RLG00000029398

4-coumarate--CoA ligase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0005541
Physical Location & Seq
Reverse (-)
38044780 .. 38079720
34941 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000029398

Sequence Viewer

Length: 1374 bp
ATGTCTATGGAAGAACTATCCGGCGAGTACAGCGGCAGAACAAACGGTGGTTTTGATTCCCAAACCGGAGTATACCATTCCCTTTTTAACCTTGGTGACCGTCACAAAATCCCAACAAGGCCTAACCTTGACACTGCCACATTTGTGTTGTCACAGTTCCCGCACCCTCACCTGGCGGAGTCGCGAGTTGCGCTCATTGACTCGGCCACAAACCAGCGAGTCACTTATGCACAACTTCATCGATCTATACAGTCTCTTGCTTCAGGCTTGCACCAAGCACTTGGAGTTCAAAAAGGGGATGTGGTGTTTGTGTTATCACCAAACTCACTCCTATACCCAACAATATGTTTGGCAGTTTTTTCAGTTGGTGCAATACTAACTACTGCCAACCCACTCAACACCAAGTCAGAAATCGCCAAACAAGTCCGAGACTCAGGTGCCAAACTCGCTATTGCATCACCTGAGGAGGTTCACAAGTTGGTCCCAACTGGTGTGCCTACAATTGTAACATCAAGGCCATCAAATGGTACTGATAACTCACTCTCCATTGAAGAGTTGATTGAATCCTGTGATCCAATACCGACCGAGTTAGCACTAGCACGTCCAACTCAGTCGGACACAGCAGCCATATTGTACTCTTCGGGTACAACTGGGACAAGCAAAGGTGTAGTTTTGACACATGCAAATTTCATAGCCATCATGACATTGCTTAAATGGTCTGTGGATGAAACATCAGCTCAAGATGATGTGTTCTTATGCTTCATTCCTATGTTCCACATCTACGGGCTCGCTTTCTTTGCGCTTGGAATGTTTAGCACAGGATCCACCATTGTCCTGATGCAAAGGTTTGAGTTCAAGGCCATGTTGGATGCTATACAAACTCATAAAGTAAATAATATACCCGCAGTGCCTCCGGTGATTCTTGGACTAGTGAAGTATGCAAGCCAAGCCGGGTGCAAGTTGTCATCTTTGAGGAGGGTTGGTTCAGGAGCTGCACCATTGAGCAAAGAAGTGGTTGATGAGTTCAGACAGAAGTTTCCTTGGGTTGAGTTGAGGCCGGGGTATGGCCTAACCGAAAGTTGTGGTGCAGCAACTTTTTTTATTTCGGATGAACAAGCTAAGAAACACACTGGCTCATGTGGGCATCTGGTTCCAAAATTTACTGCTAAGGTTGTGGATATTGAAACTGGACAGGCTTTGGGACCTTATAAGGAAGGAGAGCTGTGGCTGAAGAGTCCGACTATCATGAAGGAGTATTTGGGGAATGTGGAAGCAACTATTGCTACTATTGATGAAGAGGGGTGGTTGAAAACTGGTGATCTTTGTTATTTTGATGAGGATGGACTGCTTTACATTGTTGATCGATCTCAGTAG

Protein Analysis

458

Amino Acids

49.55

Weight (kDa)

5.67

Isoelectric Point (pI)

