pycom15g12590

Acts as a Mg(2 ) transporter. Can also transport other divalent cations such as Fe(2 ), Sr(2 ), Ba(2 ), Mn(2 ) and Co(2 ) but to a much less extent than Mg(2 )

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0000984
Physical Location & Seq
Forward (+)
8600948 .. 8604258
3311 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g12590.5

Sequence Viewer

Length: 1206 bp
ATGTACTCGAGCAATTTGAAAGGCTTCATCTTGGCCGTCGTCTCGAGCGCCTTCATTGGCTCCAGCTTCATCTTCAAGAAGAAGGGTCTCATCCGGGCCAGCGTCAACGGCCCTGCTGCCAGTGTGGGAGGATATGGGTATCTGAAGGAGCCTCTATGGTGGATAGGAATGGTAACAATGGTTATTGGAGAGCTAGCCAATTTTGTAGCATACGTTTACGCACCTGCTGTCCTTGTAACACCACTAGGTGCATTGAGTATTATTGTTAGTGCTGTTTTAGCGCATTTTTTGTTAAAGGAGAAACTCCAGAAAATGGGAATGTTGGGGTGTCTTTTATGTATAGTGGGTTCAACTGTGATTGTACTCCATTCACCTGAAGAGCAATCTCTCAGTTCGGTCCAAGAAATCTGGGAATTAGCAATACAACCTGCATTTCTTTTGTATACAGCCTCAGTGGTAGCTGTGATGTTAGTACTGATGTTGTACTGCGCTCCCCGCTATGGCCAAACTAATATACTGATTTATATAGGAATCTGCTCTATAATAGGATCATTAACAGTTATGAGTGTAAAAGCAATTGGGATTGCAATAAAACTCACATTAGAGGGCACAAACCAGCTAAAATACTTCCAAACATGGATTTTTGGAATGGTTGCAGTTACCTGTATCATCACTCAATTAAATTATTTAAACATGGTCCGTATCTTGTTATGCTCTATTACATTGCATCATGGCAGCTTTGTGTCCTTGTCATTTCTATTTTTGGAATGGTTGCAGTTACCTATATTATCATTCTATAAAACTCACATTAGAGGGCACAAACCAGCTAATATACTTCCAAACATGGATTTTTGGAATGGTTTCAGTTACCTGTATCATCACTCAATTGAATTATCTAAACATGGTCTTGTCTTGGTATGCTCTATTACATTGCATCATGACAGCTTTGCGTTGGATACTTTCAACACAGCAATTGTTTCTCCCATCTATTATGCTATGTTCACATCTTTCACAATATTTGCCAGTGCAATAATGTTTAAGGATTATTCGGGTCAAAGTGTGAGCAGCATAGCATCAGAACTTTGCGGTTTCATCACTGTGTTATCTGGGACTGCTATATTGCATAGTACAAGAGAGCCAGATCCACCTTTAATTACAGGTTTGTATCTTGTGTATCTGATTGCATGTCTATTTGGAGATTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mg_trans_NIPA

Protein Analysis

402

Amino Acids

44.03

Weight (kDa)

8.02

Isoelectric Point (pI)

