MD15G1139500.v1.1

Acts as a Mg(2 ) transporter. Can also transport other divalent cations such as Fe(2 ), Sr(2 ), Ba(2 ), Mn(2 ) and Co(2 ) but to a much less extent than Mg(2 )

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000984
Physical Location & Seq
Forward (+)
10220670 .. 10226299
5630 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1139500.v1.1.491

Sequence Viewer

Length: 1035 bp
ATGTACTCGAGCAATTTGAAGGGCTTCATCTTGGCCGTCGTCTCGAGCGCCTTCATTGGCTCCAGCTTCATCATCAAGAAGAAGGGTCTCATCCGGGCCAGCGTCAACGGCCCTGCTGCCAGTGTGGGAGGATATGGGTATCTGAAGGAGTCTCTATGGTGGATAGGAATGGTAACAATGGTTATTGGAGAGATGGCCAATTTGGTAGCATACGTTTACGCACCTGCTGTCCTTGTAACACCACTAGGTGCATTGAGTATTATTGTTAGTGCTGTTTTAGCGCATTTTTTGTTAAAGGAGAAACTCCAGAAAATGGGAATGTTGGGGTGTCTTTTATGTATAGTGGGTTCAACTGAGATTGTACTCCATTCACCTGAAGAGCAATCTCTCAGTTCGGTCCAAGAAATCTGGGAATTAGCAATACAACCTGCATTTCTTTTGTATACAGCCTCGGTGGTAGCTGTGACATTAGTACTGATGTTGTACTGTGCTCCCCGCCATGGCCAAACCAATATACTGATTTATATAGGAATCTGCTCTATAATAGGATCATTAACAGTTATGAGTGTAAAAGCAATTGGGATTGCAATAAAACTCACATTTGAGGGTACAAACCAGCTAAAATACTTCCAAACATGGATTTTTGGAATGGTTGCAGTTACCTGTATCATCACTCAATTAAATTATCTAAACATGGCGTTGGATACTTTCAACACAGCAATTGTTTCTCCGATCTATTATGCTATGTTCACATCTTTTACAATGCTTGCCAGTGCAATAATGTTTAAGGATTATTCGGGTCAAAGTGTGAGCAGCATAGCATCAGAACTTTGTGGTTTCATCACTGTGTTATCTGGGACTGTTATATTGCATAGTACAAGAGAGCCAGATCCACCTTTAATTACAGATTTGTACACACCATTATCTCCGAAAGTATCATGGTACATACAAAGCAACGGGGAATGGAAGCAGAAGGATGAGGATGGCTCAACCCCTAATTTCCTCACAGTTCTTCGGCAAGACTACTTCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mg_trans_NIPA

Protein Analysis

345

Amino Acids

37.57

Weight (kDa)

6.88

Isoelectric Point (pI)

