Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4D

Orthogroup ID

OG0002019

Physical Location & Seq

Genomic Coordinates Reverse (-)

233074 .. 237642

Gene Structure 4569 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4DG001000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1251 bp

ATGTTGGAAGGTGGTGCCAAGTTCACTGGAATCATAGGTCTAAACAACAACGATAACAACTACTATGATTTGTCTCAAGTCTTTTACCACAAACTCCATGAGGGTGAGGGTACTAACATGTCCATTGACAGTATGCAAACTAGCGTTGATGGAGGCTCTGTCGCCATGTCTATAGATAACAGCAGCGTTGCTTCTAATACTAATGATTCCCACACACGCATCTTGAACCACCAAGGCCTGCGCCGACGCACAAATGACAACTACTCTGTTGCCCAAAGTGTTAATCGCGGAAGAGTCACCCACGCTCTCAGTGATGATCAGCTTGCCTGTGCTCTAATGGACAGCAATTCCGTCACAGAGGGGCTTGAGAATTATGAGGCGTGGACAATTGACTTAAGAAAGCTAAACATGGGTGACGCTTTTGCACAAGGTGCTTTTGGGAAGCTATACAGAGGTACTTACAACGGAGAGGATGTTGCTATCAAAATTTTGGAGAGGCCTGAGAATGACCCAGAAAAGGCTCAGATAATGGAGCAACAGTTTCAACAGGAAGTCAAGATGTTGGCTACGTTGAAGCATCCAAACATAGTTCGTTTTATTGGTGGCTGCCGTAAACCAATGGTTTGGTGCATTGTAACAGAGTATGCTAAGGGTGGTTCAGTACGGCAATTCTTGATGAGGCGGCAGAGCCGATCAGTACCGTTGAAGTTAGCAGTCAAGCAAGCTCTGGATGTTGCAAGGGGGATGGCATATGTTCATGGTCTCGGCCTGATTCACAGGGACTTGAAATCTGACAACCTTTTGATTTTTTCAGACAAATCAATTAAAATAGCTGATTTTGGGGTTGCCCGTATTGAGGTGCAGACGGAAGGAATGACACCGGAGACAGGGACATACCGCTGGATGGCTCCGGAGATGATCCAGCATCGGCCGTACACTCAGAAAGTTGATGTTTACAGCTTTGGGATTGTTCTTTGGGAACTTATTACAGGGATGCTTCCGTTCTCGAAAATGACAGCAGTGCAGGCAGCGTTTGCAGTTGTCAATAAGGGTGTCCGTCCTATCATTCCCGCTGACTGCTTAACTGTTCTTGGTGAGATAATGACGAGATGCTGGGATCCCAACCCTGATGCCAGGCCACCCTTCACCGAGGTGGTAAGAATGCTCGAGATTGCAGAGACAGAGATCATGACAACCGTTCGGAAGGCTCGTTTCAGGTGTTGCATGACCCAACCAATGACAGCCGACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0023052 GO:0035556 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase_Tyr

Protein Analysis

416

Amino Acids

46.62

Weight (kDa)

6.77

Isoelectric Point (pI)

37.78

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PK_Tyr_Ser-Thr	PF07714	136 - 389	8.1e-65	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	143 - 387	9.5e-53	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002019)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G24360 AT4G31170 AT4G31170 AT4G31170 AT4G31170 AT4G31170 AT4G31170 AT4G31170 AT4G31170 AT4G31170 AT4G31170
fragaria_vesca	FvH4_4g00150 FvH4_4g00150 FvH4_4g00150 FvH4_4g00150
malus_domestica	MD00G1091600.v1.1 MD04G1001200.v1.1 MD04G1001300.v1.1 MD13G1286000.v1.1
prunus_persica	Prupe.1G001200_v2.0.a1 Prupe.1G001300_v2.0.a1
pyrus_communis	pycom04g00180 pycom04g00190 pycom420g00210
rosa_chinensis	RchiOBHm_Chr3g0494021 RchiOBHm_Chr4g0384771
rosa_laevigata	RLG00000010273 RLG00000022687
rosa_multiflora	Rmu_sc0001322.1_g000022 Rmu_sc0003412.1_g000004
rosa_roxburghii	Rroxscaffold_5G00332830 Rroxscaffold_6G00391360
rosa_rugosa	Rorug03G0263700 Rorug03G0297500 Rorug03G0297500
rosa_samantha	Rh3AG313300 Rh3BG349400 Rh3CG346200 Rh3DG348700 Rh4AG001100 Rh4BG001000 Rh4CG000900 Rh4DG001000
rosa_wichuraiana	Rw3G027420 Rw4G000780

