Rh2AG307700

Acts as a Mg(2 ) transporter. Can also transport other divalent cations such as Fe(2 ), Sr(2 ), Ba(2 ), Mn(2 ) and Co(2 ) but to a much less extent than Mg(2 )

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0000984
Physical Location & Seq
Forward (+)
39078607 .. 39081722
3116 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG307700.1

Sequence Viewer

Length: 636 bp
ATGTCATCGAGCAATTTGAAAGGGTTCATATTGGCCGTGGTCTCAAGCGCCTTCATTGGCTCCAGCTTCATCATCAAGAAGAAGGGTCTCCGCCGAGCCGGCAACAATGGAACTCGTGCCAGTTCTGGAGGATATGGGTATCTGCGGGAGCCTCTATGGTGGATAGGAATGGTCACAATGGTTGTTGGAGAGATAGCAAATTTTGTAGCATACATATACGCACCTGCCGGGCTTGTCACGCCGCTTGGTGCTTTGAGTATCATTGTTAGTGCGGTGTTAGCGCATTTCCTGTTAAAGGAGAAACTCCAGAAAATGGGAATGTTGGGCTGCCTTTTATGCATAGTGGGTTCGGTTGTGATTGTACTCCATGCACCCGAAGAGCAGACTCTTACTTCCGTCCAACAAATCTGGGATTTAGCAATACAACCAGCTTTCCTTTTGTATACAGCCTCAGTGATAGCTGTAACGCTAGTCCTTATCTTGTATTGTTCCCCCCGCTATGGCCAAACCAATATACTGATTTATATAGGAATCTGCTCAATTATTGGATCATTGACTATAACTTTCTCTTCTTTATCCTCTGCACTTTTTTGTTATGTATTCTTTTGTTTTAGAAATCTAAGAGATGGGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mg_trans_NIPA

Protein Analysis

211

Amino Acids

22.84

Weight (kDa)

9.22

Isoelectric Point (pI)

