MD04G1053000.v1.1

Haemolysin-III related

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0001650
Physical Location & Seq
Reverse (-)
6229420 .. 6232716
3297 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1053000.v1.1.491

Sequence Viewer

Length: 1194 bp
ATGGGTGCTGACTCTCCATTATCAGAAACAAGATCAGTAGAAAATCAATTTGACCGTGAAGATGTAAACGAAACGCGTTCAAAGGAAGCGAAGGGAAAGAGACTCTGGAAGAAAGTTAAATATCAGCTTATTGAATACCATTCATTGCCTTATTTTTTAAGGGACAATGAGTTCATTTTGGGTCATTACCGATCGGAATGGTCATTGAAGCAGGTGTTTCTAAGCATCTTCTCCATTCATAATGAGACTCTTAATGTCTGGACGCATTTGATCGGGTTCTTCCTTTTCCTTTTCCTAACCATATACACAGCAACAAAAGCTCCAGACCTCATGGATCTGTCTTCCCTGCAACGTTTGTCTGACATGATTAGAAGAGCCGATTTGCACAGAATTCATCCAGAACTGTTGAATTGCCTCCCTTCGCTATCTTTGCCGTCTATGGATTTTCTCTCCTCAGTTTCAGGGAATATAAGGGAACTTCTCGCCAATTGCTTGCCTGACCGGTTCTCCCATGGCAATCAAACAGAGAATTCTGTTCTGGATGGAGTAACGGATGACGTGATGAACATGGTGGCCCCCCTTATGTATCGGCCCATAACAAGGTGGCCCTTCTTTGTGTTCCTAGGTGGAGCCATGTTCTGCTTGCTAGCCAGCAGCACATGCCATCTCCTTAGTTGCCATTCAGCGCGCCTTTCCTACATAGTGCACAGATGTGACTATGCAGGCATCGCTGCACTCATCGTGACCTCATTTTACCCTCCTGTCTACTACTCCTTCATGTGCAACCCCTTCCTTTGCAACCTCTACTTAGGTTTCATCACAATACTAGGTATCACCACCATGGTTTTCTCCCTCCTCCCATTTTTCCAAGGAGCCAAGTTTCGTAGCCATCGTGCTTCTCTCTTCTTTGGGATGGGCGTGTCAGGTGTTGTGCCTTTAGTTCATAAGCTCATAGTGTTCAGGAATCAGCCAGAGGCCATCCAGACAACCGGCTATGAGGTGTTAATGGGGGTTCTTTATGGACTAGGGGCATTGATTTATGCTACAAGGATACCCGAGCGGTGGAGACCAGGGAAGTTTGATATTGCTGGGAACAGTCACCAACTTTTCCATATCTTGGTTGTGGCAGCAGCTTACACACACTACCGTGCCGGTCTACTGTACCTAAGATGGAGGGACTTGAAGGGTTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04152 ko04211 ko04920 ko04932 map04152 map04211 map04920 map04932
Pfam Domains
Protein Families
HlyIII

Protein Analysis

398

Amino Acids

45.39

Weight (kDa)

8.77

Isoelectric Point (pI)

