MD03G1168600.v1.1

Component of the peroxisomal and mitochondrial division machineries. Plays a role in promoting the fission of mitochondria and peroxisomes

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0003617
Physical Location & Seq
Forward (+)
22800529 .. 22801853
1325 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1168600.v1.1.491

Sequence Viewer

Length: 270 bp
ATGAGGGATCCAACTAAAGACCCACTGGAAGTGAGAGAGCCACTATATCTTCTTGCTGTTGGGTATTACAGAAGTGGTGACCATACCAGGTGCAGGGAACTTGTGGAACAGAGTTTGATGATTGCACCTGATTGGAGACAGGCACTGAACCTTAAGAAAATGAACGAAGCCCGGATCAAAAGAGATGGTGCGATTGGCATAGGAATTGCAGCGACTGCTGTTGGACTGCTTGCTGGTGGAATTGCAGCGGCTGTGGCTCGGAAGAAGTGA

Protein Analysis

90

Amino Acids

9.76

Weight (kDa)

9.72

Isoelectric Point (pI)

25.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fis1_TPR_C PF14853 12 - 63 3.4e-16 Fis1 C-terminal tetratricopeptide repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 248
AclWI GGATC 3 cut(s) 2, 15, 182
AfiI CCNNNNNNNGG 1 cut(s) 93
AflII CTTAAG 1 cut(s) 152
AjnI CCWGG 1 cut(s) 86
Alw26I GTCTC 1 cut(s) 130
AlwI GGATC 3 cut(s) 2, 15, 182
AlwNI CAGNNNCTG 3 cut(s) 145, 215, 251
ApeKI GCWGC 2 cut(s) 209, 245
AsuC2I CCSGG 1 cut(s) 172
AsuHPI GGTGA 1 cut(s) 89
BamHI GGATCC 1 cut(s) 7
BbvI GCAGC 2 cut(s) 221, 257
BccI CCATC 1 cut(s) 179
BciT130I CCWGG 1 cut(s) 88
BcnI CCSGG 1 cut(s) 172
BcoDI GTCTC 1 cut(s) 130
BfrI CTTAAG 1 cut(s) 152
BisI GCNGC 3 cut(s) 210, 246, 249
BlsI GCNGC 3 cut(s) 211, 247, 250
Bme1390I CCNGG 2 cut(s) 88, 172
BmiI GGNNCC 1 cut(s) 9
BmrFI CCNGG 2 cut(s) 88, 172
BpuMI CCSGG 1 cut(s) 172
Bsc4I CCNNNNNNNGG 1 cut(s) 93
Bse1I ACTGG 1 cut(s) 30
BseBI CCWGG 1 cut(s) 88
BseLI CCNNNNNNNGG 1 cut(s) 93
BseNI ACTGG 1 cut(s) 30
BseXI GCAGC 2 cut(s) 221, 257
BsgI GTGCAG 1 cut(s) 112
BsiSI CCGG 1 cut(s) 172
BslI CCNNNNNNNGG 1 cut(s) 93
BsmAI GTCTC 1 cut(s) 130
Bsp143I GATC 2 cut(s) 7, 174
BspACI CCGC 1 cut(s) 248
BspLI GGNNCC 1 cut(s) 9
BspPI GGATC 3 cut(s) 2, 15, 182
BspTI CTTAAG 1 cut(s) 152
BsrI ACTGG 1 cut(s) 30
BssMI GATC 2 cut(s) 7, 174
Bst2UI CCWGG 1 cut(s) 88
BstAFI CTTAAG 1 cut(s) 152
BstAPI GCANNNNNTGC 1 cut(s) 215
BstC8I GCNNGC 1 cut(s) 231
BstEII GGTNACC 1 cut(s) 77
BstKTI GATC 2 cut(s) 10, 177
BstMAI GTCTC 1 cut(s) 130
BstMBI GATC 2 cut(s) 7, 174
BstMWI GCNNNNNNNGC 2 cut(s) 215, 254