36.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mg_trans_NIPA PF05653 5 - 188 2.3e-83 Magnesium transporter NIPA
EamA PF00892 55 - 121 8.5e-08 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 232
Acc36I ACCTGC 1 cut(s) 232
AccB7I CCANNNNNTGG 1 cut(s) 313
AccI GTMKAC 1 cut(s) 443
AciI CCGC 5 cut(s) 91, 145, 242, 272, 496
AclWI GGATC 1 cut(s) 556
AcoI YGGCCR 2 cut(s) 33, 502
AcsI RAATTY 1 cut(s) 199
AfaI GTAC 1 cut(s) 363
AfiI CCNNNNNNNGG 3 cut(s) 295, 313, 500
AgsI TTSAA 1 cut(s) 19
AluBI AGCT 3 cut(s) 66, 431, 461
AluI AGCT 3 cut(s) 66, 431, 461
Alw26I GTCTC 2 cut(s) 46, 92
AlwI GGATC 1 cut(s) 556
AoxI GGCC 2 cut(s) 33, 502
ApeKI GCWGC 1 cut(s) 327
ApoI RAATTY 1 cut(s) 199
Asp700I GAANNNNTTC 1 cut(s) 23
AspLEI GCGC 2 cut(s) 50, 283
AsuC2I CCSGG 1 cut(s) 229
BalI TGGCCA 1 cut(s) 504
BauI CACGAG 1 cut(s) 114
BbvI GCAGC 1 cut(s) 314
BccI CCATC 1 cut(s) 620
BceAI ACGGC 1 cut(s) 20
BcnI CCSGG 1 cut(s) 229
BcoDI GTCTC 2 cut(s) 46, 92
BfaI CTAG 1 cut(s) 470
BfoI RGCGCY 1 cut(s) 51
BfuAI ACCTGC 1 cut(s) 232
BglI GCCNNNNNGGC 1 cut(s) 99
BisI GCNGC 2 cut(s) 242, 328
BlsI GCNGC 2 cut(s) 243, 329
Bme1390I CCNGG 1 cut(s) 229
BmiI GGNNCC 2 cut(s) 61, 150
BmrFI CCNGG 1 cut(s) 229
BpmI CTGGAG 3 cut(s) 46, 147, 290
BpuEI CTTGAG 1 cut(s) 28
BpuMI CCSGG 1 cut(s) 229
BsaI GGTCTC 2 cut(s) 46, 92
BsaJI CCNNGG 1 cut(s) 36
Bsc4I CCNNNNNNNGG 3 cut(s) 295, 313, 500
Bse118I RCCGGY 1 cut(s) 98
Bse1I ACTGG 1 cut(s) 120
BseDI CCNNGG 1 cut(s) 36
BseLI CCNNNNNNNGG 3 cut(s) 295, 313, 500
BseMII CTCAG 1 cut(s) 465
BseNI ACTGG 1 cut(s) 120
BseXI GCAGC 1 cut(s) 314
BsgI GTGCAG 1 cut(s) 567
BshFI GGCC 2 cut(s) 35, 504
BsiSI CCGG 2 cut(s) 99, 228
BslI CCNNNNNNNGG 3 cut(s) 295, 313, 500
BsmAI GTCTC 2 cut(s) 46, 92
BsnI GGCC 2 cut(s) 35, 504
Bso31I GGTCTC 2 cut(s) 46, 92
Bsp143I GATC 1 cut(s) 548
BspACI CCGC 5 cut(s) 91, 145, 242, 272, 496
BspANI GGCC 2 cut(s) 35, 504
BspCNI CTCAG 1 cut(s) 464
BspLI GGNNCC 2 cut(s) 61, 150
BspMI ACCTGC 1 cut(s) 232
BspPI GGATC 1 cut(s) 556
BspQI GCTCTTC 1 cut(s) 372
BspTNI GGTCTC 2 cut(s) 46, 92
BsrFI RCCGGY 1 cut(s) 98
BsrI ACTGG 1 cut(s) 120
BssAI RCCGGY 1 cut(s) 98
BssECI CCNNGG 1 cut(s) 36
BssMI GATC 1 cut(s) 548
BssNAI GTATAC 1 cut(s) 444
BssSI CACGAG 1 cut(s) 114
Bst1107I GTATAC 1 cut(s) 444
Bst2BI CACGAG 1 cut(s) 114
Bst6I CTCTTC 2 cut(s) 372, 574
BstC8I GCNNGC 1 cut(s) 100
BstDEI CTNAG 2 cut(s) 451, 620
BstDSI CCRYGG 1 cut(s) 36
BstENI CCTNNNNNAGG 1 cut(s) 293
BstH2I RGCGCY 1 cut(s) 51
BstHHI GCGC 2 cut(s) 50, 283
BstKTI GATC 1 cut(s) 551
BstMAI GTCTC 2 cut(s) 46, 92
BstMBI GATC 1 cut(s) 548
BstMWI GCNNNNNNNGC 4 cut(s) 99, 238, 278, 336
BstSCI CCNGG 1 cut(s) 227
BstV1I GCAGC 1 cut(s) 314
BstZ17I GTATAC 1 cut(s) 444
BsuRI GGCC 2 cut(s) 35, 504
BtgI CCRYGG 1 cut(s) 36
BtsIMutI CAGTG 1 cut(s) 459
BveI ACCTGC 1 cut(s) 232
Cac8I GCNNGC 1 cut(s) 100
CfoI GCGC 2 cut(s) 50, 283
Cfr10I RCCGGY 1 cut(s) 98
Csp6I GTAC 1 cut(s) 362
CviAII CATG 1 cut(s) 368
CviQI GTAC 1 cut(s) 362
DdeI CTNAG 2 cut(s) 451, 620
DpnI GATC 1 cut(s) 550
DpnII GATC 1 cut(s) 548
EaeI YGGCCR 2 cut(s) 33, 502
Eam1104I CTCTTC 2 cut(s) 372, 574
EarI CTCTTC 2 cut(s) 372, 574
EciI GGCGGA 1 cut(s) 80
Eco31I GGTCTC 2 cut(s) 46, 92