45.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HlyIII PF03006 77 - 382 1.3e-60 Haemolysin-III related
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 202
Acc36I ACCTGC 1 cut(s) 202
AccB7I CCANNNNNTGG 1 cut(s) 1117
AccBSI CCGCTC 1 cut(s) 1060
AccI GTMKAC 2 cut(s) 765, 1156
AccII CGCG 2 cut(s) 76, 688
AciI CCGC 1 cut(s) 1060
AclI AACGTT 1 cut(s) 352
AclWI GGATC 1 cut(s) 342
AcsI RAATTY 2 cut(s) 390, 529
AfaI GTAC 1 cut(s) 1163
AfiI CCNNNNNNNGG 3 cut(s) 600, 1062, 1117
AflIII ACRYGT 1 cut(s) 74
AgeI ACCGGT 1 cut(s) 501
AgsI TTSAA 5 cut(s) 81, 134, 208, 409, 1183
AjiI CACGTC 1 cut(s) 559
AjnI CCWGG 1 cut(s) 1069
AleI CACNNNNGTG 2 cut(s) 711, 1146
AloI GAACNNNNNNTCC 2 cut(s) 155, 187
AluBI AGCT 4 cut(s) 127, 320, 949, 1133
AluI AGCT 4 cut(s) 127, 320, 949, 1133
Alw21I GWGCWC 1 cut(s) 708
Alw26I GTCTC 3 cut(s) 94, 239, 1060
Alw44I GTGCAC 1 cut(s) 704
AlwI GGATC 1 cut(s) 342
Ama87I CYCGRG 1 cut(s) 1055
AoxI GGCC 4 cut(s) 573, 590, 605, 975
ApaLI GTGCAC 1 cut(s) 704
ApeKI GCWGC 4 cut(s) 654, 731, 1127, 1130
ApoI RAATTY 2 cut(s) 390, 529
AsiGI ACCGGT 1 cut(s) 501
AspA2I CCTAGG 1 cut(s) 622
AspLEI GCGC 2 cut(s) 688, 690
AspS9I GGNCC 3 cut(s) 574, 591, 606
AsuHPI GGTGA 2 cut(s) 826, 1091
AsuNHI GCTAGC 1 cut(s) 646
AvaI CYCGRG 1 cut(s) 1055
AvrII CCTAGG 1 cut(s) 622
BaeGI GKGCMC 1 cut(s) 708
BbsI GAAGAC 1 cut(s) 333
Bbv12I GWGCWC 1 cut(s) 708
BbvI GCAGC 4 cut(s) 666, 718, 1139, 1142
BccI CCATC 6 cut(s) 536, 672, 897, 907, 986, 1164
BceAI ACGGC 1 cut(s) 418
BciT130I CCWGG 1 cut(s) 1071
BciVI GTATCC 1 cut(s) 1044
BcoDI GTCTC 3 cut(s) 94, 239, 1060
BfaI CTAG 4 cut(s) 623, 647, 827, 1025
BfuAI ACCTGC 1 cut(s) 202
BfuI GTATCC 1 cut(s) 1044
BisI GCNGC 4 cut(s) 655, 732, 1128, 1131
BlnI CCTAGG 1 cut(s) 622
BlsI GCNGC 4 cut(s) 656, 733, 1129, 1132
Bme1390I CCNGG 1 cut(s) 1071
BmeT110I CYCGRG 1 cut(s) 1055
BmgBI CACGTC 1 cut(s) 559
BmgT120I GGNCC 3 cut(s) 574, 591, 606
BmiI GGNNCC 3 cut(s) 576, 631, 874
BmrFI CCNGG 1 cut(s) 1071
BmsI GCATC 2 cut(s) 234, 735
BmtI GCTAGC 1 cut(s) 650
BpiI GAAGAC 1 cut(s) 333
BpmI CTGGAG 1 cut(s) 306
BsaI GGTCTC 1 cut(s) 1060
BsaJI CCNNGG 5 cut(s) 511, 622, 840, 868, 1070
BsaWI WCCGGW 1 cut(s) 501
BsaXI ACNNNNNCTCC 4 cut(s) 304, 334, 491, 521
Bsc4I CCNNNNNNNGG 3 cut(s) 600, 1062, 1117
Bse118I RCCGGY 3 cut(s) 501, 989, 1151
Bse3DI GCAATG 1 cut(s) 143
BseBI CCWGG 1 cut(s) 1071
BseDI CCNNGG 5 cut(s) 511, 622, 840, 868, 1070
BseGI GGATG 5 cut(s) 394, 547, 559, 918, 978
BseLI CCNNNNNNNGG 3 cut(s) 600, 1062, 1117
BseMI GCAATG 1 cut(s) 143
