pycom03g03950

Pathogenesis-related genes transcriptional activator PTI6-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0009231
Physical Location & Seq
Forward (+)
3122719 .. 3123543
825 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g03950.1

Sequence Viewer

Length: 825 bp
ATGACAACCTTCCAACCCATCTCGTCCGTACACTCAACGGTCAAATTTTCAGAGCAAATAACCACCGTGATAAAACCCATGATCCAGGAACCCGATAACCCGACTCGTATCCCGCCAAAGCTCGTCCGGATCATACTTACGGATGCCGACGCCACCGACTCCTCCAGCGACGACGAAGGCAGAAGCGTGCGCAGAGTCAAACGACATGTTAGACAGATCAGCCTAGAGCACTCCTCGCCTTCCTCTTCCTCTCCTTCATCTTCGTCGTCGGTTTCTTCGGGTCAGTCGAAGCGGGCGAGGAAAGACTCGCGACCCGGATCGAAGTCACCGGAAACGGCCGCGGATCTCCTGAAAAAGTTTAGAGGCGTCCGGCGGAGGCCTTGGGGTCGCTGGGCCGCGGAGATCCGAGACCCGACCCGAAGAAAACGGATCTGGCTCGGGACCTTCGACACGGCGGAGGAAGCCGCCACAGTTTACGACAGAGCTGCCATCTTGCTCAAGGGTCCTGACGCCGTCACCAACTTTTCTAACGCCGTTATAAAGCAGGAAACAGTCGTTGGCGATTTGACGGTGCCAGCTGCGGAGCAGGGATGTGGGTGTGTAACTCCGTCGTCCAGCGGGGCTCTCTCGTCGCCTACTTCGGTCCTCGGTTACGGTGACCAAACGCCATTTGATGGTTTAAGTTACGGATACGCCGACGCCTCCGAATTTGATTTCGGCTTGCCGTTAAGCTTGCCGGATATCATGTTGTCAAGTAACAGCTTTGCACGCGACGAGTTCGGCGAGTTTGACATAGACGATTTCTTGGCTGACGTCGTATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
AP2

Protein Analysis

275

Amino Acids

29.75

Weight (kDa)

5.73

Isoelectric Point (pI)