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	14
AccII	CGCG	1 cut(s)	288
AccIII	TCCGGA	1 cut(s)	910
AciI	CCGC	4 cut(s)	288, 682, 898, 1071
AclWI	GGATC	3 cut(s)	913, 1112, 1125
AcoI	YGGCCR	1 cut(s)	929
AcsI	RAATTY	1 cut(s)	486
AdeI	CACNNNGTG	1 cut(s)	431
AfaI	GTAC	5 cut(s)	112, 457, 663, 699, 935
AfiI	CCNNNNNNNGG	4 cut(s)	517, 856, 887, 904
AflII	CTTAAG	1 cut(s)	394
AflIII	ACRYGT	1 cut(s)	117
AgsI	TTSAA	5 cut(s)	226, 545, 574, 706, 787
AjnI	CCWGG	1 cut(s)	1133
AleI	CACNNNNGTG	1 cut(s)	1151
AluBI	AGCT	6 cut(s)	322, 403, 445, 725, 833, 960
AluI	AGCT	6 cut(s)	322, 403, 445, 725, 833, 960
Alw21I	GWGCWC	1 cut(s)	334
Alw26I	GTCTC	4 cut(s)	78, 767, 878, 1172
AlwI	GGATC	3 cut(s)	913, 1112, 1125
Ama87I	CYCGRG	1 cut(s)	1166
Aor13HI	TCCGGA	1 cut(s)	910
AoxI	GGCC	5 cut(s)	235, 497, 766, 929, 1136
ApeKI	GCWGC	3 cut(s)	183, 606, 1028
ApoI	RAATTY	1 cut(s)	486
AspLEI	GCGC	1 cut(s)	243
AsuHPI	GGTGA	5 cut(s)	116, 289, 425, 1106, 1138
AvaI	CYCGRG	1 cut(s)	1166
BamHI	GGATCC	1 cut(s)	1117
BanI	GGYRCC	1 cut(s)	14
Bbv12I	GWGCWC	1 cut(s)	334
BbvI	GCAGC	3 cut(s)	195, 593, 1040
BccI	CCATC	3 cut(s)	143, 739, 898
BceAI	ACGGC	3 cut(s)	594, 680, 916
BciT130I	CCWGG	1 cut(s)	1135
BclI	TGATCA	1 cut(s)	316
BcoDI	GTCTC	4 cut(s)	78, 767, 878, 1172
BfaI	CTAG	1 cut(s)	141
BfmI	CTRYAG	1 cut(s)	171
BfrI	CTTAAG	1 cut(s)	394
BisI	GCNGC	4 cut(s)	184, 607, 683, 1029
BlsI	GCNGC	4 cut(s)	185, 608, 684, 1030
Bme1390I	CCNGG	1 cut(s)	1135
BmeT110I	CYCGRG	1 cut(s)	1166
BmiI	GGNNCC	3 cut(s)	16, 909, 1119
BmrFI	CCNGG	1 cut(s)	1135
BmsI	GCATC	6 cut(s)	228, 586, 934, 984, 1100, 1120
Bpu10I	CCTNAGC	1 cut(s)	648
BpuEI	CTTGAG	2 cut(s)	60, 386
BsaI	GGTCTC	1 cut(s)	767
BsaJI	CCNNGG	2 cut(s)	232, 1149
BsaWI	WCCGGW	2 cut(s)	880, 910
BsaXI	ACNNNNNCTCC	6 cut(s)	144, 174, 459, 489, 524, 554
Bsc4I	CCNNNNNNNGG	4 cut(s)	517, 856, 887, 904
Bse1I	ACTGG	1 cut(s)	31
BseAI	TCCGGA	1 cut(s)	910
BseBI	CCWGG	1 cut(s)	1135
BseDI	CCNNGG	2 cut(s)	232, 1149
