MD06G1057100.v1.1

Agenet domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0002656
Physical Location & Seq
Forward (+)
8681952 .. 8695213
13262 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1057100.v1.1.491

Sequence Viewer

Length: 7038 bp
ATGGAGGGAGGTGGATTCTTGTGGTTCTTGGATACACCCATGGATTATGATGACAATGATTTTCAAAGCCAGAATCTTCATTTAGCCGGTGAAGGGAGTACTAATTTTCCGCCAGTTTTACAGCCATATGCTCTTCCCAAGTTTAATTTTGACGATAGCCTTCAAGGGCATTTGAGGTTTGATAGTTTGGTTGAAACCGAGGTTTTTCTGGGTATTGAGAATAACGAACCTAATCACTGGATTGAGGATTTCTCTCGTGGGAGTAGTGGGATAGAGTTTAGTTCCACTGCAGCAGAATCTTGCTCTATATCAAGGCGCAACAATGTTTGGTCTGAAGCCACTTCCTCAGAATCTGTTGAAATGCTATTAAAATCTGTTGGGCAGGAAGAAATTATTCCCTCTGAGACAATCGTTGAGGAATCAGATGCCTGTAAGGAACTGTGTTGCTTAACTGAGCAGATGGAGCCTAATTTGAATAATGATGATAACATTCTTTCTCAAATGGGGGATGTTACAGATCTAGGGCCTACATTACCACGGGTGGAGATTTCCGAAAATCTTTCTGGCTTAAACGATGTAGGAGTAGATCAGCTTTGTGTTGATGATTCACAAACTCGTGAAGGTAAATTATTAGTTGATGGAAATTCAAATAACTTGGAACCAAATGATCTCAGTGGAAAGGACAGCCCAAGTGTAAGTAAAGGGAGTCCTTCTACGTATGGGAAATGCAAAGATGCAAATCAGATGGAATTGGACAATGCGGTTGATGAACGTCTAGATGAAAAAGAAGATTCCTCTGCTTCAGGAATGCAAGTTGATGATATGATATCTGTGCAGAATATTATTACAAGAAGTGATGAGTTGAGTAAAAAAGATGTTCAACATAATTTAAAAGATATCAGTGAAGAGGATCCAGATGGGCATGTGTTGCGCATAGAGGCTCAAGTGAATGAAAAAGTAGTTGAAAAGGCTACCTGTTATCTTGAAAACCCTCATTGTTCATCGTTTGAGGTGGAGTCTGTGGAAGGAGGAATTGCTAATCAGGAAAATGTTGTTAGTGTGGAGGAACCATCGGATATACTGCAAGAGGATTCTGACTTACATACCCTGGGCGGATGCAGTGACAGGGAATGCTCTGGAGCTGATGCTGACACCAACAAATATGAAACTGTGGTCTTGTTTAAAGTCATAGATACTGGTGGGGATCAATCAGAACTAAACACACACAGCTTATCACCTAGGGCGTGTGAAAATGATACTAGCTGCGCAGTTGAGGTTAGCAACAAAAATGCTGAAATTTCTTCAAGTATAGATCCTATGCTGAAAGGGGATTCTGACTTACATGTAGTGGACGTATTCAGTGACAGGGAGTGCTCTGGGGTTCCTGCTGAAACCAACAAATGTGAAGATACAGTCTTGCTTAAAGACTCAGGTAGTGGTGGTGATATTTTTAAATTAAACACAAATGATTTATCACCAATGGTTACCAGAGGCGATGATAGGTTTGCAGGTGAGGTCACCAACAGCAATGCTGGTATTTCTTCAAGTCCATCAAGTCCAGATCCTAAACCGAAATTGGATTCTGGATGGAATAGCTCCAAGGAGAATTCTTTAGAAAGTGGTTTCCAACCAGATAGTGGGACTTTGGTCAGAACGTCCGAGGCTTCTTTGTCAGTCATTGAGGAAAATGAGGTCTCAAAGGATGAAAGTAATGAAAATAGGGAAGTTCATTGTAATTTGACTGCAACATGTTCTTCAGCTGAAGTATTTAGTGAAGCACATGTAACTGGATCTTCTAAAAGTTCTCTTGATGCTTCTGGAATTTCTGGAGAGAAATTGAATGCTGATGGACTTGTATCACTTTCTATTGTTGAGGAGTCTTCTCAAATATGTGATGATAATAAAGTTTACGGAGAAGGTGATGTTGGTGATGGACAGGATGGTAACCTGGATCTCTCTTCCAGTGAAAAGGATAGTACACAATTGCTCAATGAGTCTAATGGTAAATATTTGGAGCTTGGTGGATCTGTTGTTAAGGGATTGGGGTCCTCACCATTGTGTGGAGCTAGCACAGATAAAGAGCTGGTTGTTCCAACATTAACAAATGAAGGTGATGGTAATGAACCGGTGACCAACCTTTTCTTGGAAAATTCGAACGTGGCTTCTTGTGGCACAATGAATGGTGTTCCCTTGTCTTCTGGAAATGGTGTAGCCAGGGATATTGTTAGCAACTCAGAGGTTCAAGCAGCACCTTCTTCTGCTGGGGGTTCATACTGTGACAAAAAAGAAGAAACCGCAAGTGAAATGTCCAAGGACGCAAGTTTTTCATGTATAGTGCCACCTCCTTTGGTAGAAACAGGGCCAGTTTCCGTTTCTGAAGCTGAAAAAGGTGTTTCCTGTGATAGTTCTGGACAGTCGTTATGCAAGACGGTTGATCAATCTCTGCCTGTCACAGACAGTTGCAATACAGAGTGCCAAAATGAACCACAAAGTGCAGTCGCTAATGAAGTTAGCAAGGGAAACACAAATGAGAGGGAAGTAGCTTCTGTTCAGTGTGAATCTTCTACAAAAGTGGGTGATGCTAGTGAAGCTATTTTCTTTGGAAGACAGGATGGCTCAACCATAAAAGAGAGTTTTGAAAAGGCATCTGCAAATGCAACAGAACTAAGTATGGATTCTGACATGCTGCCTGGGCCTTCAACATTGGTGGAAATGTGTGGTGGTACTGCTAAAAATGCAATGGAAGACACTGACACTTCTGAAGTATCTCAGGATAAGACTTCTGCGGAGGCTACTATGCCCAGCATTAATTGTGATGCTTCTCCGATTTGTGAAGCCTCTATCTGTTCTGCTCCTTTGCCTGAATCTCATGCTAGGTTTGTTGCACCAGAAAGTGGTGGAAGTTCTGTTAATCCGAATAAAAATGATTGTGGTTCAACTAAGGTTGTTGAAACAAGTGAGCTTTCTGAGACTAAACATGAATCAGGTGATATCAAGGGGCCCAAAAATCTGAATGCCCCAGTTTCTGACATTGTTAGGAATGGGGACAATCATTCTCCTAAATCCTGGAATCCAAAAGAAAATGATGCCTCCAAGGATTGGAGAAATGGCACGTCGGACGTGAGTTCATTTGCAGATTTGCCAAAAGGGGATGCTCCCAACAACTTGCAGCCCGTTCCTACTATTATACCTCCCAGATTTGCAGAGGGGTCTATACAAAATTCTGGTTCAGGCCAGCTGGATGCAAAAATCTCACAAGATCTTTCTCATGGAGGTTCACTAGTATTTGATGGAGTTGCACGAGGTGCTTCTAAGGTTACCCCTGAACGGAAAACAAGGCGAGCATCTAGCAAGGCAACGGGTAAACAGAGCGCTAAGAAGGGAAGTGCACAAGCAAGAACTCCTATGAGACAATTAGAAAGAGGAGACAGATCAAGCAGTGTGTCCCAAAACCAATCTGGAATTTTCCAGCTTGTGCAGCCTAGTGAGACAAAGCCCTATGGACATGTGGATGGTGCTATAAAACCCTTTTCTGTTCTTAGTACTTCCACATCTAGTTTGCCAGATCTGAATACTTCTGCTCCACCATCTGTAATATTTCAACAGCCGTTCACTGACTTGCAGCAAGTGCAATTACGTGCTCAAATCTTTGTTTATGGAGCTTTAATTCAAGGAATAGCACCTGAAGAAGCTTATATGGTGTCAGCATTTGGGGGACATGATGGTGGAAGGGGCATGTGGGAGAATGCTTGGCGTGTGTCCATAGAGAGGCTACACGGTCAAAAGTCTGCTCTGGGTAATCCAGAAACTCCTTTGCAATCACGCTCGGGTTCCAGGGCTCCTGACCAAGTAATTAAAGGTGCATTCCAGAGTAAAAGTATTCCCTCACCTCTTGGTCGACCCAGCATGAAGGGTGCTCCACCAATGGGTAGCCCAATGATACCTATTTCATCACCTCTTTGGAGTATTTCTACCCCTGTTTGCGAGGGCCTGCAATATAATTATCAGCAGGCACTTAATCCATTGCATCCCTTTCAAACACAATCAATAGGGAACGTTGTTGGGGGCCATAATACTACTTGGATGCCTCAGCCCTCCTTTCGGGGTCCCTGGCTTACTTCTCCACAGTCTTCTGCAGCTCAAGCCAGTACTCATTTTTCAGCATTTCCTAGTACAGAAGCAGTGCAGTTGACTCCTGTAAAAGAAACATCTTTGCCACAATTGCCTAGTAAGAAGCATGTTTCCTCTGGTTCTTCTACTCAGACTGGAGGCCCAACTAGTGTTTTTGCAGGGGTATCTCCAGTGTTTGACCCAAAAAAAGTGTCGGCATCACATGGCCAGCATTCTGCTGACCCTAAGCCTAGAAAAAGAAAAAAGACTTCAGCTTCTGAGGATCATGTTCAGGTTACTATGCAAGCTCGATCTCAACCCGAGTCAGCTCTAACACTTGCTGATAGTAGTAGTCTGTCTACATCTGTTGCCATCTCAACCCCAAGTACCATTGTGTCTAAGACCATGCCAGAGAAATTGATTATGTCTGTCTTTCCAACGCCTTCCACTGGTCGCCTCAAAAAAGCAGATCAGGATTTGGAGCAGAGGGAAGCTTTTACAGAGGCGACTCTTAGTAAAGTTAAGGAGGCTAGGCAACAGGCAGAAGAAGCCGCTGCTTATGCTGCTACAGCTGTTAGTCACAGCCAAGAAATATGGAATCAGTTGGATAAGCAAAAAGATTCCAGATTGATATTTGATGGCGAAGTTACGTTGGCATCTGCTGCTGCAGCTGTAGCTGCCGCGGCTGCTGTTGCAAAGGCAGCAGCTGCAGCTGCCAATGTTGCTTTGAATGCTGCATTACAAGCAAAATTGATGGCTGAAGAAGCCTCGGTTTCATATAATGATGGGAATGCGAGCCAAAGTATAAGAATGACTACTCCTGTGCCCATTTTAAGGGGTGAAGATGTAACTAATAGTTCGACTTCGATCCTTGTTGCTGCTAGGGAGGCTTCTAGAAAGGGAGTTGAAGTTGCATCTGCAGCCTCAAAGCAAGCTGAAAATATGGATGCCATTGTAAAAGCTGCTGAGTTGGCAGCAGAAGCTGTATCACAAGCTGGAATAATTGTAGCTATGGGTGATCCTTTGCCATTGAGTCAGTTAGTAGAAGCTGGTCCAGAGGGTTATTGGAAAGTACCTCAAGTTTCTTCAGGTCTGGTTATGAAATCAAATGGCATGTTGAGAGAACAGTCAAATTTGGGTACTGTCGGAGAAGATGTTGATTCTTCTTCTAGGCGTTCTAAAGATAGACAATCAGATAAGCACAAAGCACAGCCAACTGCACATGAGAAGTCACCTATTCTGACAGAGGTAAATAAGGAATCAATGGATGATCAACTGAGGCCGGGAGTAGGAACCACAGGGTTTGATACTGCCAGTGAAAAGGGGTCAAGAGGACCGAAGGGTCGCAATGTTTCTGAAATCAGATCAAGGTCTGCTTTGATTACTGTCGAGAATGATTTTGAAAAGGAAGCAGGAGCATCTGAAGAAAGCAGCATCAAGGAGGGTTCTCTAGTAGAGGTTCTCAAAGATGGGGCTGGATTTGGAGAAGCCTGGTTTACTGCTAAGGTATTGTGTTTGCAAAATGGAAAAGCTAGTGTGTGTTACACTGAGCTTCCGTCAGATGAAGGGAAACTGCAGGAATGGGTGGCACTTGATGGCGAAGAAGATAAGCCACCAAAGATACGAATTGCCCGCCCTGTTACCGCTTTGGGACATGACGGAACAAGGAAAAGGCGCAGAGCAGCAATGGCAGATTATACTTGGTCTGTTGGAGATAAAGTTGATGCATGGATACAGGAGAGCTGGTGGGAAGGAGTTGTCACTGAAAAAAACAAGAAAGATGAAACTATATTAACGGTCCACTTTCCAGCTCAAGGGGAAAAGTCAGTTGTTAAAGCTTGGCATCTTCGGCCTTCGCTCATTTGGAAGGATGGCAAATGGGTTGAATGGTTTAGTGTACGAAATGACACCTCCTTCCTTGAGGGTGATATGCCCCAGGAAAAGCGACCCAAATTTGGCAGTCCTGCAGTGGAAGACAAGGGGAATGATAACGCGTCAAAAAGCATTGATGTAAATGATTCAGGGAAACCTGTAGACCCAAGGTTACTGAATTTATCTGCAAATGAAAAGGTATTTAATATGGGTAAGAATACCAGAACTGAGAATAAGCTTGATGCAACCAGAACAATTCGGACAGGGTTGCAGAAGGAAAGATCAAGGGGGGTTGTTTTTGGTATCCCCAAGCCTGGAAAGAAGAGAAAATTCATGGAAGTTAGCAAACATTATGTGGAAAATGAGGGTGGCAAGATTAATGAAACTAGTGATCCGGTGAAAATTGCAAAATACTTGATGCCTCAAGGATCAGGTTCCCGTGGATTGAAAAATACATCCAAAATCGACACGAGGGAAAAACAAGTGGCTGGATCCAAGCTAAAGGGTCTGAGATCTGGAAAGCTGCAAAGTTTATCTGGTAAATCTGCTGCACAGAAGGACCACCTCTTAACAGATGCACACACTGCCTCTGATGGTTCAAGTGAAATGGACCATACAGGAAAGATCAAAGATTCTGTAAACCATGCTGAGGGTTTATCCGGAAAGCATACACTATCACAAACATCTACACATAGAACAGAAGGGACAACAGACGGCCCAATGGAATTTTCTTCATTAGCTCCATCATCTGATTCTTCTTCATCCAAGAAAGTCTCCACTTTAACTGCTCTATCTCGGGCAAACAAAGGAAAACTTGCACCTGCTGGTGGAAGGTTGGGTAAAATTGAGGAGGGCAAAGTGTTTAGTGGAAATCCGGCAAAGTCGACTGCTGAAGTTGTTGAGCCTCGTAGATCAAATCGCAGAATTCAACCTACATCAAGACTACTAGAAGGACTACAAAGCTCGTTGATCATCTCAAAGATTCCTTCAGTTTCACATGACAAGGGCCACAGAAGTCAGAACCGGAATGCCTCTAGAGGGAATAACAATGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Agenet

