Acts as a Mg(2 ) transporter. Can also transport other divalent cations such as Fe(2 ), Sr(2 ), Ba(2 ), Mn(2 ) and Co(2 ) but to a much less extent than Mg(2 )
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 347 |
| AccB1I | GGYRCC | 1 cut(s) | 113 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 503 |
| AccI | GTMKAC | 1 cut(s) | 525 |
| AccII | CGCG | 2 cut(s) | 65, 99 |
| AccIII | TCCGGA | 1 cut(s) | 10 |
| AciI | CCGC | 1 cut(s) | 108 |
| AclI | AACGTT | 1 cut(s) | 970 |
| AclWI | GGATC | 2 cut(s) | 358, 361 |
| AcsI | RAATTY | 2 cut(s) | 394, 988 |
| AcuI | CTGAAG | 1 cut(s) | 981 |
| AdeI | CACNNNGTG | 1 cut(s) | 251 |
| AfaI | GTAC | 1 cut(s) | 932 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 503 |
| AgsI | TTSAA | 4 cut(s) | 298, 494, 715, 790 |
| AhlI | ACTAGT | 1 cut(s) | 520 |
| AjiI | CACGTC | 1 cut(s) | 639 |
| AjnI | CCWGG | 2 cut(s) | 417, 465 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 420, 452 |
| AleI | CACNNNNGTG | 1 cut(s) | 160 |
| AluBI | AGCT | 3 cut(s) | 69, 455, 598 |
| AluI | AGCT | 3 cut(s) | 69, 455, 598 |
| Alw21I | GWGCWC | 1 cut(s) | 256 |
| Alw26I | GTCTC | 1 cut(s) | 95 |
| Alw44I | GTGCAC | 1 cut(s) | 252 |
| AlwI | GGATC | 2 cut(s) | 358, 361 |
| Ama87I | CYCGRG | 1 cut(s) | 117 |
| Aor13HI | TCCGGA | 1 cut(s) | 10 |
| AoxI | GGCC | 2 cut(s) | 105, 210 |
| ApaLI | GTGCAC | 1 cut(s) | 252 |
| ApeKI | GCWGC | 2 cut(s) | 719, 816 |
| ApoI | RAATTY | 2 cut(s) | 394, 988 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 538 |
| AspLEI | GCGC | 3 cut(s) | 65, 99, 101 |
| AspS9I | GGNCC | 2 cut(s) | 105, 415 |
| AsuC2I | CCSGG | 1 cut(s) | 103 |
| AvaI | CYCGRG | 1 cut(s) | 117 |
| AvaII | GGWCC | 1 cut(s) | 415 |
| AxyI | CCTNAGG | 1 cut(s) | 378 |
| BaeGI | GKGCMC | 1 cut(s) | 256 |
| BanI | GGYRCC | 1 cut(s) | 113 |
| Bbv12I | GWGCWC | 1 cut(s) | 256 |
| BbvI | GCAGC | 2 cut(s) | 731, 828 |
| BccI | CCATC | 4 cut(s) | 34, 541, 872, 904 |
| BciT130I | CCWGG | 2 cut(s) | 419, 467 |
| BciVI | GTATCC | 1 cut(s) | 700 |
| BclI | TGATCA | 1 cut(s) | 174 |
| BcnI | CCSGG | 1 cut(s) | 103 |
| BcoDI | GTCTC | 1 cut(s) | 95 |
| BcuI | ACTAGT | 1 cut(s) | 520 |
| BfaI | CTAG | 2 cut(s) | 521, 774 |
| BfmI | CTRYAG | 1 cut(s) | 399 |
| BfuAI | ACCTGC | 1 cut(s) | 347 |
| BfuI | GTATCC | 1 cut(s) | 700 |
| BisI | GCNGC | 3 cut(s) | 108, 720, 817 |
| BlsI | GCNGC | 3 cut(s) | 109, 721, 818 |
| Bme1390I | CCNGG | 3 cut(s) | 103, 419, 467 |
