pycom11g04250

Pathogenesis-related genes transcriptional activator PTI6-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0009231
Physical Location & Seq
Forward (+)
3297296 .. 3298213
918 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g04250.1

Sequence Viewer

Length: 918 bp
ATGCTTTCGCCGTCGATTCTTGATGTTGCTGCTTCCGACCAAACCCGCATCTCCAATTTCATTTCCTCCGCCTCCAAATCCATGAAAACCTTCCAACCCACCTCGTCCGCACACTCTGCGGTCAAATTTTCAGAGCAAATCACCACCGTGACAAAACCCATGATCCAGGAACCCGATAACCCGAACCCAGTCCCGCCAAAGCTCGTCCGGATCATACTCACGGACGCCGACGCCACCGACTCCTCCAGCGACGACGAAGACAGCGGCGTACGCAGAGTCAAACGACATGTCAGACAGATCAGCCTCGAGCTCTCCTCGCCTTCCTCTTCCTCTCCTTCGTCTTCGTCGTCGTTTTCTTCGGGTCAGTCGAAGAGGGTCAGGAAAGACCCGAAACCCGTGAAGCGAACCGGGTCGAAGTCGCCGGCAATGGATGCATCGCGCCAGAAAAAGTTCAGAGGCGTACGCCGGCGGCCTTGGGGTCGATGGGCTGCGGAGATCCGAGATCCGAATCGTAGAAAACGGGTCTGGCTCGGAACCTTCGACACAGCGGAGGAAGCCGCCAGTGTTTACGACAGAGCTGCCATCCTTCTCAAGGGTCCAGACGCCGTCACCAACTTTTCTAACGCCGTTACAACGGAAGAGAAAGTCGTTGCCGATGTGGTGGTCCCAGCTGCGGGGGAGGGATGCGGGTGCGTATCACCGCCGTCCAGCGAGGCTCTCTCGTCGCCCACCTCGGTCCTCCGTTACGAGGAGCAAACGCCGTTTGATGGTTTTATTTACGGAGACGTTGACGACTTCGTGTTTGATATCGACTTGCCGTTAAGCTTTCCAGATATTATGTTGTCGAGTAACAACTTTGTGCGTGACGAGTTCGGCGAGTTTGACGTAGACGATTTCTTAGCTGACGTCGTAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
AP2

Protein Analysis

306

Amino Acids

33.32

Weight (kDa)

5.3

Isoelectric Point (pI)