30.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mg_trans_NIPA PF05653 5 - 295 1.3e-129 Magnesium transporter NIPA
EamA PF00892 55 - 118 7.7e-07 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 232
Acc36I ACCTGC 2 cut(s) 232, 436
AccB7I CCANNNNNTGG 1 cut(s) 313
AccI GTMKAC 1 cut(s) 443
AciI CCGC 1 cut(s) 496
AclWI GGATC 2 cut(s) 556, 884
AcoI YGGCCR 3 cut(s) 33, 195, 502
AcuI CTGAAG 2 cut(s) 164, 396
AdeI CACNNNGTG 1 cut(s) 248
AfaI GTAC 8 cut(s) 5, 363, 474, 485, 610, 877, 914, 944
AfiI CCNNNNNNNGG 2 cut(s) 313, 500
AgsI TTSAA 4 cut(s) 19, 351, 712, 1030
AluBI AGCT 3 cut(s) 66, 461, 619
AluI AGCT 3 cut(s) 66, 461, 619
Alw21I GWGCWC 1 cut(s) 493
Alw26I GTCTC 3 cut(s) 46, 92, 156
AlwI GGATC 2 cut(s) 556, 884
Ama87I CYCGRG 2 cut(s) 7, 43
AoxI GGCC 5 cut(s) 33, 96, 109, 195, 502
ApeKI GCWGC 2 cut(s) 116, 813
Asp700I GAANNNNTTC 1 cut(s) 23
AspLEI GCGC 2 cut(s) 50, 283
AspS9I GGNCC 3 cut(s) 96, 110, 397
AsuC2I CCSGG 1 cut(s) 95
AsuHPI GGTGA 1 cut(s) 363
AvaI CYCGRG 2 cut(s) 7, 43
AvaII GGWCC 1 cut(s) 397
BalI TGGCCA 2 cut(s) 197, 504
Bbv12I GWGCWC 1 cut(s) 493
BbvI GCAGC 2 cut(s) 103, 825
BccI CCATC 2 cut(s) 187, 977
BceAI ACGGC 2 cut(s) 20, 124
BciVI GTATCC 1 cut(s) 697
BcnI CCSGG 1 cut(s) 95
BcoDI GTCTC 3 cut(s) 46, 92, 156
BfaI CTAG 1 cut(s) 245
BfoI RGCGCY 1 cut(s) 51
BfuAI ACCTGC 2 cut(s) 232, 436
BfuI GTATCC 1 cut(s) 697
BisI GCNGC 2 cut(s) 117, 814
BlsI GCNGC 2 cut(s) 118, 815
BmcAI AGTACT 1 cut(s) 474
Bme1390I CCNGG 1 cut(s) 95
Bme18I GGWCC 1 cut(s) 397
BmeT110I CYCGRG 2 cut(s) 7, 43
BmgT120I GGNCC 3 cut(s) 96, 110, 397
BmiI GGNNCC 1 cut(s) 61
BmrFI CCNGG 1 cut(s) 95
BmsI GCATC 1 cut(s) 830
BplI GAGNNNNNCTC 2 cut(s) 971, 1003
BpmI CTGGAG 2 cut(s) 46, 290
BpuMI CCSGG 1 cut(s) 95
BsaI GGTCTC 1 cut(s) 92
BsaJI CCNNGG 2 cut(s) 450, 499
Bsc4I CCNNNNNNNGG 2 cut(s) 313, 500
Bse1I ACTGG 2 cut(s) 120, 771
BseDI CCNNGG 2 cut(s) 450, 499
BseGI GGATG 3 cut(s) 90, 982, 988
BseLI CCNNNNNNNGG 2 cut(s) 313, 500
BseMII CTCAG 2 cut(s) 345, 403
BseNI ACTGG 2 cut(s) 120, 771
BseXI GCAGC 2 cut(s) 103, 825
BshFI GGCC 5 cut(s) 35, 98, 111, 197, 504
BsiHKAI GWGCWC 1 cut(s) 493
BsiHKCI CYCGRG 2 cut(s) 7, 43
BsiSI CCGG 1 cut(s) 94
BslFI GGGAC 1 cut(s) 871
