MD06G1047400.v1.1

Haemolysin-III related

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0001650
Physical Location & Seq
Forward (+)
6439210 .. 6442222
3013 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1047400.v1.1.491

Sequence Viewer

Length: 1194 bp
ATGGGTGGTGATTCTCCATTATCAGAAACAAGATCATTAGATAATCAATTTGACTGTGAAGATGTAAACGAAGCATGTTTAAAGGAAGCAAAGGGAAAGAGACTATGGAAGAAAGTTAGGTATCAGCTTATTGAATACCATTCATTGCCTGGTTTTTTAAGAGACAATGAGTTCATTCTGGGTTATTACCGATCAGAATGGCCATTGAAGCAGGTGTTTCTAAGCATTTTCTCCATTCATAATGAGACTCTTAATGTCTGGACGCATTTGATCGGGTTCTTCCTTTTCCTTTTCCTAACAATATACACAGCAACAAAAGCTCCAGACCTCATGGATCTGTCTTCCCTGCAACGTTTGTCTGACATGATTAGAAGAGCCGATTTGCACAAAATTCATCCAGAATTGTTGAATTGCCTTCCTTCACTAGCTTTTCCATCTATGGATTTTCTCTCCTCAGTTTCAGGGAATATAAGGGAACTTCTCGCCAATTGCTTGCCTGACAGGTTCTCCCACGCCAATCAAACAGAGAATTCTATTCTGCATGGAGTAACAGATGACGTGATGAACATGGTGGCCCCCCTTATGAATCGATCCATAACAAGGTGGCCCTTCTTTGCATTCCTAGGTGGAGCCATGTTCTGCTTGCTAGCCAGCAGTACATGTCATCTCCTTGCTTGCCATTCAGCGCGCCTTTCCTACATAGTGCTCAGATGTGACTATGCAGGCATCGCTGCACTCATCACAACCTCATTTTACCCTCCTGTCTACTACTCCTTCATGTGCAACCCCTTCTTTCGTAACCTCTACTTAAGTTTCATAACAGTGCTAGGTATCACCACCATTGTTTTCTCCCTCCTCCCATTTTTTCAAGGACCCAAGTTTCGTGTCTATCGTGCTTCTCTCTTCTTTGTGATGGGTGTGTCAGGTGTCGTGCCTTTAGTTCATAAGCTCATAGTGTTCAGGAATCAGCCAGAGGCCATCCAGACTACCGGCTATGAGGTGTTAATGGGGGTTCTTTATGGACTAGGGGCATTGATTTATGCTACAAGGGTACCGGAGCGGTGGAGACCAGGGAAGTTTGATATTGCTGGCCACAGCCACCAGCTTTTCCATATTTTGGTTGTGGCAGCAGCTTACACTCACTACCAAGCCGGTCTACTGTACCTAAGATGGAGGGACTTGAAGGGTTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04152 ko04211 ko04920 ko04932 map04152 map04211 map04920 map04932
Pfam Domains
Protein Families
HlyIII

Protein Analysis

398

Amino Acids

45.31

Weight (kDa)

8.61

Isoelectric Point (pI)

