Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0000984

Physical Location & Seq

Genomic Coordinates Forward (+)

23786596 .. 23790476

Gene Structure 3881 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1116700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1011 bp

ATGGGGGTTTCGGAGAATGCGAAGGGGTTGATACTGGCGATGGCGTCGAGTGCGTTCATCGGCGCGAGCTTCATCCTGAAAAAGAAGGGTCTCAAGCGCGCCGGGGCCGCCGGCACCCGAGCAGGAGTTGGTGGATATACATACTTATTAGAACCACTCTGGTGGGCTGGCATGATCACTATGATTGTTGGGGAAGTTGCCAATTTCGTGGCCTATGTATATGCTCCGGCAGTTCTTGTAACCCCACTTGGTGCACTGAGTATAATTGTCAGTGCTGTTTTGGCTCACTTCTTGTTGAAGGAACGAATAACGAAAATGGGTATCGTGGGATGTGTTACCTGCATCATGGGATCAGTCGTGATCGTGATCCATGCACCTCAGGAACATACTCTAAATTCTGTAGAGGAAATCTGGGTCCTGGCTACCCAAACAGCCTTTCTGGTTTATGTTGTAGCTACAATTTCCCTAGTGTTAGTTTTGGTCTTGCATTTTGAACCACAATATGGGCAAACGAATATACTAGTCTACTTGGGAATCTGTTCCTTGATGGGTTCACTTACGGTTGTAAGCATAAAGGCAATTGGAATTGCAATAAAGCTAACTCTTGAGGGAGTAAGTCAGATTGCTTATCCTCAGACGTGGTTTTTTCTGACAGTTGCTATAATCTGTGTTGTTACACAGTTAAATTATCTTAACAAGGCTTTGGATACATTCAATGCAGCAATTGTTGCCCCGGTATATTATGTGATGTTTACAACTCTGACCATAATTGCTAGCGTAATAATGTTCAAGGATTGGTCTGGTCAGAATGTCAGCAGCATAGCCTCTGAAATATGTGGATTCATTACTGTGCTGTCAGGAACCATCATACTGCATGCAACAAGAGATCAGGAACCATCTGCTCCACAACCACAAGGAACTGTTACATGGTACATTAGAGGAGATTCAGTGAAGAGTTCTGAAGATGAACGTTTGCTCACTTTACAGAATTCAGATTATCCAGAACCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002252 GO:0002376 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005769 GO:0005783 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0006950 GO:0006952 GO:0008150 GO:0009605 GO:0009607 GO:0009615 GO:0012505 GO:0015693 GO:0016020 GO:0030001 GO:0031410 GO:0031982 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043621 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0051179 GO:0051234 GO:0051607 GO:0051704 GO:0051707 GO:0070838 GO:0071944 GO:0072511 GO:0097708 GO:0098542

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Mg_trans_NIPA

Protein Analysis

337

Amino Acids

36.17

Weight (kDa)

6.21

Isoelectric Point (pI)

