Acts as a Mg(2 ) transporter. Can also transport other divalent cations such as Fe(2 ), Sr(2 ), Ba(2 ), Mn(2 ) and Co(2 ) but to a much less extent than Mg(2 )
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 1 cut(s) | 509 |
| AciI | CCGC | 1 cut(s) | 105 |
| AclWI | GGATC | 1 cut(s) | 364 |
| AcsI | RAATTY | 3 cut(s) | 208, 893, 1023 |
| AdeI | CACNNNGTG | 1 cut(s) | 509 |
| AfaI | GTAC | 1 cut(s) | 1049 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 509 |
| AflIII | ACRYGT | 1 cut(s) | 671 |
| AgsI | TTSAA | 5 cut(s) | 304, 413, 721, 885, 985 |
| AluBI | AGCT | 8 cut(s) | 41, 72, 110, 128, 440, 728, 906, 924 |
| AluI | AGCT | 8 cut(s) | 41, 72, 110, 128, 440, 728, 906, 924 |
| Alw26I | GTCTC | 2 cut(s) | 635, 969 |
| AlwI | GGATC | 1 cut(s) | 364 |
| AoxI | GGCC | 2 cut(s) | 28, 91 |
| ApeKI | GCWGC | 2 cut(s) | 107, 110 |
| ApoI | RAATTY | 3 cut(s) | 208, 893, 1023 |
| AspA2I | CCTAGG | 1 cut(s) | 586 |
| AspLEI | GCGC | 1 cut(s) | 709 |
| AsuHPI | GGTGA | 2 cut(s) | 475, 1004 |
| AvrII | CCTAGG | 1 cut(s) | 586 |
| BanII | GRGCYC | 1 cut(s) | 461 |
| BbsI | GAAGAC | 1 cut(s) | 1042 |
| BbvI | GCAGC | 2 cut(s) | 97, 119 |
| BccI | CCATC | 2 cut(s) | 547, 878 |
| BciVI | GTATCC | 1 cut(s) | 946 |
| BcoDI | GTCTC | 2 cut(s) | 635, 969 |
| BfaI | CTAG | 5 cut(s) | 38, 527, 587, 780, 1051 |
| BfoI | RGCGCY | 1 cut(s) | 710 |
| BfuI | GTATCC | 1 cut(s) | 946 |
| BisI | GCNGC | 3 cut(s) | 105, 108, 111 |
| BlnI | CCTAGG | 1 cut(s) | 586 |
| BlsI | GCNGC | 3 cut(s) | 106, 109, 112 |
| BmiI | GGNNCC | 2 cut(s) | 159, 868 |
| BmsI | GCATC | 1 cut(s) | 400 |
| BpiI | GAAGAC | 1 cut(s) | 1042 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 890, 922 |
| BpmI | CTGGAG | 1 cut(s) | 216 |
| BsaJI | CCNNGG | 1 cut(s) | 586 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 149, 179 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 509 |
| Bse1I | ACTGG | 3 cut(s) | 323, 541, 771 |
| Bse3DI | GCAATG | 1 cut(s) | 213 |
| BseDI | CCNNGG | 1 cut(s) | 586 |
| BseGI | GGATG | 3 cut(s) | 391, 811, 908 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 509 |
| BseMI | GCAATG | 1 cut(s) | 213 |
| BseMII | CTCAG | 2 cut(s) | 698, 1008 |
| BseNI | ACTGG | 3 cut(s) | 323, 541, 771 |
| BseRI | GAGGAG | 1 cut(s) | 834 |
| BseXI | GCAGC | 2 cut(s) | 97, 119 |
| BseYI | CCCAGC | 1 cut(s) | 172 |
| BsgI | GTGCAG | 1 cut(s) | 936 |
