Rroxscaffold_2G00120110

Acts as a Mg(2 ) transporter. Can also transport other divalent cations such as Fe(2 ), Sr(2 ), Ba(2 ), Mn(2 ) and Co(2 ) but to a much less extent than Mg(2 )

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000984
Physical Location & Seq
Reverse (-)
52187252 .. 52192213
4962 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00120110.1

Sequence Viewer

Length: 699 bp
ATGCATAGTGGGTTCGGTTGTGATTGTACTCCATGCACCCGAAGAGCAGACGCTTACTTCCGTCCAACAAATCTGGGATTTAGCAATACAACCAGCTTTCCTTTTGTATACAGCCTCAGTGATAGCTGTAACGCTAGTCCTTATCTTGTATTGTTCCCCCGCTATGGCCAAACCAATATACTGATTTATATAGGAATATGCTCAATTATTGGATCATTGACTGTCATGAGCATAAAAGCAATTGGCATTGCAATAAAACTCACAATAGAGGGCACAAACCAGGCAGTATACTTCCAAACATGGATTTTTGCAATGGTTGCAGTTACATGTATCATCACTCAATTAAATTATCTAAATATGGCATTGGACACTTTCAACACAGCAGTCGTTTCTCCCATCTATTATGCTATGTTCACATCTTTCACAATACTTGCTAGCGCAATAATGTTCAAGGATTATTCTGGTCAAAGTGCAAGCAGCATAGCTTCAGAATTTTGTGGATTCATCACTGTGTTATCTGGGACTGCTATATTGCATAGTACAAGAGAACCAGATCCACCTTTACTTACAGATTTGTATACACCATTATCTCCTAAAGTTTCGTGGTACATCCAAAGTAATGGAGAATGGAAGCAGAAGGATGAAGACGGTTCAGCCCCCAATTTCATCACAGTACTTAGGCCAGACTATTTCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mg_trans_NIPA

Protein Analysis

232

Amino Acids

25.65

Weight (kDa)

5.59

Isoelectric Point (pI)

