MD15G1138000.v1.1

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0004319
Physical Location & Seq
Forward (+)
10127426 .. 10128280
855 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1138000.v1.1.491

Sequence Viewer

Length: 855 bp
ATGGCAAACACCAACATTTTAGAAGTTGAGCGAGAGAGCTTCCACAGGTTCTTTGAGTGCTGGCTTGCTGAACAAAACCAGCACCTCCACGACCTCATTACTGCCGCCAAAAATAACACCACCTCCGCCGCTACTAATCCTACAACCACTGCTGTTATTAATACTAGCAGTAGAAGTTTAGGAACACTGGTGGAGCGCGTGGTGAAGCACTACGAGCATTACTACGAGGTAAAGTCGAGATGGGTTAAGCAAGACGTCCTTGGGATGCTGTCGCCTTCTTGGCTGAGCTCCCTCGAGGACGCCTTCCTTTGGATTGGCGGCTGGAGACCAAGCATGGCTTTTCATCTCTTCCACTCAAAGTCGGGATTGCAACTCGAGGTTCGAGTCGCGGAGTTTATCCGAGGCTTTTGTAGTAGTGGTAGTAAGGGTGACTTGGCGGATTTGTCGTCACATCAGCTCACGCAAGTGGACCAGATGCAGCAGAGGACTGTTAAGGAAGAGAGGGACATCAGCGAGAAGATGGCCAAACGGCAGGAGGCCGTCGCTGACTCCTCCTTGGTCGGCTTGTCCCACGTCATTACGGAGTTGATGGATGCCGGTAACGGTGTTGATGATCCGGTTGCGGAGGAGGACCCCCGAGTGGAATACGCTCTGGCATCCAAGGAGGAAGGTTTGGAGGAGGTTCTGCATAGGGCTGATAATCTACGGCTGATGACGCTGAAAGCTATCACTCACATTCTGACTCCAATCCAGGCAGTCCATTTCTTGATTGCTGCTGCTGAGTTGCACTTGAGGCTTCATGACTGGGGAAAAAAGAAAGACGCCACCACCCGTGCCCAATCTTCTCAACCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DOG1

Protein Analysis

285

Amino Acids

31.97

Weight (kDa)

5.86

Isoelectric Point (pI)

