Rroxscaffold_3G00253720

Agenet domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0002656
Physical Location & Seq
Forward (+)
47782067 .. 47794003
11937 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00253720.1

Sequence Viewer

Length: 7059 bp
ATGGAGTATGATGACAATGATTTTCAAAGCCAGAATCTTCAATTAGCTGGTGAAGGGAGCACCAATTGTCCTCCAGTTTTACAGCCATATGGTCTTCCCAAATTTGATTTTGATGACAGCCTTCAGGGGCATTTGAGGTTTGATAGTTTGGTTGAAACTGAGGTTTTTCTTGGCATTGAGAGTAGCGAAGCTAACAACTGGATTGAGGATTTCTCTCGTGGGAGTAGTGGGATAGAGTTTAGTTCAAGTGCAGTAGAATCGTGCTCTATATCAAGGCGGAACAATGTTTGGTCTGAGGCCACGTCCTCAGAATCTGTTGAGATGCTGTTAAAATCTGTTGGGCAGGAAGAAATAATTCCCGCTCAGACTATCTTGGAGGAATCAGATGCCTGTAAGGAAGTGGGTTGCATAAAACAGATGGAGCCTAATTTGAAGAATGATGATAACATTCTCACTGAAACAGGGGATGTTAGAGATCTACATTCTACTTTACTGCTAGATGAAACTGCCGGAAACCTTTCTGGCTTAGATAATGTTGGAGATCAGCCTCGGCTTGAGGATGCTTCACAAACTCAGGAAGTTGAATTACCGGTTGATGGAAATTCAAGTGTTTTGGATCCAAATTCTGTCAGTGATCGGGATAGGCTGCCTGTAACTAAAGGGAGCCTGACTGATGATAAAGGCAAGGATGTGAATAATATGGAATTTGATAATTTAGTTGATTATCCTCCAGATAAAAAAATGCATGAAAATTCATCTGCTTCAGAGATGCAAGTTGATAATATGATGACTTCTGTGCAAAGTATTATTACAAGCAGTGATGAGTTGAATAATCAAGATGTTCCACATCATATAGTGGATATTAGTGAAGAGAATCCAGGCAGTCATGATGTGGCAAGCATAGATAGTCAATGTAGGAATGATTTGGACAAACCTCATTGTTTAGCGTCAGAGGTGGAGTCTGGAAAAAAAGAAATTGCAAATGAAGATACTGTTATTGATGTGCAGGAAGCGTCTAAAATTATGCTGCAAGGGGATTCTATTTTACATACAATGGGAGGATGCAGTGATAAGGAATGTCCTGGAGTTCTTACTGAGACCAATAAATGTGAAGATATGGTAGCGTCTAAGGACACTGATGTTGGTGGCGATCAATCCACAATGACCTACACAAATGATGCTGGGTATGGAATTGAGGTTAGGAACAACCTTGGCGAAATTTCTTCCAGTCTAGATCCTATTCTGAAAGGGGAATCTGATTTACAGACAGTGGACAGATGCACTGACAGGGAATGCCCTGAAGTTCTTGATGACACCAACAAATGTGAAGATGTTGTTATCTCTTTTAAAGACACAAATACTGGTGGAGTTCCAGCTAAGTTGGACAAAAATGACAGTGGTTATGGCACTGAGGATTGGAACAGCAATACTGGAGTTTTGTCTGGTCTAGATCCTACCCTCAAAGGGGATTCTAACTTGCATACAATGGATGTATGTACTGTCAAGGAATCCCTTGAAGTTCCTTCTGTCTCGTTGAAAGCTACAGACACTGGTGGTGATCAATCTAAATTACACGCACCTGACTTATCACCAATGGCCTATGGGAATGACACTGGGCATGCAGTTGAACTTGGCAACAGTGTTTCTGCCTGTGTAAGCCCTACCTTGAGAACGGATAATGATTTACATACAGTGGATGCAAGTAGTGACAGGAAATGCCCTGATGTTCCTGTGGAGACCACTAAATGTGAAGACATAGTGTTGTCCAAAGGCACAGATACTGCCGGTGATCAATCTAACATAAGCCCACATGTTTTATCACCTGTAGCCTACAAAAGTGATACTGTGTATGCAGATGAGGTTGGGAGCAGCAATTCTGGATTTTCGTCCGGTCTAGACTCTAGATTGAAGGTGAATTCTGGAAAGAGCACCGAGGAGGATGCATTAAAAAGTGGTGGCCAACCCCTTGATAGTGAGATTTTGGTCAGAAAGTCTGAGGCTTCTATGGCAGTCATTGAGGAAACTGAGGTTTTCAAAGGCAGAGGTGATGAGAATCCGGAAGTTCATTCTAATTTGACTCCAACACGCTCTTCAGCCGAAATGCCTAGTGAAGTGCATGTGACTGGAGCTTCTAAAAGTCTTCATGATGCTTTTGGAGTTTCTGCCGAGGAATTAAATGAAGAAAGCCATGTGTCACCTTCTAATCTGAAGGAGTCTACAGAAAGGTGTTCTGAAAATGAAGTTTGCAAAGAAGGTATATCTGGTAAATGTGACCAGGATCTCTCTTACAGTGAAAAGGATACTACAAATTTGCCTGTTGAATCTAATAGTATGGATTTGGAGTTTGTTGGATCTACTGGTAAGGGAGATGAGTCCTCATCTTTGTGTGAAGATTCTGCAGAAAAAGAGTTGATTGTTCCAACACTAAAACATGATGTTGATGGCAATGCATCAGTGGCTGATGTTCCCATGGAAAGTTGTGATCTGGCTTCTTGCGGCACAAGGGATGTTGTTCCCTTGTCTTCTGAGAATGGTGCAACCACACATATTCATAACGTATCAGTGGTTCCTGCAGCACCTTCTCCTGCTATGGGATCACACTCGGACAATAAAGGAGAAACTGCAATCGAAATATCCAAAGATGCAAGTGTGTCAGGTACAGTGTCATCACCTATGGTAGAATCTGGACCAGGCCCTGTTTGTGAAGCTGAAAAAGGTGCTTCTTGTGATACTGCTGGACAACTGTTATGCAATACACTTGATCAGTCTCTGCCAATTTCAGTTAGTTGCAATACTGAAAACCAAAGTGAACCTGGAACAGCAGTTGCTATTGAAATTAGCAAGAGAAGCACCATGGATATGGAAGTAAAACGAGGTGATGATGCTGAAGCTCAAGATGGAGCAACAATAGAAAACAAATCTACCACAGAAGACAAATCTGAAAAGGCATCAGCGAATGTAACAGGTGATGCTTTTGGAAGTTTTGAAGGTCCAATGCTTTCTACTTGTTTGCCCGAATCTTGTTCTAAGTCTGCTGATCCCTACCAGCCTGGTTGTGATTCTCCCAAAGTTTTTGGAACAACTGAGATTCCTGAGACTAAACATGAAAAAGGCAATATCAAGGAGTCGACAAATCAGAATGCCGCAGATACTAATGTAGTTGGGGATGGCGGCAAACATTCTTCTAATTCCTCGAATCCAACTGGAAATGATGTCCCCAAAGATCAGGGATATGGCACCTCAGATATCACTTCCTTTGCAGATTTGCCTATAGGGGTTGCTGCCAACAGCTCGCAGCCCTTTCCTGCTATTTCAGCTCCCAAAATTGTGGATGGGTCTCAAACAAATTCTGCCTCAGGCCAGATGGATGCAAATATCTCGCAAGAAATTTCTCGTGGAGGTGATGGAGAGATTGCTAGTGGTGGTTCTAAAGGTACCACAGAACGGAAAAGAAGGCGGGCATCTAGTAAGGGAACGGGTAAAGAGAGTGCTAAGAAGGGAACTGTGAAAGCGACAACTCCTATGAGACAAGCAGACAGAGGAGACAAATCTAGCAGTGTGCCCCTGGGGAAATCTGGAATTTTCCAGTTTGCGCATCCAAGTGACATACAGTACTTTGGGCTTGTGGATGGTAGCTCAAAAACTTATTCAGTTCTTACTAGTGCGACATCCAGTCTGCCAGATCTGAATTCTTCTGCTCCAGCATCTCTAGTATTTCAGCAGCCTTTCACCGATCTGCAGCAAGTGCAATTACGTGCTCAGATCTTTGTTTATGGAGCTTTGATTCAAGGAACAGCACCTGATGAAGCTTATATGGTGTCAGCATATGGAGGACCTGATGGTGGAAGGAGTATCTGGGAGAATGCGTGGCGTAAGTGCATAGAGAGGCTACACAGTCAAAAATCCACCCCTATTAATCCAGAAACCCCTTTGCAGTCATCCTCTGACCTGAGATTTACAGGGGGTAGGGTTCCTGATCAAGCTGGTAAACAAAGTGCATTCCAGGGTAAAGTTATCTCCTCACCTGCTGGTCGAGCCAGCACAAAGGGTATTCCACCTACTGCGAGTCCCATGATACCAATTTCATCACCACTTTGGAGCATTCCTACCCCTGCTTGTGAGGCCCCTCAATATAGTGTTCTGCCGAGAGGTTCGGTAATGGATTATCAGCAGGCCCATACTCCATTGCATCCTTTCCAAACATCACCCATAAGGAACATTGTTGGACAAAATTCCTCGTGGATGTCTCAGTCCCCGTTTCGGGGTCCCTGGGTTCCTTCCCCACAGTCATCTGCAGCTGAAACCAGTATTCGTTTCTCAGCATTTCCTAGTACAGAGTCAGTGCTGCTGACTCCTGTAAAAGAAACAACTTCATCACAAGTATCCAGTATAAAGCATGCCTCCTCGGTTGTTACTGGTCAAACTGGGGTTACAACCAGTGTTTTTGCAGGGGTTTCTCCACTGCTTGACTCAAAGAAAGTGGGAGTGGCATCACCTGGCGAGCCTTCATCTCAACCTAAGTCTAGAAAAAGAAAAAAGATCTCAGATTCTAAGGAACTTGGTCCGATTAGTTTGCAGCCTCAATCTGAACCTGAGTCAGCTATAACCCTGGCTGTTACTAGTAGTGTATCTACATCTGTTGTAGTCACTGCCCCAAGTACCTTTGTGTCTAAGGCCATGCCAGAAAAATTGGCTATGTCGGTATCCTCCGATCACCTCAAGAAAGCAGATCTTGGTTTGGAGCAGAGGGCAACTTTATCAAAAGAGACTCTTAGTAAAGCTAAGGAGGCTAGGCAGCAAGCAGAGGAAGCTTCTGCTCATGCTGCTGCAGCTGTTGGTCACAGTCAAGAAATATGGAATCAGTTGGATAAGCAGAAACATTCCAGATTGACATCAGATGCCGAAGCCAAATTAGCTTCTGCTGCTGTTGCTGTAGCTGCCGCCGCTGCTGTTGCAAAAGCAGCAGCTGCAGCTGCCAATGTTGCGGCAAATGCTGCATTGCAAGCAGTACTGATGGCTGAGGAAGCCTATGGGAATCAAAGCGAAAGTGTGATAGATTTGAGTACTGATGGAGTGAATGCATTGGGGTTGGCTACTCCAGGATCTGTTTTTAGGGCTGAGGATGGAACAAACAGTTCAAGTTCAATCCTTTTTGCGGCTAGGGAGGCTGCTAGAAGAAGGGTTGAAGCTGCATCTGCAGCCTCAAAGCGAGCTGAAAATATGGACGCCATAGTAAAAGCTGCTGAGCTAGCCGCAGAAGCTGTATCACAAGCTGGAACAGTTGTTGCTATGGGTGATCCTGTGCCTTTGAGCGATTTGGTAAAAGCTGGTCCTGAGGGTTATTGGAAAGCACCCCAAGTATCTTCAGAGATTGTTAAGAAATCAAATGACGGAATGAGAGAACAATCAAATTTTTCCACTGTTGGAGAAGATTCAGATAAGGAAGAAACACAGATCACTGCAACTAAGAAATCGCCTACAGTCAGTGAAAGGGTTACTGAGATTATAAAGTCTGCTTTGCCCGCTGCTGGGAATGATCTTGGAAAAACTGCAGAAGCGTCCAAAGATAGCTGCATCGTGGAGGGTTCTCAAGTAGAGGTTTTCAAAGAAGGGGGTGGATTTGCAGTCGGCTGGTTCACAGCTACTGTATTGAGTTTGCAGGATGGCAAAGCTTGTGTATGTTACACTGAGCTTCAGTCAGACGAAGGCTCAGGGAAACTACAGGAATGGGTGGCACTTGACAGCAAAGACGATAAGCCACCAAAAATACGCGTTGCTCGCCTTCTTACCCCCTCCCTTGAAGGAACAAGGAAAAGGCGGAGAGAAGCAATGGCAGATTACGCTTGGTCTGTTGGAGACAAAGTTGATGCCTGGATACAAAACAGCTGGTGGGAAGGAGTTGTGACTGAAAAAAACAAGAAAGATGAAACTATACTAAAAGTCCACTTTCCAGCTCAAGGGGAGACATCACATGTTAAAGCTTGGCATCTTCGGCCTTCTCTGATATGGAAGGATGGGGAATGGGTTGAATGGTCCAGTGTGCGGAATGACGGCTCTTCCATGGAGGATGGCTTGCCGCAGGAAAAGCGAATCAAATTGGGCAGTTCTACAGTGGAAGGAAAAGGGAAGGATATGACTTTAAAAAGCAATGTTCTTGATTCAGGAAAACCTGAAGAGCCAAGGTTGCTGAATTTGTCTGCAAATGAAAAGGTATTTAATATAGGTAAAAATACCAGGATCGAGAATAAGCTTGATGGAGCCAGAACAAATCGGACAGGGTTGCAGAAGGAAGGATCAGTAAAATTTGGTATTCCGAAGCCCAAAAGAAAATTTATGGAAGTTAGCAAGCATTATGTGGCCAATCAGACTAGTAAGGTTAACGAGTCTAATGATTCACTGAAATTTGCGAAATATTTGATGCCACAATCATCAGGTTTCCGTGCATTGAAAAATACATCTAAATTTGATACAAAGGACAAAGAGGGGGCTGATAACAAGCTAAGGGGTTTTAGATCTGAAAAGCAGCGAAGTATGTCGGATAAAACAGTTCCTCAGAAGGACAACTTTTCAATAGATTTAGTTTCTGCTGCGGATGGCTCCAGTAAGATGGACCATACAAAAAAGGTCAAAGACTCTGTAAGACAAGCTGAGGGTTTATCTGGCAAGCGCAACATATTTGAGACGGGATCAGGTTATAGCTCAGATGGAAGAGCACAGGGCACAGCCATGTTTTCTTCACGAACTCCGTCGTCAGATTTACCTCCATCCAAGAAGGTAGCCACTACAACTGCTAAATCTGAACGGGGAAATAAAGGAAATTTTGCACCAGTTGTTGGAAAGTTGGGTAAAATTGAGGAGGGCAAAGGTACAAGTAGTAATCCTGTTAAGTCAACTTCAGAAGTAGTCGAGCCTCGTAGATCAAATCGCAGAATTCAGCCAACATCAAGACTATTAGAAGGACTGCAAAGTTCGTTGAGCATTTCAAAGATTCCTTCCGTTTCACATGAGAAGCCCAGAAGTCAGAATAGAAATGCTTCTAGAGGGAGTAACCATGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Agenet

Protein Analysis

2352

Amino Acids

250.33

Weight (kDa)

4.96

Isoelectric Point (pI)

49.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Agenet PF05641 1862 - 1914 1.4e-10 Agenet domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 5507
AarI CACCTGC 1 cut(s) 4033
Acc16I TGCGCA 1 cut(s) 3594
Acc36I ACCTGC 1 cut(s) 4033
Acc65I GGTACC 1 cut(s) 3434
AccB1I GGYRCC 2 cut(s) 3236, 3434
AccB7I CCANNNNNTGG 1 cut(s) 6833
AccBSI CCGCTC 1 cut(s) 362
AccI GTMKAC 2 cut(s) 2216, 3128
AccII CGCG 1 cut(s) 5772
AccIII TCCGGA 1 cut(s) 2056
AcoI YGGCCR 2 cut(s) 1957, 6357
AcyI GRCGYC 1 cut(s) 5226
AfaI GTAC 9 cut(s) 1498, 2659, 3436, 3614, 4333, 4660, 5010, 5065, 6868
AflIII ACRYGT 3 cut(s) 1810, 5770, 5971
AgeI ACCGGT 1 cut(s) 589
AhdI GACNNNNNGTC 1 cut(s) 3672
AhlI ACTAGT 3 cut(s) 3659, 4619, 6368
AjiI CACGTC 1 cut(s) 303
AjuI GAANNNNNNNTTGG 2 cut(s) 2074, 2106
Alw21I GWGCWC 5 cut(s) 62, 266, 1931, 3760, 6715
Aor13HI TCCGGA 1 cut(s) 2056
AseI ATTAAT 1 cut(s) 3915
AsiGI ACCGGT 1 cut(s) 589
Asp700I GAANNNNTTC 3 cut(s) 354, 517, 7033
Asp718I GGTACC 1 cut(s) 3434
AspLEI GCGC 2 cut(s) 3595, 6669
AsuNHI GCTAGC 1 cut(s) 5248
AvaII GGWCC 8 cut(s) 2687, 2990, 3833, 4265, 4562, 5330, 6033, 6610
AxyI CCTNAGG 2 cut(s) 3355, 5334
BaeGI GKGCMC 2 cut(s) 3564, 6722
BaeI ACNNNNGTAYC 2 cut(s) 3141, 3174
BalI TGGCCA 2 cut(s) 1959, 6359
BamHI GGATCC 1 cut(s) 616
BanI GGYRCC 2 cut(s) 3236, 3434
BarI GAAGNNNNNNTAC 2 cut(s) 570, 602
BauI CACGAG 3 cut(s) 216, 3393, 4237
BbsI GAAGAC 5 cut(s) 86, 1758, 2132, 2514, 2937
Bbv12I GWGCWC 5 cut(s) 62, 266, 1931, 3760, 6715
BbvCI CCTCAGC 3 cut(s) 5019, 5118, 6648
BceAI ACGGC 1 cut(s) 6067
BcgI CGANNNNNNTGC 8 cut(s) 240, 274, 1922, 1956, 3360, 3394, 4555, 4589
BciVI GTATCC 4 cut(s) 2293, 4393, 4714, 5868
BclI TGATCA 4 cut(s) 1558, 1789, 2761, 3976