58.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 119 - 168 5.7e-15 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 539
AatII GACGTC 1 cut(s) 816
Acc16I TGCGCA 1 cut(s) 191
AccB1I GGYRCC 1 cut(s) 571
AccB7I CCANNNNNTGG 1 cut(s) 674
AccII CGCG 4 cut(s) 310, 341, 398, 771
AccIII TCCGGA 1 cut(s) 126
AclWI GGATC 6 cut(s) 76, 137, 325, 351, 397, 437
AcoI YGGCCR 1 cut(s) 336
AcsI RAATTY 2 cut(s) 44, 707
AcyI GRCGYC 5 cut(s) 150, 366, 510, 699, 813
AfaI GTAC 1 cut(s) 30
AfiI CCNNNNNNNGG 1 cut(s) 674
AflIII ACRYGT 1 cut(s) 205
AjnI CCWGG 1 cut(s) 84
AjuI GAANNNNNNNTTGG 2 cut(s) 540, 572
AluBI AGCT 5 cut(s) 121, 485, 578, 732, 762
AluI AGCT 5 cut(s) 121, 485, 578, 732, 762
Alw21I GWGCWC 1 cut(s) 231
Alw26I GTCTC 1 cut(s) 402
AlwI GGATC 6 cut(s) 76, 137, 325, 351, 397, 437
Ama87I CYCGRG 1 cut(s) 437
Aor13HI TCCGGA 1 cut(s) 126
AoxI GGCC 3 cut(s) 336, 377, 393
ApeKI GCWGC 2 cut(s) 485, 578
ApoI RAATTY 2 cut(s) 44, 707
AspLEI GCGC 1 cut(s) 192
AspS9I GGNCC 4 cut(s) 393, 441, 503, 643
AsuC2I CCSGG 1 cut(s) 315
AsuHPI GGTGA 3 cut(s) 318, 508, 668
AvaI CYCGRG 1 cut(s) 437
AvaII GGWCC 3 cut(s) 441, 503, 643
BanI GGYRCC 1 cut(s) 571
BanII GRGCYC 1 cut(s) 625
Bbv12I GWGCWC 1 cut(s) 231
BbvI GCAGC 2 cut(s) 472, 565
BccI CCATC 3 cut(s) 26, 497, 668
BceAI ACGGC 5 cut(s) 351, 468, 497, 518, 709
BcgI CGANNNNNNTGC 2 cut(s) 467, 501
BciT130I CCWGG 1 cut(s) 86
BciVI GTATCC 2 cut(s) 119, 683
BcnI CCSGG 1 cut(s) 315
BcoDI GTCTC 1 cut(s) 402
BfaI CTAG 1 cut(s) 224
BfuI GTATCC 2 cut(s) 119, 683
BisI GCNGC 5 cut(s) 339, 396, 465, 486, 579
BlsI GCNGC 5 cut(s) 340, 397, 466, 487, 580
Bme1390I CCNGG 2 cut(s) 86, 315
Bme18I GGWCC 3 cut(s) 441, 503, 643
BmeT110I CYCGRG 1 cut(s) 437
BmgT120I GGNCC 4 cut(s) 393, 441, 503, 643
BmiI GGNNCC 4 cut(s) 90, 442, 504, 573
BmrFI CCNGG 2 cut(s) 86, 315
BmsI GCATC 1 cut(s) 133
BplI GAGNNNNNCTC 2 cut(s) 218, 250
BpmI CTGGAG 1 cut(s) 148
BpuEI CTTGAG 1 cut(s) 482
BpuMI CCSGG 1 cut(s) 315
BsaHI GRCGYC 5 cut(s) 150, 366, 510, 699, 813
BsaI GGTCTC 1 cut(s) 402
BsaJI CCNNGG 4 cut(s) 339, 380, 396, 646
BsaWI WCCGGW 2 cut(s) 126, 328
Bsc4I CCNNNNNNNGG 1 cut(s) 674
BseAI TCCGGA 1 cut(s) 126
BseBI CCWGG 1 cut(s) 86
BseDI CCNNGG 4 cut(s) 339, 380, 396, 646
BseGI GGATG 2 cut(s) 148, 596
BseLI CCNNNNNNNGG 1 cut(s) 674
BseRI GAGGAG 2 cut(s) 151, 223
BseX3I CGGCCG 1 cut(s) 336
BseXI GCAGC 2 cut(s) 472, 565
BseYI CCCAGC 1 cut(s) 390
Bsh1236I CGCG 4 cut(s) 310, 341, 398, 771
Bsh1285I CGRYCG 1 cut(s) 339
BshFI GGCC 3 cut(s) 338, 379, 395
BshNI GGYRCC 1 cut(s) 571
