pycom06g04030

Haemolysin-III related

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0001650
Physical Location & Seq
Forward (+)
5081238 .. 5082343
1106 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g04030.1

Sequence Viewer

Length: 846 bp
ATGATTAGAAGAACCGATTTGCACAAAATTCATCCAGAATTGTTGAATTGCCTCCCTTCACTAGCTTTTCCATCTATGGATTTTCTCTCCTCAGTTTCAGGGAATATAAGGGAACTTCTCGCCAATTGCTTGCCTGACCGGTTCTCCCACACCAATCAAACAGAGAATTCTGTTCTGCATGGAGTAACAGATGACGTGATGAACATGGTGGCCCCCCTTATGAATCGATCCATAACAAGGTGGCCCTTCTTTGCATTCCTAGGTGGAGCCATGTTCTGCTTGCTAGCCAGCAGTACATGCCATCTCCTTGCTTGCCATTCGGCGCGCCTTTCCTATATAGTGCTCAGATGTGACTATGCAGGCATCGCTGCACTCATCACAACCTCATTTTACCCTCCTGTCTACTACTCCTTCATGTGCAACCCCTTCTTTCGTAACCTCTACTTAAGTTTCATAACAGTGCTAGGTATCACCACCATTATTTTCTCCCTCCTCCCATTTTTTCAAGGACCCAAGTTTCGTGTCTATCGTGCTTCTCTCTTCTTTGTGATGGGTGTGTCAGGTGTCGTGCCTTTGGTTCATAAGCTCATAGTGTTCAGGAATCAGCCAGAGGCCATCCAGACTACCGGCTATGAGGTGTTAATGGGGGTTCTTTATGGACTAGGGGCATTGATTTATGCTACAAGGGTACCGGAGCGGTGGAGACCGGGGAAGTTTGATATTGCTGGCCACAGCCACCAGCTTTTCCATATTTTGGTTGTGGCAGCAGCTTACACTCACTACCGAGCTGGTCTACTGTACCTAAGATGGAGGGACTTGAAGGGTTGTCAGCTTGTATTGAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04152 ko04211 ko04920 ko04932 map04152 map04211 map04920 map04932
Pfam Domains
Protein Families
HlyIII

Protein Analysis

282

Amino Acids

31.84

Weight (kDa)

9.36

Isoelectric Point (pI)