47.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HlyIII PF03006 77 - 382 5.4e-62 Haemolysin-III related
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 202
Acc36I ACCTGC 1 cut(s) 202
Acc65I GGTACC 1 cut(s) 1051
AccB1I GGYRCC 1 cut(s) 1051
AccB7I CCANNNNNTGG 1 cut(s) 1117
AccBSI CCGCTC 1 cut(s) 1060
AccI GTMKAC 2 cut(s) 765, 1156
AccII CGCG 1 cut(s) 688
AciI CCGC 1 cut(s) 1060
AclI AACGTT 1 cut(s) 352
AclWI GGATC 2 cut(s) 342, 585
AcoI YGGCCR 2 cut(s) 200, 1090
AcsI RAATTY 2 cut(s) 390, 529
AfaI GTAC 3 cut(s) 658, 1053, 1163
AfiI CCNNNNNNNGG 2 cut(s) 600, 1117
AflII CTTAAG 1 cut(s) 808
AflIII ACRYGT 1 cut(s) 659
AgsI TTSAA 5 cut(s) 134, 208, 409, 869, 1183
AjiI CACGTC 1 cut(s) 559
AjnI CCWGG 2 cut(s) 148, 1069
AluBI AGCT 6 cut(s) 127, 320, 428, 949, 1105, 1133
AluI AGCT 6 cut(s) 127, 320, 428, 949, 1105, 1133
Alw21I GWGCWC 1 cut(s) 708
Alw26I GTCTC 4 cut(s) 94, 156, 239, 1060
AlwI GGATC 2 cut(s) 342, 585
AoxI GGCC 5 cut(s) 200, 573, 605, 975, 1090
ApeKI GCWGC 3 cut(s) 731, 1127, 1130
ApoI RAATTY 2 cut(s) 390, 529
ArsI GACNNNNNNTTYG 2 cut(s) 864, 896
Asp718I GGTACC 1 cut(s) 1051
AspA2I CCTAGG 1 cut(s) 622
AspLEI GCGC 2 cut(s) 688, 690
AspS9I GGNCC 3 cut(s) 574, 606, 872
AsuHPI GGTGA 2 cut(s) 20, 826
AsuNHI GCTAGC 1 cut(s) 646
AvaII GGWCC 1 cut(s) 872
AvrII CCTAGG 1 cut(s) 622
BaeI ACNNNNGTAYC 2 cut(s) 1035, 1068
BalI TGGCCA 2 cut(s) 202, 1092
BanI GGYRCC 1 cut(s) 1051
BbsI GAAGAC 1 cut(s) 333
Bbv12I GWGCWC 1 cut(s) 708
BbvI GCAGC 3 cut(s) 718, 1139, 1142
BccI CCATC 4 cut(s) 442, 907, 986, 1164
BciT130I CCWGG 2 cut(s) 150, 1071
BcoDI GTCTC 4 cut(s) 94, 156, 239, 1060
BfaI CTAG 5 cut(s) 425, 623, 647, 827, 1025
BfrI CTTAAG 1 cut(s) 808
BfuAI ACCTGC 1 cut(s) 202
BisI GCNGC 3 cut(s) 732, 1128, 1131
BlnI CCTAGG 1 cut(s) 622
BlsI GCNGC 3 cut(s) 733, 1129, 1132
Bme1390I CCNGG 2 cut(s) 150, 1071
Bme18I GGWCC 1 cut(s) 872
BmgBI CACGTC 1 cut(s) 559
BmgT120I GGNCC 3 cut(s) 574, 606, 872
BmiI GGNNCC 4 cut(s) 576, 631, 874, 1053
BmrFI CCNGG 2 cut(s) 150, 1071
BmsI GCATC 1 cut(s) 735
BmtI GCTAGC 1 cut(s) 650
BpiI GAAGAC 1 cut(s) 333
BpmI CTGGAG 1 cut(s) 306
Bsa29I ATCGAT 1 cut(s) 589
BsaI GGTCTC 1 cut(s) 1060
BsaJI CCNNGG 2 cut(s) 622, 1070
BsaWI WCCGGW 1 cut(s) 1054
BsaXI ACNNNNNCTCC 4 cut(s) 304, 334, 491, 521
Bsc4I CCNNNNNNNGG 2 cut(s) 600, 1117
Bse118I RCCGGY 2 cut(s) 989, 1151
Bse3DI GCAATG 1 cut(s) 143
BseBI CCWGG 2 cut(s) 150, 1071
BseCI ATCGAT 1 cut(s) 589
BseDI CCNNGG 2 cut(s) 622, 1070
BseGI GGATG 2 cut(s) 394, 978
BseLI CCNNNNNNNGG 2 cut(s) 600, 1117
BseMI GCAATG 1 cut(s) 143
BseMII CTCAG 2 cut(s) 468, 721
BsePI GCGCGC 1 cut(s) 686
