MD02G1266600.v1.1

Type I inositol 1,4,5-trisphosphate 5-phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0003538
Physical Location & Seq
Reverse (-)
32068417 .. 32072308
3892 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1266600.v1.1.491

Sequence Viewer

Length: 1479 bp
ATGCCTGTCAGCAATCAAGACGTGAAAAAGAAGTCTTTTATTCATAAAATCTTCACCACAAAGGAGAAAGAAGGAAGCGGCTCTTCGGATTTCTCAGAAGCACGGCAGTCTAACCCATCAGTTGACATTCAATCTATAAGTGACACAGGATCTCAAATGCCTGCCGGTCAATATGTTCAGTCCTTTCGGGTTTTTGTGGCGACATGGAATGCAGGAGGGAAATCTCCTCATAGTGGCCTTAATCTGGATGATTTTCTGCAGGTTGATAATGAATCAGACATATATGTCTTGGGTTTCCAGGAAATTGTTCCGCTAAATGCAGGAAATGTACTAGTTCTGGAAGATAATGAACCTGCAGCAAAATGGCTCGCTTTAATCAATCAGTCGCTAAACAAATCACCTGATGGGTGTTCAAGAAGCAAAGCAGGTGGTTCACGATTTTCCCCGAAGCCTTCCCTTAAAAAGGTCAGTAAGACTTTTAGGACAGAGAGCAAACGGAGGTTGAAGAGTTGCAACTGCCCTTTTGAACTAGAAAGGAAACATAGTAAAGATTTTTGTTTTCGGTGCCAACAACCAAACATAAATGAAGACGACCTTTCTTCAGATCCAGATGAGGATGGATCTAATGCCTTTGATATATCGGAAATTTCCATGGCCTCTACTACCAACCAGATGAAGTACGGCCTTATAGCAAGTAAGCAAATGGTAGGAATTTTTGTTACTATTTGGGCGAGGAAAGAGTATATACAATATGTTAGCCACTTGAGGATCTCTTGCATCAGTCGCGGGATAATGGGACTTGGAAACAAGGGATGTATATCTGTGAGCTTCTTATTCCATCAGACAAGCTTTTGCTTTGTCTGCAGTCACTTGGCATCTGGGGAGAAAGAGGGCGATGAGCTTAGGAGAAATCTGGATGTTTTAGAGATTCTTAAAAGCACTCAGTTTCCGAAGATTTGCAAAACAGCTCGTAGTAGGATGCCGGATAAAATTCTAGGACATGATGTGGTCATATGGTTAGGGGACTTGAATTACCGCATTGCTTTGAGCTACTCTGAAACCCAAAAGCTATTGATGGACAACAACTGGGATGCACTTCTTGACAAAGATCAGCTCAAGATCGAAAGAGAAGCAGGGCGAGTATTCAAAGGATGGAAAGAGGGGAAAATCTACTTTGCACCTACTTACAAATACTTTTATAACTCAGACACATATTTTGGAGAGATAAAAACATCGAAAAAGAAAAGAAGAACACCAGCTTGGTGTGATAGAATACTATGGCATGGAAATGGGATCAGACAACTTTCTTACATACGTGGAGAGTCTCGGTTCTCTGACCATCGGCCAGTGTGTGCAGCATTTTTTGTGGATGTTGTAGTTAATGAGAATGAAAATAAGAGGGGATCACCTAGCTCTAATATGAAAATCGAAATTGAAGAGCTTTTACCAACTGCTAGATACCAGAGAAACATGTACTAA

Protein Analysis

493

Amino Acids

55.9

Weight (kDa)

8.88

Isoelectric Point (pI)

