Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0002198

Physical Location & Seq

Genomic Coordinates Forward (+)

3192551 .. 3197506

Gene Structure 4956 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1040300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1344 bp

ATGAATTATAAGCCTGGAATCCAGTCTCAACTTGAAATGGAATCACTGGGTAACGGACCAATACATGAGTTTGAGGATGAGAGTCTTTTTACTCCAAAGACAGAGGCTCAAAAGACACGTTCTATTGAAGCAGTGGGAGAATGTTCGTTTTCAGGAGGTTCACAAGCTAGCGGTGATAAAGTGTGGAGGAATATTGTTTATAGAAGCATAAAGACTGTTGTTTTCTCAACTAAACTGAACTTGCTTATGCCTTTTGGGCCGCTAGCGATACTTGTCTATAAGCTAACCGGCCATAATGGTTGGGTCTTTGTTTTGAGCTTGTTGGGTATAACACCTTTAGCTGAGCGTTTGGGTTATGCTACTGAGCAGCTGGCATTCTATACTGGAGCTACTGTTGGGGGTCTTTTAAATGCTACTTTTGGAAATGCAACAGAACTGATTATATCAATTTATGCACTGAAACGTGGAATGTTACGCGTTGTTCAGCAGTCGTTATTAGGCTCTATTCTGTCAAACATGTTGCTGGTGCTTGGTTGTGCATTCTTCAGTGGTGGACTTGTTTTTCAAGAGAAAGAACAGGTGTTTAACAAGGCAACTGCTGTTGTGAACTCTGGCTTGCTGCTAATGGCAGTCATGGGCCTACTTTTTCCAGCTGTCTTGCACTACACTCACACTGAGGTTAATTTCGGGAAGTCAGAGTTGGCACTTTCAAGATTTAGCAGCTGTATCATGCTCCTGGCATATGCTGCTTTTCTTTTCTTCCAGTTAAAGAGTCGGCATAATCAATATGTGCCTTTAAATGAGGAAGGGATTCAGAATGAAGAGAACGCAGATGACGATGAAGCTCCTGAGATCTCTAAATGGGAATCAATAATTTGGCTATCAATAATGACTGCTTGGATCTCAATCGTATCCGAATATTTAGTTAACGCCATAGAGGGAGCATCTATAGCATGGAACATGCCAATTTCATTTATCAGCGTTATTTTGCTCCCAATTGTGGGTAATGCTGCAGAGCATGCCAGTGCTATTATGTTTGCCATGAAGGACAAGCTTGACATTTCTTTGGGAGTGGCAATAGGGTCGTCAACGCAAATATCCATGTTTGGGATTCCTTTCTGCGTGGTTGTTGGGTGGATTATGGGACGCCCTATGGACTTGAACTTTCAGCTTTTTGAGACGGCAACACTTTTCATAACTGTCTTAGTTGTAGCCTTCTTGCTGCAGGAGGGAACTTCTAATTACTTCAAAGGGTTGATGCTTATTCTTTGTTATCTGATAGTTGCTGCAAGTTTCTTTGTTCACGTAGATCCTTCTCCGGCAACAGGTACGCCCCCCTTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000325 GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0008150 GO:0008324 GO:0009705 GO:0015075 GO:0015077 GO:0015078 GO:0015085 GO:0015291 GO:0015297 GO:0015298 GO:0015299 GO:0015318 GO:0015368 GO:0015369 GO:0015491 GO:0015672 GO:0016020 GO:0022804 GO:0022857 GO:0022890 GO:0030001 GO:0031090 GO:0034220 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046873 GO:0051139 GO:0051179 GO:0051234 GO:0055085 GO:0070588 GO:0070838 GO:0072511 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098805 GO:0099516 GO:1902600

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Na_Ca_ex

Protein Analysis

448

Amino Acids

48.92

Weight (kDa)

5.17

Isoelectric Point (pI)

