Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0003046

Physical Location & Seq

Genomic Coordinates Reverse (-)

39612129 .. 39616166

Gene Structure 4038 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1342500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 735 bp

ATGGCCACAACCGCTGTCAACTTAGCTCTAATAAACCCGTTGACTTTGTCTGTTCCAAAGTCTCCAAATCCTTCAAATCTTAGGGTTTTCACTCTCAGACGCTCCTCCGCCGCATCAAACTCTGTCCCAGGTTTGGCAGAAACACCATGGAAGACTTCGGATGCTAGACTTGTGCTTGAAGATGGTTCAATCTGGAGGGCTAAGTCATTCGGTGCTTCAGGAACCCAAGTTGGTGAAGTTGTTTTCAATACATCCTTGACCGGGTATCAAGAAAATATTACAGACCCTAGTTATGCTGGCCAGTTTGTGCTGATGACAAACCCACATATAGGAAATACTGGTGTAAATTTTGATGACGAAGAATCAAAGCAATGCTTCCTTGGTGGTCTAGTCATTAGAAGTTTAAGTATCAGTACTTCAAATTGGAGATGTGCAGAAACATTTGGTGATTATTTAGCGGAGAGGAATATCATGGGAGTTTATGATGTTGACACGCGTGCTATCACTTGCAGATTAAGGCAAGATGGAAGCCTTAGGGTTGATTTGATAAGTGGTGTCACATGCAAGGCCCCTTATGAATGGGTTGATAAAACAAATTCCGAGTGGGAATTTAACTACAAAGGAAGAGATGGAGAGGCTTTTCATGTTATTGCATATGATTTTGGAATCAAACATAACATACTTAGGCGCTTGGCTTCCCACGGCTGTAAAATTACTGTTGTGCCATCAACATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000050 GO:0003674 GO:0003824 GO:0004088 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005951 GO:0006082 GO:0006520 GO:0006525 GO:0006526 GO:0006807 GO:0006950 GO:0007154 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009064 GO:0009084 GO:0009267 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009987 GO:0009991 GO:0016036 GO:0016053 GO:0016874 GO:0016879 GO:0016884 GO:0019627 GO:0019752 GO:0031667 GO:0031668 GO:0031669 GO:0032991 GO:0033554 GO:0034641 GO:0042594 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0050896 GO:0051716 GO:0071496 GO:0071704 GO:0071941 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607 GO:1902494

KEGG Pathways

Metabolic & Signaling

ko00240 ko00250 ko01100 map00240 map00250 map01100

Pfam Domains

Protein Families

CPSase_sm_chain GATase

Protein Analysis

245

Amino Acids

26.82

Weight (kDa)

6.43

Isoelectric Point (pI)

36.43

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CPSase_sm_chain	PF00988	57 - 179	1.5e-46	Carbamoyl-phosphate synthase small chain, CPSase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Publication-ready

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Orthologous Genes (Group: OG0003046)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G27740
fragaria_vesca	FvH4_3g43670 FvH4_3g43670 FvH4_5g29030 FvH4_5g29030 FvH4_5g29030
malus_domestica	MD03G1020600.v1.1 MD11G1021800.v1.1 MD15G1342500.v1.1
prunus_persica	Prupe.6G016000_v2.0.a1 Prupe.6G016000_v2.0.a1 Prupe.6G163300_v2.0.a1
pyrus_communis	pycom03g01750 pycom11g01770
rosa_chinensis	RchiOBHm_Chr2g0150151 RchiOBHm_Chr5g0078331
rosa_laevigata	RLG00000020471 RLG00000036765 RLG00000036767
rosa_multiflora	Rmu_sc0000631.1_g000032 Rmu_sc0009445.1_g000001 Rmu_ssc0000020.1_g000013
rosa_roxburghii	Rroxscaffold_1G00003910 Rroxscaffold_1G00003930 Rroxscaffold_2G00097400
rosa_rugosa	Rorug02G0419400 Rorug05G0460000
rosa_samantha	Rh2AG478200 Rh2CG464400 Rh2DG499600 Rh5AG513900 Rh5AG514000 Rh5BG536900 Rh5DG548500
rosa_wichuraiana	Rw2G039040 Rw5G047700 Rw5G047720 Rw5G047860

