Acts as a Mg(2 ) transporter. Can also transport other divalent cations such as Fe(2 ), Sr(2 ), Ba(2 ), Mn(2 ) and Co(2 ) but to a much less extent than Mg(2 )
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 907 |
| Acc36I | ACCTGC | 2 cut(s) | 347, 907 |
| AccII | CGCG | 1 cut(s) | 99 |
| AciI | CCGC | 3 cut(s) | 102, 105, 108 |
| AclI | AACGTT | 1 cut(s) | 970 |
| AclWI | GGATC | 2 cut(s) | 358, 361 |
| AcsI | RAATTY | 2 cut(s) | 394, 988 |
| AcuI | CTGAAG | 2 cut(s) | 98, 981 |
| AcyI | GRCGYC | 1 cut(s) | 44 |
| AdeI | CACNNNGTG | 1 cut(s) | 251 |
| AfaI | GTAC | 1 cut(s) | 932 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 60, 503, 697 |
| AgsI | TTSAA | 4 cut(s) | 298, 494, 715, 790 |
| AjnI | CCWGG | 1 cut(s) | 417 |
| AleI | CACNNNNGTG | 1 cut(s) | 160 |
| AluBI | AGCT | 3 cut(s) | 69, 598, 626 |
| AluI | AGCT | 3 cut(s) | 69, 598, 626 |
| Alw21I | GWGCWC | 1 cut(s) | 256 |
| Alw26I | GTCTC | 1 cut(s) | 95 |
| Alw44I | GTGCAC | 1 cut(s) | 252 |
| AlwI | GGATC | 2 cut(s) | 358, 361 |
| Ama87I | CYCGRG | 1 cut(s) | 117 |
| ApaLI | GTGCAC | 1 cut(s) | 252 |
| ApeKI | GCWGC | 2 cut(s) | 719, 816 |
| ApoI | RAATTY | 2 cut(s) | 394, 988 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 538 |
| AspLEI | GCGC | 3 cut(s) | 53, 99, 101 |
| AspS9I | GGNCC | 1 cut(s) | 415 |
| AsuHPI | GGTGA | 1 cut(s) | 169 |
| AvaI | CYCGRG | 1 cut(s) | 117 |
| AvaII | GGWCC | 1 cut(s) | 415 |
| AxyI | CCTNAGG | 1 cut(s) | 378 |
| BaeGI | GKGCMC | 1 cut(s) | 256 |
| Bbv12I | GWGCWC | 1 cut(s) | 256 |
| BbvI | GCAGC | 2 cut(s) | 706, 828 |
| BccI | CCATC | 3 cut(s) | 34, 541, 872 |
| BciT130I | CCWGG | 1 cut(s) | 419 |
| BciVI | GTATCC | 1 cut(s) | 700 |
| BclI | TGATCA | 1 cut(s) | 174 |
| BcoDI | GTCTC | 1 cut(s) | 95 |
| BfaI | CTAG | 3 cut(s) | 149, 467, 774 |
| BfmI | CTRYAG | 1 cut(s) | 399 |
| BfoI | RGCGCY | 1 cut(s) | 54 |
| BfuAI | ACCTGC | 2 cut(s) | 347, 907 |
| BfuI | GTATCC | 1 cut(s) | 700 |
| BisI | GCNGC | 5 cut(s) | 102, 105, 108, 720, 817 |
| BlsI | GCNGC | 5 cut(s) | 103, 106, 109, 721, 818 |
| Bme1390I | CCNGG | 1 cut(s) | 419 |
| Bme18I | GGWCC | 1 cut(s) | 415 |
| BmeT110I | CYCGRG | 1 cut(s) | 117 |
| BmgT120I | GGNCC | 1 cut(s) | 415 |
| BmiI | GGNNCC | 5 cut(s) | 115, 303, 416, 862, 894 |
| BmrFI | CCNGG | 1 cut(s) | 419 |
| BmrI | ACTGGG | 1 cut(s) | 728 |
