MD02G1242900.v1.1

4-coumarate--CoA ligase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0005541
Physical Location & Seq
Reverse (-)
29310417 .. 29312032
1616 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1242900.v1.1.491

Sequence Viewer

Length: 1566 bp
ATGTCTCTCCAAGAGTTCTCCGGCGGGGATGATCGAGTCCACATTGCCGGAAGTACTACAAATGGTTTCGATCCAAAAACCGGAATATATCACTCCCCTCTCAACCTTGGTCCCCGTTACAAAATCCCCGCCAAGCATAACCTCGACACGGCCACATTTGTTTTGTCGCAGTTTCCGCCCCATCATGAGGCCGAGTCGCGAGTTGCACTCATTGACTCGGCCACCAACGAACGAGTCACCTATGAACAGCTTCTTCATTCAATTCATGCTCTTGCTTCAGGCTTGTACCAGGCACTTGGTGTTAGAAAAGGGGATGTGGTTTTTCTCTTGTCACCTAACTCACTTTTGTACCCAACCATATGCCTGGCAGTGTTGTCAATTGGTGCAATTTTAACCACGGCAAATCCAATCAACACCGAGTCAGAAATCGGCAAGCAAGTTCGCGATTCGGGCGCCAAGCTTGCTATTTCATCTCCTGAGGAGCTTCACAAATTGGTCCCAACTGGGGTGCCTACAATATTAACATCAAGGCCGTTGAGTGGTGATGACTCACTCACCATTGAACAGTTGATTGAAGGTTATGATCCCATTCCAACCGAGTTAATGCAAGCCCGTCCGACTCAGTCAGACACTGCTGCTATTTTGTACTCCTCAGGGACTACTGGGACAAGTAAAGGTGTAGTTCTAACACATGCAAATTTCATTGCCATTATCACAATGCTCAGATGGACGGTGGACAAAACATCAGCACAGGACGATGTGTTTTTGTGCTTCATTCCTATGTTCCACATCTATGGGCTTGCTTTCTTCGGCCTTGGATTGTTTTGCGCAGGAACCACCACTGTTTTGATGCAAAGGTTTGAGTTCAACACCATGTTGGATGCTATAAAAACTCACAAAGTGAGCAACATACCAGCTGTGCCTCCGGTGATTCTTGGACTAGTAAAGTACGCAAGCAAAGCTGCCTGCGACTTGTCGTCTCTGAGGAGGGTGAGCTCAGGGGCTGCACCGTTGAGCAAAGAATTGATTGATGAGTTTAGAGAGCGGTTTCCTTGGGTTGAGATGAGGCCAGGCTATGGCCTAACGGAAAGCTGTGGTGCAGCAACTATTTTCATTTCAGACAAGCAGGCAAAGGCTCACTCCGCCTCTTGTGGGGTGCTGCTGCCAGGTTTTTCTGCTAAGATAGTGGATATCGAAACCGGGGAGGCCTTGCCGCCATATAAGGAAGGAGAGCTGTGGCTAAAGAGCCCTACTATTATGAGAGAGTATTTGGGGAATGTGGAATCAACACTTGCAACTATTGACTCAAATGGCTGGCTGAAAACTGGTGATCTTTGCTATTTTGATGAGGAAGGCTTCCTTTTCATCGTTGATCGGATAAAGGAGCTCATCAAGCATAATGGATATCAAGTTGCTCCGGCGGAATTGGAGGCCATACTTTTGAGTCATCCTCAAATTCTTGATGCAGCCATTACACCGTGTGTTGCTTACCTCCCTCTTTCTAAATGCGAACAAGCTCCTTATATCTGCATCCATATCTATATCTATATCTATCTGTGTATATAA

Protein Analysis

522

Amino Acids

56.84

Weight (kDa)

5.54

Isoelectric Point (pI)

