MD03G1243800.v1.1

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0004319
Physical Location & Seq
Reverse (-)
32805768 .. 32808208
2441 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1243800.v1.1.491

Sequence Viewer

Length: 549 bp
ATGGCCCACTACGCCGAGTACTACCGTGTCAAGTCTCTTGCCGCGGAGCGCGACACCCTCTCCGTCTTTGTTGCTCCCTGGGCCACCACCCTCGAGCGCTCCCTCTACTGGATTGGCTGCTGGCGCCCCACCACTGCCTTCCACCTTATCTACTCCGAGTCCTCCATCCACTTTGAGGCTCACATCTTCGACATACTCCAGGGCAACGGCACCGGTGACCTCGGGGACCTCACCCCCTACCAGTTCGGCCGCGTCAGCGAACTCCAATGCGAAACTGTGAAAGAGGAGAATGCGATATCGGAGCAGTTATCAGAGTGGCAAGCCGAGGCGTTTGAGATGATAGGCGCATGCACGAATCTGGATATGAACATGGGATCGTTGGTGAGTGTGTTGAAGACGGCGGACGATCTGCGGCTGAGGACGGTGAGAAAAATTGTGGAACTGCTGACTCCACAGCAGGCTGTGGAGTTCTTGATTGCAGCTGCCGAGTTGCAGTTTGGGGTCCGTGGTTGGGGGATGAATCAGGACCGTCGGCGTGGGAATGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DOG1

Protein Analysis

183

Amino Acids

20.64

Weight (kDa)

5.0

Isoelectric Point (pI)

