Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5B

Orthogroup ID

OG0003046

Physical Location & Seq

Genomic Coordinates Forward (+)

84728101 .. 84731499

Gene Structure 3399 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5BG536900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1284 bp

ATGGCCACAGCAGCTATCAACTTATTCCTCGTAAAGCCGTCGCCTTTCTCTGTTCCCAAGCCTCAAAACCCTTCAAAGCTTAGGGTTTTCACTCTCCGATGCTCCTCCGCCACCGCCAATGCCGTCTCAGACTTGTCCGAAAGACCTTGGAAGACTTCAGATGCTAGACTTGTGCTTGAAGATGGTTCAATATGGAGGGCAAAGTCATTTGGTGCTTCTGGAACCCAAGTTGGTGAAGTTGTATTCAATACATCCTTGACCGGGTATCAAGAAATTATTACAGACCCGAGTTATGCTGGCCAATTTGTCCTGATGACAAACCCCCAAATTGGAAATACTGGTGTAAATTTTGATGATGAAGAATCAAGGCAGTGCTTCCTTGGTGGTCTAGTGATCAGAAGTTTAAGTATCAGTACTTCAAATCATAGATGTGCAGAAACTCTTGGTGATTATTTAGCAAAAAGGAATATCATGGGAATTTATGATGTTGACACACGGGCTATCACCCGCAGATTAAGGCAAGATGGAAGCCTTATTGGTGTTTTGAGCACAGAAGAGACCAAAACAGATGAGGAACTATTGGAAATGTCTCGTTCATGGGACATTGTTGGGGTTGATTTAATAAGTGGCGTGTCATGCAAGACCCCTCATGAGTGGGTTGATAAAACCAATTCTGAGTGGGAATTTATGTCCAAAAGAAAAGATGGAGAGGCTTTTCGTGTTGTTGCATATGATTTTGGAATCAAGAGCAACATACTTAGGCGATTGGCTTCCTATGGCTGCAAAATTACTGTGGTACCTTCAACATGGCCAGCATCAGAGACTCTTAAGATGAAACCAGATGGGGTCCTTTTCAGCAATGGCCCCGGAGACCCATCTGCAGTTCCCTATGCTGTTGAAACGGTCAAGCAGATTCTTGGAAAGGTTCCTGTTTTTGGAATTTGCATGGGCCATCAGTTGCTTGGCTTGGCATTAGGCGGAACAACTTTTAAAATGAAGTTCGGTCACCACGGAGGAAATCATCCAGTCCGTAATCTTCGTACTGGAGATGTTGAGATAAGCGCCCAGAATCACAACTATGCGGTTGATCCCAAATCACTTCCAGATGGTGTAGAAGTAACTCATGTAAATCTCAACGATGGAAGCTGCGCTGGCCTTGCTTATCCTGCACGAAACATCATGTCTATTCAGTACCATCCTGAAGCATCCCCAGGACCTCATGATTCAGATTATGCTTTTAGAGAATTCATTGAGCTGATGAAGAGAGTGAAAGTAAATGCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000050 GO:0003674 GO:0003824 GO:0004088 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005951 GO:0006082 GO:0006520 GO:0006525 GO:0006526 GO:0006807 GO:0006950 GO:0007154 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009064 GO:0009084 GO:0009267 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009987 GO:0009991 GO:0016036 GO:0016053 GO:0016874 GO:0016879 GO:0016884 GO:0019627 GO:0019752 GO:0031667 GO:0031668 GO:0031669 GO:0032991 GO:0033554 GO:0034641 GO:0042594 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0050896 GO:0051716 GO:0071496 GO:0071704 GO:0071941 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607 GO:1902494

KEGG Pathways

Metabolic & Signaling

ko00240 ko00250 ko01100 map00240 map00250 map01100

Pfam Domains

Protein Families

CPSase_sm_chain GATase

Protein Analysis

427

Amino Acids

46.8

Weight (kDa)

6.57

Isoelectric Point (pI)

45.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CPSase_sm_chain	PF00988	57 - 181	4.7e-47	Carbamoyl-phosphate synthase small chain, CPSase domain
GATase	PF00117	243 - 418	2.3e-55	Glutamine amidotransferase class-I

