pycom04g04650

Haemolysin-III related

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0001650
Physical Location & Seq
Reverse (-)
4258960 .. 4260033
1074 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g04650.2

Sequence Viewer

Length: 831 bp
ATGATTAGAAGAGCCAATTTGCACAGAATTCATCCGGAGCTGTTGAATTGCCTCCCTTCGCTATCTTTGCCGTCTATGAATTTTCTCTCCTCGGTTTCAAGGAATATAAGTGAACTTCTCGCCAATTGCTTGCCTGACCGGGTCTCCCATGGCAATCAAACAGAGAATTCTGTTCTGGATGGAGTAACGGATGACGTGATGAACATGGTGGCCCCTCTTATGTATCGGCCCATAACAAGGTGGCCCTTCTTTGTGTTCCTAGGTGGAGCCATGTTCTGCTTGCTAGCCAGCAGCACATGCCATCTCCTTACTTGCCATTCAGCGCGCCTTTCCTACATAGTGCACAGATGTGACTATGCAGGCATCGCTGCACTCATCGTGACCTCATTTTACCCTCCTGTCTACTACTCCTTCATGTGCAACCCCTTCCTTCGCAACCTCTACTTAGGTTTCATCACAATACTAGGTATCACCACCATGGTTTTCTCCCTCCTCCCATTTTTCCAAGGACCCAAGTTTCGAAGCCATCGTGCCTCTCTCTTCTTTGGGATGGGCGTGTCAGGTGTCGTGCCTTTAGTTCATAAGCTCATAGTGTTCAGGAATCAGCCAGAGGCCATCCAGACTACCGGCTATGAGGTGTTAATGGGGGTTCTTTATGGACTAGGGGCATTGATATATGCTATAAGGATACCCGAGCGGTGGAGACCGGGGAAGTTTGATATTGCTGGGAACAGTCACCAGCTTTTCCATATCTTGGTTGTGGCAGCAGCTTACACACACTACCGTGCCGGTCTACTGTACCTAAGATGGAGGGACTTGAAGGGTTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04152 ko04211 ko04920 ko04932 map04152 map04211 map04920 map04932
Pfam Domains
Protein Families
HlyIII

Protein Analysis

277

Amino Acids

31.14

Weight (kDa)

9.38

Isoelectric Point (pI)