Protein Analysis

2346

Amino Acids

250.09

Weight (kDa)

4.99

Isoelectric Point (pI)

46.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Agenet PF05641 1852 - 1912 5.7e-08 Agenet domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 1499, 6811
AasI GACNNNNNNGTC 1 cut(s) 5451
Acc16I TGCGCA 2 cut(s) 932, 1267
Acc36I ACCTGC 2 cut(s) 1499, 6811
AccB7I CCANNNNNTGG 4 cut(s) 1637, 2060, 2894, 3099
AccI GTMKAC 4 cut(s) 3898, 4469, 6144, 6866
AccII CGCG 2 cut(s) 4793, 6104
AccIII TCCGGA 1 cut(s) 6641
AclI AACGTT 1 cut(s) 4056
AcoI YGGCCR 1 cut(s) 4336
AdeI CACNNNGTG 2 cut(s) 2492, 3305
AfeI AGCGCT 1 cut(s) 3373
AflIII ACRYGT 5 cut(s) 1342, 1748, 1780, 3505, 6102
AgeI ACCGGT 1 cut(s) 2125
AhlI ACTAGT 3 cut(s) 3280, 4277, 6368
AjiI CACGTC 2 cut(s) 3114, 3121
AjuI GAANNNNNNNTTGG 4 cut(s) 1908, 1940, 6694, 6726
AleI CACNNNNGTG 1 cut(s) 2056
Alw21I GWGCWC 4 cut(s) 1376, 3391, 3643, 3919
Alw26I GTCTC 7 cut(s) 398, 1699, 2963, 3403, 3420, 3482, 6760
Alw44I GTGCAC 1 cut(s) 3387
AlwNI CAGNNNCTG 5 cut(s) 353, 3026, 4388, 4817, 5093
Ama87I CYCGRG 3 cut(s) 3826, 4430, 6777
Aor13HI TCCGGA 1 cut(s) 6641
Aor51HI AGCGCT 1 cut(s) 3373
ApaI GGGCCC 1 cut(s) 3003
ApaLI GTGCAC 1 cut(s) 3387
ArsI GACNNNNNNTTYG 2 cut(s) 4307, 4339
AseI ATTAAT 2 cut(s) 2808, 6360
AsiGI ACCGGT 1 cut(s) 2125
Asp700I GAANNNNTTC 1 cut(s) 393
AspA2I CCTAGG 1 cut(s) 1238
AspLEI GCGC 5 cut(s) 318, 933, 1268, 3374, 5787
AsuC2I CCSGG 1 cut(s) 5394
AsuII TTCGAA 1 cut(s) 2153
AsuNHI GCTAGC 1 cut(s) 2066
AvaI CYCGRG 3 cut(s) 3826, 4430, 6777
AvaII GGWCC 7 cut(s) 2046, 4106, 5162, 5444, 5908, 6541, 6592
AvrII CCTAGG 1 cut(s) 1238
BaeGI GKGCMC 3 cut(s) 3003, 3391, 4938
BalI TGGCCA 1 cut(s) 4338
BamHI GGATCC 2 cut(s) 910, 6473
BanII GRGCYC 2 cut(s) 3003, 3841
BauI CACGAG 4 cut(s) 255, 615, 3300, 6451
BbsI GAAGAC 6 cut(s) 1872, 2187, 2611, 2751, 4122, 6090
Bbv12I GWGCWC 4 cut(s) 1376, 3391, 3643, 3919
BbvCI CCTCAGC 2 cut(s) 4089, 6630
BceAI ACGGC 2 cut(s) 3592, 6712
BciVI GTATCC 3 cut(s) 25, 5835, 6296
BclI TGATCA 3 cut(s) 2434, 5380, 6951
BcnI CCSGG 1 cut(s) 5394
BcoDI GTCTC 7 cut(s) 398, 1699, 2963, 3403, 3420, 3482, 6760
BcuI ACTAGT 3 cut(s) 3280, 4277, 6368
BfoI RGCGCY 1 cut(s) 3375