BseGI	GGATG	6 cut(s)	478, 577, 736, 750, 909, 999
BseLI	CCNNNNNNNGG	4 cut(s)	517, 856, 887, 904
BseMII	CTCAG	4 cut(s)	322, 492, 536, 953
BseNI	ACTGG	1 cut(s)	31
BseX3I	CGGCCG	1 cut(s)	929
BseXI	GCAGC	3 cut(s)	195, 593, 1040
BseYI	CCCAGC	1 cut(s)	1113
BsgI	GTGCAG	2 cut(s)	881, 1043
Bsh1236I	CGCG	1 cut(s)	288
Bsh1285I	CGRYCG	1 cut(s)	932
BshFI	GGCC	5 cut(s)	237, 499, 768, 931, 1138
BshNI	GGYRCC	1 cut(s)	14
BsiEI	CGRYCG	1 cut(s)	932
BsiHKAI	GWGCWC	1 cut(s)	334
BsiHKCI	CYCGRG	1 cut(s)	1166
BsiSI	CCGG	2 cut(s)	881, 911
BslFI	GGGAC	2 cut(s)	794, 904
BslI	CCNNNNNNNGG	4 cut(s)	517, 856, 887, 904
BsmAI	GTCTC	4 cut(s)	78, 767, 878, 1172
BsmFI	GGGAC	2 cut(s)	794, 904
BsmI	GAATGC	1 cut(s)	1167
BsnI	GGCC	5 cut(s)	237, 499, 768, 931, 1138
Bso31I	GGTCTC	1 cut(s)	767
BsoBI	CYCGRG	1 cut(s)	1166
Bsp1286I	GDGCHC	1 cut(s)	334
Bsp13I	TCCGGA	1 cut(s)	910
Bsp143I	GATC	5 cut(s)	316, 692, 918, 1117, 1185
BspACI	CCGC	4 cut(s)	288, 682, 898, 1071
BspANI	GGCC	5 cut(s)	237, 499, 768, 931, 1138
BspCNI	CTCAG	4 cut(s)	321, 493, 535, 952
BspEI	TCCGGA	1 cut(s)	910
BspFNI	CGCG	1 cut(s)	288
BspHI	TCATGA	1 cut(s)	1188
BspLI	GGNNCC	3 cut(s)	16, 909, 1119
BspPI	GGATC	3 cut(s)	913, 1112, 1125
BspT107I	GGYRCC	1 cut(s)	14
BspTI	CTTAAG	1 cut(s)	394
BspTNI	GGTCTC	1 cut(s)	767
BsrI	ACTGG	1 cut(s)	31
BssECI	CCNNGG	2 cut(s)	232, 1149
BssMI	GATC	5 cut(s)	316, 692, 918, 1117, 1185
BssT1I	CCWWGG	1 cut(s)	232
Bst2UI	CCWGG	1 cut(s)	1135
Bst4CI	ACNGT	5 cut(s)	131, 540, 702, 1087, 1198
Bst6I	CTCTTC	1 cut(s)	286
BstAFI	CTTAAG	1 cut(s)	394
BstAPI	GCANNNNNTGC	2 cut(s)	431, 1034
BstC8I	GCNNGC	4 cut(s)	239, 324, 723, 1026
BstDEI	CTNAG	5 cut(s)	308, 501, 522, 648, 939
BstF5I	GGATG	6 cut(s)	478, 577, 736, 750, 909, 999
BstFNI	CGCG	1 cut(s)	288
BstHHI	GCGC	1 cut(s)	243
BstKTI	GATC	5 cut(s)	319, 695, 921, 1120, 1188
BstMAI	GTCTC	4 cut(s)	78, 767, 878, 1172
BstMBI	GATC	5 cut(s)	316, 692, 918, 1117, 1185
BstMCI	CGRYCG	1 cut(s)	932
BstMWI	GCNNNNNNNGC	3 cut(s)	431, 1025, 1034
BstNI	CCWGG	1 cut(s)	1135
