Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0003046

Physical Location & Seq

Genomic Coordinates Reverse (-)

1588461 .. 1591960

Gene Structure 3500 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1020600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1284 bp

ATGGCCGCAGCAGCTATGGAATTAGCTCTTATAAATCCGTGGACTTTATTCCATCCCAAGTCTCCAAACCCATCAAAGCTCAGAGCTTTCACCATCCGATGCTCCTCCGCCACCTCAACCACCGTCCCAGATTTGGCAGAAAGACCTTGGAAGACCTCAGATGCTAGGCTTGTGCTTGAAGATGGTTCAATCTGGAGAGCGAAGTCGTTTGGCGCTTCAGGGACCCAAGTTGGTGAAGTTGTATTCAATACATCCTTGACTGGGTACCAAGAAATTATTACAGATCCTAGTTATGCTGGCCAATTTGTCCTGATGACAAACCCACATATTGGAAATACTGGTGTAAATTTTGATGATGAAGAATCAAGGAAATGCTTCCTTGCCGGTCTGGTGATCAGAAGTTTAAGCATCAGTACTTCAAATTGGAGATGTGCAGAAACAATTGGTGATTATTTAGCAGAAAGGAACATCATGGGAATTTATGATGTTGACACACGTGCTATCACCCGAAGATTAAGGCAAGATGGAAGCCTTATTGGTGTTTTAAGCACGGAAAAGACAAAATCAGATGAAGAACTATTGGAAATGTCTCGTTCGTGGGACATTGTTGGGGTCGATCTAATAAGTGGTGTGTCATGTGAGGCCCCTCATGATTGGGTTGATAAAACAGATTCAGAGTGGGCATTTAACTCCAGTAAAAGAGATGGAGATGCATTTCATGTGGTTGCATATGATTTTGGAATCAAGCATAATATACTCAGGCGCTTGGCTTCGTATGGTTGTAAAATTACTGTTGTGCCTTCAACATGGCCAGCATCAGAGACTCTTAAAATGAAACCAGATGGGGTACTTTTCAGCAATGGCCCCGGAGACCCATCTGCTGTTCCTTATGCTGTTGAAACAGTCAAGGAGATTCTTGGGAAGGTTCCAGTTTTTGGAATTTGCATGGGCCATCAGTTGCTTGGCCTGGCATTAGGCGGTAAAACTTTCAAAATGAAGTTTGGCCACCATGGAGGAAATCATCCAGTCCGTAATCTTCATACTGGACATGTTGAGATAAGTGCTCAGAACCACAACTTTGCAGTTGAGGCTGGATCACTTCCGGAGGGTGTAGAAGTAACTCATGTCAATCTCAATGATGGAAGTTGTGCTGGCCTTGCCTATCCTGCACGAAACATCATGTCTATTCAGTACCACCCTGAAGCATCCCCAGGACCTCATGATTCGGACTATGTTTTTAGCGAATTCATAGAGCTGATGAAGAAAGTAAAAGTAAATGCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000050 GO:0003674 GO:0003824 GO:0004088 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005951 GO:0006082 GO:0006520 GO:0006525 GO:0006526 GO:0006807 GO:0006950 GO:0007154 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009064 GO:0009084 GO:0009267 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009987 GO:0009991 GO:0016036 GO:0016053 GO:0016874 GO:0016879 GO:0016884 GO:0019627 GO:0019752 GO:0031667 GO:0031668 GO:0031669 GO:0032991 GO:0033554 GO:0034641 GO:0042594 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0050896 GO:0051716 GO:0071496 GO:0071704 GO:0071941 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607 GO:1902494

KEGG Pathways

Metabolic & Signaling

ko00240 ko00250 ko01100 map00240 map00250 map01100

Pfam Domains

Protein Families

CPSase_sm_chain GATase

Protein Analysis

428

Amino Acids

46.64

Weight (kDa)

5.93

Isoelectric Point (pI)

40.92

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CPSase_sm_chain	PF00988	57 - 181	1.6e-48	Carbamoyl-phosphate synthase small chain, CPSase domain
GATase	PF00117	243 - 418	8.9e-55	Glutamine amidotransferase class-I

