MD09G1161000.v1.1

Pathogenesis-related genes transcriptional activator PTI6-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0009231
Physical Location & Seq
Forward (+)
12985072 .. 12985842
771 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1161000.v1.1.491

Sequence Viewer

Length: 771 bp
ATGTCAGATTCTAATTCCCCCTCACCCGCTTGTCTTTTGCTTTTTTACTATTTGAGCTTTTTCCCCCCTTCGATTCTTCATCTCTCTCACCACCAAATCCCTAATTCAATTCCGCCGCCATCAAATTCCATGTCGACCGTCCAAATCAACCGCTCCGCCGCCAATTCCGATGTCCGATTTTCGAAGCAAACAACCGCCCTAGCCAAACCCGTGATCCGAGAAACCCCAAACCCACCCCGAAACCCGAGAATCCAACCAAAGCTTGTGCGGATCATACACGCCGACGCCGACGCAACCGACTCCTCCAGCGACGACAACGAGGACAGCGTCGTGCCGACGGTGAAGCGTCGCGTCAGAGAGATCAACCTTGAGGTCTCTTCGCCTTCCTCTGCTTCGTCTTCTCCGCCTTGCTCCGGTCGTCCTAAGCTGTCCAAACGAGCCAAGCTGCAACCGAAATCGGGCCCGGACCGCCGGAAGGGGTTCGTCGGCATCCGGCAGAGACCATGGGGCAAGTGGGCTGCGGAGATTCGAGACCCGAATCAGGGAAAACGGCTTTGGCTCGGAACCTTCGACACGGCGGAGGAAGCTGCGGCGGCGTACGACAGAGCTTCGCTGATGCTCAGGGGCCCTCGTGCAGTCACTAACTTTCCTGGAGCTGGGGAGACGGTGGTCGCTGATGAGGTGGTCGCCGCTGCGGAAGTGGGCCATGGGGAAGGAGGAGGAGCAGTCGCCGCCGTGGGTGGAGGAGAGGGGGCCGCAGATTGTCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
AP2

Protein Analysis

257

Amino Acids

27.28

Weight (kDa)

9.24

Isoelectric Point (pI)