47.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 754
AccBSI CCGCTC 1 cut(s) 697
AccI GTMKAC 2 cut(s) 402, 793
AccII CGCG 1 cut(s) 325
AccIII TCCGGA 1 cut(s) 34
AciI CCGC 1 cut(s) 697
AcsI RAATTY 3 cut(s) 27, 79, 166
AfaI GTAC 1 cut(s) 800
AfiI CCNNNNNNNGG 3 cut(s) 237, 699, 754
AgsI TTSAA 3 cut(s) 46, 99, 820
AjiI CACGTC 1 cut(s) 196
AleI CACNNNNGTG 2 cut(s) 348, 783
AluBI AGCT 4 cut(s) 40, 586, 742, 770
AluI AGCT 4 cut(s) 40, 586, 742, 770
Alw21I GWGCWC 1 cut(s) 345
Alw26I GTCTC 2 cut(s) 148, 697
Alw44I GTGCAC 1 cut(s) 341
Ama87I CYCGRG 1 cut(s) 692
Aor13HI TCCGGA 1 cut(s) 34
AoxI GGCC 4 cut(s) 210, 227, 242, 612
ApaLI GTGCAC 1 cut(s) 341
ApeKI GCWGC 4 cut(s) 291, 368, 764, 767
ApoI RAATTY 3 cut(s) 27, 79, 166
ArsI GACNNNNNNTTYG 2 cut(s) 501, 533
AspA2I CCTAGG 1 cut(s) 259
AspLEI GCGC 2 cut(s) 325, 327
AspS9I GGNCC 4 cut(s) 211, 228, 243, 509
AsuC2I CCSGG 2 cut(s) 140, 708
AsuHPI GGTGA 2 cut(s) 463, 728
AsuII TTCGAA 1 cut(s) 520
AsuNHI GCTAGC 1 cut(s) 283
AvaI CYCGRG 1 cut(s) 692
AvaII GGWCC 1 cut(s) 509
AvrII CCTAGG 1 cut(s) 259
BaeGI GKGCMC 1 cut(s) 345
Bbv12I GWGCWC 1 cut(s) 345
BbvI GCAGC 4 cut(s) 303, 355, 776, 779
BccI CCATC 6 cut(s) 173, 309, 534, 544, 623, 801
BceAI ACGGC 1 cut(s) 55
BciVI GTATCC 1 cut(s) 681
BcnI CCSGG 2 cut(s) 140, 708
BcoDI GTCTC 2 cut(s) 148, 697
BfaI CTAG 4 cut(s) 260, 284, 464, 662
BfuI GTATCC 1 cut(s) 681
BisI GCNGC 4 cut(s) 292, 369, 765, 768
BlnI CCTAGG 1 cut(s) 259
BlsI GCNGC 4 cut(s) 293, 370, 766, 769
Bme1390I CCNGG 2 cut(s) 140, 708
Bme18I GGWCC 1 cut(s) 509
BmeT110I CYCGRG 1 cut(s) 692
BmgBI CACGTC 1 cut(s) 196
BmgT120I GGNCC 4 cut(s) 211, 228, 243, 509
BmiI GGNNCC 3 cut(s) 213, 268, 511
BmrFI CCNGG 2 cut(s) 140, 708
BmsI GCATC 1 cut(s) 372
BmtI GCTAGC 1 cut(s) 287
Bpu14I TTCGAA 1 cut(s) 520
BpuMI CCSGG 2 cut(s) 140, 708
BsaI GGTCTC 2 cut(s) 148, 697
BsaJI CCNNGG 6 cut(s) 90, 148, 259, 477, 505, 707
BsaWI WCCGGW 1 cut(s) 34
BsaXI ACNNNNNCTCC 2 cut(s) 128, 158
Bsc4I CCNNNNNNNGG 3 cut(s) 237, 699, 754
Bse118I RCCGGY 2 cut(s) 626, 788
BseAI TCCGGA 1 cut(s) 34
BseDI CCNNGG 6 cut(s) 90, 148, 259, 477, 505, 707
BseGI GGATG 5 cut(s) 31, 184, 196, 555, 615
BseLI CCNNNNNNNGG 3 cut(s) 237, 699, 754
BsePI GCGCGC 1 cut(s) 323
BseRI GAGGAG 2 cut(s) 79, 482
BseSI GKGCMC 1 cut(s) 345
BseXI GCAGC 4 cut(s) 303, 355, 776, 779
BseYI CCCAGC 1 cut(s) 725
BsgI GTGCAG 1 cut(s) 354
Bsh1236I CGCG 1 cut(s) 325
BshFI GGCC 4 cut(s) 212, 229, 244, 614
BsiHKAI GWGCWC 1 cut(s) 345
BsiHKCI CYCGRG 1 cut(s) 692
BsiSI CCGG 5 cut(s) 35, 139, 627, 707, 789
BslFI GGGAC 1 cut(s) 827
BslI CCNNNNNNNGG 3 cut(s) 237, 699, 754
BsmAI GTCTC 2 cut(s) 148, 697
BsmFI GGGAC 1 cut(s) 827
BsnI GGCC 4 cut(s) 212, 229, 244, 614
Bso31I GGTCTC 2 cut(s) 148, 697
BsoBI CYCGRG 1 cut(s) 692
Bsp119I TTCGAA 1 cut(s) 520
Bsp1286I GDGCHC 1 cut(s) 345
Bsp13I TCCGGA 1 cut(s) 34
Bsp19I CCATGG 2 cut(s) 148, 477