BcuI ACTAGT 3 cut(s) 3659, 4619, 6368
BfuAI ACCTGC 1 cut(s) 4033
BfuI GTATCC 4 cut(s) 2293, 4393, 4714, 5868
BglII AGATCT 6 cut(s) 475, 3682, 3762, 4539, 4729, 6512
BlpI GCTNAGC 1 cut(s) 5244
BmcAI AGTACT 3 cut(s) 3614, 5010, 5065
Bme18I GGWCC 8 cut(s) 2687, 2990, 3833, 4265, 4562, 5330, 6033, 6610
BmeRI GACNNNNNGTC 1 cut(s) 3672
BmgBI CACGTC 1 cut(s) 303
BmrI ACTGGG 2 cut(s) 1623, 4434
BmtI GCTAGC 1 cut(s) 5252
BmuI ACTGGG 2 cut(s) 1623, 4434
BpiI GAAGAC 5 cut(s) 86, 1758, 2132, 2514, 2937
BplI GAGNNNNNCTC 2 cut(s) 197, 229
BpmI CTGGAG 8 cut(s) 57, 714, 1104, 1452, 2145, 3684, 5082, 6583
Bpu10I CCTNAGC 6 cut(s) 4782, 5019, 5118, 5710, 6500, 6648
Bpu1102I GCTNAGC 1 cut(s) 5244
BpuEI CTTGAG 6 cut(s) 575, 1687, 2877, 4703, 5574, 5940
BsaAI YACGTR 1 cut(s) 3755
BsaBI GATNNNNATC 2 cut(s) 2052, 4891
BsaHI GRCGYC 1 cut(s) 5226
BsaI GGTCTC 3 cut(s) 1091, 1730, 3342
BsaWI WCCGGW 3 cut(s) 589, 1889, 2056
BsaXI ACNNNNNCTCC 4 cut(s) 3851, 3881, 5847, 5877
Bse118I RCCGGY 2 cut(s) 589, 1784
Bse21I CCTNAGG 2 cut(s) 3355, 5334
Bse3DI GCAATG 5 cut(s) 2452, 4184, 4997, 5835, 6154
Bse8I GATNNNNATC 2 cut(s) 2052, 4891
BseAI TCCGGA 1 cut(s) 2056
BseJI GATNNNNATC 2 cut(s) 2052, 4891
BseMI GCAATG 5 cut(s) 2452, 4184, 4997, 5835, 6154
BseRI GAGGAG 5 cut(s) 1949, 3555, 4009, 4393, 6869
BseSI GKGCMC 2 cut(s) 3564, 6722
BseYI CCCAGC 2 cut(s) 1181, 5528
BsgI GTGCAG 2 cut(s) 270, 1025
Bsh1236I CGCG 1 cut(s) 5772
BshNI GGYRCC 2 cut(s) 3236, 3434
BshTI ACCGGT 1 cut(s) 589
BsiHKAI GWGCWC 5 cut(s) 62, 266, 1931, 3760, 6715
BsiSI CCGG 5 cut(s) 510, 590, 1785, 1890, 2057
BslFI GGGAC 4 cut(s) 3200, 4053, 4237, 4251
BsmBI CGTCTC 1 cut(s) 6674
BsmFI GGGAC 4 cut(s) 3200, 4053, 4237, 4251
BsmI GAATGC 6 cut(s) 1298, 3145, 3868, 3998, 4101, 5083
Bso31I GGTCTC 3 cut(s) 1091, 1730, 3342
Bsp1286I GDGCHC 7 cut(s) 62, 266, 1931, 3564, 3760, 6715, 6722
Bsp13I TCCGGA 1 cut(s) 2056
Bsp1720I GCTNAGC 1 cut(s) 5244
Bsp19I CCATGG 3 cut(s) 2469, 2853, 6060
BspEI TCCGGA 1 cut(s) 2056
BspFNI CGCG 1 cut(s) 5772
BspHI TCATGA 2 cut(s) 886, 2143
BspMAI CTGCAG 8 cut(s) 2401, 2575, 3741, 4297, 4831, 4972, 5200, 5554
BspMI ACCTGC 1 cut(s) 4033
BspOI GCTAGC 1 cut(s) 5252
BspQI GCTCTTC 4 cut(s) 2095, 6061, 6168, 6703
BspT107I GGYRCC 2 cut(s) 3236, 3434
BspTNI GGTCTC 3 cut(s) 1091, 1730, 3342
BsrBI CCGCTC 1 cut(s) 362
