MD00G1128200.v1.1

Acts as a Mg(2 ) transporter. Can also transport other divalent cations such as Fe(2 ), Sr(2 ), Ba(2 ), Mn(2 ) and Co(2 ) but to a much less extent than Mg(2 )

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0000984
Physical Location & Seq
Forward (+)
27238090 .. 27244294
6205 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1128200.v1.1.491

Sequence Viewer

Length: 1035 bp
ATGTACTCAAGCAATTTGAAAGGCTTCATCTTGGCCGTCGTCTCGAGCGCCTTCATTGGCTCCAGCTTCATCATCAAGAAGAAGGGTCTCCGCCGGGCCGGCGTCAACGGCCCTCGAGCCAGTGTGGGAGGATATGGGTATCTGTTAGAGCCTCTATGGTGGATAGGAATGGTAACAATGGTTGTTGGAGAGATAGCCAATTTTGTAGCATACATTTACGCACCTGCTGGCCTGGTCACCCCGCTTGGTGCATTGAGCATTATTGTTAGTGCTGTTTTAGCGCATTTTTTGTTGAAGGAGAAACTCCAGAAAATGGGAATGTTGGGGTGTCTTTTATGTGTAGTGGGTTCGACTGTGATTGTACTCCATTCGCCTGAAGAGCAATCTCTCAGTTCCGTTGAAGAAATCTGGGAATTAGCAATACAACCTGCATTTCTTTTGTATACAGCCTCGGTGGTAGCTGTAACATTAGTTCTGATGTTGTATTGTGCTCCCCGCTATGGCCAAACCAATATACTGATTTATATAGGAATCTGTTCTATAATAGGATCGTTGACAGTTATGAGTGTAAAAGCAATTGGGATTGCTATAGAACTCACAATAGAGGGCACAAACCAGCTAAAATACTTCCAAACATGGATTTTTGGAATGGTTGCAGTTACCTGTATCATCACTCAATTAAATTATCTGAACATGGCGTTGGATACTTTCAACACAGCAATTGTTTCTCCGATATATTATGCAATGTTCACATCTTTTACAATATTTGCCAGTGCAATAATGTTTAAGGATTATTCGGGTCAAAGTGTGAGCAGCATAGCATCAGAATTTTGCGGTTTCATCACTGTGTTATCTGGGACTGCTATATTGCATAGTACAAGAGAGCCAGATCCACCTTTAATTACAGATTTGTACACACCATTATCTCCGAAAGTATCATGGTACATACAAAGCAATGGGGAATGGAAGCAGAAGGATGAGGATGGCACAACCCCTAATTTCATCACTATTCTTCGGCAGGACTACTTCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mg_trans_NIPA

Protein Analysis

345

Amino Acids

37.63

Weight (kDa)

6.31

Isoelectric Point (pI)