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003046)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G27740
fragaria_vesca	FvH4_3g43670 FvH4_3g43670 FvH4_5g29030 FvH4_5g29030 FvH4_5g29030
malus_domestica	MD03G1020600.v1.1 MD11G1021800.v1.1 MD15G1342500.v1.1
prunus_persica	Prupe.6G016000_v2.0.a1 Prupe.6G016000_v2.0.a1 Prupe.6G163300_v2.0.a1
pyrus_communis	pycom03g01750 pycom11g01770
rosa_chinensis	RchiOBHm_Chr2g0150151 RchiOBHm_Chr5g0078331
rosa_laevigata	RLG00000020471 RLG00000036765 RLG00000036767
rosa_multiflora	Rmu_sc0000631.1_g000032 Rmu_sc0009445.1_g000001 Rmu_ssc0000020.1_g000013
rosa_roxburghii	Rroxscaffold_1G00003910 Rroxscaffold_1G00003930 Rroxscaffold_2G00097400
rosa_rugosa	Rorug02G0419400 Rorug05G0460000
rosa_samantha	Rh2AG478200 Rh2CG464400 Rh2DG499600 Rh5AG513900 Rh5AG514000 Rh5BG536900 Rh5DG548500
rosa_wichuraiana	Rw2G039040 Rw5G047700 Rw5G047720 Rw5G047860

