MD03G1049900.v1.1

Pathogenesis-related genes transcriptional activator PTI6-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0009231
Physical Location & Seq
Forward (+)
3981184 .. 3982008
825 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1049900.v1.1.491

Sequence Viewer

Length: 825 bp
ATGACAACCTTCCAACCCATCTCGTCCGTACACTCAACGGTCAAATTTTCAGAGCAAATAACCACCGTGAAAAAACCCATGATCCAGGAACCCGATAACCCGACTCGAATCCCGCCAAAGCTCGTCCGGATCATACTTACGGATGCCGACGCCACCGACTCCTCCAGCGACGACGAAGACAGAGGCGTGCGCAGAGTCAAACGACATGTTAGACAGATCAGCCTAGAGCACTCCTGGCCTTCCTCTTCCTCTCCTTCATCTTCGTCGTCGGTTTCTTCGGGTCAGTCGAAGCGGGCGAGGAAAGACTCGCGACCCGGATCGAAGTCACCGGAAACGACCGCGGATCGCCGGAAAAAGTTTAGAGGCGTCCGGCGGAGGCCTTGGGGTCGCTGGGCCGCGGAGATCCGAGACCCGACCCGAAGAAAACGGATCTGGCTCGGGACCTTCGACACGGCGGAGGAAGCCGCCACTGTTTACGACAGAGCTGCCATCTTGCTCAAAGGTCCTGACGCCGTCACCAACTTTTCTAACGCCGTTATAAAGGAGGAAACAGTGGTTGGCGACTTGACGGTCCCAGCTGCGGGGCAGGGATGTGGGTGTGTATCTCCGTCGTCCAGCGAGGCTCTCTCGTCGCCTACTTCGGTCCTCCGTTACGGTGACCAAACGCCGTTTGATGGTTTAAGTTACGGATACACTGACGCCTTCAAATTTGATTTCGACTTGCCGTTAAGCTTGCCGGATATCATGTTTTCAAGTAGCAGCTTTGCGCGCGACGAGTTCGGCGAGTTTGATGTTGACGATTTCTTGGCTGACGTCGTATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
AP2

Protein Analysis

275

Amino Acids

30.24

Weight (kDa)

6.85

Isoelectric Point (pI)

