pycom02g22810

Type I inositol 1,4,5-trisphosphate 5-phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0003538
Physical Location & Seq
Reverse (-)
20978204 .. 20981396
3193 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g22810.1

Sequence Viewer

Length: 1407 bp
ATGACTATCAACAATCAAGACGTGAAAAAGAAGCAGTCTAACCCGTCAGTTGACATTCAATCTATAAGTGACACAGGGTCTCAAATGGCTGCCGGTCAATATGTTCAGTCCTTTCGGGTTTTTGTAGCGACATGGAATGCAGGAGGGAAATCTCCTCATAGTGGCCTTCATCTGGATGATTTTCTGCAGGTTAATAATGAATCAGACATATATGTCTTGGGTTTCCAGGAAATTGTTCCGCTAAATGCTGGAAATGTACTAGTTCTAGAAGATAATGAACCTGCAGCAAAATGGCTCGCTTTAATCAATCAGTCACTAAACAAATCACCTGACGGGTGTTCAAGAAGCAAAGCAGGTGGTTCACGATTTTCCTCGAAGCCTTCCCTTAAAAAGGTCAGTAAGACTTTTAGGACAGAGAGCAAACAGAGGTTGAAGAGTTGCAACTGCCCTTTTGAACTAGAAAGGAAACAAAGTAAAGATTTTTTCTTTCGGTGCCAACAATCAAACATAAATGAAGACGACCTTTCTTCAGATCCGGATGAGGATGGATCTAATGCCTTTGATATATCAGAAATTTCCATGGCCTCTGCTACCAACCAGATGAAGTACGGCCTTATAGCAAGTAAGCAAATGGTAGGAATTTTTGTTACTATTTGGGCGAGGAAAGAGTATATACAATATGTTAGCCACTTGAGGATCTCTTGCATCAGTCGCGGGATAATGGGACTTGGAAACAAGGGCTGTATATCTGTGAGCTTCTTATTCCATCAGACAAGCTTTTGCTTTGTCTGCAGTCACTTGGCATCTGGGGAGAAAGAGGGGGATGAGCTTAGGAGAAATCTGGATGTATTAGAGATTCTTAAAAGCACTCAGTTTCCGAAGATTTGCAAAACGGCTCATAGTAGGATGCCGGATAAAATTCTAGGACATGATGTGGTCATATGGTTAGGGGACTTGAATTACCGCATTGCTTTGAGCTACTCTGAAACCCAAAAGCTATTGATGCACAACGACTGGGATGCACTTCTTGACAAAGATCAGCTCAAGATCGAAAGAGAAGCAGGGCGAGTATTCAAGGGATGGAAAGAGGGGAAAATCTACTTTGCACCTACTTACAAATACTTTTATAACTCAGACACATATTTTGGAGAGATAAAAACATCAAAAAAGAAAAGAAGAACACCAGCTTGGTGTGATAGAATACTATGGCATGGAAATGGGATTAGACAACTTTCTTACATACGTGGAGAGTCTCAGTTCTCTGACCATCGGCCAGTGTGTGCAGCATTTTTTGTGGATGTTGTAGTTAATGAGAATGAAAATAAGAAGGGATCGCCTAGCTCTAATATGAAAATCGAATTTGAAGAGCTTTTACCAACTGCTAGATACCAGAGAAACATGTACTAA

Protein Analysis

469

Amino Acids

53.21

Weight (kDa)

8.77

Isoelectric Point (pI)

