Rh5DG358000

Acts as a Mg(2 ) transporter. Can also transport other divalent cations such as Fe(2 ), Sr(2 ), Ba(2 ), Mn(2 ) and Co(2 ) but to a much less extent than Mg(2 )

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000984
Physical Location & Seq
Forward (+)
53338013 .. 53343705
5693 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG358000.1

Sequence Viewer

Length: 957 bp
ATGGGTTTCTCCAAAGACAATCTTAAAGGCCTTCTTCTAGCTCTCCTATCAAGTGGGTTCATAGGGGCAAGCTTCATCATCAAGAAAAAAGGCCTCCGAAGAGCCGCAGCTGCTTCTGGGATCAGAGCTGGCTATGGTGGGTATTCTTATCTCTTGGAGCCTCTATGGTGGCTGGGATTGATAACAATGGTTGTGGGAGAGGTTGCGAATTTCATTGCATATGCATTTGCTCCAGCAGTGCTTGTTACCCCTCTTGGTGCATTAAGTATAATTATCAGTGCTGTGCTGGCTCATTTTATCCTGAAGGAGAGGTTGCACTGGCTCGGGGTTTTGGGCTGTGTAATGTGCATTTCTGGATCGGTCATAATCGTTATCCATGCACCACAGGAGCATCCTATCACCAATGTTCTTGAAATATGGAGTATGGCAACTCAACCAGCTTTCATGCTTTATGTGGGCTCGGTGATTGTTCTGGTTTTCATACTAATCTTCCATTTTGTGCCACGATGTGGGCAAACAAATGTGCTAGTTTTCACTGGCATCTGTTCATTGATGGGTTCTCTATCAGTAATGAGTGTTAAAGCCCTAGGAACTGCTCTAAAATTAACTTTTGAGGGAAAGAATCAGTTAATCTATCCAGAGACATGGTTTTTTATGTTGGTTGTAACTACATGTGTCATCACTCAGATGAACTATCTGAACAAGGATTGGGATGACCAAAGTGGAGGAGCGATCATATCAGAAATATGTGGCTTTATTGTTGTATTATCTGGAACCATCTTATTGCATTCAACAAAAGAATTTGAGAGAAGCTCATCCTTTAGAGGTAGCTATATGTCTGTATCCCCTTCATTATCTGCACGTCTCTGTAGTGGGAATGGAGACTCATTGAAACACGGTGAAAATGGAGGAGAGTCGTTTTCTGAGGAAATTTCATTAAGACAAGAATTGTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mg_trans_NIPA

Protein Analysis

318

Amino Acids

34.51

Weight (kDa)

7.62

Isoelectric Point (pI)

