MD03G1168400.v1.1

Acts as a Mg(2 ) transporter. Can also transport other divalent cations such as Fe(2 ), Sr(2 ), Ba(2 ), Mn(2 ) and Co(2 ) but to a much less extent than Mg(2 )

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0000984
Physical Location & Seq
Forward (+)
22790124 .. 22794122
3999 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1168400.v1.1.491

Sequence Viewer

Length: 1050 bp
ATGGGTTTCTCCAAAGACAATCTTAAAGGCGTTCTTCTGGCTTTGCTTTCAAGTGGCTTCATCGGGGCAAGCTTTATCATCAAGAAGAAAGGCCTCAGAAAAGCCGCAGCTGCTTCTGGAGTCAGAGCTGGGTATGGCGGGTATTCTTACCTCTTGGAGCCTCTTTGGTGGGTGGGATTTATTACCATGATCGTTGGAGAGATTGCAAACTTCGTTGCATATGCATTTGCTCCAGCTGTTCTTGTTACCCCTCTCGGTGCGTTAAGTATAATTGTCAGTGCTGTGCTTGCGCATTTTATCTTGAAGGAGAGGCTGCACCGCCTTGGAGTTTTGGGCTGCGTAATGTGCATTTCCGGGTCAGTCATAATTGTTATCCATGCACCACAGGAACATTCTATCACTTCAGTTCTTGAAATATGGAGCATGGCAACTCAACCAGCTTTCTTGCTTTATGTGGGTTCAGTGATTGTTCTGGTTTTCATACTGGTCTTCCATTTTGTGCCACAATGTGGGCACACGAATGTGCTAGTTTTCACGGGCATTTGTTCATTGATGGGTTCTCTCTCGGTGATGAGTGTTAAAGCCCTAGGAACTGCTCTGAAGTTAACTTTTGAGGGGAAGAATCAGTTAATCTATCCAGAGACGTGGTTTTTTATGTTGGTTGTAGCTACATGTGTCATCACCCAAATGAATTATCTGAATAAGGCACTTGACACATTCAACACTGCGGTTGTGTCCCCTATTTATTACGTCATGTTTACCACGCTGGCAATCATTGCCAGTGTCATAATGTTTAAGGATTGGGATGACCAAAGTGGGGCAGCCATCATATCAGAAATATGTGGATTTATTGTTGTATTATGTGGAACCATCGTATTGCATTCGACGAGAGAGTTTGATAGAAGCTCGTCTTTTCGAGGTAGTTATATGCCTGTGTCGCCTTCATTATCTGCGCGACTTTGTAATGGGAATGGAGAATTATTGAAGCATGAAGAAGAAAACGGCTCACCTTCTGAGGAAATTTGCTTAAGAAGACCAGAATTGTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mg_trans_NIPA

Protein Analysis

350

Amino Acids

37.96

Weight (kDa)

7.58

Isoelectric Point (pI)

