MD03G1199600.v1.1

Senescence regulator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0003172
Physical Location & Seq
Reverse (-)
27302070 .. 27303037
968 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1199600.v1.1.491

Sequence Viewer

Length: 627 bp
ATGGACAATAGGTATGGCCTAATAAGGCAGGGCAGTGGAGTTTGGAGGGCCTTGAGAGATGGAGACTTTGAGGAAGAAGATATTTGGGAGGTTCTAAAAGATAGAAACAATAGTAAAGACATGGCTACTGGATCCAAAAGTTCCAATGTTTCTGTTGCAAGGCACCTCCCAACTGCTGCAAGAATGATTCCAAAACCCAGCAGCCACAATTACGCCAGTGGTAGCAGCTGCAGCAGCAGCAATACTACCAATCATGAAGCCAAAATTGTGCATCAATCAGCACCTGTCAACATTCCTGATTGGTCCAAGTTGTACGGTCAGAAGCCAAACAAGGCCTCCAAGAATGTTTCTTGGCGAAAATATGAGGGCGGTGATGATGATGATGAAGGAAGGGATGATGGCGGCAGAGATAGCAGTGATGATTACGAGGTGGAGGAGGAGGAGGAGGAGGAGGAGGAGTATGATTCAAAGGAACCCCCTCATGAGTTTATTGCTAGGAGGCTTGCAAGGAGTCAGATTTCTTCTTTTTCTGTTTGTGAAGGTGCAGGGAGGACCCTCAAAGGGAGGGATCTCAGCAAAGTGAGGAATGCTGTTTTATCAAAAACTGGATTTCTTGAGTCACTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Senescence_reg

Protein Analysis

209

Amino Acids

23.25

Weight (kDa)

5.17

Isoelectric Point (pI)

