Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0004158

Physical Location & Seq

Genomic Coordinates Reverse (-)

25924164 .. 25929514

Gene Structure 5351 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1188900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1755 bp

ATGGACGCGACCGCACTCGACCAACCTTCCGGACCCTCCGATTACGTCGATCCGGGTGATTTCCACCCCAGCTCCGAGCCGGCCTTTTACTCTTCTGCTGCTGCTTCCGGTGGGGCCAAGTACAAGCTCATGTCCCCGGCCAAGCTTCCGATCTCGAGGTCCCCCTGCCTCACTATCCCTCCCGGCCTCAGTCCGACGTCATTTCTCGAGTCCCCTGTCCTCCTCTCCAACATGAAGGCAGAACCTTCCCCAACTACTGGGTCCTTTTTGAAGCCTCAAATGTTGTACGGATCTCTGAGTTCTACTACATACTCAGCAACCACAGTTTGCTCGGATTTCAATACCTCGGATGAAAGAAATTCTGGAAGCTTCGAGTTTAAGCCCCATGCTAGATCAAATGTGGTTACTACAGATTATAACCACTATAGGAATGAGCAGCCTGTGCAAGTCCCAGGTCAAGCTGAGCCTCAATCACATGCATCACCAACTTTGGCTAAAAATGAGATGGCAGTCTCTTCAAATGAATTGAGTTTATCTGCACCTGTTCACACTGGAGGAGCTAGTGCACCCGTGGAAGATGATTCGGGTGACTTAACACAGAGGGAACATCCACATCCTGGGGTCCAAACATCTCAGGGTGATAACAAAGGAAATGGGCCTTCAGTAATATCCTCTGATGATGGGTATAACTGGCGAAAATATGGACAAAAGCATGTTAAAGGAAGTGAATTTCCTCGCAGTTATTACAAATGTACCCATCCTAATTGTGAAGTGAAGAAGCTATTTGAGCGATCTCATGATGGACAGATAACAGAGATTATCTACAAGGGTACACATGATCATCCTAAGCCTCAACCAAGTCGACGATATAATACTGGTGCTATGATGCCTGCTCAAGAAGAAAGATCTGACAAGGCTTCATCATTGACTGGTCAAGATGACAAACCATCCAGCATTTATGGGCAAATGTCTAGTAACAATGAGCCAAACAGTACTCCTGAGCTCTCTCCTGTCACAGGCAATGATGATAGTGTAGAAGGCACAGGCTCACTGTCAAATAGGTTGCCAGATGAGGTTGATGAGGATGACCAATTCTCAAAAAGGAGGAGGATGGATGTCGGTAGCATTGATGTCACACCGATTGTTAAGCCTATCCGAGAACCACGAGTTGTTGTTCAGACTCTCAGTGAGGTTGATATATTAGATGATGGATACCGCTGGCGCAAATATGGTCAGAAAGTGGTGAGGGGAAATCCTAATCCAAGGAGCTATTACAAGTGCACCAATGCTGGATGCCCTGTTAGAAAACATGTGGAGAGGGCATCGCATGATCCAAAAGCAGTTATAACTACATATGAAGGGAAACATAATCATGACGTCCCTACTGCAAAGTCTAGTAGCCATGACACTTCAGGACCAACAACTGTGAATGTCCCGTCGAGAATTAGACCAGAAGAAAGTGACACCATAAGCCTTGATCTTGGTGTTGGAATGACCTCTGGTGGCGAAATTAGAACCAACGAGCACGTGCAACTGCACTCTGATCTTGTGGAACGGCAATCTCACACTAATTCCAATTTCAGGTTTGCTCAAAATACCCCTGTTTCGACATACTATGGTGTTCTTAATGGTGGCATGAATCAGTATGGATCTAGGGAAAACCCAAGTGAATCACGCAGCATGGAAATTCTACCTTTGAATCATTCCTCGTACCCATATCCACAGAACATGGGAAGAGTACTAACAGGTCCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0006355 GO:0006950 GO:0008150 GO:0009611 GO:0009889 GO:0009891 GO:0009893 GO:0009961 GO:0010033 GO:0010243 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0042221 GO:0043200 GO:0043565 GO:0045893 GO:0045935 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1901698 GO:1901700 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WRKY

