MD04G1062800.v1.1

Agenet domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0002656
Physical Location & Seq
Forward (+)
8260047 .. 8273261
13215 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1062800.v1.1.491

Sequence Viewer

Length: 6918 bp
ATGGAGGGAGGTAGCGATTTCTCAAGGGATACACCCATGGATTATGATGACAATGATCTTCAAAGCCACAATGTTCATTTAGCTGGTGAAGGGAGTACTAATTGTCCTCCAGTTTTACAGCCATATGCTCTTCCCAAGTTTGATTTTGATGATAGCCTTCAAGGGCATTTGAGGTTTGATAGTTTGGTTGAAACCGAGGTTTTTCTGGGCATAGAGAGTAGCGAACCTAACCACTGGATTGAGGATTTCTCTCGTGGGAGTAGTGGGATAGAGTTTAGTTCCACTGCAGTAGAATCTTGCTCTATATCAAGGCGCAACAATGTTTGGTCTGAGGCGACTTCCTCAGAATCTGTTGAAATGCTATTAAAATCTGTTGGGCAGGAAGAAATTATCCCCTCTGAGACAATTTTTGAGGAATCAGATGCCTGTAAGGAACTGAATTGCTTAACTGAGCAGATGGAGCCTAACCTGGATAATGATCATAAGATTCTTTCTCAAACGGGGAATGTTACAGTTCTACAGCCTACATTACAGCAGGGGGAGACTTCCAAAAACCTTTCTAGCTTAAAGGATGTAGGAGTAGATCAGCTTTGTGCTGATGATTCACAAACCCGTGAAGGTAAATTATTAGTTGATGGAAATTCAAATAACTTGGAACCAAATGATCTTAGTGGAAAGGACACCCCGCATGTAAGTGAAGGGAGTCCTTTTGCTGATGGGAAATGCAAAGGTGTAAATCAGATGGAATTGGAATATGTGGTTGATGAACCTCCAGATAAAAAAGAAGATTCCTCTGCTTCAGGAATGCAAGTTGATTATATGATATCTGTGCAAAATATTAATACAAGTGATGAGTTGAGTAACAAAGATGTCCAAGATAATTTAAAAGATATCACTGAAGAGAATCCAGACGGTCATGTGTTGAGCATAGAGGCTCAACCTGTGAATGAAAAAGTAGTTGAAAAGGCTACCTGTTATTTGGAAAACCCTCATTGTTCATCGTTTGAGGTGGAGTCTGTGGAACGAGGAATTGCTAATCAAGAAAATGTTATTAGTGTGGAGGAACCGTTGGATATGATACTGCAAGAAGATTCTGACTTACATGCCCTGGGTGAATGCAGTGACAGGGAAATCTCTGGAGTTGATGCTGACACCAACAAATGTGAAAATGTGGTCTTGTTTAAAGGCATAGATACTGGTGGTGATCAATCAGAACCAACCACACAGAGCTTATCACCTAGGGCCTATGAAAATAAGACTAGCTGTGCAGTTGAGCCTAGCAACAAAAATGCTGAAATTTCTTCAAGTCTAGATCCTATACTGAAAGGGGATTCTGACTTACATATGCCGGATGGATTCGGTGACAGGGGGTGCTCTGAGGTTCCTGCGGTGACCAACAAATGTGAAGATGCAGTCTTGCTTAAAGATACGGGTACTGGTGGTGATCATTTTAAATTGAACACACATGATGTATCACCAATGGTTAACAGAGGCGATGATAGGTATGCAGTTGAGGTCACCAACAGCAATGCTGGTATTTCTTCAACTCTAGATCCTAAACTGAAGGTGGATTCTCAATGGAGTAGCTCCAAGGAGAATGCATTAGAAAGTGGTTTCCAACCAGATAGTGGGACTTTGGTCAGAACATCCGAGGCTTCGATTGAGGAAAATGATGTCTCTAAGGATGGAAGTAATGAAAATAGGGAAGTTCATTGTAACTTGACTGCAACATCTTCTTCAGCTGAAGTATTTAGTGAAGCATATGTAACTGGATCTTCTAAAAGTCCTCGTGATGCTTTTGGAATTTCTAGAGAGAAATTGAATGCTCACGGACATGTATCACTCTCTATTCTTGAGGAGTCAACTCAAATGTGTGATGATAATAAAGTTTACGGGGAAGGCGATGTTGGTGATGGACCAGATGGTAACCTGGATCTCCCTTCCAGTAAAAAGGATAGGATACAATTGCTCAATGATTCGAATGGTAAAGATTTGAAGTTTGATGGATCTGTTGTTAAGGGATCTGGATCCTCGCCATTGTGTGAAGGTAGCACAGATAAAGAGCTGATTGTTCCAATCCTAACACATGAAACTGATGGTAATGAACCAGCGGCCAACCTTTCCCTGGAAAATTCGAATTTGGCTTCTTGTGGCACCACGAATGCTGCTCCCTTGTCTTCAGGAAATGGTGTAGCCAGCGATATTGTTAGCCACTCAGAGGTTCAAGCAGCGCCTTCTTCTGCTGCAGGATCACACTGTGACAAAAAAGAAGAAACTGCCAACGAAATGACTAAAGATGCAAGTTTTTCATGTGTAGTGCCACCTCCTTCTGTAGAAACTGGGCTAGTTTCTGTTTCCGAAGCGGAAAAAGGTGTTTCTGGACAGTCGTTGTGCAAAACAGTTGATCAGTCTCTGCCTGTTACAGACAGTTGCAATAAAGAGTGCCAAAATGAACCACAAAATGCAGTTGTTAATGAAGTTAGCAAGAGAAACACAAATGAGAGGGAAGTTGCTTCAGATCAGTGTGAATCTTCTGCAAAAGAGGGTGGTGGTGCTGAAGCTATTGTCTTTGGAAAACAGGATGGCTCAACCATAAAAGAGAGTTCTGAAAGGGCATCTGCAAATGTAACAGAACCAATTATGGATTCTGACACGCGGACTGGGCCTTCAACATTGGTAGAAGTGTGTGGTGGTACTGCTAAAAATGTAATGAAAGACACTGACACTTCTGAAGTATCTCAGGATAAGACTTCTGCAGAGGCTATTATGCCCAGCATTAATTGTGACACTTCTATGATTTGTGAAGCTTCTATTTGTTCTGCTTCTTTGCCTGAATCTCATGCTAGGTTTGTTGCGCCAGAAAGTGATTTAAGTTCTGTTGGTCCAAACAAAACTGATTGTGGTCCAACAAAGGTTGTTGAAACAATTGAGCTTTCTGAGACTAAGCATGATTCAGGTGATATCAAGGGGCCGAAAATTCTGAGTGCCCCAATTTTGGACAGTGTTAGGGATGGTGACAATCATTCTCCTAATTCCTGGAATCCAAAAGGAAATGATGCCTCTAAGGATTTGAGAAATGGCACTTCGGATGTGAGTTTATTTACAGATTTGCCGAAAGGGGAAGCTGCCAACAACTTGCAGCCCGTTCCTGCTATTATACCTCCCAGATTAGCAGAGGTGTCTATTCAAAATTCTGGTTCAGGCCAGCTGGATGCAAAAATCTCACAAGTTGCACAGGGTGCTTCCAAGGGTACCCCTGAACGGAAAACAAGGCGAGCATCTAGCAAGGCAACAGGTAAACAGAGTGCTAAGAAGAGAAGTGTGCAAGCAAGAGGGGACAGATCGAGCAGTGTGTCCCAAAACCAATCTGGAATTTTCCAGCTTGTGCCACCTAGTGAGACAAAGGCCTATGGGCATGTGGATGGTTCTGTAAAACCCTTTTCTGTTCTTACTACTTCCACATCTAGTTTGCCAGATCTAAATACTTCTGCTCCACCATCTGTAATATTTCAACAGCCGTTCACTGACTTGCAGCAAGTGCAATTACGCGCTCAGATCTTTGTTTATGGTGCTTTAATTCAAGGAATAGCACCTGAAGAAGCTTATATGGTGTCAGCATTTGGGGGACATGATGGTGGAGGGGGCATGTGGGAGAATGCCTGGCGGGTGTCCATAGAGAGGCTACACAGCCAAAAGTCTAGTCCGGTTAATCCAGAAACCCCTTTGCAATCACGTTCTGACCTGAGGTTTACAGGTTCTAGGGCTCCTGACCAAGTAATTAAACATGGTGCATTCCAGAGTAAAAGTATTCCCTCACCTCTTGGTCGACCCAGCTCGAAGGGCACTCCACCAACGGGTAGCCCAATGATACCTATTTCATCACCGCTTTGGAGTATTTCTACCCCTGTTTGTGAGGGCCTGCAATATAATTATCAGCAGGCACTCAATCCATTGCATCCTTTCCAAACACCACCAATAGGGAACATATTTGGGGGCCATAATGCTACGTGGATTCCTCAGGCCTCCTTTCGGGGTCCCTGGCTTGCTTCTTCACAGTCTTCTGCAGCTCAAGCCAGTAACCTTTTTTCAGCATTTCCTAGTACAGAAGCAGTGCAGTTGACTCCTGTAAAAGAAACATCTCTGCCACAATTGCCTAGTATGAAGCATGTTTCCTCTGGTCCTTCTGCTCAGACTGGGGCCCCAACTAGTACTTTTGCAGGGGCGTCTGCAGTGTTTGACCCCAAAAAGGTGTTAGCATCACAGGGCCAGCATTCTGCTGACCCTAAGCCTAGAAAAAGAAAAAAGACTTCAGCTTCCAAGGATCATGTTCAGGTTACTATGCAAGCTCAATCTCAACCCGAGTCAGCTTTAACACTTGCTGGTAGTAGTAGTCTGTCTACATCTGTTGCCATCTCAACCCCAAGTACCATTGTGTCTAAGACCATGCCAGAGAAATTGATTATGTCTGTGTTTCAGATGCCTTCCACTGGTCACCTCAAAAAAGCAGATCAGGATTTGGAGCAGAGGGCAACTTTATCAGAGGAGACTCTTAGTAAAGTTAAGGAGGCCAGGCAACAGGCAGAAGAAGCTGCTGCTTATGCTGCTGCAGCTGTTAGTCACAGCCAAGAAATTTGGGATCATTCGGATAAGCAAAAAAATTCCAGATTGATATTAGACGTCGAAGCTAAGTTGGCATCTGCTGCTGCAGCTGTAGCTGCCGCGGCTGCTGTTGCGAAGGCAGCAGCTGCAGCTGCCAATGTTGCATCAAATGCTGCATTACAAGCAAAATTGATGGCTGAAGAAGCCTTGGTTTCATATAATGATGGGAATGCAAGCCAAAGTCCTGTGCCCATTTTAAGGGGTGAAGATGGAACAAATAGTTCAACTTCGATCCTTGTTGCAGCTAGGGAGGCTGTTAGAAGGAGAGTTGAAGTTGCATCTGCAGCCTCAAAGCGAGCTGAAAATATGGATGCTATTGTAAAAGCTGCTGAGCTGGCAGCAGAAGCTGTATCACAAGCTGGAATAATTGTTGCTATGGGTGATCCTTTGCCATTGAGTCAGTTAGTAGAAGCTGGTCCAGAGGGTTATTGGAAAGTACCTCAAGTTTCTTCAGGACTGGTTATGAAATCCAGTGGCATGTCAAGAGAACAGTCAAATTTAGTTACTGTTGGAGAAGATGCTGATACTTCTGCTAGGGGTTCTAAAGATAGACAATCAGATAAGCATGAAGCACAGCTGACTGCACATGAGAAGTCACCTATTCTGAGAGAGGTAAATAAGGAATCAATGGATGATCAATTGAGGCGGGGTGTAGGAACCACGGGGTTTGATACAGTCATTGAAAAGGGGTCAATAGGACCAAAAGGCCGCAATGTTTCTGAGATCATATCACACTCTGCTTTGATTACTGTCGAGAATGATTTTGAAAAGGAAGCAGAAGCATCTGAAGAAAGCAGCATCAAGGAGGGTTCTCTAGTAGAGGTTCTCAAAGATGGGGCTGGATTTGGAGAAGCCTGGTTTACTGCTAAGGTATTGTGTTTGCAGGATGGAAAAGCCTGTGTGTGTTACACTGAGCTTCAGTCAGATGAAGGGAAACTGCAGGAATGGGTGGCACTTGAAGGTGAAGAAGATAAGCCACCAAAGATACGAATTGCCCGCCCTGTTACTGCTTTGGGACACGACGGAACAAGGAAAAGGCGCAGAGCAGCGATGGCAGATTATACTTGGTCTGTTGGAGATAAAGTTGATGCATGGATACAGGAGAGCTGGTGGGAAGGAGTTGTCACTGAGAAAAACAAGAAAGATGAAACTATATTAACTGTCCACTTTCCAGCTCAAGGGGAAAAGTCAGTTGTTAAAGCTTGGCATCTTCGGCCTTCTCTCATTTGGAAGGATGGCAAATGGGTTGAATGGTTTAGTGTGCGAAATGACACCTCCTTCCTTGAGGGTGATATGCCTCAGGAAAAGCGACCCAAATTGGGCAGTCCTGCAGTGGAAGGCAAAGCGAAGGATAGCACATCAAAAGGCATTGATGTTGTTGACTCAGGGAAACCTGTAGACCCAAGGGTACTGAATTTATCTGCAAATGAAAAGGTATTTAATATGGGTACGAATACCAGAACTGAGAATAAGCTTGATGCAACCAGAACAATTCGAACAGGGTTGCAGAAGGAAGGATCAAGGGGGGTTGTTTTTGGTATTCCCAAGCCTGGAAAGAAGAGAAAATTCATGGAAGTTAGCAAACATTATGTGGAAAATGAGAGTAACAAGACTAATGAAACTAGTGATTCGGTGAAATTTCCGAAATACTTGATGCCTCAAGGATCCCGTGGATTGAAAAATACATCTAAAATTGACAAGAGGGAAAAACAAGTGGCAGAATCCAAGCTAAAGGGTCTGAGATCTGGAAAGCTGCAAAGTTTGTCAGGTAAATCTGCCGCACAGAAGGACAACCTCTTAACAGATGCACGCAGTGCCTCTGATGGTTCAAGTGAAATGGACAATACAGGAAAGATCAAAGATTCTGTAAACCGTGCCGAGGGTTTATCCGGAAAGCATACCCTATCACAAACAACACAGGGCCAAATTGAATTTTCTTCACTAGCTCCATCATCTGATTCTTCATTTAAGAAAGTCTCCACTTTAACTGCTAAATCTCGGGCAAACAAAGGAAAACTTGCACCTGCTGGTGGAAGGTTGGGTAAAATTGAGGAGGGCAAAGTGTTTAGCGGAAATCCAGCAAAGTCGACTGCTGAAGTTGTTGAGCCTCGTAGATCAAATCGCAGAATTCAACCTACATCAAGACTACTAGAAGGACTGCAAAGCTCGTTGATCATCTCAAAGATTCCTTCTGTTTCACATGACAAGGGCCACAGAAGTCAGAACCGGAATGCCTCTAGAGGGAATAACAATGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Agenet

Protein Analysis

2306

Amino Acids

246.36

Weight (kDa)

5.13

Isoelectric Point (pI)

47.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Agenet PF05641 1823 - 1883 3e-08 Agenet domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 6691
AatII GACGTC 1 cut(s) 4680
Acc36I ACCTGC 1 cut(s) 6691
Acc65I GGTACC 1 cut(s) 3251
AccB1I GGYRCC 2 cut(s) 2153, 3251
AccB7I CCANNNNNTGG 1 cut(s) 1628
AccI GTMKAC 4 cut(s) 3826, 4397, 6057, 6746
AccII CGCG 3 cut(s) 2656, 3549, 4721
AccIII TCCGGA 1 cut(s) 6548
AcoI YGGCCR 1 cut(s) 2112
AcyI GRCGYC 2 cut(s) 4223, 4677
AdeI CACNNNGTG 4 cut(s) 2258, 3239, 3395, 6473
AflIII ACRYGT 1 cut(s) 1834
AhlI ACTAGT 2 cut(s) 4205, 6281
AjuI GAANNNNNNNTTGG 4 cut(s) 1890, 1922, 2629, 2661
Alw21I GWGCWC 1 cut(s) 1376
Alw26I GTCTC 8 cut(s) 395, 536, 1681, 2415, 2933, 3392, 4538, 6640
AlwNI CAGNNNCTG 4 cut(s) 350, 2413, 4745, 5006
Ama87I CYCGRG 2 cut(s) 4358, 6657
Aor13HI TCCGGA 1 cut(s) 6548
ApaI GGGCCC 1 cut(s) 4201
ArsI GACNNNNNNTTYG 2 cut(s) 3685, 3717
AseI ATTAAT 2 cut(s) 840, 2778
Asp718I GGTACC 1 cut(s) 3251
AspA2I CCTAGG 1 cut(s) 1238
AspLEI GCGC 5 cut(s) 315, 2233, 2857, 3551, 5700
AsuII TTCGAA 3 cut(s) 1979, 2135, 6154
AvaI CYCGRG 2 cut(s) 4358, 6657
AvaII GGWCC 7 cut(s) 1916, 2882, 2903, 4034, 4178, 5075, 5357
AvrII CCTAGG 1 cut(s) 1238
AxyI CCTNAGG 3 cut(s) 3743, 4017, 5958
BaeGI GKGCMC 4 cut(s) 2989, 3845, 4201, 4851
BamHI GGATCC 2 cut(s) 2027, 6323
BanI GGYRCC 2 cut(s) 2153, 3251
BanII GRGCYC 2 cut(s) 3766, 4201
BauI CACGAG 2 cut(s) 252, 1788
BbsI GAAGAC 2 cut(s) 2169, 4050
Bbv12I GWGCWC 1 cut(s) 1376
BceAI ACGGC 1 cut(s) 3502
BciVI GTATCC 3 cut(s) 22, 1953, 5748
BclI TGATCA 6 cut(s) 478, 1204, 1444, 2404, 5293, 6831