41.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DOG1 PF14144 1 - 52 1.3e-19 Seed dormancy control
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 123, 209
AccII CGCG 3 cut(s) 44, 51, 252
AciI CCGC 5 cut(s) 42, 44, 250, 401, 412
AclWI GGATC 1 cut(s) 382
AcoI YGGCCR 1 cut(s) 247
AcyI GRCGYC 1 cut(s) 124
AfaI GTAC 1 cut(s) 20
AfeI AGCGCT 1 cut(s) 98
AfiI CCNNNNNNNGG 3 cut(s) 108, 175, 511
AgeI ACCGGT 1 cut(s) 212
AgsI TTSAA 1 cut(s) 394
AjnI CCWGG 2 cut(s) 77, 198
AluBI AGCT 1 cut(s) 482
AluI AGCT 1 cut(s) 482
Alw26I GTCTC 1 cut(s) 39
AlwI GGATC 1 cut(s) 382
Ama87I CYCGRG 2 cut(s) 92, 221
Aor51HI AGCGCT 1 cut(s) 98
AoxI GGCC 3 cut(s) 3, 81, 247
ApeKI GCWGC 3 cut(s) 117, 479, 482
AsiGI ACCGGT 1 cut(s) 212
AspLEI GCGC 4 cut(s) 51, 99, 126, 347
AspS9I GGNCC 5 cut(s) 4, 81, 226, 502, 526
AsuHPI GGTGA 4 cut(s) 223, 227, 394, 436
AvaI CYCGRG 2 cut(s) 92, 221
AvaII GGWCC 3 cut(s) 226, 502, 526
BanI GGYRCC 2 cut(s) 123, 209
BbsI GAAGAC 1 cut(s) 401
BbvCI CCTCAGC 1 cut(s) 416
BbvI GCAGC 3 cut(s) 104, 469, 491
BccI CCATC 1 cut(s) 173
BceAI ACGGC 2 cut(s) 223, 414
BciT130I CCWGG 2 cut(s) 79, 200
BcoDI GTCTC 1 cut(s) 39
BfoI RGCGCY 2 cut(s) 100, 127
BisI GCNGC 6 cut(s) 42, 118, 250, 413, 480, 483
BlsI GCNGC 6 cut(s) 43, 119, 251, 414, 481, 484
BmcAI AGTACT 1 cut(s) 20
Bme1390I CCNGG 2 cut(s) 79, 200
Bme18I GGWCC 3 cut(s) 226, 502, 526
BmeT110I CYCGRG 2 cut(s) 92, 221
BmgT120I GGNCC 5 cut(s) 4, 81, 226, 502, 526
BmiI GGNNCC 4 cut(s) 125, 211, 227, 503
BmrFI CCNGG 2 cut(s) 79, 200
BpiI GAAGAC 1 cut(s) 401
BpmI CTGGAG 1 cut(s) 182
Bpu10I CCTNAGC 1 cut(s) 416
BsaHI GRCGYC 1 cut(s) 124
BsaJI CCNNGG 7 cut(s) 42, 77, 78, 199, 220, 324, 505
BsaWI WCCGGW 1 cut(s) 212
BsaXI ACNNNNNCTCC 2 cut(s) 44, 74
Bsc4I CCNNNNNNNGG 3 cut(s) 108, 175, 511
Bse118I RCCGGY 1 cut(s) 212
Bse1I ACTGG 2 cut(s) 113, 241
BseBI CCWGG 2 cut(s) 79, 200
BseDI CCNNGG 7 cut(s) 42, 77, 78, 199, 220, 324, 505
BseGI GGATG 2 cut(s) 165, 522
BseLI CCNNNNNNNGG 3 cut(s) 108, 175, 511
BseMII CTCAG 1 cut(s) 407
BseNI ACTGG 2 cut(s) 113, 241
BseRI GAGGAG 1 cut(s) 299
BseX3I CGGCCG 1 cut(s) 247
BseXI GCAGC 3 cut(s) 104, 469, 491
Bsh1236I CGCG 3 cut(s) 44, 51, 252
Bsh1285I CGRYCG 1 cut(s) 250
BshFI GGCC 3 cut(s) 5, 83, 249
BshNI GGYRCC 2 cut(s) 123, 209
BshTI ACCGGT 1 cut(s) 212
BsiEI CGRYCG 1 cut(s) 250
BsiHKCI CYCGRG 2 cut(s) 92, 221
BsiSI CCGG 1 cut(s) 213
BslFI GGGAC 1 cut(s) 239
BslI CCNNNNNNNGG 3 cut(s) 108, 175, 511
BsmAI GTCTC 1 cut(s) 39
BsmFI GGGAC 1 cut(s) 239
BsmI GAATGC 1 cut(s) 295
BsnI GGCC 3 cut(s) 5, 83, 249
BsoBI CYCGRG 2 cut(s) 92, 221
Bsp143I GATC 2 cut(s) 374, 406
BspACI CCGC 5 cut(s) 42, 44, 250, 401, 412
BspANI GGCC 3 cut(s) 5, 83, 249
BspCNI CTCAG 1 cut(s) 408
BspFNI CGCG 3 cut(s) 44, 51, 252
BspLI GGNNCC 4 cut(s) 125, 211, 227, 503
BspPI GGATC 1 cut(s) 382
BspT107I GGYRCC 2 cut(s) 123, 209
BsrFI RCCGGY 1 cut(s) 212
BsrI ACTGG 2 cut(s) 113, 241