BseRI GAGGAG 2 cut(s) 442, 845
BseXI GCAGC 3 cut(s) 718, 1139, 1142
BsgI GTGCAG 1 cut(s) 717
Bsh1236I CGCG 1 cut(s) 688
BshFI GGCC 5 cut(s) 202, 575, 607, 977, 1092
BshNI GGYRCC 1 cut(s) 1051
BshVI ATCGAT 1 cut(s) 589
BsiHKAI GWGCWC 1 cut(s) 708
BsiSI CCGG 3 cut(s) 990, 1055, 1152
BslFI GGGAC 1 cut(s) 1190
BslI CCNNNNNNNGG 2 cut(s) 600, 1117
BsmAI GTCTC 4 cut(s) 94, 156, 239, 1060
BsmFI GGGAC 1 cut(s) 1190
BsmI GAATGC 1 cut(s) 617
BsnI GGCC 5 cut(s) 202, 575, 607, 977, 1092
Bso31I GGTCTC 1 cut(s) 1060
Bsp1286I GDGCHC 1 cut(s) 708
Bsp143I GATC 5 cut(s) 32, 191, 270, 334, 590
BspACI CCGC 1 cut(s) 1060
BspANI GGCC 5 cut(s) 202, 575, 607, 977, 1092
BspCNI CTCAG 2 cut(s) 467, 720
BspDI ATCGAT 1 cut(s) 589
BspFNI CGCG 1 cut(s) 688
BspLI GGNNCC 4 cut(s) 576, 631, 874, 1053
BspMI ACCTGC 1 cut(s) 202
BspOI GCTAGC 1 cut(s) 650
BspPI GGATC 2 cut(s) 342, 585
BspQI GCTCTTC 1 cut(s) 367
BspT107I GGYRCC 1 cut(s) 1051
BspTI CTTAAG 1 cut(s) 808
BspTNI GGTCTC 1 cut(s) 1060
BsrBI CCGCTC 1 cut(s) 1060
BsrDI GCAATG 1 cut(s) 143
BsrFI RCCGGY 2 cut(s) 989, 1151
BssAI RCCGGY 2 cut(s) 989, 1151
BssECI CCNNGG 2 cut(s) 622, 1070
BssHII GCGCGC 1 cut(s) 686
BssMI GATC 5 cut(s) 32, 191, 270, 334, 590
BssT1I CCWWGG 1 cut(s) 622
Bst2UI CCWGG 2 cut(s) 150, 1071
Bst4CI ACNGT 3 cut(s) 56, 823, 1161
Bst6I CTCTTC 2 cut(s) 367, 908
BstAFI CTTAAG 1 cut(s) 808
BstC8I GCNNGC 8 cut(s) 494, 644, 648, 652, 676, 688, 724, 1090
BstDEI CTNAG 4 cut(s) 221, 454, 707, 1166
BstF5I GGATG 2 cut(s) 394, 978
BstFNI CGCG 1 cut(s) 688
BstHHI GCGC 2 cut(s) 688, 690
BstKTI GATC 5 cut(s) 35, 194, 273, 337, 593
BstMAI GTCTC 4 cut(s) 94, 156, 239, 1060
BstMBI GATC 5 cut(s) 32, 191, 270, 334, 590
BstMWI GCNNNNNNNGC 3 cut(s) 208, 317, 728
BstNI CCWGG 2 cut(s) 150, 1071
BstNSI RCATGY 2 cut(s) 78, 663
BstSCI CCNGG 2 cut(s) 148, 1069
BstUI CGCG 1 cut(s) 688
BstV1I GCAGC 3 cut(s) 718, 1139, 1142
BstV2I GAAGAC 1 cut(s) 333
BstX2I RGATCY 1 cut(s) 334
BstYI RGATCY 1 cut(s) 334
Bsu15I ATCGAT 1 cut(s) 589
BsuRI GGCC 5 cut(s) 202, 575, 607, 977, 1092
BsuTUI ATCGAT 1 cut(s) 589
BtgZI GCGATG 1 cut(s) 712
BtrI CACGTC 1 cut(s) 559
BtsCI GGATG 2 cut(s) 394, 978
BtsIMutI CAGTG 1 cut(s) 828
BveI ACCTGC 1 cut(s) 202
Cac8I GCNNGC 8 cut(s) 494, 644, 648, 652, 676, 688, 724, 1090
CfoI GCGC 2 cut(s) 688, 690
Cfr10I RCCGGY 2 cut(s) 989, 1151
Cfr13I GGNCC 3 cut(s) 574, 606, 872
ClaI ATCGAT 1 cut(s) 589
CseI GACGC 1 cut(s) 271
Csp6I GTAC 3 cut(s) 657, 1052, 1162
CviAII CATG 8 cut(s) 75, 331, 364, 542, 568, 634, 660, 778
CviQI GTAC 3 cut(s) 657, 1052, 1162
DdeI CTNAG 4 cut(s) 221, 454, 707, 1166