Protein Analysis

585

Amino Acids

63.85

Weight (kDa)

5.97

Isoelectric Point (pI)

49.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WRKY	PF03106	226 - 282	7.9e-26	WRKY DNA -binding domain
WRKY	PF03106	402 - 459	1.2e-26	WRKY DNA -binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004158)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G26640 AT4G26640 AT4G26640 AT4G26640 AT4G26640 AT4G26640 AT4G26640
fragaria_vesca	FvH4_3g24010 FvH4_3g24010
malus_domestica	MD03G1188900.v1.1 MD11G1205000.v1.1
prunus_persica	Prupe.4G232600_v2.0.a1 Prupe.4G232600_v2.0.a1 Prupe.4G232600_v2.0.a1
pyrus_communis	pycom03g14170 pycom11g17770
rosa_chinensis	RchiOBHm_Chr5g0042581 RchiOBHm_Chr5g0042601
rosa_laevigata	RLG00000034142
rosa_multiflora	Rmu_sc0004704.1_g000005 Rmu_sc0004704.1_g000010 Rmu_sc0012301.1_g000004 Rmu_sc0012301.1_g000009
rosa_roxburghii	Rroxscaffold_1G00038060
rosa_rugosa	Rorug05G0202900 Rorug05G0203000 Rorug05G0203100
rosa_samantha	Rh5AG287900 Rh5AG288300 Rh5BG292300 Rh5CG324700 Rh5DG302100
rosa_wichuraiana	Rw5G026730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	417, 1346
AatII	GACGTC	2 cut(s)	200, 1380
AccB7I	CCANNNNNTGG	2 cut(s)	257, 617
AccI	GTMKAC	1 cut(s)	862
AccII	CGCG	1 cut(s)	8
AccIII	TCCGGA	1 cut(s)	29
AciI	CCGC	2 cut(s)	12, 1216
AclWI	GGATC	4 cut(s)	44, 298, 1325, 1657
AcoI	YGGCCR	1 cut(s)	138
AcsI	RAATTY	3 cut(s)	358, 728, 1686
AcuI	CTGAAG	2 cut(s)	645, 1395
AcvI	CACGTG	1 cut(s)	1528
AcyI	GRCGYC	2 cut(s)	197, 1377
AfaI	GTAC	7 cut(s)	122, 287, 754, 832, 994, 1712, 1740
AfiI	CCNNNNNNNGG	4 cut(s)	257, 617, 1016, 1581
AflIII	ACRYGT	1 cut(s)	1309
AgsI	TTSAA	4 cut(s)	271, 340, 519, 1699
AjnI	CCWGG	2 cut(s)	451, 616
AluBI	AGCT	9 cut(s)	72, 127, 145, 369, 461, 560, 781, 1003, 1269
AluI	AGCT	9 cut(s)	72, 127, 145, 369, 461, 560, 781, 1003, 1269
Alw21I	GWGCWC	4 cut(s)	568, 1005, 1283, 1527
Alw26I	GTCTC	1 cut(s)	517
Alw44I	GTGCAC	2 cut(s)	564, 1279
AlwI	GGATC	4 cut(s)	44, 298, 1325, 1657
Ama87I	CYCGRG	2 cut(s)	154, 206
Aor13HI	TCCGGA	1 cut(s)	29
AoxI	GGCC	5 cut(s)	81, 114, 138, 184, 656
ApaLI	GTGCAC	2 cut(s)	564, 1279
ApeKI	GCWGC	4 cut(s)	98, 101, 436, 1677
ApoI	RAATTY	3 cut(s)	358, 728, 1686