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	32
Acc65I	GGTACC	1 cut(s)	264
AccB1I	GGYRCC	1 cut(s)	264
AccB7I	CCANNNNNTGG	2 cut(s)	329, 935
AccIII	TCCGGA	1 cut(s)	1102
AciI	CCGC	3 cut(s)	6, 108, 978
AclWI	GGATC	2 cut(s)	278, 1102
AcoI	YGGCCR	4 cut(s)	3, 298, 809, 1003
AcsI	RAATTY	4 cut(s)	346, 477, 939, 1244
AcuI	CTGAAG	2 cut(s)	201, 1221
AcvI	CACGTG	1 cut(s)	497
AfaI	GTAC	4 cut(s)	266, 415, 849, 1193
AfiI	CCNNNNNNNGG	4 cut(s)	133, 261, 329, 935
AflIII	ACRYGT	2 cut(s)	494, 1048
AgsI	TTSAA	7 cut(s)	179, 189, 247, 420, 804, 899, 991
AjnI	CCWGG	2 cut(s)	966, 1210
AluBI	AGCT	5 cut(s)	14, 26, 79, 86, 1255
AluI	AGCT	5 cut(s)	14, 26, 79, 86, 1255
Alw21I	GWGCWC	1 cut(s)	1066
Alw26I	GTCTC	4 cut(s)	66, 594, 815, 864
AlwI	GGATC	2 cut(s)	278, 1102
Aor13HI	TCCGGA	1 cut(s)	1102
AoxI	GGCC	9 cut(s)	3, 298, 642, 809, 862, 949, 964, 1003, 1153
ApeKI	GCWGC	2 cut(s)	8, 11
ApoI	RAATTY	4 cut(s)	346, 477, 939, 1244
Asp700I	GAANNNNTTC	1 cut(s)	374
Asp718I	GGTACC	1 cut(s)	264
AspLEI	GCGC	2 cut(s)	215, 765
AspS9I	GGNCC	5 cut(s)	222, 643, 863, 949, 1214
AsuC2I	CCSGG	1 cut(s)	867
AsuHPI	GGTGA	5 cut(s)	82, 245, 403, 458, 496
AvaII	GGWCC	2 cut(s)	222, 1214
BalI	TGGCCA	3 cut(s)	300, 811, 1005
BanI	GGYRCC	1 cut(s)	264
BbrPI	CACGTG	1 cut(s)	497
BbsI	GAAGAC	1 cut(s)	158
Bbv12I	GWGCWC	1 cut(s)	1066
BbvI	GCAGC	2 cut(s)	20, 23
BciT130I	CCWGG	2 cut(s)	968, 1212
BclI	TGATCA	1 cut(s)	393
BcnI	CCSGG	1 cut(s)	867
BcoDI	GTCTC	4 cut(s)	66, 594, 815, 864
BfaI	CTAG	2 cut(s)	165, 288
BfoI	RGCGCY	2 cut(s)	216, 766
BisI	GCNGC	3 cut(s)	6, 9, 12
BlsI	GCNGC	3 cut(s)	7, 10, 13
BmcAI	AGTACT	1 cut(s)	415
Bme1390I	CCNGG	3 cut(s)	867, 968, 1212
Bme18I	GGWCC	2 cut(s)	222, 1214
BmgT120I	GGNCC	5 cut(s)	222, 643, 863, 949, 1214
BmiI	GGNNCC	6 cut(s)	223, 224, 266, 645, 865, 927
BmrFI	CCNGG	3 cut(s)	867, 968, 1212
BmrI	ACTGGG	1 cut(s)	270
BmsI	GCATC	6 cut(s)	89, 151, 417, 700, 824, 1214
BmuI	ACTGGG	1 cut(s)	270
BpiI	GAAGAC	1 cut(s)	158
BpmI	CTGGAG	2 cut(s)	214, 676
BpuMI	CCSGG	1 cut(s)	867
BsaAI	YACGTR	1 cut(s)	497
BsaI	GGTCTC	1 cut(s)	864
BsaJI	CCNNGG	5 cut(s)	38, 146, 865, 1009, 1210
BsaWI	WCCGGW	1 cut(s)	1102
Bsc4I	CCNNNNNNNGG	4 cut(s)	133, 261, 329, 935
Bse118I	RCCGGY	1 cut(s)	383
Bse1I	ACTGG	6 cut(s)	265, 343, 693, 929, 1025, 1048
Bse3DI	GCAATG	1 cut(s)	865
BseAI	TCCGGA	1 cut(s)	1102
BseBI	CCWGG	2 cut(s)	968, 1212
BseDI	CCNNGG	5 cut(s)	38, 146, 865, 1009, 1210
BseGI	GGATG	5 cut(s)	52, 93, 251, 1021, 1205
BseLI	CCNNNNNNNGG	4 cut(s)	133, 261, 329, 935
BseMI	GCAATG	1 cut(s)	865