| BmsI | GCATC | 1 cut(s) | 351 |
| BmuI | ACTGGG | 1 cut(s) | 728 |
| BpuEI | CTTGAG | 2 cut(s) | 77, 626 |
| BsaBI | GATNNNNATC | 1 cut(s) | 365 |
| BsaHI | GRCGYC | 1 cut(s) | 44 |
| BsaI | GGTCTC | 1 cut(s) | 95 |
| BsaJI | CCNNGG | 1 cut(s) | 497 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 933, 963 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 60, 503, 697 |
| Bse1I | ACTGG | 3 cut(s) | 39, 525, 734 |
| Bse21I | CCTNAGG | 1 cut(s) | 378 |
| Bse8I | GATNNNNATC | 1 cut(s) | 365 |
| BseBI | CCWGG | 1 cut(s) | 419 |
| BseDI | CCNNGG | 1 cut(s) | 497 |
| BseGI | GGATG | 2 cut(s) | 72, 335 |
| BseJI | GATNNNNATC | 1 cut(s) | 365 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 60, 503, 697 |
| BseMII | CTCAG | 2 cut(s) | 248, 392 |
| BseNI | ACTGG | 3 cut(s) | 39, 525, 734 |
| BsePI | GCGCGC | 1 cut(s) | 97 |
| BseRI | GAGGAG | 1 cut(s) | 954 |
| BseSI | GKGCMC | 1 cut(s) | 256 |
| BseXI | GCAGC | 2 cut(s) | 706, 828 |
| Bsh1236I | CGCG | 1 cut(s) | 99 |
| BsiHKAI | GWGCWC | 1 cut(s) | 256 |
| BsiHKCI | CYCGRG | 1 cut(s) | 117 |
| BsiSI | CCGG | 2 cut(s) | 111, 227 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 60, 503, 697 |
| BsmAI | GTCTC | 1 cut(s) | 95 |
| Bso31I | GGTCTC | 1 cut(s) | 95 |
| BsoBI | CYCGRG | 1 cut(s) | 117 |
| Bsp1286I | GDGCHC | 1 cut(s) | 256 |
| Bsp143I | GATC | 4 cut(s) | 174, 350, 366, 886 |
| BspACI | CCGC | 3 cut(s) | 102, 105, 108 |
| BspCNI | CTCAG | 2 cut(s) | 249, 391 |
| BspFNI | CGCG | 1 cut(s) | 99 |
| BspLI | GGNNCC | 5 cut(s) | 115, 303, 416, 862, 894 |
| BspMI | ACCTGC | 2 cut(s) | 347, 907 |
| BspPI | GGATC | 2 cut(s) | 358, 361 |
| BspTNI | GGTCTC | 1 cut(s) | 95 |
| BsrI | ACTGG | 3 cut(s) | 39, 525, 734 |
| BssECI | CCNNGG | 1 cut(s) | 497 |
| BssHII | GCGCGC | 1 cut(s) | 97 |
| BssMI | GATC | 4 cut(s) | 174, 350, 366, 886 |
| BssNI | GRCGYC | 1 cut(s) | 44 |
| Bst2UI | CCWGG | 1 cut(s) | 419 |
| Bst4CI | ACNGT | 5 cut(s) | 562, 655, 681, 850, 1008 |
| Bst6I | CTCTTC | 1 cut(s) | 947 |
| BstACI | GRCGYC | 1 cut(s) | 44 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 728 |
| BstC8I | GCNNGC | 3 cut(s) | 67, 99, 169 |
| BstDEI | CTNAG | 2 cut(s) | 257, 378 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 695 |
| BstF5I | GGATG | 2 cut(s) | 72, 335 |
| BstFNI | CGCG | 1 cut(s) | 99 |
