Rorug05G0263500

Acts as a Mg(2 ) transporter. Can also transport other divalent cations such as Fe(2 ), Sr(2 ), Ba(2 ), Mn(2 ) and Co(2 ) but to a much less extent than Mg(2 )

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000984
Physical Location & Seq
Reverse (-)
29906758 .. 29912081
5324 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0263500.1

Sequence Viewer

Length: 990 bp
ATGGATTTCGAACCTCCATTCCAAGGAGCCTGCAAGTCCCTCTTACAAAATTCCCCAATAATTGACAAATTTCCAGAGGTTGAGGAATGTGAGCTACCTGTGATTGATCTTATCGATCTGGATTCTGATCACAGAAACAGAGACAAGTGTATGAACGAGATTGCTGAAGCTGCAAGCCAGTGGGGTTTCTTTCAAGTTGTGAATCATGGGATTTCACCAGAAGTTCTGAACAGCATACGAGATGAGCAAAAGAAGGTGTTTCATCAGCCTTTTGACAAAAAGATAGAACAAAGATTTTTGAATTTATCTCCCATTAGTTACCGTTGGGGGAATCCAAAAGCTACTTGTCTCCGGCAGTTCTCATGGTCGGAAGCCATTCATATTTCTATGGCGGACATTCCAAGCATGAATGAACAACACAAGAGTCTTAGGTCGACTATTGAATCATTTGTGAAGAATGTGTCTCAATTAGCTGAAAGCTTAGCTGAAATTTTAGCCCAGCCTTTGGGTGTCGAATCCAGTTACTTTAAAGATCACTGTCCACCAAGAACTAGTTTTCTTCGACTAAACAGATATGCTCCCTGTCCATTTTCTTCCCAAGTCTTCGGCCTCTTCGCTCACACCGATAGTGCTTTCCTAACAATAGTTCACCAAGACCAAGTTGGTGGTTTGGAATTGTTCATAGATGGAAGATGGATTGGAGTAAAACCTAATCCTGAGGCTCTGGTTGTCAACATTGGGGATTTTTTTGAGGCCCTGAGCAATGGTGTTTACAAGAGCATCAAACACAGGGTGATCACCAATCAAAAAGTTGAGAGGTTTTCTGTGGCATACTTCTACTGCCCCTCCTTCGATACCGTGATTCAGAGTCGCAGTACTACTGAACCAGCTGTGTTTAAGAGCTTCACTTTGCGGGAATATAAACAACAAACTGAAAGGGATGTTCGCGAACTAGGGGAGAAAGTAGGGCTCCCCAGATTTCTCCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mg_trans_NIPA

Protein Analysis

329

Amino Acids

37.68

Weight (kDa)

5.96

Isoelectric Point (pI)