48.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DOG1 PF14144 55 - 117 4.3e-25 Seed dormancy control
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 258
AbsI CCTCGAGG 1 cut(s) 293
AccII CGCG 2 cut(s) 198, 389
AciI CCGC 7 cut(s) 105, 126, 129, 318, 389, 437, 623
AclWI GGATC 1 cut(s) 608
AcoI YGGCCR 1 cut(s) 522
AcyI GRCGYC 3 cut(s) 255, 300, 822
AfiI CCNNNNNNNGG 2 cut(s) 309, 640
AjiI CACGTC 1 cut(s) 574
AjnI CCWGG 1 cut(s) 750
AleI CACNNNNGTG 1 cut(s) 464
AluBI AGCT 4 cut(s) 39, 288, 457, 725
AluI AGCT 4 cut(s) 39, 288, 457, 725
Alw21I GWGCWC 1 cut(s) 290
Alw26I GTCTC 1 cut(s) 319
AlwI GGATC 1 cut(s) 608
Ama87I CYCGRG 3 cut(s) 293, 374, 636
AoxI GGCC 2 cut(s) 522, 537
ApeKI GCWGC 3 cut(s) 478, 773, 776
AseI ATTAAT 1 cut(s) 159
AspLEI GCGC 1 cut(s) 198
AspS9I GGNCC 2 cut(s) 469, 631
AsuHPI GGTGA 2 cut(s) 214, 440
AvaI CYCGRG 3 cut(s) 293, 374, 636
AvaII GGWCC 2 cut(s) 469, 631
BaeGI GKGCMC 1 cut(s) 838
BalI TGGCCA 1 cut(s) 524
BanII GRGCYC 1 cut(s) 290
Bbv12I GWGCWC 1 cut(s) 290
BbvI GCAGC 3 cut(s) 490, 760, 763
BccI CCATC 3 cut(s) 234, 514, 583
BceAI ACGGC 3 cut(s) 524, 545, 722
BciT130I CCWGG 1 cut(s) 752
BcoDI GTCTC 1 cut(s) 319
BfaI CTAG 1 cut(s) 165
BglI GCCNNNNNGGC 1 cut(s) 280
BisI GCNGC 6 cut(s) 105, 129, 319, 479, 774, 777
BlpI GCTNAGC 1 cut(s) 284
BlsI GCNGC 6 cut(s) 106, 130, 320, 480, 775, 778
Bme1390I CCNGG 1 cut(s) 752
Bme18I GGWCC 2 cut(s) 469, 631
BmeT110I CYCGRG 3 cut(s) 293, 374, 636
BmgBI CACGTC 1 cut(s) 574
BmgT120I GGNCC 2 cut(s) 469, 631
BmiI GGNNCC 1 cut(s) 633
BmrFI CCNGG 1 cut(s) 752
BmrI ACTGGG 1 cut(s) 814
BmsI GCATC 4 cut(s) 255, 465, 583, 665
BmuI ACTGGG 1 cut(s) 814
BpmI CTGGAG 1 cut(s) 343
Bpu1102I GCTNAGC 1 cut(s) 284
BpuEI CTTGAG 1 cut(s) 811
BsaHI GRCGYC 3 cut(s) 255, 300, 822
BsaI GGTCTC 1 cut(s) 319
BsaJI CCNNGG 4 cut(s) 259, 400, 555, 660
BsaWI WCCGGW 1 cut(s) 616
BsaXI ACNNNNNCTCC 2 cut(s) 107, 137
Bsc4I CCNNNNNNNGG 2 cut(s) 309, 640
Bse118I RCCGGY 1 cut(s) 596
Bse1I ACTGG 2 cut(s) 192, 809
BseBI CCWGG 1 cut(s) 752
BseDI CCNNGG 4 cut(s) 259, 400, 555, 660
BseGI GGATG 3 cut(s) 270, 598, 656
BseLI CCNNNNNNNGG 2 cut(s) 309, 640
BseMII CTCAG 2 cut(s) 275, 771
BseNI ACTGG 2 cut(s) 192, 809
BseRI GAGGAG 3 cut(s) 541, 641, 692
BseSI GKGCMC 1 cut(s) 838
BseXI GCAGC 3 cut(s) 490, 760, 763
Bsh1236I CGCG 2 cut(s) 198, 389
BshFI GGCC 2 cut(s) 524, 539
BsiHKAI GWGCWC 1 cut(s) 290
BsiHKCI CYCGRG 3 cut(s) 293, 374, 636
BsiSI CCGG 2 cut(s) 597, 617
BslFI GGGAC 2 cut(s) 518, 553
BslI CCNNNNNNNGG 2 cut(s) 309, 640
BsmAI GTCTC 1 cut(s) 319
BsmFI GGGAC 2 cut(s) 518, 553
BsnI GGCC 2 cut(s) 524, 539
Bso31I GGTCTC 1 cut(s) 319
BsoBI CYCGRG 3 cut(s) 293, 374, 636
Bsp1286I GDGCHC 2 cut(s) 290, 838
Bsp143I GATC 1 cut(s) 613
Bsp1720I GCTNAGC 1 cut(s) 284
BspACI CCGC 7 cut(s) 105, 126, 129, 318, 389, 437, 623
BspANI GGCC 2 cut(s) 524, 539
BspCNI CTCAG 2 cut(s) 276, 772
BspFNI CGCG 2 cut(s) 198, 389