| BstH2I | RGCGCY | 1 cut(s) | 54 |
| BstHHI | GCGC | 3 cut(s) | 53, 99, 101 |
| BstKTI | GATC | 4 cut(s) | 177, 353, 369, 889 |
| BstMAI | GTCTC | 1 cut(s) | 95 |
| BstMBI | GATC | 4 cut(s) | 174, 350, 366, 886 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 50, 107, 281, 728 |
| BstNI | CCWGG | 1 cut(s) | 419 |
| BstSCI | CCNGG | 1 cut(s) | 417 |
| BstSFI | CTRYAG | 1 cut(s) | 399 |
| BstSLI | GKGCMC | 1 cut(s) | 256 |
| BstUI | CGCG | 1 cut(s) | 99 |
| BstV1I | GCAGC | 2 cut(s) | 706, 828 |
| BstXI | CCANNNNNNTGG | 3 cut(s) | 162, 208, 639 |
| Bsu36I | CCTNAGG | 1 cut(s) | 378 |
| BsuI | GTATCC | 1 cut(s) | 700 |
| BtgZI | GCGATG | 1 cut(s) | 53 |
| BtsCI | GGATG | 2 cut(s) | 72, 335 |
| BtsIMutI | CAGTG | 4 cut(s) | 254, 277, 846, 954 |
| BveI | ACCTGC | 2 cut(s) | 347, 907 |
| Cac8I | GCNNGC | 3 cut(s) | 67, 99, 169 |
| CfoI | GCGC | 3 cut(s) | 53, 99, 101 |
| Cfr13I | GGNCC | 1 cut(s) | 415 |
| CseI | GACGC | 1 cut(s) | 33 |
| Csp6I | GTAC | 1 cut(s) | 931 |
| CviAII | CATG | 6 cut(s) | 172, 181, 371, 639, 875, 927 |
| CviJI | RGCY | 9 cut(s) | 69, 167, 212, 284, 434, 598, 626, 701, 824 |
| CviKI_1 | RGCY | 9 cut(s) | 69, 167, 212, 284, 434, 598, 626, 701, 824 |
| CviQI | GTAC | 1 cut(s) | 931 |
| DdeI | CTNAG | 2 cut(s) | 257, 378 |
| DpnI | GATC | 4 cut(s) | 176, 352, 368, 888 |
| DpnII | GATC | 4 cut(s) | 174, 350, 366, 886 |
| DraIII | CACNNNGTG | 1 cut(s) | 251 |
| Eam1104I | CTCTTC | 1 cut(s) | 947 |
| EarI | CTCTTC | 1 cut(s) | 947 |
| Eco31I | GGTCTC | 1 cut(s) | 95 |
| Eco47I | GGWCC | 1 cut(s) | 415 |
| Eco57I | CTGAAG | 2 cut(s) | 98, 981 |
| Eco81I | CCTNAGG | 1 cut(s) | 378 |
| Eco88I | CYCGRG | 1 cut(s) | 117 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 695 |
| EcoO109I | RGGNCCY | 1 cut(s) | 415 |
| EcoRI | GAATTC | 1 cut(s) | 988 |
| EcoRII | CCWGG | 1 cut(s) | 417 |
| EcoT22I | ATGCAT | 1 cut(s) | 142 |
| FaeI | CATG | 6 cut(s) | 175, 184, 374, 642, 878, 930 |
| FatI | CATG | 6 cut(s) | 171, 180, 370, 638, 874, 926 |
| FbaI | TGATCA | 1 cut(s) | 174 |
| Fnu4HI | GCNGC | 5 cut(s) | 102, 105, 108, 720, 817 |
| FokI | GGATG | 2 cut(s) | 59, 342 |
| Fsp4HI | GCNGC | 5 cut(s) | 102, 105, 108, 720, 817 |
| FspBI | CTAG | 3 cut(s) | 149, 467, 774 |
| GlaI | GCGC | 3 cut(s) | 52, 98, 100 |
| GluI | GCNGC | 5 cut(s) | 102, 105, 108, 720, 817 |