45.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos2 PF22669 198 - 428 1.1e-60 Endonuclease/Exonuclease/phosphatase family 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1128
AarI CACCTGC 1 cut(s) 344
AasI GACNNNNNNGTC 1 cut(s) 212
Acc36I ACCTGC 3 cut(s) 178, 289, 344
AccB1I GGYRCC 1 cut(s) 492
AccII CGCG 1 cut(s) 714
AccIII TCCGGA 1 cut(s) 535
AciI CCGC 3 cut(s) 239, 714, 964
AclWI GGATC 4 cut(s) 527, 556, 704, 1339
AcoI YGGCCR 1 cut(s) 1271
AcsI RAATTY 4 cut(s) 573, 639, 918, 1358
AcuI CTGAAG 1 cut(s) 513
AfaI GTAC 3 cut(s) 258, 608, 1403
AfiI CCNNNNNNNGG 3 cut(s) 161, 172, 391
AflIII ACRYGT 1 cut(s) 1398
AgsI TTSAA 7 cut(s) 59, 342, 433, 455, 958, 1075, 1364
AhdI GACNNNNNGTC 1 cut(s) 76
AhlI ACTAGT 1 cut(s) 259
AjiI CACGTC 1 cut(s) 22
AjnI CCWGG 1 cut(s) 225
AjuI GAANNNNNNNTTGG 2 cut(s) 1171, 1203
AluBI AGCT 9 cut(s) 756, 777, 829, 978, 997, 1042, 1187, 1341, 1369
AluI AGCT 9 cut(s) 756, 777, 829, 978, 997, 1042, 1187, 1341, 1369
Alw26I GTCTC 2 cut(s) 84, 1257
AlwI GGATC 4 cut(s) 527, 556, 704, 1339
Aor13HI TCCGGA 1 cut(s) 535
AoxI GGCC 4 cut(s) 163, 582, 610, 1271
ApeKI GCWGC 3 cut(s) 89, 284, 1283
ApoI RAATTY 4 cut(s) 573, 639, 918, 1358
ArsI GACNNNNNNTTYG 2 cut(s) 1127, 1159
Asp700I GAANNNNTTC 1 cut(s) 234
AsuHPI GGTGA 1 cut(s) 318
BanI GGYRCC 1 cut(s) 492
BarI GAAGNNNNNNTAC 2 cut(s) 1356, 1388
BbsI GAAGAC 1 cut(s) 522
BbvI GCAGC 3 cut(s) 76, 296, 1295
BccI CCATC 4 cut(s) 539, 774, 1074, 1275
BceAI ACGGC 2 cut(s) 625, 909
BcgI CGANNNNNNTGC 2 cut(s) 1001, 1035
BciT130I CCWGG 1 cut(s) 227
BcoDI GTCTC 2 cut(s) 84, 1257
BcuI ACTAGT 1 cut(s) 259
BfaI CTAG 6 cut(s) 260, 266, 458, 923, 1338, 1383
BfmI CTRYAG 3 cut(s) 185, 282, 790
BfuAI ACCTGC 3 cut(s) 178, 289, 344
BisI GCNGC 3 cut(s) 90, 285, 1284
BlsI GCNGC 3 cut(s) 91, 286, 1285
Bme1390I CCNGG 1 cut(s) 227
BmeRI GACNNNNNGTC 1 cut(s) 76
BmgBI CACGTC 1 cut(s) 22
BmiI GGNNCC 1 cut(s) 494
BmrFI CCNGG 1 cut(s) 227
BmrI ACTGGG 1 cut(s) 1024
BmsI GCATC 5 cut(s) 714, 812, 897, 993, 1009
BmuI ACTGGG 1 cut(s) 1024
BpiI GAAGAC 1 cut(s) 522
Bpu10I CCTNAGC 1 cut(s) 830
BpuEI CTTGAG 2 cut(s) 712, 1028
BsaAI YACGTR 1 cut(s) 1244
BsaI GGTCTC 1 cut(s) 84
BsaJI CCNNGG 1 cut(s) 579
BsaWI WCCGGW 1 cut(s) 535
Bsc4I CCNNNNNNNGG 3 cut(s) 161, 172, 391
Bse118I RCCGGY 1 cut(s) 92
Bse1I ACTGG 2 cut(s) 1019, 1274
Bse3DI GCAATG 1 cut(s) 966
BseAI TCCGGA 1 cut(s) 535
BseBI CCWGG 1 cut(s) 227
BseDI CCNNGG 1 cut(s) 579
