Prupe.4G282600_v2.0.a1

Acts as a Mg(2 ) transporter. Can also transport other divalent cations such as Fe(2 ), Sr(2 ), Ba(2 ), Mn(2 ) and Co(2 ) but to a much less extent than Mg(2 )

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0000984
Physical Location & Seq
Forward (+)
24177784 .. 24185467
7684 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G282600.3

Sequence Viewer

Length: 1035 bp
ATGTACTCGAGCAATTTGAAAGGCTTCATACTGGCTGTGGTCTCAAGCGCCTTCATCGGCTCCAGCTTCATCATCAAGAAGAAGGGTCTCAGGCGTGCCGGCGTCAATGGTCCTCGTGCAAGTGTCGGAGGATATGGGTATCTGTTGGAGCCCCTATGGTGGATAGGAATGATAACAATGGTTGTTGGAGAGATAGCCAATTTTGTAGCATACATTTACGCCCCTGCTGGGCTTGTGACGCCGCTTGGTGCATTGAGTATCATTGTTAGTGCTGTTTTAGCGCATTTCTTGTTAAAGGAGAAACTCCAGAAAATGGGAATGTTGGGGTGCCTTTTATGCATAGTGGGTTCGACTGTGATTGTACTCCATTCACCCAAAGAGCGTTCTCTCAGTTCTGTCGAAGAAATCTGGGAGTTAGCAATACAACCTGCATTTCTTTTGTATACAGCCTCGGTGGTAGCTGTAACGTTAGTCCTGATATTGTATTGTTCTCCCCGCTATGGACAAACCAATATACTGATTTATATAGGAATCTGCTCTATGATAGGATCATTGACAGTCATGAGTGTAAAAGCAATTGGCATTGCTATAAAACTCACATTAGAGGGTACAAACCAGCTAAAATACTTCCAAACATGGATTTTTGGAATGGTTGCAGTTACCTGCATCATCACTCAATTGAATTATCTAAACATGGCATTGGATACTTTCAACACAGCAATTGTTTCTCCTATCTATTATGCTATGTTCACCTCTTTTACAATATTTGCCAGTGCAATAATGTTTAAGGATTATTCTGGTCAAAGCGTGACCAGCATAGCGTCAGAACTTTGCGGGTTCATCACTGTGTTATCTGGGACTGCTATATTGCATAGTACACGAGAGCCAGATCCACCTTTAATTACAGATTTGTACACACCATTGTCTCCGAAGGTATCATGGTACATCCAAAGTAATGGGGAATGGAAGCAGAAGGATGAGGATGGTTCAACGCCTAATTTCATCACAATTCTTCGGCCAGACTACTTCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mg_trans_NIPA

Protein Analysis

345

Amino Acids

37.64

Weight (kDa)

8.61

Isoelectric Point (pI)