31.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mg_trans_NIPA PF05653 6 - 298 3.3e-131 Magnesium transporter NIPA
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 291
AccB7I CCANNNNNTGG 1 cut(s) 509
AccII CGCG 1 cut(s) 955
AciI CCGC 4 cut(s) 105, 138, 319, 728
AcsI RAATTY 1 cut(s) 1020
AcuI CTGAAG 2 cut(s) 387, 620
AdeI CACNNNGTG 1 cut(s) 509
AfaI GTAC 1 cut(s) 1046
AfiI CCNNNNNNNGG 2 cut(s) 509, 817
AflII CTTAAG 1 cut(s) 1027
AflIII ACRYGT 1 cut(s) 671
AgsI TTSAA 5 cut(s) 51, 304, 413, 721, 985
AjiI CACGTC 1 cut(s) 645
AleI CACNNNNGTG 1 cut(s) 521
AluBI AGCT 7 cut(s) 72, 110, 128, 236, 440, 668, 906
AluI AGCT 7 cut(s) 72, 110, 128, 236, 440, 668, 906
Alw26I GTCTC 1 cut(s) 635
AoxI GGCC 1 cut(s) 91
ApeKI GCWGC 5 cut(s) 107, 110, 313, 336, 821
ApoI RAATTY 1 cut(s) 1020
AspA2I CCTAGG 1 cut(s) 586
AspLEI GCGC 2 cut(s) 292, 955
AsuC2I CCSGG 1 cut(s) 355
AsuHPI GGTGA 3 cut(s) 580, 673, 999
AvrII CCTAGG 1 cut(s) 586
BaeGI GKGCMC 1 cut(s) 516
BbsI GAAGAC 2 cut(s) 481, 1039
BbvI GCAGC 5 cut(s) 97, 119, 300, 323, 833
BccI CCATC 3 cut(s) 547, 833, 878
BceAI ACGGC 1 cut(s) 1018
BcnI CCSGG 1 cut(s) 355
BcoDI GTCTC 1 cut(s) 635
BfaI CTAG 3 cut(s) 527, 587, 1048
BfrI CTTAAG 1 cut(s) 1027
BisI GCNGC 6 cut(s) 105, 108, 111, 314, 337, 822
BlnI CCTAGG 1 cut(s) 586
BlsI GCNGC 6 cut(s) 106, 109, 112, 315, 338, 823
Bme1390I CCNGG 1 cut(s) 355
BmgBI CACGTC 1 cut(s) 645
BmiI GGNNCC 2 cut(s) 159, 868
BmrFI CCNGG 1 cut(s) 355
BpiI GAAGAC 2 cut(s) 481, 1039
BpmI CTGGAG 2 cut(s) 138, 216
BpuMI CCSGG 1 cut(s) 355
BsaJI CCNNGG 2 cut(s) 322, 586
Bsc4I CCNNNNNNNGG 2 cut(s) 509, 817
Bse1I ACTGG 2 cut(s) 489, 780
Bse3DI GCAATG 1 cut(s) 774
BseDI CCNNGG 2 cut(s) 322, 586
BseGI GGATG 1 cut(s) 811
BseLI CCNNNNNNNGG 2 cut(s) 509, 817
BseMI GCAATG 1 cut(s) 774
BseMII CTCAG 2 cut(s) 109, 1005
BseNI ACTGG 2 cut(s) 489, 780
BseSI GKGCMC 1 cut(s) 516
BseXI GCAGC 5 cut(s) 97, 119, 300, 323, 833
BseYI CCCAGC 1 cut(s) 128
BsgI GTGCAG 1 cut(s) 299
Bsh1236I CGCG 1 cut(s) 955
BshFI GGCC 1 cut(s) 93
BsiSI CCGG 1 cut(s) 354
BslFI GGGAC 1 cut(s) 721
BslI CCNNNNNNNGG 2 cut(s) 509, 817
BsmAI GTCTC 1 cut(s) 635
BsmBI CGTCTC 1 cut(s) 635
BsmFI GGGAC 1 cut(s) 721
BsmI GAATGC 1 cut(s) 880
BsnI GGCC 1 cut(s) 93
Bsp1286I GDGCHC 1 cut(s) 516
Bsp143I GATC 1 cut(s) 189
BspACI CCGC 4 cut(s) 105, 138, 319, 728
BspANI GGCC 1 cut(s) 93
BspCNI CTCAG 2 cut(s) 108, 1006
BspFNI CGCG 1 cut(s) 955
BspLI GGNNCC 2 cut(s) 159, 868
BspTI CTTAAG 1 cut(s) 1027
BsrDI GCAATG 1 cut(s) 774
BsrI ACTGG 2 cut(s) 489, 780
BssECI CCNNGG 2 cut(s) 322, 586
BssMI GATC 1 cut(s) 189
BssT1I CCWWGG 2 cut(s) 322, 586
BstAFI CTTAAG 1 cut(s) 1027
BstAPI GCANNNNNTGC 1 cut(s) 776