59.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 119 - 168 5.7e-15 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 539
AasI GACNNNNNNGTC 1 cut(s) 569
AatII GACGTC 1 cut(s) 816
Acc16I TGCGCA 1 cut(s) 191
AccII CGCG 5 cut(s) 310, 341, 398, 769, 771
AccIII TCCGGA 1 cut(s) 126
AclWI GGATC 6 cut(s) 76, 137, 325, 351, 397, 437
AcsI RAATTY 2 cut(s) 44, 707
AcyI GRCGYC 5 cut(s) 150, 366, 510, 699, 813
AfaI GTAC 1 cut(s) 30
AfiI CCNNNNNNNGG 3 cut(s) 580, 581, 674
AflIII ACRYGT 1 cut(s) 205
AgsI TTSAA 2 cut(s) 706, 753
AjnI CCWGG 2 cut(s) 84, 233
AjuI GAANNNNNNNTTGG 2 cut(s) 540, 572
AluBI AGCT 5 cut(s) 121, 485, 578, 732, 762
AluI AGCT 5 cut(s) 121, 485, 578, 732, 762
Alw21I GWGCWC 1 cut(s) 231
Alw26I GTCTC 1 cut(s) 402
AlwI GGATC 6 cut(s) 76, 137, 325, 351, 397, 437
Ama87I CYCGRG 1 cut(s) 437
Aor13HI TCCGGA 1 cut(s) 126
AoxI GGCC 3 cut(s) 236, 377, 393
ApeKI GCWGC 3 cut(s) 485, 578, 759
ApoI RAATTY 2 cut(s) 44, 707
AspLEI GCGC 3 cut(s) 192, 769, 771
AspS9I GGNCC 5 cut(s) 393, 441, 503, 571, 643
AsuC2I CCSGG 1 cut(s) 315
AsuHPI GGTGA 3 cut(s) 318, 508, 668
AvaI CYCGRG 1 cut(s) 437
AvaII GGWCC 4 cut(s) 441, 503, 571, 643
BbsI GAAGAC 1 cut(s) 183
Bbv12I GWGCWC 1 cut(s) 231
BbvI GCAGC 3 cut(s) 472, 565, 771
BccI CCATC 3 cut(s) 26, 497, 668
BceAI ACGGC 5 cut(s) 468, 497, 518, 652, 709
BcgI CGANNNNNNTGC 2 cut(s) 467, 501
BciT130I CCWGG 2 cut(s) 86, 235
BciVI GTATCC 1 cut(s) 683
BcnI CCSGG 1 cut(s) 315
BcoDI GTCTC 1 cut(s) 402
BfaI CTAG 1 cut(s) 224
BfuI GTATCC 1 cut(s) 683
BisI GCNGC 5 cut(s) 396, 465, 486, 579, 760
BlsI GCNGC 5 cut(s) 397, 466, 487, 580, 761
Bme1390I CCNGG 3 cut(s) 86, 235, 315
Bme18I GGWCC 4 cut(s) 441, 503, 571, 643
BmeT110I CYCGRG 1 cut(s) 437
BmgT120I GGNCC 5 cut(s) 393, 441, 503, 571, 643
BmiI GGNNCC 3 cut(s) 90, 442, 573
BmrFI CCNGG 3 cut(s) 86, 235, 315
BmsI GCATC 1 cut(s) 133
BpiI GAAGAC 1 cut(s) 183
BplI GAGNNNNNCTC 2 cut(s) 611, 643
BpmI CTGGAG 1 cut(s) 148
BpuMI CCSGG 1 cut(s) 315
BsaHI GRCGYC 5 cut(s) 150, 366, 510, 699, 813
BsaI GGTCTC 1 cut(s) 402
BsaJI CCNNGG 3 cut(s) 339, 380, 396
BsaWI WCCGGW 2 cut(s) 126, 328
Bsc4I CCNNNNNNNGG 3 cut(s) 580, 581, 674
BseAI TCCGGA 1 cut(s) 126
BseBI CCWGG 2 cut(s) 86, 235
BseDI CCNNGG 3 cut(s) 339, 380, 396
BseGI GGATG 2 cut(s) 148, 596
BseLI CCNNNNNNNGG 3 cut(s) 580, 581, 674
BsePI GCGCGC 1 cut(s) 767
BseRI GAGGAG 1 cut(s) 151
BseXI GCAGC 3 cut(s) 472, 565, 771
BseYI CCCAGC 2 cut(s) 390, 574
Bsh1236I CGCG 5 cut(s) 310, 341, 398, 769, 771
Bsh1285I CGRYCG 1 cut(s) 339
BshFI GGCC 3 cut(s) 238, 379, 395
BsiEI CGRYCG 1 cut(s) 339
BsiHKAI GWGCWC 1 cut(s) 231