28.73

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 436, 671
AccB1I GGYRCC 1 cut(s) 327
AccB7I CCANNNNNTGG 1 cut(s) 313
AccI GTMKAC 1 cut(s) 443
AciI CCGC 3 cut(s) 242, 496, 834
AclI AACGTT 1 cut(s) 467
AclWI GGATC 2 cut(s) 556, 884
AcoI YGGCCR 1 cut(s) 1016
AcyI GRCGYC 2 cut(s) 102, 239
AfaI GTAC 6 cut(s) 5, 363, 610, 877, 914, 944
AfiI CCNNNNNNNGG 4 cut(s) 159, 228, 313, 500
AgsI TTSAA 5 cut(s) 19, 682, 712, 990, 1030
AluBI AGCT 3 cut(s) 66, 461, 619
AluI AGCT 3 cut(s) 66, 461, 619
Alw26I GTCTC 3 cut(s) 46, 92, 930
AlwI GGATC 2 cut(s) 556, 884
Ama87I CYCGRG 1 cut(s) 7
AoxI GGCC 1 cut(s) 1016
Asp700I GAANNNNTTC 1 cut(s) 23
AspLEI GCGC 2 cut(s) 50, 283
AspS9I GGNCC 1 cut(s) 110
AsuHPI GGTGA 2 cut(s) 363, 742
AvaI CYCGRG 1 cut(s) 7
AvaII GGWCC 1 cut(s) 110
BanI GGYRCC 1 cut(s) 327
BanII GRGCYC 1 cut(s) 153
BauI CACGAG 2 cut(s) 114, 879
BccI CCATC 1 cut(s) 977
BciVI GTATCC 1 cut(s) 697
BcoDI GTCTC 3 cut(s) 46, 92, 930
BfoI RGCGCY 1 cut(s) 51
BfuAI ACCTGC 2 cut(s) 436, 671
BfuI GTATCC 1 cut(s) 697
BisI GCNGC 1 cut(s) 242
BlsI GCNGC 1 cut(s) 243
Bme18I GGWCC 1 cut(s) 110
BmeT110I CYCGRG 1 cut(s) 7
BmgT120I GGNCC 1 cut(s) 110
BmiI GGNNCC 3 cut(s) 61, 150, 329
BmsI GCATC 1 cut(s) 675
BpmI CTGGAG 2 cut(s) 46, 290
BpuEI CTTGAG 1 cut(s) 28
BsaHI GRCGYC 2 cut(s) 102, 239
BsaI GGTCTC 2 cut(s) 46, 92
BsaJI CCNNGG 1 cut(s) 450
Bsc4I CCNNNNNNNGG 4 cut(s) 159, 228, 313, 500
Bse118I RCCGGY 1 cut(s) 98
Bse1I ACTGG 2 cut(s) 36, 771
Bse3DI GCAATG 1 cut(s) 582
BseDI CCNNGG 1 cut(s) 450
BseGI GGATG 3 cut(s) 945, 982, 988
BseLI CCNNNNNNNGG 4 cut(s) 159, 228, 313, 500
BseMI GCAATG 1 cut(s) 582
BseMII CTCAG 2 cut(s) 103, 403
BseNI ACTGG 2 cut(s) 36, 771
BseYI CCCAGC 1 cut(s) 227
BshFI GGCC 1 cut(s) 1018
BshNI GGYRCC 1 cut(s) 327
BsiHKCI CYCGRG 1 cut(s) 7
BsiSI CCGG 1 cut(s) 99
BslFI GGGAC 1 cut(s) 871
BslI CCNNNNNNNGG 4 cut(s) 159, 228, 313, 500
BsmAI GTCTC 3 cut(s) 46, 92, 930
BsmFI GGGAC 1 cut(s) 871
BsnI GGCC 1 cut(s) 1018
Bso31I GGTCTC 2 cut(s) 46, 92
BsoBI CYCGRG 1 cut(s) 7
Bsp1286I GDGCHC 1 cut(s) 153
Bsp1407I TGTACA 1 cut(s) 912
Bsp143I GATC 2 cut(s) 548, 889
BspACI CCGC 3 cut(s) 242, 496, 834
BspANI GGCC 1 cut(s) 1018
BspCNI CTCAG 2 cut(s) 102, 402
BspHI TCATGA 1 cut(s) 561
BspLI GGNNCC 3 cut(s) 61, 150, 329
BspMI ACCTGC 2 cut(s) 436, 671
BspPI GGATC 2 cut(s) 556, 884
BspT107I GGYRCC 1 cut(s) 327
BspTNI GGTCTC 2 cut(s) 46, 92
BsrDI GCAATG 1 cut(s) 582
BsrFI RCCGGY 1 cut(s) 98
BsrGI TGTACA 1 cut(s) 912
BsrI ACTGG 2 cut(s) 36, 771
BssAI RCCGGY 1 cut(s) 98
BssECI CCNNGG 1 cut(s) 450
BssMI GATC 2 cut(s) 548, 889
BssNAI GTATAC 1 cut(s) 444
BssNI GRCGYC 2 cut(s) 102, 239