42.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 66 - 424 1.2e-86 AMP-binding enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 829
AccB1I GGYRCC 2 cut(s) 452, 508
AccB7I CCANNNNNTGG 1 cut(s) 1076
AccBSI CCGCTC 1 cut(s) 1045
AccII CGCG 2 cut(s) 199, 444
AciI CCGC 7 cut(s) 24, 129, 176, 1045, 1143, 1214, 1421
AclWI GGATC 2 cut(s) 65, 578
AcoI YGGCCR 2 cut(s) 150, 219
AcsI RAATTY 2 cut(s) 697, 1455
AcuI CTGAAG 1 cut(s) 261
AcyI GRCGYC 1 cut(s) 453
AdeI CACNNNGTG 3 cut(s) 299, 901, 1481
AfaI GTAC 5 cut(s) 55, 287, 350, 647, 950
AfiI CCNNNNNNNGG 7 cut(s) 80, 148, 187, 505, 539, 1076, 1152
AgsI TTSAA 4 cut(s) 261, 563, 575, 868
AhdI GACNNNNNGTC 1 cut(s) 976
AhlI ACTAGT 1 cut(s) 940
AjnI CCWGG 4 cut(s) 288, 363, 1069, 1165
Alw21I GWGCWC 2 cut(s) 998, 1389
Alw26I GTCTC 2 cut(s) 9, 984
AlwI GGATC 2 cut(s) 65, 578
AlwNI CAGNNNCTG 2 cut(s) 632, 1004
AoxI GGCC 9 cut(s) 150, 189, 219, 530, 811, 1067, 1078, 1206, 1431
ApeKI GCWGC 7 cut(s) 635, 962, 1004, 1100, 1159, 1162, 1466
ApoI RAATTY 2 cut(s) 697, 1455
Asp700I GAANNNNTTC 1 cut(s) 249
AspLEI GCGC 2 cut(s) 455, 830
AspS9I GGNCC 2 cut(s) 110, 496
AsuC2I CCSGG 1 cut(s) 1201
AsuHPI GGTGA 7 cut(s) 229, 324, 547, 554, 940, 1003, 1340
AvaII GGWCC 2 cut(s) 110, 496
AxyI CCTNAGG 2 cut(s) 477, 652
BaeI ACNNNNGTAYC 2 cut(s) 332, 365
BanI GGYRCC 2 cut(s) 452, 508
BanII GRGCYC 3 cut(s) 998, 1250, 1389
Bbv12I GWGCWC 2 cut(s) 998, 1389
BbvI GCAGC 7 cut(s) 622, 949, 991, 1112, 1146, 1149, 1478
BccI CCATC 2 cut(s) 189, 720
BceAI ACGGC 3 cut(s) 165, 414, 517
BciT130I CCWGG 4 cut(s) 290, 365, 1071, 1167
BcnI CCSGG 1 cut(s) 1201
BcoDI GTCTC 2 cut(s) 9, 984
BcuI ACTAGT 1 cut(s) 940
BfaI CTAG 1 cut(s) 941
BfoI RGCGCY 1 cut(s) 456
BisI GCNGC 8 cut(s) 636, 963, 1005, 1101, 1160, 1163, 1214, 1467
BlsI GCNGC 8 cut(s) 637, 964, 1006, 1102, 1161, 1164, 1215, 1468
BmcAI AGTACT 1 cut(s) 55
Bme1390I CCNGG 5 cut(s) 290, 365, 1071, 1167, 1201
Bme18I GGWCC 2 cut(s) 110, 496
BmeRI GACNNNNNGTC 1 cut(s) 976
BmgT120I GGNCC 2 cut(s) 110, 496
BmiI GGNNCC 5 cut(s) 112, 454, 498, 510, 835
BmrFI CCNGG 5 cut(s) 290, 365, 1071, 1167, 1201
BmrI ACTGGG 2 cut(s) 513, 672
BmsI GCATC 4 cut(s) 840, 871, 1453, 1539
BmuI ACTGGG 2 cut(s) 513, 672
BplI GAGNNNNNCTC 4 cut(s) 192, 224, 1435, 1467
Bpu10I CCTNAGC 1 cut(s) 997
BpuMI CCSGG 1 cut(s) 1201
BsaHI GRCGYC 1 cut(s) 453
BsaJI CCNNGG 5 cut(s) 106, 396, 814, 1052, 1200
BsaWI WCCGGW 2 cut(s) 80, 925
