Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0002019

Physical Location & Seq

Genomic Coordinates Forward (+)

164503 .. 169628

Gene Structure 5126 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1001300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1251 bp

ATGTTGGAGGGCGGTGCCAAATTCACTGGAATTATTGGTCTAAACAACCATGATAACAACTATGATGATTTGTCGCAAGGATTCTACCTCAAACTCCATGAGGGTGAGGGTACCAACATGTCCATCGACAGTATGCAAACGAGCAATGATGGAGGCTCTGTGGCCATGTCTATAGACAACAGCAGCGTTGCTTCAAATACTAATGATTCCCACACTCGAATCTTGAACCACCAAGGGCTGCGGCGACGTACTAAGGATAACTACTCCGTGCAACAGAGTGTTAACCACCGAGGAAGAGTCACACATGCTCTGAGTAATGATCAGCTTGCTCAAGCTCTACTTGACAGCCATTCCTCGACAGAAGGGCTTGAGAATTATGAAGAGTGGACAATTGACTTAAGAAAGCTAAACATGGGTGAAGCTTTTGCACAAGGTGCTTTTGGGAAGCTATATAGAGGTACCTACAATGGTGAAGATGTTGCTATCAAACTTTTGGAGAGTCCGGAAAATGACCCAGAAAAGGCTCAGGTGATGGAGCAGCAGTTTCAACAGGAAGTCAAGATGCTGGCTAATTTGAAGCATCCAAACATAGTTCGTTTCATCGGTGCTTGCCGTAAACCAATGGTTTGGTGCATTGTAACAGAGTATGCTAAGGGGGGTTCAGTTAGGCAGTTCTTGGCGAAGCGGCAGAGCCAATCAGTTCCATTGAAATTAGCAGTCAAGCAAGCATTAGATGTTGCAAGGGGGTTTGCTTATGTTCATGGGCTCGGTCTGATTCATAGGGACTTGAAATCTGACAACCTGTTGATTTCTTCTGACAAATCTATAAAAATTGCTGATTTTGGAGTTGCTCGTATTGAAGTGCAGACGGAAGGAATGACTCCGGAGACTGGGACATACCGCTGGATGGCGCCGGAGATGATCCAGCATAGGCATTACACACAGAAAGTTGACGTTTATAGCTTTGGGATTGTTCTTTGGGAACTTATAACGGGGATGCTTCCATTCCAGAACATGACAGCGGTACAGGCAGCATTTGCAGTTGTCAAGGGTGTCCGCCCTATCATTCCAAATGACTGCTTGCCTGTTCTTTGTGAGATCATGACGAGATGTTGGGATGCAAACCCTGAAGTCCGGCCATCCTTCACTGAAGTCGTGAGAATGCTCGAGCATGCAGAGATAGAAATCATGACGACTGTTCGCAAGGCCCGGTTCAGGTGTTGCATTACCCAGCCAATGACAGCGGACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0023052 GO:0035556 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase_Tyr

Protein Analysis

417

Amino Acids

46.69

Weight (kDa)

6.37

Isoelectric Point (pI)

38.67

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PK_Tyr_Ser-Thr	PF07714	137 - 389	3e-67	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	143 - 387	1.5e-55	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002019)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G24360 AT4G31170 AT4G31170 AT4G31170 AT4G31170 AT4G31170 AT4G31170 AT4G31170 AT4G31170 AT4G31170 AT4G31170
fragaria_vesca	FvH4_4g00150 FvH4_4g00150 FvH4_4g00150 FvH4_4g00150
malus_domestica	MD00G1091600.v1.1 MD04G1001200.v1.1 MD04G1001300.v1.1 MD13G1286000.v1.1
prunus_persica	Prupe.1G001200_v2.0.a1 Prupe.1G001300_v2.0.a1
pyrus_communis	pycom04g00180 pycom04g00190 pycom420g00210
rosa_chinensis	RchiOBHm_Chr3g0494021 RchiOBHm_Chr4g0384771
rosa_laevigata	RLG00000010273 RLG00000022687
rosa_multiflora	Rmu_sc0001322.1_g000022 Rmu_sc0003412.1_g000004
rosa_roxburghii	Rroxscaffold_5G00332830 Rroxscaffold_6G00391360
rosa_rugosa	Rorug03G0263700 Rorug03G0297500 Rorug03G0297500
rosa_samantha	Rh3AG313300 Rh3BG349400 Rh3CG346200 Rh3DG348700 Rh4AG001100 Rh4BG001000 Rh4CG000900 Rh4DG001000
rosa_wichuraiana	Rw3G027420 Rw4G000780