BspACI CCGC 1 cut(s) 697
BspANI GGCC 4 cut(s) 212, 229, 244, 614
BspEI TCCGGA 1 cut(s) 34
BspFNI CGCG 1 cut(s) 325
BspLI GGNNCC 3 cut(s) 213, 268, 511
BspOI GCTAGC 1 cut(s) 287
BspQI GCTCTTC 1 cut(s) 4
BspT104I TTCGAA 1 cut(s) 520
BspTNI GGTCTC 2 cut(s) 148, 697
BsrBI CCGCTC 1 cut(s) 697
BsrFI RCCGGY 2 cut(s) 626, 788
BssAI RCCGGY 2 cut(s) 626, 788
BssECI CCNNGG 6 cut(s) 90, 148, 259, 477, 505, 707
BssHII GCGCGC 1 cut(s) 323
BssT1I CCWWGG 4 cut(s) 148, 259, 477, 505
Bst4CI ACNGT 3 cut(s) 734, 785, 798
Bst6I CTCTTC 2 cut(s) 4, 545
BstAPI GCANNNNNTGC 1 cut(s) 297
BstBI TTCGAA 1 cut(s) 520
BstC8I GCNNGC 6 cut(s) 131, 281, 285, 289, 325, 361
BstDEI CTNAG 2 cut(s) 445, 803
BstDSI CCRYGG 2 cut(s) 148, 477
BstF5I GGATG 5 cut(s) 31, 184, 196, 555, 615
BstFNI CGCG 1 cut(s) 325
BstHHI GCGC 2 cut(s) 325, 327
BstMAI GTCTC 2 cut(s) 148, 697
BstMWI GCNNNNNNNGC 3 cut(s) 67, 297, 365
BstNSI RCATGY 1 cut(s) 300
BstSCI CCNGG 2 cut(s) 138, 706
BstSLI GKGCMC 1 cut(s) 345
BstUI CGCG 1 cut(s) 325
BstV1I GCAGC 4 cut(s) 303, 355, 776, 779
BsuI GTATCC 1 cut(s) 681
BsuRI GGCC 4 cut(s) 212, 229, 244, 614
BtgI CCRYGG 2 cut(s) 148, 477
BtgZI GCGATG 1 cut(s) 349
BtrI CACGTC 1 cut(s) 196
BtsCI GGATG 5 cut(s) 31, 184, 196, 555, 615
Cac8I GCNNGC 6 cut(s) 131, 281, 285, 289, 325, 361
CfoI GCGC 2 cut(s) 325, 327
Cfr10I RCCGGY 2 cut(s) 626, 788
Cfr13I GGNCC 4 cut(s) 211, 228, 243, 509
Csp6I GTAC 1 cut(s) 799
CviAII CATG 6 cut(s) 149, 205, 271, 297, 415, 478
CviQI GTAC 1 cut(s) 799
DdeI CTNAG 2 cut(s) 445, 803
Eam1104I CTCTTC 2 cut(s) 4, 545
EarI CTCTTC 2 cut(s) 4, 545
Eco130I CCWWGG 4 cut(s) 148, 259, 477, 505
Eco31I GGTCTC 2 cut(s) 148, 697
Eco47I GGWCC 1 cut(s) 509
Eco88I CYCGRG 1 cut(s) 692
EcoO109I RGGNCCY 1 cut(s) 509
EcoRI GAATTC 2 cut(s) 27, 166
EcoT14I CCWWGG 4 cut(s) 148, 259, 477, 505
ErhI CCWWGG 4 cut(s) 148, 259, 477, 505
FaeI CATG 6 cut(s) 152, 208, 274, 300, 418, 481
FaqI GGGAC 1 cut(s) 827
FatI CATG 6 cut(s) 148, 204, 270, 296, 414, 477
FblI GTMKAC 2 cut(s) 402, 793
Fnu4HI GCNGC 4 cut(s) 292, 369, 765, 768
FokI GGATG 5 cut(s) 18, 191, 203, 562, 602
Fsp4HI GCNGC 4 cut(s) 292, 369, 765, 768
FspBI CTAG 4 cut(s) 260, 284, 464, 662
GlaI GCGC 2 cut(s) 324, 326
GluI GCNGC 4 cut(s) 292, 369, 765, 768
GsaI CCCAGC 1 cut(s) 729
HaeIII GGCC 4 cut(s) 212, 229, 244, 614
HapII CCGG 5 cut(s) 35, 139, 627, 707, 789
HhaI GCGC 2 cut(s) 325, 327
Hin1II CATG 6 cut(s) 152, 208, 274, 300, 418, 481
Hin6I GCGC 2 cut(s) 323, 325
HinP1I GCGC 2 cut(s) 323, 325
HinfI GANTC 1 cut(s) 601
HpaII CCGG 5 cut(s) 35, 139, 627, 707, 789
HphI GGTGA 2 cut(s) 463, 728
Hpy166II GTNNAC 4 cut(s) 113, 343, 403, 794
Hpy188III TCNNGA 5 cut(s) 35, 176, 379, 598, 619
Hpy8I GTNNAC 4 cut(s) 113, 343, 403, 794
HpyAV CCTTC 6 cut(s) 66, 256, 421, 436, 440, 814
HpyCH4III ACNGT 3 cut(s) 734, 785, 798
HpyCH4IV ACGT 1 cut(s) 195
HpyCH4V TGCA 5 cut(s) 22, 343, 359, 371, 420
