FvH4_1g25650

Acts as a Mg(2 ) transporter. Can also transport other divalent cations such as Fe(2 ), Sr(2 ), Ba(2 ), Mn(2 ) and Co(2 ) but to a much less extent than Mg(2 )

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0000984
Physical Location & Seq
Forward (+)
17501103 .. 17506214
5112 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g25650.t2

Sequence Viewer

Length: 1035 bp
ATGTCCTCGAGCAATTTGAAAGGGTTCATATTGGCCGTGGTCTCTAGCGCCTTCATTGGCTCCAGCTTCATCATCAAGAAGAAGGGCCTCCGCCGCGCCGGCAACAACGGAACCCGTGCCAGTTCTGGAGGATATGGGTACCTGCTGGAGCCTCTATGGTGGATAGGAATGGTCACAATGGTTGTTGGAGAGATATCAAATTTTGTAGCATACATTTACGCACCCGCCGGGCTTGTGACGCCGCTCGGTGCATTGAGTATCATTGTTAGTGCCGTGTTAGCGCATTTTATTTTAAAGGAGAAACTCCAGAAAATGGGAATGTTGGGCTGCCTTTTATGCATAGTGGGTTCGGTTGTGATTGTACTCCATGCACCCGAAGAGCAGACTATCACTTCCGTACAAGAAATCTGGGATTTAGCTATACAACCAGCTTTCCTTTTGTATACAGCCTCAGTGATAGCTGTAACGCTAGTCCTCATCTTGTATTGTTCTCCCCGTTATGGTCAAACCAATATACTGATTTATATAGGCATATGCTCAATTATTGGATCATTAACTGTCATGAGCATAAAAGCAATTGGTATTGCAATAAAACTCACAATAGAGGGCACAAACCAGGCAATATACTTCCAAACATGGATTTTTGCAATGGTTGCAGTTACATGTATCATCACTCAATTAAATTATCTAAACATGGCATTGGACACTTTCAACACAGCAATTGTTTCTCCCATCTATTATGCTATGTTCACATCTTTCACAATACTTGCAAGCGGAATAATGTTTAAGGATTATTCTGGTCAAAGCGCAAGCAGCATAGCATCAGAACTTTGTGGATTCATCACTGTGTTATCTGGTACTGCTATATTGCACAGTACAAGAGAACCAGATCCACCTTTAATTACAGATTTGTATACACCATTATCTCCTAAAGTATCGTGGTACATCCAAAGCAACGGAGAATGGAAGCAGAAGGATGAAGAGGGTTCAAACCCTAATTTCATCACAGTACTTCGGCAAGACTATTTCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mg_trans_NIPA

Protein Analysis

345

Amino Acids

37.46

Weight (kDa)

7.55

Isoelectric Point (pI)