BslI CCNNNNNNNGG 2 cut(s) 313, 500
BsmAI GTCTC 3 cut(s) 46, 92, 156
BsmBI CGTCTC 1 cut(s) 46
BsmFI GGGAC 1 cut(s) 871
BsnI GGCC 5 cut(s) 35, 98, 111, 197, 504
Bso31I GGTCTC 1 cut(s) 92
BsoBI CYCGRG 2 cut(s) 7, 43
Bsp1286I GDGCHC 1 cut(s) 493
Bsp1407I TGTACA 1 cut(s) 912
Bsp143I GATC 3 cut(s) 548, 732, 889
Bsp19I CCATGG 1 cut(s) 499
BspACI CCGC 1 cut(s) 496
BspANI GGCC 5 cut(s) 35, 98, 111, 197, 504
BspCNI CTCAG 2 cut(s) 346, 402
BspLI GGNNCC 1 cut(s) 61
BspMI ACCTGC 2 cut(s) 232, 436
BspPI GGATC 2 cut(s) 556, 884
BspQI GCTCTTC 1 cut(s) 372
BspTNI GGTCTC 1 cut(s) 92
BsrGI TGTACA 1 cut(s) 912
BsrI ACTGG 2 cut(s) 120, 771
BssECI CCNNGG 2 cut(s) 450, 499
BssMI GATC 3 cut(s) 548, 732, 889
BssNAI GTATAC 1 cut(s) 444
BssT1I CCWWGG 1 cut(s) 499
Bst1107I GTATAC 1 cut(s) 444
Bst4CI ACNGT 5 cut(s) 488, 559, 847, 862, 1009
Bst6I CTCTTC 1 cut(s) 372
BstAUI TGTACA 1 cut(s) 912
BstC8I GCNNGC 2 cut(s) 100, 768
BstDEI CTNAG 2 cut(s) 354, 389
BstDSI CCRYGG 1 cut(s) 499
BstF5I GGATG 3 cut(s) 90, 982, 988
BstH2I RGCGCY 1 cut(s) 51
BstHHI GCGC 2 cut(s) 50, 283
BstKTI GATC 3 cut(s) 551, 735, 892
BstMAI GTCTC 3 cut(s) 46, 92, 156
BstMBI GATC 3 cut(s) 548, 732, 889
BstMWI GCNNNNNNNGC 2 cut(s) 108, 278
BstSCI CCNGG 1 cut(s) 93
BstV1I GCAGC 2 cut(s) 103, 825
BstX2I RGATCY 1 cut(s) 889
BstYI RGATCY 1 cut(s) 889
BstZ17I GTATAC 1 cut(s) 444
BsuI GTATCC 1 cut(s) 697
BsuRI GGCC 5 cut(s) 35, 98, 111, 197, 504
BtgI CCRYGG 1 cut(s) 499
BtsCI GGATG 3 cut(s) 90, 982, 988
BtsIMutI CAGTG 3 cut(s) 127, 778, 843
BveI ACCTGC 2 cut(s) 232, 436
Cac8I GCNNGC 2 cut(s) 100, 768
CfoI GCGC 2 cut(s) 50, 283
Cfr13I GGNCC 3 cut(s) 96, 110, 397
CseI GACGC 1 cut(s) 91
Csp6I GTAC 8 cut(s) 4, 362, 473, 484, 609, 876, 913, 943
CviAII CATG 4 cut(s) 500, 636, 694, 939
CviQI GTAC 8 cut(s) 4, 362, 473, 484, 609, 876, 913, 943
DdeI CTNAG 2 cut(s) 354, 389
DpnI GATC 3 cut(s) 550, 734, 891
DpnII GATC 3 cut(s) 548, 732, 889
DraIII CACNNNGTG 1 cut(s) 248
EaeI YGGCCR 3 cut(s) 33, 195, 502
Eam1104I CTCTTC 1 cut(s) 372
EarI CTCTTC 1 cut(s) 372
Eco130I CCWWGG 1 cut(s) 499
Eco31I GGTCTC 1 cut(s) 92
Eco47I GGWCC 1 cut(s) 397
Eco57I CTGAAG 2 cut(s) 164, 396
Eco88I CYCGRG 2 cut(s) 7, 43
EcoT14I CCWWGG 1 cut(s) 499