BseMII CTCAG 1 cut(s) 468
BsePI GCGCGC 1 cut(s) 686
BseRI GAGGAG 2 cut(s) 442, 845
BseSI GKGCMC 1 cut(s) 708
BseXI GCAGC 4 cut(s) 666, 718, 1139, 1142
BseYI CCCAGC 1 cut(s) 1088
BsgI GTGCAG 1 cut(s) 717
Bsh1236I CGCG 2 cut(s) 76, 688
Bsh1285I CGRYCG 1 cut(s) 194
BshFI GGCC 4 cut(s) 575, 592, 607, 977
BshTI ACCGGT 1 cut(s) 501
BsiEI CGRYCG 1 cut(s) 194
BsiHKAI GWGCWC 1 cut(s) 708
BsiHKCI CYCGRG 1 cut(s) 1055
BsiSI CCGG 3 cut(s) 502, 990, 1152
BslFI GGGAC 2 cut(s) 176, 1190
BslI CCNNNNNNNGG 3 cut(s) 600, 1062, 1117
BsmAI GTCTC 3 cut(s) 94, 239, 1060
BsmFI GGGAC 2 cut(s) 176, 1190
BsnI GGCC 4 cut(s) 575, 592, 607, 977
Bso31I GGTCTC 1 cut(s) 1060
BsoBI CYCGRG 1 cut(s) 1055
Bsp1286I GDGCHC 1 cut(s) 708
Bsp143I GATC 4 cut(s) 32, 191, 270, 334
Bsp19I CCATGG 2 cut(s) 511, 840
BspACI CCGC 1 cut(s) 1060
BspANI GGCC 4 cut(s) 575, 592, 607, 977
BspCNI CTCAG 1 cut(s) 467
BspFNI CGCG 2 cut(s) 76, 688
BspLI GGNNCC 3 cut(s) 576, 631, 874
BspMI ACCTGC 1 cut(s) 202
BspOI GCTAGC 1 cut(s) 650
BspPI GGATC 1 cut(s) 342
BspQI GCTCTTC 1 cut(s) 367
BspTNI GGTCTC 1 cut(s) 1060
BsrBI CCGCTC 1 cut(s) 1060
BsrDI GCAATG 1 cut(s) 143
BsrFI RCCGGY 3 cut(s) 501, 989, 1151
BssAI RCCGGY 3 cut(s) 501, 989, 1151
BssECI CCNNGG 5 cut(s) 511, 622, 840, 868, 1070
BssHII GCGCGC 1 cut(s) 686
BssMI GATC 4 cut(s) 32, 191, 270, 334
BssT1I CCWWGG 4 cut(s) 511, 622, 840, 868
Bst2UI CCWGG 1 cut(s) 1071
Bst4CI ACNGT 5 cut(s) 56, 405, 1097, 1148, 1161
Bst6I CTCTTC 2 cut(s) 367, 908
BstAPI GCANNNNNTGC 1 cut(s) 660
BstC8I GCNNGC 6 cut(s) 494, 644, 648, 652, 688, 724
BstDEI CTNAG 5 cut(s) 221, 454, 671, 808, 1166
BstDSI CCRYGG 2 cut(s) 511, 840
BstF5I GGATG 5 cut(s) 394, 547, 559, 918, 978
BstFNI CGCG 2 cut(s) 76, 688
BstHHI GCGC 2 cut(s) 688, 690
BstKTI GATC 4 cut(s) 35, 194, 273, 337
BstMAI GTCTC 3 cut(s) 94, 239, 1060
BstMBI GATC 4 cut(s) 32, 191, 270, 334
BstMCI CGRYCG 1 cut(s) 194
BstMWI GCNNNNNNNGC 4 cut(s) 317, 430, 660, 728
BstNI CCWGG 1 cut(s) 1071
BstNSI RCATGY 1 cut(s) 663
BstSCI CCNGG 1 cut(s) 1069
BstSLI GKGCMC 1 cut(s) 708
BstUI CGCG 2 cut(s) 76, 688
BstV1I GCAGC 4 cut(s) 666, 718, 1139, 1142
BstV2I GAAGAC 1 cut(s) 333
BstX2I RGATCY 1 cut(s) 334
BstYI RGATCY 1 cut(s) 334
BsuI GTATCC 1 cut(s) 1044
BsuRI GGCC 4 cut(s) 575, 592, 607, 977
BtgI CCRYGG 2 cut(s) 511, 840
BtgZI GCGATG 1 cut(s) 712
BtrI CACGTC 1 cut(s) 559
BtsCI GGATG 5 cut(s) 394, 547, 559, 918, 978
BveI ACCTGC 1 cut(s) 202
Cac8I GCNNGC 6 cut(s) 494, 644, 648, 652, 688, 724
CfoI GCGC 2 cut(s) 688, 690
Cfr10I RCCGGY 3 cut(s) 501, 989, 1151
Cfr13I GGNCC 3 cut(s) 574, 591, 606