60.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 150 - 199 1.7e-15 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 909
AccI GTMKAC 1 cut(s) 888
AccII CGCG 1 cut(s) 439
AccIII TCCGGA 1 cut(s) 207
AclWI GGATC 4 cut(s) 157, 218, 490, 497
AcsI RAATTY 1 cut(s) 125
AcyI GRCGYC 4 cut(s) 225, 231, 603, 906
AfaI GTAC 2 cut(s) 270, 462
AfiI CCNNNNNNNGG 5 cut(s) 592, 673, 674, 748, 767
AflIII ACRYGT 1 cut(s) 286
AjnI CCWGG 1 cut(s) 165
AleI CACNNNNGTG 1 cut(s) 146
AluBI AGCT 6 cut(s) 202, 310, 578, 671, 825, 902
AluI AGCT 6 cut(s) 202, 310, 578, 671, 825, 902
Alw21I GWGCWC 1 cut(s) 312
Alw26I GTCTC 1 cut(s) 777
AlwI GGATC 4 cut(s) 157, 218, 490, 497
Ama87I CYCGRG 1 cut(s) 305
Aor13HI TCCGGA 1 cut(s) 207
AoxI GGCC 1 cut(s) 470
ApeKI GCWGC 4 cut(s) 29, 488, 578, 671
ApoI RAATTY 1 cut(s) 125
ArsI GACNNNNNNTTYG 4 cut(s) 273, 305, 785, 817
Asp700I GAANNNNTTC 2 cut(s) 89, 449
AspLEI GCGC 1 cut(s) 441
AspS9I GGNCC 3 cut(s) 596, 664, 736
AsuC2I CCSGG 1 cut(s) 409
AsuHPI GGTGA 3 cut(s) 133, 601, 690
AvaI CYCGRG 1 cut(s) 305
AvaII GGWCC 3 cut(s) 596, 664, 736
BanII GRGCYC 1 cut(s) 312
BbsI GAAGAC 2 cut(s) 264, 333
Bbv12I GWGCWC 1 cut(s) 312
BbvI GCAGC 4 cut(s) 16, 475, 565, 658
BccI CCATC 3 cut(s) 477, 590, 761
BceAI ACGGC 5 cut(s) 590, 611, 688, 745, 802
BcgI CGANNNNNNTGC 2 cut(s) 560, 594
BciT130I CCWGG 1 cut(s) 167
BcnI CCSGG 1 cut(s) 409
BcoDI GTCTC 1 cut(s) 777
BisI GCNGC 7 cut(s) 30, 265, 470, 489, 558, 579, 672
BlsI GCNGC 7 cut(s) 31, 266, 471, 490, 559, 580, 673
Bme1390I CCNGG 2 cut(s) 167, 409
Bme18I GGWCC 3 cut(s) 596, 664, 736
BmeT110I CYCGRG 1 cut(s) 305
BmgT120I GGNCC 3 cut(s) 596, 664, 736
BmiI GGNNCC 4 cut(s) 171, 535, 597, 666
BmrFI CCNGG 2 cut(s) 167, 409
BmrI ACTGGG 1 cut(s) 182
BmsI GCATC 4 cut(s) 57, 421, 443, 674
BmuI ACTGGG 1 cut(s) 182
BpiI GAAGAC 2 cut(s) 264, 333
BplI GAGNNNNNCTC 4 cut(s) 299, 331, 704, 736
BpmI CTGGAG 1 cut(s) 229
BpuEI CTTGAG 1 cut(s) 575
BpuMI CCSGG 1 cut(s) 409
BsaBI GATNNNNATC 1 cut(s) 507
BsaHI GRCGYC 4 cut(s) 225, 231, 603, 906
BsaJI CCNNGG 2 cut(s) 473, 732
BsaWI WCCGGW 1 cut(s) 207
Bsc4I CCNNNNNNNGG 5 cut(s) 592, 673, 674, 748, 767
Bse118I RCCGGY 2 cut(s) 421, 465
Bse1I ACTGG 2 cut(s) 188, 561
Bse3DI GCAATG 1 cut(s) 432
Bse8I GATNNNNATC 1 cut(s) 507
BseAI TCCGGA 1 cut(s) 207
BseBI CCWGG 1 cut(s) 167
BseDI CCNNGG 2 cut(s) 473, 732
BseGI GGATG 3 cut(s) 436, 582, 689
BseJI GATNNNNATC 1 cut(s) 507
BseLI CCNNNNNNNGG 5 cut(s) 592, 673, 674, 748, 767
BseMI GCAATG 1 cut(s) 432
BseNI ACTGG 2 cut(s) 188, 561
BseRI GAGGAG 3 cut(s) 232, 304, 764
BseXI GCAGC 4 cut(s) 16, 475, 565, 658
BseYI CCCAGC 1 cut(s) 667