BsrDI GCAATG 5 cut(s) 2452, 4184, 4997, 5835, 6154
BsrFI RCCGGY 2 cut(s) 589, 1784
BssAI RCCGGY 2 cut(s) 589, 1784
BssNI GRCGYC 1 cut(s) 5226
BssSI CACGAG 3 cut(s) 216, 3393, 4237
BssT1I CCWWGG 5 cut(s) 1210, 2469, 2853, 6060, 6179
Bst2BI CACGAG 3 cut(s) 216, 3393, 4237
Bst6I CTCTTC 5 cut(s) 864, 2095, 6061, 6168, 6703
BstACI GRCGYC 1 cut(s) 5226
BstAPI GCANNNNNTGC 3 cut(s) 3745, 4967, 4994
BstBAI YACGTR 1 cut(s) 3755
BstDSI CCRYGG 3 cut(s) 2469, 2853, 6060
BstFNI CGCG 1 cut(s) 5772
BstHHI GCGC 2 cut(s) 3595, 6669
BstNSI RCATGY 5 cut(s) 1622, 1814, 2120, 4400, 5975
BstSLI GKGCMC 2 cut(s) 3564, 6722
BstUI CGCG 1 cut(s) 5772
BstV2I GAAGAC 5 cut(s) 86, 1758, 2132, 2514, 2937
BstXI CCANNNNNNTGG 3 cut(s) 2860, 3328, 6607
Bsu36I CCTNAGG 2 cut(s) 3355, 5334
BsuI GTATCC 4 cut(s) 2293, 4393, 4714, 5868
BtgI CCRYGG 3 cut(s) 2469, 2853, 6060
BtrI CACGTC 1 cut(s) 303
BtsI GCAGTG 6 cut(s) 823, 1072, 3562, 4460, 4647, 5457
BveI ACCTGC 1 cut(s) 4033
CciI TCATGA 2 cut(s) 886, 2143
CfoI GCGC 2 cut(s) 3595, 6669
Cfr10I RCCGGY 2 cut(s) 589, 1784
CseI GACGC 5 cut(s) 936, 1002, 1113, 5234, 5547
Csp6I GTAC 9 cut(s) 1497, 2658, 3435, 3613, 4332, 4659, 5009, 5064, 6867
CspAI ACCGGT 1 cut(s) 589
CspCI CAANNNNNGTGG 2 cut(s) 4461, 4496
CviQI GTAC 9 cut(s) 1497, 2658, 3435, 3613, 4332, 4659, 5009, 5064, 6867
DraI TTTAAA 2 cut(s) 1348, 6141
DriI GACNNNNNGTC 1 cut(s) 3672
EaeI YGGCCR 2 cut(s) 1957, 6357
Eam1104I CTCTTC 5 cut(s) 864, 2095, 6061, 6168, 6703
Eam1105I GACNNNNNGTC 1 cut(s) 3672
EarI CTCTTC 5 cut(s) 864, 2095, 6061, 6168, 6703
EciI GGCGGA 2 cut(s) 292, 5833
Eco130I CCWWGG 5 cut(s) 1210, 2469, 2853, 6060, 6179
Eco31I GGTCTC 3 cut(s) 1091, 1730, 3342
Eco32I GATATC 1 cut(s) 3247
Eco47I GGWCC 8 cut(s) 2687, 2990, 3833, 4265, 4562, 5330, 6033, 6610
Eco81I CCTNAGG 2 cut(s) 3355, 5334
EcoO109I RGGNCCY 4 cut(s) 2693, 3833, 4123, 4265
EcoRI GAATTC 3 cut(s) 1915, 3688, 6930
EcoRV GATATC 1 cut(s) 3247
EcoT14I CCWWGG 5 cut(s) 1210, 2469, 2853, 6060, 6179
EcoT22I ATGCAT 4 cut(s) 747, 1945, 2452, 5083
ErhI CCWWGG 5 cut(s) 1210, 2469, 2853, 6060, 6179
Esp3I CGTCTC 1 cut(s) 6674
FalI AAGNNNNNCTT 6 cut(s) 4716, 4748, 4755, 4787, 6548, 6580
FaqI GGGAC 4 cut(s) 3200, 4053, 4237, 4251
FauI CCCGC 3 cut(s) 367, 3450, 5530
FauNDI CATATG 2 cut(s) 88, 3826
FbaI TGATCA 4 cut(s) 1558, 1789, 2761, 3976