BsiEI CGRYCG 1 cut(s) 339
BsiHKAI GWGCWC 1 cut(s) 231
BsiHKCI CYCGRG 1 cut(s) 437
BsiSI CCGG 5 cut(s) 127, 315, 329, 370, 737
BslFI GGGAC 1 cut(s) 454
BslI CCNNNNNNNGG 1 cut(s) 674
BsmAI GTCTC 1 cut(s) 402
BsmFI GGGAC 1 cut(s) 454
BsnI GGCC 3 cut(s) 338, 379, 395
Bso31I GGTCTC 1 cut(s) 402
BsoBI CYCGRG 1 cut(s) 437
Bsp1286I GDGCHC 2 cut(s) 231, 625
Bsp13I TCCGGA 1 cut(s) 126
Bsp143I GATC 7 cut(s) 81, 129, 216, 317, 343, 402, 429
Bsp68I TCGCGA 1 cut(s) 310
BspANI GGCC 3 cut(s) 338, 379, 395
BspEI TCCGGA 1 cut(s) 126
BspFNI CGCG 4 cut(s) 310, 341, 398, 771
BspLI GGNNCC 4 cut(s) 90, 442, 504, 573
BspPI GGATC 6 cut(s) 76, 137, 325, 351, 397, 437
BspT107I GGYRCC 1 cut(s) 571
BspTNI GGTCTC 1 cut(s) 402
BssECI CCNNGG 4 cut(s) 339, 380, 396, 646
BssMI GATC 7 cut(s) 81, 129, 216, 317, 343, 402, 429
BssNI GRCGYC 5 cut(s) 150, 366, 510, 699, 813
BssT1I CCWWGG 1 cut(s) 380
Bst2UI CCWGG 1 cut(s) 86
Bst4CI ACNGT 6 cut(s) 40, 67, 472, 553, 571, 656
Bst6I CTCTTC 1 cut(s) 250
BstACI GRCGYC 5 cut(s) 150, 366, 510, 699, 813
BstC8I GCNNGC 6 cut(s) 188, 294, 576, 722, 734, 769
BstDSI CCRYGG 2 cut(s) 339, 396
BstEII GGTNACC 1 cut(s) 656
BstF5I GGATG 2 cut(s) 148, 596
BstFNI CGCG 4 cut(s) 310, 341, 398, 771
BstHHI GCGC 1 cut(s) 192
BstKTI GATC 7 cut(s) 84, 132, 219, 320, 346, 405, 432
BstMAI GTCTC 1 cut(s) 402
BstMBI GATC 7 cut(s) 81, 129, 216, 317, 343, 402, 429
BstMCI CGRYCG 1 cut(s) 339
BstMWI GCNNNNNNNGC 3 cut(s) 235, 461, 768
BstNI CCWGG 1 cut(s) 86
BstNSI RCATGY 1 cut(s) 209
BstPI GGTNACC 1 cut(s) 656
BstSCI CCNGG 2 cut(s) 84, 313
BstUI CGCG 4 cut(s) 310, 341, 398, 771
BstV1I GCAGC 2 cut(s) 472, 565
BstX2I RGATCY 3 cut(s) 343, 402, 429
BstYI RGATCY 3 cut(s) 343, 402, 429
BstZI CGGCCG 1 cut(s) 336
BsuI GTATCC 2 cut(s) 119, 683
BsuRI GGCC 3 cut(s) 338, 379, 395
BtgI CCRYGG 2 cut(s) 339, 396
BtsCI GGATG 2 cut(s) 148, 596
BtuMI TCGCGA 1 cut(s) 310
Cac8I GCNNGC 6 cut(s) 188, 294, 576, 722, 734, 769
CfoI GCGC 1 cut(s) 192
Cfr13I GGNCC 4 cut(s) 393, 441, 503, 643
Cfr42I CCGCGG 2 cut(s) 342, 399
CseI GACGC 4 cut(s) 158, 355, 518, 707
Csp6I GTAC 1 cut(s) 29
CviAII CATG 3 cut(s) 79, 206, 745
CviQI GTAC 1 cut(s) 29
DpnI GATC 7 cut(s) 83, 131, 218, 319, 345, 404, 431
DpnII GATC 7 cut(s) 81, 129, 216, 317, 343, 402, 429
EaeI YGGCCR 1 cut(s) 336
EagI CGGCCG 1 cut(s) 336
Eam1104I CTCTTC 1 cut(s) 250
EarI CTCTTC 1 cut(s) 250
EciI GGCGGA 2 cut(s) 388, 470
EclXI CGGCCG 1 cut(s) 336
Eco130I CCWWGG 1 cut(s) 380
Eco147I AGGCCT 1 cut(s) 379
Eco24I GRGCYC 1 cut(s) 625
Eco31I GGTCTC 1 cut(s) 402