| Bme18I | GGWCC | 1 cut(s) | 415 |
| BmeT110I | CYCGRG | 1 cut(s) | 117 |
| BmgBI | CACGTC | 1 cut(s) | 639 |
| BmgT120I | GGNCC | 2 cut(s) | 105, 415 |
| BmiI | GGNNCC | 6 cut(s) | 9, 106, 115, 416, 862, 894 |
| BmrFI | CCNGG | 3 cut(s) | 103, 419, 467 |
| BmrI | ACTGGG | 1 cut(s) | 728 |
| BmuI | ACTGGG | 1 cut(s) | 728 |
| BpuEI | CTTGAG | 2 cut(s) | 77, 626 |
| BpuMI | CCSGG | 1 cut(s) | 103 |
| BsaBI | GATNNNNATC | 1 cut(s) | 365 |
| BsaI | GGTCTC | 1 cut(s) | 95 |
| BsaJI | CCNNGG | 2 cut(s) | 102, 465 |
| BsaWI | WCCGGW | 1 cut(s) | 10 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 933, 963 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 503 |
| Bse118I | RCCGGY | 1 cut(s) | 110 |
| Bse1I | ACTGG | 2 cut(s) | 39, 734 |
| Bse21I | CCTNAGG | 1 cut(s) | 378 |
| Bse8I | GATNNNNATC | 1 cut(s) | 365 |
| BseAI | TCCGGA | 1 cut(s) | 10 |
| BseBI | CCWGG | 2 cut(s) | 419, 467 |
| BseDI | CCNNGG | 2 cut(s) | 102, 465 |
| BseGI | GGATG | 2 cut(s) | 72, 335 |
| BseJI | GATNNNNATC | 1 cut(s) | 365 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 503 |
| BseMII | CTCAG | 3 cut(s) | 248, 392, 647 |
| BseNI | ACTGG | 2 cut(s) | 39, 734 |
| BsePI | GCGCGC | 1 cut(s) | 97 |
| BseRI | GAGGAG | 1 cut(s) | 954 |
| BseSI | GKGCMC | 1 cut(s) | 256 |
| BseXI | GCAGC | 2 cut(s) | 731, 828 |
| Bsh1236I | CGCG | 2 cut(s) | 65, 99 |
| BshFI | GGCC | 2 cut(s) | 107, 212 |
| BshNI | GGYRCC | 1 cut(s) | 113 |
| BsiHKAI | GWGCWC | 1 cut(s) | 256 |
| BsiHKCI | CYCGRG | 1 cut(s) | 117 |
| BsiSI | CCGG | 4 cut(s) | 11, 102, 111, 227 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 503 |
| BsmAI | GTCTC | 1 cut(s) | 95 |
| BsnI | GGCC | 2 cut(s) | 107, 212 |
| Bso31I | GGTCTC | 1 cut(s) | 95 |
| BsoBI | CYCGRG | 1 cut(s) | 117 |
| Bsp1286I | GDGCHC | 1 cut(s) | 256 |
| Bsp13I | TCCGGA | 1 cut(s) | 10 |
| Bsp143I | GATC | 5 cut(s) | 174, 350, 360, 366, 886 |
| BspACI | CCGC | 1 cut(s) | 108 |
| BspANI | GGCC | 2 cut(s) | 107, 212 |
| BspCNI | CTCAG | 3 cut(s) | 249, 391, 646 |
| BspEI | TCCGGA | 1 cut(s) | 10 |
| BspFNI | CGCG | 2 cut(s) | 65, 99 |
| BspLI | GGNNCC | 6 cut(s) | 9, 106, 115, 416, 862, 894 |
| BspMI | ACCTGC | 1 cut(s) | 347 |
| BspPI | GGATC | 2 cut(s) | 358, 361 |
| BspT107I | GGYRCC | 1 cut(s) | 113 |
| BspTNI | GGTCTC | 1 cut(s) | 95 |
| BsrFI | RCCGGY | 1 cut(s) | 110 |
| BsrI | ACTGG | 2 cut(s) | 39, 734 |
| BssAI | RCCGGY | 1 cut(s) | 110 |
| BssECI | CCNNGG | 2 cut(s) | 102, 465 |