54.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Senescence_reg PF04520 22 - 206 1e-35 Senescence regulator
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 162
AciI CCGC 2 cut(s) 369, 402
AclWI GGATC 3 cut(s) 126, 139, 576
AfaI GTAC 1 cut(s) 314
AfiI CCNNNNNNNGG 1 cut(s) 561
AgsI TTSAA 1 cut(s) 468
AluBI AGCT 1 cut(s) 228
AluI AGCT 1 cut(s) 228
Alw26I GTCTC 1 cut(s) 57
AlwI GGATC 3 cut(s) 126, 139, 576
AlwNI CAGNNNCTG 1 cut(s) 284
AoxI GGCC 3 cut(s) 16, 48, 333
ApeKI GCWGC 7 cut(s) 176, 201, 225, 228, 231, 234, 237
AspS9I GGNCC 3 cut(s) 48, 303, 552
AsuHPI GGTGA 1 cut(s) 383
AvaII GGWCC 2 cut(s) 303, 552
BamHI GGATCC 1 cut(s) 131
BanI GGYRCC 1 cut(s) 162
BbvI GCAGC 7 cut(s) 163, 213, 215, 237, 243, 246, 249
BccI CCATC 2 cut(s) 53, 392
BcoDI GTCTC 1 cut(s) 57
BfaI CTAG 1 cut(s) 495
BfmI CTRYAG 1 cut(s) 229
BisI GCNGC 8 cut(s) 177, 202, 226, 229, 232, 235, 238, 403
BlsI GCNGC 8 cut(s) 178, 203, 227, 230, 233, 236, 239, 404
Bme18I GGWCC 2 cut(s) 303, 552
BmgT120I GGNCC 3 cut(s) 48, 303, 552
BmiI GGNNCC 4 cut(s) 133, 164, 474, 554
BmsI GCATC 1 cut(s) 280
BpuEI CTTGAG 1 cut(s) 73
BsaXI ACNNNNNCTCC 4 cut(s) 320, 350, 443, 473
Bsc4I CCNNNNNNNGG 1 cut(s) 561
Bse1I ACTGG 3 cut(s) 133, 216, 610
BseGI GGATG 1 cut(s) 400
BseLI CCNNNNNNNGG 1 cut(s) 561
BseMII CTCAG 1 cut(s) 586
BseNI ACTGG 3 cut(s) 133, 216, 610
BseRI GAGGAG 8 cut(s) 449, 452, 455, 458, 461, 464, 467, 470
BseXI GCAGC 7 cut(s) 163, 213, 215, 237, 243, 246, 249
BseYI CCCAGC 1 cut(s) 197
BsgI GTGCAG 1 cut(s) 564
BshFI GGCC 3 cut(s) 18, 50, 335
BshNI GGYRCC 1 cut(s) 162
BslI CCNNNNNNNGG 1 cut(s) 561
BsmAI GTCTC 1 cut(s) 57
BsmI GAATGC 1 cut(s) 592
BsnI GGCC 3 cut(s) 18, 50, 335
Bsp143I GATC 2 cut(s) 131, 568
BspACI CCGC 2 cut(s) 369, 402
BspANI GGCC 3 cut(s) 18, 50, 335
BspCNI CTCAG 1 cut(s) 585
BspHI TCATGA 2 cut(s) 253, 481
BspLI GGNNCC 4 cut(s) 133, 164, 474, 554
BspMAI CTGCAG 1 cut(s) 233
BspPI GGATC 3 cut(s) 126, 139, 576
BspT107I GGYRCC 1 cut(s) 162
BsrI ACTGG 3 cut(s) 133, 216, 610
BssMI GATC 2 cut(s) 131, 568
Bst4CI ACNGT 1 cut(s) 317
BstC8I GCNNGC 1 cut(s) 504
BstDEI CTNAG 1 cut(s) 572
BstF5I GGATG 1 cut(s) 400
BstKTI GATC 2 cut(s) 134, 571
BstMAI GTCTC 1 cut(s) 57
BstMBI GATC 2 cut(s) 131, 568
BstMWI GCNNNNNNNGC 4 cut(s) 231, 234, 237, 411
BstSFI CTRYAG 1 cut(s) 229
BstV1I GCAGC 7 cut(s) 163, 213, 215, 237, 243, 246, 249
BstX2I RGATCY 2 cut(s) 131, 568
BstYI RGATCY 2 cut(s) 131, 568
BsuRI GGCC 3 cut(s) 18, 50, 335
BtsCI GGATG 1 cut(s) 400
BtsI GCAGTG 2 cut(s) 40, 421
BtsIMutI CAGTG 3 cut(s) 40, 223, 421
Cac8I GCNNGC 1 cut(s) 504
CaiI CAGNNNCTG 1 cut(s) 284
CciI TCATGA 2 cut(s) 253, 481
Cfr13I GGNCC 3 cut(s) 48, 303, 552
Csp6I GTAC 1 cut(s) 313
CviAII CATG 3 cut(s) 121, 254, 482
CviJI RGCY 9 cut(s) 18, 50, 125, 204, 228, 260, 325, 335, 502
CviKI_1 RGCY 9 cut(s) 18, 50, 125, 204, 228, 260, 325, 335, 502
CviQI GTAC 1 cut(s) 313
DdeI CTNAG 1 cut(s) 572
DpnI GATC 2 cut(s) 133, 570
DpnII GATC 2 cut(s) 131, 568
Eco147I AGGCCT 1 cut(s) 335
Eco47I GGWCC 2 cut(s) 303, 552
EcoO109I RGGNCCY 2 cut(s) 48, 552