AspLEI	GCGC	1 cut(s)	1224
AspS9I	GGNCC	8 cut(s)	32, 114, 159, 261, 622, 656, 1415, 1748
AsuC2I	CCSGG	3 cut(s)	54, 137, 183
AsuHPI	GGTGA	5 cut(s)	68, 474, 599, 650, 1255
AvaI	CYCGRG	2 cut(s)	154, 206
AvaII	GGWCC	6 cut(s)	32, 159, 261, 622, 1415, 1748
BaeGI	GKGCMC	2 cut(s)	568, 1283
BaeI	ACNNNNGTAYC	2 cut(s)	736, 769
BanII	GRGCYC	1 cut(s)	1005
BauI	CACGAG	1 cut(s)	1164
BbrPI	CACGTG	1 cut(s)	1528
Bbv12I	GWGCWC	4 cut(s)	568, 1005, 1283, 1527
BbvI	GCAGC	4 cut(s)	85, 88, 448, 1689
BccI	CCATC	7 cut(s)	499, 674, 765, 794, 955, 1105, 1202
BceAI	ACGGC	1 cut(s)	1571
BciT130I	CCWGG	2 cut(s)	453, 618
BciVI	GTATCC	1 cut(s)	1205
BclI	TGATCA	1 cut(s)	838
BcnI	CCSGG	3 cut(s)	54, 137, 183
BcoDI	GTCTC	1 cut(s)	517
BfaI	CTAG	5 cut(s)	390, 561, 972, 1395, 1653
BfmI	CTRYAG	2 cut(s)	408, 424
BfuI	GTATCC	1 cut(s)	1205
BglII	AGATCT	1 cut(s)	905
BisI	GCNGC	4 cut(s)	99, 102, 437, 1678
BlpI	GCTNAGC	1 cut(s)	462
BlsI	GCNGC	4 cut(s)	100, 103, 438, 1679
BmcAI	AGTACT	2 cut(s)	994, 1740
Bme1390I	CCNGG	5 cut(s)	54, 137, 183, 453, 618
Bme18I	GGWCC	6 cut(s)	32, 159, 261, 622, 1415, 1748
BmeT110I	CYCGRG	2 cut(s)	154, 206
BmgT120I	GGNCC	8 cut(s)	32, 114, 159, 261, 622, 656, 1415, 1748
BmiI	GGNNCC	5 cut(s)	34, 115, 161, 262, 623
BmrFI	CCNGG	5 cut(s)	54, 137, 183, 453, 618
BmrI	ACTGGG	1 cut(s)	267
BmsI	GCATC	4 cut(s)	488, 876, 1283, 1331
BmuI	ACTGGG	1 cut(s)	267
BpmI	CTGGAG	1 cut(s)	573
Bpu10I	CCTNAGC	2 cut(s)	846, 999
Bpu1102I	GCTNAGC	1 cut(s)	462
BpuEI	CTTGAG	1 cut(s)	879
BpuMI	CCSGG	3 cut(s)	54, 137, 183
BsaAI	YACGTR	1 cut(s)	1528
BsaHI	GRCGYC	2 cut(s)	197, 1377
BsaJI	CCNNGG	6 cut(s)	135, 345, 451, 570, 617, 1262
BsaWI	WCCGGW	2 cut(s)	29, 107
BsaXI	ACNNNNNCTCC	4 cut(s)	56, 86, 163, 193
Bsc4I	CCNNNNNNNGG	4 cut(s)	257, 617, 1016, 1581
Bse118I	RCCGGY	1 cut(s)	79
Bse1I	ACTGG	5 cut(s)	262, 556, 695, 880, 934
Bse3DI	GCAATG	1 cut(s)	1027
BseAI	TCCGGA	1 cut(s)	29
BseBI	CCWGG	2 cut(s)	453, 618
BseDI	CCNNGG	6 cut(s)	135, 345, 451, 570, 617, 1262
BseLI	CCNNNNNNNGG	4 cut(s)	257, 617, 1016, 1581
BseMI	GCAATG	1 cut(s)	1027
BseMII	CTCAG	7 cut(s)	202, 287, 327, 453, 647, 990, 1198
BseNI	ACTGG	5 cut(s)	262, 556, 695, 880, 934
BseRI	GAGGAG	3 cut(s)	212, 570, 1120