| BshFI | GGCC | 2 cut(s) | 30, 93 |
| BslFI | GGGAC | 1 cut(s) | 721 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 509 |
| BsmAI | GTCTC | 2 cut(s) | 635, 969 |
| BsmFI | GGGAC | 1 cut(s) | 721 |
| BsmI | GAATGC | 1 cut(s) | 880 |
| BsnI | GGCC | 2 cut(s) | 30, 93 |
| Bsp1286I | GDGCHC | 1 cut(s) | 461 |
| Bsp143I | GATC | 2 cut(s) | 356, 825 |
| BspACI | CCGC | 1 cut(s) | 105 |
| BspANI | GGCC | 2 cut(s) | 30, 93 |
| BspCNI | CTCAG | 2 cut(s) | 697, 1009 |
| BspLI | GGNNCC | 2 cut(s) | 159, 868 |
| BspPI | GGATC | 1 cut(s) | 364 |
| BspQI | GCTCTTC | 1 cut(s) | 94 |
| BsrDI | GCAATG | 1 cut(s) | 213 |
| BsrI | ACTGG | 3 cut(s) | 323, 541, 771 |
| BssECI | CCNNGG | 1 cut(s) | 586 |
| BssMI | GATC | 2 cut(s) | 356, 825 |
| BssT1I | CCWWGG | 1 cut(s) | 586 |
| Bst4CI | ACNGT | 1 cut(s) | 992 |
| Bst6I | CTCTTC | 2 cut(s) | 94, 999 |
| BstC8I | GCNNGC | 3 cut(s) | 70, 130, 288 |
| BstDEI | CTNAG | 2 cut(s) | 684, 1017 |
| BstF5I | GGATG | 3 cut(s) | 391, 811, 908 |
| BstH2I | RGCGCY | 1 cut(s) | 710 |
| BstHHI | GCGC | 1 cut(s) | 709 |
| BstKTI | GATC | 2 cut(s) | 359, 828 |
| BstMAI | GTCTC | 2 cut(s) | 635, 969 |
| BstMBI | GATC | 2 cut(s) | 356, 825 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 110, 287, 776 |
| BstNSI | RCATGY | 1 cut(s) | 675 |
| BstV1I | GCAGC | 2 cut(s) | 97, 119 |
| BstV2I | GAAGAC | 1 cut(s) | 1042 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 645 |
| BsuI | GTATCC | 1 cut(s) | 946 |
| BsuRI | GGCC | 2 cut(s) | 30, 93 |
| BtsCI | GGATG | 3 cut(s) | 391, 811, 908 |
| BtsI | GCAGTG | 1 cut(s) | 243 |
| BtsIMutI | CAGTG | 5 cut(s) | 243, 283, 316, 534, 764 |
| Cac8I | GCNNGC | 3 cut(s) | 70, 130, 288 |
| CfoI | GCGC | 1 cut(s) | 709 |
| Csp6I | GTAC | 1 cut(s) | 1048 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 174, 209 |
| CviAII | CATG | 5 cut(s) | 377, 445, 645, 672, 754 |
| CviQI | GTAC | 1 cut(s) | 1048 |
| DdeI | CTNAG | 2 cut(s) | 684, 1017 |
| DpnI | GATC | 2 cut(s) | 358, 827 |
| DpnII | GATC | 2 cut(s) | 356, 825 |
| DraI | TTTAAA | 1 cut(s) | 916 |
| DraIII | CACNNNGTG | 1 cut(s) | 509 |
| Eam1104I | CTCTTC | 2 cut(s) | 94, 999 |
| EarI | CTCTTC | 2 cut(s) | 94, 999 |
| Eco130I | CCWWGG | 1 cut(s) | 586 |
| Eco147I | AGGCCT | 2 cut(s) | 30, 93 |
| Eco24I | GRGCYC | 1 cut(s) | 461 |
| EcoT14I | CCWWGG | 1 cut(s) | 586 |
| EcoT22I | ATGCAT | 1 cut(s) | 226 |
| EcoT38I | GRGCYC | 1 cut(s) | 461 |
