII	CAGCTG	2 cut(s)	124, 1150
RsaI	GTAC	2 cut(s)	134, 1208
RsaNI	GTAC	2 cut(s)	133, 1207
RseI	CAYNNNNRTG	1 cut(s)	1191
SacI	GAGCTC	1 cut(s)	735
SaqAI	TTAA	3 cut(s)	441, 461, 473
SatI	GCNGC	5 cut(s)	347, 584, 700, 777, 1112
Sau3AI	GATC	8 cut(s)	28, 267, 331, 376, 436, 484, 996, 1009
Sau96I	GGNCC	1 cut(s)	1054
ScrFI	CCNGG	4 cut(s)	970, 1036, 1079, 1116
SduI	GDGCHC	3 cut(s)	735, 1078, 1140
SfaNI	GCATC	2 cut(s)	77, 848
SfcI	CTRYAG	2 cut(s)	344, 814
SmiMI	CAYNNNNRTG	1 cut(s)	1191
SmlI	CTYRAG	1 cut(s)	154
SmoI	CTYRAG	1 cut(s)	154
SphI	GCATGC	1 cut(s)	708
Sse9I	AATT	7 cut(s)	20, 148, 386, 398, 771, 876, 1121
SsiI	CCGC	3 cut(s)	374, 1172, 1229
SstI	GAGCTC	1 cut(s)	735
StyD4I	CCNGG	4 cut(s)	968, 1034, 1077, 1114
StyI	CCWWGG	1 cut(s)	556
TaaI	ACNGT	4 cut(s)	536, 818, 886, 1193
TaiI	ACGT	2 cut(s)	255, 1161
TaqI	TCGA	4 cut(s)	15, 77, 483, 1125
TasI	AATT	7 cut(s)	20, 148, 386, 398, 771, 876, 1121
TfiI	GAWTC	5 cut(s)	273, 549, 973, 1045, 1096
Tru1I	TTAA	3 cut(s)	441, 461, 473
Tru9I	TTAA	3 cut(s)	441, 461, 473
TscAI	CASTG	2 cut(s)	310, 1220
TseI	GCWGC	5 cut(s)	346, 583, 699, 776, 1111
TspDTI	ATGAA	7 cut(s)	30, 160, 326, 391, 408, 608, 811
TspGWI	ACGGA	1 cut(s)	926
TspRI	CASTG	2 cut(s)	310, 1220
XapI	RAATTY	3 cut(s)	20, 398, 1121
XceI	RCATGY	3 cut(s)	433, 515, 708
XcmI	CCANNNNNNNNNTGG	1 cut(s)	645
XmiI	GTMKAC	1 cut(s)	810
Zsp2I	ATGCAT	1 cut(s)	863

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.