37.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mg_trans_NIPA PF05653 49 - 183 2.5e-55 Magnesium transporter NIPA
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 3 cut(s) 108, 288, 578
AciI CCGC 1 cut(s) 160
AclWI GGATC 2 cut(s) 220, 548
AcoI YGGCCR 1 cut(s) 166
AcsI RAATTY 1 cut(s) 491
AcuI CTGAAG 1 cut(s) 471
AfaI GTAC 4 cut(s) 28, 541, 608, 675
AfiI CCNNNNNNNGG 1 cut(s) 164
AflIII ACRYGT 1 cut(s) 326
AgsI TTSAA 3 cut(s) 376, 451, 694
AjnI CCWGG 1 cut(s) 279
AluBI AGCT 3 cut(s) 96, 126, 485
AluI AGCT 3 cut(s) 96, 126, 485
AlwI GGATC 2 cut(s) 220, 548
AoxI GGCC 2 cut(s) 166, 680
ApeKI GCWGC 1 cut(s) 477
ApoI RAATTY 1 cut(s) 491
AspLEI GCGC 1 cut(s) 440
AsuNHI GCTAGC 1 cut(s) 434
BaeGI GKGCMC 1 cut(s) 275
BalI TGGCCA 1 cut(s) 168
BbsI GAAGAC 1 cut(s) 651
BbvI GCAGC 1 cut(s) 489
BccI CCATC 1 cut(s) 404
BciT130I CCWGG 1 cut(s) 281
BfaI CTAG 2 cut(s) 135, 435
BisI GCNGC 1 cut(s) 478
BlsI GCNGC 1 cut(s) 479
BmcAI AGTACT 1 cut(s) 675
Bme1390I CCNGG 1 cut(s) 281
BmrFI CCNGG 1 cut(s) 281
BmtI GCTAGC 1 cut(s) 438
BpiI GAAGAC 1 cut(s) 651
Bsc4I CCNNNNNNNGG 1 cut(s) 164
Bse3DI GCAATG 2 cut(s) 246, 318
BseBI CCWGG 1 cut(s) 281
BseGI GGATG 2 cut(s) 609, 646
BseLI CCNNNNNNNGG 1 cut(s) 164
BseMI GCAATG 2 cut(s) 246, 318
BseMII CTCAG 1 cut(s) 130
BseSI GKGCMC 1 cut(s) 275
BseXI GCAGC 1 cut(s) 489
BshFI GGCC 2 cut(s) 168, 682
BslFI GGGAC 1 cut(s) 535
BslI CCNNNNNNNGG 1 cut(s) 164
BsmFI GGGAC 1 cut(s) 535
BsnI GGCC 2 cut(s) 168, 682
Bsp1286I GDGCHC 1 cut(s) 275
Bsp143I GATC 2 cut(s) 212, 553
BspACI CCGC 1 cut(s) 160
BspANI GGCC 2 cut(s) 168, 682
BspCNI CTCAG 1 cut(s) 129
BspHI TCATGA 1 cut(s) 225
BspOI GCTAGC 1 cut(s) 438
BspPI GGATC 2 cut(s) 220, 548
BspQI GCTCTTC 1 cut(s) 37
BsrDI GCAATG 2 cut(s) 246, 318
BssMI GATC 2 cut(s) 212, 553
BssNAI GTATAC 3 cut(s) 109, 289, 579
Bst1107I GTATAC 3 cut(s) 109, 289, 579
Bst2UI CCWGG 1 cut(s) 281
Bst4CI ACNGT 4 cut(s) 223, 511, 650, 673
Bst6I CTCTTC 1 cut(s) 37
BstAPI GCANNNNNTGC 1 cut(s) 317
BstC8I GCNNGC 2 cut(s) 436, 475
BstDEI CTNAG 2 cut(s) 116, 677
BstF5I GGATG 2 cut(s) 609, 646
BstHHI GCGC 1 cut(s) 440
BstKTI GATC 2 cut(s) 215, 556
BstMBI GATC 2 cut(s) 212, 553
BstMWI GCNNNNNNNGC 1 cut(s) 317
BstNI CCWGG 1 cut(s) 281
BstNSI RCATGY 1 cut(s) 330
BstSCI CCNGG 1 cut(s) 279
BstSLI GKGCMC 1 cut(s) 275
BstV1I GCAGC 1 cut(s) 489
BstV2I GAAGAC 1 cut(s) 651
BstX2I RGATCY 1 cut(s) 553
BstXI CCANNNNNNTGG 1 cut(s) 620
BstYI RGATCY 1 cut(s) 553
BstZ17I GTATAC 3 cut(s) 109, 289, 579
BsuRI GGCC 2 cut(s) 168, 682
BtsCI GGATG 2 cut(s) 609, 646
BtsIMutI CAGTG 2 cut(s) 124, 507
Cac8I GCNNGC 2 cut(s) 436, 475
CciI TCATGA 1 cut(s) 225
CfoI GCGC 1 cut(s) 440
CseI GACGC 1 cut(s) 59
Csp6I GTAC 4 cut(s) 27, 540, 607, 674
CviAII CATG 4 cut(s) 33, 226, 300, 327
CviJI RGCY 7 cut(s) 96, 114, 126, 168, 485, 656, 682
CviKI_1 RGCY 7 cut(s) 96, 114, 126, 168, 485, 656, 682
CviQI GTAC 4 cut(s) 27, 540, 607, 674
DdeI CTNAG 2 cut(s) 116, 677
DpnI GATC 2 cut(s) 214, 555
DpnII GATC 2 cut(s) 212, 553
EaeI YGGCCR 1 cut(s) 166
Eam1104I CTCTTC 1 cut(s) 37
EarI CTCTTC 1 cut(s) 37
Eco57I CTGAAG 1 cut(s) 471
EcoRII CCWGG 1 cut(s) 279
