Prupe.5G057500_v2.0.a1

Haemolysin-III related

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0001650
Physical Location & Seq
Forward (+)
6243447 .. 6246638
3192 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G057500.2

Sequence Viewer

Length: 1377 bp
ATGTTCCCTTGTATAACTCTGTTTCTCGAAGAAACGTTTTTGTCCTTCAATGCCTATTGTATTAGCTTATGGTGTCTTTCCTCAAATTTTCATGAGACATTAGTTTGTAGTCTTGTTTTTAGTATGAGGATGCTCCTCAATGCTTCTTGTTTCTGGGTTGTAGAGATGAGAGCATTAAATAATCAATTTGACTGTGAAAATGGAAACAAAGTGTCTTCAAAGGAAGGGAAGGGAACGAGGCTATGGAAGAAAGTGAAATATCAGCTTATTGAATACCATGCATTGCCTAGCTTTTTAAGGGACAACGAGTTCATTCTGGGTTATTACCGATCAGAATGGCCATTGAAGCAGGTGTTTCTAAGCATCTTCTCCATTCATAATGAGACTCTCAATGTGTGGACGCATTTGATCGGGTTCTTCCTTTTCCTTTTCCTAACCATATACACAGCAACAAAAGCTCCAGACATCATGGATCTTTCTTCCTTCCAACGTTTGCCTGACATGATTAGAAAAGCTGAGTTTTACAAAATTCATCCAGAACTGTTGAATTGCCTCCCTTCACTACCCAACCTGTCTGATATTTATAGATTAAAAGACGAATTGAAGCCATCTTTGCCCTCTATGGATTTCATCTCCTCAGTTTCAGGGAATATAAGGGAACTTCTCGCCAAATGCTTGCCTGAGCGGTTCTCACATGCCTATCAAACAGAGAATTCTGTTCTGCATGGAGTTACAGATGATGTGATGAACATGGTGGCCCCCCTTATGTATCGACCTATTACAAGGTGGCCCTTCTTTGTCTTCCTAGGTGGAGCCATGTTCTGCTTGCTAGCCAGCAGCACATGCCACCTCCTTGCTTGCCATTCAGCGCGCCTTTCCTACATTGTGCTTAGATGTGACTATGCAGGCATCGCTGCACTCATCACAACCTCATTTTACCCTCCTGTTTACTACTCCTTCATGTGCAACCCCTTCTTTTGCAACCTCTACTTGGGCTTCATAACAATACTAGGTATCGCCACCATTGTTTTCTCCCTCCTCCCACTTTTTCAAGGACCCAAGTTCCGTAGTTTTCGTGCTTCTCTCTATTTTGGGATGGGCGTGTCTGGTGTCGTTCCTTTAGTTCATAAACTCATAGTGTTCAGGAATCAACCAGAAGCCATTCAGACAACCGGCCTTGAAGTGTTAATGGGGGTTCTTTATGGACTGGGGGCTTTGATTTATGCCACAAGGATACCGGAGCGGTGGAGACCAGGGAAGTTTGATATTGCTGGCCAGAGTCACCAGCTTTTCCATATCTTGGTTGTGGCGGCAGCTTACACACACTATCGTGCTGGGCTGGTGTACCTAAGATGGAGGGACTTAGAGGGTTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04152 ko04211 ko04920 ko04932 map04152 map04211 map04920 map04932
Pfam Domains
Protein Families
HlyIII

Protein Analysis

459

Amino Acids

52.55

Weight (kDa)

8.53

Isoelectric Point (pI)