ErhI CCWWGG 1 cut(s) 499
Esp3I CGTCTC 1 cut(s) 46
FaeI CATG 4 cut(s) 503, 639, 697, 942
FaqI GGGAC 1 cut(s) 871
FatI CATG 4 cut(s) 499, 635, 693, 938
FauI CCCGC 1 cut(s) 503
FblI GTMKAC 1 cut(s) 443
Fnu4HI GCNGC 2 cut(s) 117, 814
FokI GGATG 3 cut(s) 77, 989, 995
Fsp4HI GCNGC 2 cut(s) 117, 814
FspBI CTAG 1 cut(s) 245
GlaI GCGC 2 cut(s) 49, 282
GluI GCNGC 2 cut(s) 117, 814
GsuI CTGGAG 2 cut(s) 46, 290
HaeII RGCGCY 1 cut(s) 51
HaeIII GGCC 5 cut(s) 35, 98, 111, 197, 504
HapII CCGG 1 cut(s) 94
HgaI GACGC 1 cut(s) 91
HhaI GCGC 2 cut(s) 50, 283
Hin1II CATG 4 cut(s) 503, 639, 697, 942
Hin6I GCGC 2 cut(s) 48, 281
HinP1I GCGC 2 cut(s) 48, 281
HincII GTYRAC 1 cut(s) 106
HindII GTYRAC 1 cut(s) 106
HinfI GANTC 2 cut(s) 149, 531
HpaII CCGG 1 cut(s) 94
HphI GGTGA 1 cut(s) 363
Hpy166II GTNNAC 5 cut(s) 106, 217, 444, 750, 915
Hpy188I TCNGA 4 cut(s) 144, 732, 826, 930
Hpy188III TCNNGA 3 cut(s) 43, 76, 307
Hpy8I GTNNAC 5 cut(s) 106, 217, 444, 750, 915
Hpy99I CGWCG 1 cut(s) 41
HpyAV CCTTC 5 cut(s) 13, 61, 76, 139, 967
HpyCH4III ACNGT 5 cut(s) 488, 559, 847, 862, 1009
HpyCH4IV ACGT 1 cut(s) 213
HpyCH4V TGCA 6 cut(s) 251, 431, 587, 656, 776, 871
HpyF10VI GCNNNNNNNGC 2 cut(s) 108, 278
HpyF3I CTNAG 2 cut(s) 354, 389
HpySE526I ACGT 1 cut(s) 213
Hsp92II CATG 4 cut(s) 503, 639, 697, 942
HspAI GCGC 2 cut(s) 48, 281
Kzo9I GATC 3 cut(s) 548, 732, 889
LguI GCTCTTC 1 cut(s) 372
LmnI GCTCC 2 cut(s) 65, 496
Lsp1109I GCAGC 2 cut(s) 103, 825
LweI GCATC 1 cut(s) 830
MaeI CTAG 1 cut(s) 245
MaeII ACGT 1 cut(s) 213
MaeIII GTNAC 4 cut(s) 172, 235, 463, 658
MalI GATC 3 cut(s) 550, 734, 891
MboI GATC 3 cut(s) 548, 732, 889
MboII GAAGA 3 cut(s) 91, 389, 1004
MfeI CAATTG 2 cut(s) 576, 720
MflI RGATCY 1 cut(s) 889
MhlI GDGCHC 1 cut(s) 493
MlsI TGGCCA 2 cut(s) 197, 504
MluCI AATT 9 cut(s) 13, 199, 413, 576, 677, 682, 720, 900, 997
MluNI TGGCCA 2 cut(s) 197, 504
MlyI GAGTC 1 cut(s) 158
MmeI TCCRAC 1 cut(s) 681
MnlI CCTC 5 cut(s) 122, 460, 598, 973, 1013
Mox20I TGGCCA 2 cut(s) 197, 504
MroXI GAANNNNTTC 1 cut(s) 23
MscI TGGCCA 2 cut(s) 197, 504
MseI TTAA 5 cut(s) 293, 554, 680, 786, 899
MslI CAYNNNNRTG 1 cut(s) 845
Msp20I TGGCCA 2 cut(s) 197, 504
MspI CCGG 1 cut(s) 94