BsiHKCI CYCGRG 1 cut(s) 437
BsiSI CCGG 6 cut(s) 127, 315, 329, 349, 370, 737
BslFI GGGAC 2 cut(s) 454, 557
BslI CCNNNNNNNGG 3 cut(s) 580, 581, 674
BsmAI GTCTC 1 cut(s) 402
BsmFI GGGAC 2 cut(s) 454, 557
BsnI GGCC 3 cut(s) 238, 379, 395
Bso31I GGTCTC 1 cut(s) 402
BsoBI CYCGRG 1 cut(s) 437
Bsp1286I GDGCHC 1 cut(s) 231
Bsp13I TCCGGA 1 cut(s) 126
Bsp143I GATC 7 cut(s) 81, 129, 216, 317, 343, 402, 429
Bsp68I TCGCGA 1 cut(s) 310
BspANI GGCC 3 cut(s) 238, 379, 395
BspEI TCCGGA 1 cut(s) 126
BspFNI CGCG 5 cut(s) 310, 341, 398, 769, 771
BspLI GGNNCC 3 cut(s) 90, 442, 573
BspPI GGATC 6 cut(s) 76, 137, 325, 351, 397, 437
BspTNI GGTCTC 1 cut(s) 402
BssECI CCNNGG 3 cut(s) 339, 380, 396
BssHII GCGCGC 1 cut(s) 767
BssMI GATC 7 cut(s) 81, 129, 216, 317, 343, 402, 429
BssNI GRCGYC 5 cut(s) 150, 366, 510, 699, 813
BssT1I CCWWGG 1 cut(s) 380
Bst2UI CCWGG 2 cut(s) 86, 235
Bst4CI ACNGT 6 cut(s) 40, 67, 472, 553, 571, 656
Bst6I CTCTTC 1 cut(s) 250
BstACI GRCGYC 5 cut(s) 150, 366, 510, 699, 813
BstC8I GCNNGC 4 cut(s) 188, 294, 734, 769
BstDSI CCRYGG 2 cut(s) 339, 396
BstEII GGTNACC 1 cut(s) 656
BstF5I GGATG 2 cut(s) 148, 596
BstFNI CGCG 5 cut(s) 310, 341, 398, 769, 771
BstHHI GCGC 3 cut(s) 192, 769, 771
BstKTI GATC 7 cut(s) 84, 132, 219, 320, 346, 405, 432
BstMAI GTCTC 1 cut(s) 402
BstMBI GATC 7 cut(s) 81, 129, 216, 317, 343, 402, 429
BstMCI CGRYCG 1 cut(s) 339
BstMWI GCNNNNNNNGC 3 cut(s) 235, 461, 768
BstNI CCWGG 2 cut(s) 86, 235
BstNSI RCATGY 1 cut(s) 209
BstPI GGTNACC 1 cut(s) 656
BstSCI CCNGG 3 cut(s) 84, 233, 313
BstUI CGCG 5 cut(s) 310, 341, 398, 769, 771
BstV1I GCAGC 3 cut(s) 472, 565, 771
BstV2I GAAGAC 1 cut(s) 183
BstX2I RGATCY 2 cut(s) 402, 429
BstYI RGATCY 2 cut(s) 402, 429
BsuI GTATCC 1 cut(s) 683
BsuRI GGCC 3 cut(s) 238, 379, 395
BtgI CCRYGG 2 cut(s) 339, 396
BtsCI GGATG 2 cut(s) 148, 596
BtsIMutI CAGTG 3 cut(s) 468, 558, 693
BtuMI TCGCGA 1 cut(s) 310
Cac8I GCNNGC 4 cut(s) 188, 294, 734, 769
CfoI GCGC 3 cut(s) 192, 769, 771
Cfr13I GGNCC 5 cut(s) 393, 441, 503, 571, 643
Cfr42I CCGCGG 2 cut(s) 342, 399
CseI GACGC 4 cut(s) 158, 355, 518, 707
Csp6I GTAC 1 cut(s) 29
CviAII CATG 3 cut(s) 79, 206, 745
CviQI GTAC 1 cut(s) 29
DpnI GATC 7 cut(s) 83, 131, 218, 319, 345, 404, 431
DpnII GATC 7 cut(s) 81, 129, 216, 317, 343, 402, 429
DrdI GACNNNNNNGTC 1 cut(s) 569
DseDI GACNNNNNNGTC 1 cut(s) 569
Eam1104I CTCTTC 1 cut(s) 250
EarI CTCTTC 1 cut(s) 250
EciI GGCGGA 2 cut(s) 388, 470
Eco130I CCWWGG 1 cut(s) 380
Eco147I AGGCCT 1 cut(s) 379
Eco31I GGTCTC 1 cut(s) 402
Eco32I GATATC 1 cut(s) 742
Eco47I GGWCC 4 cut(s) 441, 503, 571, 643