EcoNI CCTNNNNNAGG 1 cut(s) 293
EcoT22I ATGCAT 1 cut(s) 341
FaeI CATG 1 cut(s) 371
FatI CATG 1 cut(s) 367
FauI CCCGC 2 cut(s) 138, 503
FblI GTMKAC 1 cut(s) 443
Fnu4HI GCNGC 2 cut(s) 242, 328
Fsp4HI GCNGC 2 cut(s) 242, 328
FspBI CTAG 1 cut(s) 470
GlaI GCGC 2 cut(s) 49, 282
GluI GCNGC 2 cut(s) 242, 328
GsuI CTGGAG 3 cut(s) 46, 147, 290
HaeII RGCGCY 1 cut(s) 51
HaeIII GGCC 2 cut(s) 35, 504
HapII CCGG 2 cut(s) 99, 228
HhaI GCGC 2 cut(s) 50, 283
Hin1II CATG 1 cut(s) 371
Hin6I GCGC 2 cut(s) 48, 281
HinP1I GCGC 2 cut(s) 48, 281
HinfI GANTC 2 cut(s) 385, 531
HpaII CCGG 2 cut(s) 99, 228
Hpy166II GTNNAC 1 cut(s) 444
Hpy188III TCNNGA 3 cut(s) 76, 126, 307
Hpy8I GTNNAC 1 cut(s) 444
HpyAV CCTTC 2 cut(s) 61, 76
HpyCH4V TGCA 3 cut(s) 339, 371, 584
HpyF10VI GCNNNNNNNGC 4 cut(s) 99, 238, 278, 336
HpyF3I CTNAG 2 cut(s) 451, 620
Hsp92II CATG 1 cut(s) 371
HspAI GCGC 2 cut(s) 48, 281
KroI GCCGGC 1 cut(s) 98
KroNI GCCGGC 1 cut(s) 100
Kzo9I GATC 1 cut(s) 548
LguI GCTCTTC 1 cut(s) 372
LmnI GCTCC 2 cut(s) 65, 148
Lsp1109I GCAGC 1 cut(s) 314
MaeI CTAG 1 cut(s) 470
MaeIII GTNAC 3 cut(s) 172, 235, 463
MalI GATC 1 cut(s) 550
MboI GATC 1 cut(s) 548
MboII GAAGA 3 cut(s) 91, 389, 561
MlsI TGGCCA 1 cut(s) 504
MluCI AATT 3 cut(s) 13, 199, 540
MluNI TGGCCA 1 cut(s) 504
MlyI GAGTC 1 cut(s) 379
MmeI TCCRAC 2 cut(s) 166, 424
MnlI CCTC 4 cut(s) 122, 162, 460, 589
Mox20I TGGCCA 1 cut(s) 504
Mph1103I ATGCAT 1 cut(s) 341
MroNI GCCGGC 1 cut(s) 98
MroXI GAANNNNTTC 1 cut(s) 23
MscI TGGCCA 1 cut(s) 504
MseI TTAA 1 cut(s) 293
Msp20I TGGCCA 1 cut(s) 504
MspI CCGG 2 cut(s) 99, 228
MspR9I CCNGG 1 cut(s) 229
MwoI GCNNNNNNNGC 4 cut(s) 99, 238, 278, 336
NaeI GCCGGC 1 cut(s) 100
NciI CCSGG 1 cut(s) 229
NdeII GATC 1 cut(s) 548
NgoMIV GCCGGC 1 cut(s) 98
NlaIII CATG 1 cut(s) 371
NlaIV GGNNCC 2 cut(s) 61, 150
NmeAIII GCCGAG 1 cut(s) 119
NmuCI GTSAC 2 cut(s) 172, 235
NsiI ATGCAT 1 cut(s) 341
PaqCI CACCTGC 1 cut(s) 232
PciSI GCTCTTC 1 cut(s) 372
PdiI GCCGGC 1 cut(s) 100
PdmI GAANNNNTTC 1 cut(s) 23
PfeI GAWTC 1 cut(s) 531
PflMI CCANNNNNTGG 1 cut(s) 313
PkrI GCNGC 2 cut(s) 243, 329
PleI GAGTC 1 cut(s) 379
PpsI GAGTC 1 cut(s) 379
PspN4I GGNNCC 2 cut(s) 61, 150
RsaI GTAC 1 cut(s) 363
RsaNI GTAC 1 cut(s) 362
SapI GCTCTTC 1 cut(s) 372
SaqAI TTAA 1 cut(s) 293
SatI GCNGC 2 cut(s) 242, 328
Sau3AI GATC 1 cut(s) 548
SchI GAGTC 1 cut(s) 379
ScrFI CCNGG 1 cut(s) 229
SetI ASST 4 cut(s) 68, 226, 433, 463
SmlI CTYRAG 1 cut(s) 43
SmoI CTYRAG 1 cut(s) 43
Sse9I AATT 3 cut(s) 13, 199, 540
SsiI CCGC 5 cut(s) 91, 145, 242, 272, 496
SspMI CTAG 1 cut(s) 470
StyD4I CCNGG 1 cut(s) 227
TaqI TCGA 1 cut(s) 8
TasI AATT 3 cut(s) 13, 199, 540
TatI WGTACW 1 cut(s) 361
TauI GCSGC 1 cut(s) 244
TfiI GAWTC 1 cut(s) 531
Tru1I TTAA 1 cut(s) 293
Tru9I TTAA 1 cut(s) 293
TscAI CASTG 1 cut(s) 459
TseFI GTSAC 2 cut(s) 172, 235
TseI GCWGC 1 cut(s) 327
Tsp45I GTSAC 2 cut(s) 172, 235
TspDTI ATGAA 3 cut(s) 16, 43, 58
TspGWI ACGGA 1 cut(s) 385
TspRI CASTG 1 cut(s) 459
Van91I CCANNNNNTGG 1 cut(s) 313
XagI CCTNNNNNAGG 1 cut(s) 293
XapI RAATTY 1 cut(s) 199
XmiI GTMKAC 1 cut(s) 443
XmnI GAANNNNTTC 1 cut(s) 23
XspI CTAG 1 cut(s) 470
Zsp2I ATGCAT 1 cut(s) 341
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.