BseSI	GKGCMC	2 cut(s)	568, 1283
BseXI	GCAGC	4 cut(s)	85, 88, 448, 1689
BseYI	CCCAGC	1 cut(s)	68
BsgI	GTGCAG	2 cut(s)	522, 1520
Bsh1236I	CGCG	1 cut(s)	8
Bsh1285I	CGRYCG	1 cut(s)	12
BshFI	GGCC	5 cut(s)	83, 116, 140, 186, 658
BsiEI	CGRYCG	1 cut(s)	12
BsiHKAI	GWGCWC	4 cut(s)	568, 1005, 1283, 1527
BsiHKCI	CYCGRG	2 cut(s)	154, 206
BsiSI	CCGG	6 cut(s)	30, 53, 80, 108, 137, 183
BslFI	GGGAC	6 cut(s)	118, 145, 196, 434, 1364, 1418
BslI	CCNNNNNNNGG	4 cut(s)	257, 617, 1016, 1581
BsmAI	GTCTC	1 cut(s)	517
BsmFI	GGGAC	6 cut(s)	118, 145, 196, 434, 1364, 1418
BsnI	GGCC	5 cut(s)	83, 116, 140, 186, 658
BsoBI	CYCGRG	2 cut(s)	154, 206
Bsp1286I	GDGCHC	4 cut(s)	568, 1005, 1283, 1527
Bsp13I	TCCGGA	1 cut(s)	29
Bsp1720I	GCTNAGC	1 cut(s)	462
BspACI	CCGC	2 cut(s)	12, 1216
BspANI	GGCC	5 cut(s)	83, 116, 140, 186, 658
BspCNI	CTCAG	7 cut(s)	201, 288, 326, 454, 646, 991, 1197
BspEI	TCCGGA	1 cut(s)	29
BspFNI	CGCG	1 cut(s)	8
BspHI	TCATGA	2 cut(s)	796, 1372
BspLI	GGNNCC	5 cut(s)	34, 115, 161, 262, 623
BspPI	GGATC	4 cut(s)	44, 298, 1325, 1657
BsrDI	GCAATG	1 cut(s)	1027
BsrFI	RCCGGY	1 cut(s)	79
BsrI	ACTGG	5 cut(s)	262, 556, 695, 880, 934
BssAI	RCCGGY	1 cut(s)	79
BssECI	CCNNGG	6 cut(s)	135, 345, 451, 570, 617, 1262
BssNI	GRCGYC	2 cut(s)	197, 1377
BssSI	CACGAG	1 cut(s)	1164
BssT1I	CCWWGG	1 cut(s)	1262
Bst2BI	CACGAG	1 cut(s)	1164
Bst2UI	CCWGG	2 cut(s)	453, 618
Bst4CI	ACNGT	4 cut(s)	325, 992, 1053, 1426
Bst6I	CTCTTC	3 cut(s)	97, 520, 1729
BstACI	GRCGYC	2 cut(s)	197, 1377
BstAPI	GCANNNNNTGC	1 cut(s)	442
BstBAI	YACGTR	1 cut(s)	1528
BstC8I	GCNNGC	3 cut(s)	81, 891, 1220
BstDEI	CTNAG	8 cut(s)	188, 296, 313, 462, 633, 846, 999, 1184
BstDSI	CCRYGG	1 cut(s)	570
BstENI	CCTNNNNNAGG	1 cut(s)	1014
BstFNI	CGCG	1 cut(s)	8
BstHHI	GCGC	1 cut(s)	1224
BstMAI	GTCTC	1 cut(s)	517
BstMCI	CGRYCG	1 cut(s)	12
BstMWI	GCNNNNNNNGC	2 cut(s)	442, 787
BstNI	CCWGG	2 cut(s)	453, 618
BstNSI	RCATGY	3 cut(s)	479, 716, 1313
BstSCI	CCNGG	5 cut(s)	52, 135, 181, 451, 616
BstSFI	CTRYAG	2 cut(s)	408, 424
BstSLI	GKGCMC	2 cut(s)	568, 1283
BstUI	CGCG	1 cut(s)	8
BstV1I	GCAGC	4 cut(s)	85, 88, 448, 1689
BstX2I	RGATCY	3 cut(s)	290, 905, 1649
BstYI	RGATCY	3 cut(s)	290, 905, 1649
BsuI	GTATCC	1 cut(s)	1205