40.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 61 - 420 1.3e-88 AMP-binding enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1209
AccB1I GGYRCC 1 cut(s) 437
AccB7I CCANNNNNTGG 1 cut(s) 524
AccI GTMKAC 1 cut(s) 72
AccII CGCG 1 cut(s) 184
AciI CCGC 4 cut(s) 33, 161, 176, 903
AclWI GGATC 3 cut(s) 566, 816, 829
AcoI YGGCCR 1 cut(s) 204
AcsI RAATTY 2 cut(s) 685, 1157
AcuI CTGAAG 2 cut(s) 246, 1250
AfaI GTAC 4 cut(s) 29, 529, 635, 646
AfiI CCNNNNNNNGG 3 cut(s) 172, 524, 1064
AgsI TTSAA 6 cut(s) 290, 551, 563, 856, 1184, 1309
AhlI ACTAGT 1 cut(s) 928
AjiI CACGTC 1 cut(s) 602
AjnI CCWGG 1 cut(s) 171
AjuI GAANNNNNNNTTGG 2 cut(s) 1241, 1273
AleI CACNNNNGTG 1 cut(s) 143
AloI GAACNNNNNNTCC 2 cut(s) 967, 999
AluBI AGCT 4 cut(s) 737, 992, 1118, 1222
AluI AGCT 4 cut(s) 737, 992, 1118, 1222
Alw26I GTCTC 2 cut(s) 258, 423
AlwI GGATC 3 cut(s) 566, 816, 829
AlwNI CAGNNNCTG 1 cut(s) 992
AoxI GGCC 6 cut(s) 119, 204, 515, 858, 1055, 1066
ApeKI GCWGC 3 cut(s) 623, 992, 1088
ApoI RAATTY 2 cut(s) 685, 1157
AspLEI GCGC 2 cut(s) 193, 802
AspS9I GGNCC 2 cut(s) 481, 1202
AsuC2I CCSGG 2 cut(s) 952, 1059
AsuHPI GGTGA 6 cut(s) 107, 161, 309, 450, 928, 1328
AvaII GGWCC 2 cut(s) 481, 1202
AxyI CCTNAGG 1 cut(s) 462
BamHI GGATCC 1 cut(s) 821
BanI GGYRCC 1 cut(s) 437
BanII GRGCYC 1 cut(s) 789
BbvI GCAGC 3 cut(s) 635, 979, 1100
BccI CCATC 3 cut(s) 526, 704, 1334
BciT130I CCWGG 1 cut(s) 173
BcnI CCSGG 2 cut(s) 952, 1059
BcoDI GTCTC 2 cut(s) 258, 423
BcuI ACTAGT 1 cut(s) 928
BfaI CTAG 2 cut(s) 596, 929
BisI GCNGC 4 cut(s) 34, 624, 993, 1089
BlsI GCNGC 4 cut(s) 35, 625, 994, 1090
Bme1390I CCNGG 3 cut(s) 173, 952, 1059
Bme18I GGWCC 2 cut(s) 481, 1202
BmgBI CACGTC 1 cut(s) 602
BmgT120I GGNCC 2 cut(s) 481, 1202
BmiI GGNNCC 5 cut(s) 439, 483, 823, 1152, 1203
BmrFI CCNGG 3 cut(s) 173, 952, 1059
BmrI ACTGGG 1 cut(s) 660
BmsI GCATC 4 cut(s) 464, 828, 859, 1153
BmuI ACTGGG 1 cut(s) 660
BplI GAGNNNNNCTC 2 cut(s) 177, 209
Bpu10I CCTNAGC 1 cut(s) 1167
BpuEI CTTGAG 1 cut(s) 723
BpuMI CCSGG 2 cut(s) 952, 1059
Bsa29I ATCGAT 2 cut(s) 241, 1363
BsaJI CCNNGG 3 cut(s) 91, 1040, 1058
BsaWI WCCGGW 2 cut(s) 65, 913
BsaXI ACNNNNNCTCC 4 cut(s) 527, 557, 967, 997