Eco88I CYCGRG 1 cut(s) 437
Eco91I GGTNACC 1 cut(s) 656
EcoO109I RGGNCCY 2 cut(s) 441, 503
EcoO65I GGTNACC 1 cut(s) 656
EcoRII CCWGG 2 cut(s) 84, 233
EcoRV GATATC 1 cut(s) 742
EcoT14I CCWWGG 1 cut(s) 380
ErhI CCWWGG 1 cut(s) 380
FaeI CATG 3 cut(s) 82, 209, 748
FaiI YATR 6 cut(s) 80, 134, 207, 539, 746, 820
FaqI GGGAC 2 cut(s) 454, 557
FatI CATG 3 cut(s) 78, 205, 744
FauI CCCGC 3 cut(s) 120, 285, 574
Fnu4HI GCNGC 5 cut(s) 396, 465, 486, 579, 760
FokI GGATG 2 cut(s) 155, 603
Fsp4HI GCNGC 5 cut(s) 396, 465, 486, 579, 760
FspBI CTAG 1 cut(s) 224
FspI TGCGCA 1 cut(s) 191
GlaI GCGC 3 cut(s) 191, 768, 770
GluI GCNGC 5 cut(s) 396, 465, 486, 579, 760
GsaI CCCAGC 2 cut(s) 394, 578
GsuI CTGGAG 1 cut(s) 148
HaeIII GGCC 3 cut(s) 238, 379, 395
HapII CCGG 6 cut(s) 127, 315, 329, 349, 370, 737
HgaI GACGC 4 cut(s) 158, 355, 518, 707
HhaI GCGC 3 cut(s) 192, 769, 771
Hin1I GRCGYC 5 cut(s) 150, 366, 510, 699, 813
Hin1II CATG 3 cut(s) 82, 209, 748
Hin6I GCGC 3 cut(s) 190, 767, 769
HinP1I GCGC 3 cut(s) 190, 767, 769
HincII GTYRAC 1 cut(s) 796
HindII GTYRAC 1 cut(s) 796
HindIII AAGCTT 1 cut(s) 730
HinfI GANTC 5 cut(s) 103, 108, 158, 195, 305
HpaII CCGG 6 cut(s) 127, 315, 329, 349, 370, 737
HphI GGTGA 3 cut(s) 318, 508, 668
Hpy166II GTNNAC 3 cut(s) 31, 475, 796
Hpy188I TCNGA 2 cut(s) 52, 407
Hpy188III TCNNGA 4 cut(s) 127, 309, 439, 506
Hpy8I GTNNAC 3 cut(s) 31, 475, 796
Hpy99I CGWCG 9 cut(s) 152, 173, 176, 268, 271, 613, 634, 776, 818
HpyAV CCTTC 5 cut(s) 19, 249, 264, 454, 712
HpyCH4III ACNGT 6 cut(s) 40, 67, 472, 553, 571, 656
HpyCH4IV ACGT 1 cut(s) 813
HpyF10VI GCNNNNNNNGC 3 cut(s) 235, 461, 768
HpySE526I ACGT 1 cut(s) 813
Hsp92I GRCGYC 5 cut(s) 150, 366, 510, 699, 813
Hsp92II CATG 3 cut(s) 82, 209, 748
HspAI GCGC 3 cut(s) 190, 767, 769
Kpn2I TCCGGA 1 cut(s) 126
KspI CCGCGG 2 cut(s) 342, 399
Kzo9I GATC 7 cut(s) 81, 129, 216, 317, 343, 402, 429
Lsp1109I GCAGC 3 cut(s) 472, 565, 771
LweI GCATC 1 cut(s) 133
MaeI CTAG 1 cut(s) 224
MaeII ACGT 1 cut(s) 813
MaeIII GTNAC 5 cut(s) 324, 514, 650, 656, 683
MalI GATC 7 cut(s) 83, 131, 218, 319, 345, 404, 431
MboI GATC 7 cut(s) 81, 129, 216, 317, 343, 402, 429
MboII GAAGA 5 cut(s) 188, 237, 252, 267, 432
MflI RGATCY 2 cut(s) 402, 429
MhlI GDGCHC 1 cut(s) 231
MluCI AATT 2 cut(s) 44, 707
MlyI GAGTC 4 cut(s) 97, 152, 204, 299
MmeI TCCRAC 1 cut(s) 37
MroI TCCGGA 1 cut(s) 126
MseI TTAA 2 cut(s) 680, 728
MspA1I CMGCKG 3 cut(s) 341, 398, 578
MspI CCGG 6 cut(s) 127, 315, 329, 349, 370, 737
MspR9I CCNGG 3 cut(s) 86, 235, 315
MvaI CCWGG 2 cut(s) 86, 235
MvnI CGCG 5 cut(s) 310, 341, 398, 769, 771