BssSI CACGAG 2 cut(s) 114, 879
Bst1107I GTATAC 1 cut(s) 444
Bst2BI CACGAG 2 cut(s) 114, 879
Bst4CI ACNGT 3 cut(s) 355, 559, 847
BstACI GRCGYC 2 cut(s) 102, 239
BstAUI TGTACA 1 cut(s) 912
BstC8I GCNNGC 2 cut(s) 96, 100
BstDEI CTNAG 2 cut(s) 89, 389
BstF5I GGATG 3 cut(s) 945, 982, 988
BstH2I RGCGCY 1 cut(s) 51
BstHHI GCGC 2 cut(s) 50, 283
BstKTI GATC 2 cut(s) 551, 892
BstMAI GTCTC 3 cut(s) 46, 92, 930
BstMBI GATC 2 cut(s) 548, 889
BstMWI GCNNNNNNNGC 4 cut(s) 238, 278, 336, 813
BstX2I RGATCY 1 cut(s) 889
BstXI CCANNNNNNTGG 1 cut(s) 956
BstYI RGATCY 1 cut(s) 889
BstZ17I GTATAC 1 cut(s) 444
BsuI GTATCC 1 cut(s) 697
BsuRI GGCC 1 cut(s) 1018
BtsCI GGATG 3 cut(s) 945, 982, 988
BtsIMutI CAGTG 2 cut(s) 778, 843
BveI ACCTGC 2 cut(s) 436, 671
Cac8I GCNNGC 2 cut(s) 96, 100
CciI TCATGA 1 cut(s) 561
CfoI GCGC 2 cut(s) 50, 283
Cfr10I RCCGGY 1 cut(s) 98
Cfr13I GGNCC 1 cut(s) 110
CseI GACGC 3 cut(s) 91, 247, 810
Csp6I GTAC 6 cut(s) 4, 362, 609, 876, 913, 943
CviAII CATG 4 cut(s) 562, 636, 694, 939
CviQI GTAC 6 cut(s) 4, 362, 609, 876, 913, 943
DdeI CTNAG 2 cut(s) 89, 389
DpnI GATC 2 cut(s) 550, 891
DpnII GATC 2 cut(s) 548, 889
EaeI YGGCCR 1 cut(s) 1016
Eco24I GRGCYC 1 cut(s) 153
Eco31I GGTCTC 2 cut(s) 46, 92
Eco47I GGWCC 1 cut(s) 110
Eco88I CYCGRG 1 cut(s) 7
EcoT22I ATGCAT 1 cut(s) 341
EcoT38I GRGCYC 1 cut(s) 153
FaeI CATG 4 cut(s) 565, 639, 697, 942
FaqI GGGAC 1 cut(s) 871
FatI CATG 4 cut(s) 561, 635, 693, 938
FauI CCCGC 2 cut(s) 503, 827
FblI GTMKAC 1 cut(s) 443
Fnu4HI GCNGC 1 cut(s) 242
FokI GGATG 3 cut(s) 932, 989, 995
FriOI GRGCYC 1 cut(s) 153
Fsp4HI GCNGC 1 cut(s) 242
GlaI GCGC 2 cut(s) 49, 282
GluI GCNGC 1 cut(s) 242
GsaI CCCAGC 1 cut(s) 231
GsuI CTGGAG 2 cut(s) 46, 290
HaeII RGCGCY 1 cut(s) 51
HaeIII GGCC 1 cut(s) 1018
HapII CCGG 1 cut(s) 99
HgaI GACGC 3 cut(s) 91, 247, 810
HhaI GCGC 2 cut(s) 50, 283
Hin1I GRCGYC 2 cut(s) 102, 239
Hin1II CATG 4 cut(s) 565, 639, 697, 942
Hin6I GCGC 2 cut(s) 48, 281
HinP1I GCGC 2 cut(s) 48, 281
HinfI GANTC 1 cut(s) 531
HpaII CCGG 1 cut(s) 99
HphI GGTGA 2 cut(s) 363, 742
Hpy166II GTNNAC 4 cut(s) 444, 750, 878, 915
Hpy188I TCNGA 3 cut(s) 128, 826, 930
Hpy188III TCNNGA 4 cut(s) 76, 307, 475, 562
Hpy8I GTNNAC 4 cut(s) 444, 750, 878, 915
HpyAV CCTTC 4 cut(s) 61, 76, 925, 967
HpyCH4III ACNGT 3 cut(s) 355, 559, 847
HpyCH4IV ACGT 1 cut(s) 467
HpyCH4V TGCA 8 cut(s) 119, 251, 339, 431, 656, 666, 776, 871
HpyF10VI GCNNNNNNNGC 4 cut(s) 238, 278, 336, 813
HpyF3I CTNAG 2 cut(s) 89, 389
HpySE526I ACGT 1 cut(s) 467
Hsp92I GRCGYC 2 cut(s) 102, 239
Hsp92II CATG 4 cut(s) 565, 639, 697, 942
HspAI GCGC 2 cut(s) 48, 281
KroI GCCGGC 1 cut(s) 98