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	989
Acc65I	GGTACC	2 cut(s)	110, 458
AccB1I	GGYRCC	4 cut(s)	14, 110, 458, 910
AccIII	TCCGGA	2 cut(s)	502, 883
AciI	CCGC	7 cut(s)	12, 241, 685, 901, 1022, 1057, 1244
AclWI	GGATC	1 cut(s)	916
AcoI	YGGCCR	2 cut(s)	162, 1136
AcsI	RAATTY	1 cut(s)	20
AcuI	CTGAAG	2 cut(s)	1149, 1170
AcyI	GRCGYC	1 cut(s)	911
AdeI	CACNNNGTG	1 cut(s)	434
AfaI	GTAC	4 cut(s)	112, 250, 460, 1026
AfiI	CCNNNNNNNGG	4 cut(s)	520, 890, 907, 1215
AflII	CTTAAG	1 cut(s)	397
AflIII	ACRYGT	1 cut(s)	117
AgsI	TTSAA	7 cut(s)	195, 226, 548, 577, 709, 790, 860
AluBI	AGCT	6 cut(s)	325, 335, 406, 422, 448, 963
AluI	AGCT	6 cut(s)	325, 335, 406, 422, 448, 963
Alw26I	GTCTC	1 cut(s)	881
AlwI	GGATC	1 cut(s)	916
Ama87I	CYCGRG	1 cut(s)	1166
Aor13HI	TCCGGA	2 cut(s)	502, 883
AoxI	GGCC	3 cut(s)	162, 1136, 1206
ApeKI	GCWGC	4 cut(s)	183, 238, 538, 1031
ApoI	RAATTY	1 cut(s)	20
Asp718I	GGTACC	2 cut(s)	110, 458
AspLEI	GCGC	1 cut(s)	913
AspS9I	GGNCC	1 cut(s)	1207
AsuC2I	CCSGG	1 cut(s)	1210
AsuHPI	GGTGA	4 cut(s)	116, 428, 482, 541
AvaI	CYCGRG	1 cut(s)	1166
BalI	TGGCCA	1 cut(s)	164
BanI	GGYRCC	4 cut(s)	14, 110, 458, 910
BanII	GRGCYC	1 cut(s)	768
BbvI	GCAGC	4 cut(s)	195, 225, 550, 1043
BccI	CCATC	5 cut(s)	131, 143, 526, 901, 1147
BceAI	ACGGC	1 cut(s)	597
BclI	TGATCA	1 cut(s)	319
BcnI	CCSGG	1 cut(s)	1210
BcoDI	GTCTC	1 cut(s)	881
BfmI	CTRYAG	1 cut(s)	171
BfoI	RGCGCY	1 cut(s)	914
BfrI	CTTAAG	1 cut(s)	397
BisI	GCNGC	6 cut(s)	184, 239, 242, 539, 686, 1032
BlsI	GCNGC	6 cut(s)	185, 240, 243, 540, 687, 1033
Bme1390I	CCNGG	1 cut(s)	1210
BmeT110I	CYCGRG	1 cut(s)	1166
BmgT120I	GGNCC	1 cut(s)	1207
BmiI	GGNNCC	4 cut(s)	16, 112, 460, 912
BmrFI	CCNGG	1 cut(s)	1210
BmrI	ACTGGG	1 cut(s)	900
BmsI	GCATC	4 cut(s)	552, 589, 987, 1108
BmuI	ACTGGG	1 cut(s)	900
Bpu10I	CCTNAGC	2 cut(s)	525, 651
BpuEI	CTTGAG	2 cut(s)	315, 389
BpuMI	CCSGG	1 cut(s)	1210
BsaBI	GATNNNNATC	1 cut(s)	1184
BsaHI	GRCGYC	1 cut(s)	911
BsaJI	CCNNGG	2 cut(s)	232, 289