BcoDI GTCTC 8 cut(s) 395, 536, 1681, 2415, 2933, 3392, 4538, 6640
BcuI ACTAGT 2 cut(s) 4205, 6281
BfoI RGCGCY 1 cut(s) 2234
BfuAI ACCTGC 1 cut(s) 6691
BfuI GTATCC 3 cut(s) 22, 1953, 5748
BglII AGATCT 3 cut(s) 3475, 3555, 6401
BlnI CCTAGG 1 cut(s) 1238
BlpI GCTNAGC 1 cut(s) 4989
BmcAI AGTACT 2 cut(s) 97, 4210
Bme18I GGWCC 7 cut(s) 1916, 2882, 2903, 4034, 4178, 5075, 5357
BmeT110I CYCGRG 2 cut(s) 4358, 6657
BmrI ACTGGG 3 cut(s) 2349, 2670, 4203
BmuI ACTGGG 3 cut(s) 2349, 2670, 4203
BoxI GACNNNNGTC 1 cut(s) 1637
BpiI GAAGAC 2 cut(s) 2169, 4050
BplI GAGNNNNNCTC 2 cut(s) 233, 265
BpmI CTGGAG 3 cut(s) 93, 756, 1158
Bpu10I CCTNAGC 2 cut(s) 4284, 5526
Bpu1102I GCTNAGC 1 cut(s) 4989
Bpu14I TTCGAA 3 cut(s) 1979, 2135, 6154
BpuEI CTTGAG 7 cut(s) 7, 1874, 4053, 5085, 5820, 5963, 6303
BsaAI YACGTR 1 cut(s) 4008
BsaBI GATNNNNATC 2 cut(s) 477, 2026
BsaHI GRCGYC 2 cut(s) 4223, 4677
BsaWI WCCGGW 3 cut(s) 3703, 6548, 6885
BsaXI ACNNNNNCTCC 8 cut(s) 1052, 1082, 3475, 3505, 5727, 5753, 5757, 5783
Bse21I CCTNAGG 3 cut(s) 3743, 4017, 5958
Bse3DI GCAATG 3 cut(s) 1534, 3950, 5377
Bse8I GATNNNNATC 2 cut(s) 477, 2026
BseAI TCCGGA 1 cut(s) 6548
BseJI GATNNNNATC 2 cut(s) 477, 2026
BseMI GCAATG 3 cut(s) 1534, 3950, 5377
BseRI GAGGAG 3 cut(s) 1871, 4556, 6725
BseSI GKGCMC 4 cut(s) 2989, 3845, 4201, 4851
BseYI CCCAGC 2 cut(s) 2771, 3830
BsgI GTGCAG 3 cut(s) 1287, 4133, 5226
Bsh1236I CGCG 3 cut(s) 2656, 3549, 4721
BshNI GGYRCC 2 cut(s) 2153, 3251
BsiHKAI GWGCWC 1 cut(s) 1376
BsiHKCI CYCGRG 2 cut(s) 4358, 6657
BsiSI CCGG 4 cut(s) 1349, 3704, 6549, 6886
BslFI GGGAC 6 cut(s) 1645, 3340, 3350, 3639, 4020, 5688
BsmAI GTCTC 8 cut(s) 395, 536, 1681, 2415, 2933, 3392, 4538, 6640
BsmFI GGGAC 6 cut(s) 1645, 3340, 3350, 3639, 4020, 5688
BsoBI CYCGRG 2 cut(s) 4358, 6657
Bsp119I TTCGAA 3 cut(s) 1979, 2135, 6154
Bsp120I GGGCCC 1 cut(s) 4197
Bsp1286I GDGCHC 6 cut(s) 1376, 2989, 3766, 3845, 4201, 4851
Bsp13I TCCGGA 1 cut(s) 6548
Bsp1720I GCTNAGC 1 cut(s) 4989
Bsp19I CCATGG 1 cut(s) 36
BspEI TCCGGA 1 cut(s) 6548
BspFNI CGCG 3 cut(s) 2656, 3549, 4721
BspMI ACCTGC 1 cut(s) 6691
BspQI GCTCTTC 1 cut(s) 135
BspT104I TTCGAA 3 cut(s) 1979, 2135, 6154
BspT107I GGYRCC 2 cut(s) 2153, 3251
BsrDI GCAATG 3 cut(s) 1534, 3950, 5377