BspHI TCATGA 1 cut(s) 799
BspLI GGNNCC 1 cut(s) 633
BspPI GGATC 1 cut(s) 608
BspTNI GGTCTC 1 cut(s) 319
BsrFI RCCGGY 1 cut(s) 596
BsrI ACTGG 2 cut(s) 192, 809
BssAI RCCGGY 1 cut(s) 596
BssECI CCNNGG 4 cut(s) 259, 400, 555, 660
BssMI GATC 1 cut(s) 613
BssNI GRCGYC 3 cut(s) 255, 300, 822
BssT1I CCWWGG 3 cut(s) 259, 555, 660
Bst2UI CCWGG 1 cut(s) 752
Bst4CI ACNGT 2 cut(s) 490, 605
Bst6I CTCTTC 2 cut(s) 353, 492
BstACI GRCGYC 3 cut(s) 255, 300, 822
BstC8I GCNNGC 2 cut(s) 62, 66
BstDEI CTNAG 2 cut(s) 284, 780
BstF5I GGATG 3 cut(s) 270, 598, 656
BstFNI CGCG 2 cut(s) 198, 389
BstHHI GCGC 1 cut(s) 198
BstKTI GATC 1 cut(s) 616
BstMAI GTCTC 1 cut(s) 319
BstMBI GATC 1 cut(s) 613
BstMWI GCNNNNNNNGC 4 cut(s) 214, 280, 715, 793
BstNI CCWGG 1 cut(s) 752
BstSCI CCNGG 1 cut(s) 750
BstSLI GKGCMC 1 cut(s) 838
BstUI CGCG 2 cut(s) 198, 389
BstV1I GCAGC 3 cut(s) 490, 760, 763
BsuRI GGCC 2 cut(s) 524, 539
BtrI CACGTC 1 cut(s) 574
BtsCI GGATG 3 cut(s) 270, 598, 656
BtsI GCAGTG 1 cut(s) 147
BtsIMutI CAGTG 2 cut(s) 147, 185
Cac8I GCNNGC 2 cut(s) 62, 66
CciI TCATGA 1 cut(s) 799
CfoI GCGC 1 cut(s) 198
Cfr10I RCCGGY 1 cut(s) 596
Cfr13I GGNCC 2 cut(s) 469, 631
CseI GACGC 3 cut(s) 308, 724, 830
CviAII CATG 3 cut(s) 334, 800, 852
DdeI CTNAG 2 cut(s) 284, 780
DpnI GATC 1 cut(s) 615
DpnII GATC 1 cut(s) 613
EaeI YGGCCR 1 cut(s) 522
Eam1104I CTCTTC 2 cut(s) 353, 492
EarI CTCTTC 2 cut(s) 353, 492
EciI GGCGGA 2 cut(s) 115, 452
Ecl136II GAGCTC 1 cut(s) 288
Eco130I CCWWGG 3 cut(s) 259, 555, 660
Eco24I GRGCYC 1 cut(s) 290
Eco31I GGTCTC 1 cut(s) 319
Eco47I GGWCC 2 cut(s) 469, 631
Eco53kI GAGCTC 1 cut(s) 288
Eco88I CYCGRG 3 cut(s) 293, 374, 636
EcoICRI GAGCTC 1 cut(s) 288
EcoO109I RGGNCCY 1 cut(s) 631
EcoRII CCWGG 1 cut(s) 750
EcoT14I CCWWGG 3 cut(s) 259, 555, 660
EcoT38I GRGCYC 1 cut(s) 290
ErhI CCWWGG 3 cut(s) 259, 555, 660
FaeI CATG 3 cut(s) 337, 803, 855
FaiI YATR 4 cut(s) 335, 690, 801, 853
FalI AAGNNNNNCTT 6 cut(s) 243, 275, 322, 354, 416, 448
FaqI GGGAC 2 cut(s) 518, 553
FatI CATG 3 cut(s) 333, 799, 851
Fnu4HI GCNGC 6 cut(s) 105, 129, 319, 479, 774, 777
FokI GGATG 3 cut(s) 277, 605, 643
FriOI GRGCYC 1 cut(s) 290
Fsp4HI GCNGC 6 cut(s) 105, 129, 319, 479, 774, 777
FspBI CTAG 1 cut(s) 165
GlaI GCGC 1 cut(s) 197
GluI GCNGC 6 cut(s) 105, 129, 319, 479, 774, 777
GsuI CTGGAG 1 cut(s) 343
HaeIII GGCC 2 cut(s) 524, 539
HapII CCGG 2 cut(s) 597, 617
HgaI GACGC 3 cut(s) 308, 724, 830
HhaI GCGC 1 cut(s) 198
Hin1I GRCGYC 3 cut(s) 255, 300, 822
Hin1II CATG 3 cut(s) 337, 803, 855
Hin6I GCGC 1 cut(s) 196
HinP1I GCGC 1 cut(s) 196
HinfI GANTC 3 cut(s) 384, 548, 742
HpaII CCGG 2 cut(s) 597, 617
HphI GGTGA 2 cut(s) 214, 440
Hpy166II GTNNAC 1 cut(s) 469
Hpy188I TCNGA 2 cut(s) 401, 741
Hpy188III TCNNGA 4 cut(s) 237, 363, 766, 800
Hpy8I GTNNAC 1 cut(s) 469
Hpy99I CGWCG 1 cut(s) 545
HpyAV CCTTC 3 cut(s) 285, 313, 662
HpyCH4III ACNGT 2 cut(s) 490, 605