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003046)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G27740
fragaria_vesca	FvH4_3g43670 FvH4_3g43670 FvH4_5g29030 FvH4_5g29030 FvH4_5g29030
malus_domestica	MD03G1020600.v1.1 MD11G1021800.v1.1 MD15G1342500.v1.1
prunus_persica	Prupe.6G016000_v2.0.a1 Prupe.6G016000_v2.0.a1 Prupe.6G163300_v2.0.a1
pyrus_communis	pycom03g01750 pycom11g01770
rosa_chinensis	RchiOBHm_Chr2g0150151 RchiOBHm_Chr5g0078331
rosa_laevigata	RLG00000020471 RLG00000036765 RLG00000036767
rosa_multiflora	Rmu_sc0000631.1_g000032 Rmu_sc0009445.1_g000001 Rmu_ssc0000020.1_g000013
rosa_roxburghii	Rroxscaffold_1G00003910 Rroxscaffold_1G00003930 Rroxscaffold_2G00097400
rosa_rugosa	Rorug02G0419400 Rorug05G0460000
rosa_samantha	Rh2AG478200 Rh2CG464400 Rh2DG499600 Rh5AG513900 Rh5AG514000 Rh5BG536900 Rh5DG548500
rosa_wichuraiana	Rw2G039040 Rw5G047700 Rw5G047720 Rw5G047860