24.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Mg_trans_NIPA	PF05653	6 - 296	2.3e-135	Magnesium transporter NIPA
EamA	PF00892	8 - 122	8.8e-06	EamA-like transporter family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000984)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G21120 AT4G38730
fragaria_vesca	FvH4_1g25650 FvH4_1g25650 FvH4_1g25650 FvH4_2g01760 FvH4_2g01760 FvH4_2g01760
malus_domestica	MD00G1128200.v1.1 MD03G1168400.v1.1 MD05G1116700.v1.1 MD10G1119800.v1.1 MD15G1139500.v1.1
prunus_persica	Prupe.4G259000_v2.0.a1 Prupe.4G259000_v2.0.a1 Prupe.4G259000_v2.0.a1 Prupe.4G259000_v2.0.a1 Prupe.4G259000_v2.0.a1 Prupe.4G259000_v2.0.a1 Prupe.4G259000_v2.0.a1 Prupe.4G259000_v2.0.a1 Prupe.4G259000_v2.0.a1 Prupe.4G259000_v2.0.a1 Prupe.4G259000_v2.0.a1 Prupe.4G259000_v2.0.a1 Prupe.4G259000_v2.0.a1 Prupe.4G282600_v2.0.a1 Prupe.4G282600_v2.0.a1 Prupe.4G282600_v2.0.a1 Prupe.8G161200_v2.0.a1 Prupe.8G161200_v2.0.a1
pyrus_communis	pycom03g12470 pycom05g11100 pycom10g10320 pycom15g12590 pycom520g00770
rosa_chinensis	RchiOBHm_Chr2g0124131 RchiOBHm_Chr5g0050741 RchiOBHm_Chr6g0244791
rosa_laevigata	RLG00000015328 RLG00000018785 RLG00000034727
rosa_multiflora	Rmu_sc0002064.1_g000008 Rmu_sc0002117.1_g000013 Rmu_sc0008812.1_g000003 Rmu_sc0036275.1_g000001
rosa_roxburghii	Rroxscaffold_2G00120110 Rroxscaffold_7G00215490
rosa_rugosa	Rorug05G0263300 Rorug05G0263400 Rorug05G0263500 Rorug05G0510800.1 Rorug05G0510900
rosa_samantha	Rh2AG307700 Rh2BG316700 Rh5BG344900 Rh5CG372800 Rh5DG358000 Rh6BG021600
rosa_wichuraiana	Rw2G024800 Rw5G031660 Rw6G002190