48.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos2 PF22669 222 - 452 1.2e-60 Endonuclease/Exonuclease/phosphatase family 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1200
AarI CACCTGC 1 cut(s) 416
AasI GACNNNNNNGTC 1 cut(s) 284
Acc36I ACCTGC 3 cut(s) 250, 361, 416
AccB1I GGYRCC 1 cut(s) 564
AccII CGCG 1 cut(s) 786
AciI CCGC 4 cut(s) 78, 311, 786, 1036
AclWI GGATC 6 cut(s) 157, 599, 628, 776, 1301, 1411
AcoI YGGCCR 1 cut(s) 1343
AcsI RAATTY 3 cut(s) 645, 711, 990
AcuI CTGAAG 1 cut(s) 585
AfaI GTAC 3 cut(s) 330, 680, 1475
AfiI CCNNNNNNNGG 3 cut(s) 233, 244, 463
AflIII ACRYGT 1 cut(s) 1470
AgsI TTSAA 7 cut(s) 131, 414, 505, 527, 1030, 1147, 1436
AhlI ACTAGT 1 cut(s) 331
AjiI CACGTC 1 cut(s) 22
AjnI CCWGG 1 cut(s) 297
AjuI GAANNNNNNNTTGG 2 cut(s) 1243, 1275
Alw26I GTCTC 1 cut(s) 1329
AlwI GGATC 6 cut(s) 157, 599, 628, 776, 1301, 1411
AoxI GGCC 4 cut(s) 235, 654, 682, 1343
ApeKI GCWGC 2 cut(s) 356, 1355
ApoI RAATTY 3 cut(s) 645, 711, 990
ArsI GACNNNNNNTTYG 2 cut(s) 1199, 1231
Asp700I GAANNNNTTC 1 cut(s) 306
AsuHPI GGTGA 3 cut(s) 46, 390, 1398
BanI GGYRCC 1 cut(s) 564
BarI GAAGNNNNNNTAC 2 cut(s) 1428, 1460
BbsI GAAGAC 1 cut(s) 594
BbvI GCAGC 2 cut(s) 368, 1367
BccI CCATC 7 cut(s) 124, 398, 611, 846, 1069, 1146, 1347
BceAI ACGGC 2 cut(s) 119, 697
BciT130I CCWGG 1 cut(s) 299
BcoDI GTCTC 1 cut(s) 1329
BcuI ACTAGT 1 cut(s) 331
BfaI CTAG 5 cut(s) 332, 530, 995, 1410, 1455
BfmI CTRYAG 3 cut(s) 257, 354, 862
BfuAI ACCTGC 3 cut(s) 250, 361, 416
BisI GCNGC 3 cut(s) 79, 357, 1356
BlsI GCNGC 3 cut(s) 80, 358, 1357
Bme1390I CCNGG 1 cut(s) 299
BmgBI CACGTC 1 cut(s) 22
BmiI GGNNCC 1 cut(s) 566
BmrFI CCNGG 1 cut(s) 299
BmrI ACTGGG 1 cut(s) 1096
BmsI GCATC 4 cut(s) 786, 884, 969, 1081
BmuI ACTGGG 1 cut(s) 1096
BpiI GAAGAC 1 cut(s) 594
Bpu10I CCTNAGC 1 cut(s) 902
BpuEI CTTGAG 2 cut(s) 784, 1100
BsaAI YACGTR 1 cut(s) 1316
BsaBI GATNNNNATC 1 cut(s) 817
BsaJI CCNNGG 1 cut(s) 651
Bsc4I CCNNNNNNNGG 3 cut(s) 233, 244, 463
Bse118I RCCGGY 1 cut(s) 164
Bse1I ACTGG 2 cut(s) 1091, 1346
Bse3DI GCAATG 1 cut(s) 1038
Bse8I GATNNNNATC 1 cut(s) 817
BseBI CCWGG 1 cut(s) 299
BseDI CCNNGG 1 cut(s) 651
BseGI GGATG 8 cut(s) 253, 622, 818, 922, 984, 1096, 1157, 1375
BseJI GATNNNNATC 1 cut(s) 817
BseLI CCNNNNNNNGG 3 cut(s) 233, 244, 463
BseMI GCAATG 1 cut(s) 1038
BseMII CTCAG 3 cut(s) 108, 956, 1218