31.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 232
Acc36I ACCTGC 2 cut(s) 232, 436
AccB7I CCANNNNNTGG 1 cut(s) 313
AccI GTMKAC 1 cut(s) 443
AciI CCGC 2 cut(s) 496, 1086
AclWI GGATC 2 cut(s) 556, 1136
AcoI YGGCCR 2 cut(s) 33, 502
AcuI CTGAAG 2 cut(s) 164, 396
AdeI CACNNNGTG 1 cut(s) 248
AfaI GTAC 5 cut(s) 5, 363, 474, 485, 1129
AfiI CCNNNNNNNGG 2 cut(s) 313, 500
AgsI TTSAA 5 cut(s) 19, 76, 351, 890, 964
AluBI AGCT 7 cut(s) 66, 193, 461, 619, 738, 827, 945
AluI AGCT 7 cut(s) 66, 193, 461, 619, 738, 827, 945
Alw26I GTCTC 2 cut(s) 46, 92
AlwI GGATC 2 cut(s) 556, 1136
Ama87I CYCGRG 2 cut(s) 7, 43
AoxI GGCC 4 cut(s) 33, 96, 109, 502
ApeKI GCWGC 3 cut(s) 116, 735, 1065
Asp700I GAANNNNTTC 2 cut(s) 23, 860
AspLEI GCGC 3 cut(s) 50, 283, 491
AspS9I GGNCC 4 cut(s) 96, 110, 397, 697
AsuC2I CCSGG 1 cut(s) 95
AsuHPI GGTGA 1 cut(s) 363
AsuNHI GCTAGC 1 cut(s) 193
AvaI CYCGRG 2 cut(s) 7, 43
AvaII GGWCC 2 cut(s) 397, 697
BaeGI GKGCMC 2 cut(s) 611, 819
BalI TGGCCA 1 cut(s) 504
BbvI GCAGC 3 cut(s) 103, 747, 1077
BccI CCATC 1 cut(s) 992
BceAI ACGGC 2 cut(s) 20, 124
BciVI GTATCC 1 cut(s) 949
BcnI CCSGG 1 cut(s) 95
BcoDI GTCTC 2 cut(s) 46, 92
BfaI CTAG 2 cut(s) 194, 245
BfoI RGCGCY 1 cut(s) 51
BfuAI ACCTGC 2 cut(s) 232, 436
BfuI GTATCC 1 cut(s) 949
BisI GCNGC 3 cut(s) 117, 736, 1066
BlsI GCNGC 3 cut(s) 118, 737, 1067
BmcAI AGTACT 1 cut(s) 474
Bme1390I CCNGG 1 cut(s) 95
Bme18I GGWCC 2 cut(s) 397, 697
BmeT110I CYCGRG 2 cut(s) 7, 43
BmgT120I GGNCC 4 cut(s) 96, 110, 397, 697
BmiI GGNNCC 2 cut(s) 61, 150
BmrFI CCNGG 1 cut(s) 95
BmsI GCATC 3 cut(s) 736, 943, 1082
BmtI GCTAGC 1 cut(s) 197
BpmI CTGGAG 2 cut(s) 46, 290
BpuMI CCSGG 1 cut(s) 95
BsaI GGTCTC 1 cut(s) 92
Bsc4I CCNNNNNNNGG 2 cut(s) 313, 500
Bse1I ACTGG 2 cut(s) 120, 1023
Bse3DI GCAATG 2 cut(s) 722, 929
BseGI GGATG 1 cut(s) 90
BseLI CCNNNNNNNGG 2 cut(s) 313, 500
BseMI GCAATG 2 cut(s) 722, 929
BseMII CTCAG 2 cut(s) 403, 465
BseNI ACTGG 2 cut(s) 120, 1023
BseSI GKGCMC 2 cut(s) 611, 819
BseXI GCAGC 3 cut(s) 103, 747, 1077
BshFI GGCC 4 cut(s) 35, 98, 111, 504
BsiHKCI CYCGRG 2 cut(s) 7, 43
BsiSI CCGG 1 cut(s) 94
BslFI GGGAC 1 cut(s) 1123