MspR9I CCNGG 1 cut(s) 95
MunI CAATTG 2 cut(s) 576, 720
MwoI GCNNNNNNNGC 2 cut(s) 108, 278
NciI CCSGG 1 cut(s) 95
NcoI CCATGG 1 cut(s) 499
NdeII GATC 3 cut(s) 548, 732, 889
NlaIII CATG 4 cut(s) 503, 639, 697, 942
NlaIV GGNNCC 1 cut(s) 61
NmuCI GTSAC 1 cut(s) 463
PaeR7I CTCGAG 2 cut(s) 7, 43
PaqCI CACCTGC 1 cut(s) 232
PciSI GCTCTTC 1 cut(s) 372
PdmI GAANNNNTTC 1 cut(s) 23
PfeI GAWTC 1 cut(s) 531
PflMI CCANNNNNTGG 1 cut(s) 313
PkrI GCNGC 2 cut(s) 118, 815
PleI GAGTC 1 cut(s) 157
PpsI GAGTC 1 cut(s) 157
PspN4I GGNNCC 1 cut(s) 61
PspPI GGNCC 3 cut(s) 96, 110, 397
PspXI VCTCGAGB 1 cut(s) 7
PsrI GAACNNNNNNTAC 2 cut(s) 331, 363
PsuI RGATCY 1 cut(s) 889
RsaI GTAC 8 cut(s) 5, 363, 474, 485, 610, 877, 914, 944
RsaNI GTAC 8 cut(s) 4, 362, 473, 484, 609, 876, 913, 943
RseI CAYNNNNRTG 1 cut(s) 845
SapI GCTCTTC 1 cut(s) 372
SaqAI TTAA 5 cut(s) 293, 554, 680, 786, 899
SatI GCNGC 2 cut(s) 117, 814
Sau3AI GATC 3 cut(s) 548, 732, 889
Sau96I GGNCC 3 cut(s) 96, 110, 397
ScaI AGTACT 1 cut(s) 474
SchI GAGTC 1 cut(s) 158
ScrFI CCNGG 1 cut(s) 95
SduI GDGCHC 1 cut(s) 493
SfaNI GCATC 1 cut(s) 830
Sfr274I CTCGAG 2 cut(s) 7, 43
SinI GGWCC 1 cut(s) 397
SlaI CTCGAG 2 cut(s) 7, 43
SmiMI CAYNNNNRTG 1 cut(s) 845
SmlI CTYRAG 2 cut(s) 7, 43
SmoI CTYRAG 2 cut(s) 7, 43
Sse9I AATT 9 cut(s) 13, 199, 413, 576, 677, 682, 720, 900, 997
SsiI CCGC 1 cut(s) 496
SspMI CTAG 1 cut(s) 245
StyD4I CCNGG 1 cut(s) 93
StyI CCWWGG 1 cut(s) 499
TaaI ACNGT 5 cut(s) 488, 559, 847, 862, 1009
TaiI ACGT 1 cut(s) 216
TaqI TCGA 2 cut(s) 8, 44
TaqII GACCGA 1 cut(s) 385
TasI AATT 9 cut(s) 13, 199, 413, 576, 677, 682, 720, 900, 997
TatI WGTACW 6 cut(s) 3, 361, 472, 483, 875, 912
TfiI GAWTC 1 cut(s) 531
Tru1I TTAA 5 cut(s) 293, 554, 680, 786, 899
Tru9I TTAA 5 cut(s) 293, 554, 680, 786, 899
TscAI CASTG 3 cut(s) 127, 778, 850
TseFI GTSAC 1 cut(s) 463
TseI GCWGC 2 cut(s) 116, 813
Tsp45I GTSAC 1 cut(s) 463
TspDTI ATGAA 4 cut(s) 16, 43, 58, 829
TspRI CASTG 3 cut(s) 127, 778, 850
Van91I CCANNNNNTGG 1 cut(s) 313
VpaK11BI GGWCC 1 cut(s) 397
XhoI CTCGAG 2 cut(s) 7, 43
XmiI GTMKAC 1 cut(s) 443
XmnI GAANNNNTTC 1 cut(s) 23
XspI CTAG 1 cut(s) 245
ZrmI AGTACT 1 cut(s) 474
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.