pycom520g00770

Acts as a Mg(2 ) transporter. Can also transport other divalent cations such as Fe(2 ), Sr(2 ), Ba(2 ), Mn(2 ) and Co(2 ) but to a much less extent than Mg(2 )

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_520
OG0000984
Physical Location & Seq
Forward (+)
625411 .. 629570
4160 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom520g00770.9

Sequence Viewer

Length: 912 bp
ATGTACTCAAGCAATTTGAAAGGCTTCATCTTGGCCGTCGTCTCGAGCGCCTTCATTGGCTCCAGCTTCATCATCAAGAAGAAGGGTCTCCGCCGGGCCAGTGTCAACGGCCCTCGAGCCAGTGTGGGAGGATACGGGTATCTGTTAGAACCTCTATGGTGGATAGGAATGGTAACAATGGTTGTTGGAGAGATAGCCAATTTTGTAGCATACATTTACGCACCTGCTGGCCTGGTCACCCCGCTTGGTGCATTGAGCATTATTGTTAGTGCTGTTTTAGCGCATTTTTTGTTGAAGGAGAAACTCCAGAAAATGGGAATGTTGGGGTGTCTTTTATGCATAGTGGGTTCGACTGTGATTGTACTCCATTCACCTGAAGAGCAATCTCTCAGTTCCGTCGAAGAAATCTGGGAATTAGCAATACAACCTGCATTTCTTTTGTATACAGCCTCGGTGGTAGCTGTAACATTAGTTCTGATGTTGTTTTGTGCTCCCCGCTATGGCCAAACCAATATACTGATTTATACAGGAATCTGTTCTATAATAGGATCGTTGACAGTTATGAGTGTAAAAGCAATTGGGATTGCTATAGAACTCACAATAGAGGGCACAAACCAGCTAAAATACTTCCAAACATGGATTTTTGGAATGGTTGCAGTTACCTGTATCATCACTCAATTGAATTATCTGAACATGGCGTTGGATACTTTCAACACAGCAATTGTTTCTCCGATATATTATGCAATGTTCACATCTTTTACAATATTTGCCAGTGCAATAATGTTTAAGGATTATTCGGGTCAAAGTGTGAGCAGCATAGCATCAGAATTTTGCGGTTTCATCACTGTGTTATCTGGGACCGCTATATTGCATAGTACAAGAGAGCAAGATCCACCTTTAATTACAGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mg_trans_NIPA

Protein Analysis

304

Amino Acids

32.79

Weight (kDa)

6.3

Isoelectric Point (pI)