29.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mg_trans_NIPA PF05653 6 - 236 4.3e-100 Magnesium transporter NIPA
EamA PF00892 9 - 124 8.9e-07 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 509
AciI CCGC 1 cut(s) 105
AclWI GGATC 2 cut(s) 128, 364
AcsI RAATTY 3 cut(s) 208, 800, 930
AcuI CTGAAG 1 cut(s) 323
AdeI CACNNNGTG 1 cut(s) 509
AfaI GTAC 1 cut(s) 953
AfiI CCNNNNNNNGG 1 cut(s) 509
AflIII ACRYGT 1 cut(s) 671
AgsI TTSAA 3 cut(s) 413, 792, 892
AjiI CACGTC 1 cut(s) 863
AluBI AGCT 7 cut(s) 41, 72, 110, 128, 440, 813, 831
AluI AGCT 7 cut(s) 41, 72, 110, 128, 440, 813, 831
Alw26I GTCTC 3 cut(s) 635, 869, 876
AlwI GGATC 2 cut(s) 128, 364
Ama87I CYCGRG 1 cut(s) 323
AoxI GGCC 2 cut(s) 28, 91
ApeKI GCWGC 2 cut(s) 107, 110
ApoI RAATTY 3 cut(s) 208, 800, 930
AspA2I CCTAGG 1 cut(s) 586
AsuHPI GGTGA 3 cut(s) 391, 475, 911
AvaI CYCGRG 1 cut(s) 323
AvrII CCTAGG 1 cut(s) 586
BanII GRGCYC 1 cut(s) 461
BbvI GCAGC 2 cut(s) 97, 119
BccI CCATC 2 cut(s) 547, 785
BciVI GTATCC 1 cut(s) 853
BcoDI GTCTC 3 cut(s) 635, 869, 876
BfaI CTAG 4 cut(s) 38, 527, 587, 955
BfmI CTRYAG 1 cut(s) 868
BfuI GTATCC 1 cut(s) 853
BisI GCNGC 3 cut(s) 105, 108, 111
BlnI CCTAGG 1 cut(s) 586
BlsI GCNGC 3 cut(s) 106, 109, 112
BmeT110I CYCGRG 1 cut(s) 323
BmgBI CACGTC 1 cut(s) 863
BmiI GGNNCC 2 cut(s) 159, 775
BmsI GCATC 2 cut(s) 400, 549
BplI GAGNNNNNCTC 2 cut(s) 797, 829
BpmI CTGGAG 1 cut(s) 216
BsaJI CCNNGG 1 cut(s) 586
Bsc4I CCNNNNNNNGG 1 cut(s) 509
Bse1I ACTGG 2 cut(s) 323, 541
Bse3DI GCAATG 1 cut(s) 213
BseDI CCNNGG 1 cut(s) 586
BseGI GGATG 3 cut(s) 391, 718, 815
BseLI CCNNNNNNNGG 1 cut(s) 509
BseMI GCAATG 1 cut(s) 213
BseMII CTCAG 2 cut(s) 698, 915
BseNI ACTGG 2 cut(s) 323, 541
BseRI GAGGAG 2 cut(s) 741, 924
BseXI GCAGC 2 cut(s) 97, 119
BseYI CCCAGC 1 cut(s) 172
BsgI GTGCAG 1 cut(s) 843
BshFI GGCC 2 cut(s) 30, 93
BsiHKCI CYCGRG 1 cut(s) 323
BslI CCNNNNNNNGG 1 cut(s) 509
BsmAI GTCTC 3 cut(s) 635, 869, 876
BsmBI CGTCTC 1 cut(s) 869
BsmI GAATGC 1 cut(s) 787
BsnI GGCC 2 cut(s) 30, 93
BsoBI CYCGRG 1 cut(s) 323
Bsp1286I GDGCHC 1 cut(s) 461
Bsp143I GATC 3 cut(s) 120, 356, 732
BspACI CCGC 1 cut(s) 105
BspANI GGCC 2 cut(s) 30, 93
BspCNI CTCAG 2 cut(s) 697, 916
BspLI GGNNCC 2 cut(s) 159, 775
BspPI GGATC 2 cut(s) 128, 364
BspQI GCTCTTC 1 cut(s) 94
BsrDI GCAATG 1 cut(s) 213
BsrI ACTGG 2 cut(s) 323, 541
BssECI CCNNGG 1 cut(s) 586