BstNI CCWGG 1 cut(s) 88
BstPI GGTNACC 1 cut(s) 77
BstSCI CCNGG 2 cut(s) 86, 170
BstV1I GCAGC 2 cut(s) 221, 257
BstX2I RGATCY 1 cut(s) 7
BstYI RGATCY 1 cut(s) 7
BtsIMutI CAGTG 2 cut(s) 23, 143
Cac8I GCNNGC 1 cut(s) 231
CaiI CAGNNNCTG 3 cut(s) 145, 215, 251
CsiI ACCWGGT 1 cut(s) 86
CviJI RGCY 4 cut(s) 40, 170, 251, 257
CviKI_1 RGCY 4 cut(s) 40, 170, 251, 257
DpnI GATC 2 cut(s) 9, 176
DpnII GATC 2 cut(s) 7, 174
Eco91I GGTNACC 1 cut(s) 77
EcoO65I GGTNACC 1 cut(s) 77
EcoRII CCWGG 1 cut(s) 86
FaiI YATR 3 cut(s) 46, 84, 200
Fnu4HI GCNGC 3 cut(s) 210, 246, 249
Fsp4HI GCNGC 3 cut(s) 210, 246, 249
GluI GCNGC 3 cut(s) 210, 246, 249
HapII CCGG 1 cut(s) 172
HpaII CCGG 1 cut(s) 172
HphI GGTGA 1 cut(s) 89
Hpy188I TCNGA 1 cut(s) 261
HpyCH4V TGCA 4 cut(s) 93, 125, 209, 245
HpyF10VI GCNNNNNNNGC 2 cut(s) 215, 254
Kzo9I GATC 2 cut(s) 7, 174
LpnPI CCDG 8 cut(s) 11, 73, 79, 100, 125, 141, 185, 219
Lsp1109I GCAGC 2 cut(s) 221, 257
MabI ACCWGGT 1 cut(s) 86
MaeIII GTNAC 1 cut(s) 77
MalI GATC 2 cut(s) 9, 176
MboI GATC 2 cut(s) 7, 174
MboII GAAGA 1 cut(s) 41
MflI RGATCY 1 cut(s) 7
MluCI AATT 2 cut(s) 204, 240
MmeI TCCRAC 2 cut(s) 35, 202
MseI TTAA 1 cut(s) 153
MspA1I CMGCKG 1 cut(s) 248
MspCI CTTAAG 1 cut(s) 152
MspI CCGG 1 cut(s) 172
MspR9I CCNGG 2 cut(s) 88, 172
MvaI CCWGG 1 cut(s) 88
MwoI GCNNNNNNNGC 2 cut(s) 215, 254
NciI CCSGG 1 cut(s) 172
NdeII GATC 2 cut(s) 7, 174
NlaIV GGNNCC 1 cut(s) 9
NmuCI GTSAC 1 cut(s) 77
PkrI GCNGC 3 cut(s) 211, 247, 250
Psp6I CCWGG 1 cut(s) 86
PspEI GGTNACC 1 cut(s) 77
PspGI CCWGG 1 cut(s) 86
PspN4I GGNNCC 1 cut(s) 9
PstNI CAGNNNCTG 3 cut(s) 145, 215, 251
PsuI RGATCY 1 cut(s) 7
SaqAI TTAA 1 cut(s) 153
SatI GCNGC 3 cut(s) 210, 246, 249
Sau3AI GATC 2 cut(s) 7, 174
ScrFI CCNGG 2 cut(s) 88, 172
SetI ASST 3 cut(s) 92, 130, 153
SexAI ACCWGGT 1 cut(s) 86
SmlI CTYRAG 1 cut(s) 152
SmoI CTYRAG 1 cut(s) 152
Sse9I AATT 2 cut(s) 204, 240
SsiI CCGC 1 cut(s) 248
StyD4I CCNGG 2 cut(s) 86, 170
TasI AATT 2 cut(s) 204, 240
TauI GCSGC 1 cut(s) 251
Tru1I TTAA 1 cut(s) 153
Tru9I TTAA 1 cut(s) 153
TscAI CASTG 2 cut(s) 30, 150
TseFI GTSAC 1 cut(s) 77
TseI GCWGC 2 cut(s) 209, 245
Tsp45I GTSAC 1 cut(s) 77
TspDTI ATGAA 1 cut(s) 176
TspRI CASTG 2 cut(s) 30, 150
Vha464I CTTAAG 1 cut(s) 152
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.