| HaeII | RGCGCY | 1 cut(s) | 54 |
| HapII | CCGG | 2 cut(s) | 111, 227 |
| HgaI | GACGC | 1 cut(s) | 33 |
| HhaI | GCGC | 3 cut(s) | 53, 99, 101 |
| Hin1I | GRCGYC | 1 cut(s) | 44 |
| Hin1II | CATG | 6 cut(s) | 175, 184, 374, 642, 878, 930 |
| Hin6I | GCGC | 3 cut(s) | 51, 97, 99 |
| HinP1I | GCGC | 3 cut(s) | 51, 97, 99 |
| HinfI | GANTC | 3 cut(s) | 534, 840, 944 |
| HpaII | CCGG | 2 cut(s) | 111, 227 |
| HphI | GGTGA | 1 cut(s) | 169 |
| Hpy166II | GTNNAC | 3 cut(s) | 254, 554, 753 |
| Hpy188I | TCNGA | 8 cut(s) | 13, 621, 651, 762, 807, 829, 961, 994 |
| Hpy188III | TCNNGA | 5 cut(s) | 76, 380, 605, 858, 890 |
| Hpy8I | GTNNAC | 3 cut(s) | 254, 554, 753 |
| Hpy99I | CGWCG | 1 cut(s) | 49 |
| HpyAV | CCTTC | 3 cut(s) | 64, 79, 292 |
| HpyCH4III | ACNGT | 5 cut(s) | 562, 655, 681, 850, 1008 |
| HpyCH4IV | ACGT | 2 cut(s) | 710, 970 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 50, 107, 281, 728 |
| HpyF3I | CTNAG | 2 cut(s) | 257, 378 |
| HpySE526I | ACGT | 2 cut(s) | 710, 970 |
| Hsp92I | GRCGYC | 1 cut(s) | 44 |
| Hsp92II | CATG | 6 cut(s) | 175, 184, 374, 642, 878, 930 |
| HspAI | GCGC | 3 cut(s) | 51, 97, 99 |
| Ksp22I | TGATCA | 1 cut(s) | 174 |
| Kzo9I | GATC | 4 cut(s) | 174, 350, 366, 886 |
| LmnI | GCTCC | 3 cut(s) | 229, 382, 907 |
| Lsp1109I | GCAGC | 2 cut(s) | 706, 828 |
| LweI | GCATC | 1 cut(s) | 351 |
| MaeI | CTAG | 3 cut(s) | 149, 467, 774 |
| MaeII | ACGT | 2 cut(s) | 710, 970 |
| MaeIII | GTNAC | 5 cut(s) | 238, 334, 454, 673, 922 |
| MalI | GATC | 4 cut(s) | 176, 352, 368, 888 |
| MboI | GATC | 4 cut(s) | 174, 350, 366, 886 |
| MboII | GAAGA | 4 cut(s) | 91, 94, 964, 974 |
| MfeI | CAATTG | 2 cut(s) | 579, 723 |
| MhlI | GDGCHC | 1 cut(s) | 256 |
| MlyI | GAGTC | 1 cut(s) | 953 |
| MmeI | TCCRAC | 1 cut(s) | 307 |
| MnlI | CCTC | 6 cut(s) | 387, 397, 507, 601, 835, 932 |
| Mph1103I | ATGCAT | 1 cut(s) | 142 |
| MroXI | GAANNNNTTC | 1 cut(s) | 538 |
| MseI | TTAA | 2 cut(s) | 683, 693 |
| MslI | CAYNNNNRTG | 3 cut(s) | 160, 637, 848 |
| MspI | CCGG | 2 cut(s) | 111, 227 |
| MspR9I | CCNGG | 1 cut(s) | 419 |
| MunI | CAATTG | 2 cut(s) | 579, 723 |
| MvaI | CCWGG | 1 cut(s) | 419 |
| MvnI | CGCG | 1 cut(s) | 99 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 50, 107, 281, 728 |
| NdeII | GATC | 4 cut(s) | 174, 350, 366, 886 |