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	347
AccB1I	GGYRCC	1 cut(s)	113
AccB7I	CCANNNNNTGG	1 cut(s)	503
AccI	GTMKAC	1 cut(s)	525
AccII	CGCG	2 cut(s)	65, 99
AciI	CCGC	1 cut(s)	108
AclI	AACGTT	1 cut(s)	970
AclWI	GGATC	2 cut(s)	358, 361
AcsI	RAATTY	2 cut(s)	394, 988
AcuI	CTGAAG	1 cut(s)	981
AcyI	GRCGYC	1 cut(s)	44
AdeI	CACNNNGTG	1 cut(s)	251
AfaI	GTAC	1 cut(s)	932
AfiI	CCNNNNNNNGG	1 cut(s)	503
AgsI	TTSAA	4 cut(s)	298, 494, 715, 790
AhlI	ACTAGT	1 cut(s)	520
AjiI	CACGTC	1 cut(s)	639
AjnI	CCWGG	1 cut(s)	417
AjuI	GAANNNNNNNTTGG	2 cut(s)	420, 452
AleI	CACNNNNGTG	1 cut(s)	160
AluBI	AGCT	3 cut(s)	69, 455, 598
AluI	AGCT	3 cut(s)	69, 455, 598
Alw21I	GWGCWC	1 cut(s)	256
Alw26I	GTCTC	1 cut(s)	95
Alw44I	GTGCAC	1 cut(s)	252
AlwI	GGATC	2 cut(s)	358, 361
Ama87I	CYCGRG	1 cut(s)	117
AoxI	GGCC	2 cut(s)	105, 210
ApaLI	GTGCAC	1 cut(s)	252
ApeKI	GCWGC	2 cut(s)	719, 816
ApoI	RAATTY	2 cut(s)	394, 988
Asp700I	GAANNNNTTC	1 cut(s)	538
AspLEI	GCGC	3 cut(s)	65, 99, 101
AspS9I	GGNCC	2 cut(s)	105, 415
AsuC2I	CCSGG	2 cut(s)	103, 734
AsuNHI	GCTAGC	1 cut(s)	773
AvaI	CYCGRG	1 cut(s)	117
AvaII	GGWCC	1 cut(s)	415
AxyI	CCTNAGG	1 cut(s)	378
BaeGI	GKGCMC	1 cut(s)	256
BanI	GGYRCC	1 cut(s)	113
Bbv12I	GWGCWC	1 cut(s)	256
BbvI	GCAGC	2 cut(s)	731, 828
BccI	CCATC	4 cut(s)	34, 541, 872, 904
BciT130I	CCWGG	1 cut(s)	419
BciVI	GTATCC	1 cut(s)	700
BclI	TGATCA	1 cut(s)	174
BcnI	CCSGG	2 cut(s)	103, 734
BcoDI	GTCTC	1 cut(s)	95
BcuI	ACTAGT	1 cut(s)	520
BfaI	CTAG	3 cut(s)	467, 521, 774
BfmI	CTRYAG	1 cut(s)	399
BfuAI	ACCTGC	1 cut(s)	347
BfuI	GTATCC	1 cut(s)	700
BisI	GCNGC	3 cut(s)	108, 720, 817
BlsI	GCNGC	3 cut(s)	109, 721, 818
Bme1390I	CCNGG	3 cut(s)	103, 419, 734
Bme18I	GGWCC	1 cut(s)	415
BmeT110I	CYCGRG	1 cut(s)	117
BmgBI	CACGTC	1 cut(s)	639
BmgT120I	GGNCC	2 cut(s)	105, 415
BmiI	GGNNCC	5 cut(s)	106, 115, 416, 862, 894
BmrFI	CCNGG	3 cut(s)	103, 419, 734
BmsI	GCATC	1 cut(s)	351
BmtI	GCTAGC	1 cut(s)	777
BpuEI	CTTGAG	2 cut(s)	77, 626
BpuMI	CCSGG	2 cut(s)	103, 734
BsaBI	GATNNNNATC	1 cut(s)	365
BsaHI	GRCGYC	1 cut(s)	44
BsaI	GGTCTC	1 cut(s)	95
BsaJI	CCNNGG	2 cut(s)	102, 732
BsaXI	ACNNNNNCTCC	2 cut(s)	933, 963
Bsc4I	CCNNNNNNNGG	1 cut(s)	503
Bse118I	RCCGGY	1 cut(s)	110
Bse1I	ACTGG	1 cut(s)	39
Bse21I	CCTNAGG	1 cut(s)	378
Bse8I	GATNNNNATC	1 cut(s)	365
BseBI	CCWGG	1 cut(s)	419
BseDI	CCNNGG	2 cut(s)	102, 732
BseGI	GGATG	2 cut(s)	72, 335
BseJI	GATNNNNATC	1 cut(s)	365