38.03

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Na_Ca_ex	PF01699	101 - 256	4.5e-22	Sodium/calcium exchanger protein
Na_Ca_ex	PF01699	290 - 432	3.1e-23	Sodium/calcium exchanger protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002198)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G55730 AT1G55730 AT3G13320
fragaria_vesca	FvH4_3g41890 FvH4_3g41890 FvH4_3g41890 FvH4_3g41890 FvH4_3g41890 FvH4_3g41890 FvH4_3g41890 FvH4_3g41890 FvH4_6g40200 FvH4_6g40200 FvH4_6g40200
malus_domestica	MD03G1040300.v1.1 MD09G1134300.v1.1 MD11G1041700.v1.1 MD17G1123100.v1.1
prunus_persica	Prupe.3G192400_v2.0.a1 Prupe.6G032600_v2.0.a1 Prupe.6G032600_v2.0.a1 Prupe.6G032600_v2.0.a1
pyrus_communis	pycom03g03100 pycom11g03540
rosa_chinensis	RchiOBHm_Chr2g0155221 RchiOBHm_Chr5g0075081
rosa_laevigata	RLG00000020827 RLG00000036528
rosa_multiflora	Rmu_sc0006984.1_g000024 Rmu_sc0012066.1_g000002 Rmu_sc0028924.1_g000002
rosa_roxburghii	Rroxscaffold_1G00006540 Rroxscaffold_2G00093860
rosa_rugosa	Rorug02G0446100 Rorug02G0446100 Rorug05G0436000 Rorug05G0436100
rosa_samantha	Rh2AG512500 Rh2CG497500 Rh5AG493500 Rh5CG538700
rosa_wichuraiana	Rw2G042100 Rw5G045760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	9
AccII	CGCG	1 cut(s)	477
AciI	CCGC	2 cut(s)	171, 260
AclWI	GGATC	2 cut(s)	908, 1304
AcoI	YGGCCR	1 cut(s)	289
AcuI	CTGAAG	1 cut(s)	529
AcyI	GRCGYC	1 cut(s)	1147
AfaI	GTAC	1 cut(s)	1330
AfiI	CCNNNNNNNGG	4 cut(s)	1000, 1001, 1107, 1325
AflIII	ACRYGT	3 cut(s)	116, 475, 516
AgsI	TTSAA	6 cut(s)	35, 128, 566, 711, 1162, 1249
AjnI	CCWGG	2 cut(s)	13, 735
AjuI	GAANNNNNNNTTGG	2 cut(s)	683, 715
Alw26I	GTCTC	2 cut(s)	30, 1172
AlwI	GGATC	2 cut(s)	908, 1304
AoxI	GGCC	3 cut(s)	257, 289, 637
ApeKI	GCWGC	7 cut(s)	367, 619, 720, 746, 1010, 1222, 1286
Asp700I	GAANNNNTTC	1 cut(s)	810
AspS9I	GGNCC	3 cut(s)	56, 257, 637
AsuHPI	GGTGA	1 cut(s)	185
AsuNHI	GCTAGC	2 cut(s)	167, 262
AvaII	GGWCC	1 cut(s)	56
BbvI	GCAGC	7 cut(s)	379, 606, 732, 733, 997, 1209, 1273
BceAI	ACGGC	1 cut(s)	1197
BcgI	CGANNNNNNTGC	2 cut(s)	1065, 1099
