Rw2G024800

Acts as a Mg(2 ) transporter. Can also transport other divalent cations such as Fe(2 ), Sr(2 ), Ba(2 ), Mn(2 ) and Co(2 ) but to a much less extent than Mg(2 )

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000984
Physical Location & Seq
Forward (+)
36865330 .. 36870560
5231 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G024800.1

Sequence Viewer

Length: 1035 bp
ATGTCATCGAGCAATTTGAAAGGGTTCATATTGGCCGTGGTCTCAAGCGCCTTCATTGGCTCCAGCTTCATCATCAAGAAGAAGGGTCTCCGCAGAGCCGGCAACAATGGAACTCGTGCCAGTTCTGGAGGATATGGGTATCTGCGGGAGCCTCTATGGTGGATTGGAATGGTCACAATGGTTGTTGGAGAGATAGCAAATTTTGTAGCATACATATACGCACCCGCCGGGCTTGTCACGCCGCTTGGTGCTTTGAGTATCATTGTTAGTGCGGTGTTAGCGCATTTCCTGTTAAAGGAGAAACTCCAGAAAATGGGAATGTTGGGCTGCCTTTTATGCATAGTGGGTTCGGTTGTGATTGTACTCCATGCACCCGAAGAGCAGACTCTTACTTCCGTCCAACAAATCTGGGATTTAGCAATACAACCAGCTTTCCTTTTGTATACAGCCTCAGTGATAGCTGTAACGCTAGTCCTTATCTTGTATTGTTCCCCCCGCTATGGCCAAACCAATATACTGATTTATATAGGAATATGCTCAATTATCGGATCATTAACTGTCATGAGCATAAAAGCCATTGGCATTGCAATAAAACTCACAATAGAGGGCACAAACCAGGCAGTATACTTCCAAACATGGATTTTTGCAATGGTTGCAGTTACATGCATCATCACTCAATTAAATTACCTAAATATGGCATTGGATACTTTCAACACAGCAGTCGTTTCTCCCATCTATTATGCTATGTTCACATCTTTCACAATACTTGCGAGTGCAATAATGTTTAAGGATTATTCTGGTCAAAGTGCAAGCAGCATAGCTTCAGAATTTTGTGGATTCATCACTGTGTTATCTGGGACTGCTATATTGCATAGTACAAGAGAACCAGATCCACCTTTACTTACAGATTTGTATACGCCATTATCTCCTAAAGTATCATGGTACATCCAAAGCAATGGAGAATGGAAGCAGAAGGATGAAGACGGTTCAGCCCCCAATTTCATCACAGTACTTCGGCCAGACTATTTCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mg_trans_NIPA

Protein Analysis

344

Amino Acids

37.42

Weight (kDa)

8.43

Isoelectric Point (pI)