BseNI ACTGG 2 cut(s) 1091, 1346
BseRI GAGGAG 1 cut(s) 216
BseXI GCAGC 2 cut(s) 368, 1367
BsgI GTGCAG 1 cut(s) 1374
Bsh1236I CGCG 1 cut(s) 786
BshFI GGCC 4 cut(s) 237, 656, 684, 1345
BshNI GGYRCC 1 cut(s) 564
BsiSI CCGG 2 cut(s) 165, 983
BslFI GGGAC 2 cut(s) 810, 1037
BslI CCNNNNNNNGG 3 cut(s) 233, 244, 463
BsmAI GTCTC 1 cut(s) 1329
BsmFI GGGAC 2 cut(s) 810, 1037
BsmI GAATGC 1 cut(s) 214
BsnI GGCC 4 cut(s) 237, 656, 684, 1345
Bsp143I GATC 8 cut(s) 149, 604, 620, 768, 1108, 1119, 1293, 1403
Bsp19I CCATGG 1 cut(s) 651
BspACI CCGC 4 cut(s) 78, 311, 786, 1036
BspANI GGCC 4 cut(s) 237, 656, 684, 1345
BspCNI CTCAG 3 cut(s) 107, 955, 1217
BspFNI CGCG 1 cut(s) 786
BspLI GGNNCC 1 cut(s) 566
BspMAI CTGCAG 3 cut(s) 261, 358, 866
BspMI ACCTGC 3 cut(s) 250, 361, 416
BspPI GGATC 6 cut(s) 157, 599, 628, 776, 1301, 1411
BspQI GCTCTTC 2 cut(s) 88, 1431
BspT107I GGYRCC 1 cut(s) 564
BsrDI GCAATG 1 cut(s) 1038
BsrFI RCCGGY 1 cut(s) 164
BsrI ACTGG 2 cut(s) 1091, 1346
BssAI RCCGGY 1 cut(s) 164
BssECI CCNNGG 1 cut(s) 651
BssMI GATC 8 cut(s) 149, 604, 620, 768, 1108, 1119, 1293, 1403
BssT1I CCWWGG 1 cut(s) 651
Bst2UI CCWGG 1 cut(s) 299
Bst6I CTCTTC 3 cut(s) 88, 500, 1431
BstBAI YACGTR 1 cut(s) 1316
BstC8I GCNNGC 2 cut(s) 162, 369
BstDEI CTNAG 4 cut(s) 94, 902, 942, 1204
BstDSI CCRYGG 1 cut(s) 651
BstENI CCTNNNNNAGG 1 cut(s) 461
BstF5I GGATG 8 cut(s) 253, 622, 818, 922, 984, 1096, 1157, 1375
BstFNI CGCG 1 cut(s) 786
BstKTI GATC 8 cut(s) 152, 607, 623, 771, 1111, 1122, 1296, 1406
BstMAI GTCTC 1 cut(s) 1329
BstMBI GATC 8 cut(s) 149, 604, 620, 768, 1108, 1119, 1293, 1403
BstMWI GCNNNNNNNGC 2 cut(s) 783, 861
BstNI CCWGG 1 cut(s) 299
BstNSI RCATGY 1 cut(s) 1474
BstSCI CCNGG 1 cut(s) 297
BstSFI CTRYAG 3 cut(s) 257, 354, 862
BstUI CGCG 1 cut(s) 786
BstV1I GCAGC 2 cut(s) 368, 1367
BstV2I GAAGAC 1 cut(s) 594
BstX2I RGATCY 4 cut(s) 149, 604, 620, 768
BstYI RGATCY 4 cut(s) 149, 604, 620, 768
BsuRI GGCC 4 cut(s) 237, 656, 684, 1345
BtgI CCRYGG 1 cut(s) 651
BtgZI GCGATG 1 cut(s) 909
BtrI CACGTC 1 cut(s) 22
BtsCI GGATG 8 cut(s) 253, 622, 818, 922, 984, 1096, 1157, 1375
BtsIMutI CAGTG 1 cut(s) 1353
BveI ACCTGC 3 cut(s) 250, 361, 416
Cac8I GCNNGC 2 cut(s) 162, 369
Cfr10I RCCGGY 1 cut(s) 164
Csp6I GTAC 3 cut(s) 329, 679, 1474
CspCI CAANNNNNGTGG 2 cut(s) 409, 444
CviAII CATG 5 cut(s) 204, 652, 1001, 1283, 1471
CviQI GTAC 3 cut(s) 329, 679, 1474