Bsc4I CCNNNNNNNGG 7 cut(s) 80, 148, 187, 505, 539, 1076, 1152
Bse1I ACTGG 3 cut(s) 508, 667, 1330
Bse21I CCTNAGG 2 cut(s) 477, 652
Bse3DI GCAATG 2 cut(s) 42, 702
BseBI CCWGG 4 cut(s) 290, 365, 1071, 1167
BseDI CCNNGG 5 cut(s) 106, 396, 814, 1052, 1200
BseGI GGATG 5 cut(s) 34, 319, 886, 1447, 1530
BseLI CCNNNNNNNGG 7 cut(s) 80, 148, 187, 505, 539, 1076, 1152
BseMI GCAATG 2 cut(s) 42, 702
BseMII CTCAG 6 cut(s) 468, 635, 666, 736, 974, 1011
BseNI ACTGG 3 cut(s) 508, 667, 1330
BseRI GAGGAG 3 cut(s) 494, 640, 1000
BseXI GCAGC 7 cut(s) 622, 949, 991, 1112, 1146, 1149, 1478
BsgI GTGCAG 2 cut(s) 990, 1119
Bsh1236I CGCG 2 cut(s) 199, 444
BshFI GGCC 9 cut(s) 152, 191, 221, 532, 813, 1069, 1080, 1208, 1433
BshNI GGYRCC 2 cut(s) 452, 508
BsiHKAI GWGCWC 2 cut(s) 998, 1389
BsiSI CCGG 6 cut(s) 21, 48, 81, 926, 1200, 1418
BslFI GGGAC 4 cut(s) 96, 482, 670, 679
BslI CCNNNNNNNGG 7 cut(s) 80, 148, 187, 505, 539, 1076, 1152
BsmAI GTCTC 2 cut(s) 9, 984
BsmBI CGTCTC 1 cut(s) 984
BsmFI GGGAC 4 cut(s) 96, 482, 670, 679
BsnI GGCC 9 cut(s) 152, 191, 221, 532, 813, 1069, 1080, 1208, 1433
Bsp1286I GDGCHC 3 cut(s) 998, 1250, 1389
Bsp143I GATC 5 cut(s) 31, 70, 583, 1330, 1372
Bsp68I TCGCGA 2 cut(s) 199, 444
BspACI CCGC 7 cut(s) 24, 129, 176, 1045, 1143, 1214, 1421
BspANI GGCC 9 cut(s) 152, 191, 221, 532, 813, 1069, 1080, 1208, 1433
BspCNI CTCAG 6 cut(s) 469, 634, 665, 735, 975, 1010
BspFNI CGCG 2 cut(s) 199, 444
BspHI TCATGA 1 cut(s) 184
BspLI GGNNCC 5 cut(s) 112, 454, 498, 510, 835
BspPI GGATC 2 cut(s) 65, 578
BspT107I GGYRCC 2 cut(s) 452, 508
BsrBI CCGCTC 1 cut(s) 1045
BsrDI GCAATG 2 cut(s) 42, 702
BsrI ACTGG 3 cut(s) 508, 667, 1330
BssECI CCNNGG 5 cut(s) 106, 396, 814, 1052, 1200
BssMI GATC 5 cut(s) 31, 70, 583, 1330, 1372
BssNI GRCGYC 1 cut(s) 453
BssT1I CCWWGG 3 cut(s) 106, 814, 1052
Bst2UI CCWGG 4 cut(s) 290, 365, 1071, 1167
Bst4CI ACNGT 5 cut(s) 567, 733, 844, 1011, 1479
BstACI GRCGYC 1 cut(s) 453
BstC8I GCNNGC 8 cut(s) 434, 462, 609, 801, 955, 967, 1128, 1316
BstDEI CTNAG 7 cut(s) 477, 621, 652, 722, 983, 997, 1179
BstDSI CCRYGG 1 cut(s) 396
BstF5I GGATG 5 cut(s) 34, 319, 886, 1447, 1530
BstFNI CGCG 2 cut(s) 199, 444
BstH2I RGCGCY 1 cut(s) 456
BstHHI GCGC 2 cut(s) 455, 830
BstKTI GATC 5 cut(s) 34, 73, 586, 1333, 1375
BstMAI GTCTC 2 cut(s) 9, 984
BstMBI GATC 5 cut(s) 31, 70, 583, 1330, 1372
BstMWI GCNNNNNNNGC 6 cut(s) 175, 450, 461, 959, 1142, 1393
BstNI CCWGG 4 cut(s) 290, 365, 1071, 1167