| ErhI | CCWWGG | 1 cut(s) | 586 |
| FaeI | CATG | 5 cut(s) | 380, 448, 648, 675, 757 |
| FalI | AAGNNNNNCTT | 3 cut(s) | 38, 18, 50 |
| FaqI | GGGAC | 1 cut(s) | 721 |
| FatI | CATG | 5 cut(s) | 376, 444, 644, 671, 753 |
| FauNDI | CATATG | 1 cut(s) | 220 |
| Fnu4HI | GCNGC | 3 cut(s) | 105, 108, 111 |
| FokI | GGATG | 3 cut(s) | 378, 818, 895 |
| FriOI | GRGCYC | 1 cut(s) | 461 |
| Fsp4HI | GCNGC | 3 cut(s) | 105, 108, 111 |
| FspBI | CTAG | 5 cut(s) | 38, 527, 587, 780, 1051 |
| GlaI | GCGC | 1 cut(s) | 708 |
| GluI | GCNGC | 3 cut(s) | 105, 108, 111 |
| GsaI | CCCAGC | 1 cut(s) | 176 |
| GsuI | CTGGAG | 1 cut(s) | 216 |
| HaeII | RGCGCY | 1 cut(s) | 710 |
| HaeIII | GGCC | 2 cut(s) | 30, 93 |
| HhaI | GCGC | 1 cut(s) | 709 |
| Hin1II | CATG | 5 cut(s) | 380, 448, 648, 675, 757 |
| Hin6I | GCGC | 1 cut(s) | 707 |
| HinP1I | GCGC | 1 cut(s) | 707 |
| HindIII | AAGCTT | 1 cut(s) | 70 |
| HinfI | GANTC | 3 cut(s) | 622, 977, 1007 |
| HphI | GGTGA | 2 cut(s) | 475, 1004 |
| Hpy166II | GTNNAC | 1 cut(s) | 759 |
| Hpy188I | TCNGA | 6 cut(s) | 98, 125, 687, 699, 835, 1018 |
| Hpy188III | TCNNGA | 7 cut(s) | 82, 301, 354, 400, 410, 638, 864 |
| Hpy8I | GTNNAC | 1 cut(s) | 759 |
| HpyAV | CCTTC | 3 cut(s) | 41, 298, 951 |
| HpyCH4III | ACNGT | 1 cut(s) | 992 |
| HpyCH4V | TGCA | 8 cut(s) | 218, 224, 260, 316, 348, 380, 880, 953 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 110, 287, 776 |
| HpyF3I | CTNAG | 2 cut(s) | 684, 1017 |
| Hsp92II | CATG | 5 cut(s) | 380, 448, 648, 675, 757 |
| HspAI | GCGC | 1 cut(s) | 707 |
| Kzo9I | GATC | 2 cut(s) | 356, 825 |
| LguI | GCTCTTC | 1 cut(s) | 94 |
| LmnI | GCTCC | 4 cut(s) | 157, 235, 388, 821 |
| Lsp1109I | GCAGC | 2 cut(s) | 97, 119 |
| LweI | GCATC | 1 cut(s) | 400 |
| MaeI | CTAG | 5 cut(s) | 38, 527, 587, 780, 1051 |
| MaeIII | GTNAC | 2 cut(s) | 244, 664 |
| MalI | GATC | 2 cut(s) | 358, 827 |
| MboI | GATC | 2 cut(s) | 356, 825 |
| MboII | GAAGA | 6 cut(s) | 26, 111, 481, 1013, 1016, 1047 |
| MhlI | GDGCHC | 1 cut(s) | 461 |
| MluCI | AATT | 8 cut(s) | 208, 270, 366, 602, 771, 893, 1023, 1043 |
| MlyI | GAGTC | 2 cut(s) | 971, 1016 |
| MnlI | CCTC | 9 cut(s) | 104, 171, 193, 261, 303, 607, 812, 966, 1012 |
| Mph1103I | ATGCAT | 1 cut(s) | 226 |
| MseI | TTAA | 8 cut(s) | 24, 263, 579, 605, 629, 795, 915, 1031 |
| MslI | CAYNNNNRTG | 2 cut(s) | 686, 958 |
| MspA1I | CMGCKG | 2 cut(s) | 110, 728 |