BstNSI	RCATGY	1 cut(s)	121
BstSCI	CCNGG	1 cut(s)	1133
BstSFI	CTRYAG	1 cut(s)	171
BstUI	CGCG	1 cut(s)	288
BstV1I	GCAGC	3 cut(s)	195, 593, 1040
BstX2I	RGATCY	1 cut(s)	1117
BstXI	CCANNNNNNTGG	1 cut(s)	624
BstYI	RGATCY	1 cut(s)	1117
BstZI	CGGCCG	1 cut(s)	929
BsuRI	GGCC	5 cut(s)	237, 499, 768, 931, 1138
BtsCI	GGATG	6 cut(s)	478, 577, 736, 750, 909, 999
BtsI	GCAGTG	1 cut(s)	1026
BtsIMutI	CAGTG	3 cut(s)	24, 316, 1026
Cac8I	GCNNGC	4 cut(s)	239, 324, 723, 1026
CciI	TCATGA	1 cut(s)	1188
CfoI	GCGC	1 cut(s)	243
CseI	GACGC	2 cut(s)	255, 425
Csp6I	GTAC	5 cut(s)	111, 456, 662, 698, 934
CviAII	CATG	7 cut(s)	98, 118, 166, 409, 758, 1189, 1225
CviQI	GTAC	5 cut(s)	111, 456, 662, 698, 934
DdeI	CTNAG	5 cut(s)	308, 501, 522, 648, 939
DpnI	GATC	5 cut(s)	318, 694, 920, 1119, 1187
DpnII	GATC	5 cut(s)	316, 692, 918, 1117, 1185
DraIII	CACNNNGTG	1 cut(s)	431
EaeI	YGGCCR	1 cut(s)	929
EagI	CGGCCG	1 cut(s)	929
Eam1104I	CTCTTC	1 cut(s)	286
EarI	CTCTTC	1 cut(s)	286
EclXI	CGGCCG	1 cut(s)	929
Eco130I	CCWWGG	1 cut(s)	232
Eco147I	AGGCCT	2 cut(s)	237, 499
Eco31I	GGTCTC	1 cut(s)	767
Eco52I	CGGCCG	1 cut(s)	929
Eco88I	CYCGRG	1 cut(s)	1166
EcoRII	CCWGG	1 cut(s)	1133
EcoT14I	CCWWGG	1 cut(s)	232
ErhI	CCWWGG	1 cut(s)	232
FaeI	CATG	7 cut(s)	101, 121, 169, 412, 761, 1192, 1228
FaqI	GGGAC	2 cut(s)	794, 904
FatI	CATG	7 cut(s)	97, 117, 165, 408, 757, 1188, 1224
FauI	CCCGC	1 cut(s)	1078
FauNDI	CATATG	1 cut(s)	751
FbaI	TGATCA	1 cut(s)	316
Fnu4HI	GCNGC	4 cut(s)	184, 607, 683, 1029
FokI	GGATG	6 cut(s)	485, 564, 743, 757, 916, 1006
Fsp4HI	GCNGC	4 cut(s)	184, 607, 683, 1029
FspBI	CTAG	1 cut(s)	141
GlaI	GCGC	1 cut(s)	242
GluI	GCNGC	4 cut(s)	184, 607, 683, 1029
GsaI	CCCAGC	1 cut(s)	1117
HaeIII	GGCC	5 cut(s)	237, 499, 768, 931, 1138
HapII	CCGG	2 cut(s)	881, 911
HgaI	GACGC	2 cut(s)	255, 425
HhaI	GCGC	1 cut(s)	243
Hin1II	CATG	7 cut(s)	101, 121, 169, 412, 761, 1192, 1228
Hin6I	GCGC	1 cut(s)	241
HinP1I	GCGC	1 cut(s)	241
HinfI	GANTC	4 cut(s)	30, 206, 294, 772