BsaWI	WCCGGW	2 cut(s)	502, 883
BsaXI	ACNNNNNCTCC	4 cut(s)	144, 174, 527, 557
Bsc4I	CCNNNNNNNGG	4 cut(s)	520, 890, 907, 1215
Bse1I	ACTGG	2 cut(s)	31, 895
Bse3DI	GCAATG	1 cut(s)	151
Bse8I	GATNNNNATC	1 cut(s)	1184
BseAI	TCCGGA	2 cut(s)	502, 883
BseDI	CCNNGG	2 cut(s)	232, 289
BseGI	GGATG	5 cut(s)	580, 912, 1002, 1123, 1139
BseJI	GATNNNNATC	1 cut(s)	1184
BseLI	CCNNNNNNNGG	4 cut(s)	520, 890, 907, 1215
BseMI	GCAATG	1 cut(s)	151
BseMII	CTCAG	2 cut(s)	302, 539
BseNI	ACTGG	2 cut(s)	31, 895
BseXI	GCAGC	4 cut(s)	195, 225, 550, 1043
BseYI	CCCAGC	1 cut(s)	1230
BsgI	GTGCAG	1 cut(s)	884
BshFI	GGCC	3 cut(s)	164, 1138, 1208
BshNI	GGYRCC	4 cut(s)	14, 110, 458, 910
BsiHKCI	CYCGRG	1 cut(s)	1166
BsiSI	CCGG	5 cut(s)	503, 884, 914, 1135, 1210
BslFI	GGGAC	2 cut(s)	797, 907
BslI	CCNNNNNNNGG	4 cut(s)	520, 890, 907, 1215
BsmAI	GTCTC	1 cut(s)	881
BsmFI	GGGAC	2 cut(s)	797, 907
BsmI	GAATGC	1 cut(s)	1167
BsnI	GGCC	3 cut(s)	164, 1138, 1208
BsoBI	CYCGRG	1 cut(s)	1166
Bsp1286I	GDGCHC	1 cut(s)	768
Bsp13I	TCCGGA	2 cut(s)	502, 883
Bsp143I	GATC	3 cut(s)	319, 921, 1098
BspACI	CCGC	7 cut(s)	12, 241, 685, 901, 1022, 1057, 1244
BspANI	GGCC	3 cut(s)	164, 1138, 1208
BspCNI	CTCAG	2 cut(s)	303, 538
BspEI	TCCGGA	2 cut(s)	502, 883
BspHI	TCATGA	2 cut(s)	1101, 1188
BspLI	GGNNCC	4 cut(s)	16, 112, 460, 912
BspPI	GGATC	1 cut(s)	916
BspT107I	GGYRCC	4 cut(s)	14, 110, 458, 910
BspTI	CTTAAG	1 cut(s)	397
BsrDI	GCAATG	1 cut(s)	151
BsrI	ACTGG	2 cut(s)	31, 895
BssECI	CCNNGG	2 cut(s)	232, 289
BssMI	GATC	3 cut(s)	319, 921, 1098
BssNI	GRCGYC	1 cut(s)	911
BssT1I	CCWWGG	1 cut(s)	232
Bst4CI	ACNGT	2 cut(s)	131, 1198
Bst6I	CTCTTC	2 cut(s)	289, 375
BstACI	GRCGYC	1 cut(s)	911
BstAFI	CTTAAG	1 cut(s)	397
BstAPI	GCANNNNNTGC	2 cut(s)	434, 1037
BstC8I	GCNNGC	6 cut(s)	327, 567, 610, 726, 1082, 1173
BstDEI	CTNAG	4 cut(s)	252, 311, 525, 651
BstF5I	GGATG	5 cut(s)	580, 912, 1002, 1123, 1139
BstH2I	RGCGCY	1 cut(s)	914
BstHHI	GCGC	1 cut(s)	913