BciT130I	CCWGG	2 cut(s)	15, 737
BciVI	GTATCC	1 cut(s)	922
BcoDI	GTCTC	2 cut(s)	30, 1172
BfaI	CTAG	3 cut(s)	168, 263, 1342
BfmI	CTRYAG	3 cut(s)	948, 1011, 1223
BfuI	GTATCC	1 cut(s)	922
BglI	GCCNNNNNGGC	1 cut(s)	256
BglII	AGATCT	1 cut(s)	852
BisI	GCNGC	8 cut(s)	260, 368, 620, 721, 747, 1011, 1223, 1287
BlpI	GCTNAGC	1 cut(s)	342
BlsI	GCNGC	8 cut(s)	261, 369, 621, 722, 748, 1012, 1224, 1288
Bme1390I	CCNGG	2 cut(s)	15, 737
Bme18I	GGWCC	1 cut(s)	56
BmgT120I	GGNCC	3 cut(s)	56, 257, 637
BmrFI	CCNGG	2 cut(s)	15, 737
BmrI	ACTGGG	1 cut(s)	56
BmsI	GCATC	2 cut(s)	953, 1248
BmtI	GCTAGC	2 cut(s)	171, 266
BmuI	ACTGGG	1 cut(s)	56
BpmI	CTGGAG	1 cut(s)	405
Bpu1102I	GCTNAGC	1 cut(s)	342
BsaAI	YACGTR	1 cut(s)	1306
BsaBI	GATNNNNATC	1 cut(s)	905
BsaHI	GRCGYC	1 cut(s)	1147
BsaXI	ACNNNNNCTCC	2 cut(s)	378, 408
Bsc4I	CCNNNNNNNGG	4 cut(s)	1000, 1001, 1107, 1325
Bse118I	RCCGGY	1 cut(s)	287
Bse1I	ACTGG	5 cut(s)	22, 51, 388, 763, 1023
Bse8I	GATNNNNATC	1 cut(s)	905
BseBI	CCWGG	2 cut(s)	15, 737
BseGI	GGATG	1 cut(s)	82
BseJI	GATNNNNATC	1 cut(s)	905
BseLI	CCNNNNNNNGG	4 cut(s)	1000, 1001, 1107, 1325
BseMII	CTCAG	4 cut(s)	333, 354, 666, 840
BseNI	ACTGG	5 cut(s)	22, 51, 388, 763, 1023
BseXI	GCAGC	7 cut(s)	379, 606, 732, 733, 997, 1209, 1273
Bsh1236I	CGCG	1 cut(s)	477
BshFI	GGCC	3 cut(s)	259, 291, 639
BsiSI	CCGG	2 cut(s)	288, 1319
BslFI	GGGAC	1 cut(s)	1158
BslI	CCNNNNNNNGG	4 cut(s)	1000, 1001, 1107, 1325
BsmAI	GTCTC	2 cut(s)	30, 1172
BsmBI	CGTCTC	1 cut(s)	1172
BsmFI	GGGAC	1 cut(s)	1158
BsmI	GAATGC	2 cut(s)	374, 539
BsnI	GGCC	3 cut(s)	259, 291, 639
Bsp143I	GATC	3 cut(s)	852, 900, 1309
Bsp1720I	GCTNAGC	1 cut(s)	342
BspACI	CCGC	2 cut(s)	171, 260
BspANI	GGCC	3 cut(s)	259, 291, 639
BspCNI	CTCAG	4 cut(s)	334, 355, 667, 841
BspFNI	CGCG	1 cut(s)	477
BspMAI	CTGCAG	2 cut(s)	1015, 1227
BspOI	GCTAGC	2 cut(s)	171, 266
BspPI	GGATC	2 cut(s)	908, 1304
BsrFI	RCCGGY	1 cut(s)	287
BsrI	ACTGG	5 cut(s)	22, 51, 388, 763, 1023
BssAI	RCCGGY	1 cut(s)	287
BssMI	GATC	3 cut(s)	852, 900, 1309
BssNI	GRCGYC	1 cut(s)	1147
Bst2UI	CCWGG	2 cut(s)	15, 737