HpaII	CCGG	2 cut(s)	881, 911
HphI	GGTGA	5 cut(s)	116, 289, 425, 1106, 1138
Hpy166II	GTNNAC	5 cut(s)	24, 384, 614, 936, 955
Hpy188I	TCNGA	5 cut(s)	525, 793, 814, 942, 1203
Hpy188III	TCNNGA	8 cut(s)	223, 556, 673, 728, 911, 1006, 1168, 1189
Hpy8I	GTNNAC	5 cut(s)	24, 384, 614, 936, 955
Hpy99I	CGWCG	1 cut(s)	249
HpyAV	CCTTC	3 cut(s)	863, 1153, 1198
HpyCH4III	ACNGT	5 cut(s)	131, 540, 702, 1087, 1198
HpyCH4IV	ACGT	1 cut(s)	569
HpyCH4V	TGCA	9 cut(s)	136, 425, 630, 737, 862, 1024, 1037, 1175, 1224
HpyF10VI	GCNNNNNNNGC	3 cut(s)	431, 1025, 1034
HpyF3I	CTNAG	5 cut(s)	308, 501, 522, 648, 939
HpySE526I	ACGT	1 cut(s)	569
Hsp92II	CATG	7 cut(s)	101, 121, 169, 412, 761, 1192, 1228
HspAI	GCGC	1 cut(s)	241
Kpn2I	TCCGGA	1 cut(s)	910
Ksp22I	TGATCA	1 cut(s)	316
Kzo9I	GATC	5 cut(s)	316, 692, 918, 1117, 1185
LmnI	GCTCC	2 cut(s)	532, 913
Lsp1109I	GCAGC	3 cut(s)	195, 593, 1040
LweI	GCATC	6 cut(s)	228, 586, 934, 984, 1100, 1120
MaeI	CTAG	1 cut(s)	141
MaeII	ACGT	1 cut(s)	569
MaeIII	GTNAC	4 cut(s)	295, 352, 413, 634
MalI	GATC	5 cut(s)	318, 694, 920, 1119, 1187
MboI	GATC	5 cut(s)	316, 692, 918, 1117, 1185
MboII	GAAGA	1 cut(s)	303
MfeI	CAATTG	1 cut(s)	387
MflI	RGATCY	1 cut(s)	1117
MhlI	GDGCHC	1 cut(s)	334
MluCI	AATT	6 cut(s)	346, 370, 387, 486, 668, 822
MlyI	GAGTC	1 cut(s)	303
MroI	TCCGGA	1 cut(s)	910
MseI	TTAA	4 cut(s)	282, 395, 825, 1082
MslI	CAYNNNNRTG	2 cut(s)	102, 1151
MspA1I	CMGCKG	2 cut(s)	900, 1073
MspCI	CTTAAG	1 cut(s)	394
MspI	CCGG	2 cut(s)	881, 911
MspR9I	CCNGG	1 cut(s)	1135
MunI	CAATTG	1 cut(s)	387
Mva1269I	GAATGC	1 cut(s)	1167
MvaI	CCWGG	1 cut(s)	1135
MvnI	CGCG	1 cut(s)	288
MwoI	GCNNNNNNNGC	3 cut(s)	431, 1025, 1034
NdeI	CATATG	1 cut(s)	751
NdeII	GATC	5 cut(s)	316, 692, 918, 1117, 1185
NlaIII	CATG	7 cut(s)	101, 121, 169, 412, 761, 1192, 1228
NlaIV	GGNNCC	3 cut(s)	16, 909, 1119
NmeAIII	GCCGAG	1 cut(s)	744
NmuCI	GTSAC	3 cut(s)	295, 352, 413
NspI	RCATGY	1 cut(s)	121
OliI	CACNNNNGTG	1 cut(s)	1151
PaeR7I	CTCGAG	1 cut(s)	1166