BssAI RCCGGY 1 cut(s) 212
BssECI CCNNGG 7 cut(s) 42, 77, 78, 199, 220, 324, 505
BssMI GATC 2 cut(s) 374, 406
BssNI GRCGYC 1 cut(s) 124
Bst2UI CCWGG 2 cut(s) 79, 200
Bst4CI ACNGT 4 cut(s) 26, 277, 424, 530
BstACI GRCGYC 1 cut(s) 124
BstC8I GCNNGC 4 cut(s) 122, 321, 349, 459
BstDEI CTNAG 1 cut(s) 416
BstDSI CCRYGG 2 cut(s) 42, 505
BstEII GGTNACC 1 cut(s) 215
BstF5I GGATG 2 cut(s) 165, 522
BstFNI CGCG 3 cut(s) 44, 51, 252
BstH2I RGCGCY 2 cut(s) 100, 127
BstHHI GCGC 4 cut(s) 51, 99, 126, 347
BstKTI GATC 2 cut(s) 377, 409
BstMAI GTCTC 1 cut(s) 39
BstMBI GATC 2 cut(s) 374, 406
BstMCI CGRYCG 1 cut(s) 250
BstMWI GCNNNNNNNGC 4 cut(s) 11, 80, 123, 255
BstNI CCWGG 2 cut(s) 79, 200
BstNSI RCATGY 1 cut(s) 351
BstPI GGTNACC 1 cut(s) 215
BstSCI CCNGG 2 cut(s) 77, 198
BstUI CGCG 3 cut(s) 44, 51, 252
BstV1I GCAGC 3 cut(s) 104, 469, 491
BstV2I GAAGAC 1 cut(s) 401
BstZI CGGCCG 1 cut(s) 247
BsuRI GGCC 3 cut(s) 5, 83, 249
BtgI CCRYGG 2 cut(s) 42, 505
BtsCI GGATG 2 cut(s) 165, 522
BtsI GCAGTG 1 cut(s) 132
BtsIMutI CAGTG 1 cut(s) 132
Cac8I GCNNGC 4 cut(s) 122, 321, 349, 459
CfoI GCGC 4 cut(s) 51, 99, 126, 347
Cfr10I RCCGGY 1 cut(s) 212
Cfr13I GGNCC 5 cut(s) 4, 81, 226, 502, 526
Cfr42I CCGCGG 1 cut(s) 45
CseI GACGC 1 cut(s) 241
Csp6I GTAC 1 cut(s) 19
CspAI ACCGGT 1 cut(s) 212
CviAII CATG 2 cut(s) 348, 370
CviJI RGCY 9 cut(s) 5, 83, 117, 179, 249, 323, 415, 461, 482
CviKI_1 RGCY 9 cut(s) 5, 83, 117, 179, 249, 323, 415, 461, 482
CviQI GTAC 1 cut(s) 19
DdeI CTNAG 1 cut(s) 416
DinI GGCGCC 1 cut(s) 125
DpnI GATC 2 cut(s) 376, 408
DpnII GATC 2 cut(s) 374, 406
EaeI YGGCCR 1 cut(s) 247
EagI CGGCCG 1 cut(s) 247
EciI GGCGGA 1 cut(s) 416
EclXI CGGCCG 1 cut(s) 247
Eco32I GATATC 1 cut(s) 297
Eco47I GGWCC 3 cut(s) 226, 502, 526
Eco47III AGCGCT 1 cut(s) 98
Eco52I CGGCCG 1 cut(s) 247
Eco88I CYCGRG 2 cut(s) 92, 221
Eco91I GGTNACC 1 cut(s) 215
EcoO109I RGGNCCY 1 cut(s) 226
EcoO65I GGTNACC 1 cut(s) 215
EcoRII CCWGG 2 cut(s) 77, 198
EcoRV GATATC 1 cut(s) 297
EgeI GGCGCC 1 cut(s) 125
EheI GGCGCC 1 cut(s) 125
FaeI CATG 2 cut(s) 351, 373
FaiI YATR 4 cut(s) 194, 349, 365, 371
FaqI GGGAC 1 cut(s) 239
FatI CATG 2 cut(s) 347, 369
Fnu4HI GCNGC 6 cut(s) 42, 118, 250, 413, 480, 483
FokI GGATG 2 cut(s) 152, 529
Fsp4HI GCNGC 6 cut(s) 42, 118, 250, 413, 480, 483
GlaI GCGC 4 cut(s) 50, 98, 125, 346
GluI GCNGC 6 cut(s) 42, 118, 250, 413, 480, 483
GsuI CTGGAG 1 cut(s) 182
HaeII RGCGCY 2 cut(s) 100, 127
HaeIII GGCC 3 cut(s) 5, 83, 249
HapII CCGG 1 cut(s) 213
HgaI GACGC 1 cut(s) 241
HhaI GCGC 4 cut(s) 51, 99, 126, 347
Hin1I GRCGYC 1 cut(s) 124
Hin1II CATG 2 cut(s) 351, 373
Hin6I GCGC 4 cut(s) 49, 97, 124, 345
HinP1I GCGC 4 cut(s) 49, 97, 124, 345
HinfI GANTC 4 cut(s) 158, 355, 448, 520
HpaII CCGG 1 cut(s) 213
HphI GGTGA 4 cut(s) 223, 227, 394, 436
Hpy188I TCNGA 4 cut(s) 157, 301, 313, 548
Hpy188III TCNNGA 3 cut(s) 359, 472, 524
Hpy99I CGWCG 1 cut(s) 534
HpyAV CCTTC 1 cut(s) 148