BseMII	CTCAG	4 cut(s)	94, 171, 772, 1079
BseNI	ACTGG	6 cut(s)	265, 343, 693, 929, 1025, 1048
BseRI	GAGGAG	1 cut(s)	94
BseXI	GCAGC	2 cut(s)	20, 23
BsgI	GTGCAG	2 cut(s)	453, 1152
BshFI	GGCC	9 cut(s)	5, 300, 644, 811, 864, 951, 966, 1005, 1155
BshNI	GGYRCC	1 cut(s)	264
BsiHKAI	GWGCWC	1 cut(s)	1066
BsiSI	CCGG	3 cut(s)	384, 867, 1103
BslFI	GGGAC	3 cut(s)	110, 235, 614
BslI	CCNNNNNNNGG	4 cut(s)	133, 261, 329, 935
BsmAI	GTCTC	4 cut(s)	66, 594, 815, 864
BsmFI	GGGAC	3 cut(s)	110, 235, 614
BsnI	GGCC	9 cut(s)	5, 300, 644, 811, 864, 951, 966, 1005, 1155
Bso31I	GGTCTC	1 cut(s)	864
Bsp1286I	GDGCHC	1 cut(s)	1066
Bsp13I	TCCGGA	1 cut(s)	1102
Bsp143I	GATC	4 cut(s)	283, 393, 616, 1094
Bsp19I	CCATGG	1 cut(s)	1009
BspACI	CCGC	3 cut(s)	6, 108, 978
BspANI	GGCC	9 cut(s)	5, 300, 644, 811, 864, 951, 966, 1005, 1155
BspCNI	CTCAG	4 cut(s)	93, 170, 771, 1078
BspEI	TCCGGA	1 cut(s)	1102
BspHI	TCATGA	2 cut(s)	649, 1219
BspLI	GGNNCC	6 cut(s)	223, 224, 266, 645, 865, 927
BspPI	GGATC	2 cut(s)	278, 1102
BspT107I	GGYRCC	1 cut(s)	264
BspTNI	GGTCTC	1 cut(s)	864
BsrDI	GCAATG	1 cut(s)	865
BsrFI	RCCGGY	1 cut(s)	383
BsrI	ACTGG	6 cut(s)	265, 343, 693, 929, 1025, 1048
BssAI	RCCGGY	1 cut(s)	383
BssECI	CCNNGG	5 cut(s)	38, 146, 865, 1009, 1210
BssMI	GATC	4 cut(s)	283, 393, 616, 1094
BssT1I	CCWWGG	2 cut(s)	146, 1009
Bst2UI	CCWGG	2 cut(s)	968, 1212
Bst4CI	ACNGT	3 cut(s)	124, 793, 904
BstBAI	YACGTR	1 cut(s)	497
BstC8I	GCNNGC	3 cut(s)	298, 813, 1153
BstDEI	CTNAG	4 cut(s)	80, 157, 758, 1065
BstDSI	CCRYGG	2 cut(s)	38, 1009
BstF5I	GGATG	5 cut(s)	52, 93, 251, 1021, 1205
BstH2I	RGCGCY	2 cut(s)	216, 766
BstHHI	GCGC	2 cut(s)	215, 765
BstKTI	GATC	4 cut(s)	286, 396, 619, 1097
BstMAI	GTCTC	4 cut(s)	66, 594, 815, 864
BstMBI	GATC	4 cut(s)	283, 393, 616, 1094
BstMWI	GCNNNNNNNGC	4 cut(s)	11, 1088, 1157, 1166
BstNI	CCWGG	2 cut(s)	968, 1212
BstNSI	RCATGY	1 cut(s)	1052
BstSCI	CCNGG	3 cut(s)	865, 966, 1210
BstV1I	GCAGC	2 cut(s)	20, 23
BstV2I	GAAGAC	1 cut(s)	158
BstX2I	RGATCY	1 cut(s)	283
BstYI	RGATCY	1 cut(s)	283
BsuRI	GGCC	9 cut(s)	5, 300, 644, 811, 864, 951, 966, 1005, 1155
BtgI	CCRYGG	2 cut(s)	38, 1009
BtsCI	GGATG	5 cut(s)	52, 93, 251, 1021, 1205
Cac8I	GCNNGC	3 cut(s)	298, 813, 1153
CciI	TCATGA	2 cut(s)	649, 1219
CfoI	GCGC	2 cut(s)	215, 765
Cfr10I	RCCGGY	1 cut(s)	383
Cfr13I	GGNCC	5 cut(s)	222, 643, 863, 949, 1214
Csp6I	GTAC	4 cut(s)	265, 414, 848, 1192
CviQI	GTAC	4 cut(s)	265, 414, 848, 1192
DdeI	CTNAG	4 cut(s)	80, 157, 758, 1065
DpnI	GATC	4 cut(s)	285, 395, 618, 1096
DpnII	GATC	4 cut(s)	283, 393, 616, 1094