Bsh1236I CGCG 1 cut(s) 439
BshFI GGCC 1 cut(s) 472
BsiHKAI GWGCWC 1 cut(s) 312
BsiHKCI CYCGRG 1 cut(s) 305
BsiSI CCGG 4 cut(s) 208, 408, 422, 466
BsiWI CGTACG 2 cut(s) 268, 460
BslFI GGGAC 2 cut(s) 176, 650
BslI CCNNNNNNNGG 5 cut(s) 592, 673, 674, 748, 767
BsmAI GTCTC 1 cut(s) 777
BsmBI CGTCTC 1 cut(s) 777
BsmFI GGGAC 2 cut(s) 176, 650
BsnI GGCC 1 cut(s) 472
BsoBI CYCGRG 1 cut(s) 305
Bsp1286I GDGCHC 1 cut(s) 312
Bsp13I TCCGGA 1 cut(s) 207
Bsp143I GATC 5 cut(s) 162, 210, 297, 495, 502
BspANI GGCC 1 cut(s) 472
BspEI TCCGGA 1 cut(s) 207
BspFNI CGCG 1 cut(s) 439
BspLI GGNNCC 4 cut(s) 171, 535, 597, 666
BspPI GGATC 4 cut(s) 157, 218, 490, 497
BsrDI GCAATG 1 cut(s) 432
BsrFI RCCGGY 2 cut(s) 421, 465
BsrI ACTGG 2 cut(s) 188, 561
BssAI RCCGGY 2 cut(s) 421, 465
BssECI CCNNGG 2 cut(s) 473, 732
BssMI GATC 5 cut(s) 162, 210, 297, 495, 502
BssNI GRCGYC 4 cut(s) 225, 231, 603, 906
BssT1I CCWWGG 1 cut(s) 473
Bst2UI CCWGG 1 cut(s) 167
Bst4CI ACNGT 1 cut(s) 148
Bst6I CTCTTC 3 cut(s) 331, 365, 633
BstACI GRCGYC 4 cut(s) 225, 231, 603, 906
BstAPI GCANNNNNTGC 2 cut(s) 116, 431
BstC8I GCNNGC 2 cut(s) 423, 467
BstDEI CTNAG 1 cut(s) 898
BstENI CCTNNNNNAGG 1 cut(s) 590
BstF5I GGATG 3 cut(s) 436, 582, 689
BstFNI CGCG 1 cut(s) 439
BstHHI GCGC 1 cut(s) 441
BstKTI GATC 5 cut(s) 165, 213, 300, 498, 505
BstMAI GTCTC 1 cut(s) 777
BstMBI GATC 5 cut(s) 162, 210, 297, 495, 502
BstMWI GCNNNNNNNGC 5 cut(s) 116, 270, 316, 431, 554
BstNI CCWGG 1 cut(s) 167
BstNSI RCATGY 1 cut(s) 290
BstSCI CCNGG 2 cut(s) 165, 407
BstUI CGCG 1 cut(s) 439
BstV1I GCAGC 4 cut(s) 16, 475, 565, 658
BstV2I GAAGAC 2 cut(s) 264, 333
BstX2I RGATCY 2 cut(s) 495, 502
BstYI RGATCY 2 cut(s) 495, 502
BsuRI GGCC 1 cut(s) 472
BtgZI GCGATG 1 cut(s) 420
BtsCI GGATG 3 cut(s) 436, 582, 689
BtsIMutI CAGTG 1 cut(s) 568
Cac8I GCNNGC 2 cut(s) 423, 467
CfoI GCGC 1 cut(s) 441
Cfr10I RCCGGY 2 cut(s) 421, 465
Cfr13I GGNCC 3 cut(s) 596, 664, 736
CseI GACGC 3 cut(s) 233, 239, 611
Csp6I GTAC 2 cut(s) 269, 461
CviAII CATG 3 cut(s) 82, 160, 287
CviQI GTAC 2 cut(s) 269, 461
DdeI CTNAG 1 cut(s) 898
DpnI GATC 5 cut(s) 164, 212, 299, 497, 504
DpnII GATC 5 cut(s) 162, 210, 297, 495, 502
Eam1104I CTCTTC 3 cut(s) 331, 365, 633
EarI CTCTTC 3 cut(s) 331, 365, 633
EciI GGCGGA 1 cut(s) 58
Ecl136II GAGCTC 1 cut(s) 310
Eco130I CCWWGG 1 cut(s) 473
Eco24I GRGCYC 1 cut(s) 312
Eco32I GATATC 1 cut(s) 808
Eco47I GGWCC 3 cut(s) 596, 664, 736
Eco53kI GAGCTC 1 cut(s) 310
Eco88I CYCGRG 1 cut(s) 305
EcoICRI GAGCTC 1 cut(s) 310
EcoNI CCTNNNNNAGG 1 cut(s) 590
EcoRII CCWGG 1 cut(s) 165
EcoRV GATATC 1 cut(s) 808
EcoT14I CCWWGG 1 cut(s) 473
EcoT22I ATGCAT 1 cut(s) 436