| BssHII | GCGCGC | 1 cut(s) | 97 |
| BssMI | GATC | 5 cut(s) | 174, 350, 360, 366, 886 |
| Bst2UI | CCWGG | 2 cut(s) | 419, 467 |
| Bst4CI | ACNGT | 6 cut(s) | 562, 655, 681, 850, 922, 1008 |
| Bst6I | CTCTTC | 1 cut(s) | 947 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 728 |
| BstC8I | GCNNGC | 4 cut(s) | 67, 99, 112, 169 |
| BstDEI | CTNAG | 3 cut(s) | 257, 378, 633 |
| BstF5I | GGATG | 2 cut(s) | 72, 335 |
| BstFNI | CGCG | 2 cut(s) | 65, 99 |
| BstHHI | GCGC | 3 cut(s) | 65, 99, 101 |
| BstKTI | GATC | 5 cut(s) | 177, 353, 363, 369, 889 |
| BstMAI | GTCTC | 1 cut(s) | 95 |
| BstMBI | GATC | 5 cut(s) | 174, 350, 360, 366, 886 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 107, 281, 728 |
| BstNI | CCWGG | 2 cut(s) | 419, 467 |
| BstSCI | CCNGG | 3 cut(s) | 101, 417, 465 |
| BstSFI | CTRYAG | 1 cut(s) | 399 |
| BstSLI | GKGCMC | 1 cut(s) | 256 |
| BstUI | CGCG | 2 cut(s) | 65, 99 |
| BstV1I | GCAGC | 2 cut(s) | 731, 828 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 162, 208 |
| Bsu36I | CCTNAGG | 1 cut(s) | 378 |
| BsuI | GTATCC | 1 cut(s) | 700 |
| BsuRI | GGCC | 2 cut(s) | 107, 212 |
| BtgZI | GCGATG | 1 cut(s) | 53 |
| BtrI | CACGTC | 1 cut(s) | 639 |
| BtsCI | GGATG | 2 cut(s) | 72, 335 |
| BtsIMutI | CAGTG | 3 cut(s) | 254, 277, 954 |
| BveI | ACCTGC | 1 cut(s) | 347 |
| Cac8I | GCNNGC | 4 cut(s) | 67, 99, 112, 169 |
| CfoI | GCGC | 3 cut(s) | 65, 99, 101 |
| Cfr10I | RCCGGY | 1 cut(s) | 110 |
| Cfr13I | GGNCC | 2 cut(s) | 105, 415 |
| Csp6I | GTAC | 1 cut(s) | 931 |
| CviAII | CATG | 4 cut(s) | 172, 371, 875, 927 |
| CviQI | GTAC | 1 cut(s) | 931 |
| DdeI | CTNAG | 3 cut(s) | 257, 378, 633 |
| DpnI | GATC | 5 cut(s) | 176, 352, 362, 368, 888 |
| DpnII | GATC | 5 cut(s) | 174, 350, 360, 366, 886 |
| DraIII | CACNNNGTG | 1 cut(s) | 251 |
| Eam1104I | CTCTTC | 1 cut(s) | 947 |
| EarI | CTCTTC | 1 cut(s) | 947 |
| Eco31I | GGTCTC | 1 cut(s) | 95 |
| Eco47I | GGWCC | 1 cut(s) | 415 |
| Eco57I | CTGAAG | 1 cut(s) | 981 |
| Eco81I | CCTNAGG | 1 cut(s) | 378 |
| Eco88I | CYCGRG | 1 cut(s) | 117 |
| EcoO109I | RGGNCCY | 1 cut(s) | 415 |
| EcoRI | GAATTC | 1 cut(s) | 988 |
| EcoRII | CCWGG | 2 cut(s) | 417, 465 |
| FaeI | CATG | 4 cut(s) | 175, 374, 878, 930 |
| FatI | CATG | 4 cut(s) | 171, 370, 874, 926 |
| FbaI | TGATCA | 1 cut(s) | 174 |
| FblI | GTMKAC | 1 cut(s) | 525 |
| Fnu4HI | GCNGC | 3 cut(s) | 108, 720, 817 |
| FokI | GGATG | 2 cut(s) | 59, 342 |