BfuAI ACCTGC 2 cut(s) 1499, 6811
BfuI GTATCC 3 cut(s) 25, 5835, 6296
BglII AGATCT 4 cut(s) 517, 3259, 3565, 6494
BlnI CCTAGG 1 cut(s) 1238
BmcAI AGTACT 3 cut(s) 100, 3544, 4150
Bme18I GGWCC 7 cut(s) 2046, 4106, 5162, 5444, 5908, 6541, 6592
BmeT110I CYCGRG 3 cut(s) 3826, 4430, 6777
BmgBI CACGTC 2 cut(s) 3114, 3121
BmrI ACTGGG 1 cut(s) 3014
BmtI GCTAGC 1 cut(s) 2070
BmuI ACTGGG 1 cut(s) 3014
BoxI GACNNNNGTC 1 cut(s) 1646
BpiI GAAGAC 6 cut(s) 1872, 2187, 2611, 2751, 4122, 6090
BplI GAGNNNNNCTC 2 cut(s) 236, 268
BpmI CTGGAG 4 cut(s) 1158, 1848, 4284, 4287
Bpu10I CCTNAGC 4 cut(s) 4089, 4356, 5613, 6630
Bpu14I TTCGAA 1 cut(s) 2153
BpuEI CTTGAG 6 cut(s) 927, 4125, 5172, 5907, 6050, 6390
BpuMI CCSGG 1 cut(s) 5394
BsaAI YACGTR 2 cut(s) 717, 3638
BsaBI GATNNNNATC 1 cut(s) 738
BsaI GGTCTC 1 cut(s) 1699
BsaWI WCCGGW 4 cut(s) 2125, 6376, 6641, 7005
Bse118I RCCGGY 2 cut(s) 86, 2125
Bse3DI GCAATG 5 cut(s) 1534, 2745, 4022, 5464, 5802
Bse8I GATNNNNATC 1 cut(s) 738
BseAI TCCGGA 1 cut(s) 6641
BseJI GATNNNNATC 1 cut(s) 738
BseMI GCAATG 5 cut(s) 1534, 2745, 4022, 5464, 5802
BseRI GAGGAG 3 cut(s) 1889, 3438, 6845
BseSI GKGCMC 3 cut(s) 3003, 3391, 4938
BseYI CCCAGC 3 cut(s) 2261, 2801, 3902
BsgI GTGCAG 6 cut(s) 854, 2514, 3497, 4205, 5313, 6516
Bsh1236I CGCG 2 cut(s) 4793, 6104
BshTI ACCGGT 1 cut(s) 2125
BsiHKAI GWGCWC 4 cut(s) 1376, 3391, 3643, 3919
BsiHKCI CYCGRG 3 cut(s) 3826, 4430, 6777
BsiSI CCGG 7 cut(s) 87, 2126, 5393, 6377, 6642, 6857, 7006
BslFI GGGAC 7 cut(s) 1654, 3059, 3430, 3729, 4092, 5775, 6700
BsmAI GTCTC 7 cut(s) 398, 1699, 2963, 3403, 3420, 3482, 6760
BsmFI GGGAC 7 cut(s) 1654, 3059, 3430, 3729, 4092, 5775, 6700
Bso31I GGTCTC 1 cut(s) 1699
BsoBI CYCGRG 3 cut(s) 3826, 4430, 6777
Bsp119I TTCGAA 1 cut(s) 2153
Bsp120I GGGCCC 1 cut(s) 2999
Bsp1286I GDGCHC 7 cut(s) 1376, 3003, 3391, 3643, 3841, 3919, 4938
Bsp13I TCCGGA 1 cut(s) 6641
Bsp19I CCATGG 1 cut(s) 39
BspEI TCCGGA 1 cut(s) 6641
BspFNI CGCG 2 cut(s) 4793, 6104
BspMAI CTGCAG 7 cut(s) 292, 4138, 4780, 4822, 5032, 5688, 6079
BspMI ACCTGC 2 cut(s) 1499, 6811
BspOI GCTAGC 1 cut(s) 2070
BspQI GCTCTTC 1 cut(s) 138
BspT104I TTCGAA 1 cut(s) 2153
BspTNI GGTCTC 1 cut(s) 1699
BsrDI GCAATG 5 cut(s) 1534, 2745, 4022, 5464, 5802
BsrFI RCCGGY 2 cut(s) 86, 2125
BssAI RCCGGY 2 cut(s) 86, 2125
BssSI CACGAG 4 cut(s) 255, 615, 3300, 6451
BssT1I CCWWGG 6 cut(s) 39, 1238, 1599, 2310, 3093, 6149