BstC8I GCNNGC 3 cut(s) 70, 288, 768
BstDEI CTNAG 2 cut(s) 95, 1014
BstF5I GGATG 1 cut(s) 811
BstFNI CGCG 1 cut(s) 955
BstHHI GCGC 2 cut(s) 292, 955
BstKTI GATC 1 cut(s) 192
BstMAI GTCTC 1 cut(s) 635
BstMBI GATC 1 cut(s) 189
BstMWI GCNNNNNNNGC 5 cut(s) 110, 287, 345, 776, 937
BstNSI RCATGY 1 cut(s) 675
BstSCI CCNGG 1 cut(s) 353
BstSLI GKGCMC 1 cut(s) 516
BstUI CGCG 1 cut(s) 955
BstV1I GCAGC 5 cut(s) 97, 119, 300, 323, 833
BstV2I GAAGAC 2 cut(s) 481, 1039
BstXI CCANNNNNNTGG 1 cut(s) 645
BsuRI GGCC 1 cut(s) 93
BtrI CACGTC 1 cut(s) 645
BtsCI GGATG 1 cut(s) 811
BtsI GCAGTG 1 cut(s) 723
BtsIMutI CAGTG 4 cut(s) 283, 468, 723, 787
Cac8I GCNNGC 3 cut(s) 70, 288, 768
CfoI GCGC 2 cut(s) 292, 955
Csp6I GTAC 1 cut(s) 1045
CviAII CATG 6 cut(s) 187, 377, 424, 672, 754, 989
CviQI GTAC 1 cut(s) 1045
DdeI CTNAG 2 cut(s) 95, 1014
DpnI GATC 1 cut(s) 191
DpnII GATC 1 cut(s) 189
DraIII CACNNNGTG 1 cut(s) 509
Eco130I CCWWGG 2 cut(s) 322, 586
Eco147I AGGCCT 1 cut(s) 93
Eco57I CTGAAG 2 cut(s) 387, 620
EcoT14I CCWWGG 2 cut(s) 322, 586
EcoT22I ATGCAT 1 cut(s) 226
ErhI CCWWGG 2 cut(s) 322, 586
Esp3I CGTCTC 1 cut(s) 635
FaeI CATG 6 cut(s) 190, 380, 427, 675, 757, 992
FalI AAGNNNNNCTT 5 cut(s) 38, 18, 50, 895, 927
FaqI GGGAC 1 cut(s) 721
FatI CATG 6 cut(s) 186, 376, 423, 671, 753, 988
FauI CCCGC 1 cut(s) 131
FauNDI CATATG 1 cut(s) 220
Fnu4HI GCNGC 6 cut(s) 105, 108, 111, 314, 337, 822
FokI GGATG 1 cut(s) 818
Fsp4HI GCNGC 6 cut(s) 105, 108, 111, 314, 337, 822
FspBI CTAG 3 cut(s) 527, 587, 1048
FspI TGCGCA 1 cut(s) 291
GlaI GCGC 2 cut(s) 291, 954
GluI GCNGC 6 cut(s) 105, 108, 111, 314, 337, 822
GsaI CCCAGC 1 cut(s) 132
GsuI CTGGAG 2 cut(s) 138, 216
HaeIII GGCC 1 cut(s) 93
HapII CCGG 1 cut(s) 354
HhaI GCGC 2 cut(s) 292, 955
Hin1II CATG 6 cut(s) 190, 380, 427, 675, 757, 992
Hin6I GCGC 2 cut(s) 290, 953
HinP1I GCGC 2 cut(s) 290, 953
HincII GTYRAC 1 cut(s) 606
HindII GTYRAC 1 cut(s) 606
HindIII AAGCTT 1 cut(s) 70
HinfI GANTC 2 cut(s) 120, 622
HpaI GTTAAC 1 cut(s) 606
HpaII CCGG 1 cut(s) 354
HphI GGTGA 3 cut(s) 580, 673, 999
Hpy166II GTNNAC 2 cut(s) 606, 759
Hpy188I TCNGA 6 cut(s) 98, 125, 600, 699, 835, 1015
Hpy188III TCNNGA 5 cut(s) 82, 117, 301, 410, 638
Hpy8I GTNNAC 2 cut(s) 606, 759
Hpy99I CGWCG 1 cut(s) 889
HpyAV CCTTC 3 cut(s) 298, 951, 1020
HpyCH4IV ACGT 2 cut(s) 644, 750
HpyCH4V TGCA 7 cut(s) 206, 218, 224, 316, 348, 380, 880
HpyF10VI GCNNNNNNNGC 5 cut(s) 110, 287, 345, 776, 937
HpyF3I CTNAG 2 cut(s) 95, 1014
HpySE526I ACGT 2 cut(s) 644, 750
Hsp92II CATG 6 cut(s) 190, 380, 427, 675, 757, 992
HspAI GCGC 2 cut(s) 290, 953
KspAI GTTAAC 1 cut(s) 606
Kzo9I GATC 1 cut(s) 189
LmnI GCTCC 3 cut(s) 157, 235, 420
Lsp1109I GCAGC 5 cut(s) 97, 119, 300, 323, 833