FblI GTMKAC 2 cut(s) 2216, 3128
FspI TGCGCA 1 cut(s) 3594
GlaI GCGC 2 cut(s) 3594, 6668
GsaI CCCAGC 2 cut(s) 1185, 5532
GsuI CTGGAG 8 cut(s) 57, 714, 1104, 1452, 2145, 3684, 5082, 6583
HapII CCGG 5 cut(s) 510, 590, 1785, 1890, 2057
HgaI GACGC 5 cut(s) 936, 1002, 1113, 5234, 5547
HhaI GCGC 2 cut(s) 3595, 6669
Hin1I GRCGYC 1 cut(s) 5226
Hin6I GCGC 2 cut(s) 3593, 6667
HinP1I GCGC 2 cut(s) 3593, 6667
HincII GTYRAC 3 cut(s) 3129, 6379, 6891
HindII GTYRAC 3 cut(s) 3129, 6379, 6891
HindIII AAGCTT 5 cut(s) 3807, 4809, 5670, 5979, 6248
HpaI GTTAAC 1 cut(s) 6379
HpaII CCGG 5 cut(s) 510, 590, 1785, 1890, 2057
Hpy166II GTNNAC 9 cut(s) 1273, 2217, 2810, 3129, 3989, 5637, 5944, 6379, 6891
Hpy8I GTNNAC 9 cut(s) 1273, 2217, 2810, 3129, 3989, 5637, 5944, 6379, 6891
Hpy99I CGWCG 1 cut(s) 6751
HpyCH4IV ACGT 3 cut(s) 302, 2556, 3754
HpySE526I ACGT 3 cut(s) 302, 2556, 3754
Hsp92I GRCGYC 1 cut(s) 5226
HspAI GCGC 2 cut(s) 3593, 6667
KflI GGGWCCC 1 cut(s) 4265
Kpn2I TCCGGA 1 cut(s) 2056
KpnI GGTACC 1 cut(s) 3438
Ksp22I TGATCA 4 cut(s) 1558, 1789, 2761, 3976
KspAI GTTAAC 1 cut(s) 6379
LguI GCTCTTC 4 cut(s) 2095, 6061, 6168, 6703
MaeII ACGT 3 cut(s) 302, 2556, 3754
MbiI CCGCTC 1 cut(s) 362
MfeI CAATTG 1 cut(s) 64
MhlI GDGCHC 7 cut(s) 62, 266, 1931, 3564, 3760, 6715, 6722
MlsI TGGCCA 2 cut(s) 1959, 6359
MluI ACGCGT 1 cut(s) 5770
MluNI TGGCCA 2 cut(s) 1959, 6359
Mox20I TGGCCA 2 cut(s) 1959, 6359
Mph1103I ATGCAT 4 cut(s) 747, 1945, 2452, 5083
MroI TCCGGA 1 cut(s) 2056
MroXI GAANNNNTTC 3 cut(s) 354, 517, 7033
MscI TGGCCA 2 cut(s) 1959, 6359
MslI CAYNNNNRTG 4 cut(s) 2383, 2858, 3600, 6725
Msp20I TGGCCA 2 cut(s) 1959, 6359
MspA1I CMGCKG 7 cut(s) 4298, 4832, 4946, 4967, 4973, 5525, 5886
MspI CCGG 5 cut(s) 510, 590, 1785, 1890, 2057
MunI CAATTG 1 cut(s) 64
Mva1269I GAATGC 6 cut(s) 1298, 3145, 3868, 3998, 4101, 5083
MvnI CGCG 1 cut(s) 5772
NcoI CCATGG 3 cut(s) 2469, 2853, 6060
NdeI CATATG 2 cut(s) 88, 3826
NheI GCTAGC 1 cut(s) 5248
NmeAIII GCCGAG 3 cut(s) 529, 2191, 4170
NmuCI GTSAC 8 cut(s) 1706, 2119, 2193, 2270, 3602, 4645, 4838, 5902
NsbI TGCGCA 1 cut(s) 3594
NsiI ATGCAT 4 cut(s) 747, 1945, 2452, 5083
NspI RCATGY 5 cut(s) 1622, 1814, 2120, 4400, 5975
PaeI GCATGC 2 cut(s) 1622, 4400
PagI TCATGA 2 cut(s) 886, 2143
PaqCI CACCTGC 1 cut(s) 4033
PasI CCCWGGG 2 cut(s) 3565, 4269