BsuRI	GGCC	5 cut(s)	83, 116, 140, 186, 658
BtgI	CCRYGG	1 cut(s)	570
BtgZI	GCGATG	1 cut(s)	1308
BtsIMutI	CAGTG	3 cut(s)	549, 1049, 1192
Cac8I	GCNNGC	3 cut(s)	81, 891, 1220
CciI	TCATGA	2 cut(s)	796, 1372
CfoI	GCGC	1 cut(s)	1224
Cfr10I	RCCGGY	1 cut(s)	79
Cfr13I	GGNCC	8 cut(s)	32, 114, 159, 261, 622, 656, 1415, 1748
CseI	GACGC	1 cut(s)	14
Csp6I	GTAC	7 cut(s)	121, 286, 753, 831, 993, 1711, 1739
CviQI	GTAC	7 cut(s)	121, 286, 753, 831, 993, 1711, 1739
DdeI	CTNAG	8 cut(s)	188, 296, 313, 462, 633, 846, 999, 1184
EaeI	YGGCCR	1 cut(s)	138
Eam1104I	CTCTTC	3 cut(s)	97, 520, 1729
EarI	CTCTTC	3 cut(s)	97, 520, 1729
Ecl136II	GAGCTC	1 cut(s)	1003
Eco130I	CCWWGG	1 cut(s)	1262
Eco24I	GRGCYC	1 cut(s)	1005
Eco47I	GGWCC	6 cut(s)	32, 159, 261, 622, 1415, 1748
Eco53kI	GAGCTC	1 cut(s)	1003
Eco57I	CTGAAG	2 cut(s)	645, 1395
Eco72I	CACGTG	1 cut(s)	1528
Eco88I	CYCGRG	2 cut(s)	154, 206
EcoICRI	GAGCTC	1 cut(s)	1003
EcoNI	CCTNNNNNAGG	1 cut(s)	1014
EcoO109I	RGGNCCY	2 cut(s)	159, 261
EcoRII	CCWGG	2 cut(s)	451, 616
EcoT14I	CCWWGG	1 cut(s)	1262
EcoT22I	ATGCAT	1 cut(s)	481
EcoT38I	GRGCYC	1 cut(s)	1005
ErhI	CCWWGG	1 cut(s)	1262
FaqI	GGGAC	6 cut(s)	118, 145, 196, 434, 1364, 1418
FauNDI	CATATG	1 cut(s)	1354
FbaI	TGATCA	1 cut(s)	838
FblI	GTMKAC	1 cut(s)	862
Fnu4HI	GCNGC	4 cut(s)	99, 102, 437, 1678
FriOI	GRGCYC	1 cut(s)	1005
Fsp4HI	GCNGC	4 cut(s)	99, 102, 437, 1678
FspBI	CTAG	5 cut(s)	390, 561, 972, 1395, 1653
GlaI	GCGC	1 cut(s)	1223
GluI	GCNGC	4 cut(s)	99, 102, 437, 1678
GsaI	CCCAGC	1 cut(s)	72
GsuI	CTGGAG	1 cut(s)	573
HaeIII	GGCC	5 cut(s)	83, 116, 140, 186, 658
HapII	CCGG	6 cut(s)	30, 53, 80, 108, 137, 183
HgaI	GACGC	1 cut(s)	14
HhaI	GCGC	1 cut(s)	1224
Hin1I	GRCGYC	2 cut(s)	197, 1377
Hin6I	GCGC	1 cut(s)	1222
HinP1I	GCGC	1 cut(s)	1222
HincII	GTYRAC	1 cut(s)	863
HindII	GTYRAC	1 cut(s)	863
HindIII	AAGCTT	2 cut(s)	143, 367
HinfI	GANTC	6 cut(s)	209, 581, 1180, 1639, 1670, 1699
HpaII	CCGG	6 cut(s)	30, 53, 80, 108, 137, 183
HphI	GGTGA	5 cut(s)	68, 474, 599, 650, 1255
Hpy166II	GTNNAC	5 cut(s)	547, 566, 833, 863, 1281
Hpy8I	GTNNAC	5 cut(s)	547, 566, 833, 863, 1281
Hpy99I	CGWCG	4 cut(s)	50, 199, 867, 1441
HpyAV	CCTTC	6 cut(s)	36, 229, 255, 669, 1031, 1352
HpyCH4III	ACNGT	4 cut(s)	325, 992, 1053, 1426