41.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 340
Acc36I ACCTGC 1 cut(s) 340
AccB7I CCANNNNNTGG 1 cut(s) 1300
AccBSI CCGCTC 2 cut(s) 685, 1243
AccII CGCG 1 cut(s) 871
AciI CCGC 3 cut(s) 685, 1243, 1310
AclI AACGTT 2 cut(s) 35, 490
AclWI GGATC 1 cut(s) 480
AcoI YGGCCR 2 cut(s) 338, 1273
AcsI RAATTY 3 cut(s) 85, 528, 712
AfaI GTAC 1 cut(s) 1346
AfiI CCNNNNNNNGG 2 cut(s) 991, 1300
AgsI TTSAA 8 cut(s) 49, 219, 272, 346, 547, 604, 1052, 1181
AjnI CCWGG 1 cut(s) 1252
AleI CACNNNNGTG 1 cut(s) 1329
AloI GAACNNNNNNTCC 2 cut(s) 293, 325
AluBI AGCT 7 cut(s) 66, 265, 291, 458, 515, 1288, 1316
AluI AGCT 7 cut(s) 66, 265, 291, 458, 515, 1288, 1316
Alw26I GTCTC 3 cut(s) 89, 377, 1243
AlwI GGATC 1 cut(s) 480
AoxI GGCC 5 cut(s) 338, 756, 788, 1174, 1273
ApeKI GCWGC 3 cut(s) 837, 914, 1313
ApoI RAATTY 3 cut(s) 85, 528, 712
Asp700I GAANNNNTTC 1 cut(s) 1161
AspA2I CCTAGG 1 cut(s) 805
AspLEI GCGC 2 cut(s) 871, 873
AspS9I GGNCC 3 cut(s) 757, 789, 1055
AsuHPI GGTGA 1 cut(s) 1274
AsuNHI GCTAGC 1 cut(s) 829
AvaII GGWCC 1 cut(s) 1055
AvrII CCTAGG 1 cut(s) 805
BalI TGGCCA 2 cut(s) 340, 1275
BbsI GAAGAC 2 cut(s) 207, 793
BbvI GCAGC 3 cut(s) 849, 901, 1325
BccI CCATC 3 cut(s) 616, 1090, 1347
BciT130I CCWGG 1 cut(s) 1254
BciVI GTATCC 1 cut(s) 1227
BcoDI GTCTC 3 cut(s) 89, 377, 1243
BfaI CTAG 4 cut(s) 288, 806, 830, 1010
BfuAI ACCTGC 1 cut(s) 340
BfuI GTATCC 1 cut(s) 1227
BisI GCNGC 4 cut(s) 838, 915, 1311, 1314
BlnI CCTAGG 1 cut(s) 805
BlsI GCNGC 4 cut(s) 839, 916, 1312, 1315
Bme1390I CCNGG 1 cut(s) 1254
Bme18I GGWCC 1 cut(s) 1055
BmgT120I GGNCC 3 cut(s) 757, 789, 1055
BmiI GGNNCC 3 cut(s) 759, 814, 1057
BmrFI CCNGG 1 cut(s) 1254
BmrI ACTGGG 1 cut(s) 1217
BmsI GCATC 3 cut(s) 120, 372, 918
BmtI GCTAGC 1 cut(s) 833
BmuI ACTGGG 1 cut(s) 1217
BpiI GAAGAC 2 cut(s) 207, 793
BplI GAGNNNNNCTC 2 cut(s) 674, 706
BpmI CTGGAG 1 cut(s) 444
Bpu10I CCTNAGC 1 cut(s) 681
BsaI GGTCTC 1 cut(s) 1243
BsaJI CCNNGG 2 cut(s) 805, 1253
BsaWI WCCGGW 1 cut(s) 1237
BsaXI ACNNNNNCTCC 2 cut(s) 442, 472
Bsc4I CCNNNNNNNGG 2 cut(s) 991, 1300
Bse118I RCCGGY 1 cut(s) 1172
Bse1I ACTGG 1 cut(s) 1212
Bse3DI GCAATG 1 cut(s) 281
BseBI CCWGG 1 cut(s) 1254
BseDI CCNNGG 2 cut(s) 805, 1253
BseGI GGATG 3 cut(s) 135, 532, 1101
BseLI CCNNNNNNNGG 2 cut(s) 991, 1300
BseMI GCAATG 1 cut(s) 281