32.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mg_trans_NIPA PF05653 5 - 295 3.9e-131 Magnesium transporter NIPA
EamA PF00892 55 - 121 2.8e-07 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 150
Acc65I GGTACC 1 cut(s) 138
AccB1I GGYRCC 1 cut(s) 138
AccB7I CCANNNNNTGG 1 cut(s) 313
AccBSI CCGCTC 1 cut(s) 244
AccI GTMKAC 2 cut(s) 443, 914
AccII CGCG 1 cut(s) 96
AciI CCGC 5 cut(s) 91, 94, 225, 242, 774
AclWI GGATC 2 cut(s) 556, 884
AcoI YGGCCR 1 cut(s) 33
AcsI RAATTY 1 cut(s) 199
AcyI GRCGYC 1 cut(s) 239
AfaI GTAC 7 cut(s) 140, 363, 399, 859, 877, 944, 1011
AfiI CCNNNNNNNGG 2 cut(s) 313, 500
AflIII ACRYGT 1 cut(s) 662
AgsI TTSAA 4 cut(s) 19, 712, 990, 1030
AjnI CCWGG 1 cut(s) 615
AluBI AGCT 4 cut(s) 66, 419, 431, 461
AluI AGCT 4 cut(s) 66, 419, 431, 461
Alw26I GTCTC 1 cut(s) 46
AlwI GGATC 2 cut(s) 556, 884
Ama87I CYCGRG 1 cut(s) 7
AoxI GGCC 2 cut(s) 33, 85
ApeKI GCWGC 2 cut(s) 327, 813
ApoI RAATTY 1 cut(s) 199
Asp700I GAANNNNTTC 1 cut(s) 23
Asp718I GGTACC 1 cut(s) 138
AspLEI GCGC 4 cut(s) 50, 98, 283, 809
AspS9I GGNCC 1 cut(s) 85
AsuC2I CCSGG 1 cut(s) 229
AvaI CYCGRG 1 cut(s) 7
BaeGI GKGCMC 1 cut(s) 611
BanI GGYRCC 1 cut(s) 138
BbvI GCAGC 2 cut(s) 314, 825
BccI CCATC 1 cut(s) 740
BceAI ACGGC 2 cut(s) 20, 257
BciT130I CCWGG 1 cut(s) 617
BcnI CCSGG 1 cut(s) 229
BcoDI GTCTC 1 cut(s) 46
BfaI CTAG 2 cut(s) 45, 470
BfoI RGCGCY 1 cut(s) 51
BfuAI ACCTGC 1 cut(s) 150
BglI GCCNNNNNGGC 1 cut(s) 99
BisI GCNGC 4 cut(s) 94, 242, 328, 814
BlsI GCNGC 4 cut(s) 95, 243, 329, 815
BmcAI AGTACT 1 cut(s) 1011
Bme1390I CCNGG 2 cut(s) 229, 617
BmeT110I CYCGRG 1 cut(s) 7
BmgT120I GGNCC 1 cut(s) 85
BmiI GGNNCC 4 cut(s) 61, 112, 140, 150
BmrFI CCNGG 2 cut(s) 229, 617
BmsI GCATC 1 cut(s) 830
BpmI CTGGAG 4 cut(s) 46, 147, 167, 290
BpuMI CCSGG 1 cut(s) 229
BsaHI GRCGYC 1 cut(s) 239
BsaI GGTCTC 1 cut(s) 46
BsaJI CCNNGG 1 cut(s) 36
Bsc4I CCNNNNNNNGG 2 cut(s) 313, 500
Bse118I RCCGGY 1 cut(s) 98
Bse1I ACTGG 1 cut(s) 120
Bse3DI GCAATG 1 cut(s) 654
BseBI CCWGG 1 cut(s) 617
BseDI CCNNGG 1 cut(s) 36
BseGI GGATG 2 cut(s) 945, 982
BseLI CCNNNNNNNGG 2 cut(s) 313, 500
BseMI GCAATG 1 cut(s) 654
BseMII CTCAG 1 cut(s) 465
BseNI ACTGG 1 cut(s) 120
BseSI GKGCMC 1 cut(s) 611
BseXI GCAGC 2 cut(s) 314, 825
Bsh1236I CGCG 1 cut(s) 96
BshFI GGCC 2 cut(s) 35, 87
BshNI GGYRCC 1 cut(s) 138
BsiHKCI CYCGRG 1 cut(s) 7