KroNI GCCGGC 1 cut(s) 100
Kzo9I GATC 2 cut(s) 548, 889
LmnI GCTCC 2 cut(s) 65, 148
LweI GCATC 1 cut(s) 675
MaeII ACGT 1 cut(s) 467
MaeIII GTNAC 4 cut(s) 235, 463, 658, 808
MalI GATC 2 cut(s) 550, 891
MboI GATC 2 cut(s) 548, 889
MboII GAAGA 3 cut(s) 91, 413, 1004
MfeI CAATTG 3 cut(s) 576, 677, 720
MflI RGATCY 1 cut(s) 889
MhlI GDGCHC 1 cut(s) 153
MluCI AATT 9 cut(s) 13, 199, 576, 677, 682, 720, 900, 997, 1008
MmeI TCCRAC 3 cut(s) 106, 126, 166
MnlI CCTC 6 cut(s) 122, 123, 460, 598, 763, 973
Mph1103I ATGCAT 1 cut(s) 341
MroNI GCCGGC 1 cut(s) 98
MroXI GAANNNNTTC 1 cut(s) 23
MseI TTAA 3 cut(s) 293, 786, 899
MslI CAYNNNNRTG 1 cut(s) 845
MspI CCGG 1 cut(s) 99
MunI CAATTG 3 cut(s) 576, 677, 720
MwoI GCNNNNNNNGC 4 cut(s) 238, 278, 336, 813
NaeI GCCGGC 1 cut(s) 100
NdeII GATC 2 cut(s) 548, 889
NgoMIV GCCGGC 1 cut(s) 98
NlaIII CATG 4 cut(s) 565, 639, 697, 942
NlaIV GGNNCC 3 cut(s) 61, 150, 329
NmuCI GTSAC 2 cut(s) 235, 808
NsiI ATGCAT 1 cut(s) 341
PaeR7I CTCGAG 1 cut(s) 7
PagI TCATGA 1 cut(s) 561
PdiI GCCGGC 1 cut(s) 100
PdmI GAANNNNTTC 1 cut(s) 23
PfeI GAWTC 1 cut(s) 531
PflMI CCANNNNNTGG 1 cut(s) 313
PkrI GCNGC 1 cut(s) 243
Psp1406I AACGTT 1 cut(s) 467
PspFI CCCAGC 1 cut(s) 227
PspN4I GGNNCC 3 cut(s) 61, 150, 329
PspPI GGNCC 1 cut(s) 110
PspXI VCTCGAGB 1 cut(s) 7
PsuI RGATCY 1 cut(s) 889
RsaI GTAC 6 cut(s) 5, 363, 610, 877, 914, 944
RsaNI GTAC 6 cut(s) 4, 362, 609, 876, 913, 943
RseI CAYNNNNRTG 1 cut(s) 845
SaqAI TTAA 3 cut(s) 293, 786, 899
SatI GCNGC 1 cut(s) 242
Sau3AI GATC 2 cut(s) 548, 889
Sau96I GGNCC 1 cut(s) 110
SduI GDGCHC 1 cut(s) 153
SetI ASST 9 cut(s) 68, 430, 463, 470, 621, 665, 755, 898, 936
SfaNI GCATC 1 cut(s) 675
Sfr274I CTCGAG 1 cut(s) 7
SinI GGWCC 1 cut(s) 110
SlaI CTCGAG 1 cut(s) 7
SmiMI CAYNNNNRTG 1 cut(s) 845
SmlI CTYRAG 2 cut(s) 7, 43
SmoI CTYRAG 2 cut(s) 7, 43
Sse9I AATT 9 cut(s) 13, 199, 576, 677, 682, 720, 900, 997, 1008
SsiI CCGC 3 cut(s) 242, 496, 834
SspI AATATT 1 cut(s) 765
TaaI ACNGT 3 cut(s) 355, 559, 847
TaiI ACGT 1 cut(s) 470
TaqI TCGA 3 cut(s) 8, 350, 399
TasI AATT 9 cut(s) 13, 199, 576, 677, 682, 720, 900, 997, 1008
TatI WGTACW 4 cut(s) 3, 361, 875, 912
TauI GCSGC 1 cut(s) 244
TfiI GAWTC 1 cut(s) 531
Tru1I TTAA 3 cut(s) 293, 786, 899
Tru9I TTAA 3 cut(s) 293, 786, 899
TscAI CASTG 2 cut(s) 778, 850
TseFI GTSAC 2 cut(s) 235, 808
Tsp45I GTSAC 2 cut(s) 235, 808
TspDTI ATGAA 5 cut(s) 16, 43, 58, 829, 991
TspRI CASTG 2 cut(s) 778, 850
Van91I CCANNNNNTGG 1 cut(s) 313
VpaK11BI GGWCC 1 cut(s) 110
XhoI CTCGAG 1 cut(s) 7
XmiI GTMKAC 1 cut(s) 443
XmnI GAANNNNTTC 1 cut(s) 23
Zsp2I ATGCAT 1 cut(s) 341
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.