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	1 cut(s)	496
AciI	CCGC	4 cut(s)	12, 108, 111, 458
AcoI	YGGCCR	2 cut(s)	3, 298
AcsI	RAATTY	3 cut(s)	346, 595, 608
AcuI	CTGAAG	1 cut(s)	201
AfaI	GTAC	1 cut(s)	415
AfiI	CCNNNNNNNGG	3 cut(s)	133, 261, 329
AflIII	ACRYGT	1 cut(s)	494
AgsI	TTSAA	5 cut(s)	75, 179, 189, 247, 420
AjnI	CCWGG	1 cut(s)	127
AluBI	AGCT	1 cut(s)	26
AluI	AGCT	1 cut(s)	26
Alw26I	GTCTC	1 cut(s)	66
AoxI	GGCC	3 cut(s)	3, 298, 567
ApoI	RAATTY	3 cut(s)	346, 595, 608
AspLEI	GCGC	1 cut(s)	690
AspS9I	GGNCC	1 cut(s)	568
AsuC2I	CCSGG	1 cut(s)	262
AsuHPI	GGTGA	2 cut(s)	245, 458
AxyI	CCTNAGG	1 cut(s)	533
BalI	TGGCCA	2 cut(s)	5, 300
BarI	GAAGNNNNNNTAC	2 cut(s)	400, 432
BbsI	GAAGAC	1 cut(s)	158
BccI	CCATC	4 cut(s)	176, 518, 623, 733
BceAI	ACGGC	1 cut(s)	718
BciT130I	CCWGG	1 cut(s)	129
BcnI	CCSGG	1 cut(s)	262
BcoDI	GTCTC	1 cut(s)	66
BfaI	CTAG	3 cut(s)	165, 288, 389
BfoI	RGCGCY	1 cut(s)	691
BisI	GCNGC	1 cut(s)	111
BlsI	GCNGC	1 cut(s)	112
BmcAI	AGTACT	1 cut(s)	415
Bme1390I	CCNGG	2 cut(s)	129, 262
BmgT120I	GGNCC	1 cut(s)	568
BmiI	GGNNCC	2 cut(s)	223, 570
BmrFI	CCNGG	2 cut(s)	129, 262
BmsI	GCATC	2 cut(s)	122, 151
BpiI	GAAGAC	1 cut(s)	158
BpmI	CTGGAG	1 cut(s)	214
BpuMI	CCSGG	1 cut(s)	262
BsaJI	CCNNGG	4 cut(s)	127, 146, 379, 700
Bsc4I	CCNNNNNNNGG	3 cut(s)	133, 261, 329
Bse1I	ACTGG	2 cut(s)	301, 343
Bse21I	CCTNAGG	1 cut(s)	533
Bse3DI	GCAATG	1 cut(s)	377
BseBI	CCWGG	1 cut(s)	129
BseDI	CCNNGG	4 cut(s)	127, 146, 379, 700
BseGI	GGATG	2 cut(s)	166, 251
BseLI	CCNNNNNNNGG	3 cut(s)	133, 261, 329
BseMI	GCAATG	1 cut(s)	377
BseMII	CTCAG	1 cut(s)	109
BseNI	ACTGG	2 cut(s)	301, 343
BseRI	GAGGAG	1 cut(s)	94
BsgI	GTGCAG	1 cut(s)	453
Bsh1236I	CGCG	1 cut(s)	496
BshFI	GGCC	3 cut(s)	5, 300, 569
BsiSI	CCGG	1 cut(s)	261
BslFI	GGGAC	1 cut(s)	110
BslI	CCNNNNNNNGG	3 cut(s)	133, 261, 329
BsmAI	GTCTC	1 cut(s)	66
BsmFI	GGGAC	1 cut(s)	110
BsnI	GGCC	3 cut(s)	5, 300, 569
Bsp19I	CCATGG	1 cut(s)	146
BspACI	CCGC	4 cut(s)	12, 108, 111, 458
BspANI	GGCC	3 cut(s)	5, 300, 569
BspCNI	CTCAG	1 cut(s)	108
BspFNI	CGCG	1 cut(s)	496
BspLI	GGNNCC	2 cut(s)	223, 570
BsrDI	GCAATG	1 cut(s)	377
BsrI	ACTGG	2 cut(s)	301, 343
BssECI	CCNNGG	4 cut(s)	127, 146, 379, 700
BssT1I	CCWWGG	2 cut(s)	146, 379