Bsc4I CCNNNNNNNGG 3 cut(s) 172, 524, 1064
Bse1I ACTGG 5 cut(s) 493, 655, 1135, 1192, 1318
Bse21I CCTNAGG 1 cut(s) 462
Bse3DI GCAATG 1 cut(s) 704
BseBI CCWGG 1 cut(s) 173
BseCI ATCGAT 2 cut(s) 241, 1363
BseDI CCNNGG 3 cut(s) 91, 1040, 1058
BseGI GGATG 5 cut(s) 304, 730, 874, 1114, 1345
BseLI CCNNNNNNNGG 3 cut(s) 172, 524, 1064
BseMI GCAATG 1 cut(s) 704
BseMII CTCAG 3 cut(s) 447, 453, 623
BseNI ACTGG 5 cut(s) 493, 655, 1135, 1192, 1318
BseRI GAGGAG 2 cut(s) 479, 988
BseXI GCAGC 3 cut(s) 635, 979, 1100
BsgI GTGCAG 2 cut(s) 978, 1107
Bsh1236I CGCG 1 cut(s) 184
Bsh1285I CGRYCG 1 cut(s) 585
BshFI GGCC 6 cut(s) 121, 206, 517, 860, 1057, 1068
BshNI GGYRCC 1 cut(s) 437
BshVI ATCGAT 2 cut(s) 241, 1363
BsiEI CGRYCG 1 cut(s) 585
BsiSI CCGG 5 cut(s) 21, 66, 914, 951, 1058
BslFI GGGAC 3 cut(s) 467, 667, 1215
BslI CCNNNNNNNGG 3 cut(s) 172, 524, 1064
BsmAI GTCTC 2 cut(s) 258, 423
BsmFI GGGAC 3 cut(s) 467, 667, 1215
BsnI GGCC 6 cut(s) 121, 206, 517, 860, 1057, 1068
Bsp1286I GDGCHC 1 cut(s) 789
Bsp143I GATC 6 cut(s) 242, 571, 821, 1318, 1360, 1364
Bsp68I TCGCGA 1 cut(s) 184
BspACI CCGC 4 cut(s) 33, 161, 176, 903
BspANI GGCC 6 cut(s) 121, 206, 517, 860, 1057, 1068
BspCNI CTCAG 3 cut(s) 446, 454, 622
BspDI ATCGAT 2 cut(s) 241, 1363
BspFNI CGCG 1 cut(s) 184
BspHI TCATGA 2 cut(s) 699, 1245
BspLI GGNNCC 5 cut(s) 439, 483, 823, 1152, 1203
BspPI GGATC 3 cut(s) 566, 816, 829
BspT107I GGYRCC 1 cut(s) 437
BsrDI GCAATG 1 cut(s) 704
BsrI ACTGG 5 cut(s) 493, 655, 1135, 1192, 1318
BssECI CCNNGG 3 cut(s) 91, 1040, 1058
BssMI GATC 6 cut(s) 242, 571, 821, 1318, 1360, 1364
BssNAI GTATAC 1 cut(s) 73
BssT1I CCWWGG 2 cut(s) 91, 1040
Bst1107I GTATAC 1 cut(s) 73
Bst2UI CCWGG 1 cut(s) 173
Bst4CI ACNGT 4 cut(s) 47, 101, 156, 252
Bst6I CTCTTC 4 cut(s) 546, 643, 1226, 1290
BstAPI GCANNNNNTGC 1 cut(s) 1280
BstC8I GCNNGC 3 cut(s) 269, 789, 943
BstDEI CTNAG 6 cut(s) 433, 462, 609, 1119, 1167, 1368
BstEII GGTNACC 1 cut(s) 95
BstF5I GGATG 5 cut(s) 304, 730, 874, 1114, 1345
BstFNI CGCG 1 cut(s) 184
BstHHI GCGC 2 cut(s) 193, 802
BstKTI GATC 6 cut(s) 245, 574, 824, 1321, 1363, 1367
BstMAI GTCTC 2 cut(s) 258, 423