BssNI GRCGYC 2 cut(s) 4223, 4677
BssSI CACGAG 2 cut(s) 252, 1788
BssT1I CCWWGG 7 cut(s) 36, 1238, 1590, 3246, 4317, 4806, 6062
Bst2BI CACGAG 2 cut(s) 252, 1788
Bst6I CTCTTC 4 cut(s) 135, 894, 3308, 6212
BstACI GRCGYC 2 cut(s) 4223, 4677
BstAPI GCANNNNNTGC 6 cut(s) 3239, 3538, 4700, 4745, 4769, 6473
BstBAI YACGTR 1 cut(s) 4008
BstBI TTCGAA 3 cut(s) 1979, 2135, 6154
BstDSI CCRYGG 4 cut(s) 36, 4719, 5319, 6328
BstEII GGTNACC 4 cut(s) 1390, 1516, 1925, 4490
BstFNI CGCG 3 cut(s) 2656, 3549, 4721
BstH2I RGCGCY 1 cut(s) 2234
BstHHI GCGC 5 cut(s) 315, 2233, 2857, 3551, 5700
BstMAI GTCTC 8 cut(s) 395, 536, 1681, 2415, 2933, 3392, 4538, 6640
BstNSI RCATGY 7 cut(s) 692, 1106, 1838, 3419, 3649, 4169, 5140
BstPAI GACNNNNGTC 1 cut(s) 1637
BstPI GGTNACC 4 cut(s) 1390, 1516, 1925, 4490
BstSLI GKGCMC 4 cut(s) 2989, 3845, 4201, 4851
BstUI CGCG 3 cut(s) 2656, 3549, 4721
BstV2I GAAGAC 2 cut(s) 2169, 4050
Bsu36I CCTNAGG 3 cut(s) 3743, 4017, 5958
BsuI GTATCC 3 cut(s) 22, 1953, 5748
BtgI CCRYGG 4 cut(s) 36, 4719, 5319, 6328
BtgZI GCGATG 3 cut(s) 1509, 1917, 5723
BtsI GCAGTG 7 cut(s) 282, 1126, 3355, 4116, 4236, 5997, 6478
BveI ACCTGC 1 cut(s) 6691
CaiI CAGNNNCTG 4 cut(s) 350, 2413, 4745, 5006
CfoI GCGC 5 cut(s) 315, 2233, 2857, 3551, 5700
Cfr42I CCGCGG 1 cut(s) 4722
CseI GACGC 1 cut(s) 4212
CspCI CAANNNNNGTGG 2 cut(s) 5308, 5343
DraI TTTAAA 3 cut(s) 885, 1183, 1453
DraIII CACNNNGTG 4 cut(s) 2258, 3239, 3395, 6473
EaeI YGGCCR 1 cut(s) 2112
Eam1104I CTCTTC 4 cut(s) 135, 894, 3308, 6212
EarI CTCTTC 4 cut(s) 135, 894, 3308, 6212
Eco130I CCWWGG 7 cut(s) 36, 1238, 1590, 3246, 4317, 4806, 6062
Eco147I AGGCCT 2 cut(s) 3407, 4022
Eco24I GRGCYC 2 cut(s) 3766, 4201
Eco32I GATATC 3 cut(s) 825, 892, 2962
Eco47I GGWCC 7 cut(s) 1916, 2882, 2903, 4034, 4178, 5075, 5357
Eco81I CCTNAGG 3 cut(s) 3743, 4017, 5958
Eco88I CYCGRG 2 cut(s) 4358, 6657
Eco91I GGTNACC 4 cut(s) 1390, 1516, 1925, 4490
EcoO109I RGGNCCY 5 cut(s) 1242, 3916, 4034, 4197, 4198
EcoO65I GGTNACC 4 cut(s) 1390, 1516, 1925, 4490
EcoRI GAATTC 1 cut(s) 6786
EcoRV GATATC 3 cut(s) 825, 892, 2962
EcoT14I CCWWGG 7 cut(s) 36, 1238, 1590, 3246, 4317, 4806, 6062
EcoT22I ATGCAT 2 cut(s) 1603, 5752
EcoT38I GRGCYC 2 cut(s) 3766, 4201
ErhI CCWWGG 7 cut(s) 36, 1238, 1590, 3246, 4317, 4806, 6062