Bst2UI	CCWGG	1 cut(s)	129
Bst4CI	ACNGT	1 cut(s)	718
Bst6I	CTCTTC	1 cut(s)	619
BstC8I	GCNNGC	2 cut(s)	298, 498
BstDEI	CTNAG	6 cut(s)	22, 80, 95, 201, 533, 683
BstDSI	CCRYGG	2 cut(s)	146, 700
BstF5I	GGATG	2 cut(s)	166, 251
BstFNI	CGCG	1 cut(s)	496
BstH2I	RGCGCY	1 cut(s)	691
BstHHI	GCGC	1 cut(s)	690
BstMAI	GTCTC	1 cut(s)	66
BstMWI	GCNNNNNNNGC	1 cut(s)	11
BstNI	CCWGG	1 cut(s)	129
BstNSI	RCATGY	1 cut(s)	564
BstSCI	CCNGG	2 cut(s)	127, 260
BstUI	CGCG	1 cut(s)	496
BstV2I	GAAGAC	1 cut(s)	158
Bsu36I	CCTNAGG	1 cut(s)	533
BsuRI	GGCC	3 cut(s)	5, 300, 569
BtgI	CCRYGG	2 cut(s)	146, 700
BtsCI	GGATG	2 cut(s)	166, 251
Cac8I	GCNNGC	2 cut(s)	298, 498
CfoI	GCGC	1 cut(s)	690
Cfr13I	GGNCC	1 cut(s)	568
CseI	GACGC	1 cut(s)	108
Csp6I	GTAC	1 cut(s)	414
CviAII	CATG	4 cut(s)	147, 472, 561, 644
CviJI	RGCY	9 cut(s)	5, 26, 200, 300, 531, 569, 638, 695, 705
CviKI_1	RGCY	9 cut(s)	5, 26, 200, 300, 531, 569, 638, 695, 705
CviQI	GTAC	1 cut(s)	414
DdeI	CTNAG	6 cut(s)	22, 80, 95, 201, 533, 683
EaeI	YGGCCR	2 cut(s)	3, 298
Eam1104I	CTCTTC	1 cut(s)	619
EarI	CTCTTC	1 cut(s)	619
EciI	GGCGGA	1 cut(s)	97
Eco130I	CCWWGG	2 cut(s)	146, 379
Eco57I	CTGAAG	1 cut(s)	201
Eco81I	CCTNAGG	1 cut(s)	533
EcoO109I	RGGNCCY	1 cut(s)	568
EcoRII	CCWGG	1 cut(s)	127
EcoT14I	CCWWGG	2 cut(s)	146, 379
ErhI	CCWWGG	2 cut(s)	146, 379
FaeI	CATG	4 cut(s)	150, 475, 564, 647
FalI	AAGNNNNNCTT	2 cut(s)	359, 391
FaqI	GGGAC	1 cut(s)	110
FatI	CATG	4 cut(s)	146, 471, 560, 643
FauNDI	CATATG	1 cut(s)	655
Fnu4HI	GCNGC	1 cut(s)	111
FokI	GGATG	2 cut(s)	173, 238
Fsp4HI	GCNGC	1 cut(s)	111
FspBI	CTAG	3 cut(s)	165, 288, 389
GlaI	GCGC	1 cut(s)	689
GluI	GCNGC	1 cut(s)	111
GsuI	CTGGAG	1 cut(s)	214
HaeII	RGCGCY	1 cut(s)	691
HaeIII	GGCC	3 cut(s)	5, 300, 569
HapII	CCGG	1 cut(s)	261
HgaI	GACGC	1 cut(s)	108
HhaI	GCGC	1 cut(s)	690
Hin1II	CATG	4 cut(s)	150, 475, 564, 647
Hin6I	GCGC	1 cut(s)	688
HinP1I	GCGC	1 cut(s)	688
HincII	GTYRAC	3 cut(s)	19, 42, 490
HindII	GTYRAC	3 cut(s)	19, 42, 490
HinfI	GANTC	2 cut(s)	362, 666
HpaII	CCGG	1 cut(s)	261
HphI	GGTGA	2 cut(s)	245, 458
Hpy166II	GTNNAC	3 cut(s)	19, 42, 490
Hpy188I	TCNGA	3 cut(s)	98, 160, 601
Hpy188III	TCNNGA	3 cut(s)	193, 219, 269
Hpy8I	GTNNAC	3 cut(s)	19, 42, 490
HpyAV	CCTTC	1 cut(s)	81
HpyCH4III	ACNGT	1 cut(s)	718