EaeI	YGGCCR	4 cut(s)	3, 298, 809, 1003
EciI	GGCGGA	1 cut(s)	97
Eco130I	CCWWGG	2 cut(s)	146, 1009
Eco31I	GGTCTC	1 cut(s)	864
Eco47I	GGWCC	2 cut(s)	222, 1214
Eco57I	CTGAAG	2 cut(s)	201, 1221
Eco72I	CACGTG	1 cut(s)	497
EcoO109I	RGGNCCY	3 cut(s)	222, 643, 1214
EcoRI	GAATTC	1 cut(s)	1244
EcoRII	CCWGG	2 cut(s)	966, 1210
EcoT14I	CCWWGG	2 cut(s)	146, 1009
EcoT22I	ATGCAT	2 cut(s)	715, 1282
ErhI	CCWWGG	2 cut(s)	146, 1009
FaqI	GGGAC	3 cut(s)	110, 235, 614
FauNDI	CATATG	1 cut(s)	730
FbaI	TGATCA	1 cut(s)	393
Fnu4HI	GCNGC	3 cut(s)	6, 9, 12
FokI	GGATG	5 cut(s)	39, 80, 238, 1008, 1192
Fsp4HI	GCNGC	3 cut(s)	6, 9, 12
FspBI	CTAG	2 cut(s)	165, 288
GlaI	GCGC	2 cut(s)	214, 764
GluI	GCNGC	3 cut(s)	6, 9, 12
GsuI	CTGGAG	2 cut(s)	214, 676
HaeII	RGCGCY	2 cut(s)	216, 766
HaeIII	GGCC	9 cut(s)	5, 300, 644, 811, 864, 951, 966, 1005, 1155
HapII	CCGG	3 cut(s)	384, 867, 1103
HhaI	GCGC	2 cut(s)	215, 765
Hin6I	GCGC	2 cut(s)	213, 763
HinP1I	GCGC	2 cut(s)	213, 763
HincII	GTYRAC	1 cut(s)	490
HindII	GTYRAC	1 cut(s)	490
HinfI	GANTC	6 cut(s)	362, 671, 741, 823, 913, 1223
HpaII	CCGG	3 cut(s)	384, 867, 1103
HphI	GGTGA	5 cut(s)	82, 245, 403, 458, 496
Hpy166II	GTNNAC	2 cut(s)	42, 490
Hpy188I	TCNGA	9 cut(s)	83, 98, 160, 398, 568, 676, 820, 1068, 1228
Hpy188III	TCNNGA	5 cut(s)	193, 310, 650, 1103, 1220
Hpy8I	GTNNAC	2 cut(s)	42, 490
HpyAV	CCTTC	2 cut(s)	810, 916
HpyCH4III	ACNGT	3 cut(s)	124, 793, 904
HpyCH4IV	ACGT	1 cut(s)	496
HpyCH4V	TGCA	7 cut(s)	434, 713, 728, 945, 1082, 1169, 1280
HpyF10VI	GCNNNNNNNGC	4 cut(s)	11, 1088, 1157, 1166
HpyF3I	CTNAG	4 cut(s)	80, 157, 758, 1065
HpySE526I	ACGT	1 cut(s)	496
HspAI	GCGC	2 cut(s)	213, 763
KflI	GGGWCCC	1 cut(s)	222
Kpn2I	TCCGGA	1 cut(s)	1102
KpnI	GGTACC	1 cut(s)	268
Ksp22I	TGATCA	1 cut(s)	393
Kzo9I	GATC	4 cut(s)	283, 393, 616, 1094
LmnI	GCTCC	1 cut(s)	107
Lsp1109I	GCAGC	2 cut(s)	20, 23
LweI	GCATC	6 cut(s)	89, 151, 417, 700, 824, 1214
MaeI	CTAG	2 cut(s)	165, 288
MaeII	ACGT	1 cut(s)	496
MaeIII	GTNAC	1 cut(s)	1117
MalI	GATC	4 cut(s)	285, 395, 618, 1096
MboI	GATC	4 cut(s)	283, 393, 616, 1094
MboII	GAAGA	7 cut(s)	163, 191, 371, 522, 584, 1028, 1273
MfeI	CAATTG	1 cut(s)	441
MflI	RGATCY	1 cut(s)	283
MhlI	GDGCHC	1 cut(s)	1066
MlsI	TGGCCA	3 cut(s)	300, 811, 1005
MluNI	TGGCCA	3 cut(s)	300, 811, 1005
MlyI	GAGTC	1 cut(s)	817
MnlI	CCTC	9 cut(s)	115, 124, 166, 634, 657, 1007, 1081, 1099, 1227
Mox20I	TGGCCA	3 cut(s)	300, 811, 1005
Mph1103I	ATGCAT	2 cut(s)	715, 1282
MroI	TCCGGA	1 cut(s)	1102
MroXI	GAANNNNTTC	1 cut(s)	374