33.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 232
Acc36I ACCTGC 2 cut(s) 232, 436
AccB7I CCANNNNNTGG 1 cut(s) 313
AccI GTMKAC 1 cut(s) 443
AciI CCGC 5 cut(s) 91, 242, 496, 834, 861
AclWI GGATC 2 cut(s) 556, 884
AcoI YGGCCR 2 cut(s) 33, 502
AcsI RAATTY 1 cut(s) 827
AcuI CTGAAG 1 cut(s) 396
AfaI GTAC 3 cut(s) 5, 363, 877
AfiI CCNNNNNNNGG 2 cut(s) 313, 500
AgsI TTSAA 4 cut(s) 19, 295, 682, 712
AjnI CCWGG 1 cut(s) 231
AjuI GAANNNNNNNTTGG 2 cut(s) 683, 715
AluBI AGCT 3 cut(s) 66, 461, 619
AluI AGCT 3 cut(s) 66, 461, 619
Alw21I GWGCWC 1 cut(s) 493
Alw26I GTCTC 2 cut(s) 46, 92
AlwI GGATC 2 cut(s) 556, 884
Ama87I CYCGRG 2 cut(s) 43, 114
AoxI GGCC 5 cut(s) 33, 96, 109, 229, 502
ApeKI GCWGC 1 cut(s) 813
ApoI RAATTY 1 cut(s) 827
Asp700I GAANNNNTTC 2 cut(s) 23, 535
AspLEI GCGC 2 cut(s) 50, 283
AspS9I GGNCC 3 cut(s) 96, 110, 858
AsuC2I CCSGG 1 cut(s) 95
AsuHPI GGTGA 2 cut(s) 229, 363
AvaI CYCGRG 2 cut(s) 43, 114
AvaII GGWCC 1 cut(s) 858
BaeGI GKGCMC 1 cut(s) 611
BalI TGGCCA 1 cut(s) 504
Bbv12I GWGCWC 1 cut(s) 493
BbvI GCAGC 1 cut(s) 825
BceAI ACGGC 2 cut(s) 20, 124
BciT130I CCWGG 1 cut(s) 233
BciVI GTATCC 2 cut(s) 125, 697
BcnI CCSGG 1 cut(s) 95
BcoDI GTCTC 2 cut(s) 46, 92
BfmI CTRYAG 1 cut(s) 588
BfoI RGCGCY 1 cut(s) 51
BfuAI ACCTGC 2 cut(s) 232, 436
BfuI GTATCC 2 cut(s) 125, 697
BisI GCNGC 1 cut(s) 814
BlsI GCNGC 1 cut(s) 815
Bme1390I CCNGG 2 cut(s) 95, 233
Bme18I GGWCC 1 cut(s) 858
BmeT110I CYCGRG 2 cut(s) 43, 114
BmgT120I GGNCC 3 cut(s) 96, 110, 858
BmiI GGNNCC 2 cut(s) 61, 859
BmrFI CCNGG 2 cut(s) 95, 233
BmsI GCATC 1 cut(s) 830
BpmI CTGGAG 2 cut(s) 46, 290
BpuMI CCSGG 1 cut(s) 95
BsaI GGTCTC 1 cut(s) 92
BsaJI CCNNGG 1 cut(s) 450
Bsc4I CCNNNNNNNGG 2 cut(s) 313, 500
Bse1I ACTGG 3 cut(s) 99, 120, 771
Bse3DI GCAATG 1 cut(s) 750
BseBI CCWGG 1 cut(s) 233
BseDI CCNNGG 1 cut(s) 450
BseLI CCNNNNNNNGG 2 cut(s) 313, 500
BseMI GCAATG 1 cut(s) 750
BseMII CTCAG 1 cut(s) 403
BseNI ACTGG 3 cut(s) 99, 120, 771
BseSI GKGCMC 1 cut(s) 611
BseXI GCAGC 1 cut(s) 825
BshFI GGCC 5 cut(s) 35, 98, 111, 231, 504
BsiHKAI GWGCWC 1 cut(s) 493
BsiHKCI CYCGRG 2 cut(s) 43, 114
BsiSI CCGG 1 cut(s) 94
BslFI GGGAC 1 cut(s) 871
BslI CCNNNNNNNGG 2 cut(s) 313, 500
BsmAI GTCTC 2 cut(s) 46, 92
BsmBI CGTCTC 1 cut(s) 46
BsmFI GGGAC 1 cut(s) 871
BsnI GGCC 5 cut(s) 35, 98, 111, 231, 504
Bso31I GGTCTC 1 cut(s) 92
BsoBI CYCGRG 2 cut(s) 43, 114
Bsp1286I GDGCHC 2 cut(s) 493, 611
Bsp143I GATC 2 cut(s) 548, 889
BspACI CCGC 5 cut(s) 91, 242, 496, 834, 861
BspANI GGCC 5 cut(s) 35, 98, 111, 231, 504
BspCNI CTCAG 1 cut(s) 402
BspLI GGNNCC 2 cut(s) 61, 859
BspMI ACCTGC 2 cut(s) 232, 436
BspPI GGATC 2 cut(s) 556, 884
BspQI GCTCTTC 1 cut(s) 372
BspTNI GGTCTC 1 cut(s) 92
BsrDI GCAATG 1 cut(s) 750
BsrI ACTGG 3 cut(s) 99, 120, 771