PagI	TCATGA	1 cut(s)	1188
PceI	AGGCCT	2 cut(s)	237, 499
PciI	ACATGT	1 cut(s)	117
PcsI	WCGNNNNNNNCGW	1 cut(s)	1207
PctI	GAATGC	1 cut(s)	1167
PfeI	GAWTC	3 cut(s)	30, 206, 772
PkrI	GCNGC	4 cut(s)	185, 608, 684, 1030
PleI	GAGTC	1 cut(s)	302
PpsI	GAGTC	1 cut(s)	302
PscI	ACATGT	1 cut(s)	117
Psp6I	CCWGG	1 cut(s)	1133
PspFI	CCCAGC	1 cut(s)	1113
PspGI	CCWGG	1 cut(s)	1133
PspN4I	GGNNCC	3 cut(s)	16, 909, 1119
PsuI	RGATCY	1 cut(s)	1117
RsaI	GTAC	5 cut(s)	112, 457, 663, 699, 935
RsaNI	GTAC	5 cut(s)	111, 456, 662, 698, 934
RseI	CAYNNNNRTG	2 cut(s)	102, 1151
SaqAI	TTAA	4 cut(s)	282, 395, 825, 1082
SatI	GCNGC	4 cut(s)	184, 607, 683, 1029
Sau3AI	GATC	5 cut(s)	316, 692, 918, 1117, 1185
SchI	GAGTC	1 cut(s)	303
ScrFI	CCNGG	1 cut(s)	1135
SduI	GDGCHC	1 cut(s)	334
SfaNI	GCATC	6 cut(s)	228, 586, 934, 984, 1100, 1120
SfcI	CTRYAG	1 cut(s)	171
Sfr274I	CTCGAG	1 cut(s)	1166
SlaI	CTCGAG	1 cut(s)	1166
SmiMI	CAYNNNNRTG	2 cut(s)	102, 1151
SmlI	CTYRAG	4 cut(s)	75, 365, 394, 1166
SmoI	CTYRAG	4 cut(s)	75, 365, 394, 1166
Sse9I	AATT	6 cut(s)	346, 370, 387, 486, 668, 822
SseBI	AGGCCT	2 cut(s)	237, 499
SsiI	CCGC	4 cut(s)	288, 682, 898, 1071
SspMI	CTAG	1 cut(s)	141
StuI	AGGCCT	2 cut(s)	237, 499
StyD4I	CCNGG	1 cut(s)	1133
StyI	CCWWGG	1 cut(s)	232
TaaI	ACNGT	5 cut(s)	131, 540, 702, 1087, 1198
TaiI	ACGT	1 cut(s)	572
TaqI	TCGA	2 cut(s)	1007, 1167
TasI	AATT	6 cut(s)	346, 370, 387, 486, 668, 822
TauI	GCSGC	1 cut(s)	685
TfiI	GAWTC	3 cut(s)	30, 206, 772
Tru1I	TTAA	4 cut(s)	282, 395, 825, 1082
Tru9I	TTAA	4 cut(s)	282, 395, 825, 1082
TscAI	CASTG	3 cut(s)	31, 316, 1026
TseFI	GTSAC	3 cut(s)	295, 352, 413
TseI	GCWGC	3 cut(s)	183, 606, 1028
Tsp45I	GTSAC	3 cut(s)	295, 352, 413
TspDTI	ATGAA	1 cut(s)	746
TspGWI	ACGGA	5 cut(s)	340, 480, 881, 990, 1046
TspRI	CASTG	3 cut(s)	31, 316, 1026
Vha464I	CTTAAG	1 cut(s)	394
XapI	RAATTY	1 cut(s)	486
XceI	RCATGY	1 cut(s)	121
XhoI	CTCGAG	1 cut(s)	1166
XspI	CTAG	1 cut(s)	141

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.