FalI AAGNNNNNCTT 2 cut(s) 6660, 6692
FaqI GGGAC 6 cut(s) 1645, 3340, 3350, 3639, 4020, 5688
FauI CCCGC 4 cut(s) 693, 3657, 5298, 5663
FauNDI CATATG 3 cut(s) 124, 1344, 1762
FbaI TGATCA 6 cut(s) 478, 1204, 1444, 2404, 5293, 6831
FblI GTMKAC 4 cut(s) 3826, 4397, 6057, 6746
FriOI GRGCYC 2 cut(s) 3766, 4201
GlaI GCGC 5 cut(s) 314, 2232, 2856, 3550, 5699
GsaI CCCAGC 2 cut(s) 2775, 3834
GsuI CTGGAG 3 cut(s) 93, 756, 1158
HaeII RGCGCY 1 cut(s) 2234
HapII CCGG 4 cut(s) 1349, 3704, 6549, 6886
HgaI GACGC 1 cut(s) 4212
HhaI GCGC 5 cut(s) 315, 2233, 2857, 3551, 5700
Hin1I GRCGYC 2 cut(s) 4223, 4677
Hin6I GCGC 5 cut(s) 313, 2231, 2855, 3549, 5698
HinP1I GCGC 5 cut(s) 313, 2231, 2855, 3549, 5698
HincII GTYRAC 6 cut(s) 1486, 1863, 3827, 4119, 6040, 6747
HindII GTYRAC 6 cut(s) 1486, 1863, 3827, 4119, 6040, 6747
HindIII AAGCTT 4 cut(s) 2805, 3600, 5859, 6131
HpaI GTTAAC 1 cut(s) 1486
HpaII CCGG 4 cut(s) 1349, 3704, 6549, 6886
Hpy99I CGWCG 2 cut(s) 4682, 5684
HpyCH4IV ACGT 3 cut(s) 3733, 4007, 4677
HpySE526I ACGT 3 cut(s) 3733, 4007, 4677
Hsp92I GRCGYC 2 cut(s) 4223, 4677
HspAI GCGC 5 cut(s) 313, 2231, 2855, 3549, 5698
KflI GGGWCCC 1 cut(s) 4034
Kpn2I TCCGGA 1 cut(s) 6548
KpnI GGTACC 1 cut(s) 3255
Ksp22I TGATCA 6 cut(s) 478, 1204, 1444, 2404, 5293, 6831
KspAI GTTAAC 1 cut(s) 1486
KspI CCGCGG 1 cut(s) 4722
LguI GCTCTTC 1 cut(s) 135
LmnI GCTCC 7 cut(s) 460, 1592, 2173, 3496, 3769, 4519, 6610
MaeII ACGT 3 cut(s) 3733, 4007, 4677
MfeI CAATTG 4 cut(s) 1964, 2925, 4148, 5297
MhlI GDGCHC 6 cut(s) 1376, 2989, 3766, 3845, 4201, 4851
MlyI GAGTC 8 cut(s) 712, 1022, 1868, 4114, 4370, 4540, 5065, 6035
MmeI TCCRAC 5 cut(s) 1050, 1642, 2930, 5149, 5713
Mph1103I ATGCAT 2 cut(s) 1603, 5752
MroI TCCGGA 1 cut(s) 6548
MslI CAYNNNNRTG 5 cut(s) 693, 1833, 2792, 3420, 3633
MspA1I CMGCKG 9 cut(s) 1742, 2111, 3208, 4610, 4709, 4721, 4745, 4751, 5236
MspI CCGG 4 cut(s) 1349, 3704, 6549, 6886
MunI CAATTG 4 cut(s) 1964, 2925, 4148, 5297
MvnI CGCG 3 cut(s) 2656, 3549, 4721
NcoI CCATGG 1 cut(s) 36
NdeI CATATG 3 cut(s) 124, 1344, 1762
NmeAIII GCCGAG 1 cut(s) 6562
NsiI ATGCAT 2 cut(s) 1603, 5752
NspI RCATGY 7 cut(s) 692, 1106, 1838, 3419, 3649, 4169, 5140
NspV TTCGAA 3 cut(s) 1979, 2135, 6154
PaqCI CACCTGC 1 cut(s) 6691