BstMBI GATC 6 cut(s) 242, 571, 821, 1318, 1360, 1364
BstMCI CGRYCG 1 cut(s) 585
BstMWI GCNNNNNNNGC 6 cut(s) 30, 190, 446, 797, 947, 1280
BstNI CCWGG 1 cut(s) 173
BstNSI RCATGY 1 cut(s) 683
BstPI GGTNACC 1 cut(s) 95
BstSCI CCNGG 3 cut(s) 171, 950, 1057
BstUI CGCG 1 cut(s) 184
BstV1I GCAGC 3 cut(s) 635, 979, 1100
BstX2I RGATCY 1 cut(s) 821
BstXI CCANNNNNNTGG 1 cut(s) 281
BstYI RGATCY 1 cut(s) 821
BstZ17I GTATAC 1 cut(s) 73
Bsu15I ATCGAT 2 cut(s) 241, 1363
Bsu36I CCTNAGG 1 cut(s) 462
BsuRI GGCC 6 cut(s) 121, 206, 517, 860, 1057, 1068
BsuTUI ATCGAT 2 cut(s) 241, 1363
BtrI CACGTC 1 cut(s) 602
BtsCI GGATG 5 cut(s) 304, 730, 874, 1114, 1345
BtsI GCAGTG 2 cut(s) 132, 912
BtsIMutI CAGTG 3 cut(s) 132, 912, 1128
BtuMI TCGCGA 1 cut(s) 184
Cac8I GCNNGC 3 cut(s) 269, 789, 943
CaiI CAGNNNCTG 1 cut(s) 992
CciI TCATGA 2 cut(s) 699, 1245
CfoI GCGC 2 cut(s) 193, 802
Cfr13I GGNCC 2 cut(s) 481, 1202
ClaI ATCGAT 2 cut(s) 241, 1363
Csp6I GTAC 4 cut(s) 28, 528, 634, 645
CviAII CATG 5 cut(s) 680, 700, 862, 1137, 1246
CviQI GTAC 4 cut(s) 28, 528, 634, 645
DdeI CTNAG 6 cut(s) 433, 462, 609, 1119, 1167, 1368
DpnI GATC 6 cut(s) 244, 573, 823, 1320, 1362, 1366
DpnII GATC 6 cut(s) 242, 571, 821, 1318, 1360, 1364
EaeI YGGCCR 1 cut(s) 204
Eam1104I CTCTTC 4 cut(s) 546, 643, 1226, 1290
EarI CTCTTC 4 cut(s) 546, 643, 1226, 1290
EciI GGCGGA 1 cut(s) 191
Eco130I CCWWGG 2 cut(s) 91, 1040
Eco147I AGGCCT 1 cut(s) 121
Eco24I GRGCYC 1 cut(s) 789
Eco47I GGWCC 2 cut(s) 481, 1202
Eco57I CTGAAG 2 cut(s) 246, 1250
Eco81I CCTNAGG 1 cut(s) 462
Eco91I GGTNACC 1 cut(s) 95
EcoO109I RGGNCCY 1 cut(s) 1202
EcoO65I GGTNACC 1 cut(s) 95
EcoRII CCWGG 1 cut(s) 171
EcoT14I CCWWGG 2 cut(s) 91, 1040
EcoT38I GRGCYC 1 cut(s) 789
ErhI CCWWGG 2 cut(s) 91, 1040
FaeI CATG 5 cut(s) 683, 703, 865, 1140, 1249
FaqI GGGAC 3 cut(s) 467, 667, 1215
FatI CATG 5 cut(s) 679, 699, 861, 1136, 1245
FauI CCCGC 2 cut(s) 168, 910
FblI GTMKAC 1 cut(s) 72
Fnu4HI GCNGC 4 cut(s) 34, 624, 993, 1089
FokI GGATG 5 cut(s) 311, 737, 881, 1121, 1352
FriOI GRGCYC 1 cut(s) 789
Fsp4HI GCNGC 4 cut(s) 34, 624, 993, 1089
FspBI CTAG 2 cut(s) 596, 929
GlaI GCGC 2 cut(s) 192, 801
GluI GCNGC 4 cut(s) 34, 624, 993, 1089