Bst4CI	ACNGT	3 cut(s)	217, 394, 1201
Bst6I	CTCTTC	1 cut(s)	816
BstACI	GRCGYC	1 cut(s)	1147
BstAPI	GCANNNNNTGC	2 cut(s)	746, 1019
BstBAI	YACGTR	1 cut(s)	1306
BstC8I	GCNNGC	5 cut(s)	169, 264, 372, 617, 1020
BstDEI	CTNAG	5 cut(s)	342, 363, 675, 849, 1204
BstF5I	GGATG	1 cut(s)	82
BstFNI	CGCG	1 cut(s)	477
BstKTI	GATC	3 cut(s)	855, 903, 1312
BstMAI	GTCTC	2 cut(s)	30, 1172
BstMBI	GATC	3 cut(s)	852, 900, 1309
BstMWI	GCNNNNNNNGC	4 cut(s)	256, 746, 950, 1019
BstNI	CCWGG	2 cut(s)	15, 737
BstNSI	RCATGY	3 cut(s)	520, 964, 1022
BstSCI	CCNGG	2 cut(s)	13, 735
BstSFI	CTRYAG	3 cut(s)	948, 1011, 1223
BstUI	CGCG	1 cut(s)	477
BstV1I	GCAGC	7 cut(s)	379, 606, 732, 733, 997, 1209, 1273
BstX2I	RGATCY	3 cut(s)	852, 900, 1309
BstYI	RGATCY	3 cut(s)	852, 900, 1309
BsuI	GTATCC	1 cut(s)	922
BsuRI	GGCC	3 cut(s)	259, 291, 639
BtsCI	GGATG	1 cut(s)	82
BtsI	GCAGTG	1 cut(s)	138
BtsIMutI	CAGTG	6 cut(s)	44, 138, 455, 553, 672, 1030
Cac8I	GCNNGC	5 cut(s)	169, 264, 372, 617, 1020
Cfr10I	RCCGGY	1 cut(s)	287
Cfr13I	GGNCC	3 cut(s)	56, 257, 637
CseI	GACGC	1 cut(s)	1155
Csp6I	GTAC	1 cut(s)	1329
CviAII	CATG	9 cut(s)	65, 517, 634, 730, 954, 961, 1019, 1042, 1102
CviQI	GTAC	1 cut(s)	1329
DdeI	CTNAG	5 cut(s)	342, 363, 675, 849, 1204
DpnI	GATC	3 cut(s)	854, 902, 1311
DpnII	GATC	3 cut(s)	852, 900, 1309
DraI	TTTAAA	2 cut(s)	408, 798
EaeI	YGGCCR	1 cut(s)	289
Eam1104I	CTCTTC	1 cut(s)	816
EarI	CTCTTC	1 cut(s)	816
Eco47I	GGWCC	1 cut(s)	56
Eco57I	CTGAAG	1 cut(s)	529
EcoRII	CCWGG	2 cut(s)	13, 735
Esp3I	CGTCTC	1 cut(s)	1172
FaeI	CATG	9 cut(s)	68, 520, 637, 733, 957, 964, 1022, 1045, 1105
FaqI	GGGAC	1 cut(s)	1158
FatI	CATG	9 cut(s)	64, 516, 633, 729, 953, 960, 1018, 1041, 1101
FauNDI	CATATG	1 cut(s)	742
Fnu4HI	GCNGC	8 cut(s)	260, 368, 620, 721, 747, 1011, 1223, 1287
FokI	GGATG	1 cut(s)	89
Fsp4HI	GCNGC	8 cut(s)	260, 368, 620, 721, 747, 1011, 1223, 1287
FspBI	CTAG	3 cut(s)	168, 263, 1342
GluI	GCNGC	8 cut(s)	260, 368, 620, 721, 747, 1011, 1223, 1287
GsuI	CTGGAG	1 cut(s)	405
HaeIII	GGCC	3 cut(s)	259, 291, 639
HapII	CCGG	2 cut(s)	288, 1319