HpyCH4IV	ACGT	4 cut(s)	45, 197, 1377, 1527
HpyCH4V	TGCA	8 cut(s)	445, 479, 539, 566, 1281, 1388, 1531, 1537
HpyF10VI	GCNNNNNNNGC	2 cut(s)	442, 787
HpyF3I	CTNAG	8 cut(s)	188, 296, 313, 462, 633, 846, 999, 1184
HpySE526I	ACGT	4 cut(s)	45, 197, 1377, 1527
Hsp92I	GRCGYC	2 cut(s)	197, 1377
HspAI	GCGC	1 cut(s)	1222
Kpn2I	TCCGGA	1 cut(s)	29
KroI	GCCGGC	1 cut(s)	79
KroNI	GCCGGC	1 cut(s)	81
Ksp22I	TGATCA	1 cut(s)	838
LmnI	GCTCC	3 cut(s)	77, 557, 1266
Lsp1109I	GCAGC	4 cut(s)	85, 88, 448, 1689
LweI	GCATC	4 cut(s)	488, 876, 1283, 1331
MaeI	CTAG	5 cut(s)	390, 561, 972, 1395, 1653
MaeII	ACGT	4 cut(s)	45, 197, 1377, 1527
MaeIII	GTNAC	6 cut(s)	403, 587, 974, 1012, 1132, 1460
MboII	GAAGA	7 cut(s)	84, 507, 587, 787, 911, 1466, 1746
MflI	RGATCY	3 cut(s)	290, 905, 1649
MhlI	GDGCHC	4 cut(s)	568, 1005, 1283, 1527
MlyI	GAGTC	2 cut(s)	218, 1174
MmeI	TCCRAC	3 cut(s)	218, 252, 1468
Mph1103I	ATGCAT	1 cut(s)	481
MroI	TCCGGA	1 cut(s)	29
MroNI	GCCGGC	1 cut(s)	79
MseI	TTAA	5 cut(s)	378, 593, 717, 1146, 1626
MslI	CAYNNNNRTG	1 cut(s)	1371
MspA1I	CMGCKG	1 cut(s)	1218
MspI	CCGG	6 cut(s)	30, 53, 80, 108, 137, 183
MspR9I	CCNGG	5 cut(s)	54, 137, 183, 453, 618
MvaI	CCWGG	2 cut(s)	453, 618
MvnI	CGCG	1 cut(s)	8
MwoI	GCNNNNNNNGC	2 cut(s)	442, 787
NaeI	GCCGGC	1 cut(s)	81
NciI	CCSGG	3 cut(s)	54, 137, 183
NdeI	CATATG	1 cut(s)	1354
NgoMIV	GCCGGC	1 cut(s)	79
NlaIV	GGNNCC	5 cut(s)	34, 115, 161, 262, 623
NmuCI	GTSAC	4 cut(s)	587, 1012, 1132, 1460
NsiI	ATGCAT	1 cut(s)	481
NspI	RCATGY	3 cut(s)	479, 716, 1313
PaeR7I	CTCGAG	2 cut(s)	154, 206
PagI	TCATGA	2 cut(s)	796, 1372
PciI	ACATGT	1 cut(s)	1309
PdiI	GCCGGC	1 cut(s)	81
PfeI	GAWTC	4 cut(s)	581, 1639, 1670, 1699
PflMI	CCANNNNNTGG	2 cut(s)	257, 617
PkrI	GCNGC	4 cut(s)	100, 103, 438, 1679
PleI	GAGTC	2 cut(s)	217, 1174
PmaCI	CACGTG	1 cut(s)	1528
PmlI	CACGTG	1 cut(s)	1528
PpsI	GAGTC	2 cut(s)	217, 1174
Ppu21I	YACGTR	1 cut(s)	1528
PpuMI	RGGWCCY	2 cut(s)	159, 261
PscI	ACATGT	1 cut(s)	1309
PsiI	TTATAA	2 cut(s)	417, 1346
Psp124BI	GAGCTC	1 cut(s)	1005
Psp5II	RGGWCCY	2 cut(s)	159, 261
Psp6I	CCWGG	2 cut(s)	451, 616
PspCI	CACGTG	1 cut(s)	1528
PspFI	CCCAGC	1 cut(s)	68
PspGI	CCWGG	2 cut(s)	451, 616
PspN4I	GGNNCC	5 cut(s)	34, 115, 161, 262, 623