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	796
AccB1I	GGYRCC	1 cut(s)	796
AciI	CCGC	5 cut(s)	108, 114, 508, 978, 1082
AclWI	GGATC	1 cut(s)	1082
AcoI	YGGCCR	3 cut(s)	3, 298, 809
AcsI	RAATTY	5 cut(s)	346, 477, 683, 939, 1244
AcuI	CTGAAG	2 cut(s)	141, 1221
AfaI	GTAC	4 cut(s)	415, 798, 1042, 1193
AfiI	CCNNNNNNNGG	3 cut(s)	261, 329, 935
AflII	CTTAAG	1 cut(s)	827
AgsI	TTSAA	7 cut(s)	75, 179, 189, 247, 420, 804, 899
AjnI	CCWGG	1 cut(s)	1210
AluBI	AGCT	4 cut(s)	14, 79, 1146, 1255
AluI	AGCT	4 cut(s)	14, 79, 1146, 1255
Alw21I	GWGCWC	1 cut(s)	551
Alw26I	GTCTC	5 cut(s)	130, 551, 594, 815, 864
AlwI	GGATC	1 cut(s)	1082
Ama87I	CYCGRG	1 cut(s)	286
AoxI	GGCC	6 cut(s)	3, 298, 809, 862, 949, 1153
ApeKI	GCWGC	3 cut(s)	11, 780, 1146
ApoI	RAATTY	5 cut(s)	346, 477, 683, 939, 1244
Asp718I	GGTACC	1 cut(s)	796
AspLEI	GCGC	2 cut(s)	1064, 1151
AspS9I	GGNCC	4 cut(s)	847, 863, 949, 1214
AsuC2I	CCSGG	2 cut(s)	262, 867
AsuHPI	GGTGA	4 cut(s)	245, 458, 496, 998
AvaI	CYCGRG	1 cut(s)	286
AvaII	GGWCC	2 cut(s)	847, 1214
BaeI	ACNNNNGTAYC	2 cut(s)	780, 813
BalI	TGGCCA	3 cut(s)	5, 300, 811
BanI	GGYRCC	1 cut(s)	796
BarI	GAAGNNNNNNTAC	2 cut(s)	400, 432
BbsI	GAAGAC	1 cut(s)	158
Bbv12I	GWGCWC	1 cut(s)	551
BbvI	GCAGC	3 cut(s)	23, 767, 1133
BccI	CCATC	9 cut(s)	176, 518, 698, 836, 883, 960, 1100, 1133, 1203
BceAI	ACGGC	2 cut(s)	22, 107
BciT130I	CCWGG	1 cut(s)	1212
BclI	TGATCA	1 cut(s)	393
BcnI	CCSGG	2 cut(s)	262, 867
BcoDI	GTCTC	5 cut(s)	130, 551, 594, 815, 864
BfaI	CTAG	2 cut(s)	165, 389
BfmI	CTRYAG	1 cut(s)	879
BfoI	RGCGCY	1 cut(s)	1065
BfrI	CTTAAG	1 cut(s)	827
BisI	GCNGC	3 cut(s)	12, 781, 1147
BlsI	GCNGC	3 cut(s)	13, 782, 1148
BmcAI	AGTACT	1 cut(s)	415
Bme1390I	CCNGG	3 cut(s)	262, 867, 1212
Bme18I	GGWCC	2 cut(s)	847, 1214
BmeT110I	CYCGRG	1 cut(s)	286
BmgT120I	GGNCC	4 cut(s)	847, 863, 949, 1214
BmiI	GGNNCC	5 cut(s)	223, 798, 848, 865, 927
BmrFI	CCNGG	3 cut(s)	262, 867, 1212
BmsI	GCATC	4 cut(s)	89, 151, 824, 1214
BpiI	GAAGAC	1 cut(s)	158
BpmI	CTGGAG	1 cut(s)	1065
Bpu10I	CCTNAGC	1 cut(s)	80
BpuMI	CCSGG	2 cut(s)	262, 867
BsaI	GGTCTC	2 cut(s)	551, 864
BsaJI	CCNNGG	5 cut(s)	146, 379, 865, 1009, 1210
Bsc4I	CCNNNNNNNGG	3 cut(s)	261, 329, 935
Bse1I	ACTGG	3 cut(s)	343, 1025, 1048
Bse3DI	GCAATG	1 cut(s)	865
BseBI	CCWGG	1 cut(s)	1212
BseDI	CCNNGG	5 cut(s)	146, 379, 865, 1009, 1210
BseGI	GGATG	4 cut(s)	251, 1021, 1195, 1205
BseLI	CCNNNNNNNGG	3 cut(s)	261, 329, 935
BseMI	GCAATG	1 cut(s)	865
BseMII	CTCAG	2 cut(s)	141, 666
BseNI	ACTGG	3 cut(s)	343, 1025, 1048
BseRI	GAGGAG	1 cut(s)	94
BseXI	GCAGC	3 cut(s)	23, 767, 1133
BsgI	GTGCAG	2 cut(s)	453, 1152
BshFI	GGCC	6 cut(s)	5, 300, 811, 864, 951, 1155
BshNI	GGYRCC	1 cut(s)	796
BsiHKAI	GWGCWC	1 cut(s)	551
BsiHKCI	CYCGRG	1 cut(s)	286
BsiSI	CCGG	2 cut(s)	261, 867
BslFI	GGGAC	1 cut(s)	614
BslI	CCNNNNNNNGG	3 cut(s)	261, 329, 935
BsmAI	GTCTC	5 cut(s)	130, 551, 594, 815, 864
BsmBI	CGTCTC	1 cut(s)	130
BsmFI	GGGAC	1 cut(s)	614
BsnI	GGCC	6 cut(s)	5, 300, 811, 864, 951, 1155
Bso31I	GGTCTC	2 cut(s)	551, 864
BsoBI	CYCGRG	1 cut(s)	286
Bsp1286I	GDGCHC	1 cut(s)	551
Bsp143I	GATC	2 cut(s)	393, 1087
BspACI	CCGC	5 cut(s)	108, 114, 508, 978, 1082
BspANI	GGCC	6 cut(s)	5, 300, 811, 864, 951, 1155
BspCNI	CTCAG	2 cut(s)	140, 667
BspHI	TCATGA	2 cut(s)	649, 1219
BspLI	GGNNCC	5 cut(s)	223, 798, 848, 865, 927
BspMAI	CTGCAG	1 cut(s)	883
BspPI	GGATC	1 cut(s)	1082
BspT107I	GGYRCC	1 cut(s)	796
BspTI	CTTAAG	1 cut(s)	827
BspTNI	GGTCTC	2 cut(s)	551, 864
BsrDI	GCAATG	1 cut(s)	865
BsrI	ACTGG	3 cut(s)	343, 1025, 1048
BssECI	CCNNGG	5 cut(s)	146, 379, 865, 1009, 1210
BssMI	GATC	2 cut(s)	393, 1087
BssT1I	CCWWGG	2 cut(s)	146, 379
Bst2UI	CCWGG	1 cut(s)	1212
Bst4CI	ACNGT	2 cut(s)	793, 904
Bst6I	CTCTTC	2 cut(s)	549, 1256
BstAFI	CTTAAG	1 cut(s)	827
BstC8I	GCNNGC	3 cut(s)	298, 813, 1153
BstDEI	CTNAG	4 cut(s)	80, 127, 675, 758
BstDSI	CCRYGG	1 cut(s)	1009
BstEII	GGTNACC	1 cut(s)	1004
BstF5I	GGATG	4 cut(s)	251, 1021, 1195, 1205
BstH2I	RGCGCY	1 cut(s)	1065
BstHHI	GCGC	2 cut(s)	1064, 1151
BstKTI	GATC	2 cut(s)	396, 1090
BstMAI	GTCTC	5 cut(s)	130, 551, 594, 815, 864
BstMBI	GATC	2 cut(s)	393, 1087
BstMWI	GCNNNNNNNGC	5 cut(s)	11, 636, 1152, 1157, 1166
BstNI	CCWGG	1 cut(s)	1212
BstPI	GGTNACC	1 cut(s)	1004
BstSCI	CCNGG	3 cut(s)	260, 865, 1210
BstSFI	CTRYAG	1 cut(s)	879
BstV1I	GCAGC	3 cut(s)	23, 767, 1133
BstV2I	GAAGAC	1 cut(s)	158
BsuRI	GGCC	6 cut(s)	5, 300, 811, 864, 951, 1155
BtgI	CCRYGG	1 cut(s)	1009
BtsCI	GGATG	4 cut(s)	251, 1021, 1195, 1205
BtsI	GCAGTG	1 cut(s)	377
BtsIMutI	CAGTG	1 cut(s)	377
Cac8I	GCNNGC	3 cut(s)	298, 813, 1153
CciI	TCATGA	2 cut(s)	649, 1219
CfoI	GCGC	2 cut(s)	1064, 1151
Cfr13I	GGNCC	4 cut(s)	847, 863, 949, 1214
Csp6I	GTAC	4 cut(s)	414, 797, 1041, 1192
CviQI	GTAC	4 cut(s)	414, 797, 1041, 1192
DdeI	CTNAG	4 cut(s)	80, 127, 675, 758
DpnI	GATC	2 cut(s)	395, 1089
DpnII	GATC	2 cut(s)	393, 1087
DraI	TTTAAA	1 cut(s)	991