DpnI GATC 5 cut(s) 34, 193, 272, 336, 592
DpnII GATC 5 cut(s) 32, 191, 270, 334, 590
DraI TTTAAA 1 cut(s) 81
EaeI YGGCCR 2 cut(s) 200, 1090
Eam1104I CTCTTC 2 cut(s) 367, 908
EarI CTCTTC 2 cut(s) 367, 908
Eco130I CCWWGG 1 cut(s) 622
Eco31I GGTCTC 1 cut(s) 1060
Eco47I GGWCC 1 cut(s) 872
EcoO109I RGGNCCY 1 cut(s) 872
EcoRI GAATTC 1 cut(s) 529
EcoRII CCWGG 2 cut(s) 148, 1069
EcoT14I CCWWGG 1 cut(s) 622
ErhI CCWWGG 1 cut(s) 622
FaeI CATG 8 cut(s) 78, 334, 367, 545, 571, 637, 663, 781
FaqI GGGAC 1 cut(s) 1190
FatI CATG 8 cut(s) 74, 330, 363, 541, 567, 633, 659, 777
FblI GTMKAC 2 cut(s) 765, 1156
Fnu4HI GCNGC 3 cut(s) 732, 1128, 1131
FokI GGATG 2 cut(s) 381, 965
Fsp4HI GCNGC 3 cut(s) 732, 1128, 1131
FspBI CTAG 5 cut(s) 425, 623, 647, 827, 1025
GlaI GCGC 2 cut(s) 687, 689
GluI GCNGC 3 cut(s) 732, 1128, 1131
GsuI CTGGAG 1 cut(s) 306
HaeIII GGCC 5 cut(s) 202, 575, 607, 977, 1092
HapII CCGG 3 cut(s) 990, 1055, 1152
HgaI GACGC 1 cut(s) 271
HhaI GCGC 2 cut(s) 688, 690
Hin1II CATG 8 cut(s) 78, 334, 367, 545, 571, 637, 663, 781
Hin6I GCGC 2 cut(s) 686, 688
HinP1I GCGC 2 cut(s) 686, 688
HinfI GANTC 4 cut(s) 11, 247, 586, 964
HpaII CCGG 3 cut(s) 990, 1055, 1152
HphI GGTGA 2 cut(s) 20, 826
Hpy166II GTNNAC 3 cut(s) 67, 766, 1157
Hpy188I TCNGA 4 cut(s) 25, 196, 361, 710
Hpy188III TCNNGA 5 cut(s) 259, 323, 398, 961, 982
Hpy8I GTNNAC 3 cut(s) 67, 766, 1157
HpyAV CCTTC 6 cut(s) 425, 429, 619, 784, 799, 1177
HpyCH4III ACNGT 3 cut(s) 56, 823, 1161
HpyCH4IV ACGT 2 cut(s) 352, 558
HpyCH4V TGCA 7 cut(s) 349, 385, 541, 617, 722, 734, 783
HpyF10VI GCNNNNNNNGC 3 cut(s) 208, 317, 728
HpyF3I CTNAG 4 cut(s) 221, 454, 707, 1166
HpySE526I ACGT 2 cut(s) 352, 558
Hsp92II CATG 8 cut(s) 78, 334, 367, 545, 571, 637, 663, 781
HspAI GCGC 2 cut(s) 686, 688
KpnI GGTACC 1 cut(s) 1055
Kzo9I GATC 5 cut(s) 32, 191, 270, 334, 590
LguI GCTCTTC 1 cut(s) 367
LmnI GCTCC 3 cut(s) 325, 629, 1057
Lsp1109I GCAGC 3 cut(s) 718, 1139, 1142
LweI GCATC 1 cut(s) 735
MaeI CTAG 5 cut(s) 425, 623, 647, 827, 1025
MaeII ACGT 2 cut(s) 352, 558
MaeIII GTNAC 3 cut(s) 547, 713, 797
MalI GATC 5 cut(s) 34, 193, 272, 336, 592
MbiI CCGCTC 1 cut(s) 1060
MboI GATC 5 cut(s) 32, 191, 270, 334, 590
MboII GAAGA 6 cut(s) 71, 121, 271, 333, 384, 895
MfeI CAATTG 1 cut(s) 487
MflI RGATCY 1 cut(s) 334
MhlI GDGCHC 1 cut(s) 708
MlsI TGGCCA 2 cut(s) 202, 1092
MluCI AATT 6 cut(s) 47, 390, 401, 409, 487, 529
MluNI TGGCCA 2 cut(s) 202, 1092
MlyI GAGTC 1 cut(s) 241
Mox20I TGGCCA 2 cut(s) 202, 1092
MscI TGGCCA 2 cut(s) 202, 1092
MseI TTAA 5 cut(s) 80, 158, 252, 809, 1004
MslI CAYNNNNRTG 1 cut(s) 821
Msp20I TGGCCA 2 cut(s) 202, 1092