MaeI CTAG 3 cut(s) 527, 587, 1048
MaeII ACGT 2 cut(s) 644, 750
MaeIII GTNAC 1 cut(s) 244
MalI GATC 1 cut(s) 191
MboI GATC 1 cut(s) 189
MboII GAAGA 7 cut(s) 26, 97, 481, 631, 1004, 1007, 1044
MhlI GDGCHC 1 cut(s) 516
MluCI AATT 6 cut(s) 270, 366, 691, 977, 1020, 1040
MlyI GAGTC 1 cut(s) 129
MmeI TCCRAC 1 cut(s) 175
MnlI CCTC 8 cut(s) 104, 161, 171, 261, 303, 607, 911, 1009
Mph1103I ATGCAT 1 cut(s) 226
MseI TTAA 7 cut(s) 24, 263, 579, 605, 629, 795, 1028
MslI CAYNNNNRTG 3 cut(s) 519, 521, 686
MspA1I CMGCKG 2 cut(s) 110, 236
MspCI CTTAAG 1 cut(s) 1027
MspI CCGG 1 cut(s) 354
MspR9I CCNGG 1 cut(s) 355
Mva1269I GAATGC 1 cut(s) 880
MvnI CGCG 1 cut(s) 955
MwoI GCNNNNNNNGC 5 cut(s) 110, 287, 345, 776, 937
NciI CCSGG 1 cut(s) 355
NdeI CATATG 1 cut(s) 220
NdeII GATC 1 cut(s) 189
NlaIII CATG 6 cut(s) 190, 380, 427, 675, 757, 992
NlaIV GGNNCC 2 cut(s) 159, 868
NsbI TGCGCA 1 cut(s) 291
NsiI ATGCAT 1 cut(s) 226
NspI RCATGY 1 cut(s) 675
OliI CACNNNNGTG 1 cut(s) 521
PceI AGGCCT 1 cut(s) 93
PciI ACATGT 1 cut(s) 671
PctI GAATGC 1 cut(s) 880
PfeI GAWTC 1 cut(s) 622
PflMI CCANNNNNTGG 1 cut(s) 509
PkrI GCNGC 6 cut(s) 106, 109, 112, 315, 338, 823
PleI GAGTC 1 cut(s) 128
PpsI GAGTC 1 cut(s) 128
PscI ACATGT 1 cut(s) 671
PspFI CCCAGC 1 cut(s) 128
PspN4I GGNNCC 2 cut(s) 159, 868
PvuII CAGCTG 2 cut(s) 110, 236
RsaI GTAC 1 cut(s) 1046
RsaNI GTAC 1 cut(s) 1045
RseI CAYNNNNRTG 3 cut(s) 519, 521, 686
SaqAI TTAA 7 cut(s) 24, 263, 579, 605, 629, 795, 1028
SatI GCNGC 6 cut(s) 105, 108, 111, 314, 337, 822
Sau3AI GATC 1 cut(s) 189
SchI GAGTC 1 cut(s) 129
ScrFI CCNGG 1 cut(s) 355
SduI GDGCHC 1 cut(s) 516
SmiMI CAYNNNNRTG 3 cut(s) 519, 521, 686
SmlI CTYRAG 1 cut(s) 1027
SmoI CTYRAG 1 cut(s) 1027
Sse9I AATT 6 cut(s) 270, 366, 691, 977, 1020, 1040
SseBI AGGCCT 1 cut(s) 93
SsiI CCGC 4 cut(s) 105, 138, 319, 728
SspMI CTAG 3 cut(s) 527, 587, 1048
StuI AGGCCT 1 cut(s) 93
StyD4I CCNGG 1 cut(s) 353
StyI CCWWGG 2 cut(s) 322, 586
TaiI ACGT 2 cut(s) 647, 753
TaqI TCGA 2 cut(s) 884, 916
TasI AATT 6 cut(s) 270, 366, 691, 977, 1020, 1040
TatI WGTACW 1 cut(s) 1044
TauI GCSGC 1 cut(s) 107
TfiI GAWTC 1 cut(s) 622
Tru1I TTAA 7 cut(s) 24, 263, 579, 605, 629, 795, 1028
Tru9I TTAA 7 cut(s) 24, 263, 579, 605, 629, 795, 1028
TscAI CASTG 4 cut(s) 283, 468, 730, 787
TseI GCWGC 5 cut(s) 107, 110, 313, 336, 821
TspDTI ATGAA 6 cut(s) 49, 469, 537, 704, 933, 1005
TspRI CASTG 4 cut(s) 283, 468, 730, 787
Van91I CCANNNNNTGG 1 cut(s) 509
Vha464I CTTAAG 1 cut(s) 1027
XapI RAATTY 1 cut(s) 1020
XceI RCATGY 1 cut(s) 675
XmaJI CCTAGG 1 cut(s) 586
XspI CTAG 3 cut(s) 527, 587, 1048
Zsp2I ATGCAT 1 cut(s) 226
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.