BslI CCNNNNNNNGG 2 cut(s) 313, 500
BsmAI GTCTC 2 cut(s) 46, 92
BsmBI CGTCTC 1 cut(s) 46
BsmFI GGGAC 1 cut(s) 1123
BsnI GGCC 4 cut(s) 35, 98, 111, 504
Bso31I GGTCTC 1 cut(s) 92
BsoBI CYCGRG 2 cut(s) 7, 43
Bsp1286I GDGCHC 2 cut(s) 611, 819
Bsp143I GATC 2 cut(s) 548, 1141
BspACI CCGC 2 cut(s) 496, 1086
BspANI GGCC 4 cut(s) 35, 98, 111, 504
BspCNI CTCAG 2 cut(s) 402, 464
BspHI TCATGA 1 cut(s) 937
BspLI GGNNCC 2 cut(s) 61, 150
BspMI ACCTGC 2 cut(s) 232, 436
BspOI GCTAGC 1 cut(s) 197
BspPI GGATC 2 cut(s) 556, 1136
BspQI GCTCTTC 1 cut(s) 372
BspTNI GGTCTC 1 cut(s) 92
BsrDI GCAATG 2 cut(s) 722, 929
BsrI ACTGG 2 cut(s) 120, 1023
BssMI GATC 2 cut(s) 548, 1141
BssNAI GTATAC 1 cut(s) 444
Bst1107I GTATAC 1 cut(s) 444
Bst4CI ACNGT 3 cut(s) 355, 559, 1099
Bst6I CTCTTC 1 cut(s) 372
BstC8I GCNNGC 2 cut(s) 100, 195
BstDEI CTNAG 2 cut(s) 389, 451
BstF5I GGATG 1 cut(s) 90
BstH2I RGCGCY 1 cut(s) 51
BstHHI GCGC 3 cut(s) 50, 283, 491
BstKTI GATC 2 cut(s) 551, 1144
BstMAI GTCTC 2 cut(s) 46, 92
BstMBI GATC 2 cut(s) 548, 1141
BstMWI GCNNNNNNNGC 3 cut(s) 108, 278, 495
BstNSI RCATGY 1 cut(s) 1188
BstSCI CCNGG 1 cut(s) 93
BstSLI GKGCMC 2 cut(s) 611, 819
BstV1I GCAGC 3 cut(s) 103, 747, 1077
BstX2I RGATCY 1 cut(s) 1141
BstYI RGATCY 1 cut(s) 1141
BstZ17I GTATAC 1 cut(s) 444
BsuI GTATCC 1 cut(s) 949
BsuRI GGCC 4 cut(s) 35, 98, 111, 504
BtsCI GGATG 1 cut(s) 90
BtsIMutI CAGTG 4 cut(s) 127, 459, 1030, 1095
BveI ACCTGC 2 cut(s) 232, 436
Cac8I GCNNGC 2 cut(s) 100, 195
CciI TCATGA 1 cut(s) 937
CfoI GCGC 3 cut(s) 50, 283, 491
Cfr13I GGNCC 4 cut(s) 96, 110, 397, 697
CseI GACGC 1 cut(s) 91
Csp6I GTAC 5 cut(s) 4, 362, 473, 484, 1128
CviAII CATG 7 cut(s) 636, 694, 731, 844, 902, 938, 1185
CviQI GTAC 5 cut(s) 4, 362, 473, 484, 1128
DdeI CTNAG 2 cut(s) 389, 451
DpnI GATC 2 cut(s) 550, 1143
DpnII GATC 2 cut(s) 548, 1141
DraI TTTAAA 1 cut(s) 690
DraIII CACNNNGTG 1 cut(s) 248
EaeI YGGCCR 2 cut(s) 33, 502
Eam1104I CTCTTC 1 cut(s) 372
EarI CTCTTC 1 cut(s) 372
Eco31I GGTCTC 1 cut(s) 92
Eco47I GGWCC 2 cut(s) 397, 697
Eco57I CTGAAG 2 cut(s) 164, 396
Eco88I CYCGRG 2 cut(s) 7, 43
Esp3I CGTCTC 1 cut(s) 46