BseLI	CCNNNNNNNGG	1 cut(s)	503
BseMII	CTCAG	3 cut(s)	248, 392, 647
BseNI	ACTGG	1 cut(s)	39
BsePI	GCGCGC	1 cut(s)	97
BseRI	GAGGAG	1 cut(s)	954
BseSI	GKGCMC	1 cut(s)	256
BseXI	GCAGC	2 cut(s)	731, 828
Bsh1236I	CGCG	2 cut(s)	65, 99
BshFI	GGCC	2 cut(s)	107, 212
BshNI	GGYRCC	1 cut(s)	113
BsiHKAI	GWGCWC	1 cut(s)	256
BsiHKCI	CYCGRG	1 cut(s)	117
BsiSI	CCGG	4 cut(s)	102, 111, 227, 734
BslI	CCNNNNNNNGG	1 cut(s)	503
BsmAI	GTCTC	1 cut(s)	95
BsmI	GAATGC	1 cut(s)	22
BsnI	GGCC	2 cut(s)	107, 212
Bso31I	GGTCTC	1 cut(s)	95
BsoBI	CYCGRG	1 cut(s)	117
Bsp1286I	GDGCHC	1 cut(s)	256
Bsp143I	GATC	5 cut(s)	174, 350, 360, 366, 886
BspACI	CCGC	1 cut(s)	108
BspANI	GGCC	2 cut(s)	107, 212
BspCNI	CTCAG	3 cut(s)	249, 391, 646
BspFNI	CGCG	2 cut(s)	65, 99
BspLI	GGNNCC	5 cut(s)	106, 115, 416, 862, 894
BspMI	ACCTGC	1 cut(s)	347
BspOI	GCTAGC	1 cut(s)	777
BspPI	GGATC	2 cut(s)	358, 361
BspT107I	GGYRCC	1 cut(s)	113
BspTNI	GGTCTC	1 cut(s)	95
BsrFI	RCCGGY	1 cut(s)	110
BsrI	ACTGG	1 cut(s)	39
BssAI	RCCGGY	1 cut(s)	110
BssECI	CCNNGG	2 cut(s)	102, 732
BssHII	GCGCGC	1 cut(s)	97
BssMI	GATC	5 cut(s)	174, 350, 360, 366, 886
BssNI	GRCGYC	1 cut(s)	44
Bst2UI	CCWGG	1 cut(s)	419
Bst4CI	ACNGT	6 cut(s)	562, 655, 681, 850, 922, 1008
Bst6I	CTCTTC	1 cut(s)	947
BstACI	GRCGYC	1 cut(s)	44
BstAPI	GCANNNNNTGC	1 cut(s)	728
BstC8I	GCNNGC	6 cut(s)	67, 99, 112, 169, 775, 876
BstDEI	CTNAG	3 cut(s)	257, 378, 633
BstF5I	GGATG	2 cut(s)	72, 335
BstFNI	CGCG	2 cut(s)	65, 99
BstHHI	GCGC	3 cut(s)	65, 99, 101
BstKTI	GATC	5 cut(s)	177, 353, 363, 369, 889
BstMAI	GTCTC	1 cut(s)	95
BstMBI	GATC	5 cut(s)	174, 350, 360, 366, 886
BstMWI	GCNNNNNNNGC	4 cut(s)	50, 107, 281, 728
BstNI	CCWGG	1 cut(s)	419
BstNSI	RCATGY	1 cut(s)	878
BstSCI	CCNGG	3 cut(s)	101, 417, 732
BstSFI	CTRYAG	1 cut(s)	399
BstSLI	GKGCMC	1 cut(s)	256
BstUI	CGCG	2 cut(s)	65, 99
BstV1I	GCAGC	2 cut(s)	731, 828
BstXI	CCANNNNNNTGG	2 cut(s)	162, 208
Bsu36I	CCTNAGG	1 cut(s)	378
BsuI	GTATCC	1 cut(s)	700
BsuRI	GGCC	2 cut(s)	107, 212
BtgZI	GCGATG	1 cut(s)	53
BtrI	CACGTC	1 cut(s)	639
BtsCI	GGATG	2 cut(s)	72, 335
BtsIMutI	CAGTG	3 cut(s)	254, 277, 954
BveI	ACCTGC	1 cut(s)	347
Cac8I	GCNNGC	6 cut(s)	67, 99, 112, 169, 775, 876
CfoI	GCGC	3 cut(s)	65, 99, 101
Cfr10I	RCCGGY	1 cut(s)	110
Cfr13I	GGNCC	2 cut(s)	105, 415
CseI	GACGC	1 cut(s)	33
Csp6I	GTAC	1 cut(s)	931
CviAII	CATG	5 cut(s)	172, 346, 371, 875, 927
CviQI	GTAC	1 cut(s)	931