| Fsp4HI | GCNGC | 3 cut(s) | 108, 720, 817 |
| FspBI | CTAG | 2 cut(s) | 521, 774 |
| GlaI | GCGC | 3 cut(s) | 64, 98, 100 |
| GluI | GCNGC | 3 cut(s) | 108, 720, 817 |
| HaeIII | GGCC | 2 cut(s) | 107, 212 |
| HapII | CCGG | 4 cut(s) | 11, 102, 111, 227 |
| HhaI | GCGC | 3 cut(s) | 65, 99, 101 |
| Hin1II | CATG | 4 cut(s) | 175, 374, 878, 930 |
| Hin6I | GCGC | 3 cut(s) | 63, 97, 99 |
| HinP1I | GCGC | 3 cut(s) | 63, 97, 99 |
| HinfI | GANTC | 4 cut(s) | 514, 534, 840, 944 |
| HpaII | CCGG | 4 cut(s) | 11, 102, 111, 227 |
| Hpy166II | GTNNAC | 4 cut(s) | 254, 526, 554, 753 |
| Hpy188I | TCNGA | 8 cut(s) | 621, 636, 651, 762, 807, 829, 961, 994 |
| Hpy188III | TCNNGA | 9 cut(s) | 11, 76, 358, 364, 380, 605, 858, 890, 1001 |
| Hpy8I | GTNNAC | 4 cut(s) | 254, 526, 554, 753 |
| HpyAV | CCTTC | 3 cut(s) | 16, 79, 292 |
| HpyCH4III | ACNGT | 6 cut(s) | 562, 655, 681, 850, 922, 1008 |
| HpyCH4IV | ACGT | 2 cut(s) | 638, 970 |
| HpyCH4V | TGCA | 7 cut(s) | 254, 342, 374, 487, 590, 719, 878 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 107, 281, 728 |
| HpyF3I | CTNAG | 3 cut(s) | 257, 378, 633 |
| HpySE526I | ACGT | 2 cut(s) | 638, 970 |
| Hsp92II | CATG | 4 cut(s) | 175, 374, 878, 930 |
| HspAI | GCGC | 3 cut(s) | 63, 97, 99 |
| Kpn2I | TCCGGA | 1 cut(s) | 10 |
| KroI | GCCGGC | 1 cut(s) | 110 |
| KroNI | GCCGGC | 1 cut(s) | 112 |
| Ksp22I | TGATCA | 1 cut(s) | 174 |
| Kzo9I | GATC | 5 cut(s) | 174, 350, 360, 366, 886 |
| LmnI | GCTCC | 2 cut(s) | 229, 907 |
| Lsp1109I | GCAGC | 2 cut(s) | 731, 828 |
| MaeI | CTAG | 2 cut(s) | 521, 774 |
| MaeII | ACGT | 2 cut(s) | 638, 970 |
| MaeIII | GTNAC | 4 cut(s) | 238, 334, 673, 922 |
| MalI | GATC | 5 cut(s) | 176, 352, 362, 368, 888 |
| MboI | GATC | 5 cut(s) | 174, 350, 360, 366, 886 |
| MboII | GAAGA | 2 cut(s) | 964, 974 |
| MfeI | CAATTG | 2 cut(s) | 579, 723 |
| MhlI | GDGCHC | 1 cut(s) | 256 |
| MnlI | CCTC | 6 cut(s) | 387, 397, 601, 642, 835, 932 |
| MroI | TCCGGA | 1 cut(s) | 10 |
| MroNI | GCCGGC | 1 cut(s) | 110 |
| MroXI | GAANNNNTTC | 1 cut(s) | 538 |
| MseI | TTAA | 2 cut(s) | 683, 693 |
| MslI | CAYNNNNRTG | 2 cut(s) | 160, 848 |
| MspI | CCGG | 4 cut(s) | 11, 102, 111, 227 |
| MspR9I | CCNGG | 3 cut(s) | 103, 419, 467 |
| MunI | CAATTG | 2 cut(s) | 579, 723 |
| MvaI | CCWGG | 2 cut(s) | 419, 467 |
| MvnI | CGCG | 2 cut(s) | 65, 99 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 107, 281, 728 |
| NaeI | GCCGGC | 1 cut(s) | 112 |