HgaI	GACGC	1 cut(s)	1155
Hin1I	GRCGYC	1 cut(s)	1147
Hin1II	CATG	9 cut(s)	68, 520, 637, 733, 957, 964, 1022, 1045, 1105
HincII	GTYRAC	2 cut(s)	928, 1089
HindII	GTYRAC	2 cut(s)	928, 1089
HindIII	AAGCTT	1 cut(s)	1052
HinfI	GANTC	7 cut(s)	18, 41, 82, 772, 811, 866, 1111
HpaI	GTTAAC	1 cut(s)	928
HpaII	CCGG	2 cut(s)	288, 1319
HphI	GGTGA	1 cut(s)	185
Hpy166II	GTNNAC	6 cut(s)	161, 554, 607, 928, 1089, 1303
Hpy188I	TCNGA	4 cut(s)	697, 816, 916, 1278
Hpy188III	TCNNGA	5 cut(s)	153, 566, 688, 711, 848
Hpy8I	GTNNAC	6 cut(s)	161, 554, 607, 928, 1089, 1303
HpyAV	CCTTC	4 cut(s)	800, 1039, 1225, 1323
HpyCH4III	ACNGT	3 cut(s)	217, 394, 1201
HpyCH4IV	ACGT	3 cut(s)	118, 463, 1305
HpyCH4V	TGCA	7 cut(s)	428, 455, 539, 661, 1013, 1225, 1289
HpyF10VI	GCNNNNNNNGC	4 cut(s)	256, 746, 950, 1019
HpyF3I	CTNAG	5 cut(s)	342, 363, 675, 849, 1204
HpySE526I	ACGT	3 cut(s)	118, 463, 1305
Hsp92I	GRCGYC	1 cut(s)	1147
Hsp92II	CATG	9 cut(s)	68, 520, 637, 733, 957, 964, 1022, 1045, 1105
KspAI	GTTAAC	1 cut(s)	928
Kzo9I	GATC	3 cut(s)	852, 900, 1309
LmnI	GCTCC	5 cut(s)	386, 738, 850, 941, 996
Lsp1109I	GCAGC	7 cut(s)	379, 606, 732, 733, 997, 1209, 1273
LweI	GCATC	2 cut(s)	953, 1248
MaeI	CTAG	3 cut(s)	168, 263, 1342
MaeII	ACGT	3 cut(s)	118, 463, 1305
MaeIII	GTNAC	2 cut(s)	50, 471
MalI	GATC	3 cut(s)	854, 902, 1311
MboI	GATC	3 cut(s)	852, 900, 1309
MboII	GAAGA	3 cut(s)	535, 751, 833
MfeI	CAATTG	1 cut(s)	996
MflI	RGATCY	3 cut(s)	852, 900, 1309
MluCI	AATT	7 cut(s)	4, 447, 682, 873, 966, 996, 1240
MluI	ACGCGT	1 cut(s)	475
MlyI	GAGTC	2 cut(s)	91, 781
MnlI	CCTC	8 cut(s)	67, 97, 149, 180, 670, 796, 931, 1222
MroXI	GAANNNNTTC	1 cut(s)	810
MseI	TTAA	6 cut(s)	407, 585, 681, 767, 797, 927
MslI	CAYNNNNRTG	1 cut(s)	1023
MspA1I	CMGCKG	3 cut(s)	370, 653, 723
MspI	CCGG	2 cut(s)	288, 1319
MspR9I	CCNGG	2 cut(s)	15, 737
MunI	CAATTG	1 cut(s)	996
Mva1269I	GAATGC	2 cut(s)	374, 539
MvaI	CCWGG	2 cut(s)	15, 737
MvnI	CGCG	1 cut(s)	477
MwoI	GCNNNNNNNGC	4 cut(s)	256, 746, 950, 1019
NdeI	CATATG	1 cut(s)	742
NdeII	GATC	3 cut(s)	852, 900, 1309
NheI	GCTAGC	2 cut(s)	167, 262
