Rw5G002210

Senescence regulator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0003172
Physical Location & Seq
Reverse (-)
1866245 .. 1866796
552 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G002210.1

Sequence Viewer

Length: 552 bp
ATGGCAGATTGGAATGGTATGGTGAGCAAAAGCAATGGTTTTAGTGTTGGGGGTTCAAGTGAAGAGTTTGAAGAAGAAGAAGTGTGGGGTGTTGTGAAGGAAAGAAACTACTCCAACTCTAAGCAGGAGTCTTACTCCTCTGGTTCTTCTTCTGCATGGCGCCTCTCTACAGCTCCAAGAACCATCCCAAGGGCTAATAACAACACTCCAAGTACCCATGAAGCCAAAATGGTTCAGAAATCAGCTCCTCTCAACATCCCTGATTGGTCAAAGGTCTATGGGAAAAGTTCTGGTGCTGCCAAGATTAATGGTTCATGGGTCAATAATGAAGATGATCATGATGGACATGTCAATTTCAATGGTGATGATGATGAAAATGATGATGATGATGATGATGATATGGTGCCTCCGCATGAATGGATGGCCAGAAAGCTAGCAAGAAGCCAGATTTCATCTTTCTCAGTTTGTGAAGGGATTGGTAGGACACTCAAAGGAAGAGACCTCAGCAAAGTAAGGAATGCTATTTTGACAAAAACTGGTTTCTTAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Senescence_reg

Protein Analysis

183

Amino Acids

20.22

Weight (kDa)

4.97

Isoelectric Point (pI)