33.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mg_trans_NIPA PF05653 5 - 295 1.1e-130 Magnesium transporter NIPA
EamA PF00892 55 - 121 6.3e-07 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 232
Acc36I ACCTGC 2 cut(s) 232, 436
AccB7I CCANNNNNTGG 1 cut(s) 313
AccI GTMKAC 1 cut(s) 443
AciI CCGC 4 cut(s) 91, 242, 496, 834
AclWI GGATC 2 cut(s) 556, 884
AcoI YGGCCR 2 cut(s) 33, 502
AcsI RAATTY 1 cut(s) 827
AcuI CTGAAG 1 cut(s) 396
AcyI GRCGYC 1 cut(s) 102
AfaI GTAC 5 cut(s) 5, 363, 877, 914, 944
AfiI CCNNNNNNNGG 2 cut(s) 313, 500
AgsI TTSAA 5 cut(s) 19, 295, 401, 712, 1030
AjnI CCWGG 1 cut(s) 231
AjuI GAANNNNNNNTTGG 2 cut(s) 683, 715
AluBI AGCT 3 cut(s) 66, 461, 619
AluI AGCT 3 cut(s) 66, 461, 619
Alw21I GWGCWC 1 cut(s) 493
Alw26I GTCTC 2 cut(s) 46, 92
AlwI GGATC 2 cut(s) 556, 884
Ama87I CYCGRG 2 cut(s) 43, 114
AoxI GGCC 5 cut(s) 33, 96, 109, 229, 502
ApeKI GCWGC 1 cut(s) 813
ApoI RAATTY 1 cut(s) 827
Asp700I GAANNNNTTC 2 cut(s) 23, 535
AspLEI GCGC 2 cut(s) 50, 283
AspS9I GGNCC 2 cut(s) 96, 110
AsuC2I CCSGG 1 cut(s) 95
AsuHPI GGTGA 1 cut(s) 229
AvaI CYCGRG 2 cut(s) 43, 114
BaeGI GKGCMC 1 cut(s) 611
BalI TGGCCA 1 cut(s) 504
Bbv12I GWGCWC 1 cut(s) 493
BbvI GCAGC 1 cut(s) 825
BccI CCATC 1 cut(s) 977
BceAI ACGGC 2 cut(s) 20, 124
BciT130I CCWGG 1 cut(s) 233
BciVI GTATCC 1 cut(s) 697
BcnI CCSGG 1 cut(s) 95
BcoDI GTCTC 2 cut(s) 46, 92
BfmI CTRYAG 1 cut(s) 588
BfoI RGCGCY 1 cut(s) 51
BfuAI ACCTGC 2 cut(s) 232, 436
BfuI GTATCC 1 cut(s) 697
BglI GCCNNNNNGGC 1 cut(s) 99
BisI GCNGC 1 cut(s) 814
BlsI GCNGC 1 cut(s) 815
Bme1390I CCNGG 2 cut(s) 95, 233
BmeT110I CYCGRG 2 cut(s) 43, 114
BmgT120I GGNCC 2 cut(s) 96, 110
BmiI GGNNCC 1 cut(s) 61
BmrFI CCNGG 2 cut(s) 95, 233
BmsI GCATC 1 cut(s) 830
BpmI CTGGAG 2 cut(s) 46, 290
BpuMI CCSGG 1 cut(s) 95
BsaHI GRCGYC 1 cut(s) 102
BsaI GGTCTC 1 cut(s) 92
BsaJI CCNNGG 1 cut(s) 450
Bsc4I CCNNNNNNNGG 2 cut(s) 313, 500
Bse118I RCCGGY 1 cut(s) 98
Bse1I ACTGG 2 cut(s) 120, 771
Bse3DI GCAATG 2 cut(s) 750, 961
BseBI CCWGG 1 cut(s) 233
BseDI CCNNGG 1 cut(s) 450
BseGI GGATG 2 cut(s) 982, 988
BseLI CCNNNNNNNGG 2 cut(s) 313, 500
BseMI GCAATG 2 cut(s) 750, 961
BseMII CTCAG 1 cut(s) 403
BseNI ACTGG 2 cut(s) 120, 771