BstKTI	GATC	3 cut(s)	322, 924, 1101
BstMAI	GTCTC	1 cut(s)	881
BstMBI	GATC	3 cut(s)	319, 921, 1098
BstMWI	GCNNNNNNNGC	3 cut(s)	434, 1028, 1037
BstNSI	RCATGY	3 cut(s)	121, 308, 1175
BstSCI	CCNGG	1 cut(s)	1208
BstSFI	CTRYAG	1 cut(s)	171
BstV1I	GCAGC	4 cut(s)	195, 225, 550, 1043
BstXI	CCANNNNNNTGG	1 cut(s)	627
BsuRI	GGCC	3 cut(s)	164, 1138, 1208
BtsCI	GGATG	5 cut(s)	580, 912, 1002, 1123, 1139
BtsIMutI	CAGTG	2 cut(s)	24, 1146
Cac8I	GCNNGC	6 cut(s)	327, 567, 610, 726, 1082, 1173
CciI	TCATGA	2 cut(s)	1101, 1188
CfoI	GCGC	1 cut(s)	913
Cfr13I	GGNCC	1 cut(s)	1207
Csp6I	GTAC	4 cut(s)	111, 249, 459, 1025
CviQI	GTAC	4 cut(s)	111, 249, 459, 1025
DdeI	CTNAG	4 cut(s)	252, 311, 525, 651
DinI	GGCGCC	1 cut(s)	912
DpnI	GATC	3 cut(s)	321, 923, 1100
DpnII	GATC	3 cut(s)	319, 921, 1098
DraIII	CACNNNGTG	1 cut(s)	434
EaeI	YGGCCR	2 cut(s)	162, 1136
Eam1104I	CTCTTC	2 cut(s)	289, 375
EarI	CTCTTC	2 cut(s)	289, 375
EciI	GGCGGA	1 cut(s)	1046
Eco130I	CCWWGG	1 cut(s)	232
Eco24I	GRGCYC	1 cut(s)	768
Eco57I	CTGAAG	2 cut(s)	1149, 1170
Eco88I	CYCGRG	1 cut(s)	1166
EcoT14I	CCWWGG	1 cut(s)	232
EcoT38I	GRGCYC	1 cut(s)	768
EgeI	GGCGCC	1 cut(s)	912
EheI	GGCGCC	1 cut(s)	912
ErhI	CCWWGG	1 cut(s)	232
FalI	AAGNNNNNCTT	2 cut(s)	324, 356
FaqI	GGGAC	2 cut(s)	797, 907
FbaI	TGATCA	1 cut(s)	319
Fnu4HI	GCNGC	6 cut(s)	184, 239, 242, 539, 686, 1032
FokI	GGATG	5 cut(s)	567, 919, 1009, 1126, 1130
FriOI	GRGCYC	1 cut(s)	768
Fsp4HI	GCNGC	6 cut(s)	184, 239, 242, 539, 686, 1032
GlaI	GCGC	1 cut(s)	912
GluI	GCNGC	6 cut(s)	184, 239, 242, 539, 686, 1032
GsaI	CCCAGC	1 cut(s)	1234
HaeII	RGCGCY	1 cut(s)	914
HaeIII	GGCC	3 cut(s)	164, 1138, 1208
HapII	CCGG	5 cut(s)	503, 884, 914, 1135, 1210
HhaI	GCGC	1 cut(s)	913
Hin1I	GRCGYC	1 cut(s)	911
Hin6I	GCGC	1 cut(s)	911
HinP1I	GCGC	1 cut(s)	911
HincII	GTYRAC	2 cut(s)	283, 952
HindII	GTYRAC	2 cut(s)	283, 952
HindIII	AAGCTT	1 cut(s)	420
HinfI	GANTC	7 cut(s)	81, 206, 219, 297, 499, 775, 880
HpaI	GTTAAC	1 cut(s)	283
HpaII	CCGG	5 cut(s)	503, 884, 914, 1135, 1210