HpyCH4V	TGCA	4 cut(s)	434, 510, 564, 653
HpyF10VI	GCNNNNNNNGC	1 cut(s)	11
HpyF3I	CTNAG	6 cut(s)	22, 80, 95, 201, 533, 683
Hsp92II	CATG	4 cut(s)	150, 475, 564, 647
HspAI	GCGC	1 cut(s)	688
LmnI	GCTCC	1 cut(s)	107
LpnPI	CCDG	8 cut(s)	114, 141, 178, 204, 274, 282, 314, 324
LweI	GCATC	2 cut(s)	122, 151
MaeI	CTAG	3 cut(s)	165, 288, 389
MaeIII	GTNAC	1 cut(s)	556
MboII	GAAGA	4 cut(s)	163, 191, 371, 636
MlsI	TGGCCA	2 cut(s)	5, 300
MluCI	AATT	5 cut(s)	346, 421, 595, 608, 711
MluI	ACGCGT	1 cut(s)	494
MluNI	TGGCCA	2 cut(s)	5, 300
MnlI	CCTC	4 cut(s)	115, 189, 456, 628
Mox20I	TGGCCA	2 cut(s)	5, 300
MscI	TGGCCA	2 cut(s)	5, 300
MseI	TTAA	3 cut(s)	404, 515, 612
Msp20I	TGGCCA	2 cut(s)	5, 300
MspA1I	CMGCKG	1 cut(s)	14
MspI	CCGG	1 cut(s)	261
MspR9I	CCNGG	2 cut(s)	129, 262
MvaI	CCWGG	1 cut(s)	129
MvnI	CGCG	1 cut(s)	496
MwoI	GCNNNNNNNGC	1 cut(s)	11
NciI	CCSGG	1 cut(s)	262
NcoI	CCATGG	1 cut(s)	146
NdeI	CATATG	1 cut(s)	655
NlaIII	CATG	4 cut(s)	150, 475, 564, 647
NlaIV	GGNNCC	2 cut(s)	223, 570
NmuCI	GTSAC	1 cut(s)	556
NspI	RCATGY	1 cut(s)	564
PfeI	GAWTC	2 cut(s)	362, 666
PflFI	GACNNNGTC	1 cut(s)	46
PkrI	GCNGC	1 cut(s)	112
Psp6I	CCWGG	1 cut(s)	127
PspGI	CCWGG	1 cut(s)	127
PspN4I	GGNNCC	2 cut(s)	223, 570
PspPI	GGNCC	1 cut(s)	568
PsyI	GACNNNGTC	1 cut(s)	46
RsaI	GTAC	1 cut(s)	415
RsaNI	GTAC	1 cut(s)	414
SaqAI	TTAA	3 cut(s)	404, 515, 612
SatI	GCNGC	1 cut(s)	111
Sau96I	GGNCC	1 cut(s)	568
ScaI	AGTACT	1 cut(s)	415
ScrFI	CCNGG	2 cut(s)	129, 262
SetI	ASST	2 cut(s)	28, 133
SfaNI	GCATC	2 cut(s)	122, 151
Sse9I	AATT	5 cut(s)	346, 421, 595, 608, 711
SsiI	CCGC	4 cut(s)	12, 108, 111, 458
SspI	AATATT	1 cut(s)	277
SspMI	CTAG	3 cut(s)	165, 288, 389
StyD4I	CCNGG	2 cut(s)	127, 260
StyI	CCWWGG	2 cut(s)	146, 379
TaaI	ACNGT	1 cut(s)	718
TasI	AATT	5 cut(s)	346, 421, 595, 608, 711
TatI	WGTACW	1 cut(s)	413
TauI	GCSGC	1 cut(s)	113
TfiI	GAWTC	2 cut(s)	362, 666
Tru1I	TTAA	3 cut(s)	404, 515, 612
Tru9I	TTAA	3 cut(s)	404, 515, 612
TseFI	GTSAC	1 cut(s)	556
Tsp45I	GTSAC	1 cut(s)	556
TspDTI	ATGAA	2 cut(s)	591, 632
Tth111I	GACNNNGTC	1 cut(s)	46
XapI	RAATTY	3 cut(s)	346, 595, 608
XceI	RCATGY	1 cut(s)	564
XspI	CTAG	3 cut(s)	165, 288, 389
ZrmI	AGTACT	1 cut(s)	415

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.