FaeI CATG 7 cut(s) 639, 697, 734, 847, 905, 941, 1188
FaqI GGGAC 1 cut(s) 1123
FatI CATG 7 cut(s) 635, 693, 730, 843, 901, 937, 1184
FauI CCCGC 1 cut(s) 503
FblI GTMKAC 1 cut(s) 443
Fnu4HI GCNGC 3 cut(s) 117, 736, 1066
FokI GGATG 1 cut(s) 77
Fsp4HI GCNGC 3 cut(s) 117, 736, 1066
FspBI CTAG 2 cut(s) 194, 245
GlaI GCGC 3 cut(s) 49, 282, 490
GluI GCNGC 3 cut(s) 117, 736, 1066
GsuI CTGGAG 2 cut(s) 46, 290
HaeII RGCGCY 1 cut(s) 51
HaeIII GGCC 4 cut(s) 35, 98, 111, 504
HapII CCGG 1 cut(s) 94
HgaI GACGC 1 cut(s) 91
HhaI GCGC 3 cut(s) 50, 283, 491
Hin1II CATG 7 cut(s) 639, 697, 734, 847, 905, 941, 1188
Hin6I GCGC 3 cut(s) 48, 281, 489
HinP1I GCGC 3 cut(s) 48, 281, 489
HincII GTYRAC 1 cut(s) 106
HindII GTYRAC 1 cut(s) 106
HinfI GANTC 2 cut(s) 531, 1199
HpaII CCGG 1 cut(s) 94
HphI GGTGA 1 cut(s) 363
Hpy166II GTNNAC 4 cut(s) 106, 217, 444, 1002
Hpy188I TCNGA 3 cut(s) 144, 1078, 1179
Hpy188III TCNNGA 4 cut(s) 43, 76, 307, 938
Hpy8I GTNNAC 4 cut(s) 106, 217, 444, 1002
Hpy99I CGWCG 1 cut(s) 41
HpyAV CCTTC 3 cut(s) 61, 76, 139
HpyCH4III ACNGT 3 cut(s) 355, 559, 1099
HpyCH4IV ACGT 1 cut(s) 213
HpyF10VI GCNNNNNNNGC 3 cut(s) 108, 278, 495
HpyF3I CTNAG 2 cut(s) 389, 451
HpySE526I ACGT 1 cut(s) 213
Hsp92II CATG 7 cut(s) 639, 697, 734, 847, 905, 941, 1188
HspAI GCGC 3 cut(s) 48, 281, 489
Kzo9I GATC 2 cut(s) 548, 1141
LguI GCTCTTC 1 cut(s) 372
LmnI GCTCC 3 cut(s) 65, 148, 496
Lsp1109I GCAGC 3 cut(s) 103, 747, 1077
LweI GCATC 3 cut(s) 736, 943, 1082
MaeI CTAG 2 cut(s) 194, 245
MaeII ACGT 1 cut(s) 213
MaeIII GTNAC 5 cut(s) 172, 235, 658, 777, 866
MalI GATC 2 cut(s) 550, 1143
MboI GATC 2 cut(s) 548, 1141
MboII GAAGA 3 cut(s) 64, 91, 389
MfeI CAATTG 3 cut(s) 576, 885, 972
MflI RGATCY 1 cut(s) 1141
MhlI GDGCHC 2 cut(s) 611, 819
MlsI TGGCCA 1 cut(s) 504
MluNI TGGCCA 1 cut(s) 504
MmeI TCCRAC 1 cut(s) 933
MnlI CCTC 5 cut(s) 122, 162, 460, 598, 806
Mox20I TGGCCA 1 cut(s) 504
MroXI GAANNNNTTC 2 cut(s) 23, 860
MscI TGGCCA 1 cut(s) 504
MseI TTAA 7 cut(s) 293, 554, 680, 689, 1038, 1151, 1204
MslI CAYNNNNRTG 1 cut(s) 1097
Msp20I TGGCCA 1 cut(s) 504
MspI CCGG 1 cut(s) 94
MspR9I CCNGG 1 cut(s) 95
MunI CAATTG 3 cut(s) 576, 885, 972