BstNSI RCATGY 1 cut(s) 695
BstSCI CCNGG 5 cut(s) 288, 363, 1069, 1165, 1199
BstUI CGCG 2 cut(s) 199, 444
BstV1I GCAGC 7 cut(s) 622, 949, 991, 1112, 1146, 1149, 1478
BstXI CCANNNNNNTGG 3 cut(s) 296, 364, 794
Bsu36I CCTNAGG 2 cut(s) 477, 652
BsuRI GGCC 9 cut(s) 152, 191, 221, 532, 813, 1069, 1080, 1208, 1433
BtgI CCRYGG 1 cut(s) 396
BtsCI GGATG 5 cut(s) 34, 319, 886, 1447, 1530
BtsI GCAGTG 2 cut(s) 375, 630
BtsIMutI CAGTG 3 cut(s) 375, 630, 840
BtuMI TCGCGA 2 cut(s) 199, 444
Cac8I GCNNGC 8 cut(s) 434, 462, 609, 801, 955, 967, 1128, 1316
CaiI CAGNNNCTG 2 cut(s) 632, 1004
CciI TCATGA 1 cut(s) 184
CfoI GCGC 2 cut(s) 455, 830
Cfr13I GGNCC 2 cut(s) 110, 496
Csp6I GTAC 5 cut(s) 54, 286, 349, 646, 949
CviAII CATG 4 cut(s) 185, 266, 692, 874
CviQI GTAC 5 cut(s) 54, 286, 349, 646, 949
DdeI CTNAG 7 cut(s) 477, 621, 652, 722, 983, 997, 1179
DinI GGCGCC 1 cut(s) 454
DpnI GATC 5 cut(s) 33, 72, 585, 1332, 1374
DpnII GATC 5 cut(s) 31, 70, 583, 1330, 1372
DraIII CACNNNGTG 3 cut(s) 299, 901, 1481
DriI GACNNNNNGTC 1 cut(s) 976
EaeI YGGCCR 2 cut(s) 150, 219
Eam1105I GACNNNNNGTC 1 cut(s) 976
EciI GGCGGA 3 cut(s) 165, 1132, 1436
Ecl136II GAGCTC 2 cut(s) 996, 1387
Eco130I CCWWGG 3 cut(s) 106, 814, 1052
Eco147I AGGCCT 1 cut(s) 1208
Eco24I GRGCYC 3 cut(s) 998, 1250, 1389
Eco32I GATATC 2 cut(s) 1192, 1406
Eco47I GGWCC 2 cut(s) 110, 496
Eco53kI GAGCTC 2 cut(s) 996, 1387
Eco57I CTGAAG 1 cut(s) 261
Eco81I CCTNAGG 2 cut(s) 477, 652
EcoICRI GAGCTC 2 cut(s) 996, 1387
EcoRII CCWGG 4 cut(s) 288, 363, 1069, 1165
EcoRV GATATC 2 cut(s) 1192, 1406
EcoT14I CCWWGG 3 cut(s) 106, 814, 1052
EcoT38I GRGCYC 3 cut(s) 998, 1250, 1389
EgeI GGCGCC 1 cut(s) 454
EheI GGCGCC 1 cut(s) 454
ErhI CCWWGG 3 cut(s) 106, 814, 1052
Esp3I CGTCTC 1 cut(s) 984
FaeI CATG 4 cut(s) 188, 269, 695, 877
FalI AAGNNNNNCTT 2 cut(s) 1344, 1376
FaqI GGGAC 4 cut(s) 96, 482, 670, 679
FatI CATG 4 cut(s) 184, 265, 691, 873
FauI CCCGC 2 cut(s) 17, 136
FauNDI CATATG 1 cut(s) 359
Fnu4HI GCNGC 8 cut(s) 636, 963, 1005, 1101, 1160, 1163, 1214, 1467
FokI GGATG 5 cut(s) 41, 326, 893, 1434, 1517
FriOI GRGCYC 3 cut(s) 998, 1250, 1389
Fsp4HI GCNGC 8 cut(s) 636, 963, 1005, 1101, 1160, 1163, 1214, 1467
FspBI CTAG 1 cut(s) 941
FspI TGCGCA 1 cut(s) 829
GlaI GCGC 2 cut(s) 454, 829
GluI GCNGC 8 cut(s) 636, 963, 1005, 1101, 1160, 1163, 1214, 1467
HaeII RGCGCY 1 cut(s) 456
HaeIII GGCC 9 cut(s) 152, 191, 221, 532, 813, 1069, 1080, 1208, 1433