31.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mg_trans_NIPA PF05653 5 - 295 7.6e-132 Magnesium transporter NIPA
EamA PF00892 55 - 121 2.1e-07 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 313
AccI GTMKAC 3 cut(s) 443, 624, 914
AciI CCGC 6 cut(s) 91, 145, 225, 242, 272, 496
AclWI GGATC 2 cut(s) 556, 884
AcoI YGGCCR 3 cut(s) 33, 502, 1016
AcsI RAATTY 2 cut(s) 199, 827
AcuI CTGAAG 1 cut(s) 807
AfaI GTAC 4 cut(s) 363, 877, 944, 1011
AfiI CCNNNNNNNGG 4 cut(s) 295, 313, 500, 694
AgsI TTSAA 3 cut(s) 19, 712, 1030
AjnI CCWGG 1 cut(s) 615
AluBI AGCT 4 cut(s) 66, 431, 461, 821
AluI AGCT 4 cut(s) 66, 431, 461, 821
Alw26I GTCTC 2 cut(s) 46, 92
AlwI GGATC 2 cut(s) 556, 884
AoxI GGCC 3 cut(s) 33, 502, 1016
ApeKI GCWGC 2 cut(s) 327, 813
ApoI RAATTY 2 cut(s) 199, 827
Asp700I GAANNNNTTC 1 cut(s) 23
AspLEI GCGC 2 cut(s) 50, 283
AsuC2I CCSGG 1 cut(s) 229
BaeGI GKGCMC 1 cut(s) 611
BalI TGGCCA 1 cut(s) 504
BauI CACGAG 1 cut(s) 114
BbsI GAAGAC 1 cut(s) 987
BbvI GCAGC 2 cut(s) 314, 825
BccI CCATC 1 cut(s) 740
BceAI ACGGC 1 cut(s) 20
BciT130I CCWGG 1 cut(s) 617
BciVI GTATCC 1 cut(s) 697
BcnI CCSGG 1 cut(s) 229
BcoDI GTCTC 2 cut(s) 46, 92
BfaI CTAG 1 cut(s) 470
BfoI RGCGCY 1 cut(s) 51
BfuI GTATCC 1 cut(s) 697
BisI GCNGC 3 cut(s) 242, 328, 814
BlsI GCNGC 3 cut(s) 243, 329, 815
BmcAI AGTACT 1 cut(s) 1011
Bme1390I CCNGG 2 cut(s) 229, 617
BmiI GGNNCC 2 cut(s) 61, 150
BmrFI CCNGG 2 cut(s) 229, 617
BmsI GCATC 1 cut(s) 675
BpiI GAAGAC 1 cut(s) 987
BpmI CTGGAG 3 cut(s) 46, 147, 290
BpuEI CTTGAG 1 cut(s) 28
BpuMI CCSGG 1 cut(s) 229
BsaI GGTCTC 2 cut(s) 46, 92
BsaJI CCNNGG 1 cut(s) 36
Bsc4I CCNNNNNNNGG 4 cut(s) 295, 313, 500, 694
Bse118I RCCGGY 1 cut(s) 98
Bse1I ACTGG 1 cut(s) 120
Bse3DI GCAATG 3 cut(s) 582, 654, 961
BseBI CCWGG 1 cut(s) 617
BseDI CCNNGG 1 cut(s) 36
BseGI GGATG 2 cut(s) 945, 982
BseLI CCNNNNNNNGG 4 cut(s) 295, 313, 500, 694
BseMI GCAATG 3 cut(s) 582, 654, 961
BseMII CTCAG 1 cut(s) 465
BseNI ACTGG 1 cut(s) 120
BseSI GKGCMC 1 cut(s) 611
BseXI GCAGC 2 cut(s) 314, 825
BshFI GGCC 3 cut(s) 35, 504, 1018
BsiSI CCGG 2 cut(s) 99, 228
BslFI GGGAC 1 cut(s) 871
BslI CCNNNNNNNGG 4 cut(s) 295, 313, 500, 694
BsmAI GTCTC 2 cut(s) 46, 92
BsmFI GGGAC 1 cut(s) 871
BsnI GGCC 3 cut(s) 35, 504, 1018
Bso31I GGTCTC 2 cut(s) 46, 92
Bsp1286I GDGCHC 1 cut(s) 611
Bsp143I GATC 2 cut(s) 548, 889
BspACI CCGC 6 cut(s) 91, 145, 225, 242, 272, 496
BspANI GGCC 3 cut(s) 35, 504, 1018
BspCNI CTCAG 1 cut(s) 464
BspHI TCATGA 1 cut(s) 561
BspLI GGNNCC 2 cut(s) 61, 150
BspPI GGATC 2 cut(s) 556, 884
BspQI GCTCTTC 1 cut(s) 372
BspTNI GGTCTC 2 cut(s) 46, 92
BsrDI GCAATG 3 cut(s) 582, 654, 961
BsrFI RCCGGY 1 cut(s) 98
BsrI ACTGG 1 cut(s) 120
BssAI RCCGGY 1 cut(s) 98
BssECI CCNNGG 1 cut(s) 36
BssMI GATC 2 cut(s) 548, 889
BssNAI GTATAC 3 cut(s) 444, 625, 915
BssSI CACGAG 1 cut(s) 114
Bst1107I GTATAC 3 cut(s) 444, 625, 915