BseMII CTCAG 3 cut(s) 507, 651, 672
BseNI ACTGG 1 cut(s) 1212
BsePI GCGCGC 1 cut(s) 869
BseRI GAGGAG 3 cut(s) 125, 625, 1028
BseXI GCAGC 3 cut(s) 849, 901, 1325
BseYI CCCAGC 1 cut(s) 1334
BsgI GTGCAG 1 cut(s) 900
Bsh1236I CGCG 1 cut(s) 871
BshFI GGCC 5 cut(s) 340, 758, 790, 1176, 1275
BsiSI CCGG 2 cut(s) 1173, 1238
BslFI GGGAC 2 cut(s) 314, 1373
BslI CCNNNNNNNGG 2 cut(s) 991, 1300
BsmAI GTCTC 3 cut(s) 89, 377, 1243
BsmFI GGGAC 2 cut(s) 314, 1373
BsnI GGCC 5 cut(s) 340, 758, 790, 1176, 1275
Bso31I GGTCTC 1 cut(s) 1243
Bsp143I GATC 3 cut(s) 329, 408, 472
BspACI CCGC 3 cut(s) 685, 1243, 1310
BspANI GGCC 5 cut(s) 340, 758, 790, 1176, 1275
BspCNI CTCAG 3 cut(s) 508, 650, 673
BspFNI CGCG 1 cut(s) 871
BspHI TCATGA 1 cut(s) 91
BspLI GGNNCC 3 cut(s) 759, 814, 1057
BspMI ACCTGC 1 cut(s) 340
BspOI GCTAGC 1 cut(s) 833
BspPI GGATC 1 cut(s) 480
BspTNI GGTCTC 1 cut(s) 1243
BsrBI CCGCTC 2 cut(s) 685, 1243
BsrDI GCAATG 1 cut(s) 281
BsrFI RCCGGY 1 cut(s) 1172
BsrI ACTGG 1 cut(s) 1212
BssAI RCCGGY 1 cut(s) 1172
BssECI CCNNGG 2 cut(s) 805, 1253
BssHII GCGCGC 1 cut(s) 869
BssMI GATC 3 cut(s) 329, 408, 472
BssT1I CCWWGG 1 cut(s) 805
Bst2UI CCWGG 1 cut(s) 1254
Bst4CI ACNGT 2 cut(s) 194, 543
BstAPI GCANNNNNTGC 1 cut(s) 843
BstC8I GCNNGC 8 cut(s) 677, 827, 831, 835, 859, 871, 907, 1273
BstDEI CTNAG 7 cut(s) 359, 516, 637, 681, 890, 1349, 1363
BstF5I GGATG 3 cut(s) 135, 532, 1101
BstFNI CGCG 1 cut(s) 871
BstHHI GCGC 2 cut(s) 871, 873
BstKTI GATC 3 cut(s) 332, 411, 475
BstMAI GTCTC 3 cut(s) 89, 377, 1243
BstMBI GATC 3 cut(s) 329, 408, 472
BstMWI GCNNNNNNNGC 5 cut(s) 346, 455, 613, 843, 911
BstNI CCWGG 1 cut(s) 1254
BstNSI RCATGY 2 cut(s) 698, 846
BstSCI CCNGG 1 cut(s) 1252
BstUI CGCG 1 cut(s) 871
BstV1I GCAGC 3 cut(s) 849, 901, 1325
BstV2I GAAGAC 2 cut(s) 207, 793
BstX2I RGATCY 1 cut(s) 472
BstYI RGATCY 1 cut(s) 472
BsuI GTATCC 1 cut(s) 1227
BsuRI GGCC 5 cut(s) 340, 758, 790, 1176, 1275
BtgZI GCGATG 1 cut(s) 895
BtsCI GGATG 3 cut(s) 135, 532, 1101
BveI ACCTGC 1 cut(s) 340
Cac8I GCNNGC 8 cut(s) 677, 827, 831, 835, 859, 871, 907, 1273
CciI TCATGA 1 cut(s) 91
CfoI GCGC 2 cut(s) 871, 873
Cfr10I RCCGGY 1 cut(s) 1172
Cfr13I GGNCC 3 cut(s) 757, 789, 1055
CseI GACGC 1 cut(s) 409
Csp6I GTAC 1 cut(s) 1345
CviQI GTAC 1 cut(s) 1345
DdeI CTNAG 7 cut(s) 359, 516, 637, 681, 890, 1349, 1363