BseGI GGATG 9 cut(s) 181, 544, 550, 829, 850, 912, 1024, 1085, 1303
BseLI CCNNNNNNNGG 3 cut(s) 161, 172, 391
BseMI GCAATG 1 cut(s) 966
BseMII CTCAG 3 cut(s) 884, 1146, 1268
BseNI ACTGG 2 cut(s) 1019, 1274
BseRI GAGGAG 1 cut(s) 144
BseXI GCAGC 3 cut(s) 76, 296, 1295
BsgI GTGCAG 1 cut(s) 1302
Bsh1236I CGCG 1 cut(s) 714
BshFI GGCC 4 cut(s) 165, 584, 612, 1273
BshNI GGYRCC 1 cut(s) 492
BsiSI CCGG 3 cut(s) 93, 536, 911
BslFI GGGAC 2 cut(s) 738, 965
BslI CCNNNNNNNGG 3 cut(s) 161, 172, 391
BsmAI GTCTC 2 cut(s) 84, 1257
BsmFI GGGAC 2 cut(s) 738, 965
BsmI GAATGC 1 cut(s) 142
BsnI GGCC 4 cut(s) 165, 584, 612, 1273
Bso31I GGTCTC 1 cut(s) 84
Bsp13I TCCGGA 1 cut(s) 535
Bsp143I GATC 6 cut(s) 532, 548, 696, 1036, 1047, 1331
Bsp19I CCATGG 1 cut(s) 579
BspACI CCGC 3 cut(s) 239, 714, 964
BspANI GGCC 4 cut(s) 165, 584, 612, 1273
BspCNI CTCAG 3 cut(s) 883, 1145, 1267
BspEI TCCGGA 1 cut(s) 535
BspFNI CGCG 1 cut(s) 714
BspLI GGNNCC 1 cut(s) 494
BspMAI CTGCAG 3 cut(s) 189, 286, 794
BspMI ACCTGC 3 cut(s) 178, 289, 344
BspPI GGATC 4 cut(s) 527, 556, 704, 1339
BspQI GCTCTTC 1 cut(s) 1359
BspT107I GGYRCC 1 cut(s) 492
BspTNI GGTCTC 1 cut(s) 84
BsrDI GCAATG 1 cut(s) 966
BsrFI RCCGGY 1 cut(s) 92
BsrI ACTGG 2 cut(s) 1019, 1274
BssAI RCCGGY 1 cut(s) 92
BssECI CCNNGG 1 cut(s) 579
BssMI GATC 6 cut(s) 532, 548, 696, 1036, 1047, 1331
BssT1I CCWWGG 1 cut(s) 579
Bst2UI CCWGG 1 cut(s) 227
Bst6I CTCTTC 2 cut(s) 428, 1359
BstBAI YACGTR 1 cut(s) 1244
BstC8I GCNNGC 1 cut(s) 297
BstDEI CTNAG 4 cut(s) 830, 870, 1132, 1254
BstDSI CCRYGG 1 cut(s) 579
BstENI CCTNNNNNAGG 1 cut(s) 389
BstF5I GGATG 9 cut(s) 181, 544, 550, 829, 850, 912, 1024, 1085, 1303
BstFNI CGCG 1 cut(s) 714
BstKTI GATC 6 cut(s) 535, 551, 699, 1039, 1050, 1334
BstMAI GTCTC 2 cut(s) 84, 1257
BstMBI GATC 6 cut(s) 532, 548, 696, 1036, 1047, 1331
BstMWI GCNNNNNNNGC 3 cut(s) 711, 789, 1003
BstNI CCWGG 1 cut(s) 227
BstNSI RCATGY 1 cut(s) 1402
BstSCI CCNGG 1 cut(s) 225
BstSFI CTRYAG 3 cut(s) 185, 282, 790
BstUI CGCG 1 cut(s) 714
BstV1I GCAGC 3 cut(s) 76, 296, 1295
BstV2I GAAGAC 1 cut(s) 522
BstX2I RGATCY 3 cut(s) 532, 548, 696
BstYI RGATCY 3 cut(s) 532, 548, 696
BsuRI GGCC 4 cut(s) 165, 584, 612, 1273
BtgI CCRYGG 1 cut(s) 579
BtrI CACGTC 1 cut(s) 22
BtsCI GGATG 9 cut(s) 181, 544, 550, 829, 850, 912, 1024, 1085, 1303
BtsIMutI CAGTG 1 cut(s) 1281
BveI ACCTGC 3 cut(s) 178, 289, 344
Cac8I GCNNGC 1 cut(s) 297
Cfr10I RCCGGY 1 cut(s) 92
Csp6I GTAC 3 cut(s) 257, 607, 1402
CspCI CAANNNNNGTGG 2 cut(s) 337, 372