45.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HlyIII PF03006 76 - 261 1.8e-56 Haemolysin-III related
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 688
AccB1I GGYRCC 1 cut(s) 688
AccB7I CCANNNNNTGG 1 cut(s) 754
AccBSI CCGCTC 1 cut(s) 697
AccI GTMKAC 2 cut(s) 402, 793
AccII CGCG 1 cut(s) 325
AciI CCGC 1 cut(s) 697
AclWI GGATC 1 cut(s) 222
AcoI YGGCCR 1 cut(s) 727
AcsI RAATTY 2 cut(s) 27, 166
AfaI GTAC 3 cut(s) 295, 690, 800
AfiI CCNNNNNNNGG 2 cut(s) 237, 754
AflII CTTAAG 1 cut(s) 445
AgeI ACCGGT 1 cut(s) 138
AgsI TTSAA 3 cut(s) 46, 506, 820
AjiI CACGTC 1 cut(s) 196
AluBI AGCT 6 cut(s) 65, 586, 742, 770, 788, 832
AluI AGCT 6 cut(s) 65, 586, 742, 770, 788, 832
Alw21I GWGCWC 1 cut(s) 345
Alw26I GTCTC 1 cut(s) 697
AlwI GGATC 1 cut(s) 222
AoxI GGCC 4 cut(s) 210, 242, 612, 727
ApeKI GCWGC 3 cut(s) 368, 764, 767
ApoI RAATTY 2 cut(s) 27, 166
ArsI GACNNNNNNTTYG 2 cut(s) 501, 533
AscI GGCGCGCC 1 cut(s) 323
AsiGI ACCGGT 1 cut(s) 138
Asp718I GGTACC 1 cut(s) 688
AspA2I CCTAGG 1 cut(s) 259
AspLEI GCGC 2 cut(s) 325, 327
AspS9I GGNCC 3 cut(s) 211, 243, 509
AsuC2I CCSGG 1 cut(s) 708
AsuHPI GGTGA 1 cut(s) 463
AsuNHI GCTAGC 1 cut(s) 283
AvaII GGWCC 1 cut(s) 509
AvrII CCTAGG 1 cut(s) 259
BaeI ACNNNNGTAYC 2 cut(s) 672, 705
BalI TGGCCA 1 cut(s) 729
BanI GGYRCC 1 cut(s) 688
Bbv12I GWGCWC 1 cut(s) 345
BbvI GCAGC 3 cut(s) 355, 776, 779
BccI CCATC 5 cut(s) 79, 309, 544, 623, 801
BcnI CCSGG 1 cut(s) 708
BcoDI GTCTC 1 cut(s) 697
BfaI CTAG 5 cut(s) 62, 260, 284, 464, 662
BfrI CTTAAG 1 cut(s) 445
BisI GCNGC 3 cut(s) 369, 765, 768
BlnI CCTAGG 1 cut(s) 259
BlsI GCNGC 3 cut(s) 370, 766, 769
Bme1390I CCNGG 1 cut(s) 708
Bme18I GGWCC 1 cut(s) 509
BmgBI CACGTC 1 cut(s) 196
BmgT120I GGNCC 3 cut(s) 211, 243, 509
BmiI GGNNCC 4 cut(s) 213, 268, 511, 690
BmrFI CCNGG 1 cut(s) 708
BmsI GCATC 1 cut(s) 372
BmtI GCTAGC 1 cut(s) 287
BpuMI CCSGG 1 cut(s) 708
Bsa29I ATCGAT 1 cut(s) 226
BsaI GGTCTC 1 cut(s) 697
BsaJI CCNNGG 2 cut(s) 259, 707
BsaWI WCCGGW 2 cut(s) 138, 691
BsaXI ACNNNNNCTCC 2 cut(s) 128, 158
Bsc4I CCNNNNNNNGG 2 cut(s) 237, 754
Bse118I RCCGGY 2 cut(s) 138, 626
BseCI ATCGAT 1 cut(s) 226
BseDI CCNNGG 2 cut(s) 259, 707
BseGI GGATG 2 cut(s) 31, 615
BseLI CCNNNNNNNGG 2 cut(s) 237, 754
BseMII CTCAG 2 cut(s) 105, 358
BsePI GCGCGC 1 cut(s) 323
BseRI GAGGAG 2 cut(s) 79, 482
BseXI GCAGC 3 cut(s) 355, 776, 779
BsgI GTGCAG 1 cut(s) 354
Bsh1236I CGCG 1 cut(s) 325
BshFI GGCC 4 cut(s) 212, 244, 614, 729
BshNI GGYRCC 1 cut(s) 688
BshTI ACCGGT 1 cut(s) 138
BshVI ATCGAT 1 cut(s) 226
BsiHKAI GWGCWC 1 cut(s) 345
BsiSI CCGG 4 cut(s) 139, 627, 692, 707
BslFI GGGAC 1 cut(s) 827
BslI CCNNNNNNNGG 2 cut(s) 237, 754
BsmAI GTCTC 1 cut(s) 697
BsmFI GGGAC 1 cut(s) 827
BsmI GAATGC 1 cut(s) 254
BsnI GGCC 4 cut(s) 212, 244, 614, 729
Bso31I GGTCTC 1 cut(s) 697
Bsp1286I GDGCHC 1 cut(s) 345
Bsp143I GATC 1 cut(s) 227
BspACI CCGC 1 cut(s) 697
BspANI GGCC 4 cut(s) 212, 244, 614, 729