55.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 160 - 209 1.2e-14 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 153
AccI GTMKAC 1 cut(s) 134
AccII CGCG 1 cut(s) 351
AclWI GGATC 2 cut(s) 208, 278
AcsI RAATTY 1 cut(s) 124
AcyI GRCGYC 1 cut(s) 285
AfaI GTAC 1 cut(s) 599
AfiI CCNNNNNNNGG 6 cut(s) 413, 458, 475, 541, 542, 656
AgsI TTSAA 1 cut(s) 108
AjnI CCWGG 1 cut(s) 649
AjuI GAANNNNNNNTTGG 2 cut(s) 538, 570
AluBI AGCT 7 cut(s) 57, 262, 427, 445, 587, 608, 656
AluI AGCT 7 cut(s) 57, 262, 427, 445, 587, 608, 656
Alw26I GTCTC 4 cut(s) 379, 493, 525, 656
AlwI GGATC 2 cut(s) 208, 278
Ama87I CYCGRG 1 cut(s) 244
AoxI GGCC 4 cut(s) 460, 625, 703, 753
ApaI GGGCCC 2 cut(s) 464, 629
ApeKI GCWGC 4 cut(s) 445, 518, 587, 692
ApoI RAATTY 1 cut(s) 124
ArsI GACNNNNNNTTYG 2 cut(s) 116, 148
Asp700I GAANNNNTTC 1 cut(s) 479
AspS9I GGNCC 7 cut(s) 460, 461, 466, 625, 626, 703, 753
AsuC2I CCSGG 1 cut(s) 464
AsuHPI GGTGA 3 cut(s) 15, 80, 352
AsuII TTCGAA 1 cut(s) 182
AvaI CYCGRG 1 cut(s) 244
AvaII GGWCC 1 cut(s) 466
BaeGI GKGCMC 2 cut(s) 464, 629
BanII GRGCYC 2 cut(s) 464, 629
BauI CACGAG 1 cut(s) 630
BbsI GAAGAC 1 cut(s) 390
BbvI GCAGC 4 cut(s) 432, 505, 574, 679
BccI CCATC 1 cut(s) 127
BceAI ACGGC 3 cut(s) 566, 591, 719
BciT130I CCWGG 1 cut(s) 651
BcnI CCSGG 1 cut(s) 464
BcoDI GTCTC 4 cut(s) 379, 493, 525, 656
BfaI CTAG 1 cut(s) 200
Bme1390I CCNGG 2 cut(s) 464, 651
Bme18I GGWCC 1 cut(s) 466
BmeT110I CYCGRG 1 cut(s) 244
BmgT120I GGNCC 7 cut(s) 460, 461, 466, 625, 626, 703, 753
BmiI GGNNCC 5 cut(s) 462, 565, 626, 627, 754
BmrFI CCNGG 2 cut(s) 464, 651
BmsI GCATC 2 cut(s) 498, 606
BoxI GACNNNNGTC 1 cut(s) 668
BpiI GAAGAC 1 cut(s) 390
BpmI CTGGAG 2 cut(s) 289, 672
Bpu10I CCTNAGC 2 cut(s) 423, 620
Bpu14I TTCGAA 1 cut(s) 182
BpuEI CTTGAG 1 cut(s) 389
BpuMI CCSGG 1 cut(s) 464
BsaHI GRCGYC 1 cut(s) 285
BsaI GGTCTC 3 cut(s) 379, 493, 525
BsaJI CCNNGG 3 cut(s) 503, 706, 735
BsaWI WCCGGW 1 cut(s) 413
BsaXI ACNNNNNCTCC 2 cut(s) 711, 741
Bsc4I CCNNNNNNNGG 6 cut(s) 413, 458, 475, 541, 542, 656
BseBI CCWGG 1 cut(s) 651
BseDI CCNNGG 3 cut(s) 503, 706, 735
BseGI GGATG 1 cut(s) 489
BseLI CCNNNNNNNGG 6 cut(s) 413, 458, 475, 541, 542, 656
BseMII CTCAG 1 cut(s) 634
BseRI GAGGAG 4 cut(s) 292, 732, 735, 759
BseSI GKGCMC 2 cut(s) 464, 629
BseXI GCAGC 4 cut(s) 432, 505, 574, 679
BseYI CCCAGC 1 cut(s) 656
BsgI GTGCAG 1 cut(s) 654
Bsh1236I CGCG 1 cut(s) 351
Bsh1285I CGRYCG 2 cut(s) 138, 418
BshFI GGCC 4 cut(s) 462, 627, 705, 755
BsiEI CGRYCG 2 cut(s) 138, 418
BsiHKCI CYCGRG 1 cut(s) 244
BsiSI CCGG 4 cut(s) 414, 464, 472, 493
BsiWI CGTACG 1 cut(s) 597
BslI CCNNNNNNNGG 6 cut(s) 413, 458, 475, 541, 542, 656
BsmAI GTCTC 4 cut(s) 379, 493, 525, 656
BsmBI CGTCTC 1 cut(s) 656
BsnI GGCC 4 cut(s) 462, 627, 705, 755
Bso31I GGTCTC 3 cut(s) 379, 493, 525
BsoBI CYCGRG 1 cut(s) 244
Bsp119I TTCGAA 1 cut(s) 182
Bsp120I GGGCCC 2 cut(s) 460, 625
Bsp1286I GDGCHC 2 cut(s) 464, 629