33.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Senescence_reg PF04520 32 - 183 4.1e-38 Senescence regulator
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 159, 403
AciI CCGC 1 cut(s) 410
AcoI YGGCCR 1 cut(s) 423
AcyI GRCGYC 1 cut(s) 160
AfaI GTAC 1 cut(s) 214
AfiI CCNNNNNNNGG 1 cut(s) 189
AflIII ACRYGT 1 cut(s) 346
AgsI TTSAA 3 cut(s) 57, 71, 358
AluBI AGCT 3 cut(s) 173, 245, 433
AluI AGCT 3 cut(s) 173, 245, 433
Alw26I GTCTC 1 cut(s) 492
AoxI GGCC 1 cut(s) 423
ApeKI GCWGC 1 cut(s) 296
AseI ATTAAT 1 cut(s) 306
AspLEI GCGC 1 cut(s) 162
AsuHPI GGTGA 2 cut(s) 34, 374
AsuNHI GCTAGC 1 cut(s) 433
BalI TGGCCA 1 cut(s) 425
BanI GGYRCC 2 cut(s) 159, 403
BbvCI CCTCAGC 1 cut(s) 503
BbvI GCAGC 1 cut(s) 283
BccI CCATC 3 cut(s) 191, 335, 415
BclI TGATCA 1 cut(s) 334
BcoDI GTCTC 1 cut(s) 492
BfaI CTAG 1 cut(s) 434
BfmI CTRYAG 1 cut(s) 168
BfoI RGCGCY 1 cut(s) 163
BisI GCNGC 1 cut(s) 297
BlsI GCNGC 1 cut(s) 298
BmiI GGNNCC 2 cut(s) 161, 405
BmtI GCTAGC 1 cut(s) 437
BplI GAGNNNNNCTC 2 cut(s) 119, 151
Bpu10I CCTNAGC 1 cut(s) 503
BsaHI GRCGYC 1 cut(s) 160
BsaI GGTCTC 1 cut(s) 492
BsaJI CCNNGG 1 cut(s) 188
Bsc4I CCNNNNNNNGG 1 cut(s) 189
Bse1I ACTGG 1 cut(s) 541
Bse3DI GCAATG 1 cut(s) 40
BseDI CCNNGG 1 cut(s) 188
BseGI GGATG 3 cut(s) 183, 255, 426
BseLI CCNNNNNNNGG 1 cut(s) 189
BseMI GCAATG 1 cut(s) 40
BseMII CTCAG 2 cut(s) 474, 517
BseNI ACTGG 1 cut(s) 541
BseRI GAGGAG 2 cut(s) 127, 237
BseXI GCAGC 1 cut(s) 283
BshFI GGCC 1 cut(s) 425
BshNI GGYRCC 2 cut(s) 159, 403
BslI CCNNNNNNNGG 1 cut(s) 189
BsmAI GTCTC 1 cut(s) 492
BsmI GAATGC 1 cut(s) 523
BsnI GGCC 1 cut(s) 425
Bso31I GGTCTC 1 cut(s) 492
Bsp143I GATC 1 cut(s) 334
BspACI CCGC 1 cut(s) 410
BspANI GGCC 1 cut(s) 425
BspCNI CTCAG 2 cut(s) 473, 516
BspHI TCATGA 1 cut(s) 337
BspLI GGNNCC 2 cut(s) 161, 405
BspOI GCTAGC 1 cut(s) 437
BspT107I GGYRCC 2 cut(s) 159, 403
BspTNI GGTCTC 1 cut(s) 492
BsrDI GCAATG 1 cut(s) 40
BsrI ACTGG 1 cut(s) 541
BssECI CCNNGG 1 cut(s) 188
BssMI GATC 1 cut(s) 334
BssNI GRCGYC 1 cut(s) 160
BssT1I CCWWGG 1 cut(s) 188
Bst6I CTCTTC 2 cut(s) 57, 490
BstACI GRCGYC 1 cut(s) 160
BstC8I GCNNGC 1 cut(s) 435
BstDEI CTNAG 4 cut(s) 120, 460, 503, 544
BstF5I GGATG 3 cut(s) 183, 255, 426
BstH2I RGCGCY 1 cut(s) 163
BstHHI GCGC 1 cut(s) 162
BstKTI GATC 1 cut(s) 337
BstMAI GTCTC 1 cut(s) 492
BstMBI GATC 1 cut(s) 334
BstNSI RCATGY 1 cut(s) 350
BstSFI CTRYAG 1 cut(s) 168
BstV1I GCAGC 1 cut(s) 283
BsuRI GGCC 1 cut(s) 425
BtsCI GGATG 3 cut(s) 183, 255, 426
Cac8I GCNNGC 1 cut(s) 435
CciI TCATGA 1 cut(s) 337
CfoI GCGC 1 cut(s) 162
Csp6I GTAC 1 cut(s) 213
CviAII CATG 6 cut(s) 156, 218, 315, 338, 347, 413
CviJI RGCY 7 cut(s) 173, 194, 224, 245, 425, 433, 444
CviKI_1 RGCY 7 cut(s) 173, 194, 224, 245, 425, 433, 444
CviQI GTAC 1 cut(s) 213
DdeI CTNAG 4 cut(s) 120, 460, 503, 544
DinI GGCGCC 1 cut(s) 161
DpnI GATC 1 cut(s) 336