EaeI	YGGCCR	3 cut(s)	3, 298, 809
Eam1104I	CTCTTC	2 cut(s)	549, 1256
EarI	CTCTTC	2 cut(s)	549, 1256
EciI	GGCGGA	2 cut(s)	97, 993
Eco130I	CCWWGG	2 cut(s)	146, 379
Eco31I	GGTCTC	2 cut(s)	551, 864
Eco47I	GGWCC	2 cut(s)	847, 1214
Eco57I	CTGAAG	2 cut(s)	141, 1221
Eco88I	CYCGRG	1 cut(s)	286
Eco91I	GGTNACC	1 cut(s)	1004
EcoO109I	RGGNCCY	2 cut(s)	847, 1214
EcoO65I	GGTNACC	1 cut(s)	1004
EcoRI	GAATTC	1 cut(s)	1244
EcoRII	CCWGG	1 cut(s)	1210
EcoT14I	CCWWGG	2 cut(s)	146, 379
EcoT22I	ATGCAT	1 cut(s)	1282
ErhI	CCWWGG	2 cut(s)	146, 379
Esp3I	CGTCTC	1 cut(s)	130
FaqI	GGGAC	1 cut(s)	614
FauI	CCCGC	1 cut(s)	515
FauNDI	CATATG	1 cut(s)	730
FbaI	TGATCA	1 cut(s)	393
Fnu4HI	GCNGC	3 cut(s)	12, 781, 1147
FokI	GGATG	4 cut(s)	238, 1008, 1182, 1192
Fsp4HI	GCNGC	3 cut(s)	12, 781, 1147
FspBI	CTAG	2 cut(s)	165, 389
GlaI	GCGC	2 cut(s)	1063, 1150
GluI	GCNGC	3 cut(s)	12, 781, 1147
GsuI	CTGGAG	1 cut(s)	1065
HaeII	RGCGCY	1 cut(s)	1065
HaeIII	GGCC	6 cut(s)	5, 300, 811, 864, 951, 1155
HapII	CCGG	2 cut(s)	261, 867
HhaI	GCGC	2 cut(s)	1064, 1151
Hin6I	GCGC	2 cut(s)	1062, 1149
HinP1I	GCGC	2 cut(s)	1062, 1149
HincII	GTYRAC	1 cut(s)	490
HindII	GTYRAC	1 cut(s)	490
HindIII	AAGCTT	1 cut(s)	77
HinfI	GANTC	6 cut(s)	362, 741, 823, 913, 1069, 1223
HpaII	CCGG	2 cut(s)	261, 867
HphI	GGTGA	4 cut(s)	245, 458, 496, 998
Hpy166II	GTNNAC	1 cut(s)	490
Hpy188I	TCNGA	8 cut(s)	98, 130, 139, 160, 398, 676, 820, 1228
Hpy188III	TCNNGA	8 cut(s)	219, 269, 310, 650, 745, 1103, 1199, 1220
Hpy8I	GTNNAC	1 cut(s)	490
Hpy99I	CGWCG	1 cut(s)	43
HpyAV	CCTTC	2 cut(s)	81, 810
HpyCH4III	ACNGT	2 cut(s)	793, 904
HpyCH4V	TGCA	8 cut(s)	434, 639, 728, 783, 881, 945, 1169, 1280
HpyF10VI	GCNNNNNNNGC	5 cut(s)	11, 636, 1152, 1157, 1166
HpyF3I	CTNAG	4 cut(s)	80, 127, 675, 758
HspAI	GCGC	2 cut(s)	1062, 1149
KpnI	GGTACC	1 cut(s)	800
Ksp22I	TGATCA	1 cut(s)	393
Kzo9I	GATC	2 cut(s)	393, 1087
LmnI	GCTCC	1 cut(s)	107
Lsp1109I	GCAGC	3 cut(s)	23, 767, 1133
LweI	GCATC	4 cut(s)	89, 151, 824, 1214
MaeI	CTAG	2 cut(s)	165, 389
MaeIII	GTNAC	2 cut(s)	1004, 1117
MalI	GATC	2 cut(s)	395, 1089
MboI	GATC	2 cut(s)	393, 1087
MboII	GAAGA	6 cut(s)	163, 191, 371, 566, 1028, 1273
MhlI	GDGCHC	1 cut(s)	551
MlsI	TGGCCA	3 cut(s)	5, 300, 811
MluNI	TGGCCA	3 cut(s)	5, 300, 811
MlyI	GAGTC	1 cut(s)	817
MnlI	CCTC	9 cut(s)	38, 72, 115, 189, 565, 657, 703, 1007, 1227
Mox20I	TGGCCA	3 cut(s)	5, 300, 811
Mph1103I	ATGCAT	1 cut(s)	1282
MscI	TGGCCA	3 cut(s)	5, 300, 811