Bsp143I GATC 3 cut(s) 213, 270, 360
Bsp19I CCATGG 2 cut(s) 503, 706
BspANI GGCC 4 cut(s) 462, 627, 705, 755
BspCNI CTCAG 1 cut(s) 633
BspFNI CGCG 1 cut(s) 351
BspLI GGNNCC 5 cut(s) 462, 565, 626, 627, 754
BspPI GGATC 2 cut(s) 208, 278
BspT104I TTCGAA 1 cut(s) 182
BspTNI GGTCTC 3 cut(s) 379, 493, 525
BsrBI CCGCTC 1 cut(s) 153
BssECI CCNNGG 3 cut(s) 503, 706, 735
BssMI GATC 3 cut(s) 213, 270, 360
BssNI GRCGYC 1 cut(s) 285
BssSI CACGAG 1 cut(s) 630
BssT1I CCWWGG 2 cut(s) 503, 706
Bst2BI CACGAG 1 cut(s) 630
Bst2UI CCWGG 1 cut(s) 651
Bst4CI ACNGT 3 cut(s) 139, 340, 667
Bst6I CTCTTC 1 cut(s) 382
BstACI GRCGYC 1 cut(s) 285
BstBI TTCGAA 1 cut(s) 182
BstDEI CTNAG 2 cut(s) 423, 620
BstDSI CCRYGG 3 cut(s) 503, 706, 735
BstF5I GGATG 1 cut(s) 489
BstFNI CGCG 1 cut(s) 351
BstKTI GATC 3 cut(s) 216, 273, 363
BstMAI GTCTC 4 cut(s) 379, 493, 525, 656
BstMBI GATC 3 cut(s) 213, 270, 360
BstMCI CGRYCG 2 cut(s) 138, 418
BstMWI GCNNNNNNNGC 4 cut(s) 468, 584, 593, 731
BstNI CCWGG 1 cut(s) 651
BstPAI GACNNNNGTC 1 cut(s) 668
BstSCI CCNGG 2 cut(s) 462, 649
BstSLI GKGCMC 2 cut(s) 464, 629
BstUI CGCG 1 cut(s) 351
BstV1I GCAGC 4 cut(s) 432, 505, 574, 679
BstV2I GAAGAC 1 cut(s) 390
BsuRI GGCC 4 cut(s) 462, 627, 705, 755
BtgI CCRYGG 3 cut(s) 503, 706, 735
BtsCI GGATG 1 cut(s) 489
Cfr13I GGNCC 7 cut(s) 460, 461, 466, 625, 626, 703, 753
CpoI CGGWCCG 1 cut(s) 466
CseI GACGC 5 cut(s) 293, 299, 316, 335, 340
Csp6I GTAC 1 cut(s) 598
CspI CGGWCCG 1 cut(s) 466
CviAII CATG 3 cut(s) 130, 504, 707
CviQI GTAC 1 cut(s) 598
DdeI CTNAG 2 cut(s) 423, 620
DpnI GATC 3 cut(s) 215, 272, 362
DpnII GATC 3 cut(s) 213, 270, 360
Eam1104I CTCTTC 1 cut(s) 382
EarI CTCTTC 1 cut(s) 382
EciI GGCGGA 4 cut(s) 102, 145, 393, 593
Eco130I CCWWGG 2 cut(s) 503, 706
Eco24I GRGCYC 2 cut(s) 464, 629
Eco31I GGTCTC 3 cut(s) 379, 493, 525
Eco47I GGWCC 1 cut(s) 466
Eco88I CYCGRG 1 cut(s) 244
EcoO109I RGGNCCY 2 cut(s) 625, 626
EcoRII CCWGG 1 cut(s) 649
EcoT14I CCWWGG 2 cut(s) 503, 706
EcoT38I GRGCYC 2 cut(s) 464, 629
ErhI CCWWGG 2 cut(s) 503, 706
Esp3I CGTCTC 1 cut(s) 656
FaeI CATG 3 cut(s) 133, 507, 710
FaiI YATR 4 cut(s) 131, 275, 505, 708
FatI CATG 3 cut(s) 129, 503, 706
FauI CCCGC 1 cut(s) 34
FblI GTMKAC 1 cut(s) 134
FokI GGATG 1 cut(s) 476
FriOI GRGCYC 2 cut(s) 464, 629
FspBI CTAG 1 cut(s) 200
GsaI CCCAGC 1 cut(s) 660
GsuI CTGGAG 2 cut(s) 289, 672
HaeIII GGCC 4 cut(s) 462, 627, 705, 755
HapII CCGG 4 cut(s) 414, 464, 472, 493
HgaI GACGC 5 cut(s) 293, 299, 316, 335, 340
Hin1I GRCGYC 1 cut(s) 285
Hin1II CATG 3 cut(s) 133, 507, 710
HincII GTYRAC 1 cut(s) 135
HindII GTYRAC 1 cut(s) 135
HindIII AAGCTT 1 cut(s) 260
HinfI GANTC 6 cut(s) 8, 73, 249, 299, 526, 538
HpaII CCGG 4 cut(s) 414, 464, 472, 493
HphI GGTGA 3 cut(s) 15, 80, 352
Hpy166II GTNNAC 1 cut(s) 135
Hpy188I TCNGA 6 cut(s) 7, 169, 176, 218, 356, 563
Hpy188III TCNNGA 1 cut(s) 530
Hpy8I GTNNAC 1 cut(s) 135
Hpy99I CGWCG 7 cut(s) 287, 293, 314, 332, 340, 351, 488