DpnI GATC 3 cut(s) 331, 410, 474
DpnII GATC 3 cut(s) 329, 408, 472
EaeI YGGCCR 2 cut(s) 338, 1273
Eco130I CCWWGG 1 cut(s) 805
Eco31I GGTCTC 1 cut(s) 1243
Eco47I GGWCC 1 cut(s) 1055
EcoO109I RGGNCCY 1 cut(s) 1055
EcoRI GAATTC 1 cut(s) 712
EcoRII CCWGG 1 cut(s) 1252
EcoT14I CCWWGG 1 cut(s) 805
EcoT22I ATGCAT 1 cut(s) 283
ErhI CCWWGG 1 cut(s) 805
FaqI GGGAC 2 cut(s) 314, 1373
Fnu4HI GCNGC 4 cut(s) 838, 915, 1311, 1314
FokI GGATG 3 cut(s) 142, 519, 1108
Fsp4HI GCNGC 4 cut(s) 838, 915, 1311, 1314
FspBI CTAG 4 cut(s) 288, 806, 830, 1010
GlaI GCGC 2 cut(s) 870, 872
GluI GCNGC 4 cut(s) 838, 915, 1311, 1314
GsaI CCCAGC 1 cut(s) 1338
GsuI CTGGAG 1 cut(s) 444
HaeIII GGCC 5 cut(s) 340, 758, 790, 1176, 1275
HapII CCGG 2 cut(s) 1173, 1238
HgaI GACGC 1 cut(s) 409
HhaI GCGC 2 cut(s) 871, 873
Hin6I GCGC 2 cut(s) 869, 871
HinP1I GCGC 2 cut(s) 869, 871
HinfI GANTC 3 cut(s) 385, 1147, 1279
HpaII CCGG 2 cut(s) 1173, 1238
HphI GGTGA 1 cut(s) 1274
Hpy166II GTNNAC 3 cut(s) 399, 949, 1345
Hpy188I TCNGA 3 cut(s) 334, 577, 1167
Hpy188III TCNNGA 5 cut(s) 26, 92, 461, 536, 1144
Hpy8I GTNNAC 3 cut(s) 399, 949, 1345
HpyAV CCTTC 8 cut(s) 55, 218, 223, 493, 567, 802, 967, 982
HpyCH4III ACNGT 2 cut(s) 194, 543
HpyCH4IV ACGT 2 cut(s) 35, 490
HpyCH4V TGCA 6 cut(s) 281, 724, 905, 917, 966, 981
HpyF10VI GCNNNNNNNGC 5 cut(s) 346, 455, 613, 843, 911
HpyF3I CTNAG 7 cut(s) 359, 516, 637, 681, 890, 1349, 1363
HpySE526I ACGT 2 cut(s) 35, 490
HspAI GCGC 2 cut(s) 869, 871
Kzo9I GATC 3 cut(s) 329, 408, 472
LmnI GCTCC 4 cut(s) 138, 463, 812, 1240
Lsp1109I GCAGC 3 cut(s) 849, 901, 1325
LweI GCATC 3 cut(s) 120, 372, 918
MaeI CTAG 4 cut(s) 288, 806, 830, 1010
MaeII ACGT 2 cut(s) 35, 490
MaeIII GTNAC 3 cut(s) 730, 896, 1280
MalI GATC 3 cut(s) 331, 410, 474
MbiI CCGCTC 2 cut(s) 685, 1243
MboI GATC 3 cut(s) 329, 408, 472
MboII GAAGA 7 cut(s) 41, 207, 259, 358, 409, 471, 793
MflI RGATCY 1 cut(s) 472
MlsI TGGCCA 2 cut(s) 340, 1275
MluCI AATT 6 cut(s) 85, 185, 528, 547, 599, 712
MluNI TGGCCA 2 cut(s) 340, 1275
MlyI GAGTC 2 cut(s) 379, 1288
MmeI TCCRAC 1 cut(s) 511
Mox20I TGGCCA 2 cut(s) 340, 1275
Mph1103I ATGCAT 1 cut(s) 283
MroXI GAANNNNTTC 1 cut(s) 1161
MscI TGGCCA 2 cut(s) 340, 1275
MseI TTAA 5 cut(s) 176, 296, 590, 1187, 1375
MslI CAYNNNNRTG 1 cut(s) 1329
Msp20I TGGCCA 2 cut(s) 340, 1275