EcoT22I ATGCAT 1 cut(s) 6
FaeI CATG 4 cut(s) 36, 229, 303, 330
FaqI GGGAC 1 cut(s) 535
FatI CATG 4 cut(s) 32, 225, 299, 326
FauI CCCGC 1 cut(s) 167
FblI GTMKAC 3 cut(s) 108, 288, 578
Fnu4HI GCNGC 1 cut(s) 478
FokI GGATG 2 cut(s) 596, 653
Fsp4HI GCNGC 1 cut(s) 478
FspBI CTAG 2 cut(s) 135, 435
GlaI GCGC 1 cut(s) 439
GluI GCNGC 1 cut(s) 478
HaeIII GGCC 2 cut(s) 168, 682
HgaI GACGC 1 cut(s) 59
HhaI GCGC 1 cut(s) 440
Hin1II CATG 4 cut(s) 36, 229, 303, 330
Hin6I GCGC 1 cut(s) 438
HinP1I GCGC 1 cut(s) 438
HinfI GANTC 1 cut(s) 501
Hpy166II GTNNAC 4 cut(s) 109, 289, 414, 579
Hpy188I TCNGA 1 cut(s) 490
Hpy188III TCNNGA 1 cut(s) 226
Hpy8I GTNNAC 4 cut(s) 109, 289, 414, 579
HpyAV CCTTC 1 cut(s) 631
HpyCH4III ACNGT 4 cut(s) 223, 511, 650, 673
HpyCH4V TGCA 7 cut(s) 4, 36, 251, 311, 320, 473, 535
HpyF10VI GCNNNNNNNGC 1 cut(s) 317
HpyF3I CTNAG 2 cut(s) 116, 677
Hsp92II CATG 4 cut(s) 36, 229, 303, 330
HspAI GCGC 1 cut(s) 438
Kzo9I GATC 2 cut(s) 212, 553
LguI GCTCTTC 1 cut(s) 37
LpnPI CCDG 7 cut(s) 59, 106, 266, 293, 447, 504, 564
Lsp1109I GCAGC 1 cut(s) 489
MaeI CTAG 2 cut(s) 135, 435
MaeIII GTNAC 2 cut(s) 128, 322
MalI GATC 2 cut(s) 214, 555
MboI GATC 2 cut(s) 212, 553
MboII GAAGA 2 cut(s) 54, 656
MfeI CAATTG 1 cut(s) 240
MflI RGATCY 1 cut(s) 553
MhlI GDGCHC 1 cut(s) 275
MlsI TGGCCA 1 cut(s) 168
MluCI AATT 6 cut(s) 204, 240, 341, 346, 491, 661
MluNI TGGCCA 1 cut(s) 168
MmeI TCCRAC 1 cut(s) 89
MnlI CCTC 2 cut(s) 125, 262
Mox20I TGGCCA 1 cut(s) 168
Mph1103I ATGCAT 1 cut(s) 6
MscI TGGCCA 1 cut(s) 168
MseI TTAA 1 cut(s) 344
MslI CAYNNNNRTG 1 cut(s) 509
Msp20I TGGCCA 1 cut(s) 168
MspR9I CCNGG 1 cut(s) 281
MunI CAATTG 1 cut(s) 240
MvaI CCWGG 1 cut(s) 281
MwoI GCNNNNNNNGC 1 cut(s) 317
NdeII GATC 2 cut(s) 212, 553
NheI GCTAGC 1 cut(s) 434
NlaIII CATG 4 cut(s) 36, 229, 303, 330
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 330
PagI TCATGA 1 cut(s) 225
PciI ACATGT 1 cut(s) 326
PciSI GCTCTTC 1 cut(s) 37
PfeI GAWTC 1 cut(s) 501
PkrI GCNGC 1 cut(s) 479
PscI ACATGT 1 cut(s) 326
Psp6I CCWGG 1 cut(s) 279
PspGI CCWGG 1 cut(s) 279
PsuI RGATCY 1 cut(s) 553
RsaI GTAC 4 cut(s) 28, 541, 608, 675
RsaNI GTAC 4 cut(s) 27, 540, 607, 674
RseI CAYNNNNRTG 1 cut(s) 509
SapI GCTCTTC 1 cut(s) 37
SaqAI TTAA 1 cut(s) 344
SatI GCNGC 1 cut(s) 478
Sau3AI GATC 2 cut(s) 212, 553
ScaI AGTACT 1 cut(s) 675
ScrFI CCNGG 1 cut(s) 281
SduI GDGCHC 1 cut(s) 275
SetI ASST 4 cut(s) 98, 128, 487, 562
SmiMI CAYNNNNRTG 1 cut(s) 509
Sse9I AATT 6 cut(s) 204, 240, 341, 346, 491, 661
SsiI CCGC 1 cut(s) 160
SspMI CTAG 2 cut(s) 135, 435
StyD4I CCNGG 1 cut(s) 279
TaaI ACNGT 4 cut(s) 223, 511, 650, 673
TasI AATT 6 cut(s) 204, 240, 341, 346, 491, 661
TatI WGTACW 3 cut(s) 26, 539, 673
TfiI GAWTC 1 cut(s) 501
Tru1I TTAA 1 cut(s) 344
Tru9I TTAA 1 cut(s) 344
TscAI CASTG 2 cut(s) 124, 514
TseI GCWGC 1 cut(s) 477
TspDTI ATGAA 3 cut(s) 493, 655, 657
TspGWI ACGGA 1 cut(s) 50
TspRI CASTG 2 cut(s) 124, 514
XapI RAATTY 1 cut(s) 491
XceI RCATGY 1 cut(s) 330
XmiI GTMKAC 3 cut(s) 108, 288, 578
XspI CTAG 2 cut(s) 135, 435
ZrmI AGTACT 1 cut(s) 675
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.