BssECI CCNNGG 1 cut(s) 450
BssMI GATC 2 cut(s) 548, 889
BssNAI GTATAC 1 cut(s) 444
Bst1107I GTATAC 1 cut(s) 444
Bst2UI CCWGG 1 cut(s) 233
Bst4CI ACNGT 3 cut(s) 355, 559, 847
Bst6I CTCTTC 1 cut(s) 372
BstC8I GCNNGC 1 cut(s) 229
BstDEI CTNAG 1 cut(s) 389
BstEII GGTNACC 1 cut(s) 235
BstH2I RGCGCY 1 cut(s) 51
BstHHI GCGC 2 cut(s) 50, 283
BstKTI GATC 2 cut(s) 551, 892
BstMAI GTCTC 2 cut(s) 46, 92
BstMBI GATC 2 cut(s) 548, 889
BstMWI GCNNNNNNNGC 1 cut(s) 278
BstNI CCWGG 1 cut(s) 233
BstPI GGTNACC 1 cut(s) 235
BstSCI CCNGG 2 cut(s) 93, 231
BstSFI CTRYAG 1 cut(s) 588
BstSLI GKGCMC 1 cut(s) 611
BstV1I GCAGC 1 cut(s) 825
BstX2I RGATCY 1 cut(s) 889
BstYI RGATCY 1 cut(s) 889
BstZ17I GTATAC 1 cut(s) 444
BsuI GTATCC 2 cut(s) 125, 697
BsuRI GGCC 5 cut(s) 35, 98, 111, 231, 504
BtsIMutI CAGTG 4 cut(s) 106, 127, 778, 843
BveI ACCTGC 2 cut(s) 232, 436
Cac8I GCNNGC 1 cut(s) 229
CfoI GCGC 2 cut(s) 50, 283
Cfr13I GGNCC 3 cut(s) 96, 110, 858
Csp6I GTAC 3 cut(s) 4, 362, 876
CviAII CATG 2 cut(s) 636, 694
CviQI GTAC 3 cut(s) 4, 362, 876
DdeI CTNAG 1 cut(s) 389
DpnI GATC 2 cut(s) 550, 891
DpnII GATC 2 cut(s) 548, 889
EaeI YGGCCR 2 cut(s) 33, 502
Eam1104I CTCTTC 1 cut(s) 372
EarI CTCTTC 1 cut(s) 372
EciI GGCGGA 1 cut(s) 80
Eco31I GGTCTC 1 cut(s) 92
Eco47I GGWCC 1 cut(s) 858
Eco57I CTGAAG 1 cut(s) 396
Eco88I CYCGRG 2 cut(s) 43, 114
Eco91I GGTNACC 1 cut(s) 235
EcoO65I GGTNACC 1 cut(s) 235
EcoRII CCWGG 1 cut(s) 231
EcoT22I ATGCAT 1 cut(s) 341
Esp3I CGTCTC 1 cut(s) 46
FaeI CATG 2 cut(s) 639, 697
FaqI GGGAC 1 cut(s) 871
FatI CATG 2 cut(s) 635, 693
FauI CCCGC 2 cut(s) 249, 503
FblI GTMKAC 1 cut(s) 443
Fnu4HI GCNGC 1 cut(s) 814
Fsp4HI GCNGC 1 cut(s) 814
GlaI GCGC 2 cut(s) 49, 282
GluI GCNGC 1 cut(s) 814
GsuI CTGGAG 2 cut(s) 46, 290
HaeII RGCGCY 1 cut(s) 51
HaeIII GGCC 5 cut(s) 35, 98, 111, 231, 504
HapII CCGG 1 cut(s) 94
HhaI GCGC 2 cut(s) 50, 283
Hin1II CATG 2 cut(s) 639, 697
Hin6I GCGC 2 cut(s) 48, 281
HinP1I GCGC 2 cut(s) 48, 281
HincII GTYRAC 2 cut(s) 106, 555
HindII GTYRAC 2 cut(s) 106, 555
HinfI GANTC 1 cut(s) 531
HpaII CCGG 1 cut(s) 94
HphI GGTGA 2 cut(s) 229, 363
Hpy166II GTNNAC 4 cut(s) 106, 444, 555, 750
Hpy188I TCNGA 4 cut(s) 477, 690, 732, 826
Hpy188III TCNNGA 3 cut(s) 43, 76, 307
Hpy8I GTNNAC 4 cut(s) 106, 444, 555, 750
Hpy99I CGWCG 2 cut(s) 41, 401
HpyAV CCTTC 3 cut(s) 61, 76, 289
HpyCH4III ACNGT 3 cut(s) 355, 559, 847
HpyCH4V TGCA 7 cut(s) 251, 339, 431, 656, 743, 776, 871
HpyF10VI GCNNNNNNNGC 1 cut(s) 278
HpyF3I CTNAG 1 cut(s) 389
Hsp92II CATG 2 cut(s) 639, 697
HspAI GCGC 2 cut(s) 48, 281
Kzo9I GATC 2 cut(s) 548, 889
LguI GCTCTTC 1 cut(s) 372
LmnI GCTCC 2 cut(s) 65, 496
Lsp1109I GCAGC 1 cut(s) 825
LweI GCATC 1 cut(s) 830
MaeIII GTNAC 4 cut(s) 172, 235, 463, 658
MalI GATC 2 cut(s) 550, 891