BsiSI CCGG 2 cut(s) 99, 228
BslI CCNNNNNNNGG 2 cut(s) 313, 500
BsmAI GTCTC 1 cut(s) 46
BsnI GGCC 2 cut(s) 35, 87
Bso31I GGTCTC 1 cut(s) 46
BsoBI CYCGRG 1 cut(s) 7
Bsp1286I GDGCHC 1 cut(s) 611
Bsp143I GATC 2 cut(s) 548, 889
BspACI CCGC 5 cut(s) 91, 94, 225, 242, 774
BspANI GGCC 2 cut(s) 35, 87
BspCNI CTCAG 1 cut(s) 464
BspFNI CGCG 1 cut(s) 96
BspHI TCATGA 1 cut(s) 561
BspLI GGNNCC 4 cut(s) 61, 112, 140, 150
BspMI ACCTGC 1 cut(s) 150
BspPI GGATC 2 cut(s) 556, 884
BspQI GCTCTTC 1 cut(s) 372
BspT107I GGYRCC 1 cut(s) 138
BspTNI GGTCTC 1 cut(s) 46
BsrBI CCGCTC 1 cut(s) 244
BsrDI GCAATG 1 cut(s) 654
BsrFI RCCGGY 1 cut(s) 98
BsrI ACTGG 1 cut(s) 120
BssAI RCCGGY 1 cut(s) 98
BssECI CCNNGG 1 cut(s) 36
BssMI GATC 2 cut(s) 548, 889
BssNAI GTATAC 2 cut(s) 444, 915
BssNI GRCGYC 1 cut(s) 239
Bst1107I GTATAC 2 cut(s) 444, 915
Bst2UI CCWGG 1 cut(s) 617
Bst4CI ACNGT 4 cut(s) 559, 847, 875, 1009
Bst6I CTCTTC 2 cut(s) 372, 975
BstACI GRCGYC 1 cut(s) 239
BstAPI GCANNNNNTGC 1 cut(s) 653
BstC8I GCNNGC 3 cut(s) 100, 772, 811
BstDEI CTNAG 1 cut(s) 451
BstDSI CCRYGG 1 cut(s) 36
BstF5I GGATG 2 cut(s) 945, 982
BstFNI CGCG 1 cut(s) 96
BstH2I RGCGCY 1 cut(s) 51
BstHHI GCGC 4 cut(s) 50, 98, 283, 809
BstKTI GATC 2 cut(s) 551, 892
BstMAI GTCTC 1 cut(s) 46
BstMBI GATC 2 cut(s) 548, 889
BstMWI GCNNNNNNNGC 7 cut(s) 93, 99, 238, 278, 336, 653, 813
BstNI CCWGG 1 cut(s) 617
BstNSI RCATGY 1 cut(s) 666
BstSCI CCNGG 2 cut(s) 227, 615
BstSLI GKGCMC 1 cut(s) 611
BstUI CGCG 1 cut(s) 96
BstV1I GCAGC 2 cut(s) 314, 825
BstX2I RGATCY 1 cut(s) 889
BstYI RGATCY 1 cut(s) 889
BstZ17I GTATAC 2 cut(s) 444, 915
BsuRI GGCC 2 cut(s) 35, 87
BtgI CCRYGG 1 cut(s) 36
BtsCI GGATG 2 cut(s) 945, 982
BtsIMutI CAGTG 2 cut(s) 459, 843
BveI ACCTGC 1 cut(s) 150
Cac8I GCNNGC 3 cut(s) 100, 772, 811
CciI TCATGA 1 cut(s) 561
CfoI GCGC 4 cut(s) 50, 98, 283, 809
Cfr10I RCCGGY 1 cut(s) 98
Cfr13I GGNCC 1 cut(s) 85
CseI GACGC 1 cut(s) 247
Csp6I GTAC 7 cut(s) 139, 362, 398, 858, 876, 943, 1010
CviAII CATG 5 cut(s) 368, 562, 636, 663, 694
CviQI GTAC 7 cut(s) 139, 362, 398, 858, 876, 943, 1010
DdeI CTNAG 1 cut(s) 451
DpnI GATC 2 cut(s) 550, 891
DpnII GATC 2 cut(s) 548, 889
DraI TTTAAA 1 cut(s) 294
EaeI YGGCCR 1 cut(s) 33
Eam1104I CTCTTC 2 cut(s) 372, 975
EarI CTCTTC 2 cut(s) 372, 975
EciI GGCGGA 1 cut(s) 80
Eco31I GGTCTC 1 cut(s) 46
Eco32I GATATC 1 cut(s) 195