DdeI	CTNAG	3 cut(s)	257, 378, 633
DpnI	GATC	5 cut(s)	176, 352, 362, 368, 888
DpnII	GATC	5 cut(s)	174, 350, 360, 366, 886
DraIII	CACNNNGTG	1 cut(s)	251
Eam1104I	CTCTTC	1 cut(s)	947
EarI	CTCTTC	1 cut(s)	947
Eco31I	GGTCTC	1 cut(s)	95
Eco47I	GGWCC	1 cut(s)	415
Eco57I	CTGAAG	1 cut(s)	981
Eco81I	CCTNAGG	1 cut(s)	378
Eco88I	CYCGRG	1 cut(s)	117
EcoO109I	RGGNCCY	1 cut(s)	415
EcoRI	GAATTC	1 cut(s)	988
EcoRII	CCWGG	1 cut(s)	417
FaeI	CATG	5 cut(s)	175, 349, 374, 878, 930
FatI	CATG	5 cut(s)	171, 345, 370, 874, 926
FbaI	TGATCA	1 cut(s)	174
FblI	GTMKAC	1 cut(s)	525
Fnu4HI	GCNGC	3 cut(s)	108, 720, 817
FokI	GGATG	2 cut(s)	59, 342
Fsp4HI	GCNGC	3 cut(s)	108, 720, 817
FspBI	CTAG	3 cut(s)	467, 521, 774
GlaI	GCGC	3 cut(s)	64, 98, 100
GluI	GCNGC	3 cut(s)	108, 720, 817
HaeIII	GGCC	2 cut(s)	107, 212
HapII	CCGG	4 cut(s)	102, 111, 227, 734
HgaI	GACGC	1 cut(s)	33
HhaI	GCGC	3 cut(s)	65, 99, 101
Hin1I	GRCGYC	1 cut(s)	44
Hin1II	CATG	5 cut(s)	175, 349, 374, 878, 930
Hin6I	GCGC	3 cut(s)	63, 97, 99
HinP1I	GCGC	3 cut(s)	63, 97, 99
HinfI	GANTC	3 cut(s)	534, 840, 944
HpaII	CCGG	4 cut(s)	102, 111, 227, 734
Hpy166II	GTNNAC	4 cut(s)	254, 526, 554, 753
Hpy188I	TCNGA	9 cut(s)	13, 621, 636, 651, 762, 807, 829, 961, 994
Hpy188III	TCNNGA	8 cut(s)	76, 358, 364, 380, 605, 858, 890, 1001
Hpy8I	GTNNAC	4 cut(s)	254, 526, 554, 753
Hpy99I	CGWCG	1 cut(s)	49
HpyAV	CCTTC	3 cut(s)	16, 79, 292
HpyCH4III	ACNGT	6 cut(s)	562, 655, 681, 850, 922, 1008
HpyCH4IV	ACGT	2 cut(s)	638, 970
HpyCH4V	TGCA	8 cut(s)	254, 342, 374, 487, 590, 719, 874, 878
HpyF10VI	GCNNNNNNNGC	4 cut(s)	50, 107, 281, 728
HpyF3I	CTNAG	3 cut(s)	257, 378, 633
HpySE526I	ACGT	2 cut(s)	638, 970
Hsp92I	GRCGYC	1 cut(s)	44
Hsp92II	CATG	5 cut(s)	175, 349, 374, 878, 930
HspAI	GCGC	3 cut(s)	63, 97, 99
KroI	GCCGGC	1 cut(s)	110
KroNI	GCCGGC	1 cut(s)	112
Ksp22I	TGATCA	1 cut(s)	174
Kzo9I	GATC	5 cut(s)	174, 350, 360, 366, 886
LmnI	GCTCC	2 cut(s)	229, 907
Lsp1109I	GCAGC	2 cut(s)	731, 828
LweI	GCATC	1 cut(s)	351
MaeI	CTAG	3 cut(s)	467, 521, 774
MaeII	ACGT	2 cut(s)	638, 970
MaeIII	GTNAC	4 cut(s)	238, 334, 673, 922
MalI	GATC	5 cut(s)	176, 352, 362, 368, 888
MboI	GATC	5 cut(s)	174, 350, 360, 366, 886
MboII	GAAGA	2 cut(s)	964, 974
MfeI	CAATTG	2 cut(s)	579, 723
MhlI	GDGCHC	1 cut(s)	256
MnlI	CCTC	6 cut(s)	387, 397, 601, 642, 835, 932
MroNI	GCCGGC	1 cut(s)	110
MroXI	GAANNNNTTC	1 cut(s)	538
MseI	TTAA	2 cut(s)	683, 693