DdeI CTNAG 4 cut(s) 94, 902, 942, 1204
DpnI GATC 8 cut(s) 151, 606, 622, 770, 1110, 1121, 1295, 1405
DpnII GATC 8 cut(s) 149, 604, 620, 768, 1108, 1119, 1293, 1403
DrdI GACNNNNNNGTC 1 cut(s) 284
DseDI GACNNNNNNGTC 1 cut(s) 284
EaeI YGGCCR 1 cut(s) 1343
Eam1104I CTCTTC 3 cut(s) 88, 500, 1431
EarI CTCTTC 3 cut(s) 88, 500, 1431
Eco130I CCWWGG 1 cut(s) 651
Eco57I CTGAAG 1 cut(s) 585
EcoNI CCTNNNNNAGG 1 cut(s) 461
EcoRII CCWGG 1 cut(s) 297
EcoT14I CCWWGG 1 cut(s) 651
ErhI CCWWGG 1 cut(s) 651
FaeI CATG 5 cut(s) 207, 655, 1004, 1286, 1474
FalI AAGNNNNNCTT 4 cut(s) 67, 99, 579, 611
FaqI GGGAC 2 cut(s) 810, 1037
FatI CATG 5 cut(s) 203, 651, 1000, 1282, 1470
FauI CCCGC 1 cut(s) 779
FauNDI CATATG 1 cut(s) 1013
Fnu4HI GCNGC 3 cut(s) 79, 357, 1356
FokI GGATG 8 cut(s) 260, 629, 825, 929, 991, 1103, 1164, 1382
Fsp4HI GCNGC 3 cut(s) 79, 357, 1356
FspBI CTAG 5 cut(s) 332, 530, 995, 1410, 1455
GluI GCNGC 3 cut(s) 79, 357, 1356
HaeIII GGCC 4 cut(s) 237, 656, 684, 1345
HapII CCGG 2 cut(s) 165, 983
Hin1II CATG 5 cut(s) 207, 655, 1004, 1286, 1474
HincII GTYRAC 1 cut(s) 124
HindII GTYRAC 1 cut(s) 124
HindIII AAGCTT 1 cut(s) 847
HinfI GANTC 3 cut(s) 272, 928, 1322
HpaII CCGG 2 cut(s) 165, 983
HphI GGTGA 3 cut(s) 46, 390, 1398
Hpy166II GTNNAC 2 cut(s) 124, 434
Hpy188III TCNNGA 9 cut(s) 17, 245, 338, 414, 435, 608, 914, 1100, 1117
Hpy8I GTNNAC 2 cut(s) 124, 434
HpyAV CCTTC 2 cut(s) 65, 462
HpyCH4IV ACGT 2 cut(s) 21, 1315
HpyF10VI GCNNNNNNNGC 2 cut(s) 783, 861
HpyF3I CTNAG 4 cut(s) 94, 902, 942, 1204
HpySE526I ACGT 2 cut(s) 21, 1315
Hsp92II CATG 5 cut(s) 207, 655, 1004, 1286, 1474
Kzo9I GATC 8 cut(s) 149, 604, 620, 768, 1108, 1119, 1293, 1403
LguI GCTCTTC 2 cut(s) 88, 1431
Lsp1109I GCAGC 2 cut(s) 368, 1367
LweI GCATC 4 cut(s) 786, 884, 969, 1081
MaeI CTAG 5 cut(s) 332, 530, 995, 1410, 1455
MaeII ACGT 2 cut(s) 21, 1315
MaeIII GTNAC 3 cut(s) 140, 718, 866
MalI GATC 8 cut(s) 151, 606, 622, 770, 1110, 1121, 1295, 1405
MboI GATC 8 cut(s) 149, 604, 620, 768, 1108, 1119, 1293, 1403
MboII GAAGA 9 cut(s) 43, 75, 353, 517, 591, 599, 964, 1260, 1448
MflI RGATCY 4 cut(s) 149, 604, 620, 768
MluCI AATT 6 cut(s) 303, 645, 711, 990, 1030, 1431
MlyI GAGTC 1 cut(s) 1331
MroXI GAANNNNTTC 1 cut(s) 306
MseI TTAA 5 cut(s) 240, 374, 459, 933, 1380
MslI CAYNNNNRTG 1 cut(s) 1287
MspI CCGG 2 cut(s) 165, 983