Eco88I CYCGRG 1 cut(s) 7
EcoO109I RGGNCCY 1 cut(s) 85
EcoRII CCWGG 1 cut(s) 615
EcoRV GATATC 1 cut(s) 195
EcoT22I ATGCAT 1 cut(s) 341
FaeI CATG 5 cut(s) 371, 565, 639, 666, 697
FatI CATG 5 cut(s) 367, 561, 635, 662, 693
FauI CCCGC 1 cut(s) 232
FauNDI CATATG 1 cut(s) 533
FblI GTMKAC 2 cut(s) 443, 914
Fnu4HI GCNGC 4 cut(s) 94, 242, 328, 814
FokI GGATG 2 cut(s) 932, 989
Fsp4HI GCNGC 4 cut(s) 94, 242, 328, 814
FspBI CTAG 2 cut(s) 45, 470
GlaI GCGC 4 cut(s) 49, 97, 282, 808
GluI GCNGC 4 cut(s) 94, 242, 328, 814
GsuI CTGGAG 4 cut(s) 46, 147, 167, 290
HaeII RGCGCY 1 cut(s) 51
HaeIII GGCC 2 cut(s) 35, 87
HapII CCGG 2 cut(s) 99, 228
HgaI GACGC 1 cut(s) 247
HhaI GCGC 4 cut(s) 50, 98, 283, 809
Hin1I GRCGYC 1 cut(s) 239
Hin1II CATG 5 cut(s) 371, 565, 639, 666, 697
Hin6I GCGC 4 cut(s) 48, 96, 281, 807
HinP1I GCGC 4 cut(s) 48, 96, 281, 807
HinfI GANTC 1 cut(s) 837
HpaII CCGG 2 cut(s) 99, 228
Hpy166II GTNNAC 3 cut(s) 444, 750, 915
Hpy188I TCNGA 1 cut(s) 826
Hpy188III TCNNGA 4 cut(s) 76, 126, 307, 562
Hpy8I GTNNAC 3 cut(s) 444, 750, 915
HpyAV CCTTC 3 cut(s) 61, 76, 967
HpyCH4III ACNGT 4 cut(s) 559, 847, 875, 1009
HpyCH4V TGCA 8 cut(s) 251, 339, 371, 587, 647, 656, 770, 871
HpyF10VI GCNNNNNNNGC 7 cut(s) 93, 99, 238, 278, 336, 653, 813
HpyF3I CTNAG 1 cut(s) 451
Hsp92I GRCGYC 1 cut(s) 239
Hsp92II CATG 5 cut(s) 371, 565, 639, 666, 697
HspAI GCGC 4 cut(s) 48, 96, 281, 807
KpnI GGTACC 1 cut(s) 142
KroI GCCGGC 1 cut(s) 98
KroNI GCCGGC 1 cut(s) 100
Kzo9I GATC 2 cut(s) 548, 889
LguI GCTCTTC 1 cut(s) 372
LmnI GCTCC 2 cut(s) 65, 148
Lsp1109I GCAGC 2 cut(s) 314, 825
LweI GCATC 1 cut(s) 830
MaeI CTAG 2 cut(s) 45, 470
MaeIII GTNAC 4 cut(s) 172, 235, 463, 658
MalI GATC 2 cut(s) 550, 891
MbiI CCGCTC 1 cut(s) 244
MboI GATC 2 cut(s) 548, 889
MboII GAAGA 3 cut(s) 91, 389, 992
MfeI CAATTG 2 cut(s) 576, 720
MflI RGATCY 1 cut(s) 889
MhlI GDGCHC 1 cut(s) 611
MluCI AATT 9 cut(s) 13, 199, 540, 576, 677, 682, 720, 900, 997
MmeI TCCRAC 1 cut(s) 166
MnlI CCTC 8 cut(s) 16, 98, 122, 162, 460, 485, 598, 976
Mph1103I ATGCAT 1 cut(s) 341
MroNI GCCGGC 1 cut(s) 98
MroXI GAANNNNTTC 1 cut(s) 23
MseI TTAA 5 cut(s) 293, 554, 680, 786, 899
MslI CAYNNNNRTG 1 cut(s) 845
MspI CCGG 2 cut(s) 99, 228
MspR9I CCNGG 2 cut(s) 229, 617
MunI CAATTG 2 cut(s) 576, 720
MvaI CCWGG 1 cut(s) 617
MvnI CGCG 1 cut(s) 96
MwoI GCNNNNNNNGC 7 cut(s) 93, 99, 238, 278, 336, 653, 813