Bst2BI CACGAG 1 cut(s) 114
Bst2UI CCWGG 1 cut(s) 617
Bst4CI ACNGT 4 cut(s) 559, 847, 986, 1009
Bst6I CTCTTC 1 cut(s) 372
BstAPI GCANNNNNTGC 1 cut(s) 653
BstC8I GCNNGC 2 cut(s) 100, 811
BstDEI CTNAG 1 cut(s) 451
BstDSI CCRYGG 1 cut(s) 36
BstENI CCTNNNNNAGG 1 cut(s) 293
BstF5I GGATG 2 cut(s) 945, 982
BstH2I RGCGCY 1 cut(s) 51
BstHHI GCGC 2 cut(s) 50, 283
BstKTI GATC 2 cut(s) 551, 892
BstMAI GTCTC 2 cut(s) 46, 92
BstMBI GATC 2 cut(s) 548, 889
BstMWI GCNNNNNNNGC 5 cut(s) 99, 238, 278, 336, 653
BstNI CCWGG 1 cut(s) 617
BstNSI RCATGY 1 cut(s) 666
BstSCI CCNGG 2 cut(s) 227, 615
BstSLI GKGCMC 1 cut(s) 611
BstV1I GCAGC 2 cut(s) 314, 825
BstV2I GAAGAC 1 cut(s) 987
BstX2I RGATCY 1 cut(s) 889
BstXI CCANNNNNNTGG 1 cut(s) 956
BstYI RGATCY 1 cut(s) 889
BstZ17I GTATAC 3 cut(s) 444, 625, 915
BsuI GTATCC 1 cut(s) 697
BsuRI GGCC 3 cut(s) 35, 504, 1018
BtgI CCRYGG 1 cut(s) 36
BtsCI GGATG 2 cut(s) 945, 982
BtsIMutI CAGTG 2 cut(s) 459, 843
Cac8I GCNNGC 2 cut(s) 100, 811
CciI TCATGA 1 cut(s) 561
CfoI GCGC 2 cut(s) 50, 283
Cfr10I RCCGGY 1 cut(s) 98
Csp6I GTAC 4 cut(s) 362, 876, 943, 1010
CviAII CATG 5 cut(s) 368, 562, 636, 663, 939
CviQI GTAC 4 cut(s) 362, 876, 943, 1010
DdeI CTNAG 1 cut(s) 451
DpnI GATC 2 cut(s) 550, 891
DpnII GATC 2 cut(s) 548, 889
EaeI YGGCCR 3 cut(s) 33, 502, 1016
Eam1104I CTCTTC 1 cut(s) 372
EarI CTCTTC 1 cut(s) 372
Eco31I GGTCTC 2 cut(s) 46, 92
Eco57I CTGAAG 1 cut(s) 807
EcoNI CCTNNNNNAGG 1 cut(s) 293
EcoRII CCWGG 1 cut(s) 615
EcoT22I ATGCAT 2 cut(s) 341, 668
FaeI CATG 5 cut(s) 371, 565, 639, 666, 942
FaqI GGGAC 1 cut(s) 871
FatI CATG 5 cut(s) 367, 561, 635, 662, 938
FauI CCCGC 3 cut(s) 138, 232, 503
FblI GTMKAC 3 cut(s) 443, 624, 914
Fnu4HI GCNGC 3 cut(s) 242, 328, 814
FokI GGATG 2 cut(s) 932, 989
Fsp4HI GCNGC 3 cut(s) 242, 328, 814
FspBI CTAG 1 cut(s) 470
GlaI GCGC 2 cut(s) 49, 282
GluI GCNGC 3 cut(s) 242, 328, 814
GsuI CTGGAG 3 cut(s) 46, 147, 290
HaeII RGCGCY 1 cut(s) 51
HaeIII GGCC 3 cut(s) 35, 504, 1018
HapII CCGG 2 cut(s) 99, 228
HhaI GCGC 2 cut(s) 50, 283
Hin1II CATG 5 cut(s) 371, 565, 639, 666, 942
Hin6I GCGC 2 cut(s) 48, 281
HinP1I GCGC 2 cut(s) 48, 281
HinfI GANTC 2 cut(s) 385, 837
HpaII CCGG 2 cut(s) 99, 228
Hpy166II GTNNAC 4 cut(s) 444, 625, 750, 915
Hpy188I TCNGA 2 cut(s) 548, 826
Hpy188III TCNNGA 4 cut(s) 76, 126, 307, 562
Hpy8I GTNNAC 4 cut(s) 444, 625, 750, 915
HpyAV CCTTC 3 cut(s) 61, 76, 967
HpyCH4III ACNGT 4 cut(s) 559, 847, 986, 1009
HpyCH4V TGCA 9 cut(s) 339, 371, 587, 647, 656, 666, 776, 809, 871
HpyF10VI GCNNNNNNNGC 5 cut(s) 99, 238, 278, 336, 653
HpyF3I CTNAG 1 cut(s) 451
Hsp92II CATG 5 cut(s) 371, 565, 639, 666, 942
HspAI GCGC 2 cut(s) 48, 281
KroI GCCGGC 1 cut(s) 98
KroNI GCCGGC 1 cut(s) 100
Kzo9I GATC 2 cut(s) 548, 889
LguI GCTCTTC 1 cut(s) 372
LmnI GCTCC 2 cut(s) 65, 148
Lsp1109I GCAGC 2 cut(s) 314, 825
LweI GCATC 1 cut(s) 675
MaeI CTAG 1 cut(s) 470