HpyCH4III ACNGT 4 cut(s) 26, 277, 424, 530
HpyCH4V TGCA 3 cut(s) 351, 479, 493
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 80, 123, 255
HpyF3I CTNAG 1 cut(s) 416
Hsp92I GRCGYC 1 cut(s) 124
Hsp92II CATG 2 cut(s) 351, 373
HspAI GCGC 4 cut(s) 49, 97, 124, 345
KasI GGCGCC 1 cut(s) 123
KspI CCGCGG 1 cut(s) 45
Kzo9I GATC 2 cut(s) 374, 406
LmnI GCTCC 4 cut(s) 46, 79, 104, 301
Lsp1109I GCAGC 3 cut(s) 104, 469, 491
MaeIII GTNAC 1 cut(s) 215
MalI GATC 2 cut(s) 376, 408
MboI GATC 2 cut(s) 374, 406
MboII GAAGA 2 cut(s) 178, 406
MluCI AATT 1 cut(s) 432
Mly113I GGCGCC 1 cut(s) 124
MlyI GAGTC 2 cut(s) 167, 442
MspA1I CMGCKG 2 cut(s) 44, 482
MspI CCGG 1 cut(s) 213
MspR9I CCNGG 2 cut(s) 79, 200
Mva1269I GAATGC 1 cut(s) 295
MvaI CCWGG 2 cut(s) 79, 200
MvnI CGCG 3 cut(s) 44, 51, 252
MwoI GCNNNNNNNGC 4 cut(s) 11, 80, 123, 255
NarI GGCGCC 1 cut(s) 124
NdeII GATC 2 cut(s) 374, 406
NlaIII CATG 2 cut(s) 351, 373
NlaIV GGNNCC 4 cut(s) 125, 211, 227, 503
NmeAIII GCCGAG 3 cut(s) 40, 349, 511
NmuCI GTSAC 1 cut(s) 215
NspI RCATGY 1 cut(s) 351
PaeI GCATGC 1 cut(s) 351
PaeR7I CTCGAG 1 cut(s) 92
PasI CCCWGGG 1 cut(s) 78
PctI GAATGC 1 cut(s) 295
PfeI GAWTC 2 cut(s) 355, 520
PinAI ACCGGT 1 cut(s) 212
PkrI GCNGC 6 cut(s) 43, 119, 251, 414, 481, 484
PleI GAGTC 2 cut(s) 166, 442
PluTI GGCGCC 1 cut(s) 127
PpsI GAGTC 2 cut(s) 166, 442
PpuMI RGGWCCY 1 cut(s) 226
Psp5II RGGWCCY 1 cut(s) 226
Psp6I CCWGG 2 cut(s) 77, 198
PspEI GGTNACC 1 cut(s) 215
PspGI CCWGG 2 cut(s) 77, 198
PspN4I GGNNCC 4 cut(s) 125, 211, 227, 503
PspPI GGNCC 5 cut(s) 4, 81, 226, 502, 526
PspPPI RGGWCCY 1 cut(s) 226
PspXI VCTCGAGB 1 cut(s) 92
PvuII CAGCTG 1 cut(s) 482
RsaI GTAC 1 cut(s) 20
RsaNI GTAC 1 cut(s) 19
SacII CCGCGG 1 cut(s) 45
SatI GCNGC 6 cut(s) 42, 118, 250, 413, 480, 483
Sau3AI GATC 2 cut(s) 374, 406
Sau96I GGNCC 5 cut(s) 4, 81, 226, 502, 526
ScaI AGTACT 1 cut(s) 20
SchI GAGTC 2 cut(s) 167, 442
ScrFI CCNGG 2 cut(s) 79, 200
SetI ASST 4 cut(s) 147, 222, 231, 484
SfoI GGCGCC 1 cut(s) 125
Sfr274I CTCGAG 1 cut(s) 92
Sfr303I CCGCGG 1 cut(s) 45
SgrAI CRCCGGYG 1 cut(s) 212
SgrBI CCGCGG 1 cut(s) 45
SinI GGWCC 3 cut(s) 226, 502, 526
SlaI CTCGAG 1 cut(s) 92
SmlI CTYRAG 1 cut(s) 92
SmoI CTYRAG 1 cut(s) 92
SphI GCATGC 1 cut(s) 351
Sse9I AATT 1 cut(s) 432
SsiI CCGC 5 cut(s) 42, 44, 250, 401, 412
SspDI GGCGCC 1 cut(s) 123
StyD4I CCNGG 2 cut(s) 77, 198
TaaI ACNGT 4 cut(s) 26, 277, 424, 530
TaqI TCGA 2 cut(s) 93, 189
TasI AATT 1 cut(s) 432
TatI WGTACW 1 cut(s) 18
TauI GCSGC 3 cut(s) 44, 252, 415
TfiI GAWTC 2 cut(s) 355, 520
TscAI CASTG 1 cut(s) 139
TseFI GTSAC 1 cut(s) 215
TseI GCWGC 3 cut(s) 117, 479, 482
Tsp45I GTSAC 1 cut(s) 215
TspDTI ATGAA 2 cut(s) 380, 533
TspGWI ACGGA 2 cut(s) 52, 494
TspRI CASTG 1 cut(s) 139
VpaK11BI GGWCC 3 cut(s) 226, 502, 526
XceI RCATGY 1 cut(s) 351
XhoI CTCGAG 1 cut(s) 92
ZrmI AGTACT 1 cut(s) 20
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.