HpyAV CCTTC 5 cut(s) 78, 393, 469, 577, 707
HpyCH4III ACNGT 3 cut(s) 139, 340, 667
HpyCH4V TGCA 2 cut(s) 448, 635
HpyF10VI GCNNNNNNNGC 4 cut(s) 468, 584, 593, 731
HpyF3I CTNAG 2 cut(s) 423, 620
Hsp92I GRCGYC 1 cut(s) 285
Hsp92II CATG 3 cut(s) 133, 507, 710
Kzo9I GATC 3 cut(s) 213, 270, 360
LmnI GCTCC 4 cut(s) 158, 416, 653, 722
Lsp1109I GCAGC 4 cut(s) 432, 505, 574, 679
LweI GCATC 2 cut(s) 498, 606
MaeI CTAG 1 cut(s) 200
MaeIII GTNAC 1 cut(s) 637
MalI GATC 3 cut(s) 215, 272, 362
MbiI CCGCTC 1 cut(s) 153
MboI GATC 3 cut(s) 213, 270, 360
MboII GAAGA 3 cut(s) 68, 369, 390
MhlI GDGCHC 2 cut(s) 464, 629
MluCI AATT 5 cut(s) 13, 103, 108, 124, 163
MlyI GAGTC 1 cut(s) 293
MmeI TCCRAC 1 cut(s) 277
MroXI GAANNNNTTC 1 cut(s) 479
MseI TTAA 1 cut(s) 769
MspA1I CMGCKG 1 cut(s) 692
MspI CCGG 4 cut(s) 414, 464, 472, 493
MspR9I CCNGG 2 cut(s) 464, 651
MvaI CCWGG 1 cut(s) 651
MvnI CGCG 1 cut(s) 351
MwoI GCNNNNNNNGC 4 cut(s) 468, 584, 593, 731
NciI CCSGG 1 cut(s) 464
NcoI CCATGG 2 cut(s) 503, 706
NdeII GATC 3 cut(s) 213, 270, 360
NlaIII CATG 3 cut(s) 133, 507, 710
NlaIV GGNNCC 5 cut(s) 462, 565, 626, 627, 754
NmuCI GTSAC 1 cut(s) 637
NspV TTCGAA 1 cut(s) 182
PcsI WCGNNNNNNNCGW 3 cut(s) 285, 324, 567
PdmI GAANNNNTTC 1 cut(s) 479
PfeI GAWTC 5 cut(s) 8, 73, 249, 526, 538
Pfl23II CGTACG 1 cut(s) 597
PflFI GACNNNGTC 1 cut(s) 326
PfoI TCCNGGA 1 cut(s) 649
PleI GAGTC 1 cut(s) 293
PpsI GAGTC 1 cut(s) 293
PshAI GACNNNNGTC 1 cut(s) 668
Psp6I CCWGG 1 cut(s) 649
PspFI CCCAGC 1 cut(s) 656
PspGI CCWGG 1 cut(s) 649
PspLI CGTACG 1 cut(s) 597
PspN4I GGNNCC 5 cut(s) 462, 565, 626, 627, 754
PspOMI GGGCCC 2 cut(s) 460, 625
PspPI GGNCC 7 cut(s) 460, 461, 466, 625, 626, 703, 753
PsyI GACNNNGTC 1 cut(s) 326
RsaI GTAC 1 cut(s) 599
RsaNI GTAC 1 cut(s) 598
Rsr2I CGGWCCG 1 cut(s) 466
RsrII CGGWCCG 1 cut(s) 466
SalI GTCGAC 1 cut(s) 133
SaqAI TTAA 1 cut(s) 769
Sau3AI GATC 3 cut(s) 213, 270, 360
Sau96I GGNCC 7 cut(s) 460, 461, 466, 625, 626, 703, 753
SchI GAGTC 1 cut(s) 293
ScrFI CCNGG 2 cut(s) 464, 651
SduI GDGCHC 2 cut(s) 464, 629
SfaNI GCATC 2 cut(s) 498, 606
SfuI TTCGAA 1 cut(s) 182
SinI GGWCC 1 cut(s) 466
SmlI CTYRAG 1 cut(s) 368
SmoI CTYRAG 1 cut(s) 368
Sse9I AATT 5 cut(s) 13, 103, 108, 124, 163
SspMI CTAG 1 cut(s) 200
StyD4I CCNGG 2 cut(s) 462, 649
StyI CCWWGG 2 cut(s) 503, 706
TaaI ACNGT 3 cut(s) 139, 340, 667
TaqI TCGA 5 cut(s) 71, 134, 182, 529, 570
TasI AATT 5 cut(s) 13, 103, 108, 124, 163
TauI GCSGC 7 cut(s) 118, 161, 593, 596, 692, 734, 758
TfiI GAWTC 5 cut(s) 8, 73, 249, 526, 538
Tru1I TTAA 1 cut(s) 769
Tru9I TTAA 1 cut(s) 769
TseFI GTSAC 1 cut(s) 637
TseI GCWGC 4 cut(s) 445, 518, 587, 692
Tsp45I GTSAC 1 cut(s) 637
TspDTI ATGAA 1 cut(s) 68
Tth111I GACNNNGTC 1 cut(s) 326
VpaK11BI GGWCC 1 cut(s) 466
XapI RAATTY 1 cut(s) 124
XcmI CCANNNNNNNNNTGG 1 cut(s) 510
XmiI GTMKAC 1 cut(s) 134
XmnI GAANNNNTTC 1 cut(s) 479
XspI CTAG 1 cut(s) 200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.