PasI CCCWGGG 1 cut(s) 1108
PceI AGGCCT 2 cut(s) 3407, 4022
PciI ACATGT 1 cut(s) 1834
PciSI GCTCTTC 1 cut(s) 135
PcsI WCGNNNNNNNCGW 1 cut(s) 1899
PflMI CCANNNNNTGG 1 cut(s) 1628
PfoI TCCNGGA 1 cut(s) 3035
PleI GAGTC 8 cut(s) 711, 1021, 1867, 4114, 4369, 4540, 5064, 6035
PpsI GAGTC 8 cut(s) 711, 1021, 1867, 4114, 4369, 4540, 5064, 6035
Ppu21I YACGTR 1 cut(s) 4008
PpuMI RGGWCCY 1 cut(s) 4034
PscI ACATGT 1 cut(s) 1834
PshAI GACNNNNGTC 1 cut(s) 1637
PshBI ATTAAT 2 cut(s) 840, 2778
Psp5II RGGWCCY 1 cut(s) 4034
PspEI GGTNACC 4 cut(s) 1390, 1516, 1925, 4490
PspFI CCCAGC 2 cut(s) 2771, 3830
PspOMI GGGCCC 1 cut(s) 4197
PspPPI RGGWCCY 1 cut(s) 4034
PstNI CAGNNNCTG 4 cut(s) 350, 2413, 4745, 5006
PvuII CAGCTG 7 cut(s) 1742, 3208, 4610, 4709, 4745, 4751, 5236
RseI CAYNNNNRTG 5 cut(s) 693, 1833, 2792, 3420, 3633
SacII CCGCGG 1 cut(s) 4722
SalI GTCGAC 2 cut(s) 3825, 6745
SapI GCTCTTC 1 cut(s) 135
ScaI AGTACT 2 cut(s) 97, 4210
SchI GAGTC 8 cut(s) 712, 1022, 1868, 4114, 4370, 4540, 5065, 6035
SduI GDGCHC 6 cut(s) 1376, 2989, 3766, 3845, 4201, 4851
Sfr303I CCGCGG 1 cut(s) 4722
SfuI TTCGAA 3 cut(s) 1979, 2135, 6154
SgrBI CCGCGG 1 cut(s) 4722
SinI GGWCC 7 cut(s) 1916, 2882, 2903, 4034, 4178, 5075, 5357
SmiMI CAYNNNNRTG 5 cut(s) 693, 1833, 2792, 3420, 3633
SmlI CTYRAG 7 cut(s) 22, 1853, 4068, 5100, 5835, 5942, 6318
SmoI CTYRAG 7 cut(s) 22, 1853, 4068, 5100, 5835, 5942, 6318
SpeI ACTAGT 2 cut(s) 4205, 6281
SseBI AGGCCT 2 cut(s) 3407, 4022
SspI AATATT 2 cut(s) 838, 3507
StuI AGGCCT 2 cut(s) 3407, 4022
StyI CCWWGG 7 cut(s) 36, 1238, 1590, 3246, 4317, 4806, 6062
TaiI ACGT 3 cut(s) 3736, 4010, 4680
TatI WGTACW 3 cut(s) 95, 4100, 4208
TauI GCSGC 5 cut(s) 2114, 4721, 4724, 5370, 6440
TspGWI ACGGA 3 cut(s) 1845, 3277, 5697
Van91I CCANNNNNTGG 1 cut(s) 1628
VpaK11BI GGWCC 7 cut(s) 1916, 2882, 2903, 4034, 4178, 5075, 5357
VspI ATTAAT 2 cut(s) 840, 2778
XbaI TCTAGA 4 cut(s) 1309, 1549, 1808, 6896
XceI RCATGY 7 cut(s) 692, 1106, 1838, 3419, 3649, 4169, 5140
XcmI CCANNNNNNNNNTGG 4 cut(s) 1625, 2122, 3365, 5085
XmaJI CCTAGG 1 cut(s) 1238
XmiI GTMKAC 4 cut(s) 3826, 4397, 6057, 6746
ZraI GACGTC 1 cut(s) 4678
ZrmI AGTACT 2 cut(s) 97, 4210
Zsp2I ATGCAT 2 cut(s) 1603, 5752
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.