CseI GACGC 1 cut(s) 271
Csp6I GTAC 1 cut(s) 1162
CspAI ACCGGT 1 cut(s) 501
CviAII CATG 8 cut(s) 331, 364, 512, 568, 634, 660, 778, 841
CviQI GTAC 1 cut(s) 1162
DdeI CTNAG 5 cut(s) 221, 454, 671, 808, 1166
DpnI GATC 4 cut(s) 34, 193, 272, 336
DpnII GATC 4 cut(s) 32, 191, 270, 334
Eam1104I CTCTTC 2 cut(s) 367, 908
EarI CTCTTC 2 cut(s) 367, 908
Eco130I CCWWGG 4 cut(s) 511, 622, 840, 868
Eco31I GGTCTC 1 cut(s) 1060
Eco88I CYCGRG 1 cut(s) 1055
EcoRI GAATTC 2 cut(s) 390, 529
EcoRII CCWGG 1 cut(s) 1069
EcoT14I CCWWGG 4 cut(s) 511, 622, 840, 868
ErhI CCWWGG 4 cut(s) 511, 622, 840, 868
FaeI CATG 8 cut(s) 334, 367, 515, 571, 637, 663, 781, 844
FaqI GGGAC 2 cut(s) 176, 1190
FatI CATG 8 cut(s) 330, 363, 511, 567, 633, 659, 777, 840
FblI GTMKAC 2 cut(s) 765, 1156
Fnu4HI GCNGC 4 cut(s) 655, 732, 1128, 1131
FokI GGATG 5 cut(s) 381, 554, 566, 925, 965
Fsp4HI GCNGC 4 cut(s) 655, 732, 1128, 1131
FspBI CTAG 4 cut(s) 623, 647, 827, 1025
GlaI GCGC 2 cut(s) 687, 689
GluI GCNGC 4 cut(s) 655, 732, 1128, 1131
GsaI CCCAGC 1 cut(s) 1092
GsuI CTGGAG 1 cut(s) 306
HaeIII GGCC 4 cut(s) 575, 592, 607, 977
HapII CCGG 3 cut(s) 502, 990, 1152
HgaI GACGC 1 cut(s) 271
HhaI GCGC 2 cut(s) 688, 690
Hin1II CATG 8 cut(s) 334, 367, 515, 571, 637, 663, 781, 844
Hin6I GCGC 2 cut(s) 686, 688
HinP1I GCGC 2 cut(s) 686, 688
HinfI GANTC 4 cut(s) 11, 102, 247, 964
HpaII CCGG 3 cut(s) 502, 990, 1152
HphI GGTGA 2 cut(s) 826, 1091
Hpy166II GTNNAC 4 cut(s) 67, 706, 766, 1157
Hpy188I TCNGA 3 cut(s) 25, 196, 361
Hpy188III TCNNGA 8 cut(s) 106, 259, 323, 398, 539, 742, 961, 982
Hpy8I GTNNAC 4 cut(s) 67, 706, 766, 1157
HpyAV CCTTC 6 cut(s) 85, 429, 619, 784, 799, 1177
HpyCH4III ACNGT 5 cut(s) 56, 405, 1097, 1148, 1161
HpyCH4IV ACGT 2 cut(s) 352, 558
HpyCH4V TGCA 7 cut(s) 349, 385, 706, 722, 734, 783, 798
HpyF10VI GCNNNNNNNGC 4 cut(s) 317, 430, 660, 728
HpyF3I CTNAG 5 cut(s) 221, 454, 671, 808, 1166
HpySE526I ACGT 2 cut(s) 352, 558
Hsp92II CATG 8 cut(s) 334, 367, 515, 571, 637, 663, 781, 844
HspAI GCGC 2 cut(s) 686, 688
Kzo9I GATC 4 cut(s) 32, 191, 270, 334
LguI GCTCTTC 1 cut(s) 367
LmnI GCTCC 3 cut(s) 325, 629, 872
Lsp1109I GCAGC 4 cut(s) 666, 718, 1139, 1142
LweI GCATC 2 cut(s) 234, 735
MaeI CTAG 4 cut(s) 623, 647, 827, 1025
MaeII ACGT 2 cut(s) 352, 558
MaeIII GTNAC 4 cut(s) 547, 713, 742, 1097
MalI GATC 4 cut(s) 34, 193, 272, 336
MbiI CCGCTC 1 cut(s) 1060
MboI GATC 4 cut(s) 32, 191, 270, 334
MboII GAAGA 7 cut(s) 71, 121, 220, 271, 333, 384, 895
MfeI CAATTG 1 cut(s) 487
MflI RGATCY 1 cut(s) 334
MhlI GDGCHC 1 cut(s) 708