DpnII GATC 1 cut(s) 334
EaeI YGGCCR 1 cut(s) 423
Eam1104I CTCTTC 2 cut(s) 57, 490
EarI CTCTTC 2 cut(s) 57, 490
Eco130I CCWWGG 1 cut(s) 188
Eco31I GGTCTC 1 cut(s) 492
EcoT14I CCWWGG 1 cut(s) 188
EgeI GGCGCC 1 cut(s) 161
EheI GGCGCC 1 cut(s) 161
ErhI CCWWGG 1 cut(s) 188
FaeI CATG 6 cut(s) 159, 221, 318, 341, 350, 416
FaiI YATR 9 cut(s) 20, 157, 219, 279, 316, 339, 348, 401, 414
FatI CATG 6 cut(s) 155, 217, 314, 337, 346, 412
FbaI TGATCA 1 cut(s) 334
Fnu4HI GCNGC 1 cut(s) 297
FokI GGATG 3 cut(s) 170, 242, 433
Fsp4HI GCNGC 1 cut(s) 297
FspBI CTAG 1 cut(s) 434
GlaI GCGC 1 cut(s) 161
GluI GCNGC 1 cut(s) 297
HaeII RGCGCY 1 cut(s) 163
HaeIII GGCC 1 cut(s) 425
HhaI GCGC 1 cut(s) 162
Hin1I GRCGYC 1 cut(s) 160
Hin1II CATG 6 cut(s) 159, 221, 318, 341, 350, 416
Hin6I GCGC 1 cut(s) 160
HinP1I GCGC 1 cut(s) 160
HinfI GANTC 1 cut(s) 128
HphI GGTGA 2 cut(s) 34, 374
Hpy188I TCNGA 1 cut(s) 237
Hpy188III TCNNGA 1 cut(s) 338
HpyAV CCTTC 2 cut(s) 91, 464
HpyCH4V TGCA 1 cut(s) 155
HpyF3I CTNAG 4 cut(s) 120, 460, 503, 544
Hsp92I GRCGYC 1 cut(s) 160
Hsp92II CATG 6 cut(s) 159, 221, 318, 341, 350, 416
HspAI GCGC 1 cut(s) 160
KasI GGCGCC 1 cut(s) 159
Ksp22I TGATCA 1 cut(s) 334
Kzo9I GATC 1 cut(s) 334
LmnI GCTCC 2 cut(s) 178, 250
LpnPI CCDG 7 cut(s) 110, 126, 273, 276, 439, 458, 522
Lsp1109I GCAGC 1 cut(s) 283
MaeI CTAG 1 cut(s) 434
MalI GATC 1 cut(s) 336
MboI GATC 1 cut(s) 334
MboII GAAGA 8 cut(s) 74, 83, 86, 89, 138, 141, 341, 507
MlsI TGGCCA 1 cut(s) 425
MluCI AATT 1 cut(s) 352
MluNI TGGCCA 1 cut(s) 425
Mly113I GGCGCC 1 cut(s) 160
MlyI GAGTC 1 cut(s) 137
MmeI TCCRAC 1 cut(s) 138
MnlI CCTC 5 cut(s) 148, 173, 258, 417, 512
Mox20I TGGCCA 1 cut(s) 425
MscI TGGCCA 1 cut(s) 425
MseI TTAA 1 cut(s) 306
Msp20I TGGCCA 1 cut(s) 425
Mva1269I GAATGC 1 cut(s) 523
NarI GGCGCC 1 cut(s) 160
NdeII GATC 1 cut(s) 334
NheI GCTAGC 1 cut(s) 433
NlaIII CATG 6 cut(s) 159, 221, 318, 341, 350, 416
NlaIV GGNNCC 2 cut(s) 161, 405
NspI RCATGY 1 cut(s) 350
PagI TCATGA 1 cut(s) 337
PciI ACATGT 1 cut(s) 346
PctI GAATGC 1 cut(s) 523
PkrI GCNGC 1 cut(s) 298
PleI GAGTC 1 cut(s) 136
PluTI GGCGCC 1 cut(s) 163
PpsI GAGTC 1 cut(s) 136
PscI ACATGT 1 cut(s) 346
PshBI ATTAAT 1 cut(s) 306
PspN4I GGNNCC 2 cut(s) 161, 405
RsaI GTAC 1 cut(s) 214
RsaNI GTAC 1 cut(s) 213
SaqAI TTAA 1 cut(s) 306
SatI GCNGC 1 cut(s) 297
Sau3AI GATC 1 cut(s) 334
SchI GAGTC 1 cut(s) 137
SetI ASST 5 cut(s) 175, 247, 276, 435, 504
SfcI CTRYAG 1 cut(s) 168
SfoI GGCGCC 1 cut(s) 161
Sse9I AATT 1 cut(s) 352
SsiI CCGC 1 cut(s) 410
SspDI GGCGCC 1 cut(s) 159
SspMI CTAG 1 cut(s) 434
StyI CCWWGG 1 cut(s) 188
TasI AATT 1 cut(s) 352
Tru1I TTAA 1 cut(s) 306
Tru9I TTAA 1 cut(s) 306
TseI GCWGC 1 cut(s) 296
TspDTI ATGAA 6 cut(s) 234, 303, 342, 387, 429, 441
VspI ATTAAT 1 cut(s) 306
XceI RCATGY 1 cut(s) 350
XspI CTAG 1 cut(s) 434
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.