BseSI GKGCMC 1 cut(s) 611
BseXI GCAGC 1 cut(s) 825
BshFI GGCC 5 cut(s) 35, 98, 111, 231, 504
BsiHKAI GWGCWC 1 cut(s) 493
BsiHKCI CYCGRG 2 cut(s) 43, 114
BsiSI CCGG 2 cut(s) 94, 99
BslFI GGGAC 1 cut(s) 871
BslI CCNNNNNNNGG 2 cut(s) 313, 500
BsmAI GTCTC 2 cut(s) 46, 92
BsmBI CGTCTC 1 cut(s) 46
BsmFI GGGAC 1 cut(s) 871
BsnI GGCC 5 cut(s) 35, 98, 111, 231, 504
Bso31I GGTCTC 1 cut(s) 92
BsoBI CYCGRG 2 cut(s) 43, 114
Bsp1286I GDGCHC 2 cut(s) 493, 611
Bsp1407I TGTACA 1 cut(s) 912
Bsp143I GATC 2 cut(s) 548, 889
BspACI CCGC 4 cut(s) 91, 242, 496, 834
BspANI GGCC 5 cut(s) 35, 98, 111, 231, 504
BspCNI CTCAG 1 cut(s) 402
BspLI GGNNCC 1 cut(s) 61
BspMI ACCTGC 2 cut(s) 232, 436
BspPI GGATC 2 cut(s) 556, 884
BspQI GCTCTTC 1 cut(s) 372
BspTNI GGTCTC 1 cut(s) 92
BsrDI GCAATG 2 cut(s) 750, 961
BsrFI RCCGGY 1 cut(s) 98
BsrGI TGTACA 1 cut(s) 912
BsrI ACTGG 2 cut(s) 120, 771
BssAI RCCGGY 1 cut(s) 98
BssECI CCNNGG 1 cut(s) 450
BssMI GATC 2 cut(s) 548, 889
BssNAI GTATAC 1 cut(s) 444
BssNI GRCGYC 1 cut(s) 102
Bst1107I GTATAC 1 cut(s) 444
Bst2UI CCWGG 1 cut(s) 233
Bst4CI ACNGT 3 cut(s) 355, 559, 847
Bst6I CTCTTC 1 cut(s) 372
BstACI GRCGYC 1 cut(s) 102
BstAUI TGTACA 1 cut(s) 912
BstC8I GCNNGC 2 cut(s) 100, 229
BstDEI CTNAG 1 cut(s) 389
BstEII GGTNACC 1 cut(s) 235
BstF5I GGATG 2 cut(s) 982, 988
BstH2I RGCGCY 1 cut(s) 51
BstHHI GCGC 2 cut(s) 50, 283
BstKTI GATC 2 cut(s) 551, 892
BstMAI GTCTC 2 cut(s) 46, 92
BstMBI GATC 2 cut(s) 548, 889
BstMWI GCNNNNNNNGC 4 cut(s) 99, 108, 278, 379
BstNI CCWGG 1 cut(s) 233
BstPI GGTNACC 1 cut(s) 235
BstSCI CCNGG 2 cut(s) 93, 231
BstSFI CTRYAG 1 cut(s) 588
BstSLI GKGCMC 1 cut(s) 611
BstV1I GCAGC 1 cut(s) 825
BstX2I RGATCY 1 cut(s) 889
BstYI RGATCY 1 cut(s) 889
BstZ17I GTATAC 1 cut(s) 444
BsuI GTATCC 1 cut(s) 697
BsuRI GGCC 5 cut(s) 35, 98, 111, 231, 504
BtsCI GGATG 2 cut(s) 982, 988
BtsIMutI CAGTG 3 cut(s) 127, 778, 843
BveI ACCTGC 2 cut(s) 232, 436
Cac8I GCNNGC 2 cut(s) 100, 229
CfoI GCGC 2 cut(s) 50, 283
Cfr10I RCCGGY 1 cut(s) 98
Cfr13I GGNCC 2 cut(s) 96, 110
CseI GACGC 1 cut(s) 91
Csp6I GTAC 5 cut(s) 4, 362, 876, 913, 943
CviAII CATG 3 cut(s) 636, 694, 939
CviQI GTAC 5 cut(s) 4, 362, 876, 913, 943
DdeI CTNAG 1 cut(s) 389