MseI	TTAA	5 cut(s)	404, 515, 620, 828, 990
MslI	CAYNNNNRTG	2 cut(s)	429, 1077
Msp20I	TGGCCA	3 cut(s)	5, 300, 811
MspCI	CTTAAG	1 cut(s)	827
MspI	CCGG	2 cut(s)	261, 867
MspR9I	CCNGG	3 cut(s)	262, 867, 1212
MvaI	CCWGG	1 cut(s)	1212
MwoI	GCNNNNNNNGC	5 cut(s)	11, 636, 1152, 1157, 1166
NciI	CCSGG	2 cut(s)	262, 867
NdeI	CATATG	1 cut(s)	730
NdeII	GATC	2 cut(s)	393, 1087
NlaIV	GGNNCC	5 cut(s)	223, 798, 848, 865, 927
NmuCI	GTSAC	1 cut(s)	1004
NsiI	ATGCAT	1 cut(s)	1282
PagI	TCATGA	2 cut(s)	649, 1219
PfeI	GAWTC	5 cut(s)	362, 741, 913, 1069, 1223
PkrI	GCNGC	3 cut(s)	13, 782, 1148
PleI	GAGTC	1 cut(s)	817
PpsI	GAGTC	1 cut(s)	817
PpuMI	RGGWCCY	2 cut(s)	847, 1214
Psp5II	RGGWCCY	2 cut(s)	847, 1214
Psp6I	CCWGG	1 cut(s)	1210
PspEI	GGTNACC	1 cut(s)	1004
PspGI	CCWGG	1 cut(s)	1210
PspN4I	GGNNCC	5 cut(s)	223, 798, 848, 865, 927
PspPI	GGNCC	4 cut(s)	847, 863, 949, 1214
PspPPI	RGGWCCY	2 cut(s)	847, 1214
PstI	CTGCAG	1 cut(s)	883
RsaI	GTAC	4 cut(s)	415, 798, 1042, 1193
RsaNI	GTAC	4 cut(s)	414, 797, 1041, 1192
RseI	CAYNNNNRTG	2 cut(s)	429, 1077
SaqAI	TTAA	5 cut(s)	404, 515, 620, 828, 990
SatI	GCNGC	3 cut(s)	12, 781, 1147
Sau3AI	GATC	2 cut(s)	393, 1087
Sau96I	GGNCC	4 cut(s)	847, 863, 949, 1214
ScaI	AGTACT	1 cut(s)	415
SchI	GAGTC	1 cut(s)	817
ScrFI	CCNGG	3 cut(s)	262, 867, 1212
SduI	GDGCHC	1 cut(s)	551
SetI	ASST	8 cut(s)	16, 81, 148, 802, 927, 1148, 1219, 1257
SfaNI	GCATC	4 cut(s)	89, 151, 824, 1214
SfcI	CTRYAG	1 cut(s)	879
SinI	GGWCC	2 cut(s)	847, 1214
SmiMI	CAYNNNNRTG	2 cut(s)	429, 1077
SmlI	CTYRAG	1 cut(s)	827
SmoI	CTYRAG	1 cut(s)	827
SsiI	CCGC	5 cut(s)	108, 114, 508, 978, 1082
SspMI	CTAG	2 cut(s)	165, 389
StyD4I	CCNGG	3 cut(s)	260, 865, 1210
StyI	CCWWGG	2 cut(s)	146, 379
TaaI	ACNGT	2 cut(s)	793, 904
TaqII	GACCGA	1 cut(s)	992
TatI	WGTACW	1 cut(s)	413
TfiI	GAWTC	5 cut(s)	362, 741, 913, 1069, 1223
Tru1I	TTAA	5 cut(s)	404, 515, 620, 828, 990
Tru9I	TTAA	5 cut(s)	404, 515, 620, 828, 990
TscAI	CASTG	1 cut(s)	377
TseFI	GTSAC	1 cut(s)	1004
TseI	GCWGC	3 cut(s)	11, 780, 1146
Tsp45I	GTSAC	1 cut(s)	1004
TspDTI	ATGAA	6 cut(s)	372, 585, 848, 1010, 1237, 1274
TspGWI	ACGGA	2 cut(s)	1019, 1026
TspRI	CASTG	1 cut(s)	377
Vha464I	CTTAAG	1 cut(s)	827
VpaK11BI	GGWCC	2 cut(s)	847, 1214
XapI	RAATTY	5 cut(s)	346, 477, 683, 939, 1244
XcmI	CCANNNNNNNNNTGG	1 cut(s)	959
XspI	CTAG	2 cut(s)	165, 389
ZrmI	AGTACT	1 cut(s)	415
Zsp2I	ATGCAT	1 cut(s)	1282

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.