MspR9I CCNGG 1 cut(s) 299
Mva1269I GAATGC 1 cut(s) 214
MvaI CCWGG 1 cut(s) 299
MvnI CGCG 1 cut(s) 786
MwoI GCNNNNNNNGC 2 cut(s) 783, 861
NcoI CCATGG 1 cut(s) 651
NdeI CATATG 1 cut(s) 1013
NdeII GATC 8 cut(s) 149, 604, 620, 768, 1108, 1119, 1293, 1403
NlaIII CATG 5 cut(s) 207, 655, 1004, 1286, 1474
NlaIV GGNNCC 1 cut(s) 566
NmuCI GTSAC 2 cut(s) 140, 866
NspI RCATGY 1 cut(s) 1474
PaqCI CACCTGC 1 cut(s) 416
PciI ACATGT 1 cut(s) 1470
PciSI GCTCTTC 2 cut(s) 88, 1431
PctI GAATGC 1 cut(s) 214
PdmI GAANNNNTTC 1 cut(s) 306
PfeI GAWTC 2 cut(s) 272, 928
PfoI TCCNGGA 1 cut(s) 297
PkrI GCNGC 3 cut(s) 80, 358, 1357
PleI GAGTC 1 cut(s) 1330
PpsI GAGTC 1 cut(s) 1330
Ppu21I YACGTR 1 cut(s) 1316
PscI ACATGT 1 cut(s) 1470
PsiI TTATAA 1 cut(s) 1200
Psp6I CCWGG 1 cut(s) 297
PspGI CCWGG 1 cut(s) 297
PspN4I GGNNCC 1 cut(s) 566
PstI CTGCAG 3 cut(s) 261, 358, 866
PsuI RGATCY 4 cut(s) 149, 604, 620, 768
RsaI GTAC 3 cut(s) 330, 680, 1475
RsaNI GTAC 3 cut(s) 329, 679, 1474
RseI CAYNNNNRTG 1 cut(s) 1287
SapI GCTCTTC 2 cut(s) 88, 1431
SaqAI TTAA 5 cut(s) 240, 374, 459, 933, 1380
SatI GCNGC 3 cut(s) 79, 357, 1356
Sau3AI GATC 8 cut(s) 149, 604, 620, 768, 1108, 1119, 1293, 1403
SchI GAGTC 1 cut(s) 1331
ScrFI CCNGG 1 cut(s) 299
SfaNI GCATC 4 cut(s) 786, 884, 969, 1081
SfcI CTRYAG 3 cut(s) 257, 354, 862
SmiMI CAYNNNNRTG 1 cut(s) 1287
SmlI CTYRAG 2 cut(s) 763, 1115
SmoI CTYRAG 2 cut(s) 763, 1115
SpeI ACTAGT 1 cut(s) 331
Sse9I AATT 6 cut(s) 303, 645, 711, 990, 1030, 1431
SsiI CCGC 4 cut(s) 78, 311, 786, 1036
SspMI CTAG 5 cut(s) 332, 530, 995, 1410, 1455
StyD4I CCNGG 1 cut(s) 297
StyI CCWWGG 1 cut(s) 651
TaiI ACGT 2 cut(s) 24, 1318
TaqI TCGA 3 cut(s) 1122, 1235, 1428
TasI AATT 6 cut(s) 303, 645, 711, 990, 1030, 1431
TatI WGTACW 2 cut(s) 328, 1473
TauI GCSGC 1 cut(s) 81
TfiI GAWTC 2 cut(s) 272, 928
Tru1I TTAA 5 cut(s) 240, 374, 459, 933, 1380
Tru9I TTAA 5 cut(s) 240, 374, 459, 933, 1380
TscAI CASTG 1 cut(s) 1353
TseFI GTSAC 2 cut(s) 140, 866
TseI GCWGC 2 cut(s) 356, 1355
Tsp45I GTSAC 2 cut(s) 140, 866
TspDTI ATGAA 7 cut(s) 32, 285, 363, 600, 689, 1404, 1436
TspGWI ACGGA 1 cut(s) 511
TspRI CASTG 1 cut(s) 1353
XagI CCTNNNNNAGG 1 cut(s) 461
XapI RAATTY 3 cut(s) 645, 711, 990
XceI RCATGY 1 cut(s) 1474
XmnI GAANNNNTTC 1 cut(s) 306
XspI CTAG 5 cut(s) 332, 530, 995, 1410, 1455
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.