PciI ACATGT 2 cut(s) 1810, 5971
PciSI GCTCTTC 4 cut(s) 2095, 6061, 6168, 6703
PctI GAATGC 6 cut(s) 1298, 3145, 3868, 3998, 4101, 5083
PdmI GAANNNNTTC 3 cut(s) 354, 517, 7033
PflMI CCANNNNNTGG 1 cut(s) 6833
PfoI TCCNGGA 2 cut(s) 1081, 5098
PinAI ACCGGT 1 cut(s) 589
Ppu21I YACGTR 1 cut(s) 3755
PpuMI RGGWCCY 2 cut(s) 3833, 4265
PscI ACATGT 2 cut(s) 1810, 5971
PshBI ATTAAT 1 cut(s) 3915
PsiI TTATAA 1 cut(s) 5507
Psp5II RGGWCCY 2 cut(s) 3833, 4265
PspFI CCCAGC 2 cut(s) 1181, 5528
PspPPI RGGWCCY 2 cut(s) 3833, 4265
PsrI GAACNNNNNNTAC 2 cut(s) 2550, 2582
PstI CTGCAG 8 cut(s) 2401, 2575, 3741, 4297, 4831, 4972, 5200, 5554
PvuII CAGCTG 5 cut(s) 4298, 4832, 4967, 4973, 5886
RsaI GTAC 9 cut(s) 1498, 2659, 3436, 3614, 4333, 4660, 5010, 5065, 6868
RsaNI GTAC 9 cut(s) 1497, 2658, 3435, 3613, 4332, 4659, 5009, 5064, 6867
RseI CAYNNNNRTG 4 cut(s) 2383, 2858, 3600, 6725
SalI GTCGAC 1 cut(s) 3127
SapI GCTCTTC 4 cut(s) 2095, 6061, 6168, 6703
ScaI AGTACT 3 cut(s) 3614, 5010, 5065
SduI GDGCHC 7 cut(s) 62, 266, 1931, 3564, 3760, 6715, 6722
SinI GGWCC 8 cut(s) 2687, 2990, 3833, 4265, 4562, 5330, 6033, 6610
SmiMI CAYNNNNRTG 4 cut(s) 2383, 2858, 3600, 6725
SmlI CTYRAG 6 cut(s) 554, 1666, 2892, 4718, 5589, 5955
SmoI CTYRAG 6 cut(s) 554, 1666, 2892, 4718, 5589, 5955
SpeI ACTAGT 3 cut(s) 3659, 4619, 6368
SphI GCATGC 2 cut(s) 1622, 4400
SspI AATATT 1 cut(s) 6413
StyI CCWWGG 5 cut(s) 1210, 2469, 2853, 6060, 6179
TaiI ACGT 3 cut(s) 305, 2559, 3757
TaqI TCGA 6 cut(s) 2628, 3128, 3194, 4033, 6240, 6906
TatI WGTACW 5 cut(s) 1496, 3612, 4331, 5008, 5063
TauI GCSGC 9 cut(s) 2499, 3146, 3174, 4943, 4946, 4988, 5159, 5255, 6079
TseFI GTSAC 8 cut(s) 1706, 2119, 2193, 2270, 3602, 4645, 4838, 5902
Tsp45I GTSAC 8 cut(s) 1706, 2119, 2193, 2270, 3602, 4645, 4838, 5902
TspGWI ACGGA 6 cut(s) 1688, 3460, 5406, 6428, 6735, 6985
Van91I CCANNNNNTGG 1 cut(s) 6833
VpaK11BI GGWCC 8 cut(s) 2687, 2990, 3833, 4265, 4562, 5330, 6033, 6610
VspI ATTAAT 1 cut(s) 3915
XbaI TCTAGA 6 cut(s) 1231, 1447, 1894, 1901, 4523, 7037
XceI RCATGY 5 cut(s) 1622, 1814, 2120, 4400, 5975
XcmI CCANNNNNNNNNTGG 1 cut(s) 2810
XmiI GTMKAC 2 cut(s) 2216, 3128
XmnI GAANNNNTTC 3 cut(s) 354, 517, 7033
ZrmI AGTACT 3 cut(s) 3614, 5010, 5065
Zsp2I ATGCAT 4 cut(s) 747, 1945, 2452, 5083
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.