55.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 32 - 131 4.2e-17 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 185 - 282 6.6e-30 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 505
AccI GTMKAC 1 cut(s) 434
AccII CGCG 1 cut(s) 948
AciI CCGC 2 cut(s) 392, 913
AcsI RAATTY 4 cut(s) 49, 68, 301, 489
AcuI CTGAAG 1 cut(s) 186
AdeI CACNNNGTG 1 cut(s) 793
AfaI GTAC 1 cut(s) 877
AfiI CCNNNNNNNGG 2 cut(s) 23, 505
AgsI TTSAA 3 cut(s) 194, 301, 443
AhlI ACTAGT 1 cut(s) 551
AloI GAACNNNNNNTCC 1 cut(s) 35
AluBI AGCT 8 cut(s) 94, 170, 341, 473, 480, 485, 890, 903
AluI AGCT 8 cut(s) 94, 170, 341, 473, 480, 485, 890, 903
Alw26I GTCTC 3 cut(s) 135, 353, 468
AoxI GGCC 2 cut(s) 607, 753
ApeKI GCWGC 1 cut(s) 170
ApoI RAATTY 4 cut(s) 49, 68, 301, 489
Asp700I GAANNNNTTC 1 cut(s) 375
AspS9I GGNCC 1 cut(s) 754
AsuHPI GGTGA 4 cut(s) 207, 641, 790, 805
AsuII TTCGAA 1 cut(s) 9
AxyI CCTNAGG 1 cut(s) 717
BanII GRGCYC 1 cut(s) 972
BbsI GAAGAC 1 cut(s) 595
BbvI GCAGC 1 cut(s) 157
BccI CCATC 2 cut(s) 680, 687
BclI TGATCA 2 cut(s) 127, 795
BcoDI GTCTC 3 cut(s) 135, 353, 468
BcuI ACTAGT 1 cut(s) 551
BfaI CTAG 2 cut(s) 552, 953
BisI GCNGC 1 cut(s) 171
BlpI GCTNAGC 1 cut(s) 481
BlsI GCNGC 1 cut(s) 172
BmcAI AGTACT 1 cut(s) 877
BmgT120I GGNCC 1 cut(s) 754
BmiI GGNNCC 2 cut(s) 28, 971
BmsI GCATC 1 cut(s) 789
BpiI GAAGAC 1 cut(s) 595
Bpu10I CCTNAGC 1 cut(s) 758
Bpu1102I GCTNAGC 1 cut(s) 481
Bpu14I TTCGAA 1 cut(s) 9
Bsa29I ATCGAT 1 cut(s) 114
BsaBI GATNNNNATC 1 cut(s) 126
BsaJI CCNNGG 1 cut(s) 22
BsaXI ACNNNNNCTCC 2 cut(s) 830, 860
Bsc4I CCNNNNNNNGG 2 cut(s) 23, 505
Bse1I ACTGG 2 cut(s) 178, 519
Bse21I CCTNAGG 1 cut(s) 717
Bse3DI GCAATG 1 cut(s) 769
Bse8I GATNNNNATC 1 cut(s) 126
BseCI ATCGAT 1 cut(s) 114
BseDI CCNNGG 1 cut(s) 22
BseGI GGATG 1 cut(s) 946
BseJI GATNNNNATC 1 cut(s) 126
BseLI CCNNNNNNNGG 2 cut(s) 23, 505
BseMI GCAATG 1 cut(s) 769
BseMII CTCAG 2 cut(s) 708, 749
BseNI ACTGG 2 cut(s) 178, 519
BseXI GCAGC 1 cut(s) 157
BseYI CCCAGC 1 cut(s) 498
Bsh1236I CGCG 1 cut(s) 948
BshFI GGCC 2 cut(s) 609, 755
BshVI ATCGAT 1 cut(s) 114
BsiSI CCGG 1 cut(s) 352
BslFI GGGAC 1 cut(s) 22
BslI CCNNNNNNNGG 2 cut(s) 23, 505
BsmAI GTCTC 3 cut(s) 135, 353, 468
BsmFI GGGAC 1 cut(s) 22
BsnI GGCC 2 cut(s) 609, 755
Bsp119I TTCGAA 1 cut(s) 9
Bsp1286I GDGCHC 1 cut(s) 972
Bsp143I GATC 5 cut(s) 106, 115, 127, 532, 795
Bsp1720I GCTNAGC 1 cut(s) 481
Bsp68I TCGCGA 1 cut(s) 948
BspACI CCGC 2 cut(s) 392, 913
BspANI GGCC 2 cut(s) 609, 755
BspCNI CTCAG 2 cut(s) 709, 750
BspDI ATCGAT 1 cut(s) 114