CviAII CATG 5 cut(s) 132, 580, 929, 1211, 1399
CviQI GTAC 3 cut(s) 257, 607, 1402
DdeI CTNAG 4 cut(s) 830, 870, 1132, 1254
DpnI GATC 6 cut(s) 534, 550, 698, 1038, 1049, 1333
DpnII GATC 6 cut(s) 532, 548, 696, 1036, 1047, 1331
DrdI GACNNNNNNGTC 1 cut(s) 212
DriI GACNNNNNGTC 1 cut(s) 76
DseDI GACNNNNNNGTC 1 cut(s) 212
EaeI YGGCCR 1 cut(s) 1271
Eam1104I CTCTTC 2 cut(s) 428, 1359
Eam1105I GACNNNNNGTC 1 cut(s) 76
EarI CTCTTC 2 cut(s) 428, 1359
Eco130I CCWWGG 1 cut(s) 579
Eco31I GGTCTC 1 cut(s) 84
Eco57I CTGAAG 1 cut(s) 513
EcoNI CCTNNNNNAGG 1 cut(s) 389
EcoRII CCWGG 1 cut(s) 225
EcoT14I CCWWGG 1 cut(s) 579
ErhI CCWWGG 1 cut(s) 579
FaeI CATG 5 cut(s) 135, 583, 932, 1214, 1402
FalI AAGNNNNNCTT 2 cut(s) 507, 539
FaqI GGGAC 2 cut(s) 738, 965
FatI CATG 5 cut(s) 131, 579, 928, 1210, 1398
FauI CCCGC 1 cut(s) 707
FauNDI CATATG 1 cut(s) 941
Fnu4HI GCNGC 3 cut(s) 90, 285, 1284
FokI GGATG 9 cut(s) 188, 551, 557, 836, 857, 919, 1031, 1092, 1310
Fsp4HI GCNGC 3 cut(s) 90, 285, 1284
FspBI CTAG 6 cut(s) 260, 266, 458, 923, 1338, 1383
GluI GCNGC 3 cut(s) 90, 285, 1284
HaeIII GGCC 4 cut(s) 165, 584, 612, 1273
HapII CCGG 3 cut(s) 93, 536, 911
Hin1II CATG 5 cut(s) 135, 583, 932, 1214, 1402
HincII GTYRAC 1 cut(s) 52
HindII GTYRAC 1 cut(s) 52
HindIII AAGCTT 1 cut(s) 775
HinfI GANTC 3 cut(s) 200, 856, 1250
HpaII CCGG 3 cut(s) 93, 536, 911
HphI GGTGA 1 cut(s) 318
Hpy166II GTNNAC 2 cut(s) 52, 362
Hpy188I TCNGA 8 cut(s) 205, 532, 571, 771, 879, 985, 1135, 1264
Hpy188III TCNNGA 9 cut(s) 17, 173, 266, 342, 363, 536, 842, 1028, 1045
Hpy8I GTNNAC 2 cut(s) 52, 362
HpyAV CCTTC 3 cut(s) 176, 390, 1321
HpyCH4IV ACGT 2 cut(s) 21, 1243
HpyF10VI GCNNNNNNNGC 3 cut(s) 711, 789, 1003
HpyF3I CTNAG 4 cut(s) 830, 870, 1132, 1254
HpySE526I ACGT 2 cut(s) 21, 1243
Hsp92II CATG 5 cut(s) 135, 583, 932, 1214, 1402
Kpn2I TCCGGA 1 cut(s) 535
Kzo9I GATC 6 cut(s) 532, 548, 696, 1036, 1047, 1331
LguI GCTCTTC 1 cut(s) 1359
Lsp1109I GCAGC 3 cut(s) 76, 296, 1295
LweI GCATC 5 cut(s) 714, 812, 897, 993, 1009
MaeI CTAG 6 cut(s) 260, 266, 458, 923, 1338, 1383
MaeII ACGT 2 cut(s) 21, 1243
MaeIII GTNAC 4 cut(s) 68, 312, 646, 794
MalI GATC 6 cut(s) 534, 550, 698, 1038, 1049, 1333
MboI GATC 6 cut(s) 532, 548, 696, 1036, 1047, 1331
MboII GAAGA 7 cut(s) 281, 445, 519, 527, 892, 1188, 1376
MflI RGATCY 3 cut(s) 532, 548, 696
MluCI AATT 6 cut(s) 231, 573, 639, 918, 958, 1358
MlyI GAGTC 1 cut(s) 1259
MroI TCCGGA 1 cut(s) 535
MroXI GAANNNNTTC 1 cut(s) 234