MslI	CAYNNNNRTG	2 cut(s)	160, 848
MspI	CCGG	4 cut(s)	102, 111, 227, 734
MspR9I	CCNGG	3 cut(s)	103, 419, 734
MunI	CAATTG	2 cut(s)	579, 723
Mva1269I	GAATGC	1 cut(s)	22
MvaI	CCWGG	1 cut(s)	419
MvnI	CGCG	2 cut(s)	65, 99
MwoI	GCNNNNNNNGC	4 cut(s)	50, 107, 281, 728
NaeI	GCCGGC	1 cut(s)	112
NciI	CCSGG	2 cut(s)	103, 734
NdeII	GATC	5 cut(s)	174, 350, 360, 366, 886
NgoMIV	GCCGGC	1 cut(s)	110
NheI	GCTAGC	1 cut(s)	773
NlaIII	CATG	5 cut(s)	175, 349, 374, 878, 930
NlaIV	GGNNCC	5 cut(s)	106, 115, 416, 862, 894
NspI	RCATGY	1 cut(s)	878
OliI	CACNNNNGTG	1 cut(s)	160
PaeI	GCATGC	1 cut(s)	878
PauI	GCGCGC	1 cut(s)	97
PcsI	WCGNNNNNNNCGW	1 cut(s)	17
PctI	GAATGC	1 cut(s)	22
PdiI	GCCGGC	1 cut(s)	112
PdmI	GAANNNNTTC	1 cut(s)	538
PfeI	GAWTC	3 cut(s)	534, 840, 944
PflMI	CCANNNNNTGG	1 cut(s)	503
PkrI	GCNGC	3 cut(s)	109, 721, 818
PpuMI	RGGWCCY	1 cut(s)	415
Psp1406I	AACGTT	1 cut(s)	970
Psp5II	RGGWCCY	1 cut(s)	415
Psp6I	CCWGG	1 cut(s)	417
PspGI	CCWGG	1 cut(s)	417
PspN4I	GGNNCC	5 cut(s)	106, 115, 416, 862, 894
PspPI	GGNCC	2 cut(s)	105, 415
PspPPI	RGGWCCY	1 cut(s)	415
PteI	GCGCGC	1 cut(s)	97
RsaI	GTAC	1 cut(s)	932
RsaNI	GTAC	1 cut(s)	931
RseI	CAYNNNNRTG	2 cut(s)	160, 848
SaqAI	TTAA	2 cut(s)	683, 693
SatI	GCNGC	3 cut(s)	108, 720, 817
Sau3AI	GATC	5 cut(s)	174, 350, 360, 366, 886
Sau96I	GGNCC	2 cut(s)	105, 415
ScrFI	CCNGG	3 cut(s)	103, 419, 734
SduI	GDGCHC	1 cut(s)	256
SetI	ASST	7 cut(s)	71, 341, 379, 457, 600, 641, 973
SfaNI	GCATC	1 cut(s)	351
SfcI	CTRYAG	1 cut(s)	399
SinI	GGWCC	1 cut(s)	415
SmiMI	CAYNNNNRTG	2 cut(s)	160, 848
SmlI	CTYRAG	2 cut(s)	92, 605
SmoI	CTYRAG	2 cut(s)	92, 605
SpeI	ACTAGT	1 cut(s)	520
SphI	GCATGC	1 cut(s)	878
SsiI	CCGC	1 cut(s)	108
SspMI	CTAG	3 cut(s)	467, 521, 774
StyD4I	CCNGG	3 cut(s)	101, 417, 732
TaaI	ACNGT	6 cut(s)	562, 655, 681, 850, 922, 1008
TaiI	ACGT	2 cut(s)	641, 973
TaqI	TCGA	1 cut(s)	47
TauI	GCSGC	1 cut(s)	110
TfiI	GAWTC	3 cut(s)	534, 840, 944
Tru1I	TTAA	2 cut(s)	683, 693
Tru9I	TTAA	2 cut(s)	683, 693
TscAI	CASTG	3 cut(s)	261, 277, 954
TseI	GCWGC	2 cut(s)	719, 816
TspDTI	ATGAA	4 cut(s)	46, 61, 832, 981
TspRI	CASTG	3 cut(s)	261, 277, 954
Van91I	CCANNNNNTGG	1 cut(s)	503
VneI	GTGCAC	1 cut(s)	252
VpaK11BI	GGWCC	1 cut(s)	415
XapI	RAATTY	2 cut(s)	394, 988
XceI	RCATGY	1 cut(s)	878
XmiI	GTMKAC	1 cut(s)	525
XmnI	GAANNNNTTC	1 cut(s)	538
XspI	CTAG	3 cut(s)	467, 521, 774

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.