| Mva1269I | GAATGC | 1 cut(s) | 880 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 110, 287, 776 |
| NdeI | CATATG | 1 cut(s) | 220 |
| NdeII | GATC | 2 cut(s) | 356, 825 |
| NlaIII | CATG | 5 cut(s) | 380, 448, 648, 675, 757 |
| NlaIV | GGNNCC | 2 cut(s) | 159, 868 |
| NsiI | ATGCAT | 1 cut(s) | 226 |
| NspI | RCATGY | 1 cut(s) | 675 |
| PceI | AGGCCT | 2 cut(s) | 30, 93 |
| PciI | ACATGT | 1 cut(s) | 671 |
| PciSI | GCTCTTC | 1 cut(s) | 94 |
| PctI | GAATGC | 1 cut(s) | 880 |
| PfeI | GAWTC | 1 cut(s) | 622 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 509 |
| PkrI | GCNGC | 3 cut(s) | 106, 109, 112 |
| PleI | GAGTC | 2 cut(s) | 971, 1015 |
| PpsI | GAGTC | 2 cut(s) | 971, 1015 |
| PscI | ACATGT | 1 cut(s) | 671 |
| PspFI | CCCAGC | 1 cut(s) | 172 |
| PspN4I | GGNNCC | 2 cut(s) | 159, 868 |
| PvuII | CAGCTG | 2 cut(s) | 110, 728 |
| RsaI | GTAC | 1 cut(s) | 1049 |
| RsaNI | GTAC | 1 cut(s) | 1048 |
| RseI | CAYNNNNRTG | 2 cut(s) | 686, 958 |
| SapI | GCTCTTC | 1 cut(s) | 94 |
| SaqAI | TTAA | 8 cut(s) | 24, 263, 579, 605, 629, 795, 915, 1031 |
| SatI | GCNGC | 3 cut(s) | 105, 108, 111 |
| Sau3AI | GATC | 2 cut(s) | 356, 825 |
| SchI | GAGTC | 2 cut(s) | 971, 1016 |
| SduI | GDGCHC | 1 cut(s) | 461 |
| SfaNI | GCATC | 1 cut(s) | 400 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 686, 958 |
| Sse9I | AATT | 8 cut(s) | 208, 270, 366, 602, 771, 893, 1023, 1043 |
| SseBI | AGGCCT | 2 cut(s) | 30, 93 |
| SsiI | CCGC | 1 cut(s) | 105 |
| SspMI | CTAG | 5 cut(s) | 38, 527, 587, 780, 1051 |
| StuI | AGGCCT | 2 cut(s) | 30, 93 |
| StyI | CCWWGG | 1 cut(s) | 586 |
| TaaI | ACNGT | 1 cut(s) | 992 |
| TaqII | GACCGA | 1 cut(s) | 349 |
| TasI | AATT | 8 cut(s) | 208, 270, 366, 602, 771, 893, 1023, 1043 |
| TatI | WGTACW | 1 cut(s) | 1047 |
| TauI | GCSGC | 1 cut(s) | 107 |
| TfiI | GAWTC | 1 cut(s) | 622 |
| Tru1I | TTAA | 8 cut(s) | 24, 263, 579, 605, 629, 795, 915, 1031 |
| Tru9I | TTAA | 8 cut(s) | 24, 263, 579, 605, 629, 795, 915, 1031 |
| TscAI | CASTG | 5 cut(s) | 243, 283, 323, 541, 771 |
| TseI | GCWGC | 2 cut(s) | 107, 110 |
| TspRI | CASTG | 5 cut(s) | 243, 283, 323, 541, 771 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 509 |
| XapI | RAATTY | 3 cut(s) | 208, 893, 1023 |
| XceI | RCATGY | 1 cut(s) | 675 |
| XmaJI | CCTAGG | 1 cut(s) | 586 |
| XspI | CTAG | 5 cut(s) | 38, 527, 587, 780, 1051 |
| Zsp2I | ATGCAT | 1 cut(s) | 226 |