Rh1DG148100

4-coumarate--CoA ligase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0005541
Physical Location & Seq
Forward (+)
30973937 .. 30974459
523 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG148100.1

Sequence Viewer

Length: 324 bp
ATGGTCAGGAGAGCACCAAATAAGCGTCCACGAAGGGTTGTTGACATACCCGTTGTGACCCCGATGAGTCTTGGACTAGTGAAGTATGCAGGTCAAGCCTGGTGTGACCTATCATCCTTGAGGAGGGTCAGTTCAGGAGCTGCACCATTGAGCAAAGAAGTGGCTGATGAGTTCAGACTGAAGTTTCCTTGGGTTGAGTTGAAGCCGGGGTATGGCCTAACTGAAAGTTTCGGGGCAGCAACTTTCTTTATTTCCAATGAACATGACACACCGACTCATGTGGGCAGGCTTTGGAACCTTATAAGGAAGGAGAGTTGTGGCTGA

Protein Analysis

107

Amino Acids

11.97

Weight (kDa)

9.82

Isoelectric Point (pI)

52.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 17 - 97 6.3e-11 AMP-binding enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 302
Acc36I ACCTGC 1 cut(s) 80
AcuI CTGAAG 1 cut(s) 200
AfiI CCNNNNNNNGG 2 cut(s) 123, 212
AgsI TTSAA 1 cut(s) 202
AhlI ACTAGT 1 cut(s) 76
AjnI CCWGG 1 cut(s) 98
AloI GAACNNNNNNTCC 2 cut(s) 115, 147
AluBI AGCT 1 cut(s) 140
AluI AGCT 1 cut(s) 140
Alw21I GWGCWC 1 cut(s) 16
AlwNI CAGNNNCTG 1 cut(s) 140
AoxI GGCC 1 cut(s) 214
ApeKI GCWGC 2 cut(s) 140, 236
AsuC2I CCSGG 1 cut(s) 207
Bbv12I GWGCWC 1 cut(s) 16
BbvI GCAGC 2 cut(s) 127, 248
BciT130I CCWGG 1 cut(s) 100
BcnI CCSGG 1 cut(s) 207
BcuI ACTAGT 1 cut(s) 76
BfaI CTAG 1 cut(s) 77
BfuAI ACCTGC 1 cut(s) 80
BisI GCNGC 2 cut(s) 141, 237
BlsI GCNGC 2 cut(s) 142, 238
Bme1390I CCNGG 2 cut(s) 100, 207
BmiI GGNNCC 1 cut(s) 296
BmrFI CCNGG 2 cut(s) 100, 207
BpuEI CTTGAG 1 cut(s) 139
BpuMI CCSGG 1 cut(s) 207
BsaJI CCNNGG 2 cut(s) 188, 206
BsaXI ACNNNNNCTCC 2 cut(s) 115, 145
Bsc4I CCNNNNNNNGG 2 cut(s) 123, 212
BseBI CCWGG 1 cut(s) 100
BseDI CCNNGG 2 cut(s) 188, 206
BseGI GGATG 1 cut(s) 113
BseLI CCNNNNNNNGG 2 cut(s) 123, 212
BseRI GAGGAG 1 cut(s) 136
BseXI GCAGC 2 cut(s) 127, 248
BsgI GTGCAG 1 cut(s) 126
BshFI GGCC 1 cut(s) 216
BsiHKAI GWGCWC 1 cut(s) 16
BsiSI CCGG 1 cut(s) 206
BslI CCNNNNNNNGG 2 cut(s) 123, 212
BsnI GGCC 1 cut(s) 216
Bsp1286I GDGCHC 1 cut(s) 16
BspANI GGCC 1 cut(s) 216
BspLI GGNNCC 1 cut(s) 296
BspMI ACCTGC 1 cut(s) 80
BssECI CCNNGG 2 cut(s) 188, 206
BssT1I CCWWGG 1 cut(s) 188
Bst2UI CCWGG 1 cut(s) 100
BstC8I GCNNGC 1 cut(s) 287
BstENI CCTNNNNNAGG 1 cut(s) 121
BstF5I GGATG 1 cut(s) 113
BstMWI GCNNNNNNNGC 1 cut(s) 95
BstNI CCWGG 1 cut(s) 100
BstSCI CCNGG 2 cut(s) 98, 205