BssMI GATC 3 cut(s) 120, 356, 732
BssT1I CCWWGG 1 cut(s) 586
Bst4CI ACNGT 1 cut(s) 899
Bst6I CTCTTC 1 cut(s) 94
BstC8I GCNNGC 3 cut(s) 70, 130, 288
BstDEI CTNAG 2 cut(s) 684, 924
BstF5I GGATG 3 cut(s) 391, 718, 815
BstKTI GATC 3 cut(s) 123, 359, 735
BstMAI GTCTC 3 cut(s) 635, 869, 876
BstMBI GATC 3 cut(s) 120, 356, 732
BstMWI GCNNNNNNNGC 2 cut(s) 110, 287
BstNSI RCATGY 1 cut(s) 675
BstSFI CTRYAG 1 cut(s) 868
BstV1I GCAGC 2 cut(s) 97, 119
BstXI CCANNNNNNTGG 1 cut(s) 645
BsuI GTATCC 1 cut(s) 853
BsuRI GGCC 2 cut(s) 30, 93
BtrI CACGTC 1 cut(s) 863
BtsCI GGATG 3 cut(s) 391, 718, 815
BtsI GCAGTG 1 cut(s) 243
BtsIMutI CAGTG 4 cut(s) 243, 283, 316, 534
Cac8I GCNNGC 3 cut(s) 70, 130, 288
Csp6I GTAC 1 cut(s) 952
CspCI CAANNNNNGTGG 2 cut(s) 174, 209
CviAII CATG 4 cut(s) 377, 445, 645, 672
CviQI GTAC 1 cut(s) 952
DdeI CTNAG 2 cut(s) 684, 924
DpnI GATC 3 cut(s) 122, 358, 734
DpnII GATC 3 cut(s) 120, 356, 732
DraIII CACNNNGTG 1 cut(s) 509
Eam1104I CTCTTC 1 cut(s) 94
EarI CTCTTC 1 cut(s) 94
Eco130I CCWWGG 1 cut(s) 586
Eco147I AGGCCT 2 cut(s) 30, 93
Eco24I GRGCYC 1 cut(s) 461
Eco57I CTGAAG 1 cut(s) 323
Eco88I CYCGRG 1 cut(s) 323
EcoT14I CCWWGG 1 cut(s) 586
EcoT22I ATGCAT 1 cut(s) 226
EcoT38I GRGCYC 1 cut(s) 461
ErhI CCWWGG 1 cut(s) 586
Esp3I CGTCTC 1 cut(s) 869
FaeI CATG 4 cut(s) 380, 448, 648, 675
FalI AAGNNNNNCTT 3 cut(s) 38, 18, 50
FatI CATG 4 cut(s) 376, 444, 644, 671
FauNDI CATATG 1 cut(s) 220
Fnu4HI GCNGC 3 cut(s) 105, 108, 111
FokI GGATG 3 cut(s) 378, 725, 802
FriOI GRGCYC 1 cut(s) 461
Fsp4HI GCNGC 3 cut(s) 105, 108, 111
FspBI CTAG 4 cut(s) 38, 527, 587, 955
GluI GCNGC 3 cut(s) 105, 108, 111
GsaI CCCAGC 1 cut(s) 176
GsuI CTGGAG 1 cut(s) 216
HaeIII GGCC 2 cut(s) 30, 93
Hin1II CATG 4 cut(s) 380, 448, 648, 675
HindIII AAGCTT 1 cut(s) 70
HinfI GANTC 3 cut(s) 622, 884, 914
HphI GGTGA 3 cut(s) 391, 475, 911
Hpy188I TCNGA 6 cut(s) 98, 125, 687, 699, 742, 925
Hpy188III TCNNGA 6 cut(s) 82, 301, 354, 410, 638, 771
HpyAV CCTTC 3 cut(s) 41, 298, 858
HpyCH4III ACNGT 1 cut(s) 899
HpyCH4IV ACGT 1 cut(s) 862
HpyCH4V TGCA 8 cut(s) 218, 224, 260, 316, 348, 380, 787, 860
HpyF10VI GCNNNNNNNGC 2 cut(s) 110, 287
HpyF3I CTNAG 2 cut(s) 684, 924
HpySE526I ACGT 1 cut(s) 862
Hsp92II CATG 4 cut(s) 380, 448, 648, 675
Kzo9I GATC 3 cut(s) 120, 356, 732
LguI GCTCTTC 1 cut(s) 94
LmnI GCTCC 4 cut(s) 157, 235, 388, 728