NlaIII	CATG	9 cut(s)	68, 520, 637, 733, 957, 964, 1022, 1045, 1105
NspI	RCATGY	3 cut(s)	520, 964, 1022
PaeI	GCATGC	1 cut(s)	1022
PciI	ACATGT	1 cut(s)	516
PcsI	WCGNNNNNNNCGW	1 cut(s)	834
PctI	GAATGC	2 cut(s)	374, 539
PdmI	GAANNNNTTC	1 cut(s)	810
PfeI	GAWTC	5 cut(s)	18, 41, 811, 866, 1111
PkrI	GCNGC	8 cut(s)	261, 369, 621, 722, 748, 1012, 1224, 1288
PleI	GAGTC	2 cut(s)	90, 780
PpsI	GAGTC	2 cut(s)	90, 780
Ppu21I	YACGTR	1 cut(s)	1306
PscI	ACATGT	1 cut(s)	516
PsiI	TTATAA	1 cut(s)	9
Psp6I	CCWGG	2 cut(s)	13, 735
PspGI	CCWGG	2 cut(s)	13, 735
PspPI	GGNCC	3 cut(s)	56, 257, 637
PstI	CTGCAG	2 cut(s)	1015, 1227
PsuI	RGATCY	3 cut(s)	852, 900, 1309
PvuII	CAGCTG	3 cut(s)	370, 653, 723
RsaI	GTAC	1 cut(s)	1330
RsaNI	GTAC	1 cut(s)	1329
RseI	CAYNNNNRTG	1 cut(s)	1023
SaqAI	TTAA	6 cut(s)	407, 585, 681, 767, 797, 927
SatI	GCNGC	8 cut(s)	260, 368, 620, 721, 747, 1011, 1223, 1287
Sau3AI	GATC	3 cut(s)	852, 900, 1309
Sau96I	GGNCC	3 cut(s)	56, 257, 637
SchI	GAGTC	2 cut(s)	91, 781
ScrFI	CCNGG	2 cut(s)	15, 737
SfaNI	GCATC	2 cut(s)	953, 1248
SfcI	CTRYAG	3 cut(s)	948, 1011, 1223
SinI	GGWCC	1 cut(s)	56
SmiMI	CAYNNNNRTG	1 cut(s)	1023
SphI	GCATGC	1 cut(s)	1022
Sse9I	AATT	7 cut(s)	4, 447, 682, 873, 966, 996, 1240
SsiI	CCGC	2 cut(s)	171, 260
SspI	AATATT	2 cut(s)	193, 920
SspMI	CTAG	3 cut(s)	168, 263, 1342
StyD4I	CCNGG	2 cut(s)	13, 735
TaaI	ACNGT	3 cut(s)	217, 394, 1201
TaiI	ACGT	3 cut(s)	121, 466, 1308
TasI	AATT	7 cut(s)	4, 447, 682, 873, 966, 996, 1240
TauI	GCSGC	1 cut(s)	262
TfiI	GAWTC	5 cut(s)	18, 41, 811, 866, 1111
Tru1I	TTAA	6 cut(s)	407, 585, 681, 767, 797, 927
Tru9I	TTAA	6 cut(s)	407, 585, 681, 767, 797, 927
TscAI	CASTG	6 cut(s)	51, 138, 462, 553, 679, 1030
TseI	GCWGC	7 cut(s)	367, 619, 720, 746, 1010, 1222, 1286
TspDTI	ATGAA	6 cut(s)	17, 834, 855, 960, 1058, 1183
TspGWI	ACGGA	1 cut(s)	69
TspRI	CASTG	6 cut(s)	51, 138, 462, 553, 679, 1030
VpaK11BI	GGWCC	1 cut(s)	56
XceI	RCATGY	3 cut(s)	520, 964, 1022
XmnI	GAANNNNTTC	1 cut(s)	810
XspI	CTAG	3 cut(s)	168, 263, 1342

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.