BspFNI CGCG 1 cut(s) 948
BspLI GGNNCC 2 cut(s) 28, 971
BspT104I TTCGAA 1 cut(s) 9
BsrDI GCAATG 1 cut(s) 769
BsrI ACTGG 2 cut(s) 178, 519
BssECI CCNNGG 1 cut(s) 22
BssMI GATC 5 cut(s) 106, 115, 127, 532, 795
BssT1I CCWWGG 1 cut(s) 22
Bst4CI ACNGT 3 cut(s) 323, 539, 859
Bst6I CTCTTC 1 cut(s) 617
BstBI TTCGAA 1 cut(s) 9
BstC8I GCNNGC 2 cut(s) 31, 175
BstDEI CTNAG 4 cut(s) 428, 481, 717, 758
BstF5I GGATG 1 cut(s) 946
BstFNI CGCG 1 cut(s) 948
BstKTI GATC 5 cut(s) 109, 118, 130, 535, 798
BstMAI GTCTC 3 cut(s) 135, 353, 468
BstMBI GATC 5 cut(s) 106, 115, 127, 532, 795
BstMWI GCNNNNNNNGC 1 cut(s) 170
BstUI CGCG 1 cut(s) 948
BstV1I GCAGC 1 cut(s) 157
BstV2I GAAGAC 1 cut(s) 595
BstXI CCANNNNNNTGG 1 cut(s) 665
Bsu15I ATCGAT 1 cut(s) 114
Bsu36I CCTNAGG 1 cut(s) 717
BsuRI GGCC 2 cut(s) 609, 755
BsuTUI ATCGAT 1 cut(s) 114
BtsCI GGATG 1 cut(s) 946
BtsIMutI CAGTG 2 cut(s) 185, 535
BtuMI TCGCGA 1 cut(s) 948
Cac8I GCNNGC 2 cut(s) 31, 175
Cfr13I GGNCC 1 cut(s) 754
ClaI ATCGAT 1 cut(s) 114
Csp6I GTAC 1 cut(s) 876
CviAII CATG 3 cut(s) 206, 363, 406
CviQI GTAC 1 cut(s) 876
DdeI CTNAG 4 cut(s) 428, 481, 717, 758
DpnI GATC 5 cut(s) 108, 117, 129, 534, 797
DpnII GATC 5 cut(s) 106, 115, 127, 532, 795
DraI TTTAAA 1 cut(s) 529
DraIII CACNNNGTG 1 cut(s) 793
Eam1104I CTCTTC 1 cut(s) 617
EarI CTCTTC 1 cut(s) 617
EciI GGCGGA 1 cut(s) 407
Eco130I CCWWGG 1 cut(s) 22
Eco24I GRGCYC 1 cut(s) 972
Eco57I CTGAAG 1 cut(s) 186
Eco81I CCTNAGG 1 cut(s) 717
EcoO109I RGGNCCY 1 cut(s) 754
EcoT14I CCWWGG 1 cut(s) 22
EcoT38I GRGCYC 1 cut(s) 972
ErhI CCWWGG 1 cut(s) 22
FaeI CATG 3 cut(s) 209, 366, 409
FalI AAGNNNNNCTT 2 cut(s) 26, 58
FaqI GGGAC 1 cut(s) 22
FatI CATG 3 cut(s) 205, 362, 405
FauI CCCGC 1 cut(s) 906
FbaI TGATCA 2 cut(s) 127, 795
FblI GTMKAC 1 cut(s) 434
Fnu4HI GCNGC 1 cut(s) 171
FokI GGATG 1 cut(s) 953
FriOI GRGCYC 1 cut(s) 972
Fsp4HI GCNGC 1 cut(s) 171
FspBI CTAG 2 cut(s) 552, 953
GluI GCNGC 1 cut(s) 171
GsaI CCCAGC 1 cut(s) 502
HaeIII GGCC 2 cut(s) 609, 755
HapII CCGG 1 cut(s) 352
Hin1II CATG 3 cut(s) 209, 366, 409
HincII GTYRAC 2 cut(s) 435, 733
HindII GTYRAC 2 cut(s) 435, 733
HindIII AAGCTT 1 cut(s) 478
HinfI GANTC 8 cut(s) 122, 202, 331, 424, 443, 515, 862, 868
HpaII CCGG 1 cut(s) 352
HphI GGTGA 4 cut(s) 207, 641, 790, 805
Hpy166II GTNNAC 5 cut(s) 435, 542, 649, 733, 772
Hpy188I TCNGA 4 cut(s) 127, 228, 370, 867
Hpy188III TCNNGA 4 cut(s) 74, 119, 716, 947
Hpy8I GTNNAC 5 cut(s) 435, 542, 649, 733, 772
HpyAV CCTTC 2 cut(s) 247, 859
HpyCH4III ACNGT 3 cut(s) 323, 539, 859
HpyCH4V TGCA 2 cut(s) 33, 173