DpnI GATC 2 cut(s) 550, 891
DpnII GATC 2 cut(s) 548, 889
EaeI YGGCCR 2 cut(s) 33, 502
Eam1104I CTCTTC 1 cut(s) 372
EarI CTCTTC 1 cut(s) 372
EciI GGCGGA 1 cut(s) 80
Eco31I GGTCTC 1 cut(s) 92
Eco57I CTGAAG 1 cut(s) 396
Eco88I CYCGRG 2 cut(s) 43, 114
Eco91I GGTNACC 1 cut(s) 235
EcoO65I GGTNACC 1 cut(s) 235
EcoRII CCWGG 1 cut(s) 231
Esp3I CGTCTC 1 cut(s) 46
FaeI CATG 3 cut(s) 639, 697, 942
FaqI GGGAC 1 cut(s) 871
FatI CATG 3 cut(s) 635, 693, 938
FauI CCCGC 2 cut(s) 249, 503
FblI GTMKAC 1 cut(s) 443
Fnu4HI GCNGC 1 cut(s) 814
FokI GGATG 2 cut(s) 989, 995
Fsp4HI GCNGC 1 cut(s) 814
GlaI GCGC 2 cut(s) 49, 282
GluI GCNGC 1 cut(s) 814
GsuI CTGGAG 2 cut(s) 46, 290
HaeII RGCGCY 1 cut(s) 51
HaeIII GGCC 5 cut(s) 35, 98, 111, 231, 504
HapII CCGG 2 cut(s) 94, 99
HgaI GACGC 1 cut(s) 91
HhaI GCGC 2 cut(s) 50, 283
Hin1I GRCGYC 1 cut(s) 102
Hin1II CATG 3 cut(s) 639, 697, 942
Hin6I GCGC 2 cut(s) 48, 281
HinP1I GCGC 2 cut(s) 48, 281
HincII GTYRAC 2 cut(s) 106, 555
HindII GTYRAC 2 cut(s) 106, 555
HinfI GANTC 1 cut(s) 531
HpaII CCGG 2 cut(s) 94, 99
HphI GGTGA 1 cut(s) 229
Hpy166II GTNNAC 5 cut(s) 106, 444, 555, 750, 915
Hpy188I TCNGA 5 cut(s) 477, 690, 732, 826, 930
Hpy188III TCNNGA 3 cut(s) 43, 76, 307
Hpy8I GTNNAC 5 cut(s) 106, 444, 555, 750, 915
Hpy99I CGWCG 1 cut(s) 41
HpyAV CCTTC 4 cut(s) 61, 76, 289, 967
HpyCH4III ACNGT 3 cut(s) 355, 559, 847
HpyCH4V TGCA 6 cut(s) 251, 431, 656, 743, 776, 871
HpyF10VI GCNNNNNNNGC 4 cut(s) 99, 108, 278, 379
HpyF3I CTNAG 1 cut(s) 389
Hsp92I GRCGYC 1 cut(s) 102
Hsp92II CATG 3 cut(s) 639, 697, 942
HspAI GCGC 2 cut(s) 48, 281
KroI GCCGGC 1 cut(s) 98
KroNI GCCGGC 1 cut(s) 100
Kzo9I GATC 2 cut(s) 548, 889
LguI GCTCTTC 1 cut(s) 372
LmnI GCTCC 2 cut(s) 65, 496
Lsp1109I GCAGC 1 cut(s) 825
LweI GCATC 1 cut(s) 830
MaeIII GTNAC 4 cut(s) 172, 235, 463, 658
MalI GATC 2 cut(s) 550, 891
MboI GATC 2 cut(s) 548, 889
MboII GAAGA 4 cut(s) 91, 389, 413, 1004
MfeI CAATTG 2 cut(s) 576, 720
MflI RGATCY 1 cut(s) 889
MhlI GDGCHC 2 cut(s) 493, 611
MlsI TGGCCA 1 cut(s) 504
MluNI TGGCCA 1 cut(s) 504
MmeI TCCRAC 2 cut(s) 166, 681
MnlI CCTC 6 cut(s) 122, 123, 162, 460, 598, 973
Mox20I TGGCCA 1 cut(s) 504
MroNI GCCGGC 1 cut(s) 98
MroXI GAANNNNTTC 2 cut(s) 23, 535