FaeI CATG 3 cut(s) 124, 257, 485
FaiI YATR 7 cut(s) 15, 122, 255, 363, 462, 483, 625
FatI CATG 3 cut(s) 120, 253, 481
Fnu4HI GCNGC 8 cut(s) 177, 202, 226, 229, 232, 235, 238, 403
FokI GGATG 1 cut(s) 407
Fsp4HI GCNGC 8 cut(s) 177, 202, 226, 229, 232, 235, 238, 403
FspBI CTAG 1 cut(s) 495
GluI GCNGC 8 cut(s) 177, 202, 226, 229, 232, 235, 238, 403
GsaI CCCAGC 1 cut(s) 201
HaeIII GGCC 3 cut(s) 18, 50, 335
Hin1II CATG 3 cut(s) 124, 257, 485
HincII GTYRAC 1 cut(s) 289
HindII GTYRAC 1 cut(s) 289
HinfI GANTC 4 cut(s) 187, 464, 511, 617
HphI GGTGA 1 cut(s) 383
Hpy166II GTNNAC 1 cut(s) 289
Hpy188I TCNGA 2 cut(s) 321, 516
Hpy188III TCNNGA 4 cut(s) 254, 296, 482, 614
Hpy8I GTNNAC 1 cut(s) 289
HpyAV CCTTC 3 cut(s) 380, 384, 533
HpyCH4III ACNGT 1 cut(s) 317
HpyCH4V TGCA 6 cut(s) 158, 179, 231, 271, 506, 545
HpyF10VI GCNNNNNNNGC 4 cut(s) 231, 234, 237, 411
HpyF3I CTNAG 1 cut(s) 572
Hsp92II CATG 3 cut(s) 124, 257, 485
Kzo9I GATC 2 cut(s) 131, 568
LpnPI CCDG 8 cut(s) 14, 114, 211, 229, 297, 309, 531, 591
Lsp1109I GCAGC 7 cut(s) 163, 213, 215, 237, 243, 246, 249
LweI GCATC 1 cut(s) 280
MaeI CTAG 1 cut(s) 495
MaeIII GTNAC 1 cut(s) 618
MalI GATC 2 cut(s) 133, 570
MboI GATC 2 cut(s) 131, 568
MboII GAAGA 3 cut(s) 86, 89, 513
MflI RGATCY 2 cut(s) 131, 568
MluCI AATT 2 cut(s) 208, 264
MlyI GAGTC 2 cut(s) 520, 626
MspA1I CMGCKG 1 cut(s) 228
Mva1269I GAATGC 1 cut(s) 592
MwoI GCNNNNNNNGC 4 cut(s) 231, 234, 237, 411
NdeII GATC 2 cut(s) 131, 568
NlaIII CATG 3 cut(s) 124, 257, 485
NlaIV GGNNCC 4 cut(s) 133, 164, 474, 554
NmuCI GTSAC 1 cut(s) 618
PagI TCATGA 2 cut(s) 253, 481
PceI AGGCCT 1 cut(s) 335
PctI GAATGC 1 cut(s) 592
PfeI GAWTC 2 cut(s) 187, 464
PkrI GCNGC 8 cut(s) 178, 203, 227, 230, 233, 236, 239, 404
PleI GAGTC 2 cut(s) 519, 625
PpsI GAGTC 2 cut(s) 519, 625
PpuMI RGGWCCY 1 cut(s) 552
Psp5II RGGWCCY 1 cut(s) 552
PspFI CCCAGC 1 cut(s) 197
PspN4I GGNNCC 4 cut(s) 133, 164, 474, 554
PspPI GGNCC 3 cut(s) 48, 303, 552
PspPPI RGGWCCY 1 cut(s) 552
PstI CTGCAG 1 cut(s) 233
PstNI CAGNNNCTG 1 cut(s) 284
PsuI RGATCY 2 cut(s) 131, 568
PvuII CAGCTG 1 cut(s) 228
RsaI GTAC 1 cut(s) 314
RsaNI GTAC 1 cut(s) 313
SatI GCNGC 8 cut(s) 177, 202, 226, 229, 232, 235, 238, 403
Sau3AI GATC 2 cut(s) 131, 568
Sau96I GGNCC 3 cut(s) 48, 303, 552
SchI GAGTC 2 cut(s) 520, 626
SetI ASST 7 cut(s) 14, 93, 168, 230, 286, 432, 544
SfaNI GCATC 1 cut(s) 280
SfcI CTRYAG 1 cut(s) 229
SinI GGWCC 2 cut(s) 303, 552
SmlI CTYRAG 2 cut(s) 52, 614
SmoI CTYRAG 2 cut(s) 52, 614
Sse9I AATT 2 cut(s) 208, 264
SseBI AGGCCT 1 cut(s) 335
SsiI CCGC 2 cut(s) 369, 402
SspMI CTAG 1 cut(s) 495
StuI AGGCCT 1 cut(s) 335
TaaI ACNGT 1 cut(s) 317
TasI AATT 2 cut(s) 208, 264
TauI GCSGC 1 cut(s) 405
TfiI GAWTC 2 cut(s) 187, 464
TscAI CASTG 3 cut(s) 40, 223, 421
TseFI GTSAC 1 cut(s) 618
TseI GCWGC 7 cut(s) 176, 201, 225, 228, 231, 234, 237
Tsp45I GTSAC 1 cut(s) 618
TspDTI ATGAA 2 cut(s) 270, 399
TspRI CASTG 3 cut(s) 40, 223, 421
VpaK11BI GGWCC 2 cut(s) 303, 552
XspI CTAG 1 cut(s) 495
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.