HpyF10VI GCNNNNNNNGC 1 cut(s) 170
HpyF3I CTNAG 4 cut(s) 428, 481, 717, 758
Hsp92II CATG 3 cut(s) 209, 366, 409
Ksp22I TGATCA 2 cut(s) 127, 795
Kzo9I GATC 5 cut(s) 106, 115, 127, 532, 795
LmnI GCTCC 3 cut(s) 26, 583, 975
Lsp1109I GCAGC 1 cut(s) 157
LweI GCATC 1 cut(s) 789
MaeI CTAG 2 cut(s) 552, 953
MaeIII GTNAC 2 cut(s) 317, 521
MalI GATC 5 cut(s) 108, 117, 129, 534, 797
MboI GATC 5 cut(s) 106, 115, 127, 532, 795
MboII GAAGA 6 cut(s) 466, 551, 585, 595, 604, 702
MhlI GDGCHC 1 cut(s) 972
MluCI AATT 7 cut(s) 49, 60, 68, 301, 467, 489, 674
MlyI GAGTC 2 cut(s) 433, 877
MmeI TCCRAC 1 cut(s) 348
MnlI CCTC 9 cut(s) 24, 50, 70, 76, 620, 712, 745, 810, 856
MroXI GAANNNNTTC 1 cut(s) 375
MseI TTAA 3 cut(s) 528, 897, 988
MspA1I CMGCKG 1 cut(s) 890
MspI CCGG 1 cut(s) 352
MvnI CGCG 1 cut(s) 948
MwoI GCNNNNNNNGC 1 cut(s) 170
NdeII GATC 5 cut(s) 106, 115, 127, 532, 795
NlaIII CATG 3 cut(s) 209, 366, 409
NlaIV GGNNCC 2 cut(s) 28, 971
NruI TCGCGA 1 cut(s) 948
NspV TTCGAA 1 cut(s) 9
PcsI WCGNNNNNNNCGW 1 cut(s) 621
PdmI GAANNNNTTC 1 cut(s) 375
PfeI GAWTC 6 cut(s) 122, 202, 331, 443, 515, 862
PflMI CCANNNNNTGG 1 cut(s) 505
PkrI GCNGC 1 cut(s) 172
PleI GAGTC 2 cut(s) 432, 876
PpsI GAGTC 2 cut(s) 432, 876
PspFI CCCAGC 1 cut(s) 498
PspN4I GGNNCC 2 cut(s) 28, 971
PspPI GGNCC 1 cut(s) 754
PvuII CAGCTG 1 cut(s) 890
RruI TCGCGA 1 cut(s) 948
RsaI GTAC 1 cut(s) 877
RsaNI GTAC 1 cut(s) 876
SalI GTCGAC 1 cut(s) 433
SaqAI TTAA 3 cut(s) 528, 897, 988
SatI GCNGC 1 cut(s) 171
Sau3AI GATC 5 cut(s) 106, 115, 127, 532, 795
Sau96I GGNCC 1 cut(s) 754
ScaI AGTACT 1 cut(s) 877
SchI GAGTC 2 cut(s) 433, 877
SduI GDGCHC 1 cut(s) 972
SfaNI GCATC 1 cut(s) 789
SfuI TTCGAA 1 cut(s) 9
SpeI ACTAGT 1 cut(s) 551
Sse9I AATT 7 cut(s) 49, 60, 68, 301, 467, 489, 674
SsiI CCGC 2 cut(s) 392, 913
SspMI CTAG 2 cut(s) 552, 953
StyI CCWWGG 1 cut(s) 22
TaaI ACNGT 3 cut(s) 323, 539, 859
TaqI TCGA 6 cut(s) 9, 114, 434, 513, 562, 852
TasI AATT 7 cut(s) 49, 60, 68, 301, 467, 489, 674
TatI WGTACW 1 cut(s) 875
TfiI GAWTC 6 cut(s) 122, 202, 331, 443, 515, 862
Tru1I TTAA 3 cut(s) 528, 897, 988
Tru9I TTAA 3 cut(s) 528, 897, 988
TscAI CASTG 2 cut(s) 185, 542
TseI GCWGC 1 cut(s) 170
TspDTI ATGAA 6 cut(s) 167, 251, 368, 422, 426, 670
TspRI CASTG 2 cut(s) 185, 542
Van91I CCANNNNNTGG 1 cut(s) 505
XapI RAATTY 4 cut(s) 49, 68, 301, 489
XcmI CCANNNNNNNNNTGG 1 cut(s) 659
XmiI GTMKAC 1 cut(s) 434
XmnI GAANNNNTTC 1 cut(s) 375
XspI CTAG 2 cut(s) 552, 953
ZrmI AGTACT 1 cut(s) 877
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.