| NlaIII | CATG | 6 cut(s) | 175, 184, 374, 642, 878, 930 |
| NlaIV | GGNNCC | 5 cut(s) | 115, 303, 416, 862, 894 |
| NsiI | ATGCAT | 1 cut(s) | 142 |
| OliI | CACNNNNGTG | 1 cut(s) | 160 |
| PaqCI | CACCTGC | 1 cut(s) | 907 |
| PauI | GCGCGC | 1 cut(s) | 97 |
| PdmI | GAANNNNTTC | 1 cut(s) | 538 |
| PfeI | GAWTC | 2 cut(s) | 534, 840 |
| PkrI | GCNGC | 5 cut(s) | 103, 106, 109, 721, 818 |
| PleI | GAGTC | 1 cut(s) | 952 |
| PpsI | GAGTC | 1 cut(s) | 952 |
| PpuMI | RGGWCCY | 1 cut(s) | 415 |
| Psp1406I | AACGTT | 1 cut(s) | 970 |
| Psp5II | RGGWCCY | 1 cut(s) | 415 |
| Psp6I | CCWGG | 1 cut(s) | 417 |
| PspGI | CCWGG | 1 cut(s) | 417 |
| PspN4I | GGNNCC | 5 cut(s) | 115, 303, 416, 862, 894 |
| PspPI | GGNCC | 1 cut(s) | 415 |
| PspPPI | RGGWCCY | 1 cut(s) | 415 |
| PteI | GCGCGC | 1 cut(s) | 97 |
| RsaI | GTAC | 1 cut(s) | 932 |
| RsaNI | GTAC | 1 cut(s) | 931 |
| RseI | CAYNNNNRTG | 3 cut(s) | 160, 637, 848 |
| SaqAI | TTAA | 2 cut(s) | 683, 693 |
| SatI | GCNGC | 5 cut(s) | 102, 105, 108, 720, 817 |
| Sau3AI | GATC | 4 cut(s) | 174, 350, 366, 886 |
| Sau96I | GGNCC | 1 cut(s) | 415 |
| SchI | GAGTC | 1 cut(s) | 953 |
| ScrFI | CCNGG | 1 cut(s) | 419 |
| SduI | GDGCHC | 1 cut(s) | 256 |
| SfaNI | GCATC | 1 cut(s) | 351 |
| SfcI | CTRYAG | 1 cut(s) | 399 |
| SinI | GGWCC | 1 cut(s) | 415 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 160, 637, 848 |
| SmlI | CTYRAG | 2 cut(s) | 92, 605 |
| SmoI | CTYRAG | 2 cut(s) | 92, 605 |
| SsiI | CCGC | 3 cut(s) | 102, 105, 108 |
| SspMI | CTAG | 3 cut(s) | 149, 467, 774 |
| StyD4I | CCNGG | 1 cut(s) | 417 |
| TaaI | ACNGT | 5 cut(s) | 562, 655, 681, 850, 1008 |
| TaiI | ACGT | 2 cut(s) | 713, 973 |
| TaqI | TCGA | 1 cut(s) | 47 |
| TauI | GCSGC | 3 cut(s) | 104, 107, 110 |
| TfiI | GAWTC | 2 cut(s) | 534, 840 |
| Tru1I | TTAA | 2 cut(s) | 683, 693 |
| Tru9I | TTAA | 2 cut(s) | 683, 693 |
| TscAI | CASTG | 4 cut(s) | 261, 277, 853, 954 |
| TseI | GCWGC | 2 cut(s) | 719, 816 |
| TspDTI | ATGAA | 4 cut(s) | 46, 61, 832, 981 |
| TspRI | CASTG | 4 cut(s) | 261, 277, 853, 954 |
| VneI | GTGCAC | 1 cut(s) | 252 |
| VpaK11BI | GGWCC | 1 cut(s) | 415 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 695 |
| XapI | RAATTY | 2 cut(s) | 394, 988 |
| XmnI | GAANNNNTTC | 1 cut(s) | 538 |
| XspI | CTAG | 3 cut(s) | 149, 467, 774 |
| Zsp2I | ATGCAT | 1 cut(s) | 142 |