HaeIII GGCC 6 cut(s) 121, 206, 517, 860, 1057, 1068
HapII CCGG 5 cut(s) 21, 66, 914, 951, 1058
HhaI GCGC 2 cut(s) 193, 802
Hin1II CATG 5 cut(s) 683, 703, 865, 1140, 1249
Hin6I GCGC 2 cut(s) 191, 800
HinP1I GCGC 2 cut(s) 191, 800
HinfI GANTC 8 cut(s) 56, 179, 200, 219, 431, 563, 919, 1234
HpaII CCGG 5 cut(s) 21, 66, 914, 951, 1058
HphI GGTGA 6 cut(s) 107, 161, 309, 450, 928, 1328
Hpy166II GTNNAC 2 cut(s) 73, 472
Hpy188I TCNGA 6 cut(s) 409, 428, 616, 1028, 1108, 1239
Hpy188III TCNNGA 6 cut(s) 183, 700, 740, 835, 987, 1246
Hpy8I GTNNAC 2 cut(s) 73, 472
HpyAV CCTTC 2 cut(s) 1208, 1243
HpyCH4III ACNGT 4 cut(s) 47, 101, 156, 252
HpyCH4IV ACGT 1 cut(s) 601
HpyF10VI GCNNNNNNNGC 6 cut(s) 30, 190, 446, 797, 947, 1280
HpyF3I CTNAG 6 cut(s) 433, 462, 609, 1119, 1167, 1368
HpySE526I ACGT 1 cut(s) 601
Hsp92II CATG 5 cut(s) 683, 703, 865, 1140, 1249
HspAI GCGC 2 cut(s) 191, 800
Kzo9I GATC 6 cut(s) 242, 571, 821, 1318, 1360, 1364
LmnI GCTCC 1 cut(s) 989
Lsp1109I GCAGC 3 cut(s) 635, 979, 1100
LweI GCATC 4 cut(s) 464, 828, 859, 1153
MaeI CTAG 2 cut(s) 596, 929
MaeII ACGT 1 cut(s) 601
MaeIII GTNAC 5 cut(s) 95, 101, 150, 220, 505
MalI GATC 6 cut(s) 244, 573, 823, 1320, 1362, 1366
MboI GATC 6 cut(s) 242, 571, 821, 1318, 1360, 1364
MboII GAAGA 5 cut(s) 23, 563, 630, 1243, 1307
MfeI CAATTG 1 cut(s) 501
MflI RGATCY 1 cut(s) 821
MhlI GDGCHC 1 cut(s) 789
MluCI AATT 3 cut(s) 501, 685, 1157
MlyI GAGTC 5 cut(s) 188, 194, 228, 425, 1243
MmeI TCCRAC 4 cut(s) 594, 629, 846, 1262
MnlI CCTC 9 cut(s) 177, 457, 460, 921, 966, 969, 1047, 1291, 1330
MseI TTAA 2 cut(s) 87, 711
MslI CAYNNNNRTG 2 cut(s) 143, 767
MspA1I CMGCKG 1 cut(s) 33
MspI CCGG 5 cut(s) 21, 66, 914, 951, 1058
MspR9I CCNGG 3 cut(s) 173, 952, 1059
MunI CAATTG 1 cut(s) 501
MvaI CCWGG 1 cut(s) 173
MvnI CGCG 1 cut(s) 184
MwoI GCNNNNNNNGC 6 cut(s) 30, 190, 446, 797, 947, 1280
NciI CCSGG 2 cut(s) 952, 1059
NdeII GATC 6 cut(s) 242, 571, 821, 1318, 1360, 1364
NlaIII CATG 5 cut(s) 683, 703, 865, 1140, 1249
NlaIV GGNNCC 5 cut(s) 439, 483, 823, 1152, 1203
NmeAIII GCCGAG 1 cut(s) 182
NmuCI GTSAC 4 cut(s) 95, 101, 150, 220
NruI TCGCGA 1 cut(s) 184
NspI RCATGY 1 cut(s) 683
OliI CACNNNNGTG 1 cut(s) 143