Eco32I GATATC 1 cut(s) 742
Eco47I GGWCC 3 cut(s) 441, 503, 643
Eco52I CGGCCG 1 cut(s) 336
Eco88I CYCGRG 1 cut(s) 437
Eco91I GGTNACC 1 cut(s) 656
EcoO109I RGGNCCY 2 cut(s) 441, 503
EcoO65I GGTNACC 1 cut(s) 656
EcoRII CCWGG 1 cut(s) 84
EcoRV GATATC 1 cut(s) 742
EcoT14I CCWWGG 1 cut(s) 380
EcoT38I GRGCYC 1 cut(s) 625
ErhI CCWWGG 1 cut(s) 380
FaeI CATG 3 cut(s) 82, 209, 748
FaiI YATR 7 cut(s) 80, 134, 207, 539, 746, 794, 820
FaqI GGGAC 1 cut(s) 454
FatI CATG 3 cut(s) 78, 205, 744
FauI CCCGC 3 cut(s) 120, 285, 611
Fnu4HI GCNGC 5 cut(s) 339, 396, 465, 486, 579
FokI GGATG 2 cut(s) 155, 603
FriOI GRGCYC 1 cut(s) 625
Fsp4HI GCNGC 5 cut(s) 339, 396, 465, 486, 579
FspBI CTAG 1 cut(s) 224
FspI TGCGCA 1 cut(s) 191
GlaI GCGC 1 cut(s) 191
GluI GCNGC 5 cut(s) 339, 396, 465, 486, 579
GsaI CCCAGC 1 cut(s) 394
GsuI CTGGAG 1 cut(s) 148
HaeIII GGCC 3 cut(s) 338, 379, 395
HapII CCGG 5 cut(s) 127, 315, 329, 370, 737
HgaI GACGC 4 cut(s) 158, 355, 518, 707
HhaI GCGC 1 cut(s) 192
Hin1I GRCGYC 5 cut(s) 150, 366, 510, 699, 813
Hin1II CATG 3 cut(s) 82, 209, 748
Hin6I GCGC 1 cut(s) 190
HinP1I GCGC 1 cut(s) 190
HindIII AAGCTT 1 cut(s) 730
HinfI GANTC 4 cut(s) 103, 158, 195, 305
HpaII CCGG 5 cut(s) 127, 315, 329, 370, 737
HphI GGTGA 3 cut(s) 318, 508, 668
Hpy166II GTNNAC 2 cut(s) 31, 475
Hpy188I TCNGA 3 cut(s) 52, 407, 706
Hpy188III TCNNGA 5 cut(s) 127, 309, 349, 439, 506
Hpy8I GTNNAC 2 cut(s) 31, 475
HpyAV CCTTC 5 cut(s) 19, 170, 249, 264, 454
HpyCH4III ACNGT 6 cut(s) 40, 67, 472, 553, 571, 656
HpyCH4IV ACGT 1 cut(s) 813
HpyCH4V TGCA 1 cut(s) 767
HpyF10VI GCNNNNNNNGC 3 cut(s) 235, 461, 768
HpySE526I ACGT 1 cut(s) 813
Hsp92I GRCGYC 5 cut(s) 150, 366, 510, 699, 813
Hsp92II CATG 3 cut(s) 82, 209, 748
HspAI GCGC 1 cut(s) 190
Kpn2I TCCGGA 1 cut(s) 126
KspI CCGCGG 2 cut(s) 342, 399
Kzo9I GATC 7 cut(s) 81, 129, 216, 317, 343, 402, 429
LmnI GCTCC 1 cut(s) 583
Lsp1109I GCAGC 2 cut(s) 472, 565
LweI GCATC 1 cut(s) 133
MaeI CTAG 1 cut(s) 224
MaeII ACGT 1 cut(s) 813
MaeIII GTNAC 7 cut(s) 324, 514, 601, 650, 656, 683, 755
MalI GATC 7 cut(s) 83, 131, 218, 319, 345, 404, 431
MboI GATC 7 cut(s) 81, 129, 216, 317, 343, 402, 429
MboII GAAGA 4 cut(s) 237, 252, 267, 432
MflI RGATCY 3 cut(s) 343, 402, 429
MhlI GDGCHC 2 cut(s) 231, 625
MluCI AATT 2 cut(s) 44, 707
MlyI GAGTC 4 cut(s) 97, 152, 204, 299
MmeI TCCRAC 1 cut(s) 37
MroI TCCGGA 1 cut(s) 126
MseI TTAA 2 cut(s) 680, 728
MspA1I CMGCKG 4 cut(s) 341, 398, 578, 618
MspI CCGG 5 cut(s) 127, 315, 329, 370, 737
MspR9I CCNGG 2 cut(s) 86, 315
MvaI CCWGG 1 cut(s) 86