MspI CCGG 2 cut(s) 1173, 1238
MspR9I CCNGG 1 cut(s) 1254
MvaI CCWGG 1 cut(s) 1254
MvnI CGCG 1 cut(s) 871
MwoI GCNNNNNNNGC 5 cut(s) 346, 455, 613, 843, 911
NdeII GATC 3 cut(s) 329, 408, 472
NheI GCTAGC 1 cut(s) 829
NlaIV GGNNCC 3 cut(s) 759, 814, 1057
NmuCI GTSAC 2 cut(s) 896, 1280
NsiI ATGCAT 1 cut(s) 283
NspI RCATGY 2 cut(s) 698, 846
OliI CACNNNNGTG 1 cut(s) 1329
PagI TCATGA 1 cut(s) 91
PaqCI CACCTGC 1 cut(s) 340
PauI GCGCGC 1 cut(s) 869
PdmI GAANNNNTTC 1 cut(s) 1161
PfeI GAWTC 1 cut(s) 1147
PflMI CCANNNNNTGG 1 cut(s) 1300
PkrI GCNGC 4 cut(s) 839, 916, 1312, 1315
PleI GAGTC 2 cut(s) 379, 1287
PpsI GAGTC 2 cut(s) 379, 1287
PpuMI RGGWCCY 1 cut(s) 1055
Psp1406I AACGTT 2 cut(s) 35, 490
Psp5II RGGWCCY 1 cut(s) 1055
Psp6I CCWGG 1 cut(s) 1252
PspFI CCCAGC 1 cut(s) 1334
PspGI CCWGG 1 cut(s) 1252
PspN4I GGNNCC 3 cut(s) 759, 814, 1057
PspPI GGNCC 3 cut(s) 757, 789, 1055
PspPPI RGGWCCY 1 cut(s) 1055
PsuI RGATCY 1 cut(s) 472
PteI GCGCGC 1 cut(s) 869
RsaI GTAC 1 cut(s) 1346
RsaNI GTAC 1 cut(s) 1345
RseI CAYNNNNRTG 1 cut(s) 1329
SaqAI TTAA 5 cut(s) 176, 296, 590, 1187, 1375
SatI GCNGC 4 cut(s) 838, 915, 1311, 1314
Sau3AI GATC 3 cut(s) 329, 408, 472
Sau96I GGNCC 3 cut(s) 757, 789, 1055
SchI GAGTC 2 cut(s) 379, 1288
ScrFI CCNGG 1 cut(s) 1254
SfaNI GCATC 3 cut(s) 120, 372, 918
SinI GGWCC 1 cut(s) 1055
SmiMI CAYNNNNRTG 1 cut(s) 1329
Sse9I AATT 6 cut(s) 85, 185, 528, 547, 599, 712
SsiI CCGC 3 cut(s) 685, 1243, 1310
SspMI CTAG 4 cut(s) 288, 806, 830, 1010
StyD4I CCNGG 1 cut(s) 1252
StyI CCWWGG 1 cut(s) 805
TaaI ACNGT 2 cut(s) 194, 543
TaiI ACGT 2 cut(s) 38, 493
TaqI TCGA 2 cut(s) 27, 772
TasI AATT 6 cut(s) 85, 185, 528, 547, 599, 712
TauI GCSGC 1 cut(s) 1313
TfiI GAWTC 1 cut(s) 1147
Tru1I TTAA 5 cut(s) 176, 296, 590, 1187, 1375
Tru9I TTAA 5 cut(s) 176, 296, 590, 1187, 1375
TseFI GTSAC 2 cut(s) 896, 1280
TseI GCWGC 3 cut(s) 837, 914, 1313
Tsp45I GTSAC 2 cut(s) 896, 1280
TspDTI ATGAA 9 cut(s) 80, 301, 365, 521, 619, 761, 949, 988, 1115
TspGWI ACGGA 1 cut(s) 1055
Van91I CCANNNNNTGG 1 cut(s) 1300
VpaK11BI GGWCC 1 cut(s) 1055
XapI RAATTY 3 cut(s) 85, 528, 712
XceI RCATGY 2 cut(s) 698, 846
XmaJI CCTAGG 1 cut(s) 805
XmnI GAANNNNTTC 1 cut(s) 1161
XspI CTAG 4 cut(s) 288, 806, 830, 1010
Zsp2I ATGCAT 1 cut(s) 283
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.