Lsp1109I GCAGC 2 cut(s) 97, 119
LweI GCATC 2 cut(s) 400, 549
MaeI CTAG 4 cut(s) 38, 527, 587, 955
MaeII ACGT 1 cut(s) 862
MaeIII GTNAC 2 cut(s) 244, 664
MalI GATC 3 cut(s) 122, 358, 734
MboI GATC 3 cut(s) 120, 356, 732
MboII GAAGA 3 cut(s) 26, 111, 481
MhlI GDGCHC 1 cut(s) 461
MluCI AATT 6 cut(s) 208, 270, 602, 800, 930, 947
MlyI GAGTC 2 cut(s) 878, 923
Mph1103I ATGCAT 1 cut(s) 226
MseI TTAA 6 cut(s) 24, 263, 579, 605, 629, 938
MslI CAYNNNNRTG 1 cut(s) 686
MspA1I CMGCKG 1 cut(s) 110
Mva1269I GAATGC 1 cut(s) 787
MwoI GCNNNNNNNGC 2 cut(s) 110, 287
NdeI CATATG 1 cut(s) 220
NdeII GATC 3 cut(s) 120, 356, 732
NlaIII CATG 4 cut(s) 380, 448, 648, 675
NlaIV GGNNCC 2 cut(s) 159, 775
NsiI ATGCAT 1 cut(s) 226
NspI RCATGY 1 cut(s) 675
PceI AGGCCT 2 cut(s) 30, 93
PciI ACATGT 1 cut(s) 671
PciSI GCTCTTC 1 cut(s) 94
PctI GAATGC 1 cut(s) 787
PfeI GAWTC 1 cut(s) 622
PflMI CCANNNNNTGG 1 cut(s) 509
PkrI GCNGC 3 cut(s) 106, 109, 112
PleI GAGTC 2 cut(s) 878, 922
PpsI GAGTC 2 cut(s) 878, 922
PscI ACATGT 1 cut(s) 671
PspFI CCCAGC 1 cut(s) 172
PspN4I GGNNCC 2 cut(s) 159, 775
PvuII CAGCTG 1 cut(s) 110
RsaI GTAC 1 cut(s) 953
RsaNI GTAC 1 cut(s) 952
RseI CAYNNNNRTG 1 cut(s) 686
SapI GCTCTTC 1 cut(s) 94
SaqAI TTAA 6 cut(s) 24, 263, 579, 605, 629, 938
SatI GCNGC 3 cut(s) 105, 108, 111
Sau3AI GATC 3 cut(s) 120, 356, 732
SchI GAGTC 2 cut(s) 878, 923
SduI GDGCHC 1 cut(s) 461
SfaNI GCATC 2 cut(s) 400, 549
SfcI CTRYAG 1 cut(s) 868
SmiMI CAYNNNNRTG 1 cut(s) 686
Sse9I AATT 6 cut(s) 208, 270, 602, 800, 930, 947
SseBI AGGCCT 2 cut(s) 30, 93
SsiI CCGC 1 cut(s) 105
SspMI CTAG 4 cut(s) 38, 527, 587, 955
StuI AGGCCT 2 cut(s) 30, 93
StyI CCWWGG 1 cut(s) 586
TaaI ACNGT 1 cut(s) 899
TaiI ACGT 1 cut(s) 865
TaqII GACCGA 1 cut(s) 349
TasI AATT 6 cut(s) 208, 270, 602, 800, 930, 947
TatI WGTACW 1 cut(s) 951
TauI GCSGC 1 cut(s) 107
TfiI GAWTC 1 cut(s) 622
Tru1I TTAA 6 cut(s) 24, 263, 579, 605, 629, 938
Tru9I TTAA 6 cut(s) 24, 263, 579, 605, 629, 938
TscAI CASTG 4 cut(s) 243, 283, 323, 541
TseI GCWGC 2 cut(s) 107, 110
TspDTI ATGAA 9 cut(s) 49, 64, 202, 433, 469, 537, 704, 840, 924
TspRI CASTG 4 cut(s) 243, 283, 323, 541
Van91I CCANNNNNTGG 1 cut(s) 509
XapI RAATTY 3 cut(s) 208, 800, 930
XceI RCATGY 1 cut(s) 675
XmaJI CCTAGG 1 cut(s) 586
XspI CTAG 4 cut(s) 38, 527, 587, 955
Zsp2I ATGCAT 1 cut(s) 226
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.