MseI TTAA 5 cut(s) 192, 302, 387, 861, 1308
MslI CAYNNNNRTG 2 cut(s) 174, 1215
MspI CCGG 3 cut(s) 93, 536, 911
MspR9I CCNGG 1 cut(s) 227
Mva1269I GAATGC 1 cut(s) 142
MvaI CCWGG 1 cut(s) 227
MvnI CGCG 1 cut(s) 714
MwoI GCNNNNNNNGC 3 cut(s) 711, 789, 1003
NcoI CCATGG 1 cut(s) 579
NdeI CATATG 1 cut(s) 941
NdeII GATC 6 cut(s) 532, 548, 696, 1036, 1047, 1331
NlaIII CATG 5 cut(s) 135, 583, 932, 1214, 1402
NlaIV GGNNCC 1 cut(s) 494
NmuCI GTSAC 3 cut(s) 68, 312, 794
NspI RCATGY 1 cut(s) 1402
PaqCI CACCTGC 1 cut(s) 344
PciI ACATGT 1 cut(s) 1398
PciSI GCTCTTC 1 cut(s) 1359
PctI GAATGC 1 cut(s) 142
PdmI GAANNNNTTC 1 cut(s) 234
PfeI GAWTC 2 cut(s) 200, 856
PfoI TCCNGGA 1 cut(s) 225
PkrI GCNGC 3 cut(s) 91, 286, 1285
PleI GAGTC 1 cut(s) 1258
PpsI GAGTC 1 cut(s) 1258
Ppu21I YACGTR 1 cut(s) 1244
PscI ACATGT 1 cut(s) 1398
PsiI TTATAA 1 cut(s) 1128
Psp6I CCWGG 1 cut(s) 225
PspGI CCWGG 1 cut(s) 225
PspN4I GGNNCC 1 cut(s) 494
PstI CTGCAG 3 cut(s) 189, 286, 794
PsuI RGATCY 3 cut(s) 532, 548, 696
RsaI GTAC 3 cut(s) 258, 608, 1403
RsaNI GTAC 3 cut(s) 257, 607, 1402
RseI CAYNNNNRTG 2 cut(s) 174, 1215
SapI GCTCTTC 1 cut(s) 1359
SaqAI TTAA 5 cut(s) 192, 302, 387, 861, 1308
SatI GCNGC 3 cut(s) 90, 285, 1284
Sau3AI GATC 6 cut(s) 532, 548, 696, 1036, 1047, 1331
SchI GAGTC 1 cut(s) 1259
ScrFI CCNGG 1 cut(s) 227
SfaNI GCATC 5 cut(s) 714, 812, 897, 993, 1009
SfcI CTRYAG 3 cut(s) 185, 282, 790
SmiMI CAYNNNNRTG 2 cut(s) 174, 1215
SmlI CTYRAG 2 cut(s) 691, 1043
SmoI CTYRAG 2 cut(s) 691, 1043
SpeI ACTAGT 1 cut(s) 259
Sse9I AATT 6 cut(s) 231, 573, 639, 918, 958, 1358
SsiI CCGC 3 cut(s) 239, 714, 964
SspMI CTAG 6 cut(s) 260, 266, 458, 923, 1338, 1383
StyD4I CCNGG 1 cut(s) 225
StyI CCWWGG 1 cut(s) 579
TaiI ACGT 2 cut(s) 24, 1246
TaqI TCGA 3 cut(s) 374, 1050, 1356
TasI AATT 6 cut(s) 231, 573, 639, 918, 958, 1358
TatI WGTACW 2 cut(s) 256, 1401
TfiI GAWTC 2 cut(s) 200, 856
Tru1I TTAA 5 cut(s) 192, 302, 387, 861, 1308
Tru9I TTAA 5 cut(s) 192, 302, 387, 861, 1308
TscAI CASTG 1 cut(s) 1281
TseFI GTSAC 3 cut(s) 68, 312, 794
TseI GCWGC 3 cut(s) 89, 284, 1283
Tsp45I GTSAC 3 cut(s) 68, 312, 794
TspDTI ATGAA 7 cut(s) 158, 213, 291, 528, 617, 1332, 1364
TspRI CASTG 1 cut(s) 1281
XagI CCTNNNNNAGG 1 cut(s) 389
XapI RAATTY 4 cut(s) 573, 639, 918, 1358
XbaI TCTAGA 1 cut(s) 265
XceI RCATGY 1 cut(s) 1402
XmnI GAANNNNTTC 1 cut(s) 234
XspI CTAG 6 cut(s) 260, 266, 458, 923, 1338, 1383
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.