EcoT38I GRGCYC 1 cut(s) 312
ErhI CCWWGG 1 cut(s) 473
Esp3I CGTCTC 1 cut(s) 777
FaeI CATG 3 cut(s) 85, 163, 290
FaiI YATR 5 cut(s) 83, 161, 215, 288, 839
FaqI GGGAC 2 cut(s) 176, 650
FatI CATG 3 cut(s) 81, 159, 286
FauI CCCGC 4 cut(s) 53, 201, 667, 680
FblI GTMKAC 1 cut(s) 888
Fnu4HI GCNGC 7 cut(s) 30, 265, 470, 489, 558, 579, 672
FokI GGATG 3 cut(s) 443, 569, 696
FriOI GRGCYC 1 cut(s) 312
Fsp4HI GCNGC 7 cut(s) 30, 265, 470, 489, 558, 579, 672
GlaI GCGC 1 cut(s) 440
GluI GCNGC 7 cut(s) 30, 265, 470, 489, 558, 579, 672
GsaI CCCAGC 1 cut(s) 671
GsuI CTGGAG 1 cut(s) 229
HaeIII GGCC 1 cut(s) 472
HapII CCGG 4 cut(s) 208, 408, 422, 466
HgaI GACGC 3 cut(s) 233, 239, 611
HhaI GCGC 1 cut(s) 441
Hin1I GRCGYC 4 cut(s) 225, 231, 603, 906
Hin1II CATG 3 cut(s) 85, 163, 290
Hin6I GCGC 1 cut(s) 439
HinP1I GCGC 1 cut(s) 439
HincII GTYRAC 1 cut(s) 790
HindII GTYRAC 1 cut(s) 790
HindIII AAGCTT 1 cut(s) 823
HinfI GANTC 4 cut(s) 16, 239, 276, 508
HpaII CCGG 4 cut(s) 208, 408, 422, 466
HphI GGTGA 3 cut(s) 133, 601, 690
Hpy166II GTNNAC 3 cut(s) 568, 790, 889
Hpy188I TCNGA 7 cut(s) 37, 133, 293, 455, 500, 507, 533
Hpy188III TCNNGA 5 cut(s) 20, 208, 379, 599, 830
Hpy8I GTNNAC 3 cut(s) 568, 790, 889
Hpy99I CGWCG 8 cut(s) 16, 233, 254, 257, 349, 352, 727, 911
HpyAV CCTTC 5 cut(s) 100, 330, 345, 547, 596
HpyCH4III ACNGT 1 cut(s) 148
HpyCH4IV ACGT 3 cut(s) 786, 885, 906
HpyCH4V TGCA 1 cut(s) 434
HpyF10VI GCNNNNNNNGC 5 cut(s) 116, 270, 316, 431, 554
HpyF3I CTNAG 1 cut(s) 898
HpySE526I ACGT 3 cut(s) 786, 885, 906
Hsp92I GRCGYC 4 cut(s) 225, 231, 603, 906
Hsp92II CATG 3 cut(s) 85, 163, 290
HspAI GCGC 1 cut(s) 439
Kpn2I TCCGGA 1 cut(s) 207
KroI GCCGGC 2 cut(s) 421, 465
KroNI GCCGGC 2 cut(s) 423, 467
Kzo9I GATC 5 cut(s) 162, 210, 297, 495, 502
LmnI GCTCC 1 cut(s) 751
Lsp1109I GCAGC 4 cut(s) 16, 475, 565, 658
LweI GCATC 4 cut(s) 57, 421, 443, 674
MaeII ACGT 3 cut(s) 786, 885, 906
MaeIII GTNAC 6 cut(s) 148, 607, 628, 743, 848, 863
MalI GATC 5 cut(s) 164, 212, 299, 497, 504
MboI GATC 5 cut(s) 162, 210, 297, 495, 502
MboII GAAGA 6 cut(s) 269, 318, 333, 348, 382, 650
MflI RGATCY 2 cut(s) 495, 502
MhlI GDGCHC 1 cut(s) 312
MluCI AATT 2 cut(s) 55, 125
MlyI GAGTC 2 cut(s) 233, 285
MmeI TCCRAC 2 cut(s) 60, 118
Mph1103I ATGCAT 1 cut(s) 436
MreI CGCCGGCG 1 cut(s) 465
MroI TCCGGA 1 cut(s) 207
MroNI GCCGGC 2 cut(s) 421, 465
MroXI GAANNNNTTC 2 cut(s) 89, 449
MseI TTAA 1 cut(s) 821
MslI CAYNNNNRTG 1 cut(s) 146
MspA1I CMGCKG 3 cut(s) 264, 548, 671
MspI CCGG 4 cut(s) 208, 408, 422, 466
MspR9I CCNGG 2 cut(s) 167, 409
MvaI CCWGG 1 cut(s) 167
MvnI CGCG 1 cut(s) 439
MwoI GCNNNNNNNGC 5 cut(s) 116, 270, 316, 431, 554