PagI TCATGA 2 cut(s) 699, 1245
PceI AGGCCT 1 cut(s) 121
PfeI GAWTC 3 cut(s) 56, 563, 919
PflMI CCANNNNNTGG 1 cut(s) 524
PkrI GCNGC 4 cut(s) 35, 625, 994, 1090
PleI GAGTC 5 cut(s) 187, 194, 227, 425, 1242
PpsI GAGTC 5 cut(s) 187, 194, 227, 425, 1242
PpuMI RGGWCCY 1 cut(s) 1202
PsiI TTATAA 1 cut(s) 1209
Psp5II RGGWCCY 1 cut(s) 1202
Psp6I CCWGG 1 cut(s) 171
PspEI GGTNACC 1 cut(s) 95
PspGI CCWGG 1 cut(s) 171
PspN4I GGNNCC 5 cut(s) 439, 483, 823, 1152, 1203
PspPI GGNCC 2 cut(s) 481, 1202
PspPPI RGGWCCY 1 cut(s) 1202
PstNI CAGNNNCTG 1 cut(s) 992
PsuI RGATCY 1 cut(s) 821
RruI TCGCGA 1 cut(s) 184
RsaI GTAC 4 cut(s) 29, 529, 635, 646
RsaNI GTAC 4 cut(s) 28, 528, 634, 645
RseI CAYNNNNRTG 2 cut(s) 143, 767
SaqAI TTAA 2 cut(s) 87, 711
SatI GCNGC 4 cut(s) 34, 624, 993, 1089
Sau3AI GATC 6 cut(s) 242, 571, 821, 1318, 1360, 1364
Sau96I GGNCC 2 cut(s) 481, 1202
SchI GAGTC 5 cut(s) 188, 194, 228, 425, 1243
ScrFI CCNGG 3 cut(s) 173, 952, 1059
SduI GDGCHC 1 cut(s) 789
SfaNI GCATC 4 cut(s) 464, 828, 859, 1153
SinI GGWCC 2 cut(s) 481, 1202
SmiMI CAYNNNNRTG 2 cut(s) 143, 767
SmlI CTYRAG 1 cut(s) 738
SmoI CTYRAG 1 cut(s) 738
SpeI ACTAGT 1 cut(s) 928
Sse9I AATT 3 cut(s) 501, 685, 1157
SseBI AGGCCT 1 cut(s) 121
SsiI CCGC 4 cut(s) 33, 161, 176, 903
SspMI CTAG 2 cut(s) 596, 929
StuI AGGCCT 1 cut(s) 121
StyD4I CCNGG 3 cut(s) 171, 950, 1057
StyI CCWWGG 2 cut(s) 91, 1040
TaaI ACNGT 4 cut(s) 47, 101, 156, 252
TaiI ACGT 1 cut(s) 604
TaqI TCGA 2 cut(s) 241, 1363
TaqII GACCGA 1 cut(s) 599
TasI AATT 3 cut(s) 501, 685, 1157
TatI WGTACW 2 cut(s) 27, 633
TauI GCSGC 1 cut(s) 36
TfiI GAWTC 3 cut(s) 56, 563, 919
Tru1I TTAA 2 cut(s) 87, 711
Tru9I TTAA 2 cut(s) 87, 711
TscAI CASTG 3 cut(s) 139, 912, 1135
TseFI GTSAC 4 cut(s) 95, 101, 150, 220
TseI GCWGC 3 cut(s) 623, 992, 1088
Tsp45I GTSAC 4 cut(s) 95, 101, 150, 220
TspDTI ATGAA 7 cut(s) 227, 679, 741, 751, 1125, 1262, 1308
TspRI CASTG 3 cut(s) 139, 912, 1135
Van91I CCANNNNNTGG 1 cut(s) 524
VpaK11BI GGWCC 2 cut(s) 481, 1202
XapI RAATTY 2 cut(s) 685, 1157
XceI RCATGY 1 cut(s) 683
XmiI GTMKAC 1 cut(s) 72
XspI CTAG 2 cut(s) 596, 929
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.