BstV1I GCAGC 2 cut(s) 127, 248
BsuRI GGCC 1 cut(s) 216
BtsCI GGATG 1 cut(s) 113
BveI ACCTGC 1 cut(s) 80
Cac8I GCNNGC 1 cut(s) 287
CaiI CAGNNNCTG 1 cut(s) 140
CseI GACGC 1 cut(s) 14
CviAII CATG 2 cut(s) 263, 278
CviJI RGCY 7 cut(s) 98, 140, 164, 205, 216, 289, 321
CviKI_1 RGCY 7 cut(s) 98, 140, 164, 205, 216, 289, 321
Eco130I CCWWGG 1 cut(s) 188
Eco57I CTGAAG 1 cut(s) 200
EcoNI CCTNNNNNAGG 1 cut(s) 121
EcoRII CCWGG 1 cut(s) 98
EcoT14I CCWWGG 1 cut(s) 188
ErhI CCWWGG 1 cut(s) 188
FaeI CATG 2 cut(s) 266, 281
FaiI YATR 6 cut(s) 47, 87, 213, 264, 279, 302
FatI CATG 2 cut(s) 262, 277
Fnu4HI GCNGC 2 cut(s) 141, 237
FokI GGATG 1 cut(s) 100
Fsp4HI GCNGC 2 cut(s) 141, 237
FspBI CTAG 1 cut(s) 77
GluI GCNGC 2 cut(s) 141, 237
HaeIII GGCC 1 cut(s) 216
HapII CCGG 1 cut(s) 206
HgaI GACGC 1 cut(s) 14
Hin1II CATG 2 cut(s) 266, 281
HincII GTYRAC 1 cut(s) 43
HindII GTYRAC 1 cut(s) 43
HinfI GANTC 2 cut(s) 67, 274
HpaII CCGG 1 cut(s) 206
Hpy166II GTNNAC 2 cut(s) 29, 43
Hpy188I TCNGA 1 cut(s) 176
Hpy188III TCNNGA 2 cut(s) 7, 135
Hpy8I GTNNAC 2 cut(s) 29, 43
HpyAV CCTTC 2 cut(s) 27, 301
HpyCH4V TGCA 2 cut(s) 89, 143
HpyF10VI GCNNNNNNNGC 1 cut(s) 95
Hsp92II CATG 2 cut(s) 266, 281
LmnI GCTCC 1 cut(s) 137
LpnPI CCDG 6 cut(s) 75, 85, 112, 120, 219, 271
Lsp1109I GCAGC 2 cut(s) 127, 248
MaeI CTAG 1 cut(s) 77
MaeIII GTNAC 2 cut(s) 55, 104
MhlI GDGCHC 1 cut(s) 16
MlyI GAGTC 2 cut(s) 76, 268
MnlI CCTC 2 cut(s) 114, 117
MspI CCGG 1 cut(s) 206
MspR9I CCNGG 2 cut(s) 100, 207
MvaI CCWGG 1 cut(s) 100
MwoI GCNNNNNNNGC 1 cut(s) 95
NciI CCSGG 1 cut(s) 207
NlaIII CATG 2 cut(s) 266, 281
NlaIV GGNNCC 1 cut(s) 296
NmuCI GTSAC 2 cut(s) 55, 104
PkrI GCNGC 2 cut(s) 142, 238
PleI GAGTC 2 cut(s) 75, 268
PpsI GAGTC 2 cut(s) 75, 268
PsiI TTATAA 1 cut(s) 302
Psp6I CCWGG 1 cut(s) 98
PspGI CCWGG 1 cut(s) 98
PspN4I GGNNCC 1 cut(s) 296
PstNI CAGNNNCTG 1 cut(s) 140
SatI GCNGC 2 cut(s) 141, 237
SchI GAGTC 2 cut(s) 76, 268
ScrFI CCNGG 2 cut(s) 100, 207
SduI GDGCHC 1 cut(s) 16
SetI ASST 4 cut(s) 94, 111, 142, 300
SmlI CTYRAG 1 cut(s) 118
SmoI CTYRAG 1 cut(s) 118
SpeI ACTAGT 1 cut(s) 76
SspMI CTAG 1 cut(s) 77
StyD4I CCNGG 2 cut(s) 98, 205
StyI CCWWGG 1 cut(s) 188
TseFI GTSAC 2 cut(s) 55, 104
TseI GCWGC 2 cut(s) 140, 236
Tsp45I GTSAC 2 cut(s) 55, 104
TspDTI ATGAA 1 cut(s) 273
XagI CCTNNNNNAGG 1 cut(s) 121
XspI CTAG 1 cut(s) 77
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.