MwoI GCNNNNNNNGC 3 cut(s) 235, 461, 768
NciI CCSGG 1 cut(s) 315
NdeII GATC 7 cut(s) 81, 129, 216, 317, 343, 402, 429
NlaIII CATG 3 cut(s) 82, 209, 748
NlaIV GGNNCC 3 cut(s) 90, 442, 573
NmuCI GTSAC 3 cut(s) 324, 514, 656
NruI TCGCGA 1 cut(s) 310
NsbI TGCGCA 1 cut(s) 191
NspI RCATGY 1 cut(s) 209
PauI GCGCGC 1 cut(s) 767
PceI AGGCCT 1 cut(s) 379
PciI ACATGT 1 cut(s) 205
PcsI WCGNNNNNNNCGW 4 cut(s) 284, 293, 444, 780
PfeI GAWTC 1 cut(s) 108
PflFI GACNNNGTC 1 cut(s) 512
PfoI TCCNGGA 1 cut(s) 84
PkrI GCNGC 5 cut(s) 397, 466, 487, 580, 761
PleI GAGTC 4 cut(s) 97, 152, 203, 299
PpsI GAGTC 4 cut(s) 97, 152, 203, 299
PpuMI RGGWCCY 2 cut(s) 441, 503
PscI ACATGT 1 cut(s) 205
PsiI TTATAA 1 cut(s) 539
Psp5II RGGWCCY 2 cut(s) 441, 503
Psp6I CCWGG 2 cut(s) 84, 233
PspEI GGTNACC 1 cut(s) 656
PspFI CCCAGC 2 cut(s) 390, 574
PspGI CCWGG 2 cut(s) 84, 233
PspN4I GGNNCC 3 cut(s) 90, 442, 573
PspPI GGNCC 5 cut(s) 393, 441, 503, 571, 643
PspPPI RGGWCCY 2 cut(s) 441, 503
PsuI RGATCY 2 cut(s) 402, 429
PsyI GACNNNGTC 1 cut(s) 512
PteI GCGCGC 1 cut(s) 767
PvuII CAGCTG 1 cut(s) 578
RruI TCGCGA 1 cut(s) 310
RsaI GTAC 1 cut(s) 30
RsaNI GTAC 1 cut(s) 29
SacII CCGCGG 2 cut(s) 342, 399
SaqAI TTAA 2 cut(s) 680, 728
SatI GCNGC 5 cut(s) 396, 465, 486, 579, 760
Sau3AI GATC 7 cut(s) 81, 129, 216, 317, 343, 402, 429
Sau96I GGNCC 5 cut(s) 393, 441, 503, 571, 643
SchI GAGTC 4 cut(s) 97, 152, 204, 299
ScrFI CCNGG 3 cut(s) 86, 235, 315
SduI GDGCHC 1 cut(s) 231
SetI ASST 9 cut(s) 11, 123, 446, 487, 505, 580, 734, 764, 816
SfaNI GCATC 1 cut(s) 133
Sfr303I CCGCGG 2 cut(s) 342, 399
SgrBI CCGCGG 2 cut(s) 342, 399
SinI GGWCC 4 cut(s) 441, 503, 571, 643
Sse9I AATT 2 cut(s) 44, 707
SseBI AGGCCT 1 cut(s) 379
SspMI CTAG 1 cut(s) 224
StuI AGGCCT 1 cut(s) 379
StyD4I CCNGG 3 cut(s) 84, 233, 313
StyI CCWWGG 1 cut(s) 380
TaaI ACNGT 6 cut(s) 40, 67, 472, 553, 571, 656
TaiI ACGT 1 cut(s) 816
TaqI TCGA 5 cut(s) 106, 287, 320, 447, 717
TaqII GACCGA 1 cut(s) 631
TasI AATT 2 cut(s) 44, 707
TauI GCSGC 2 cut(s) 398, 467
TfiI GAWTC 1 cut(s) 108
Tru1I TTAA 2 cut(s) 680, 728
Tru9I TTAA 2 cut(s) 680, 728
TscAI CASTG 3 cut(s) 475, 558, 700
TseFI GTSAC 3 cut(s) 324, 514, 656
TseI GCWGC 3 cut(s) 485, 578, 759
Tsp45I GTSAC 3 cut(s) 324, 514, 656
TspDTI ATGAA 1 cut(s) 246
TspGWI ACGGA 6 cut(s) 16, 155, 442, 597, 638, 702
TspRI CASTG 3 cut(s) 475, 558, 700
Tth111I GACNNNGTC 1 cut(s) 512
VpaK11BI GGWCC 4 cut(s) 441, 503, 571, 643
XapI RAATTY 2 cut(s) 44, 707
XceI RCATGY 1 cut(s) 209
XspI CTAG 1 cut(s) 224
ZraI GACGTC 1 cut(s) 814
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.