NaeI GCCGGC 1 cut(s) 100
NciI CCSGG 1 cut(s) 229
NdeI CATATG 1 cut(s) 533
NdeII GATC 2 cut(s) 548, 889
NgoMIV GCCGGC 1 cut(s) 98
NlaIII CATG 5 cut(s) 371, 565, 639, 666, 697
NlaIV GGNNCC 4 cut(s) 61, 112, 140, 150
NmuCI GTSAC 2 cut(s) 172, 235
NsiI ATGCAT 1 cut(s) 341
NspI RCATGY 1 cut(s) 666
PaeR7I CTCGAG 1 cut(s) 7
PagI TCATGA 1 cut(s) 561
PciI ACATGT 1 cut(s) 662
PciSI GCTCTTC 1 cut(s) 372
PdiI GCCGGC 1 cut(s) 100
PdmI GAANNNNTTC 1 cut(s) 23
PfeI GAWTC 1 cut(s) 837
PflMI CCANNNNNTGG 1 cut(s) 313
PkrI GCNGC 4 cut(s) 95, 243, 329, 815
PscI ACATGT 1 cut(s) 662
Psp6I CCWGG 1 cut(s) 615
PspGI CCWGG 1 cut(s) 615
PspN4I GGNNCC 4 cut(s) 61, 112, 140, 150
PspPI GGNCC 1 cut(s) 85
PspXI VCTCGAGB 1 cut(s) 7
PsuI RGATCY 1 cut(s) 889
RsaI GTAC 7 cut(s) 140, 363, 399, 859, 877, 944, 1011
RsaNI GTAC 7 cut(s) 139, 362, 398, 858, 876, 943, 1010
RseI CAYNNNNRTG 1 cut(s) 845
SapI GCTCTTC 1 cut(s) 372
SaqAI TTAA 5 cut(s) 293, 554, 680, 786, 899
SatI GCNGC 4 cut(s) 94, 242, 328, 814
Sau3AI GATC 2 cut(s) 548, 889
Sau96I GGNCC 1 cut(s) 85
ScaI AGTACT 1 cut(s) 1011
ScrFI CCNGG 2 cut(s) 229, 617
SduI GDGCHC 1 cut(s) 611
SetI ASST 6 cut(s) 68, 144, 421, 433, 463, 898
SfaNI GCATC 1 cut(s) 830
Sfr274I CTCGAG 1 cut(s) 7
SlaI CTCGAG 1 cut(s) 7
SmiMI CAYNNNNRTG 1 cut(s) 845
SmlI CTYRAG 1 cut(s) 7
SmoI CTYRAG 1 cut(s) 7
Sse9I AATT 9 cut(s) 13, 199, 540, 576, 677, 682, 720, 900, 997
SsiI CCGC 5 cut(s) 91, 94, 225, 242, 774
SspMI CTAG 2 cut(s) 45, 470
StyD4I CCNGG 2 cut(s) 227, 615
TaaI ACNGT 4 cut(s) 559, 847, 875, 1009
TaqI TCGA 1 cut(s) 8
TasI AATT 9 cut(s) 13, 199, 540, 576, 677, 682, 720, 900, 997
TatI WGTACW 3 cut(s) 361, 875, 1009
TauI GCSGC 2 cut(s) 96, 244
TfiI GAWTC 1 cut(s) 837
Tru1I TTAA 5 cut(s) 293, 554, 680, 786, 899
Tru9I TTAA 5 cut(s) 293, 554, 680, 786, 899
TscAI CASTG 2 cut(s) 459, 850
TseFI GTSAC 2 cut(s) 172, 235
TseI GCWGC 2 cut(s) 327, 813
Tsp45I GTSAC 2 cut(s) 172, 235
TspDTI ATGAA 6 cut(s) 16, 43, 58, 829, 991, 993
TspGWI ACGGA 3 cut(s) 123, 385, 972
TspRI CASTG 2 cut(s) 459, 850
Van91I CCANNNNNTGG 1 cut(s) 313
XapI RAATTY 1 cut(s) 199
XceI RCATGY 1 cut(s) 666
XhoI CTCGAG 1 cut(s) 7
XmiI GTMKAC 2 cut(s) 443, 914
XmnI GAANNNNTTC 1 cut(s) 23
XspI CTAG 2 cut(s) 45, 470
ZrmI AGTACT 1 cut(s) 1011
Zsp2I ATGCAT 1 cut(s) 341
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.