MscI	TGGCCA	3 cut(s)	300, 811, 1005
MseI	TTAA	5 cut(s)	404, 515, 545, 687, 828
Msp20I	TGGCCA	3 cut(s)	300, 811, 1005
MspI	CCGG	3 cut(s)	384, 867, 1103
MspR9I	CCNGG	3 cut(s)	867, 968, 1212
MunI	CAATTG	1 cut(s)	441
MvaI	CCWGG	2 cut(s)	968, 1212
MwoI	GCNNNNNNNGC	4 cut(s)	11, 1088, 1157, 1166
NciI	CCSGG	1 cut(s)	867
NcoI	CCATGG	1 cut(s)	1009
NdeI	CATATG	1 cut(s)	730
NdeII	GATC	4 cut(s)	283, 393, 616, 1094
NlaIV	GGNNCC	6 cut(s)	223, 224, 266, 645, 865, 927
NsiI	ATGCAT	2 cut(s)	715, 1282
NspI	RCATGY	1 cut(s)	1052
PagI	TCATGA	2 cut(s)	649, 1219
PciI	ACATGT	1 cut(s)	1048
PdmI	GAANNNNTTC	1 cut(s)	374
PfeI	GAWTC	5 cut(s)	362, 671, 741, 913, 1223
PflMI	CCANNNNNTGG	2 cut(s)	329, 935
PkrI	GCNGC	3 cut(s)	7, 10, 13
PleI	GAGTC	1 cut(s)	817
PmaCI	CACGTG	1 cut(s)	497
PmlI	CACGTG	1 cut(s)	497
PpsI	GAGTC	1 cut(s)	817
Ppu21I	YACGTR	1 cut(s)	497
PpuMI	RGGWCCY	2 cut(s)	222, 1214
PscI	ACATGT	1 cut(s)	1048
PsiI	TTATAA	1 cut(s)	32
Psp5II	RGGWCCY	2 cut(s)	222, 1214
Psp6I	CCWGG	2 cut(s)	966, 1210
PspCI	CACGTG	1 cut(s)	497
PspGI	CCWGG	2 cut(s)	966, 1210
PspN4I	GGNNCC	6 cut(s)	223, 224, 266, 645, 865, 927
PspPI	GGNCC	5 cut(s)	222, 643, 863, 949, 1214
PspPPI	RGGWCCY	2 cut(s)	222, 1214
PsuI	RGATCY	1 cut(s)	283
RsaI	GTAC	4 cut(s)	266, 415, 849, 1193
RsaNI	GTAC	4 cut(s)	265, 414, 848, 1192
SaqAI	TTAA	5 cut(s)	404, 515, 545, 687, 828
SatI	GCNGC	3 cut(s)	6, 9, 12
Sau3AI	GATC	4 cut(s)	283, 393, 616, 1094
Sau96I	GGNCC	5 cut(s)	222, 643, 863, 949, 1214
ScaI	AGTACT	1 cut(s)	415
SchI	GAGTC	1 cut(s)	817
ScrFI	CCNGG	3 cut(s)	867, 968, 1212
SduI	GDGCHC	1 cut(s)	1066
SfaNI	GCATC	6 cut(s)	89, 151, 417, 700, 824, 1214
SinI	GGWCC	2 cut(s)	222, 1214
SsiI	CCGC	3 cut(s)	6, 108, 978
SspMI	CTAG	2 cut(s)	165, 288
StyD4I	CCNGG	3 cut(s)	865, 966, 1210
StyI	CCWWGG	2 cut(s)	146, 1009
TaaI	ACNGT	3 cut(s)	124, 793, 904
TaiI	ACGT	1 cut(s)	499
TaqI	TCGA	1 cut(s)	615
TatI	WGTACW	1 cut(s)	413
TauI	GCSGC	1 cut(s)	8
TfiI	GAWTC	5 cut(s)	362, 671, 741, 913, 1223
Tru1I	TTAA	5 cut(s)	404, 515, 545, 687, 828
Tru9I	TTAA	5 cut(s)	404, 515, 545, 687, 828
TseI	GCWGC	2 cut(s)	8, 11
TspDTI	ATGAA	8 cut(s)	372, 585, 707, 848, 1010, 1028, 1237, 1274
TspGWI	ACGGA	3 cut(s)	27, 566, 1019
Van91I	CCANNNNNTGG	2 cut(s)	329, 935
VpaK11BI	GGWCC	2 cut(s)	222, 1214
XapI	RAATTY	4 cut(s)	346, 477, 939, 1244
XceI	RCATGY	1 cut(s)	1052
XcmI	CCANNNNNNNNNTGG	1 cut(s)	959
XmnI	GAANNNNTTC	1 cut(s)	374
XspI	CTAG	2 cut(s)	165, 288
ZrmI	AGTACT	1 cut(s)	415
Zsp2I	ATGCAT	2 cut(s)	715, 1282

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.