HapII CCGG 6 cut(s) 21, 48, 81, 926, 1200, 1418
HhaI GCGC 2 cut(s) 455, 830
Hin1I GRCGYC 1 cut(s) 453
Hin1II CATG 4 cut(s) 188, 269, 695, 877
Hin6I GCGC 2 cut(s) 453, 828
HinP1I GCGC 2 cut(s) 453, 828
HindIII AAGCTT 1 cut(s) 458
HpaII CCGG 6 cut(s) 21, 48, 81, 926, 1200, 1418
HphI GGTGA 7 cut(s) 229, 324, 547, 554, 940, 1003, 1340
Hpy166II GTNNAC 2 cut(s) 40, 736
Hpy188I TCNGA 7 cut(s) 424, 618, 628, 725, 984, 1120, 1377
Hpy188III TCNNGA 5 cut(s) 185, 198, 443, 476, 1460
Hpy8I GTNNAC 2 cut(s) 40, 736
HpyAV CCTTC 3 cut(s) 569, 1220, 1346
HpyCH4III ACNGT 5 cut(s) 567, 733, 844, 1011, 1479
HpyF10VI GCNNNNNNNGC 6 cut(s) 175, 450, 461, 959, 1142, 1393
HpyF3I CTNAG 7 cut(s) 477, 621, 652, 722, 983, 997, 1179
Hsp92I GRCGYC 1 cut(s) 453
Hsp92II CATG 4 cut(s) 188, 269, 695, 877
HspAI GCGC 2 cut(s) 453, 828
KasI GGCGCC 1 cut(s) 452
Kzo9I GATC 5 cut(s) 31, 70, 583, 1330, 1372
LmnI GCTCC 4 cut(s) 481, 1384, 1420, 1522
Lsp1109I GCAGC 7 cut(s) 622, 949, 991, 1112, 1146, 1149, 1478
LweI GCATC 4 cut(s) 840, 871, 1453, 1539
MaeI CTAG 1 cut(s) 941
MaeIII GTNAC 3 cut(s) 116, 235, 330
MalI GATC 5 cut(s) 33, 72, 585, 1332, 1374
MbiI CCGCTC 1 cut(s) 1045
MboI GATC 5 cut(s) 31, 70, 583, 1330, 1372
MboII GAAGA 2 cut(s) 245, 799
MfeI CAATTG 1 cut(s) 378
MhlI GDGCHC 3 cut(s) 998, 1250, 1389
MluCI AATT 8 cut(s) 261, 378, 387, 491, 697, 1022, 1424, 1455
Mly113I GGCGCC 1 cut(s) 453
MlyI GAGTC 9 cut(s) 45, 203, 209, 243, 428, 542, 613, 1298, 1453
MmeI TCCRAC 3 cut(s) 617, 641, 858
MroXI GAANNNNTTC 1 cut(s) 249
MseI TTAA 3 cut(s) 392, 521, 602
MslI CAYNNNNRTG 2 cut(s) 779, 792
MspA1I CMGCKG 1 cut(s) 917
MspI CCGG 6 cut(s) 21, 48, 81, 926, 1200, 1418
MspR9I CCNGG 5 cut(s) 290, 365, 1071, 1167, 1201
MunI CAATTG 1 cut(s) 378
MvaI CCWGG 4 cut(s) 290, 365, 1071, 1167
MvnI CGCG 2 cut(s) 199, 444
MwoI GCNNNNNNNGC 6 cut(s) 175, 450, 461, 959, 1142, 1393
NarI GGCGCC 1 cut(s) 453
NciI CCSGG 1 cut(s) 1201
NdeI CATATG 1 cut(s) 359
NdeII GATC 5 cut(s) 31, 70, 583, 1330, 1372
NlaIII CATG 4 cut(s) 188, 269, 695, 877
NlaIV GGNNCC 5 cut(s) 112, 454, 498, 510, 835
NmeAIII GCCGAG 2 cut(s) 197, 217
NmuCI GTSAC 2 cut(s) 235, 330
NruI TCGCGA 2 cut(s) 199, 444
NsbI TGCGCA 1 cut(s) 829
NspI RCATGY 1 cut(s) 695
PagI TCATGA 1 cut(s) 184
PceI AGGCCT 1 cut(s) 1208
PdmI GAANNNNTTC 1 cut(s) 249
PfeI GAWTC 3 cut(s) 446, 931, 1283
PflFI GACNNNGTC 1 cut(s) 622
PflMI CCANNNNNTGG 1 cut(s) 1076