HpyF10VI GCNNNNNNNGC 3 cut(s) 67, 297, 365
HpyF3I CTNAG 2 cut(s) 445, 803
HpySE526I ACGT 1 cut(s) 195
Hsp92II CATG 6 cut(s) 152, 208, 274, 300, 418, 481
HspAI GCGC 2 cut(s) 323, 325
Kpn2I TCCGGA 1 cut(s) 34
LguI GCTCTTC 1 cut(s) 4
LmnI GCTCC 2 cut(s) 37, 266
Lsp1109I GCAGC 4 cut(s) 303, 355, 776, 779
LweI GCATC 1 cut(s) 372
MaeI CTAG 4 cut(s) 260, 284, 464, 662
MaeII ACGT 1 cut(s) 195
MaeIII GTNAC 4 cut(s) 184, 350, 379, 734
MbiI CCGCTC 1 cut(s) 697
MboII GAAGA 2 cut(s) 21, 532
MfeI CAATTG 1 cut(s) 124
MhlI GDGCHC 1 cut(s) 345
MluCI AATT 6 cut(s) 16, 27, 46, 79, 124, 166
MroI TCCGGA 1 cut(s) 34
MseI TTAA 1 cut(s) 641
MslI CAYNNNNRTG 3 cut(s) 348, 476, 783
MspI CCGG 5 cut(s) 35, 139, 627, 707, 789
MspR9I CCNGG 2 cut(s) 140, 708
MunI CAATTG 1 cut(s) 124
MvnI CGCG 1 cut(s) 325
MwoI GCNNNNNNNGC 3 cut(s) 67, 297, 365
NciI CCSGG 2 cut(s) 140, 708
NcoI CCATGG 2 cut(s) 148, 477
NheI GCTAGC 1 cut(s) 283
NlaIII CATG 6 cut(s) 152, 208, 274, 300, 418, 481
NlaIV GGNNCC 3 cut(s) 213, 268, 511
NmuCI GTSAC 3 cut(s) 350, 379, 734
NspI RCATGY 1 cut(s) 300
NspV TTCGAA 1 cut(s) 520
OliI CACNNNNGTG 2 cut(s) 348, 783
PauI GCGCGC 1 cut(s) 323
PciSI GCTCTTC 1 cut(s) 4
PcsI WCGNNNNNNNCGW 1 cut(s) 526
PfeI GAWTC 1 cut(s) 601
PflFI GACNNNGTC 1 cut(s) 140
PflMI CCANNNNNTGG 1 cut(s) 754
PkrI GCNGC 4 cut(s) 293, 370, 766, 769
PpuMI RGGWCCY 1 cut(s) 509
Psp5II RGGWCCY 1 cut(s) 509
PspFI CCCAGC 1 cut(s) 725
PspN4I GGNNCC 3 cut(s) 213, 268, 511
PspPI GGNCC 4 cut(s) 211, 228, 243, 509
PspPPI RGGWCCY 1 cut(s) 509
PsyI GACNNNGTC 1 cut(s) 140
PteI GCGCGC 1 cut(s) 323
RsaI GTAC 1 cut(s) 800
RsaNI GTAC 1 cut(s) 799
RseI CAYNNNNRTG 3 cut(s) 348, 476, 783
SapI GCTCTTC 1 cut(s) 4
SaqAI TTAA 1 cut(s) 641
SatI GCNGC 4 cut(s) 292, 369, 765, 768
Sau96I GGNCC 4 cut(s) 211, 228, 243, 509
ScrFI CCNGG 2 cut(s) 140, 708
SduI GDGCHC 1 cut(s) 345
SfaNI GCATC 1 cut(s) 372
SfuI TTCGAA 1 cut(s) 520
SinI GGWCC 1 cut(s) 509
SmiMI CAYNNNNRTG 3 cut(s) 348, 476, 783
Sse9I AATT 6 cut(s) 16, 27, 46, 79, 124, 166
SsiI CCGC 1 cut(s) 697
SspMI CTAG 4 cut(s) 260, 284, 464, 662
StyD4I CCNGG 2 cut(s) 138, 706
StyI CCWWGG 4 cut(s) 148, 259, 477, 505
TaaI ACNGT 3 cut(s) 734, 785, 798
TaiI ACGT 1 cut(s) 198
TaqI TCGA 1 cut(s) 520
TasI AATT 6 cut(s) 16, 27, 46, 79, 124, 166
TfiI GAWTC 1 cut(s) 601
Tru1I TTAA 1 cut(s) 641
Tru9I TTAA 1 cut(s) 641
TseFI GTSAC 3 cut(s) 350, 379, 734
TseI GCWGC 4 cut(s) 291, 368, 764, 767
Tsp45I GTSAC 3 cut(s) 350, 379, 734
TspDTI ATGAA 6 cut(s) 20, 92, 215, 403, 442, 569
TspGWI ACGGA 1 cut(s) 203
Tth111I GACNNNGTC 1 cut(s) 140
Van91I CCANNNNNTGG 1 cut(s) 754
VneI GTGCAC 1 cut(s) 341
VpaK11BI GGWCC 1 cut(s) 509
XapI RAATTY 3 cut(s) 27, 79, 166
XceI RCATGY 1 cut(s) 300
XmaJI CCTAGG 1 cut(s) 259
XmiI GTMKAC 2 cut(s) 402, 793
XspI CTAG 4 cut(s) 260, 284, 464, 662
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.