MboI GATC 2 cut(s) 548, 889
MboII GAAGA 3 cut(s) 91, 389, 413
MfeI CAATTG 3 cut(s) 576, 677, 720
MflI RGATCY 1 cut(s) 889
MhlI GDGCHC 2 cut(s) 493, 611
MlsI TGGCCA 1 cut(s) 504
MluCI AATT 9 cut(s) 13, 199, 413, 576, 677, 682, 720, 827, 900
MluNI TGGCCA 1 cut(s) 504
MmeI TCCRAC 2 cut(s) 166, 681
MnlI CCTC 5 cut(s) 122, 123, 162, 460, 598
Mox20I TGGCCA 1 cut(s) 504
Mph1103I ATGCAT 1 cut(s) 341
MroXI GAANNNNTTC 2 cut(s) 23, 535
MscI TGGCCA 1 cut(s) 504
MseI TTAA 2 cut(s) 786, 899
MslI CAYNNNNRTG 1 cut(s) 845
Msp20I TGGCCA 1 cut(s) 504
MspI CCGG 1 cut(s) 94
MspR9I CCNGG 2 cut(s) 95, 233
MunI CAATTG 3 cut(s) 576, 677, 720
MvaI CCWGG 1 cut(s) 233
MwoI GCNNNNNNNGC 1 cut(s) 278
NciI CCSGG 1 cut(s) 95
NdeII GATC 2 cut(s) 548, 889
NlaIII CATG 2 cut(s) 639, 697
NlaIV GGNNCC 2 cut(s) 61, 859
NmuCI GTSAC 1 cut(s) 235
NsiI ATGCAT 1 cut(s) 341
PaeR7I CTCGAG 2 cut(s) 43, 114
PaqCI CACCTGC 1 cut(s) 232
PciSI GCTCTTC 1 cut(s) 372
PdmI GAANNNNTTC 2 cut(s) 23, 535
PfeI GAWTC 1 cut(s) 531
PflMI CCANNNNNTGG 1 cut(s) 313
PkrI GCNGC 1 cut(s) 815
Psp6I CCWGG 1 cut(s) 231
PspEI GGTNACC 1 cut(s) 235
PspGI CCWGG 1 cut(s) 231
PspN4I GGNNCC 2 cut(s) 61, 859
PspPI GGNCC 3 cut(s) 96, 110, 858
PspXI VCTCGAGB 1 cut(s) 114
PsrI GAACNNNNNNTAC 2 cut(s) 456, 488
PsuI RGATCY 1 cut(s) 889
RsaI GTAC 3 cut(s) 5, 363, 877
RsaNI GTAC 3 cut(s) 4, 362, 876
RseI CAYNNNNRTG 1 cut(s) 845
SapI GCTCTTC 1 cut(s) 372
SaqAI TTAA 2 cut(s) 786, 899
SatI GCNGC 1 cut(s) 814
Sau3AI GATC 2 cut(s) 548, 889
Sau96I GGNCC 3 cut(s) 96, 110, 858
ScrFI CCNGG 2 cut(s) 95, 233
SduI GDGCHC 2 cut(s) 493, 611
SetI ASST 9 cut(s) 68, 154, 226, 376, 430, 463, 621, 665, 898
SfaNI GCATC 1 cut(s) 830
SfcI CTRYAG 1 cut(s) 588
Sfr274I CTCGAG 2 cut(s) 43, 114
SinI GGWCC 1 cut(s) 858
SlaI CTCGAG 2 cut(s) 43, 114
SmiMI CAYNNNNRTG 1 cut(s) 845
SmlI CTYRAG 3 cut(s) 7, 43, 114
SmoI CTYRAG 3 cut(s) 7, 43, 114
Sse9I AATT 9 cut(s) 13, 199, 413, 576, 677, 682, 720, 827, 900
SsiI CCGC 5 cut(s) 91, 242, 496, 834, 861
SspI AATATT 1 cut(s) 765
StyD4I CCNGG 2 cut(s) 93, 231
TaaI ACNGT 3 cut(s) 355, 559, 847
TaqI TCGA 4 cut(s) 44, 115, 350, 399
TasI AATT 9 cut(s) 13, 199, 413, 576, 677, 682, 720, 827, 900
TatI WGTACW 3 cut(s) 3, 361, 875
TfiI GAWTC 1 cut(s) 531
Tru1I TTAA 2 cut(s) 786, 899
Tru9I TTAA 2 cut(s) 786, 899
TscAI CASTG 4 cut(s) 106, 127, 778, 850
TseFI GTSAC 1 cut(s) 235
TseI GCWGC 1 cut(s) 813
Tsp45I GTSAC 1 cut(s) 235
TspDTI ATGAA 4 cut(s) 16, 43, 58, 829
TspGWI ACGGA 1 cut(s) 385
TspRI CASTG 4 cut(s) 106, 127, 778, 850
Van91I CCANNNNNTGG 1 cut(s) 313
VpaK11BI GGWCC 1 cut(s) 858
XapI RAATTY 1 cut(s) 827
XhoI CTCGAG 2 cut(s) 43, 114
XmiI GTMKAC 1 cut(s) 443
XmnI GAANNNNTTC 2 cut(s) 23, 535
Zsp2I ATGCAT 1 cut(s) 341
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.