NaeI GCCGGC 2 cut(s) 423, 467
NciI CCSGG 1 cut(s) 409
NdeII GATC 5 cut(s) 162, 210, 297, 495, 502
NgoMIV GCCGGC 2 cut(s) 421, 465
NlaIII CATG 3 cut(s) 85, 163, 290
NlaIV GGNNCC 4 cut(s) 171, 535, 597, 666
NmuCI GTSAC 3 cut(s) 148, 607, 863
NsiI ATGCAT 1 cut(s) 436
NspI RCATGY 1 cut(s) 290
OliI CACNNNNGTG 1 cut(s) 146
PaeR7I CTCGAG 1 cut(s) 305
PciI ACATGT 1 cut(s) 286
PcsI WCGNNNNNNNCGW 4 cut(s) 344, 365, 537, 873
PdiI GCCGGC 2 cut(s) 423, 467
PdmI GAANNNNTTC 2 cut(s) 89, 449
PfeI GAWTC 2 cut(s) 16, 508
Pfl23II CGTACG 2 cut(s) 268, 460
PflFI GACNNNGTC 1 cut(s) 605
PfoI TCCNGGA 1 cut(s) 165
PkrI GCNGC 7 cut(s) 31, 266, 471, 490, 559, 580, 673
PleI GAGTC 2 cut(s) 233, 284
PpsI GAGTC 2 cut(s) 233, 284
PscI ACATGT 1 cut(s) 286
Psp124BI GAGCTC 1 cut(s) 312
Psp6I CCWGG 1 cut(s) 165
PspFI CCCAGC 1 cut(s) 667
PspGI CCWGG 1 cut(s) 165
PspLI CGTACG 2 cut(s) 268, 460
PspN4I GGNNCC 4 cut(s) 171, 535, 597, 666
PspPI GGNCC 3 cut(s) 596, 664, 736
PspXI VCTCGAGB 1 cut(s) 305
PsuI RGATCY 2 cut(s) 495, 502
PsyI GACNNNGTC 1 cut(s) 605
PvuII CAGCTG 1 cut(s) 671
RsaI GTAC 2 cut(s) 270, 462
RsaNI GTAC 2 cut(s) 269, 461
RseI CAYNNNNRTG 1 cut(s) 146
SacI GAGCTC 1 cut(s) 312
SaqAI TTAA 1 cut(s) 821
SatI GCNGC 7 cut(s) 30, 265, 470, 489, 558, 579, 672
Sau3AI GATC 5 cut(s) 162, 210, 297, 495, 502
Sau96I GGNCC 3 cut(s) 596, 664, 736
SchI GAGTC 2 cut(s) 233, 285
ScrFI CCNGG 2 cut(s) 167, 409
SduI GDGCHC 1 cut(s) 312
SfaNI GCATC 4 cut(s) 57, 421, 443, 674
Sfr274I CTCGAG 1 cut(s) 305
SgrAI CRCCGGYG 1 cut(s) 465
SinI GGWCC 3 cut(s) 596, 664, 736
SlaI CTCGAG 1 cut(s) 305
SmiMI CAYNNNNRTG 1 cut(s) 146
SmlI CTYRAG 2 cut(s) 305, 590
SmoI CTYRAG 2 cut(s) 305, 590
Sse9I AATT 2 cut(s) 55, 125
SstI GAGCTC 1 cut(s) 312
StyD4I CCNGG 2 cut(s) 165, 407
StyI CCWWGG 1 cut(s) 473
TaaI ACNGT 1 cut(s) 148
TaiI ACGT 3 cut(s) 789, 888, 909
TaqI TCGA 8 cut(s) 14, 306, 368, 413, 481, 540, 810, 845
TaqII GACCGA 1 cut(s) 724
TasI AATT 2 cut(s) 55, 125
TauI GCSGC 3 cut(s) 267, 472, 560
TfiI GAWTC 2 cut(s) 16, 508
Tru1I TTAA 1 cut(s) 821
Tru9I TTAA 1 cut(s) 821
TscAI CASTG 1 cut(s) 568
TseFI GTSAC 3 cut(s) 148, 607, 863
TseI GCWGC 4 cut(s) 29, 488, 578, 671
Tsp45I GTSAC 3 cut(s) 148, 607, 863
TspDTI ATGAA 2 cut(s) 49, 98
TspGWI ACGGA 4 cut(s) 236, 650, 731, 795
TspRI CASTG 1 cut(s) 568
Tth111I GACNNNGTC 1 cut(s) 605
VpaK11BI GGWCC 3 cut(s) 596, 664, 736
XagI CCTNNNNNAGG 1 cut(s) 590
XapI RAATTY 1 cut(s) 125
XceI RCATGY 1 cut(s) 290
XhoI CTCGAG 1 cut(s) 305
XmiI GTMKAC 1 cut(s) 888
XmnI GAANNNNTTC 2 cut(s) 89, 449
ZraI GACGTC 1 cut(s) 907
Zsp2I ATGCAT 1 cut(s) 436
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.