Bst2BI CACGAG 4 cut(s) 255, 615, 3300, 6451
Bst6I CTCTTC 4 cut(s) 138, 900, 1963, 6299
BstAPI GCANNNNNTGC 6 cut(s) 928, 3305, 3628, 4772, 4817, 6566
BstBAI YACGTR 2 cut(s) 717, 3638
BstBI TTCGAA 1 cut(s) 2153
BstDSI CCRYGG 4 cut(s) 39, 536, 4791, 6421
BstEII GGTNACC 5 cut(s) 1483, 1516, 1943, 2128, 3316
BstFNI CGCG 2 cut(s) 4793, 6104
BstH2I RGCGCY 1 cut(s) 3375
BstHHI GCGC 5 cut(s) 318, 933, 1268, 3374, 5787
BstMAI GTCTC 7 cut(s) 398, 1699, 2963, 3403, 3420, 3482, 6760
BstNSI RCATGY 9 cut(s) 926, 1346, 1752, 1784, 2686, 3509, 3739, 4241, 5227
BstPAI GACNNNNGTC 1 cut(s) 1646
BstPI GGTNACC 5 cut(s) 1483, 1516, 1943, 2128, 3316
BstSLI GKGCMC 3 cut(s) 3003, 3391, 4938
BstSNI TACGTA 1 cut(s) 717
BstUI CGCG 2 cut(s) 4793, 6104
BstV2I GAAGAC 6 cut(s) 1872, 2187, 2611, 2751, 4122, 6090
BsuI GTATCC 3 cut(s) 25, 5835, 6296
BtgI CCRYGG 4 cut(s) 39, 536, 4791, 6421
BtgZI GCGATG 1 cut(s) 1509
BtrI CACGTC 2 cut(s) 3114, 3121
BtsI GCAGTG 6 cut(s) 285, 1126, 3445, 4188, 6084, 6564
BveI ACCTGC 2 cut(s) 1499, 6811
CaiI CAGNNNCTG 5 cut(s) 353, 3026, 4388, 4817, 5093
CfoI GCGC 5 cut(s) 318, 933, 1268, 3374, 5787
Cfr10I RCCGGY 2 cut(s) 86, 2125
Cfr42I CCGCGG 1 cut(s) 4794
CseI GACGC 2 cut(s) 2324, 6093
CspAI ACCGGT 1 cut(s) 2125
CspCI CAANNNNNGTGG 6 cut(s) 2328, 2363, 2688, 2723, 5395, 5430
DraI TTTAAA 3 cut(s) 891, 1183, 1453
DraIII CACNNNGTG 2 cut(s) 2492, 3305
DrdI GACNNNNNNGTC 1 cut(s) 5451
DseDI GACNNNNNNGTC 1 cut(s) 5451
EaeI YGGCCR 1 cut(s) 4336
Eam1104I CTCTTC 4 cut(s) 138, 900, 1963, 6299
EarI CTCTTC 4 cut(s) 138, 900, 1963, 6299
EciI GGCGGA 2 cut(s) 99, 1128
Eco105I TACGTA 1 cut(s) 717
Eco130I CCWWGG 6 cut(s) 39, 1238, 1599, 2310, 3093, 6149
Eco24I GRGCYC 2 cut(s) 3003, 3841
Eco31I GGTCTC 1 cut(s) 1699
Eco32I GATATC 3 cut(s) 828, 898, 2992
Eco47I GGWCC 7 cut(s) 2046, 4106, 5162, 5444, 5908, 6541, 6592
Eco47III AGCGCT 1 cut(s) 3373
Eco88I CYCGRG 3 cut(s) 3826, 4430, 6777
Eco91I GGTNACC 5 cut(s) 1483, 1516, 1943, 2128, 3316
EcoO109I RGGNCCY 5 cut(s) 524, 2046, 2999, 3988, 4106
EcoO65I GGTNACC 5 cut(s) 1483, 1516, 1943, 2128, 3316
EcoRI GAATTC 2 cut(s) 1606, 6906
EcoRV GATATC 3 cut(s) 828, 898, 2992
EcoT14I CCWWGG 6 cut(s) 39, 1238, 1599, 2310, 3093, 6149
EcoT22I ATGCAT 1 cut(s) 5839
EcoT38I GRGCYC 2 cut(s) 3003, 3841
ErhI CCWWGG 6 cut(s) 39, 1238, 1599, 2310, 3093, 6149
FalI AAGNNNNNCTT 4 cut(s) 5470, 5502, 6780, 6812
FaqI GGGAC 7 cut(s) 1654, 3059, 3430, 3729, 4092, 5775, 6700