MscI TGGCCA 1 cut(s) 504
MseI TTAA 3 cut(s) 680, 786, 899
MslI CAYNNNNRTG 1 cut(s) 845
Msp20I TGGCCA 1 cut(s) 504
MspI CCGG 2 cut(s) 94, 99
MspR9I CCNGG 2 cut(s) 95, 233
MunI CAATTG 2 cut(s) 576, 720
MvaI CCWGG 1 cut(s) 233
MwoI GCNNNNNNNGC 4 cut(s) 99, 108, 278, 379
NaeI GCCGGC 1 cut(s) 100
NciI CCSGG 1 cut(s) 95
NdeII GATC 2 cut(s) 548, 889
NgoMIV GCCGGC 1 cut(s) 98
NlaIII CATG 3 cut(s) 639, 697, 942
NlaIV GGNNCC 1 cut(s) 61
NmuCI GTSAC 1 cut(s) 235
PaeR7I CTCGAG 2 cut(s) 43, 114
PaqCI CACCTGC 1 cut(s) 232
PciSI GCTCTTC 1 cut(s) 372
PdiI GCCGGC 1 cut(s) 100
PdmI GAANNNNTTC 2 cut(s) 23, 535
PfeI GAWTC 1 cut(s) 531
PflMI CCANNNNNTGG 1 cut(s) 313
PkrI GCNGC 1 cut(s) 815
Psp6I CCWGG 1 cut(s) 231
PspEI GGTNACC 1 cut(s) 235
PspGI CCWGG 1 cut(s) 231
PspN4I GGNNCC 1 cut(s) 61
PspPI GGNCC 2 cut(s) 96, 110
PspXI VCTCGAGB 1 cut(s) 114
PsrI GAACNNNNNNTAC 2 cut(s) 456, 488
PsuI RGATCY 1 cut(s) 889
RsaI GTAC 5 cut(s) 5, 363, 877, 914, 944
RsaNI GTAC 5 cut(s) 4, 362, 876, 913, 943
RseI CAYNNNNRTG 1 cut(s) 845
SapI GCTCTTC 1 cut(s) 372
SaqAI TTAA 3 cut(s) 680, 786, 899
SatI GCNGC 1 cut(s) 814
Sau3AI GATC 2 cut(s) 548, 889
Sau96I GGNCC 2 cut(s) 96, 110
ScrFI CCNGG 2 cut(s) 95, 233
SduI GDGCHC 2 cut(s) 493, 611
SetI ASST 7 cut(s) 68, 226, 430, 463, 621, 665, 898
SfaNI GCATC 1 cut(s) 830
SfcI CTRYAG 1 cut(s) 588
Sfr274I CTCGAG 2 cut(s) 43, 114
SlaI CTCGAG 2 cut(s) 43, 114
SmiMI CAYNNNNRTG 1 cut(s) 845
SmlI CTYRAG 3 cut(s) 7, 43, 114
SmoI CTYRAG 3 cut(s) 7, 43, 114
SsiI CCGC 4 cut(s) 91, 242, 496, 834
SspI AATATT 1 cut(s) 765
StyD4I CCNGG 2 cut(s) 93, 231
TaaI ACNGT 3 cut(s) 355, 559, 847
TaqI TCGA 3 cut(s) 44, 115, 350
TatI WGTACW 4 cut(s) 3, 361, 875, 912
TfiI GAWTC 1 cut(s) 531
Tru1I TTAA 3 cut(s) 680, 786, 899
Tru9I TTAA 3 cut(s) 680, 786, 899
TscAI CASTG 3 cut(s) 127, 778, 850
TseFI GTSAC 1 cut(s) 235
TseI GCWGC 1 cut(s) 813
Tsp45I GTSAC 1 cut(s) 235
TspDTI ATGAA 5 cut(s) 16, 43, 58, 829, 991
TspGWI ACGGA 1 cut(s) 385
TspRI CASTG 3 cut(s) 127, 778, 850
Van91I CCANNNNNTGG 1 cut(s) 313
XapI RAATTY 1 cut(s) 827
XhoI CTCGAG 2 cut(s) 43, 114
XmiI GTMKAC 1 cut(s) 443
XmnI GAANNNNTTC 2 cut(s) 23, 535
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.