BspCNI CTCAG 2 cut(s) 104, 357
BspDI ATCGAT 1 cut(s) 226
BspFNI CGCG 1 cut(s) 325
BspLI GGNNCC 4 cut(s) 213, 268, 511, 690
BspOI GCTAGC 1 cut(s) 287
BspPI GGATC 1 cut(s) 222
BspT107I GGYRCC 1 cut(s) 688
BspTI CTTAAG 1 cut(s) 445
BspTNI GGTCTC 1 cut(s) 697
BsrBI CCGCTC 1 cut(s) 697
BsrFI RCCGGY 2 cut(s) 138, 626
BssAI RCCGGY 2 cut(s) 138, 626
BssECI CCNNGG 2 cut(s) 259, 707
BssHII GCGCGC 1 cut(s) 323
BssMI GATC 1 cut(s) 227
BssT1I CCWWGG 1 cut(s) 259
Bst4CI ACNGT 2 cut(s) 460, 798
Bst6I CTCTTC 1 cut(s) 545
BstAFI CTTAAG 1 cut(s) 445
BstAPI GCANNNNNTGC 1 cut(s) 297
BstC8I GCNNGC 8 cut(s) 131, 281, 285, 289, 313, 325, 361, 727
BstDEI CTNAG 3 cut(s) 91, 344, 803
BstF5I GGATG 2 cut(s) 31, 615
BstFNI CGCG 1 cut(s) 325
BstHHI GCGC 2 cut(s) 325, 327
BstKTI GATC 1 cut(s) 230
BstMAI GTCTC 1 cut(s) 697
BstMBI GATC 1 cut(s) 227
BstMWI GCNNNNNNNGC 2 cut(s) 297, 365
BstNSI RCATGY 1 cut(s) 300
BstSCI CCNGG 1 cut(s) 706
BstUI CGCG 1 cut(s) 325
BstV1I GCAGC 3 cut(s) 355, 776, 779
Bsu15I ATCGAT 1 cut(s) 226
BsuRI GGCC 4 cut(s) 212, 244, 614, 729
BsuTUI ATCGAT 1 cut(s) 226
BtgZI GCGATG 1 cut(s) 349
BtrI CACGTC 1 cut(s) 196
BtsCI GGATG 2 cut(s) 31, 615
BtsIMutI CAGTG 1 cut(s) 465
Cac8I GCNNGC 8 cut(s) 131, 281, 285, 289, 313, 325, 361, 727
CfoI GCGC 2 cut(s) 325, 327
Cfr10I RCCGGY 2 cut(s) 138, 626
Cfr13I GGNCC 3 cut(s) 211, 243, 509
ClaI ATCGAT 1 cut(s) 226
Csp6I GTAC 3 cut(s) 294, 689, 799
CspAI ACCGGT 1 cut(s) 138
CviAII CATG 5 cut(s) 179, 205, 271, 297, 415
CviQI GTAC 3 cut(s) 294, 689, 799
DdeI CTNAG 3 cut(s) 91, 344, 803
DpnI GATC 1 cut(s) 229
DpnII GATC 1 cut(s) 227
EaeI YGGCCR 1 cut(s) 727
Eam1104I CTCTTC 1 cut(s) 545
EarI CTCTTC 1 cut(s) 545
Eco130I CCWWGG 1 cut(s) 259
Eco31I GGTCTC 1 cut(s) 697
Eco47I GGWCC 1 cut(s) 509
EcoO109I RGGNCCY 1 cut(s) 509
EcoRI GAATTC 1 cut(s) 166
EcoT14I CCWWGG 1 cut(s) 259
ErhI CCWWGG 1 cut(s) 259
FaeI CATG 5 cut(s) 182, 208, 274, 300, 418
FaqI GGGAC 1 cut(s) 827
FatI CATG 5 cut(s) 178, 204, 270, 296, 414
FblI GTMKAC 2 cut(s) 402, 793
Fnu4HI GCNGC 3 cut(s) 369, 765, 768
FokI GGATG 2 cut(s) 18, 602
Fsp4HI GCNGC 3 cut(s) 369, 765, 768
FspBI CTAG 5 cut(s) 62, 260, 284, 464, 662
GlaI GCGC 2 cut(s) 324, 326
GluI GCNGC 3 cut(s) 369, 765, 768
HaeIII GGCC 4 cut(s) 212, 244, 614, 729
HapII CCGG 4 cut(s) 139, 627, 692, 707
HhaI GCGC 2 cut(s) 325, 327
Hin1II CATG 5 cut(s) 182, 208, 274, 300, 418
Hin6I GCGC 2 cut(s) 323, 325
HinP1I GCGC 2 cut(s) 323, 325
HinfI GANTC 2 cut(s) 223, 601
HpaII CCGG 4 cut(s) 139, 627, 692, 707
HphI GGTGA 1 cut(s) 463
Hpy166II GTNNAC 2 cut(s) 403, 794
Hpy188I TCNGA 1 cut(s) 347
Hpy188III TCNNGA 3 cut(s) 35, 598, 619
Hpy8I GTNNAC 2 cut(s) 403, 794
HpyAV CCTTC 5 cut(s) 66, 256, 421, 436, 814
HpyCH4III ACNGT 2 cut(s) 460, 798
HpyCH4IV ACGT 1 cut(s) 195
HpyCH4V TGCA 6 cut(s) 22, 178, 254, 359, 371, 420
HpyF10VI GCNNNNNNNGC 2 cut(s) 297, 365