PspPI	GGNCC	8 cut(s)	32, 114, 159, 261, 622, 656, 1415, 1748
PspPPI	RGGWCCY	2 cut(s)	159, 261
PsuI	RGATCY	3 cut(s)	290, 905, 1649
RsaI	GTAC	7 cut(s)	122, 287, 754, 832, 994, 1712, 1740
RsaNI	GTAC	7 cut(s)	121, 286, 753, 831, 993, 1711, 1739
RseI	CAYNNNNRTG	1 cut(s)	1371
SacI	GAGCTC	1 cut(s)	1005
SalI	GTCGAC	1 cut(s)	861
SaqAI	TTAA	5 cut(s)	378, 593, 717, 1146, 1626
SatI	GCNGC	4 cut(s)	99, 102, 437, 1678
Sau96I	GGNCC	8 cut(s)	32, 114, 159, 261, 622, 656, 1415, 1748
ScaI	AGTACT	2 cut(s)	994, 1740
SchI	GAGTC	2 cut(s)	218, 1174
ScrFI	CCNGG	5 cut(s)	54, 137, 183, 453, 618
SduI	GDGCHC	4 cut(s)	568, 1005, 1283, 1527
SfaNI	GCATC	4 cut(s)	488, 876, 1283, 1331
SfcI	CTRYAG	2 cut(s)	408, 424
Sfr274I	CTCGAG	2 cut(s)	154, 206
SinI	GGWCC	6 cut(s)	32, 159, 261, 622, 1415, 1748
SlaI	CTCGAG	2 cut(s)	154, 206
SmiMI	CAYNNNNRTG	1 cut(s)	1371
SmlI	CTYRAG	3 cut(s)	154, 206, 894
SmoI	CTYRAG	3 cut(s)	154, 206, 894
SsiI	CCGC	2 cut(s)	12, 1216
SspMI	CTAG	5 cut(s)	390, 561, 972, 1395, 1653
SstI	GAGCTC	1 cut(s)	1005
StyD4I	CCNGG	5 cut(s)	52, 135, 181, 451, 616
StyI	CCWWGG	1 cut(s)	1262
TaaI	ACNGT	4 cut(s)	325, 992, 1053, 1426
TaiI	ACGT	4 cut(s)	48, 200, 1380, 1530
TaqI	TCGA	8 cut(s)	18, 48, 155, 207, 372, 862, 1439, 1607
TatI	WGTACW	3 cut(s)	120, 992, 1738
TfiI	GAWTC	4 cut(s)	581, 1639, 1670, 1699
Tru1I	TTAA	5 cut(s)	378, 593, 717, 1146, 1626
Tru9I	TTAA	5 cut(s)	378, 593, 717, 1146, 1626
TscAI	CASTG	3 cut(s)	556, 1056, 1192
TseFI	GTSAC	4 cut(s)	587, 1012, 1132, 1460
TseI	GCWGC	4 cut(s)	98, 101, 436, 1677
Tsp45I	GTSAC	4 cut(s)	587, 1012, 1132, 1460
TspDTI	ATGAA	6 cut(s)	248, 366, 537, 909, 1371, 1652
TspGWI	ACGGA	2 cut(s)	303, 1740
TspRI	CASTG	3 cut(s)	556, 1056, 1192
Van91I	CCANNNNNTGG	2 cut(s)	257, 617
VneI	GTGCAC	2 cut(s)	564, 1279
VpaK11BI	GGWCC	6 cut(s)	32, 159, 261, 622, 1415, 1748
XagI	CCTNNNNNAGG	1 cut(s)	1014
XapI	RAATTY	3 cut(s)	358, 728, 1686
XceI	RCATGY	3 cut(s)	479, 716, 1313
XhoI	CTCGAG	2 cut(s)	154, 206
XmiI	GTMKAC	1 cut(s)	862
XspI	CTAG	5 cut(s)	390, 561, 972, 1395, 1653
ZraI	GACGTC	2 cut(s)	198, 1378
ZrmI	AGTACT	2 cut(s)	994, 1740
Zsp2I	ATGCAT	1 cut(s)	481

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.