MluCI AATT 5 cut(s) 47, 390, 409, 487, 529
MluI ACGCGT 1 cut(s) 74
MlyI GAGTC 3 cut(s) 5, 96, 241
MseI TTAA 4 cut(s) 117, 158, 252, 1004
MslI CAYNNNNRTG 3 cut(s) 711, 839, 1146
MspI CCGG 3 cut(s) 502, 990, 1152
MspR9I CCNGG 1 cut(s) 1071
MunI CAATTG 1 cut(s) 487
MvaI CCWGG 1 cut(s) 1071
MvnI CGCG 2 cut(s) 76, 688
MwoI GCNNNNNNNGC 4 cut(s) 317, 430, 660, 728
NcoI CCATGG 2 cut(s) 511, 840
NdeII GATC 4 cut(s) 32, 191, 270, 334
NheI GCTAGC 1 cut(s) 646
NlaIII CATG 8 cut(s) 334, 367, 515, 571, 637, 663, 781, 844
NlaIV GGNNCC 3 cut(s) 576, 631, 874
NmuCI GTSAC 3 cut(s) 713, 742, 1097
NspI RCATGY 1 cut(s) 663
OliI CACNNNNGTG 2 cut(s) 711, 1146
PaqCI CACCTGC 1 cut(s) 202
PauI GCGCGC 1 cut(s) 686
PciSI GCTCTTC 1 cut(s) 367
PcsI WCGNNNNNNNCGW 1 cut(s) 889
PfeI GAWTC 1 cut(s) 964
PflMI CCANNNNNTGG 1 cut(s) 1117
PinAI ACCGGT 1 cut(s) 501
PkrI GCNGC 4 cut(s) 656, 733, 1129, 1132
Ple19I CGATCG 1 cut(s) 194
PleI GAGTC 3 cut(s) 5, 96, 241
PpsI GAGTC 3 cut(s) 5, 96, 241
Psp1406I AACGTT 1 cut(s) 352
Psp6I CCWGG 1 cut(s) 1069
PspFI CCCAGC 1 cut(s) 1088
PspGI CCWGG 1 cut(s) 1069
PspN4I GGNNCC 3 cut(s) 576, 631, 874
PspPI GGNCC 3 cut(s) 574, 591, 606
PsuI RGATCY 1 cut(s) 334
PteI GCGCGC 1 cut(s) 686
PvuI CGATCG 1 cut(s) 194
RsaI GTAC 1 cut(s) 1163
RsaNI GTAC 1 cut(s) 1162
RseI CAYNNNNRTG 3 cut(s) 711, 839, 1146
SapI GCTCTTC 1 cut(s) 367
SaqAI TTAA 4 cut(s) 117, 158, 252, 1004
SatI GCNGC 4 cut(s) 655, 732, 1128, 1131
Sau3AI GATC 4 cut(s) 32, 191, 270, 334
Sau96I GGNCC 3 cut(s) 574, 591, 606
SchI GAGTC 3 cut(s) 5, 96, 241
ScrFI CCNGG 1 cut(s) 1071
SduI GDGCHC 1 cut(s) 708
SfaNI GCATC 2 cut(s) 234, 735
SmiMI CAYNNNNRTG 3 cut(s) 711, 839, 1146
Sse9I AATT 5 cut(s) 47, 390, 409, 487, 529
SsiI CCGC 1 cut(s) 1060
SspMI CTAG 4 cut(s) 623, 647, 827, 1025
StyD4I CCNGG 1 cut(s) 1069
StyI CCWWGG 4 cut(s) 511, 622, 840, 868
TaaI ACNGT 5 cut(s) 56, 405, 1097, 1148, 1161
TaiI ACGT 2 cut(s) 355, 561
TasI AATT 5 cut(s) 47, 390, 409, 487, 529
TfiI GAWTC 1 cut(s) 964
Tru1I TTAA 4 cut(s) 117, 158, 252, 1004
Tru9I TTAA 4 cut(s) 117, 158, 252, 1004
TseFI GTSAC 3 cut(s) 713, 742, 1097
TseI GCWGC 4 cut(s) 654, 731, 1127, 1130
Tsp45I GTSAC 3 cut(s) 713, 742, 1097
TspDTI ATGAA 8 cut(s) 132, 163, 227, 383, 578, 766, 805, 932
TspGWI ACGGA 1 cut(s) 566
Van91I CCANNNNNTGG 1 cut(s) 1117
VneI GTGCAC 1 cut(s) 704
XapI RAATTY 2 cut(s) 390, 529
XceI RCATGY 1 cut(s) 663
XmaJI CCTAGG 1 cut(s) 622
XmiI GTMKAC 2 cut(s) 765, 1156
XspI CTAG 4 cut(s) 623, 647, 827, 1025
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.