MwoI GCNNNNNNNGC 3 cut(s) 108, 278, 495
NciI CCSGG 1 cut(s) 95
NdeII GATC 2 cut(s) 548, 1141
NheI GCTAGC 1 cut(s) 193
NlaIII CATG 7 cut(s) 639, 697, 734, 847, 905, 941, 1188
NlaIV GGNNCC 2 cut(s) 61, 150
NspI RCATGY 1 cut(s) 1188
PaeR7I CTCGAG 2 cut(s) 7, 43
PagI TCATGA 1 cut(s) 937
PaqCI CACCTGC 1 cut(s) 232
PciSI GCTCTTC 1 cut(s) 372
PdmI GAANNNNTTC 2 cut(s) 23, 860
PfeI GAWTC 2 cut(s) 531, 1199
PflMI CCANNNNNTGG 1 cut(s) 313
PkrI GCNGC 3 cut(s) 118, 737, 1067
PspN4I GGNNCC 2 cut(s) 61, 150
PspPI GGNCC 4 cut(s) 96, 110, 397, 697
PspXI VCTCGAGB 1 cut(s) 7
PsrI GAACNNNNNNTAC 2 cut(s) 331, 363
PsuI RGATCY 1 cut(s) 1141
RsaI GTAC 5 cut(s) 5, 363, 474, 485, 1129
RsaNI GTAC 5 cut(s) 4, 362, 473, 484, 1128
RseI CAYNNNNRTG 1 cut(s) 1097
SapI GCTCTTC 1 cut(s) 372
SaqAI TTAA 7 cut(s) 293, 554, 680, 689, 1038, 1151, 1204
SatI GCNGC 3 cut(s) 117, 736, 1066
Sau3AI GATC 2 cut(s) 548, 1141
Sau96I GGNCC 4 cut(s) 96, 110, 397, 697
ScaI AGTACT 1 cut(s) 474
ScrFI CCNGG 1 cut(s) 95
SduI GDGCHC 2 cut(s) 611, 819
SfaNI GCATC 3 cut(s) 736, 943, 1082
Sfr274I CTCGAG 2 cut(s) 7, 43
SinI GGWCC 2 cut(s) 397, 697
SlaI CTCGAG 2 cut(s) 7, 43
SmiMI CAYNNNNRTG 1 cut(s) 1097
SmlI CTYRAG 2 cut(s) 7, 43
SmoI CTYRAG 2 cut(s) 7, 43
SsiI CCGC 2 cut(s) 496, 1086
SspI AATATT 1 cut(s) 1017
SspMI CTAG 2 cut(s) 194, 245
StyD4I CCNGG 1 cut(s) 93
TaaI ACNGT 3 cut(s) 355, 559, 1099
TaiI ACGT 1 cut(s) 216
TaqI TCGA 2 cut(s) 8, 44
TaqII GACCGA 1 cut(s) 385
TatI WGTACW 5 cut(s) 3, 361, 472, 483, 1127
TfiI GAWTC 2 cut(s) 531, 1199
Tru1I TTAA 7 cut(s) 293, 554, 680, 689, 1038, 1151, 1204
Tru9I TTAA 7 cut(s) 293, 554, 680, 689, 1038, 1151, 1204
TscAI CASTG 4 cut(s) 127, 459, 1030, 1102
TseI GCWGC 3 cut(s) 116, 735, 1065
TspDTI ATGAA 4 cut(s) 16, 43, 58, 1081
TspGWI ACGGA 1 cut(s) 689
TspRI CASTG 4 cut(s) 127, 459, 1030, 1102
Van91I CCANNNNNTGG 1 cut(s) 313
VpaK11BI GGWCC 2 cut(s) 397, 697
XceI RCATGY 1 cut(s) 1188
XhoI CTCGAG 2 cut(s) 7, 43
XmiI GTMKAC 1 cut(s) 443
XmnI GAANNNNTTC 2 cut(s) 23, 860
XspI CTAG 2 cut(s) 194, 245
ZrmI AGTACT 1 cut(s) 474
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.