HpyCH4IV ACGT 2 cut(s) 255, 573
HpyCH4V TGCA 4 cut(s) 370, 478, 688, 787
HpyF10VI GCNNNNNNNGC 4 cut(s) 214, 280, 715, 793
HpyF3I CTNAG 2 cut(s) 284, 780
HpySE526I ACGT 2 cut(s) 255, 573
Hsp92I GRCGYC 3 cut(s) 255, 300, 822
Hsp92II CATG 3 cut(s) 337, 803, 855
HspAI GCGC 1 cut(s) 196
Kzo9I GATC 1 cut(s) 613
LmnI GCTCC 2 cut(s) 193, 293
Lsp1109I GCAGC 3 cut(s) 490, 760, 763
LweI GCATC 4 cut(s) 255, 465, 583, 665
MaeI CTAG 1 cut(s) 165
MaeII ACGT 2 cut(s) 255, 573
MaeIII GTNAC 3 cut(s) 428, 447, 599
MalI GATC 1 cut(s) 615
MboI GATC 1 cut(s) 613
MboII GAAGA 4 cut(s) 340, 509, 529, 834
MhlI GDGCHC 2 cut(s) 290, 838
MlsI TGGCCA 1 cut(s) 524
MluNI TGGCCA 1 cut(s) 524
MlyI GAGTC 3 cut(s) 393, 542, 736
Mox20I TGGCCA 1 cut(s) 524
MscI TGGCCA 1 cut(s) 524
MseI TTAA 3 cut(s) 159, 246, 492
MslI CAYNNNNRTG 1 cut(s) 464
Msp20I TGGCCA 1 cut(s) 524
MspI CCGG 2 cut(s) 597, 617
MspR9I CCNGG 1 cut(s) 752
MvaI CCWGG 1 cut(s) 752
MvnI CGCG 2 cut(s) 198, 389
MwoI GCNNNNNNNGC 4 cut(s) 214, 280, 715, 793
NdeII GATC 1 cut(s) 613
NlaIII CATG 3 cut(s) 337, 803, 855
NlaIV GGNNCC 1 cut(s) 633
NmuCI GTSAC 2 cut(s) 428, 447
OliI CACNNNNGTG 1 cut(s) 464
PaeR7I CTCGAG 2 cut(s) 293, 374
PagI TCATGA 1 cut(s) 799
PkrI GCNGC 6 cut(s) 106, 130, 320, 480, 775, 778
PleI GAGTC 3 cut(s) 392, 542, 736
PpsI GAGTC 3 cut(s) 392, 542, 736
PpuMI RGGWCCY 1 cut(s) 631
PshBI ATTAAT 1 cut(s) 159
Psp124BI GAGCTC 1 cut(s) 290
Psp5II RGGWCCY 1 cut(s) 631
Psp6I CCWGG 1 cut(s) 750
PspGI CCWGG 1 cut(s) 750
PspN4I GGNNCC 1 cut(s) 633
PspPI GGNCC 2 cut(s) 469, 631
PspPPI RGGWCCY 1 cut(s) 631
PspXI VCTCGAGB 2 cut(s) 293, 374
RseI CAYNNNNRTG 1 cut(s) 464
SacI GAGCTC 1 cut(s) 290
SaqAI TTAA 3 cut(s) 159, 246, 492
SatI GCNGC 6 cut(s) 105, 129, 319, 479, 774, 777
Sau3AI GATC 1 cut(s) 613
Sau96I GGNCC 2 cut(s) 469, 631
SchI GAGTC 3 cut(s) 393, 542, 736
ScrFI CCNGG 1 cut(s) 752
SduI GDGCHC 2 cut(s) 290, 838
SfaNI GCATC 4 cut(s) 255, 465, 583, 665
Sfr274I CTCGAG 2 cut(s) 293, 374
SinI GGWCC 2 cut(s) 469, 631
SlaI CTCGAG 2 cut(s) 293, 374
SmiMI CAYNNNNRTG 1 cut(s) 464
SmlI CTYRAG 3 cut(s) 293, 374, 790
SmoI CTYRAG 3 cut(s) 293, 374, 790
SsiI CCGC 7 cut(s) 105, 126, 129, 318, 389, 437, 623
SspMI CTAG 1 cut(s) 165
SstI GAGCTC 1 cut(s) 290
StyD4I CCNGG 1 cut(s) 750
StyI CCWWGG 3 cut(s) 259, 555, 660
TaaI ACNGT 2 cut(s) 490, 605
TaiI ACGT 2 cut(s) 258, 576
TaqI TCGA 4 cut(s) 236, 294, 375, 382
TauI GCSGC 3 cut(s) 107, 131, 321
Tru1I TTAA 3 cut(s) 159, 246, 492
Tru9I TTAA 3 cut(s) 159, 246, 492
TscAI CASTG 2 cut(s) 154, 192
TseFI GTSAC 2 cut(s) 428, 447
TseI GCWGC 3 cut(s) 478, 773, 776
Tsp45I GTSAC 2 cut(s) 428, 447
TspDTI ATGAA 2 cut(s) 332, 788
TspGWI ACGGA 1 cut(s) 596
TspRI CASTG 2 cut(s) 154, 192
VpaK11BI GGWCC 2 cut(s) 469, 631
VspI ATTAAT 1 cut(s) 159
XhoI CTCGAG 2 cut(s) 293, 374
XspI CTAG 1 cut(s) 165
ZraI GACGTC 1 cut(s) 256
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.