MaeIII GTNAC 4 cut(s) 172, 235, 463, 658
MalI GATC 2 cut(s) 550, 891
MboI GATC 2 cut(s) 548, 889
MboII GAAGA 3 cut(s) 91, 389, 992
MflI RGATCY 1 cut(s) 889
MhlI GDGCHC 1 cut(s) 611
MlsI TGGCCA 1 cut(s) 504
MluCI AATT 7 cut(s) 13, 199, 540, 677, 682, 827, 997
MluNI TGGCCA 1 cut(s) 504
MlyI GAGTC 1 cut(s) 379
MmeI TCCRAC 2 cut(s) 166, 424
MnlI CCTC 4 cut(s) 122, 162, 460, 598
Mox20I TGGCCA 1 cut(s) 504
Mph1103I ATGCAT 2 cut(s) 341, 668
MroNI GCCGGC 1 cut(s) 98
MroXI GAANNNNTTC 1 cut(s) 23
MscI TGGCCA 1 cut(s) 504
MseI TTAA 4 cut(s) 293, 554, 680, 786
MslI CAYNNNNRTG 1 cut(s) 845
Msp20I TGGCCA 1 cut(s) 504
MspI CCGG 2 cut(s) 99, 228
MspR9I CCNGG 2 cut(s) 229, 617
MvaI CCWGG 1 cut(s) 617
MwoI GCNNNNNNNGC 5 cut(s) 99, 238, 278, 336, 653
NaeI GCCGGC 1 cut(s) 100
NciI CCSGG 1 cut(s) 229
NdeII GATC 2 cut(s) 548, 889
NgoMIV GCCGGC 1 cut(s) 98
NlaIII CATG 5 cut(s) 371, 565, 639, 666, 942
NlaIV GGNNCC 2 cut(s) 61, 150
NmuCI GTSAC 2 cut(s) 172, 235
NsiI ATGCAT 2 cut(s) 341, 668
NspI RCATGY 1 cut(s) 666
PagI TCATGA 1 cut(s) 561
PciSI GCTCTTC 1 cut(s) 372
PdiI GCCGGC 1 cut(s) 100
PdmI GAANNNNTTC 1 cut(s) 23
PfeI GAWTC 1 cut(s) 837
PflMI CCANNNNNTGG 1 cut(s) 313
PkrI GCNGC 3 cut(s) 243, 329, 815
PleI GAGTC 1 cut(s) 379
PpsI GAGTC 1 cut(s) 379
Psp6I CCWGG 1 cut(s) 615
PspGI CCWGG 1 cut(s) 615
PspN4I GGNNCC 2 cut(s) 61, 150
PsuI RGATCY 1 cut(s) 889
RsaI GTAC 4 cut(s) 363, 877, 944, 1011
RsaNI GTAC 4 cut(s) 362, 876, 943, 1010
RseI CAYNNNNRTG 1 cut(s) 845
SapI GCTCTTC 1 cut(s) 372
SaqAI TTAA 4 cut(s) 293, 554, 680, 786
SatI GCNGC 3 cut(s) 242, 328, 814
Sau3AI GATC 2 cut(s) 548, 889
ScaI AGTACT 1 cut(s) 1011
SchI GAGTC 1 cut(s) 379
ScrFI CCNGG 2 cut(s) 229, 617
SduI GDGCHC 1 cut(s) 611
SetI ASST 6 cut(s) 68, 433, 463, 690, 823, 898
SfaNI GCATC 1 cut(s) 675
SmiMI CAYNNNNRTG 1 cut(s) 845
SmlI CTYRAG 1 cut(s) 43
SmoI CTYRAG 1 cut(s) 43
Sse9I AATT 7 cut(s) 13, 199, 540, 677, 682, 827, 997
SsiI CCGC 6 cut(s) 91, 145, 225, 242, 272, 496
SspMI CTAG 1 cut(s) 470
StyD4I CCNGG 2 cut(s) 227, 615
TaaI ACNGT 4 cut(s) 559, 847, 986, 1009
TaqI TCGA 1 cut(s) 8
TasI AATT 7 cut(s) 13, 199, 540, 677, 682, 827, 997
TatI WGTACW 3 cut(s) 361, 875, 1009
TauI GCSGC 1 cut(s) 244
TfiI GAWTC 1 cut(s) 837
Tru1I TTAA 4 cut(s) 293, 554, 680, 786
Tru9I TTAA 4 cut(s) 293, 554, 680, 786
TscAI CASTG 2 cut(s) 459, 850
TseFI GTSAC 2 cut(s) 172, 235
TseI GCWGC 2 cut(s) 327, 813
Tsp45I GTSAC 2 cut(s) 172, 235
TspDTI ATGAA 6 cut(s) 16, 43, 58, 829, 991, 993
TspGWI ACGGA 1 cut(s) 385
TspRI CASTG 2 cut(s) 459, 850
Van91I CCANNNNNTGG 1 cut(s) 313
XagI CCTNNNNNAGG 1 cut(s) 293
XapI RAATTY 2 cut(s) 199, 827
XceI RCATGY 1 cut(s) 666
XmiI GTMKAC 3 cut(s) 443, 624, 914
XmnI GAANNNNTTC 1 cut(s) 23
XspI CTAG 1 cut(s) 470
ZrmI AGTACT 1 cut(s) 1011
Zsp2I ATGCAT 2 cut(s) 341, 668
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.