MvnI CGCG 4 cut(s) 310, 341, 398, 771
MwoI GCNNNNNNNGC 3 cut(s) 235, 461, 768
NciI CCSGG 1 cut(s) 315
NdeII GATC 7 cut(s) 81, 129, 216, 317, 343, 402, 429
NlaIII CATG 3 cut(s) 82, 209, 748
NlaIV GGNNCC 4 cut(s) 90, 442, 504, 573
NmuCI GTSAC 3 cut(s) 324, 514, 656
NruI TCGCGA 1 cut(s) 310
NsbI TGCGCA 1 cut(s) 191
NspI RCATGY 1 cut(s) 209
PceI AGGCCT 1 cut(s) 379
PciI ACATGT 1 cut(s) 205
PcsI WCGNNNNNNNCGW 5 cut(s) 284, 293, 444, 693, 780
PflFI GACNNNGTC 1 cut(s) 512
PflMI CCANNNNNTGG 1 cut(s) 674
PfoI TCCNGGA 1 cut(s) 84
PkrI GCNGC 5 cut(s) 340, 397, 466, 487, 580
PleI GAGTC 4 cut(s) 97, 152, 203, 299
PpsI GAGTC 4 cut(s) 97, 152, 203, 299
PpuMI RGGWCCY 2 cut(s) 441, 503
PscI ACATGT 1 cut(s) 205
PsiI TTATAA 1 cut(s) 539
Psp5II RGGWCCY 2 cut(s) 441, 503
Psp6I CCWGG 1 cut(s) 84
PspEI GGTNACC 1 cut(s) 656
PspFI CCCAGC 1 cut(s) 390
PspGI CCWGG 1 cut(s) 84
PspN4I GGNNCC 4 cut(s) 90, 442, 504, 573
PspPI GGNCC 4 cut(s) 393, 441, 503, 643
PspPPI RGGWCCY 2 cut(s) 441, 503
PsuI RGATCY 3 cut(s) 343, 402, 429
PsyI GACNNNGTC 1 cut(s) 512
PvuII CAGCTG 1 cut(s) 578
RruI TCGCGA 1 cut(s) 310
RsaI GTAC 1 cut(s) 30
RsaNI GTAC 1 cut(s) 29
SacII CCGCGG 2 cut(s) 342, 399
SaqAI TTAA 2 cut(s) 680, 728
SatI GCNGC 5 cut(s) 339, 396, 465, 486, 579
Sau3AI GATC 7 cut(s) 81, 129, 216, 317, 343, 402, 429
Sau96I GGNCC 4 cut(s) 393, 441, 503, 643
SchI GAGTC 4 cut(s) 97, 152, 204, 299
ScrFI CCNGG 2 cut(s) 86, 315
SduI GDGCHC 2 cut(s) 231, 625
SetI ASST 8 cut(s) 11, 123, 446, 487, 580, 734, 764, 816
SfaNI GCATC 1 cut(s) 133
Sfr303I CCGCGG 2 cut(s) 342, 399
SgrBI CCGCGG 2 cut(s) 342, 399
SinI GGWCC 3 cut(s) 441, 503, 643
SmlI CTYRAG 1 cut(s) 497
SmoI CTYRAG 1 cut(s) 497
Sse9I AATT 2 cut(s) 44, 707
SseBI AGGCCT 1 cut(s) 379
SspMI CTAG 1 cut(s) 224
StuI AGGCCT 1 cut(s) 379
StyD4I CCNGG 2 cut(s) 84, 313
StyI CCWWGG 1 cut(s) 380
TaaI ACNGT 6 cut(s) 40, 67, 472, 553, 571, 656
TaiI ACGT 1 cut(s) 816
TaqI TCGA 3 cut(s) 287, 320, 447
TaqII GACCGA 1 cut(s) 631
TasI AATT 2 cut(s) 44, 707
TauI GCSGC 3 cut(s) 341, 398, 467
Tru1I TTAA 2 cut(s) 680, 728
Tru9I TTAA 2 cut(s) 680, 728
TseFI GTSAC 3 cut(s) 324, 514, 656
TseI GCWGC 2 cut(s) 485, 578
Tsp45I GTSAC 3 cut(s) 324, 514, 656
TspDTI ATGAA 1 cut(s) 246
TspGWI ACGGA 5 cut(s) 16, 155, 442, 597, 702
Tth111I GACNNNGTC 1 cut(s) 512
Van91I CCANNNNNTGG 1 cut(s) 674
VpaK11BI GGWCC 3 cut(s) 441, 503, 643
XapI RAATTY 2 cut(s) 44, 707
XceI RCATGY 1 cut(s) 209
XspI CTAG 1 cut(s) 224
ZraI GACGTC 1 cut(s) 814
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.