FauI CCCGC 1 cut(s) 5750
FauNDI CATATG 1 cut(s) 127
FbaI TGATCA 3 cut(s) 2434, 5380, 6951
FblI GTMKAC 4 cut(s) 3898, 4469, 6144, 6866
FriOI GRGCYC 2 cut(s) 3003, 3841
FspI TGCGCA 2 cut(s) 932, 1267
GlaI GCGC 5 cut(s) 317, 932, 1267, 3373, 5786
GsaI CCCAGC 3 cut(s) 2265, 2805, 3906
GsuI CTGGAG 4 cut(s) 1158, 1848, 4284, 4287
HaeII RGCGCY 1 cut(s) 3375
HapII CCGG 7 cut(s) 87, 2126, 5393, 6377, 6642, 6857, 7006
HgaI GACGC 2 cut(s) 2324, 6093
HhaI GCGC 5 cut(s) 318, 933, 1268, 3374, 5787
Hin6I GCGC 5 cut(s) 316, 931, 1266, 3372, 5785
HinP1I GCGC 5 cut(s) 316, 931, 1266, 3372, 5785
HincII GTYRAC 3 cut(s) 3899, 4191, 6867
HindII GTYRAC 3 cut(s) 3899, 4191, 6867
HindIII AAGCTT 4 cut(s) 3690, 4602, 5946, 6218
HpaII CCGG 7 cut(s) 87, 2126, 5393, 6377, 6642, 6857, 7006
Hpy99I CGWCG 1 cut(s) 3118
HspAI GCGC 5 cut(s) 316, 931, 1266, 3372, 5785
KflI GGGWCCC 1 cut(s) 4106
Kpn2I TCCGGA 1 cut(s) 6641
Ksp22I TGATCA 3 cut(s) 2434, 5380, 6951
KspI CCGCGG 1 cut(s) 4794
LguI GCTCTTC 1 cut(s) 138
MfeI CAATTG 2 cut(s) 1982, 4220
MhlI GDGCHC 7 cut(s) 1376, 3003, 3391, 3643, 3841, 3919, 4938
MlsI TGGCCA 1 cut(s) 4338
MluI ACGCGT 1 cut(s) 6102
MluNI TGGCCA 1 cut(s) 4338
MlyI GAGTC 9 cut(s) 715, 1025, 1421, 1886, 2003, 4186, 4442, 4612, 5152
MmeI TCCRAC 7 cut(s) 1651, 2117, 3096, 4571, 4695, 5236, 5800
Mox20I TGGCCA 1 cut(s) 4338
Mph1103I ATGCAT 1 cut(s) 5839
MroI TCCGGA 1 cut(s) 6641
MroXI GAANNNNTTC 1 cut(s) 393
MscI TGGCCA 1 cut(s) 4338
MslI CAYNNNNRTG 3 cut(s) 2056, 3510, 3723
Msp20I TGGCCA 1 cut(s) 4338
MspA1I CMGCKG 8 cut(s) 1760, 3238, 4664, 4682, 4781, 4793, 4817, 4823
MspI CCGG 7 cut(s) 87, 2126, 5393, 6377, 6642, 6857, 7006
MunI CAATTG 2 cut(s) 1982, 4220
MvnI CGCG 2 cut(s) 4793, 6104
NciI CCSGG 1 cut(s) 5394
NcoI CCATGG 1 cut(s) 39
NdeI CATATG 1 cut(s) 127
NheI GCTAGC 1 cut(s) 2066
NmuCI GTSAC 9 cut(s) 1121, 1361, 1516, 2128, 2276, 2449, 4688, 5340, 5869
NsbI TGCGCA 2 cut(s) 932, 1267
NsiI ATGCAT 1 cut(s) 5839
NspI RCATGY 9 cut(s) 926, 1346, 1752, 1784, 2686, 3509, 3739, 4241, 5227
NspV TTCGAA 1 cut(s) 2153
OliI CACNNNNGTG 1 cut(s) 2056
PaqCI CACCTGC 2 cut(s) 1499, 6811
PasI CCCWGGG 1 cut(s) 1108
PciI ACATGT 4 cut(s) 1342, 1748, 1780, 3505
PciSI GCTCTTC 1 cut(s) 138
PdmI GAANNNNTTC 1 cut(s) 393
PflMI CCANNNNNTGG 4 cut(s) 1637, 2060, 2894, 3099
PfoI TCCNGGA 1 cut(s) 3065
PinAI ACCGGT 1 cut(s) 2125
PleI GAGTC 9 cut(s) 714, 1024, 1421, 1885, 2002, 4186, 4441, 4612, 5151