HpyF3I CTNAG 3 cut(s) 91, 344, 803
HpySE526I ACGT 1 cut(s) 195
Hsp92II CATG 5 cut(s) 182, 208, 274, 300, 418
HspAI GCGC 2 cut(s) 323, 325
KpnI GGTACC 1 cut(s) 692
Kzo9I GATC 1 cut(s) 227
LmnI GCTCC 2 cut(s) 266, 694
Lsp1109I GCAGC 3 cut(s) 355, 776, 779
LweI GCATC 1 cut(s) 372
MaeI CTAG 5 cut(s) 62, 260, 284, 464, 662
MaeII ACGT 1 cut(s) 195
MaeIII GTNAC 3 cut(s) 184, 350, 434
MalI GATC 1 cut(s) 229
MbiI CCGCTC 1 cut(s) 697
MboI GATC 1 cut(s) 227
MboII GAAGA 2 cut(s) 21, 532
MfeI CAATTG 1 cut(s) 124
MhlI GDGCHC 1 cut(s) 345
MlsI TGGCCA 1 cut(s) 729
MluCI AATT 5 cut(s) 27, 38, 46, 124, 166
MluNI TGGCCA 1 cut(s) 729
Mox20I TGGCCA 1 cut(s) 729
MscI TGGCCA 1 cut(s) 729
MseI TTAA 2 cut(s) 446, 641
MslI CAYNNNNRTG 1 cut(s) 458
Msp20I TGGCCA 1 cut(s) 729
MspCI CTTAAG 1 cut(s) 445
MspI CCGG 4 cut(s) 139, 627, 692, 707
MspR9I CCNGG 1 cut(s) 708
MunI CAATTG 1 cut(s) 124
Mva1269I GAATGC 1 cut(s) 254
MvnI CGCG 1 cut(s) 325
MwoI GCNNNNNNNGC 2 cut(s) 297, 365
NciI CCSGG 1 cut(s) 708
NdeII GATC 1 cut(s) 227
NheI GCTAGC 1 cut(s) 283
NlaIII CATG 5 cut(s) 182, 208, 274, 300, 418
NlaIV GGNNCC 4 cut(s) 213, 268, 511, 690
NmuCI GTSAC 1 cut(s) 350
NspI RCATGY 1 cut(s) 300
PalAI GGCGCGCC 1 cut(s) 323
PauI GCGCGC 1 cut(s) 323
PcsI WCGNNNNNNNCGW 1 cut(s) 526
PctI GAATGC 1 cut(s) 254
PfeI GAWTC 2 cut(s) 223, 601
PflMI CCANNNNNTGG 1 cut(s) 754
PinAI ACCGGT 1 cut(s) 138
PkrI GCNGC 3 cut(s) 370, 766, 769
PpuMI RGGWCCY 1 cut(s) 509
Psp5II RGGWCCY 1 cut(s) 509
PspN4I GGNNCC 4 cut(s) 213, 268, 511, 690
PspPI GGNCC 3 cut(s) 211, 243, 509
PspPPI RGGWCCY 1 cut(s) 509
PteI GCGCGC 1 cut(s) 323
RsaI GTAC 3 cut(s) 295, 690, 800
RsaNI GTAC 3 cut(s) 294, 689, 799
RseI CAYNNNNRTG 1 cut(s) 458
SaqAI TTAA 2 cut(s) 446, 641
SatI GCNGC 3 cut(s) 369, 765, 768
Sau3AI GATC 1 cut(s) 227
Sau96I GGNCC 3 cut(s) 211, 243, 509
ScrFI CCNGG 1 cut(s) 708
SduI GDGCHC 1 cut(s) 345
SfaNI GCATC 1 cut(s) 372
SgsI GGCGCGCC 1 cut(s) 323
SinI GGWCC 1 cut(s) 509
SmiMI CAYNNNNRTG 1 cut(s) 458
SmlI CTYRAG 1 cut(s) 445
SmoI CTYRAG 1 cut(s) 445
Sse9I AATT 5 cut(s) 27, 38, 46, 124, 166
SsiI CCGC 1 cut(s) 697
SspMI CTAG 5 cut(s) 62, 260, 284, 464, 662
StyD4I CCNGG 1 cut(s) 706
StyI CCWWGG 1 cut(s) 259
TaaI ACNGT 2 cut(s) 460, 798
TaiI ACGT 1 cut(s) 198
TaqI TCGA 1 cut(s) 226
TasI AATT 5 cut(s) 27, 38, 46, 124, 166
TatI WGTACW 1 cut(s) 293
TfiI GAWTC 2 cut(s) 223, 601
Tru1I TTAA 2 cut(s) 446, 641
Tru9I TTAA 2 cut(s) 446, 641
TscAI CASTG 1 cut(s) 465
TseFI GTSAC 1 cut(s) 350
TseI GCWGC 3 cut(s) 368, 764, 767
Tsp45I GTSAC 1 cut(s) 350
TspDTI ATGAA 6 cut(s) 20, 215, 236, 403, 442, 569
TspRI CASTG 1 cut(s) 465
Van91I CCANNNNNTGG 1 cut(s) 754
Vha464I CTTAAG 1 cut(s) 445
VpaK11BI GGWCC 1 cut(s) 509
XapI RAATTY 2 cut(s) 27, 166
XceI RCATGY 1 cut(s) 300
XmaJI CCTAGG 1 cut(s) 259
XmiI GTMKAC 2 cut(s) 402, 793
XspI CTAG 5 cut(s) 62, 260, 284, 464, 662
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.