PkrI GCNGC 8 cut(s) 637, 964, 1006, 1102, 1161, 1164, 1215, 1468
PleI GAGTC 9 cut(s) 44, 202, 209, 242, 427, 542, 613, 1298, 1452
PluTI GGCGCC 1 cut(s) 456
PpsI GAGTC 9 cut(s) 44, 202, 209, 242, 427, 542, 613, 1298, 1452
Psp124BI GAGCTC 2 cut(s) 998, 1389
Psp6I CCWGG 4 cut(s) 288, 363, 1069, 1165
PspGI CCWGG 4 cut(s) 288, 363, 1069, 1165
PspN4I GGNNCC 5 cut(s) 112, 454, 498, 510, 835
PspPI GGNCC 2 cut(s) 110, 496
PstNI CAGNNNCTG 2 cut(s) 632, 1004
PsyI GACNNNGTC 1 cut(s) 622
PvuII CAGCTG 1 cut(s) 917
RruI TCGCGA 2 cut(s) 199, 444
RsaI GTAC 5 cut(s) 55, 287, 350, 647, 950
RsaNI GTAC 5 cut(s) 54, 286, 349, 646, 949
RseI CAYNNNNRTG 2 cut(s) 779, 792
SacI GAGCTC 2 cut(s) 998, 1389
SaqAI TTAA 3 cut(s) 392, 521, 602
SatI GCNGC 8 cut(s) 636, 963, 1005, 1101, 1160, 1163, 1214, 1467
Sau3AI GATC 5 cut(s) 31, 70, 583, 1330, 1372
Sau96I GGNCC 2 cut(s) 110, 496
ScaI AGTACT 1 cut(s) 55
SchI GAGTC 9 cut(s) 45, 203, 209, 243, 428, 542, 613, 1298, 1453
ScrFI CCNGG 5 cut(s) 290, 365, 1071, 1167, 1201
SduI GDGCHC 3 cut(s) 998, 1250, 1389
SfaNI GCATC 4 cut(s) 840, 871, 1453, 1539
SfoI GGCGCC 1 cut(s) 454
SinI GGWCC 2 cut(s) 110, 496
SmiMI CAYNNNNRTG 2 cut(s) 779, 792
SpeI ACTAGT 1 cut(s) 940
Sse9I AATT 8 cut(s) 261, 378, 387, 491, 697, 1022, 1424, 1455
SseBI AGGCCT 1 cut(s) 1208
SsiI CCGC 7 cut(s) 24, 129, 176, 1045, 1143, 1214, 1421
SspDI GGCGCC 1 cut(s) 452
SspI AATATT 1 cut(s) 519
SspMI CTAG 1 cut(s) 941
SstI GAGCTC 2 cut(s) 998, 1389
StuI AGGCCT 1 cut(s) 1208
StyD4I CCNGG 5 cut(s) 288, 363, 1069, 1165, 1199
StyI CCWWGG 3 cut(s) 106, 814, 1052
TaaI ACNGT 5 cut(s) 567, 733, 844, 1011, 1479
TaqI TCGA 4 cut(s) 34, 69, 144, 1194
TasI AATT 8 cut(s) 261, 378, 387, 491, 697, 1022, 1424, 1455
TatI WGTACW 2 cut(s) 53, 645
TauI GCSGC 1 cut(s) 1216
TfiI GAWTC 3 cut(s) 446, 931, 1283
Tru1I TTAA 3 cut(s) 392, 521, 602
Tru9I TTAA 3 cut(s) 392, 521, 602
TscAI CASTG 3 cut(s) 375, 637, 847
TseFI GTSAC 2 cut(s) 235, 330
TseI GCWGC 7 cut(s) 635, 962, 1004, 1100, 1159, 1162, 1466
Tsp45I GTSAC 2 cut(s) 235, 330
TspDTI ATGAA 8 cut(s) 245, 254, 258, 459, 691, 763, 1102, 1354
TspGWI ACGGA 1 cut(s) 1100
TspRI CASTG 3 cut(s) 375, 637, 847
Tth111I GACNNNGTC 1 cut(s) 622
Van91I CCANNNNNTGG 1 cut(s) 1076
VpaK11BI GGWCC 2 cut(s) 110, 496
XapI RAATTY 2 cut(s) 697, 1455
XceI RCATGY 1 cut(s) 695
XmnI GAANNNNTTC 1 cut(s) 249
XspI CTAG 1 cut(s) 941
ZrmI AGTACT 1 cut(s) 55
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.