MspCI CTTAAG 1 cut(s) 808
MspI CCGG 3 cut(s) 990, 1055, 1152
MspR9I CCNGG 2 cut(s) 150, 1071
MunI CAATTG 1 cut(s) 487
Mva1269I GAATGC 1 cut(s) 617
MvaI CCWGG 2 cut(s) 150, 1071
MvnI CGCG 1 cut(s) 688
MwoI GCNNNNNNNGC 3 cut(s) 208, 317, 728
NdeII GATC 5 cut(s) 32, 191, 270, 334, 590
NheI GCTAGC 1 cut(s) 646
NlaIII CATG 8 cut(s) 78, 334, 367, 545, 571, 637, 663, 781
NlaIV GGNNCC 4 cut(s) 576, 631, 874, 1053
NmuCI GTSAC 1 cut(s) 713
NspI RCATGY 2 cut(s) 78, 663
PaqCI CACCTGC 1 cut(s) 202
PauI GCGCGC 1 cut(s) 686
PciI ACATGT 1 cut(s) 659
PciSI GCTCTTC 1 cut(s) 367
PcsI WCGNNNNNNNCGW 1 cut(s) 889
PctI GAATGC 1 cut(s) 617
PfeI GAWTC 3 cut(s) 11, 586, 964
PflMI CCANNNNNTGG 1 cut(s) 1117
PkrI GCNGC 3 cut(s) 733, 1129, 1132
PleI GAGTC 1 cut(s) 241
PpsI GAGTC 1 cut(s) 241
PpuMI RGGWCCY 1 cut(s) 872
PscI ACATGT 1 cut(s) 659
Psp1406I AACGTT 1 cut(s) 352
Psp5II RGGWCCY 1 cut(s) 872
Psp6I CCWGG 2 cut(s) 148, 1069
PspGI CCWGG 2 cut(s) 148, 1069
PspN4I GGNNCC 4 cut(s) 576, 631, 874, 1053
PspPI GGNCC 3 cut(s) 574, 606, 872
PspPPI RGGWCCY 1 cut(s) 872
PsuI RGATCY 1 cut(s) 334
PteI GCGCGC 1 cut(s) 686
RsaI GTAC 3 cut(s) 658, 1053, 1163
RsaNI GTAC 3 cut(s) 657, 1052, 1162
RseI CAYNNNNRTG 1 cut(s) 821
SapI GCTCTTC 1 cut(s) 367
SaqAI TTAA 5 cut(s) 80, 158, 252, 809, 1004
SatI GCNGC 3 cut(s) 732, 1128, 1131
Sau3AI GATC 5 cut(s) 32, 191, 270, 334, 590
Sau96I GGNCC 3 cut(s) 574, 606, 872
SchI GAGTC 1 cut(s) 241
ScrFI CCNGG 2 cut(s) 150, 1071
SduI GDGCHC 1 cut(s) 708
SfaNI GCATC 1 cut(s) 735
SinI GGWCC 1 cut(s) 872
SmiMI CAYNNNNRTG 1 cut(s) 821
SmlI CTYRAG 1 cut(s) 808
SmoI CTYRAG 1 cut(s) 808
Sse9I AATT 6 cut(s) 47, 390, 401, 409, 487, 529
SsiI CCGC 1 cut(s) 1060
SspMI CTAG 5 cut(s) 425, 623, 647, 827, 1025
StyD4I CCNGG 2 cut(s) 148, 1069
StyI CCWWGG 1 cut(s) 622
TaaI ACNGT 3 cut(s) 56, 823, 1161
TaiI ACGT 2 cut(s) 355, 561
TaqI TCGA 1 cut(s) 589
TasI AATT 6 cut(s) 47, 390, 401, 409, 487, 529
TatI WGTACW 1 cut(s) 656
TfiI GAWTC 3 cut(s) 11, 586, 964
Tru1I TTAA 5 cut(s) 80, 158, 252, 809, 1004
Tru9I TTAA 5 cut(s) 80, 158, 252, 809, 1004
TscAI CASTG 1 cut(s) 828
TseFI GTSAC 1 cut(s) 713
TseI GCWGC 3 cut(s) 731, 1127, 1130
Tsp45I GTSAC 1 cut(s) 713
TspDTI ATGAA 9 cut(s) 132, 163, 227, 383, 578, 599, 766, 805, 932
TspRI CASTG 1 cut(s) 828
Van91I CCANNNNNTGG 1 cut(s) 1117
Vha464I CTTAAG 1 cut(s) 808
VpaK11BI GGWCC 1 cut(s) 872
XapI RAATTY 2 cut(s) 390, 529
XceI RCATGY 2 cut(s) 78, 663
XcmI CCANNNNNNNNNTGG 1 cut(s) 146
XmaJI CCTAGG 1 cut(s) 622
XmiI GTMKAC 2 cut(s) 765, 1156
XspI CTAG 5 cut(s) 425, 623, 647, 827, 1025
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.