HphI	GGTGA	4 cut(s)	116, 428, 482, 541
Hpy166II	GTNNAC	4 cut(s)	283, 387, 617, 952
Hpy188I	TCNGA	4 cut(s)	312, 774, 796, 817
Hpy188III	TCNNGA	8 cut(s)	223, 503, 559, 884, 1009, 1102, 1156, 1189
Hpy8I	GTNNAC	4 cut(s)	283, 387, 617, 952
Hpy99I	CGWCG	1 cut(s)	249
HpyAV	CCTTC	3 cut(s)	356, 866, 1153
HpyCH4III	ACNGT	2 cut(s)	131, 1198
HpyCH4IV	ACGT	2 cut(s)	247, 954
HpyF10VI	GCNNNNNNNGC	3 cut(s)	434, 1028, 1037
HpyF3I	CTNAG	4 cut(s)	252, 311, 525, 651
HpySE526I	ACGT	2 cut(s)	247, 954
Hsp92I	GRCGYC	1 cut(s)	911
HspAI	GCGC	1 cut(s)	911
KasI	GGCGCC	1 cut(s)	910
Kpn2I	TCCGGA	2 cut(s)	502, 883
KpnI	GGTACC	2 cut(s)	114, 462
Ksp22I	TGATCA	1 cut(s)	319
KspAI	GTTAAC	1 cut(s)	283
Kzo9I	GATC	3 cut(s)	319, 921, 1098
LmnI	GCTCC	1 cut(s)	535
Lsp1109I	GCAGC	4 cut(s)	195, 225, 550, 1043
LweI	GCATC	4 cut(s)	552, 589, 987, 1108
MaeII	ACGT	2 cut(s)	247, 954
MaeIII	GTNAC	2 cut(s)	298, 637
MalI	GATC	3 cut(s)	321, 923, 1100
MboI	GATC	3 cut(s)	319, 921, 1098
MboII	GAAGA	4 cut(s)	306, 392, 485, 804
MfeI	CAATTG	1 cut(s)	390
MhlI	GDGCHC	1 cut(s)	768
MlsI	TGGCCA	1 cut(s)	164
MluCI	AATT	7 cut(s)	20, 30, 373, 390, 571, 710, 831
MluNI	TGGCCA	1 cut(s)	164
Mly113I	GGCGCC	1 cut(s)	911
MlyI	GAGTC	3 cut(s)	306, 508, 874
MnlI	CCTC	7 cut(s)	94, 98, 100, 146, 284, 364, 449
Mox20I	TGGCCA	1 cut(s)	164
MroI	TCCGGA	2 cut(s)	502, 883
MscI	TGGCCA	1 cut(s)	164
MseI	TTAA	2 cut(s)	282, 398
MslI	CAYNNNNRTG	1 cut(s)	102
Msp20I	TGGCCA	1 cut(s)	164
MspA1I	CMGCKG	3 cut(s)	903, 1022, 1244
MspCI	CTTAAG	1 cut(s)	397
MspI	CCGG	5 cut(s)	503, 884, 914, 1135, 1210
MspR9I	CCNGG	1 cut(s)	1210
MunI	CAATTG	1 cut(s)	390
Mva1269I	GAATGC	1 cut(s)	1167
MwoI	GCNNNNNNNGC	3 cut(s)	434, 1028, 1037
NarI	GGCGCC	1 cut(s)	911
NciI	CCSGG	1 cut(s)	1210
NdeII	GATC	3 cut(s)	319, 921, 1098
NlaIV	GGNNCC	4 cut(s)	16, 112, 460, 912
NmuCI	GTSAC	1 cut(s)	298
NspI	RCATGY	3 cut(s)	121, 308, 1175
PaeI	GCATGC	1 cut(s)	1175
PaeR7I	CTCGAG	1 cut(s)	1166