PpsI GAGTC 9 cut(s) 714, 1024, 1421, 1885, 2002, 4186, 4441, 4612, 5151
Ppu21I YACGTR 2 cut(s) 717, 3638
PpuMI RGGWCCY 2 cut(s) 2046, 4106
PscI ACATGT 4 cut(s) 1342, 1748, 1780, 3505
PshAI GACNNNNGTC 1 cut(s) 1646
PshBI ATTAAT 2 cut(s) 2808, 6360
Psp1406I AACGTT 1 cut(s) 4056
Psp5II RGGWCCY 2 cut(s) 2046, 4106
PspEI GGTNACC 5 cut(s) 1483, 1516, 1943, 2128, 3316
PspFI CCCAGC 3 cut(s) 2261, 2801, 3902
PspOMI GGGCCC 1 cut(s) 2999
PspPPI RGGWCCY 2 cut(s) 2046, 4106
PsrI GAACNNNNNNTAC 2 cut(s) 2532, 2564
PstI CTGCAG 7 cut(s) 292, 4138, 4780, 4822, 5032, 5688, 6079
PstNI CAGNNNCTG 5 cut(s) 353, 3026, 4388, 4817, 5093
PvuII CAGCTG 6 cut(s) 1760, 3238, 4682, 4781, 4817, 4823
RseI CAYNNNNRTG 3 cut(s) 2056, 3510, 3723
SacII CCGCGG 1 cut(s) 4794
SalI GTCGAC 2 cut(s) 3897, 6865
SapI GCTCTTC 1 cut(s) 138
ScaI AGTACT 3 cut(s) 100, 3544, 4150
SchI GAGTC 9 cut(s) 715, 1025, 1421, 1886, 2003, 4186, 4442, 4612, 5152
SduI GDGCHC 7 cut(s) 1376, 3003, 3391, 3643, 3841, 3919, 4938
Sfr303I CCGCGG 1 cut(s) 4794
SfuI TTCGAA 1 cut(s) 2153
SgrBI CCGCGG 1 cut(s) 4794
SinI GGWCC 7 cut(s) 2046, 4106, 5162, 5444, 5908, 6541, 6592
SmiMI CAYNNNNRTG 3 cut(s) 2056, 3510, 3723
SmlI CTYRAG 6 cut(s) 942, 4140, 5187, 5922, 6029, 6405
SmoI CTYRAG 6 cut(s) 942, 4140, 5187, 5922, 6029, 6405
SnaBI TACGTA 1 cut(s) 717
SpeI ACTAGT 3 cut(s) 3280, 4277, 6368
SspI AATATT 3 cut(s) 841, 2009, 3597
StyI CCWWGG 6 cut(s) 39, 1238, 1599, 2310, 3093, 6149
TaqI TCGA 8 cut(s) 2153, 3898, 4420, 4970, 4976, 5499, 6447, 6866
TaqII GACCGA 1 cut(s) 5461
TatI WGTACW 5 cut(s) 98, 1976, 3542, 4148, 4172
TauI GCSGC 3 cut(s) 4664, 4793, 4796
TseFI GTSAC 9 cut(s) 1121, 1361, 1516, 2128, 2276, 2449, 4688, 5340, 5869
Tsp45I GTSAC 9 cut(s) 1121, 1361, 1516, 2128, 2276, 2449, 4688, 5340, 5869
TspGWI ACGGA 5 cut(s) 1926, 2359, 3343, 5655, 5784
Van91I CCANNNNNTGG 4 cut(s) 1637, 2060, 2894, 3099
VneI GTGCAC 1 cut(s) 3387
VpaK11BI GGWCC 7 cut(s) 2046, 4106, 5162, 5444, 5908, 6541, 6592
VspI ATTAAT 2 cut(s) 2808, 6360
XbaI TCTAGA 3 cut(s) 775, 5003, 7016
XceI RCATGY 9 cut(s) 926, 1346, 1752, 1784, 2686, 3509, 3739, 4241, 5227
XcmI CCANNNNNNNNNTGG 4 cut(s) 1634, 2140, 3455, 5172
XmaJI CCTAGG 1 cut(s) 1238
XmiI GTMKAC 4 cut(s) 3898, 4469, 6144, 6866
XmnI GAANNNNTTC 1 cut(s) 393
ZrmI AGTACT 3 cut(s) 100, 3544, 4150
Zsp2I ATGCAT 1 cut(s) 5839
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.