| NciI | CCSGG | 1 cut(s) | 103 |
| NdeII | GATC | 5 cut(s) | 174, 350, 360, 366, 886 |
| NgoMIV | GCCGGC | 1 cut(s) | 110 |
| NlaIII | CATG | 4 cut(s) | 175, 374, 878, 930 |
| NlaIV | GGNNCC | 6 cut(s) | 9, 106, 115, 416, 862, 894 |
| OliI | CACNNNNGTG | 1 cut(s) | 160 |
| PauI | GCGCGC | 1 cut(s) | 97 |
| PdiI | GCCGGC | 1 cut(s) | 112 |
| PdmI | GAANNNNTTC | 1 cut(s) | 538 |
| PfeI | GAWTC | 4 cut(s) | 514, 534, 840, 944 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 503 |
| PkrI | GCNGC | 3 cut(s) | 109, 721, 818 |
| PpuMI | RGGWCCY | 1 cut(s) | 415 |
| Psp1406I | AACGTT | 1 cut(s) | 970 |
| Psp5II | RGGWCCY | 1 cut(s) | 415 |
| Psp6I | CCWGG | 2 cut(s) | 417, 465 |
| PspGI | CCWGG | 2 cut(s) | 417, 465 |
| PspN4I | GGNNCC | 6 cut(s) | 9, 106, 115, 416, 862, 894 |
| PspPI | GGNCC | 2 cut(s) | 105, 415 |
| PspPPI | RGGWCCY | 1 cut(s) | 415 |
| PteI | GCGCGC | 1 cut(s) | 97 |
| RsaI | GTAC | 1 cut(s) | 932 |
| RsaNI | GTAC | 1 cut(s) | 931 |
| RseI | CAYNNNNRTG | 2 cut(s) | 160, 848 |
| SaqAI | TTAA | 2 cut(s) | 683, 693 |
| SatI | GCNGC | 3 cut(s) | 108, 720, 817 |
| Sau3AI | GATC | 5 cut(s) | 174, 350, 360, 366, 886 |
| Sau96I | GGNCC | 2 cut(s) | 105, 415 |
| ScrFI | CCNGG | 3 cut(s) | 103, 419, 467 |
| SduI | GDGCHC | 1 cut(s) | 256 |
| SetI | ASST | 7 cut(s) | 71, 341, 379, 457, 600, 641, 973 |
| SfcI | CTRYAG | 1 cut(s) | 399 |
| SinI | GGWCC | 1 cut(s) | 415 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 160, 848 |
| SmlI | CTYRAG | 2 cut(s) | 92, 605 |
| SmoI | CTYRAG | 2 cut(s) | 92, 605 |
| SpeI | ACTAGT | 1 cut(s) | 520 |
| SsiI | CCGC | 1 cut(s) | 108 |
| SspMI | CTAG | 2 cut(s) | 521, 774 |
| StyD4I | CCNGG | 3 cut(s) | 101, 417, 465 |
| TaaI | ACNGT | 6 cut(s) | 562, 655, 681, 850, 922, 1008 |
| TaiI | ACGT | 2 cut(s) | 641, 973 |
| TaqI | TCGA | 1 cut(s) | 47 |
| TauI | GCSGC | 1 cut(s) | 110 |
| TfiI | GAWTC | 4 cut(s) | 514, 534, 840, 944 |
| Tru1I | TTAA | 2 cut(s) | 683, 693 |
| Tru9I | TTAA | 2 cut(s) | 683, 693 |
| TscAI | CASTG | 3 cut(s) | 261, 277, 954 |
| TseI | GCWGC | 2 cut(s) | 719, 816 |
| TspDTI | ATGAA | 4 cut(s) | 46, 61, 832, 981 |
| TspRI | CASTG | 3 cut(s) | 261, 277, 954 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 503 |
| VneI | GTGCAC | 1 cut(s) | 252 |
| VpaK11BI | GGWCC | 1 cut(s) | 415 |
| XapI | RAATTY | 2 cut(s) | 394, 988 |
| XmiI | GTMKAC | 1 cut(s) | 525 |
| XmnI | GAANNNNTTC | 1 cut(s) | 538 |
| XspI | CTAG | 2 cut(s) | 521, 774 |