PagI	TCATGA	2 cut(s)	1101, 1188
PciI	ACATGT	1 cut(s)	117
PctI	GAATGC	1 cut(s)	1167
PfeI	GAWTC	4 cut(s)	81, 206, 219, 775
PkrI	GCNGC	6 cut(s)	185, 240, 243, 540, 687, 1033
PleI	GAGTC	3 cut(s)	305, 507, 874
PluTI	GGCGCC	1 cut(s)	914
PpsI	GAGTC	3 cut(s)	305, 507, 874
PscI	ACATGT	1 cut(s)	117
PsiI	TTATAA	1 cut(s)	989
PspFI	CCCAGC	1 cut(s)	1230
PspN4I	GGNNCC	4 cut(s)	16, 112, 460, 912
PspPI	GGNCC	1 cut(s)	1207
PspXI	VCTCGAGB	1 cut(s)	1166
RsaI	GTAC	4 cut(s)	112, 250, 460, 1026
RsaNI	GTAC	4 cut(s)	111, 249, 459, 1025
RseI	CAYNNNNRTG	1 cut(s)	102
SaqAI	TTAA	2 cut(s)	282, 398
SatI	GCNGC	6 cut(s)	184, 239, 242, 539, 686, 1032
Sau3AI	GATC	3 cut(s)	319, 921, 1098
Sau96I	GGNCC	1 cut(s)	1207
SchI	GAGTC	3 cut(s)	306, 508, 874
ScrFI	CCNGG	1 cut(s)	1210
SduI	GDGCHC	1 cut(s)	768
SfaNI	GCATC	4 cut(s)	552, 589, 987, 1108
SfcI	CTRYAG	1 cut(s)	171
SfoI	GGCGCC	1 cut(s)	912
Sfr274I	CTCGAG	1 cut(s)	1166
SlaI	CTCGAG	1 cut(s)	1166
SmiMI	CAYNNNNRTG	1 cut(s)	102
SmlI	CTYRAG	4 cut(s)	330, 368, 397, 1166
SmoI	CTYRAG	4 cut(s)	330, 368, 397, 1166
SphI	GCATGC	1 cut(s)	1175
Sse9I	AATT	7 cut(s)	20, 30, 373, 390, 571, 710, 831
SsiI	CCGC	7 cut(s)	12, 241, 685, 901, 1022, 1057, 1244
SspDI	GGCGCC	1 cut(s)	910
StyD4I	CCNGG	1 cut(s)	1208
StyI	CCWWGG	1 cut(s)	232
TaaI	ACNGT	2 cut(s)	131, 1198
TaiI	ACGT	2 cut(s)	250, 957
TaqI	TCGA	4 cut(s)	126, 217, 356, 1167
TaqII	GACCGA	1 cut(s)	758
TasI	AATT	7 cut(s)	20, 30, 373, 390, 571, 710, 831
TauI	GCSGC	2 cut(s)	244, 688
TfiI	GAWTC	4 cut(s)	81, 206, 219, 775
Tru1I	TTAA	2 cut(s)	282, 398
Tru9I	TTAA	2 cut(s)	282, 398
TscAI	CASTG	2 cut(s)	31, 1153
TseFI	GTSAC	1 cut(s)	298
TseI	GCWGC	4 cut(s)	183, 238, 538, 1031
Tsp45I	GTSAC	1 cut(s)	298
TspDTI	ATGAA	4 cut(s)	393, 589, 749, 767
TspGWI	ACGGA	2 cut(s)	256, 884
TspRI	CASTG	2 cut(s)	31, 1153
Vha464I	CTTAAG	1 cut(s)	397
XapI	RAATTY	1 cut(s)	20
XceI	RCATGY	3 cut(s)	121, 308, 1175
XhoI	CTCGAG	1 cut(s)	1166

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.